pycom03g22160

OTU domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002523
Physical Location & Seq
Forward (+)
22317948 .. 22319738
1791 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g22160.1

Sequence Viewer

Length: 1008 bp
ATGATCGCCGTCGACGGCAAAGGAGGACTCTCAGGCAGGGTTGTTTCAATGGAGGATGCACAAGAAGTATCTGATACAATGTCAGACAAAGCTTCTGAGAATACACAGGAAACTCGTGACGAAATGCTTTCTAGGCACAGGAAAGAGATATCAAAACTTCAGGACAAGGAAACTGAAATGAAAAAGGCAGCTGCCAAAGGGAGCAAGGCTGAACAAAAAGCTAAGAAAAAGCAGGTGGAGGAAGAGATTACTCGGCTTTCTGCTAATCTTAAAGAAAAACATGCTGCAGAACTTGTTTCGTTAGGCTATAGTGCTAGTAATGAGAATGAGAAAAGTAAACTTGACACTTTAGTGAAGGCCATAGCTGGTGTTTCTGTCACCAGTCAACCTGAGCAGGCAAAGCCCAGCAAGAGTTCGAAGAGGCGAGAGAAGAGGGCTCAGCAAGATGCAGCCAGGGAGCAAAGAATTCAAGAAGAGCAGAGCAACTTAGTAAGTGATCGAATGATTGAGGGTGAAAAACTAGAGAAAAAGCTCGAACCCTTTGGCTTGACCATTAATGAAATAAAGCCTGATGGCCATTGCCTCTACCGAGCTATCCAGGATCAGCTGACCCACCTTTCCGGAGGTTCATCACCTTACACGTACCAACAGCTTCGAGAAATGGTAGCTGCTTATATGAGGAAGCATGCATCTGATTTTCTCCCTTTTTTCTTGTCGGAGAATCCAGCAGATGGAGATTCTGACGAGTCACTTGCAGAGAGGTTTGAGAACTACTGTAAAGAAGTGGAGTCAACTGCAGCATGGGGAGGACAGCTAGAGCTCGGTGCTTTAACGCACTGCCTTAAAAAGCCTATCAAGATATACTCAGGATCCTTCCCTGATGTGGAGATGGGGAAGGAGTACAAATCCGATAGCGGTACAGGTTCTTCCGATTCGAGTATTATGTTGTCATACCATAAGCATGCATTTGGGCTTGGTGAGCACTATAATTCCGTGGTTCGAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

336

Amino Acids

37.28

Weight (kDa)

6.07

Isoelectric Point (pI)

41.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 189 - 329 5.2e-35 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 223
Acc36I ACCTGC 1 cut(s) 223
AccB7I CCANNNNNTGG 1 cut(s) 731
AccI GTMKAC 1 cut(s) 12
AccIII TCCGGA 1 cut(s) 620
AciI CCGC 1 cut(s) 915
AclWI GGATC 3 cut(s) 609, 864, 877
AcoI YGGCCR 1 cut(s) 574
AcsI RAATTY 1 cut(s) 465
AcuI CTGAAG 1 cut(s) 143
AfaI GTAC 3 cut(s) 644, 902, 919
AfiI CCNNNNNNNGG 2 cut(s) 731, 883
AflIII ACRYGT 1 cut(s) 639
AgsI TTSAA 2 cut(s) 48, 470
AjnI CCWGG 2 cut(s) 452, 597
AleI CACNNNNGTG 1 cut(s) 350
Alw21I GWGCWC 2 cut(s) 822, 984
AlwI GGATC 3 cut(s) 609, 864, 877
Aor13HI TCCGGA 1 cut(s) 620
AoxI GGCC 2 cut(s) 357, 574
ApeKI GCWGC 6 cut(s) 188, 191, 284, 449, 668, 797
ApoI RAATTY 1 cut(s) 465
AseI ATTAAT 1 cut(s) 555
AsuHPI GGTGA 4 cut(s) 370, 524, 624, 989
AsuII TTCGAA 2 cut(s) 416, 1000
BalI TGGCCA 1 cut(s) 576
BamHI GGATCC 1 cut(s) 869
BanII GRGCYC 2 cut(s) 439, 822
BarI GAAGNNNNNNTAC 2 cut(s) 910, 942
BauI CACGAG 1 cut(s) 114
Bbv12I GWGCWC 2 cut(s) 822, 984
BbvI GCAGC 6 cut(s) 178, 200, 271, 461, 655, 809
BccI CCATC 3 cut(s) 566, 725, 883
BceAI ACGGC 1 cut(s) 31
BcgI CGANNNNNNTGC 2 cut(s) 734, 768
BciT130I CCWGG 2 cut(s) 454, 599
BfaI CTAG 4 cut(s) 132, 315, 521, 815
BfmI CTRYAG 3 cut(s) 285, 307, 795
BfuAI ACCTGC 1 cut(s) 223
BisI GCNGC 6 cut(s) 189, 192, 285, 450, 669, 798
BlpI GCTNAGC 1 cut(s) 438
BlsI GCNGC 6 cut(s) 190, 193, 286, 451, 670, 799
Bme1390I CCNGG 2 cut(s) 454, 599
BmiI GGNNCC 1 cut(s) 871
BmrFI CCNGG 2 cut(s) 454, 599
BmsI GCATC 3 cut(s) 46, 436, 698
Bpu10I CCTNAGC 1 cut(s) 390
Bpu1102I GCTNAGC 1 cut(s) 438
Bpu14I TTCGAA 2 cut(s) 416, 1000
BsaAI YACGTR 1 cut(s) 642
BsaJI CCNNGG 2 cut(s) 453, 993
BsaWI WCCGGW 1 cut(s) 620
Bsc4I CCNNNNNNNGG 2 cut(s) 731, 883
Bse1I ACTGG 1 cut(s) 381
Bse3DI GCAATG 1 cut(s) 577
BseAI TCCGGA 1 cut(s) 620
BseBI CCWGG 2 cut(s) 454, 599
BseDI CCNNGG 2 cut(s) 453, 993
BseGI GGATG 1 cut(s) 61
BseLI CCNNNNNNNGG 2 cut(s) 731, 883
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 5 cut(s) 45, 87, 381, 452, 879
BseNI ACTGG 1 cut(s) 381
BseXI GCAGC 6 cut(s) 178, 200, 271, 461, 655, 809
BseYI CCCAGC 1 cut(s) 404
BshFI GGCC 2 cut(s) 359, 576
BsiHKAI GWGCWC 2 cut(s) 822, 984
BsiSI CCGG 1 cut(s) 621
BslI CCNNNNNNNGG 2 cut(s) 731, 883
BsnI GGCC 2 cut(s) 359, 576
Bsp119I TTCGAA 2 cut(s) 416, 1000
Bsp1286I GDGCHC 3 cut(s) 439, 822, 984
Bsp13I TCCGGA 1 cut(s) 620
Bsp143I GATC 4 cut(s) 3, 496, 601, 869
Bsp1720I GCTNAGC 1 cut(s) 438
BspACI CCGC 1 cut(s) 915
BspANI GGCC 2 cut(s) 359, 576
BspCNI CTCAG 5 cut(s) 44, 88, 382, 451, 878
BspEI TCCGGA 1 cut(s) 620
BspLI GGNNCC 1 cut(s) 871
BspMAI CTGCAG 2 cut(s) 289, 799
BspMI ACCTGC 1 cut(s) 223
BspPI GGATC 3 cut(s) 609, 864, 877
BspQI GCTCTTC 1 cut(s) 468
BspT104I TTCGAA 2 cut(s) 416, 1000
BsrDI GCAATG 1 cut(s) 577
BsrI ACTGG 1 cut(s) 381
BssECI CCNNGG 2 cut(s) 453, 993
BssMI GATC 4 cut(s) 3, 496, 601, 869
BssSI CACGAG 1 cut(s) 114
Bst2BI CACGAG 1 cut(s) 114
Bst2UI CCWGG 2 cut(s) 454, 599
Bst4CI ACNGT 1 cut(s) 776
Bst6I CTCTTC 4 cut(s) 237, 413, 425, 468
BstBAI YACGTR 1 cut(s) 642
BstBI TTCGAA 2 cut(s) 416, 1000
BstC8I GCNNGC 3 cut(s) 396, 687, 963
BstDEI CTNAG 7 cut(s) 31, 96, 222, 390, 438, 487, 865
BstDSI CCRYGG 1 cut(s) 993
BstF5I GGATG 1 cut(s) 61
BstKTI GATC 4 cut(s) 6, 499, 604, 872
BstMBI GATC 4 cut(s) 3, 496, 601, 869
BstMWI GCNNNNNNNGC 3 cut(s) 133, 400, 979
BstNI CCWGG 2 cut(s) 454, 599
BstNSI RCATGY 3 cut(s) 284, 689, 965
BstSCI CCNGG 2 cut(s) 452, 597
BstSFI CTRYAG 3 cut(s) 285, 307, 795
BstV1I GCAGC 6 cut(s) 178, 200, 271, 461, 655, 809
BstX2I RGATCY 1 cut(s) 869
BstYI RGATCY 1 cut(s) 869
BsuRI GGCC 2 cut(s) 359, 576
BtgI CCRYGG 1 cut(s) 993
BtsCI GGATG 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 835
BtsIMutI CAGTG 1 cut(s) 835
BveI ACCTGC 1 cut(s) 223
Cac8I GCNNGC 3 cut(s) 396, 687, 963
Csp6I GTAC 3 cut(s) 643, 901, 918
CviAII CATG 4 cut(s) 281, 686, 801, 962
CviQI GTAC 3 cut(s) 643, 901, 918
DdeI CTNAG 7 cut(s) 31, 96, 222, 390, 438, 487, 865
DpnI GATC 4 cut(s) 5, 498, 603, 871
DpnII GATC 4 cut(s) 3, 496, 601, 869
EaeI YGGCCR 1 cut(s) 574
Eam1104I CTCTTC 4 cut(s) 237, 413, 425, 468
EarI CTCTTC 4 cut(s) 237, 413, 425, 468
Ecl136II GAGCTC 1 cut(s) 820
Eco24I GRGCYC 2 cut(s) 439, 822
Eco32I GATATC 1 cut(s) 150
Eco53kI GAGCTC 1 cut(s) 820
Eco57I CTGAAG 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 820
EcoRI GAATTC 1 cut(s) 465
EcoRII CCWGG 2 cut(s) 452, 597
EcoRV GATATC 1 cut(s) 150
EcoT22I ATGCAT 2 cut(s) 691, 967
EcoT38I GRGCYC 2 cut(s) 439, 822
FaeI CATG 4 cut(s) 284, 689, 804, 965
FatI CATG 4 cut(s) 280, 685, 800, 961
FblI GTMKAC 1 cut(s) 12
Fnu4HI GCNGC 6 cut(s) 189, 192, 285, 450, 669, 798
FokI GGATG 1 cut(s) 68
FriOI GRGCYC 2 cut(s) 439, 822
Fsp4HI GCNGC 6 cut(s) 189, 192, 285, 450, 669, 798
FspBI CTAG 4 cut(s) 132, 315, 521, 815
GluI GCNGC 6 cut(s) 189, 192, 285, 450, 669, 798
GsaI CCCAGC 1 cut(s) 408
HaeIII GGCC 2 cut(s) 359, 576
HapII CCGG 1 cut(s) 621
Hin1II CATG 4 cut(s) 284, 689, 804, 965
HincII GTYRAC 3 cut(s) 13, 386, 792
HindII GTYRAC 3 cut(s) 13, 386, 792
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 6 cut(s) 27, 721, 737, 746, 788, 932
HpaII CCGG 1 cut(s) 621
HphI GGTGA 4 cut(s) 370, 524, 624, 989
Hpy166II GTNNAC 4 cut(s) 13, 338, 386, 792
Hpy188I TCNGA 8 cut(s) 73, 85, 97, 694, 718, 742, 910, 931
Hpy188III TCNNGA 7 cut(s) 116, 161, 470, 621, 656, 856, 867
Hpy8I GTNNAC 4 cut(s) 13, 338, 386, 792
Hpy99I CGWCG 2 cut(s) 14, 17
HpyAV CCTTC 3 cut(s) 349, 883, 889
HpyCH4III ACNGT 1 cut(s) 776
HpyCH4IV ACGT 1 cut(s) 641
HpyCH4V TGCA 7 cut(s) 59, 287, 449, 689, 755, 797, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 400, 979
HpyF3I CTNAG 7 cut(s) 31, 96, 222, 390, 438, 487, 865
HpySE526I ACGT 1 cut(s) 641
Hsp92II CATG 4 cut(s) 284, 689, 804, 965
Kpn2I TCCGGA 1 cut(s) 620
Kzo9I GATC 4 cut(s) 3, 496, 601, 869
LguI GCTCTTC 1 cut(s) 468
LmnI GCTCC 2 cut(s) 201, 457
Lsp1109I GCAGC 6 cut(s) 178, 200, 271, 461, 655, 809
LweI GCATC 3 cut(s) 46, 436, 698
MaeI CTAG 4 cut(s) 132, 315, 521, 815
MaeII ACGT 1 cut(s) 641
MaeIII GTNAC 3 cut(s) 116, 376, 747
MalI GATC 4 cut(s) 5, 498, 603, 871
MboI GATC 4 cut(s) 3, 496, 601, 869
MboII GAAGA 5 cut(s) 254, 430, 442, 485, 918
MflI RGATCY 1 cut(s) 869
MhlI GDGCHC 3 cut(s) 439, 822, 984
MlsI TGGCCA 1 cut(s) 576
MluCI AATT 2 cut(s) 465, 988
MluNI TGGCCA 1 cut(s) 576
MlyI GAGTC 3 cut(s) 21, 755, 797
MmeI TCCRAC 1 cut(s) 696
Mox20I TGGCCA 1 cut(s) 576
Mph1103I ATGCAT 2 cut(s) 691, 967
MroI TCCGGA 1 cut(s) 620
MscI TGGCCA 1 cut(s) 576
MseI TTAA 4 cut(s) 270, 555, 830, 843
MslI CAYNNNNRTG 2 cut(s) 350, 960
Msp20I TGGCCA 1 cut(s) 576
MspA1I CMGCKG 2 cut(s) 191, 607
MspI CCGG 1 cut(s) 621
MspR9I CCNGG 2 cut(s) 454, 599
MvaI CCWGG 2 cut(s) 454, 599
MwoI GCNNNNNNNGC 3 cut(s) 133, 400, 979
NdeII GATC 4 cut(s) 3, 496, 601, 869
NlaIII CATG 4 cut(s) 284, 689, 804, 965
NlaIV GGNNCC 1 cut(s) 871
NmeAIII GCCGAG 1 cut(s) 232
NmuCI GTSAC 3 cut(s) 116, 376, 747
NsiI ATGCAT 2 cut(s) 691, 967
NspI RCATGY 3 cut(s) 284, 689, 965
NspV TTCGAA 2 cut(s) 416, 1000
OliI CACNNNNGTG 1 cut(s) 350
PaeI GCATGC 2 cut(s) 689, 965
PaqCI CACCTGC 1 cut(s) 223
PciSI GCTCTTC 1 cut(s) 468
PfeI GAWTC 3 cut(s) 721, 737, 932
PflMI CCANNNNNTGG 1 cut(s) 731
PfoI TCCNGGA 1 cut(s) 597
PkrI GCNGC 6 cut(s) 190, 193, 286, 451, 670, 799
PleI GAGTC 3 cut(s) 21, 754, 796
PpsI GAGTC 3 cut(s) 21, 754, 796
Ppu21I YACGTR 1 cut(s) 642
PshBI ATTAAT 1 cut(s) 555
Psp124BI GAGCTC 1 cut(s) 822
Psp6I CCWGG 2 cut(s) 452, 597
PspFI CCCAGC 1 cut(s) 404
PspGI CCWGG 2 cut(s) 452, 597
PspN4I GGNNCC 1 cut(s) 871
PstI CTGCAG 2 cut(s) 289, 799
PsuI RGATCY 1 cut(s) 869
PvuII CAGCTG 2 cut(s) 191, 607
RsaI GTAC 3 cut(s) 644, 902, 919
RsaNI GTAC 3 cut(s) 643, 901, 918
RseI CAYNNNNRTG 2 cut(s) 350, 960
SacI GAGCTC 1 cut(s) 822
SalI GTCGAC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 4 cut(s) 270, 555, 830, 843
SatI GCNGC 6 cut(s) 189, 192, 285, 450, 669, 798
Sau3AI GATC 4 cut(s) 3, 496, 601, 869
SchI GAGTC 3 cut(s) 21, 755, 797
ScrFI CCNGG 2 cut(s) 454, 599
SduI GDGCHC 3 cut(s) 439, 822, 984
SfaNI GCATC 3 cut(s) 46, 436, 698
SfcI CTRYAG 3 cut(s) 285, 307, 795
SfuI TTCGAA 2 cut(s) 416, 1000
SgrDI CGTCGACG 1 cut(s) 11
SmiMI CAYNNNNRTG 2 cut(s) 350, 960
SphI GCATGC 2 cut(s) 689, 965
Sse9I AATT 2 cut(s) 465, 988
SsiI CCGC 1 cut(s) 915
SspMI CTAG 4 cut(s) 132, 315, 521, 815
SstI GAGCTC 1 cut(s) 822
StyD4I CCNGG 2 cut(s) 452, 597
TaaI ACNGT 1 cut(s) 776
TaiI ACGT 1 cut(s) 644
TaqI TCGA 7 cut(s) 12, 416, 499, 534, 655, 935, 1000
TasI AATT 2 cut(s) 465, 988
TatI WGTACW 1 cut(s) 900
TfiI GAWTC 3 cut(s) 721, 737, 932
Tru1I TTAA 4 cut(s) 270, 555, 830, 843
Tru9I TTAA 4 cut(s) 270, 555, 830, 843
TscAI CASTG 1 cut(s) 842
TseFI GTSAC 3 cut(s) 116, 376, 747
TseI GCWGC 6 cut(s) 188, 191, 284, 449, 668, 797
Tsp45I GTSAC 3 cut(s) 116, 376, 747
TspDTI ATGAA 3 cut(s) 194, 573, 618
TspGWI ACGGA 1 cut(s) 982
TspRI CASTG 1 cut(s) 842
Van91I CCANNNNNTGG 1 cut(s) 731
VspI ATTAAT 1 cut(s) 555
XapI RAATTY 1 cut(s) 465
XceI RCATGY 3 cut(s) 284, 689, 965
XmiI GTMKAC 1 cut(s) 12
XspI CTAG 4 cut(s) 132, 315, 521, 815
Zsp2I ATGCAT 2 cut(s) 691, 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.