Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000967

Physical Location & Seq

Genomic Coordinates Reverse (-)

13663415 .. 13664621

Gene Structure 1207 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT4G27280.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 393 bp

ATGGCGTCACCAAAGTCACCCACAAGACCAACCCAACAAAACCCACAACCCAATTTCCACGATTTTCTTCCTACCATGGCCGGAAACCTAGGCGGTGAAGGTCTGATCGGAGAGCTATGCAACGGCTTCGAGCTTTTGATGGACAGAGAGAAAGGTGTCATCACGTTCGAGAGTCTCCGACGTAACGCTGCGGCGGTTCTGGGTTTAGGAGATTTGACTGATGAAGATGTCCGGTGTATGATTAAAGAAGGTGATTTTGACTGCGACGGTGCGTTGAATCAGATGGAGTTTTGTGTGTTGATGTTTAGGCTTAGCCCTGATTTGATGGAAGCGTCGCGGTGTCTCGTCACGGAGGTCATTGAGGAGGAGTTTGGCTTCACGCGCCGGCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005509 GO:0008150 GO:0009628 GO:0009719 GO:0009725 GO:0009733 GO:0010033 GO:0042221 GO:0043167 GO:0043169 GO:0046872 GO:0050896 GO:0080167

KEGG Pathways

Metabolic & Signaling

ko03420 map03420

Pfam Domains

Protein Families

EF-hand_8

Protein Analysis

130

Amino Acids

14.5

Weight (kDa)

4.54

Isoelectric Point (pI)

53.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
EF-hand_8	PF13833	50 - 103	4.1e-22	EF-hand domain pair

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Publication-ready

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Orthologous Genes (Group: OG0000967)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G27280 AT5G54490
fragaria_vesca	FvH4_3g03600 FvH4_3g03610 FvH4_3g21340 FvH4_6g44060
malus_domestica	MD03G1185700.v1.1 MD05G1329200.v1.1 MD05G1329500.v1.1 MD09G1101400.v1.1 MD10G1306100.v1.1 MD11G1233100.v1.1 MD17G1088900.v1.1
prunus_persica	Prupe.3G224800_v2.0.a1 Prupe.4G033300_v2.0.a1 Prupe.4G033400_v2.0.a1 Prupe.4G033500_v2.0.a1 Prupe.4G193800_v2.0.a1
pyrus_communis	pycom03g16550 pycom05g30250 pycom05g30260
rosa_chinensis	RchiOBHm_Chr2g0160761 RchiOBHm_Chr5g0005661 RchiOBHm_Chr5g0005671 RchiOBHm_Chr5g0036541
rosa_laevigata	RLG00000021240 RLG00000031315 RLG00000031316 RLG00000033704
rosa_multiflora	Rmu_sc0000149.1_g000045 Rmu_sc0000570.1_g000024 Rmu_sc0000570.1_g000026 Rmu_sc0002773.1_g000010 Rmu_sc0006059.1_g000036
rosa_roxburghii	Rroxscaffold_1G00044350 Rroxscaffold_1G00070170 Rroxscaffold_1G00070180 Rroxscaffold_2G00089390
rosa_rugosa	Rorug02G0485800 Rorug04G0417000 Rorug04G0417100 Rorug05G0157600
rosa_samantha	Rh2AG550800 Rh2CG535200 Rh2DG574300 Rh5AG044500 Rh5AG044700 Rh5AG249300 Rh5BG043400 Rh5BG043500 Rh5BG250500 Rh5CG052100 Rh5CG052300 Rh5CG282900 Rh5DG043300 Rh5DG043400 Rh5DG258500
rosa_wichuraiana	Rw0G008330 Rw0G008340 Rw2G045640 Rw5G004480 Rw5G004490 Rw5G022930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	337, 382
AciI	CCGC	4 cut(s)	93, 191, 194, 337
AcoI	YGGCCR	1 cut(s)	78
AcyI	GRCGYC	1 cut(s)	5
AgsI	TTSAA	1 cut(s)	277
AluBI	AGCT	2 cut(s)	115, 133
AluI	AGCT	2 cut(s)	115, 133
Alw26I	GTCTC	2 cut(s)	179, 347
AoxI	GGCC	1 cut(s)	78
ApeKI	GCWGC	1 cut(s)	188
AspA2I	CCTAGG	1 cut(s)	88
AspLEI	GCGC	1 cut(s)	384
AsuHPI	GGTGA	3 cut(s)	9, 107, 263
AvrII	CCTAGG	1 cut(s)	88
BbvI	GCAGC	1 cut(s)	175
BccI	CCATC	3 cut(s)	133, 277, 319
BceAI	ACGGC	1 cut(s)	139
BcgI	CGANNNNNNTGC	2 cut(s)	109, 143
BcoDI	GTCTC	2 cut(s)	179, 347
BfaI	CTAG	1 cut(s)	89
BisI	GCNGC	2 cut(s)	189, 192
BlnI	CCTAGG	1 cut(s)	88
BlpI	GCTNAGC	1 cut(s)	311
BlsI	GCNGC	2 cut(s)	190, 193
Bpu1102I	GCTNAGC	1 cut(s)	311
BsaHI	GRCGYC	1 cut(s)	5
BsaJI	CCNNGG	2 cut(s)	75, 88
BsaWI	WCCGGW	1 cut(s)	231
Bse118I	RCCGGY	1 cut(s)	384
BseDI	CCNNGG	2 cut(s)	75, 88
BseRI	GAGGAG	2 cut(s)	377, 380
BseXI	GCAGC	1 cut(s)	175
Bsh1236I	CGCG	2 cut(s)	337, 382
BshFI	GGCC	1 cut(s)	80
BsiSI	CCGG	3 cut(s)	81, 232, 385
BsmAI	GTCTC	2 cut(s)	179, 347
BsnI	GGCC	1 cut(s)	80
Bsp143I	GATC	1 cut(s)	105
Bsp1720I	GCTNAGC	1 cut(s)	311
Bsp19I	CCATGG	1 cut(s)	75
BspACI	CCGC	4 cut(s)	93, 191, 194, 337
BspANI	GGCC	1 cut(s)	80
BspFNI	CGCG	2 cut(s)	337, 382
BsrFI	RCCGGY	1 cut(s)	384
BssAI	RCCGGY	1 cut(s)	384
BssECI	CCNNGG	2 cut(s)	75, 88
BssMI	GATC	1 cut(s)	105
BssNI	GRCGYC	1 cut(s)	5
BssT1I	CCWWGG	2 cut(s)	75, 88
Bst4CI	ACNGT	1 cut(s)	269
BstACI	GRCGYC	1 cut(s)	5
BstC8I	GCNNGC	1 cut(s)	386
BstDEI	CTNAG	1 cut(s)	311
BstDSI	CCRYGG	1 cut(s)	75
BstFNI	CGCG	2 cut(s)	337, 382
BstHHI	GCGC	1 cut(s)	384
BstKTI	GATC	1 cut(s)	108
BstMAI	GTCTC	2 cut(s)	179, 347
BstMBI	GATC	1 cut(s)	105
BstMWI	GCNNNNNNNGC	1 cut(s)	381
BstUI	CGCG	2 cut(s)	337, 382
BstV1I	GCAGC	1 cut(s)	175
BsuRI	GGCC	1 cut(s)	80
BtgI	CCRYGG	1 cut(s)	75
Cac8I	GCNNGC	1 cut(s)	386
CfoI	GCGC	1 cut(s)	384
Cfr10I	RCCGGY	1 cut(s)	384
CseI	GACGC	1 cut(s)	321
CviAII	CATG	1 cut(s)	76
CviJI	RGCY	7 cut(s)	80, 115, 126, 133, 310, 315, 375
CviKI_1	RGCY	7 cut(s)	80, 115, 126, 133, 310, 315, 375
DdeI	CTNAG	1 cut(s)	311
DpnI	GATC	1 cut(s)	107
DpnII	GATC	1 cut(s)	105
EaeI	YGGCCR	1 cut(s)	78
Eco130I	CCWWGG	2 cut(s)	75, 88
EcoT14I	CCWWGG	2 cut(s)	75, 88
ErhI	CCWWGG	2 cut(s)	75, 88
FaeI	CATG	1 cut(s)	79
FaiI	YATR	3 cut(s)	77, 118, 239
FatI	CATG	1 cut(s)	75
Fnu4HI	GCNGC	2 cut(s)	189, 192
Fsp4HI	GCNGC	2 cut(s)	189, 192
FspBI	CTAG	1 cut(s)	89
GlaI	GCGC	1 cut(s)	383
GluI	GCNGC	2 cut(s)	189, 192
HaeIII	GGCC	1 cut(s)	80
HapII	CCGG	3 cut(s)	81, 232, 385
HgaI	GACGC	1 cut(s)	321
HhaI	GCGC	1 cut(s)	384
Hin1I	GRCGYC	1 cut(s)	5
Hin1II	CATG	1 cut(s)	79
Hin6I	GCGC	1 cut(s)	382
HinP1I	GCGC	1 cut(s)	382
HinfI	GANTC	2 cut(s)	172, 277
HpaII	CCGG	3 cut(s)	81, 232, 385
HphI	GGTGA	3 cut(s)	9, 107, 263
Hpy188I	TCNGA	4 cut(s)	105, 110, 179, 282
Hpy188III	TCNNGA	1 cut(s)	169
Hpy99I	CGWCG	3 cut(s)	183, 269, 337
HpyAV	CCTTC	2 cut(s)	92, 242
HpyCH4III	ACNGT	1 cut(s)	269
HpyCH4IV	ACGT	2 cut(s)	164, 181
HpyCH4V	TGCA	1 cut(s)	120
HpyF10VI	GCNNNNNNNGC	1 cut(s)	381
HpyF3I	CTNAG	1 cut(s)	311
HpySE526I	ACGT	2 cut(s)	164, 181
Hsp92I	GRCGYC	1 cut(s)	5
Hsp92II	CATG	1 cut(s)	79
HspAI	GCGC	1 cut(s)	382
KroI	GCCGGC	1 cut(s)	384
KroNI	GCCGGC	1 cut(s)	386
Kzo9I	GATC	1 cut(s)	105
LpnPI	CCDG	4 cut(s)	94, 185, 245, 330
Lsp1109I	GCAGC	1 cut(s)	175
MaeI	CTAG	1 cut(s)	89
MaeII	ACGT	2 cut(s)	164, 181
MaeIII	GTNAC	4 cut(s)	6, 15, 182, 346
MalI	GATC	1 cut(s)	107
MboI	GATC	1 cut(s)	105
MboII	GAAGA	2 cut(s)	59, 236
MluCI	AATT	1 cut(s)	52
MlyI	GAGTC	1 cut(s)	181
MmeI	TCCRAC	1 cut(s)	202
MnlI	CCTC	3 cut(s)	346, 355, 358
MroNI	GCCGGC	1 cut(s)	384
MseI	TTAA	1 cut(s)	243
MspI	CCGG	3 cut(s)	81, 232, 385
MvnI	CGCG	2 cut(s)	337, 382
MwoI	GCNNNNNNNGC	1 cut(s)	381
NaeI	GCCGGC	1 cut(s)	386
NcoI	CCATGG	1 cut(s)	75
NdeII	GATC	1 cut(s)	105
NgoMIV	GCCGGC	1 cut(s)	384
NlaIII	CATG	1 cut(s)	79
NmuCI	GTSAC	3 cut(s)	6, 15, 346
PdiI	GCCGGC	1 cut(s)	386
PfeI	GAWTC	1 cut(s)	277
PkrI	GCNGC	2 cut(s)	190, 193
PleI	GAGTC	1 cut(s)	180
PpsI	GAGTC	1 cut(s)	180
SaqAI	TTAA	1 cut(s)	243
SatI	GCNGC	2 cut(s)	189, 192
Sau3AI	GATC	1 cut(s)	105
SchI	GAGTC	1 cut(s)	181
SetI	ASST	9 cut(s)	90, 103, 117, 135, 157, 167, 184, 253, 357
Sse9I	AATT	1 cut(s)	52
SsiI	CCGC	4 cut(s)	93, 191, 194, 337
SspMI	CTAG	1 cut(s)	89
StyI	CCWWGG	2 cut(s)	75, 88
TaaI	ACNGT	1 cut(s)	269
TaiI	ACGT	2 cut(s)	167, 184
TaqI	TCGA	2 cut(s)	129, 168
TasI	AATT	1 cut(s)	52
TauI	GCSGC	1 cut(s)	194
TfiI	GAWTC	1 cut(s)	277
Tru1I	TTAA	1 cut(s)	243
Tru9I	TTAA	1 cut(s)	243
TseFI	GTSAC	3 cut(s)	6, 15, 346
TseI	GCWGC	1 cut(s)	188
Tsp45I	GTSAC	3 cut(s)	6, 15, 346
TspDTI	ATGAA	1 cut(s)	237
TspGWI	ACGGA	1 cut(s)	365
XmaJI	CCTAGG	1 cut(s)	88
XspI	CTAG	1 cut(s)	89

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.