FvH4_6g44060

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000967
Physical Location & Seq
Reverse (-)
33974947 .. 33975468
522 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44060.t1

Sequence Viewer

Length: 375 bp
ATGGCAGGAGTTGGCAACAAGGCAAATAATGTTGATTTCGAAGACTTGTTACCGGAGATGGCGGACAAGCTCGGCGGCGAGGGGCTGATAAGGGAGCTATGCAATGGCTTCCAGCTGCTGATGGACAAGGAGAGAGGTGTCATTACGCTAGAGAGCTTGAGGAGGAACTCTGCTGTTTTGGGCCTTCAGGATTTGAGAGAGGATGAGCTTGTGAGTATGTTGAAGGAAGGTGACTTGGATGGAGATGGGGCTCTGAATCAGATGGAGTTCTGCGTTTTGATGTTCAGATTGAGTCCTGAGTTGATGGAGGAGAGTTGGATTTGGCTTGAGCAGGGCTTGCAGGAGGAGCTAAATAGTAATACCAAGAGACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03420 map03420
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

125

Amino Acids

13.97

Weight (kDa)

4.28

Isoelectric Point (pI)

46.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 44 - 96 7.1e-20 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 62, 75
AcuI CTGAAG 1 cut(s) 170
AgsI TTSAA 1 cut(s) 223
AluBI AGCT 6 cut(s) 70, 97, 115, 156, 208, 349
AluI AGCT 6 cut(s) 70, 97, 115, 156, 208, 349
Alw26I GTCTC 1 cut(s) 361
AlwNI CAGNNNCTG 1 cut(s) 118
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 242
AsuII TTCGAA 1 cut(s) 39
BanII GRGCYC 1 cut(s) 253
BarI GAAGNNNNNNTAC 2 cut(s) 33, 65
BbsI GAAGAC 1 cut(s) 48
BbvI GCAGC 1 cut(s) 102
BccI CCATC 6 cut(s) 52, 115, 233, 239, 256, 298
BcoDI GTCTC 1 cut(s) 361
BfaI CTAG 1 cut(s) 149
BisI GCNGC 2 cut(s) 76, 116
BlsI GCNGC 2 cut(s) 77, 117
BmgT120I GGNCC 1 cut(s) 181
BpiI GAAGAC 1 cut(s) 48
Bpu14I TTCGAA 1 cut(s) 39
BpuEI CTTGAG 2 cut(s) 178, 347
BsaI GGTCTC 1 cut(s) 361
BsaWI WCCGGW 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 109
BseGI GGATG 2 cut(s) 208, 244
BseMI GCAATG 1 cut(s) 109
BseMII CTCAG 1 cut(s) 288
BseRI GAGGAG 3 cut(s) 175, 323, 359
BseXI GCAGC 1 cut(s) 102
BshFI GGCC 1 cut(s) 183
BsiSI CCGG 1 cut(s) 53
BsmAI GTCTC 1 cut(s) 361
BsnI GGCC 1 cut(s) 183
Bso31I GGTCTC 1 cut(s) 361
Bsp119I TTCGAA 1 cut(s) 39
Bsp1286I GDGCHC 1 cut(s) 253
BspACI CCGC 2 cut(s) 62, 75
BspANI GGCC 1 cut(s) 183
BspCNI CTCAG 1 cut(s) 289
BspT104I TTCGAA 1 cut(s) 39
BspTNI GGTCTC 1 cut(s) 361
BsrDI GCAATG 1 cut(s) 109
BstAPI GCANNNNNTGC 1 cut(s) 337
BstBI TTCGAA 1 cut(s) 39
BstC8I GCNNGC 1 cut(s) 338
BstDEI CTNAG 1 cut(s) 297
BstF5I GGATG 2 cut(s) 208, 244
BstMAI GTCTC 1 cut(s) 361
BstMWI GCNNNNNNNGC 2 cut(s) 337, 346
BstV1I GCAGC 1 cut(s) 102
BstV2I GAAGAC 1 cut(s) 48
BsuRI GGCC 1 cut(s) 183
BtsCI GGATG 2 cut(s) 208, 244
Cac8I GCNNGC 1 cut(s) 338
CaiI CAGNNNCTG 1 cut(s) 118
Cfr13I GGNCC 1 cut(s) 181
DdeI CTNAG 1 cut(s) 297
EciI GGCGGA 1 cut(s) 77
Eco24I GRGCYC 1 cut(s) 253
Eco31I GGTCTC 1 cut(s) 361
Eco57I CTGAAG 1 cut(s) 170
EcoT38I GRGCYC 1 cut(s) 253
FaiI YATR 2 cut(s) 100, 218
Fnu4HI GCNGC 2 cut(s) 76, 116
FokI GGATG 2 cut(s) 215, 251
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 2 cut(s) 76, 116
FspBI CTAG 1 cut(s) 149
GluI GCNGC 2 cut(s) 76, 116
HaeIII GGCC 1 cut(s) 183
HapII CCGG 1 cut(s) 53
HinfI GANTC 2 cut(s) 256, 292
HpaII CCGG 1 cut(s) 53
HphI GGTGA 1 cut(s) 242
Hpy188I TCNGA 3 cut(s) 255, 261, 287
Hpy188III TCNNGA 2 cut(s) 188, 296
HpyAV CCTTC 3 cut(s) 194, 217, 221
HpyCH4V TGCA 2 cut(s) 102, 340
HpyF10VI GCNNNNNNNGC 2 cut(s) 337, 346
HpyF3I CTNAG 1 cut(s) 297
LmnI GCTCC 2 cut(s) 94, 346
LpnPI CCDG 6 cut(s) 66, 125, 173, 309, 317, 326
Lsp1109I GCAGC 1 cut(s) 102
MaeI CTAG 1 cut(s) 149
MaeIII GTNAC 2 cut(s) 48, 230
MboII GAAGA 1 cut(s) 53
MhlI GDGCHC 1 cut(s) 253
MlyI GAGTC 1 cut(s) 301
MmeI TCCRAC 1 cut(s) 296
MnlI CCTC 7 cut(s) 73, 128, 153, 156, 193, 301, 337
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 1 cut(s) 53
MwoI GCNNNNNNNGC 2 cut(s) 337, 346
NmeAIII GCCGAG 1 cut(s) 51
NmuCI GTSAC 1 cut(s) 230
NspV TTCGAA 1 cut(s) 39
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 2 cut(s) 77, 117
PleI GAGTC 1 cut(s) 300
PpsI GAGTC 1 cut(s) 300
PspPI GGNCC 1 cut(s) 181
PstNI CAGNNNCTG 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 115
SatI GCNGC 2 cut(s) 76, 116
Sau96I GGNCC 1 cut(s) 181
SchI GAGTC 1 cut(s) 301
SduI GDGCHC 1 cut(s) 253
SetI ASST 9 cut(s) 72, 99, 117, 139, 158, 210, 232, 351, 373
SfuI TTCGAA 1 cut(s) 39
SmlI CTYRAG 2 cut(s) 157, 326
SmoI CTYRAG 2 cut(s) 157, 326
SsiI CCGC 2 cut(s) 62, 75
SspMI CTAG 1 cut(s) 149
TaqI TCGA 1 cut(s) 39
TauI GCSGC 1 cut(s) 78
TfiI GAWTC 1 cut(s) 256
TseFI GTSAC 1 cut(s) 230
TseI GCWGC 1 cut(s) 115
Tsp45I GTSAC 1 cut(s) 230
XspI CTAG 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.