FvH4_2g14201

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0002523
Physical Location & Seq
Forward (+)
12470205 .. 12473081
2877 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14201.t2

Sequence Viewer

Length: 960 bp
ATGGAGGATGCCCAAGAAGTATCTGAGACAATGTCAGAGAGCTCATCAGGAAAGATACAGGAAACTCGCGATGAAATGCTCTCCAGGCACAGGAAAGAGACTTCAAAGTTGCAGGACAAGGAAATTCAATTGAAAAAGGCAGCAGCAAAAGGTAGCAAGGCCGAACAAAAAGCTAAGAAAAAGCAGGTGGAGGAAGAGATTTCTCGACTTTCTGCTGAGCTTAAGGAAAAACAAGCCGAAGAACTTGCATTATTAGGCTATAGCAGCAGTAACAATGGAGGTGAAAAGAGTAATCTGGATACTTTAGTGAAGGCCATAGCTGGTGTTTCTGTCAGCAGTCAACCTGAGAGTGCAAAGCCTAGCAAGAGTTCAAAGAGAAAAGTGAAAAGGGCCCAGGAGGATGCAGCAAGGGAGCAAAGAATTCAAGAAGAGCAGAGCAACTTAGTAAGTGATCGAATGGTTGAGGATGAGAAACTAGAAGAAAAGCTTGAACCCCTAGGTTTGACTATCAATGAGATAAAGCCTGATGGTCATTGCCTCTACCGAGCTATCCAGGATCAGCTGGCCCACCATTCTGGAGGCTCATCCCCTTACACATACCAAGAGCTTCGAGAAATGGTGGCAGCTTATATGAGGAAGCATGCACCTGATTTTCTCCCATTCTTCTTGTCAGACAACCAAGTCGATGGTGATTCTGATGAATCGCTTGCTGAAAGGTTTGAGAATTACTGTAAGGAAGTGGAGTCAACAGCAGCATGGGGAGGACAGCTAGAGCTTGGGGCTTTAACTCACTGCCTTAAAAAGCCTATCATGGTATACTCAGGATCATTCCCTGATGTGGAGATGGGTAAGGAGTACAAATCTGAGAGTGGAAATGGTTCTGTTTCTAGTATTATGTTGTCATATCATAAGCACGCCTTCGGGCTTGGAGAGCACTATAATTCTGTGGTCCCTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

320

Amino Acids

35.52

Weight (kDa)

5.46

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 213 - 352 1.7e-35 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 175
AasI GACNNNNNNGTC 1 cut(s) 680
Acc36I ACCTGC 1 cut(s) 175
AccI GTMKAC 1 cut(s) 816
AccII CGCG 1 cut(s) 69
AclWI GGATC 2 cut(s) 564, 832
AcsI RAATTY 2 cut(s) 123, 420
AfaI GTAC 1 cut(s) 857
AfiI CCNNNNNNNGG 2 cut(s) 90, 838
AflII CTTAAG 1 cut(s) 221
AgsI TTSAA 6 cut(s) 105, 128, 133, 372, 425, 491
AjnI CCWGG 3 cut(s) 83, 393, 552
Alw21I GWGCWC 2 cut(s) 44, 936
Alw26I GTCTC 2 cut(s) 20, 92
AlwI GGATC 2 cut(s) 564, 832
AoxI GGCC 4 cut(s) 159, 312, 390, 564
ApaI GGGCCC 1 cut(s) 394
ApeKI GCWGC 6 cut(s) 140, 143, 264, 404, 623, 752
ApoI RAATTY 2 cut(s) 123, 420
Asp700I GAANNNNTTC 1 cut(s) 877
AspA2I CCTAGG 1 cut(s) 496
AspS9I GGNCC 4 cut(s) 390, 391, 565, 949
AsuHPI GGTGA 2 cut(s) 293, 701
AvaII GGWCC 1 cut(s) 949
AvrII CCTAGG 1 cut(s) 496
BaeGI GKGCMC 1 cut(s) 394
BaeI ACNNNNGTAYC 2 cut(s) 291, 324
BanII GRGCYC 2 cut(s) 44, 394
Bbv12I GWGCWC 2 cut(s) 44, 936
BbvI GCAGC 6 cut(s) 152, 155, 276, 416, 635, 764
BccI CCATC 3 cut(s) 521, 680, 838
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 3 cut(s) 85, 395, 554
BciVI GTATCC 1 cut(s) 292
BcoDI GTCTC 2 cut(s) 20, 92
BfaI CTAG 5 cut(s) 360, 476, 497, 770, 888
BfmI CTRYAG 1 cut(s) 259
BfrI CTTAAG 1 cut(s) 221
BfuAI ACCTGC 1 cut(s) 175
BfuI GTATCC 1 cut(s) 292
BisI GCNGC 6 cut(s) 141, 144, 265, 405, 624, 753
BlnI CCTAGG 1 cut(s) 496
BlpI GCTNAGC 1 cut(s) 216
BlsI GCNGC 6 cut(s) 142, 145, 266, 406, 625, 754
Bme1390I CCNGG 3 cut(s) 85, 395, 554
Bme18I GGWCC 1 cut(s) 949
BmgT120I GGNCC 4 cut(s) 390, 391, 565, 949
BmiI GGNNCC 2 cut(s) 392, 951
BmrFI CCNGG 3 cut(s) 85, 395, 554
BmsI GCATC 1 cut(s) 391
BpmI CTGGAG 2 cut(s) 67, 597
Bpu1102I GCTNAGC 1 cut(s) 216
BsaJI CCNNGG 2 cut(s) 393, 496
Bsc4I CCNNNNNNNGG 2 cut(s) 90, 838
Bse3DI GCAATG 1 cut(s) 532
BseBI CCWGG 3 cut(s) 85, 395, 554
BseDI CCNNGG 2 cut(s) 393, 496
BseGI GGATG 4 cut(s) 13, 406, 472, 584
BseLI CCNNNNNNNGG 2 cut(s) 90, 838
BseMI GCAATG 1 cut(s) 532
BseMII CTCAG 5 cut(s) 15, 207, 336, 834, 855
BseSI GKGCMC 1 cut(s) 394
BseXI GCAGC 6 cut(s) 152, 155, 276, 416, 635, 764
Bsh1236I CGCG 1 cut(s) 69
BshFI GGCC 4 cut(s) 161, 314, 392, 566
BsiHKAI GWGCWC 2 cut(s) 44, 936
BslFI GGGAC 1 cut(s) 935
BslI CCNNNNNNNGG 2 cut(s) 90, 838
BsmAI GTCTC 2 cut(s) 20, 92
BsmFI GGGAC 1 cut(s) 935
BsnI GGCC 4 cut(s) 161, 314, 392, 566
Bsp120I GGGCCC 1 cut(s) 390
Bsp1286I GDGCHC 3 cut(s) 44, 394, 936
Bsp143I GATC 3 cut(s) 451, 556, 824
Bsp1720I GCTNAGC 1 cut(s) 216
Bsp68I TCGCGA 1 cut(s) 69
BspANI GGCC 4 cut(s) 161, 314, 392, 566
BspCNI CTCAG 5 cut(s) 16, 208, 337, 833, 856
BspFNI CGCG 1 cut(s) 69
BspLI GGNNCC 2 cut(s) 392, 951
BspMI ACCTGC 1 cut(s) 175
BspPI GGATC 2 cut(s) 564, 832
BspQI GCTCTTC 1 cut(s) 423
BspTI CTTAAG 1 cut(s) 221
BsrDI GCAATG 1 cut(s) 532
BssECI CCNNGG 2 cut(s) 393, 496
BssMI GATC 3 cut(s) 451, 556, 824
BssNAI GTATAC 1 cut(s) 817
BssT1I CCWWGG 1 cut(s) 496
Bst1107I GTATAC 1 cut(s) 817
Bst2UI CCWGG 3 cut(s) 85, 395, 554
Bst4CI ACNGT 1 cut(s) 731
Bst6I CTCTTC 2 cut(s) 189, 423
BstAFI CTTAAG 1 cut(s) 221
BstC8I GCNNGC 4 cut(s) 564, 642, 708, 915
BstDEI CTNAG 7 cut(s) 24, 174, 216, 345, 442, 820, 864
BstF5I GGATG 4 cut(s) 13, 406, 472, 584
BstFNI CGCG 1 cut(s) 69
BstKTI GATC 3 cut(s) 454, 559, 827
BstMAI GTCTC 2 cut(s) 20, 92
BstMBI GATC 3 cut(s) 451, 556, 824
BstMWI GCNNNNNNNGC 3 cut(s) 85, 264, 931
BstNI CCWGG 3 cut(s) 85, 395, 554
BstNSI RCATGY 1 cut(s) 644
BstSCI CCNGG 3 cut(s) 83, 393, 552
BstSFI CTRYAG 1 cut(s) 259
BstSLI GKGCMC 1 cut(s) 394
BstUI CGCG 1 cut(s) 69
BstV1I GCAGC 6 cut(s) 152, 155, 276, 416, 635, 764
BstXI CCANNNNNNTGG 2 cut(s) 575, 686
BstZ17I GTATAC 1 cut(s) 817
BsuI GTATCC 1 cut(s) 292
BsuRI GGCC 4 cut(s) 161, 314, 392, 566
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 4 cut(s) 13, 406, 472, 584
BtsI GCAGTG 1 cut(s) 790
BtsIMutI CAGTG 1 cut(s) 790
BtuMI TCGCGA 1 cut(s) 69
BveI ACCTGC 1 cut(s) 175
Cac8I GCNNGC 4 cut(s) 564, 642, 708, 915
Cfr13I GGNCC 4 cut(s) 390, 391, 565, 949
Csp6I GTAC 1 cut(s) 856
CviAII CATG 3 cut(s) 641, 756, 811
CviQI GTAC 1 cut(s) 856
DdeI CTNAG 7 cut(s) 24, 174, 216, 345, 442, 820, 864
DpnI GATC 3 cut(s) 453, 558, 826
DpnII GATC 3 cut(s) 451, 556, 824
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
Eam1104I CTCTTC 2 cut(s) 189, 423
EarI CTCTTC 2 cut(s) 189, 423
Ecl136II GAGCTC 1 cut(s) 42
Eco130I CCWWGG 1 cut(s) 496
Eco24I GRGCYC 2 cut(s) 44, 394
Eco47I GGWCC 1 cut(s) 949
Eco53kI GAGCTC 1 cut(s) 42
EcoICRI GAGCTC 1 cut(s) 42
EcoO109I RGGNCCY 1 cut(s) 390
EcoRI GAATTC 1 cut(s) 420
EcoRII CCWGG 3 cut(s) 83, 393, 552
EcoT14I CCWWGG 1 cut(s) 496
EcoT38I GRGCYC 2 cut(s) 44, 394
ErhI CCWWGG 1 cut(s) 496
FaeI CATG 3 cut(s) 644, 759, 814
FalI AAGNNNNNCTT 4 cut(s) 471, 503, 902, 934
FaqI GGGAC 1 cut(s) 935
FatI CATG 3 cut(s) 640, 755, 810
FblI GTMKAC 1 cut(s) 816
Fnu4HI GCNGC 6 cut(s) 141, 144, 265, 405, 624, 753
FokI GGATG 4 cut(s) 20, 413, 479, 571
FriOI GRGCYC 2 cut(s) 44, 394
Fsp4HI GCNGC 6 cut(s) 141, 144, 265, 405, 624, 753
FspBI CTAG 5 cut(s) 360, 476, 497, 770, 888
GluI GCNGC 6 cut(s) 141, 144, 265, 405, 624, 753
GsuI CTGGAG 2 cut(s) 67, 597
HaeIII GGCC 4 cut(s) 161, 314, 392, 566
Hin1II CATG 3 cut(s) 644, 759, 814
HincII GTYRAC 2 cut(s) 341, 747
HindII GTYRAC 2 cut(s) 341, 747
HindIII AAGCTT 1 cut(s) 485
HinfI GANTC 3 cut(s) 692, 701, 743
HphI GGTGA 2 cut(s) 293, 701
Hpy166II GTNNAC 3 cut(s) 341, 747, 817
Hpy188I TCNGA 5 cut(s) 25, 37, 673, 697, 865
Hpy188III TCNNGA 8 cut(s) 48, 68, 204, 296, 425, 576, 611, 822
Hpy8I GTNNAC 3 cut(s) 341, 747, 817
HpyAV CCTTC 2 cut(s) 304, 928
HpyCH4III ACNGT 1 cut(s) 731
HpyCH4V TGCA 5 cut(s) 112, 248, 353, 404, 644
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 264, 931
HpyF3I CTNAG 7 cut(s) 24, 174, 216, 345, 442, 820, 864
Hsp92II CATG 3 cut(s) 644, 759, 814
Kzo9I GATC 3 cut(s) 451, 556, 824
LguI GCTCTTC 1 cut(s) 423
LmnI GCTCC 1 cut(s) 412
Lsp1109I GCAGC 6 cut(s) 152, 155, 276, 416, 635, 764
LweI GCATC 1 cut(s) 391
MaeI CTAG 5 cut(s) 360, 476, 497, 770, 888
MaeIII GTNAC 1 cut(s) 269
MalI GATC 3 cut(s) 453, 558, 826
MboI GATC 3 cut(s) 451, 556, 824
MboII GAAGA 5 cut(s) 206, 251, 440, 491, 655
MfeI CAATTG 1 cut(s) 128
MhlI GDGCHC 3 cut(s) 44, 394, 936
MluCI AATT 5 cut(s) 123, 128, 420, 724, 940
MlyI GAGTC 1 cut(s) 752
MnlI CCTC 8 cut(s) 184, 272, 391, 457, 548, 572, 627, 755
MroXI GAANNNNTTC 1 cut(s) 877
MseI TTAA 3 cut(s) 222, 785, 798
MspA1I CMGCKG 1 cut(s) 562
MspCI CTTAAG 1 cut(s) 221
MspR9I CCNGG 3 cut(s) 85, 395, 554
MunI CAATTG 1 cut(s) 128
MvaI CCWGG 3 cut(s) 85, 395, 554
MvnI CGCG 1 cut(s) 69
MwoI GCNNNNNNNGC 3 cut(s) 85, 264, 931
NdeII GATC 3 cut(s) 451, 556, 824
NlaIII CATG 3 cut(s) 644, 759, 814
NlaIV GGNNCC 2 cut(s) 392, 951
NruI TCGCGA 1 cut(s) 69
NspI RCATGY 1 cut(s) 644
PaeI GCATGC 1 cut(s) 644
PaqCI CACCTGC 1 cut(s) 175
PciSI GCTCTTC 1 cut(s) 423
PdmI GAANNNNTTC 1 cut(s) 877
PfeI GAWTC 2 cut(s) 692, 701
PflFI GACNNNGTC 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 552
PkrI GCNGC 6 cut(s) 142, 145, 266, 406, 625, 754
PleI GAGTC 1 cut(s) 751
PpsI GAGTC 1 cut(s) 751
Psp124BI GAGCTC 1 cut(s) 44
Psp6I CCWGG 3 cut(s) 83, 393, 552
PspGI CCWGG 3 cut(s) 83, 393, 552
PspN4I GGNNCC 2 cut(s) 392, 951
PspOMI GGGCCC 1 cut(s) 390
PspPI GGNCC 4 cut(s) 390, 391, 565, 949
PsyI GACNNNGTC 1 cut(s) 31
PvuII CAGCTG 1 cut(s) 562
RruI TCGCGA 1 cut(s) 69
RsaI GTAC 1 cut(s) 857
RsaNI GTAC 1 cut(s) 856
SacI GAGCTC 1 cut(s) 44
SapI GCTCTTC 1 cut(s) 423
SaqAI TTAA 3 cut(s) 222, 785, 798
SatI GCNGC 6 cut(s) 141, 144, 265, 405, 624, 753
Sau3AI GATC 3 cut(s) 451, 556, 824
Sau96I GGNCC 4 cut(s) 390, 391, 565, 949
SchI GAGTC 1 cut(s) 752
ScrFI CCNGG 3 cut(s) 85, 395, 554
SduI GDGCHC 3 cut(s) 44, 394, 936
SfaNI GCATC 1 cut(s) 391
SfcI CTRYAG 1 cut(s) 259
SinI GGWCC 1 cut(s) 949
SmlI CTYRAG 1 cut(s) 221
SmoI CTYRAG 1 cut(s) 221
SphI GCATGC 1 cut(s) 644
Sse9I AATT 5 cut(s) 123, 128, 420, 724, 940
SspMI CTAG 5 cut(s) 360, 476, 497, 770, 888
SstI GAGCTC 1 cut(s) 44
StyD4I CCNGG 3 cut(s) 83, 393, 552
StyI CCWWGG 1 cut(s) 496
TaaI ACNGT 1 cut(s) 731
TaqI TCGA 4 cut(s) 205, 454, 610, 684
TasI AATT 5 cut(s) 123, 128, 420, 724, 940
TatI WGTACW 1 cut(s) 855
TfiI GAWTC 2 cut(s) 692, 701
Tru1I TTAA 3 cut(s) 222, 785, 798
Tru9I TTAA 3 cut(s) 222, 785, 798
TscAI CASTG 1 cut(s) 797
TseI GCWGC 6 cut(s) 140, 143, 264, 404, 623, 752
TspDTI ATGAA 2 cut(s) 87, 714
TspRI CASTG 1 cut(s) 797
Tth111I GACNNNGTC 1 cut(s) 31
Vha464I CTTAAG 1 cut(s) 221
VpaK11BI GGWCC 1 cut(s) 949
XapI RAATTY 2 cut(s) 123, 420
XceI RCATGY 1 cut(s) 644
XmaJI CCTAGG 1 cut(s) 496
XmiI GTMKAC 1 cut(s) 816
XmnI GAANNNNTTC 1 cut(s) 877
XspI CTAG 5 cut(s) 360, 476, 497, 770, 888
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.