Prupe.4G033500_v2.0.a1

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp04
OG0000967
Physical Location & Seq
Forward (+)
1559883 .. 1560893
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.4G033500.1

Sequence Viewer

Length: 348 bp
ATGGCTTGTCGAAATGGGGTTGTGTTTGAAGATTTCTTTCCTGCCATGGTTGACAAATTGGGAGCAGAAGGTTTCATGAAGGAGCTCTGCAATGGGTTCCGGTTGTTGATGGACAGAGAGAAGGGCGTGATCACATTTGAGAGCTTGAAGAGGAACTCGGGCCTGTTGGGGTTGGAAGGCATGAGTGATGAGGAGATCATGTGCATGCTGAGGGAAGGTGATTTGGATGGTGATGGGAGTTTGAATGAGATGGAGTTTTGTACCCTCATGTTCAGATTGAGCCCTGCCATGATGCAAACTTCTAAGGAATTATTGGTGGAAGCTTTAGATTTAGATAGTGAAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

116

Amino Acids

12.92

Weight (kDa)

4.27

Isoelectric Point (pI)

42.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 262
AgsI TTSAA 3 cut(s) 29, 148, 244
AluBI AGCT 3 cut(s) 85, 144, 323
AluI AGCT 3 cut(s) 85, 144, 323
Alw21I GWGCWC 1 cut(s) 87
Ama87I CYCGRG 1 cut(s) 157
AoxI GGCC 1 cut(s) 160
AspS9I GGNCC 1 cut(s) 160
AsuHPI GGTGA 2 cut(s) 230, 242
AvaI CYCGRG 1 cut(s) 157
BanII GRGCYC 2 cut(s) 87, 284
Bbv12I GWGCWC 1 cut(s) 87
BbvCI CCTCAGC 1 cut(s) 209
BccI CCATC 4 cut(s) 103, 221, 227, 244
BclI TGATCA 1 cut(s) 129
BmeT110I CYCGRG 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 160
BmiI GGNNCC 1 cut(s) 98
BmsI GCATC 1 cut(s) 282
Bpu10I CCTNAGC 1 cut(s) 209
BsaJI CCNNGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 99
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bse3DI GCAATG 1 cut(s) 97
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 232
BseMI GCAATG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 200
BseRI GAGGAG 1 cut(s) 206
BshFI GGCC 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 87
BsiHKCI CYCGRG 1 cut(s) 157
BsiSI CCGG 1 cut(s) 100
BsnI GGCC 1 cut(s) 162
BsoBI CYCGRG 1 cut(s) 157
Bsp1286I GDGCHC 2 cut(s) 87, 284
Bsp143I GATC 2 cut(s) 129, 195
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 1 cut(s) 162
BspCNI CTCAG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 98
BsrDI GCAATG 1 cut(s) 97
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 2 cut(s) 129, 195
BssT1I CCWWGG 1 cut(s) 45
Bst6I CTCTTC 1 cut(s) 143
BstC8I GCNNGC 1 cut(s) 206
BstDEI CTNAG 2 cut(s) 209, 303
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 232
BstKTI GATC 2 cut(s) 132, 198
BstMBI GATC 2 cut(s) 129, 195
BstNSI RCATGY 1 cut(s) 208
BsuRI GGCC 1 cut(s) 162
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 232
Cac8I GCNNGC 1 cut(s) 206
CciI TCATGA 1 cut(s) 75
Cfr13I GGNCC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 261
CviAII CATG 7 cut(s) 46, 76, 181, 199, 205, 268, 289
CviJI RGCY 6 cut(s) 5, 85, 144, 162, 282, 323
CviKI_1 RGCY 6 cut(s) 5, 85, 144, 162, 282, 323
CviQI GTAC 1 cut(s) 261
DdeI CTNAG 2 cut(s) 209, 303
DpnI GATC 2 cut(s) 131, 197
DpnII GATC 2 cut(s) 129, 195
Eam1104I CTCTTC 1 cut(s) 143
EarI CTCTTC 1 cut(s) 143
Ecl136II GAGCTC 1 cut(s) 85
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 2 cut(s) 87, 284
Eco53kI GAGCTC 1 cut(s) 85
Eco88I CYCGRG 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 85
EcoT14I CCWWGG 1 cut(s) 45
EcoT38I GRGCYC 2 cut(s) 87, 284
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 7 cut(s) 49, 79, 184, 202, 208, 271, 292
FaiI YATR 7 cut(s) 47, 77, 182, 200, 206, 269, 290
FatI CATG 7 cut(s) 45, 75, 180, 198, 204, 267, 288
FbaI TGATCA 1 cut(s) 129
FokI GGATG 1 cut(s) 239
FriOI GRGCYC 2 cut(s) 87, 284
HaeIII GGCC 1 cut(s) 162
HapII CCGG 1 cut(s) 100
Hin1II CATG 7 cut(s) 49, 79, 184, 202, 208, 271, 292
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 321
HpaII CCGG 1 cut(s) 100
HphI GGTGA 2 cut(s) 230, 242
Hpy166II GTNNAC 1 cut(s) 52
Hpy188I TCNGA 1 cut(s) 275
Hpy188III TCNNGA 1 cut(s) 76
Hpy8I GTNNAC 1 cut(s) 52
HpyAV CCTTC 5 cut(s) 62, 73, 115, 170, 209
HpyCH4V TGCA 3 cut(s) 90, 204, 295
HpyF3I CTNAG 2 cut(s) 209, 303
Hsp92II CATG 7 cut(s) 49, 79, 184, 202, 208, 271, 292
Ksp22I TGATCA 1 cut(s) 129
Kzo9I GATC 2 cut(s) 129, 195
LmnI GCTCC 2 cut(s) 62, 82
LpnPI CCDG 4 cut(s) 54, 113, 176, 297
LweI GCATC 1 cut(s) 282
MalI GATC 2 cut(s) 131, 197
MboI GATC 2 cut(s) 129, 195
MboII GAAGA 2 cut(s) 41, 160
MhlI GDGCHC 2 cut(s) 87, 284
MluCI AATT 2 cut(s) 56, 308
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 144, 184, 204, 275
MslI CAYNNNNRTG 1 cut(s) 203
MspI CCGG 1 cut(s) 100
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 129, 195
NlaIII CATG 7 cut(s) 49, 79, 184, 202, 208, 271, 292
NlaIV GGNNCC 1 cut(s) 98
NspI RCATGY 1 cut(s) 208
PaeI GCATGC 1 cut(s) 208
PagI TCATGA 1 cut(s) 75
Psp124BI GAGCTC 1 cut(s) 87
PspN4I GGNNCC 1 cut(s) 98
PspPI GGNCC 1 cut(s) 160
RsaI GTAC 1 cut(s) 262
RsaNI GTAC 1 cut(s) 261
RseI CAYNNNNRTG 1 cut(s) 203
SacI GAGCTC 1 cut(s) 87
Sau3AI GATC 2 cut(s) 129, 195
Sau96I GGNCC 1 cut(s) 160
SduI GDGCHC 2 cut(s) 87, 284
SetI ASST 5 cut(s) 73, 87, 146, 220, 325
SfaNI GCATC 1 cut(s) 282
SmiMI CAYNNNNRTG 1 cut(s) 203
SphI GCATGC 1 cut(s) 208
Sse9I AATT 2 cut(s) 56, 308
SstI GAGCTC 1 cut(s) 87
StyI CCWWGG 1 cut(s) 45
TaqI TCGA 1 cut(s) 10
TasI AATT 2 cut(s) 56, 308
TspDTI ATGAA 2 cut(s) 64, 92
XceI RCATGY 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.