MD11G1300200.v1.1

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002523
Physical Location & Seq
Forward (+)
41629055 .. 41631072
2018 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1300200.v1.1.491

Sequence Viewer

Length: 930 bp
ATGTCAGACAAAGCTTCTGAGGAAACGCAGGAAACTCGCGATGAAATGCTTTCTAGGCACAGGAAAGAGATATCAAAACTTCAGGACAAGGAAACTGGAATGAAAAAGGCAGCTGCCAAAGGGAGCAAGGCTGAACAAAAAGCTAAGAAAAAGCAGGTGGAGGAACAGATTACTCGGCTTTCTGCTAATCTCAAAGAAAAACAGGCTGCAGAACTTGCTTCCTTAGGCTATAGCGCTAGTAACGGAAATGAGAAGAGCAAACTCGACACTTTAGTGAAGGCCATAGCTGGTGTTTCTGTCACCAGTCAACCTGAACATGCAAAGCCCAGCAAGAGCTCAAAGAGGCGAGAAAAGAGGGCTCAGCAAGATGCAGCTAGGGAGCAAAGAATTCAAGAAGAGCAGAGCAACCTAGTAAGTGATCGAGTGATTGAGGATGAGAAACTAGAGAAAAAGCTCGAACCCTTTGGCTTGATCATTAAAGAAATAAAGCCCGATGGACATTGCCTCTACCGAGCTATCCAGGATCAGCTGGCTCACCTTTCAGGAGGTTCATCACCTTACACGTACCAACAGCTTCGAGAAATGGTGGCTGCTTATATGAGGAAGCATGCATCTGATTTTCTCCCTTTTTTCTTATCGGAGAATCCGGCAGATGGAGATTCTGATGATTCACTTGCAGAGAGGTTTGAGAACTACTGTAAAGAAGTGGAGTCAACAGCAGCATGGGGAGGACAGCTAGAGCTTGGTGCTTTAACTCACTGCCTCAAAAAGCCTATCAAGATATACTCAGGATCCTTCCCTGATGTGGAGATGGGCAAGGAGTACAAATCCGATAGCGCGACTGGTTCCTCCGATTCGCGGATTATGTTGTCATACCATAAGCATGCATTTGGGCTTGGTGAGCACTATAATTCCGTAGTTCCAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

310

Amino Acids

34.52

Weight (kDa)

7.13

Isoelectric Point (pI)

44.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 163 - 303 5e-35 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 145
Acc36I ACCTGC 1 cut(s) 145
AccII CGCG 3 cut(s) 39, 839, 859
AciI CCGC 1 cut(s) 859
AclWI GGATC 3 cut(s) 531, 786, 799
AcsI RAATTY 1 cut(s) 387
AcuI CTGAAG 1 cut(s) 65
AfaI GTAC 2 cut(s) 566, 824
AfeI AGCGCT 1 cut(s) 235
AfiI CCNNNNNNNGG 3 cut(s) 653, 805, 858
AflIII ACRYGT 1 cut(s) 561
AgsI TTSAA 1 cut(s) 392
AjnI CCWGG 1 cut(s) 519
AleI CACNNNNGTG 1 cut(s) 272
Alw21I GWGCWC 2 cut(s) 338, 906
AlwI GGATC 3 cut(s) 531, 786, 799
Aor51HI AGCGCT 1 cut(s) 235
AoxI GGCC 1 cut(s) 279
ApeKI GCWGC 6 cut(s) 110, 113, 206, 371, 590, 719
ApoI RAATTY 1 cut(s) 387
AspLEI GCGC 2 cut(s) 236, 839
AsuHPI GGTGA 4 cut(s) 292, 527, 546, 911
AxyI CCTNAGG 1 cut(s) 223
BamHI GGATCC 1 cut(s) 791
BanII GRGCYC 2 cut(s) 338, 361
Bbv12I GWGCWC 2 cut(s) 338, 906
BbvI GCAGC 6 cut(s) 100, 122, 193, 383, 577, 731
BccI CCATC 3 cut(s) 488, 647, 805
BcgI CGANNNNNNTGC 2 cut(s) 17, 51
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 1 cut(s) 471
BfaI CTAG 6 cut(s) 54, 237, 375, 410, 443, 737
BfmI CTRYAG 2 cut(s) 207, 229
BfoI RGCGCY 1 cut(s) 237
BfuAI ACCTGC 1 cut(s) 145
BisI GCNGC 6 cut(s) 111, 114, 207, 372, 591, 720
BlpI GCTNAGC 1 cut(s) 360
BlsI GCNGC 6 cut(s) 112, 115, 208, 373, 592, 721
Bme1390I CCNGG 1 cut(s) 521
BmiI GGNNCC 2 cut(s) 793, 847
BmrFI CCNGG 1 cut(s) 521
BmsI GCATC 2 cut(s) 358, 620
Bpu1102I GCTNAGC 1 cut(s) 360
BsaAI YACGTR 1 cut(s) 564
Bsc4I CCNNNNNNNGG 3 cut(s) 653, 805, 858
Bse1I ACTGG 3 cut(s) 100, 303, 847
Bse21I CCTNAGG 1 cut(s) 223
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 1 cut(s) 521
BseGI GGATG 1 cut(s) 439
BseLI CCNNNNNNNGG 3 cut(s) 653, 805, 858
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 3 cut(s) 9, 374, 801
BseNI ACTGG 3 cut(s) 100, 303, 847
BseXI GCAGC 6 cut(s) 100, 122, 193, 383, 577, 731
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 3 cut(s) 39, 839, 859
BshFI GGCC 1 cut(s) 281
BsiHKAI GWGCWC 2 cut(s) 338, 906
BsiSI CCGG 1 cut(s) 647
BslI CCNNNNNNNGG 3 cut(s) 653, 805, 858
BsnI GGCC 1 cut(s) 281
Bsp1286I GDGCHC 3 cut(s) 338, 361, 906
Bsp143I GATC 4 cut(s) 418, 471, 523, 791
Bsp1720I GCTNAGC 1 cut(s) 360
Bsp68I TCGCGA 1 cut(s) 39
BspACI CCGC 1 cut(s) 859
BspANI GGCC 1 cut(s) 281
BspCNI CTCAG 3 cut(s) 10, 373, 800
BspFNI CGCG 3 cut(s) 39, 839, 859
BspLI GGNNCC 2 cut(s) 793, 847
BspMAI CTGCAG 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 3 cut(s) 531, 786, 799
BspQI GCTCTTC 2 cut(s) 248, 390
BsrDI GCAATG 1 cut(s) 499
BsrI ACTGG 3 cut(s) 100, 303, 847
BssMI GATC 4 cut(s) 418, 471, 523, 791
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 1 cut(s) 698
Bst6I CTCTTC 2 cut(s) 248, 390
BstAPI GCANNNNNTGC 1 cut(s) 215
BstBAI YACGTR 1 cut(s) 564
BstC8I GCNNGC 3 cut(s) 531, 609, 885
BstDEI CTNAG 5 cut(s) 18, 144, 223, 360, 787
BstF5I GGATG 1 cut(s) 439
BstFNI CGCG 3 cut(s) 39, 839, 859
BstH2I RGCGCY 1 cut(s) 237
BstHHI GCGC 2 cut(s) 236, 839
BstKTI GATC 4 cut(s) 421, 474, 526, 794
BstMBI GATC 4 cut(s) 418, 471, 523, 791
BstMWI GCNNNNNNNGC 3 cut(s) 55, 215, 901
BstNI CCWGG 1 cut(s) 521
BstNSI RCATGY 3 cut(s) 320, 611, 887
BstSCI CCNGG 1 cut(s) 519
BstSFI CTRYAG 2 cut(s) 207, 229
BstUI CGCG 3 cut(s) 39, 839, 859
BstV1I GCAGC 6 cut(s) 100, 122, 193, 383, 577, 731
BstX2I RGATCY 1 cut(s) 791
BstYI RGATCY 1 cut(s) 791
Bsu36I CCTNAGG 1 cut(s) 223
BsuRI GGCC 1 cut(s) 281
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 439
BtsI GCAGTG 1 cut(s) 757
BtsIMutI CAGTG 1 cut(s) 757
BtuMI TCGCGA 1 cut(s) 39
BveI ACCTGC 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 531, 609, 885
CfoI GCGC 2 cut(s) 236, 839
Csp6I GTAC 2 cut(s) 565, 823
CviAII CATG 4 cut(s) 317, 608, 723, 884
CviQI GTAC 2 cut(s) 565, 823
DdeI CTNAG 5 cut(s) 18, 144, 223, 360, 787
DpnI GATC 4 cut(s) 420, 473, 525, 793
DpnII GATC 4 cut(s) 418, 471, 523, 791
Eam1104I CTCTTC 2 cut(s) 248, 390
EarI CTCTTC 2 cut(s) 248, 390
Ecl136II GAGCTC 1 cut(s) 336
Eco24I GRGCYC 2 cut(s) 338, 361
Eco32I GATATC 1 cut(s) 72
Eco47III AGCGCT 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 336
Eco57I CTGAAG 1 cut(s) 65
Eco81I CCTNAGG 1 cut(s) 223
EcoICRI GAGCTC 1 cut(s) 336
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 1 cut(s) 519
EcoRV GATATC 1 cut(s) 72
EcoT22I ATGCAT 2 cut(s) 613, 889
EcoT38I GRGCYC 2 cut(s) 338, 361
FaeI CATG 4 cut(s) 320, 611, 726, 887
FatI CATG 4 cut(s) 316, 607, 722, 883
FbaI TGATCA 1 cut(s) 471
Fnu4HI GCNGC 6 cut(s) 111, 114, 207, 372, 591, 720
FokI GGATG 1 cut(s) 446
FriOI GRGCYC 2 cut(s) 338, 361
Fsp4HI GCNGC 6 cut(s) 111, 114, 207, 372, 591, 720
FspBI CTAG 6 cut(s) 54, 237, 375, 410, 443, 737
GlaI GCGC 2 cut(s) 235, 838
GluI GCNGC 6 cut(s) 111, 114, 207, 372, 591, 720
GsaI CCCAGC 1 cut(s) 330
HaeII RGCGCY 1 cut(s) 237
HaeIII GGCC 1 cut(s) 281
HapII CCGG 1 cut(s) 647
HhaI GCGC 2 cut(s) 236, 839
Hin1II CATG 4 cut(s) 320, 611, 726, 887
Hin6I GCGC 2 cut(s) 234, 837
HinP1I GCGC 2 cut(s) 234, 837
HincII GTYRAC 2 cut(s) 308, 714
HindII GTYRAC 2 cut(s) 308, 714
HindIII AAGCTT 1 cut(s) 12
HinfI GANTC 5 cut(s) 643, 659, 668, 710, 854
HpaII CCGG 1 cut(s) 647
HphI GGTGA 4 cut(s) 292, 527, 546, 911
Hpy166II GTNNAC 2 cut(s) 308, 714
Hpy188I TCNGA 7 cut(s) 7, 19, 616, 640, 664, 832, 853
Hpy188III TCNNGA 7 cut(s) 38, 83, 392, 543, 578, 778, 789
Hpy8I GTNNAC 2 cut(s) 308, 714
HpyAV CCTTC 2 cut(s) 271, 805
HpyCH4III ACNGT 1 cut(s) 698
HpyCH4IV ACGT 1 cut(s) 563
HpyCH4V TGCA 6 cut(s) 209, 320, 371, 611, 677, 887
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 215, 901
HpyF3I CTNAG 5 cut(s) 18, 144, 223, 360, 787
HpySE526I ACGT 1 cut(s) 563
Hsp92II CATG 4 cut(s) 320, 611, 726, 887
HspAI GCGC 2 cut(s) 234, 837
Ksp22I TGATCA 1 cut(s) 471
Kzo9I GATC 4 cut(s) 418, 471, 523, 791
LguI GCTCTTC 2 cut(s) 248, 390
LmnI GCTCC 2 cut(s) 123, 379
Lsp1109I GCAGC 6 cut(s) 100, 122, 193, 383, 577, 731
LweI GCATC 2 cut(s) 358, 620
MaeI CTAG 6 cut(s) 54, 237, 375, 410, 443, 737
MaeII ACGT 1 cut(s) 563
MaeIII GTNAC 2 cut(s) 239, 298
MalI GATC 4 cut(s) 420, 473, 525, 793
MboI GATC 4 cut(s) 418, 471, 523, 791
MboII GAAGA 2 cut(s) 265, 407
MflI RGATCY 1 cut(s) 791
MhlI GDGCHC 3 cut(s) 338, 361, 906
MluCI AATT 2 cut(s) 387, 910
MlyI GAGTC 1 cut(s) 719
Mph1103I ATGCAT 2 cut(s) 613, 889
MseI TTAA 2 cut(s) 477, 752
MslI CAYNNNNRTG 2 cut(s) 272, 882
MspA1I CMGCKG 2 cut(s) 113, 529
MspI CCGG 1 cut(s) 647
MspR9I CCNGG 1 cut(s) 521
MvaI CCWGG 1 cut(s) 521
MvnI CGCG 3 cut(s) 39, 839, 859
MwoI GCNNNNNNNGC 3 cut(s) 55, 215, 901
NdeII GATC 4 cut(s) 418, 471, 523, 791
NlaIII CATG 4 cut(s) 320, 611, 726, 887
NlaIV GGNNCC 2 cut(s) 793, 847
NmeAIII GCCGAG 1 cut(s) 154
NmuCI GTSAC 1 cut(s) 298
NruI TCGCGA 1 cut(s) 39
NsiI ATGCAT 2 cut(s) 613, 889
NspI RCATGY 3 cut(s) 320, 611, 887
OliI CACNNNNGTG 1 cut(s) 272
PaeI GCATGC 2 cut(s) 611, 887
PaqCI CACCTGC 1 cut(s) 145
PciSI GCTCTTC 2 cut(s) 248, 390
PfeI GAWTC 4 cut(s) 643, 659, 668, 854
PfoI TCCNGGA 1 cut(s) 519
PkrI GCNGC 6 cut(s) 112, 115, 208, 373, 592, 721
PleI GAGTC 1 cut(s) 718
PpsI GAGTC 1 cut(s) 718
Ppu21I YACGTR 1 cut(s) 564
Psp124BI GAGCTC 1 cut(s) 338
Psp6I CCWGG 1 cut(s) 519
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 2 cut(s) 793, 847
PstI CTGCAG 1 cut(s) 211
PsuI RGATCY 1 cut(s) 791
PvuII CAGCTG 2 cut(s) 113, 529
RruI TCGCGA 1 cut(s) 39
RsaI GTAC 2 cut(s) 566, 824
RsaNI GTAC 2 cut(s) 565, 823
RseI CAYNNNNRTG 2 cut(s) 272, 882
SacI GAGCTC 1 cut(s) 338
SapI GCTCTTC 2 cut(s) 248, 390
SaqAI TTAA 2 cut(s) 477, 752
SatI GCNGC 6 cut(s) 111, 114, 207, 372, 591, 720
Sau3AI GATC 4 cut(s) 418, 471, 523, 791
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 521
SduI GDGCHC 3 cut(s) 338, 361, 906
SfaNI GCATC 2 cut(s) 358, 620
SfcI CTRYAG 2 cut(s) 207, 229
SmiMI CAYNNNNRTG 2 cut(s) 272, 882
SphI GCATGC 2 cut(s) 611, 887
Sse9I AATT 2 cut(s) 387, 910
SsiI CCGC 1 cut(s) 859
SspMI CTAG 6 cut(s) 54, 237, 375, 410, 443, 737
SstI GAGCTC 1 cut(s) 338
StyD4I CCNGG 1 cut(s) 519
TaaI ACNGT 1 cut(s) 698
TaiI ACGT 1 cut(s) 566
TaqI TCGA 4 cut(s) 264, 421, 456, 577
TasI AATT 2 cut(s) 387, 910
TatI WGTACW 1 cut(s) 822
TfiI GAWTC 4 cut(s) 643, 659, 668, 854
Tru1I TTAA 2 cut(s) 477, 752
Tru9I TTAA 2 cut(s) 477, 752
TscAI CASTG 1 cut(s) 764
TseFI GTSAC 1 cut(s) 298
TseI GCWGC 6 cut(s) 110, 113, 206, 371, 590, 719
Tsp45I GTSAC 1 cut(s) 298
TspDTI ATGAA 3 cut(s) 57, 116, 540
TspGWI ACGGA 2 cut(s) 258, 904
TspRI CASTG 1 cut(s) 764
XapI RAATTY 1 cut(s) 387
XceI RCATGY 3 cut(s) 320, 611, 887
XspI CTAG 6 cut(s) 54, 237, 375, 410, 443, 737
Zsp2I ATGCAT 2 cut(s) 613, 889
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.