RLG00000031316

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000967
Physical Location & Seq
Forward (+)
4052522 .. 4052869
348 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000031316

Sequence Viewer

Length: 348 bp
ATGGCGTGCCAAAACGGAGTGGTGTTTGAAGACTTCTTCCCGGCCATGGTGGACAAGTTGGGAGCGGAAGGCTTCATGAAGGAGCTCTGCAACGGCTTTCGGCTTTTGATGGACGGAGAGAGAGGCGTGATCACGTTTCAGAGCTTGAAGAGGAACTCCGGCTTGTTGGGATTGCAAGGTATGAGCGACGATGAGGTGAGGAGCATGATCAAAGAAGGTGATTTGACTGGTGATGGGTGCTTGAATGAGATGGAGTTTTGTACACTCATGTTTAGGTTGAGTCCTGCTATGATGGAAAGTTCTGTGGAGTTGTTGGAGGAAGCTTTTGTTACTGATGATGGTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

116

Amino Acids

12.67

Weight (kDa)

4.17

Isoelectric Point (pI)

42.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 40 - 92 4.5e-16 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 65
AciI CCGC 1 cut(s) 65
AcoI YGGCCR 1 cut(s) 42
AfaI GTAC 1 cut(s) 262
AfiI CCNNNNNNNGG 1 cut(s) 46
AgsI TTSAA 3 cut(s) 29, 148, 244
AluBI AGCT 3 cut(s) 85, 144, 323
AluI AGCT 3 cut(s) 85, 144, 323
Alw21I GWGCWC 1 cut(s) 87
AoxI GGCC 1 cut(s) 42
AsuC2I CCSGG 1 cut(s) 41
AsuHPI GGTGA 3 cut(s) 208, 230, 242
BanII GRGCYC 1 cut(s) 87
BbsI GAAGAC 1 cut(s) 36
Bbv12I GWGCWC 1 cut(s) 87
BccI CCATC 5 cut(s) 103, 227, 244, 286, 332
BceAI ACGGC 1 cut(s) 109
BclI TGATCA 2 cut(s) 129, 207
BcnI CCSGG 1 cut(s) 41
Bme1390I CCNGG 1 cut(s) 41
BmrFI CCNGG 1 cut(s) 41
BpiI GAAGAC 1 cut(s) 36
BpuMI CCSGG 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 45
Bsc4I CCNNNNNNNGG 1 cut(s) 46
Bse1I ACTGG 1 cut(s) 232
BseDI CCNNGG 1 cut(s) 45
BseLI CCNNNNNNNGG 1 cut(s) 46
BseNI ACTGG 1 cut(s) 232
BseRI GAGGAG 1 cut(s) 214
BshFI GGCC 1 cut(s) 44
BsiHKAI GWGCWC 1 cut(s) 87
BsiSI CCGG 2 cut(s) 41, 159
BslI CCNNNNNNNGG 1 cut(s) 46
BsnI GGCC 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 87
Bsp1407I TGTACA 1 cut(s) 260
Bsp143I GATC 2 cut(s) 129, 207
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 1 cut(s) 65
BspANI GGCC 1 cut(s) 44
BspHI TCATGA 1 cut(s) 75
BsrBI CCGCTC 1 cut(s) 65
BsrGI TGTACA 1 cut(s) 260
BsrI ACTGG 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 2 cut(s) 129, 207
BssT1I CCWWGG 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 143
BstAUI TGTACA 1 cut(s) 260
BstC8I GCNNGC 1 cut(s) 7
BstDSI CCRYGG 1 cut(s) 45
BstKTI GATC 2 cut(s) 132, 210
BstMBI GATC 2 cut(s) 129, 207
BstSCI CCNGG 1 cut(s) 39
BstV2I GAAGAC 1 cut(s) 36
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 45
Cac8I GCNNGC 1 cut(s) 7
CciI TCATGA 1 cut(s) 75
Csp6I GTAC 1 cut(s) 261
CviAII CATG 5 cut(s) 46, 76, 205, 268, 345
CviJI RGCY 8 cut(s) 44, 72, 85, 96, 103, 144, 162, 323
CviKI_1 RGCY 8 cut(s) 44, 72, 85, 96, 103, 144, 162, 323
CviQI GTAC 1 cut(s) 261
DpnI GATC 2 cut(s) 131, 209
DpnII GATC 2 cut(s) 129, 207
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 87
Eco53kI GAGCTC 1 cut(s) 85
EcoICRI GAGCTC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 5 cut(s) 49, 79, 208, 271, 348
FaiI YATR 7 cut(s) 47, 77, 182, 206, 269, 290, 346
FatI CATG 5 cut(s) 45, 75, 204, 267, 344
FbaI TGATCA 2 cut(s) 129, 207
FriOI GRGCYC 1 cut(s) 87
HaeIII GGCC 1 cut(s) 44
HapII CCGG 2 cut(s) 41, 159
Hin1II CATG 5 cut(s) 49, 79, 208, 271, 348
HindIII AAGCTT 1 cut(s) 321
HinfI GANTC 1 cut(s) 280
HpaII CCGG 2 cut(s) 41, 159
HphI GGTGA 3 cut(s) 208, 230, 242
Hpy166II GTNNAC 2 cut(s) 52, 263
Hpy188I TCNGA 1 cut(s) 141
Hpy188III TCNNGA 1 cut(s) 76
Hpy8I GTNNAC 2 cut(s) 52, 263
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 3 cut(s) 62, 73, 209
HpyCH4IV ACGT 1 cut(s) 134
HpyCH4V TGCA 3 cut(s) 90, 175, 344
HpySE526I ACGT 1 cut(s) 134
Hsp92II CATG 5 cut(s) 49, 79, 208, 271, 348
Ksp22I TGATCA 2 cut(s) 129, 207
Kzo9I GATC 2 cut(s) 129, 207
LmnI GCTCC 3 cut(s) 62, 82, 201
LpnPI CCDG 4 cut(s) 54, 172, 213, 297
MaeII ACGT 1 cut(s) 134
MaeIII GTNAC 1 cut(s) 328
MalI GATC 2 cut(s) 131, 209
MbiI CCGCTC 1 cut(s) 65
MboI GATC 2 cut(s) 129, 207
MboII GAAGA 3 cut(s) 28, 41, 160
MhlI GDGCHC 1 cut(s) 87
MlyI GAGTC 1 cut(s) 289
MmeI TCCRAC 1 cut(s) 294
MnlI CCTC 5 cut(s) 116, 144, 187, 192, 310
MspI CCGG 2 cut(s) 41, 159
MspR9I CCNGG 1 cut(s) 41
NciI CCSGG 1 cut(s) 41
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 129, 207
NlaIII CATG 5 cut(s) 49, 79, 208, 271, 348
PagI TCATGA 1 cut(s) 75
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
Psp124BI GAGCTC 1 cut(s) 87
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
SacI GAGCTC 1 cut(s) 87
Sau3AI GATC 2 cut(s) 129, 207
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 1 cut(s) 41
SduI GDGCHC 1 cut(s) 87
SetI ASST 8 cut(s) 87, 137, 146, 181, 198, 220, 278, 325
SsiI CCGC 1 cut(s) 65
SstI GAGCTC 1 cut(s) 87
StyD4I CCNGG 1 cut(s) 39
StyI CCWWGG 1 cut(s) 45
TaiI ACGT 1 cut(s) 137
TatI WGTACW 1 cut(s) 260
TspDTI ATGAA 2 cut(s) 64, 92
TspGWI ACGGA 2 cut(s) 30, 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.