RchiOBHm_Chr5g0005661

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000967
Physical Location & Seq
Forward (+)
3611157 .. 3611671
515 bp
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UTR
Exon/CDS
Intron
PRQ28686

Sequence Viewer

Length: 348 bp
ATGGCGTCCAGAAGTGGATTGGTTGTGTTCGAGGATTCGTTTCCGACGATGTTGGAGAGGTTAGGCGAGGAAGGGTTTATGAGGGAGTTGTGCAACGGGTTCCGTCTGCTGAGGGACGAGAAGAGAGAGGTGATCACATTCGAGAGCTTGAAGAAGAACTCGGCGCTGTTGGGGTTGCAGGGAATGAGTGATGAAGAGTTGAGGTGCATGGTGAGAGAAGGTGACTTAGATGGAGATGGGTGTCTGAGTGAGATGGAGTTTTGTACTCTCATGTTTAGGTTAAGTCCTGCTTTGATGCAGACTTCTAAGGAATTGTTGGAGGAGGCTATAGTAAGCAGTAGGTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

115

Amino Acids

13.04

Weight (kDa)

4.6

Isoelectric Point (pI)

58.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 43 - 93 2.2e-19 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 265
AgsI TTSAA 1 cut(s) 151
AluBI AGCT 1 cut(s) 147
AluI AGCT 1 cut(s) 147
AspLEI GCGC 1 cut(s) 166
AsuHPI GGTGA 3 cut(s) 142, 223, 233
BbvCI CCTCAGC 1 cut(s) 110
BccI CCATC 3 cut(s) 224, 230, 247
BclI TGATCA 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 327
BfoI RGCGCY 1 cut(s) 167
BmiI GGNNCC 1 cut(s) 101
BmsI GCATC 1 cut(s) 285
Bpu10I CCTNAGC 1 cut(s) 110
BsaHI GRCGYC 1 cut(s) 5
BseMII CTCAG 2 cut(s) 101, 236
BseRI GAGGAG 1 cut(s) 335
BslFI GGGAC 1 cut(s) 128
BsmFI GGGAC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 132
BspCNI CTCAG 2 cut(s) 102, 237
BspLI GGNNCC 1 cut(s) 101
BssMI GATC 1 cut(s) 132
BssNI GRCGYC 1 cut(s) 5
Bst6I CTCTTC 2 cut(s) 116, 189
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 342
BstDEI CTNAG 4 cut(s) 110, 226, 245, 306
BstH2I RGCGCY 1 cut(s) 167
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 1 cut(s) 135
BstMBI GATC 1 cut(s) 132
BstMWI GCNNNNNNNGC 1 cut(s) 342
BstSFI CTRYAG 1 cut(s) 327
CfoI GCGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 264
CviAII CATG 2 cut(s) 208, 271
CviJI RGCY 2 cut(s) 147, 326
CviKI_1 RGCY 2 cut(s) 147, 326
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 4 cut(s) 110, 226, 245, 306
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
Eam1104I CTCTTC 2 cut(s) 116, 189
EarI CTCTTC 2 cut(s) 116, 189
FaeI CATG 2 cut(s) 211, 274
FaiI YATR 4 cut(s) 80, 209, 272, 329
FalI AAGNNNNNCTT 2 cut(s) 274, 306
FaqI GGGAC 1 cut(s) 128
FatI CATG 2 cut(s) 207, 270
FbaI TGATCA 1 cut(s) 132
GlaI GCGC 1 cut(s) 165
HaeII RGCGCY 1 cut(s) 167
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 211, 274
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 1 cut(s) 35
HphI GGTGA 3 cut(s) 142, 223, 233
Hpy188I TCNGA 2 cut(s) 45, 246
Hpy188III TCNNGA 2 cut(s) 9, 142
Hpy99I CGWCG 1 cut(s) 49
HpyAV CCTTC 2 cut(s) 65, 212
HpyCH4V TGCA 4 cut(s) 93, 178, 207, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 342
HpyF3I CTNAG 4 cut(s) 110, 226, 245, 306
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 211, 274
HspAI GCGC 1 cut(s) 164
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 1 cut(s) 132
LpnPI CCDG 3 cut(s) 22, 164, 300
LweI GCATC 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 221
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 4 cut(s) 133, 163, 166, 206
MluCI AATT 1 cut(s) 311
MmeI TCCRAC 3 cut(s) 33, 68, 297
MnlI CCTC 9 cut(s) 25, 51, 61, 75, 105, 121, 195, 313, 316
MseI TTAA 1 cut(s) 281
MwoI GCNNNNNNNGC 1 cut(s) 342
NdeII GATC 1 cut(s) 132
NlaIII CATG 2 cut(s) 211, 274
NlaIV GGNNCC 1 cut(s) 101
NmeAIII GCCGAG 1 cut(s) 140
NmuCI GTSAC 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 44
PfeI GAWTC 1 cut(s) 35
PspN4I GGNNCC 1 cut(s) 101
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SaqAI TTAA 1 cut(s) 281
Sau3AI GATC 1 cut(s) 132
SetI ASST 7 cut(s) 62, 132, 149, 206, 223, 281, 344
SfaNI GCATC 1 cut(s) 285
SfcI CTRYAG 1 cut(s) 327
Sse9I AATT 1 cut(s) 311
TaqI TCGA 2 cut(s) 30, 141
TasI AATT 1 cut(s) 311
TatI WGTACW 1 cut(s) 263
TfiI GAWTC 1 cut(s) 35
Tru1I TTAA 1 cut(s) 281
Tru9I TTAA 1 cut(s) 281
TseFI GTSAC 1 cut(s) 221
Tsp45I GTSAC 1 cut(s) 221
TspDTI ATGAA 1 cut(s) 207
TspGWI ACGGA 1 cut(s) 92
XcmI CCANNNNNNNNNTGG 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.