Rw5G022930

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000967
Physical Location & Seq
Forward (+)
32110247 .. 32110606
360 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022930.1

Sequence Viewer

Length: 360 bp
ATGGCTTCTCCTCAGCAAAACGCACAAGCAAATTTCCAAGACTTGCTGCCCACCATGGCCGACAAGCTCGGCGGGGACGGTTTGATCGGCGAGCTCTGTAATGGGTTCAATCTACTGATGGATCCCGCCAAAGGGGTCATCACCTTCGATAGCCTCAAGAGGAACTCAGCCCTGCTGGGCTTGCAGGACCTGGGCGACGACGATCTACGGTCTATGTTGCAGGAAGGCGATTTCGACGGCGATGGGGCGCTAAATCAGATGGAGTTTTGCGTGTTGATGTTTAGATTGAGTCCCGAGCTTATGGAGGAGTCTCGTCTCTGGCTGGACGATGCTCTTCAATACGAGTTTAAGCATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03420 map03420
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

119

Amino Acids

13.23

Weight (kDa)

4.07

Isoelectric Point (pI)

52.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 44 - 95 9.4e-21 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 72, 126
AclWI GGATC 2 cut(s) 116, 129
AcoI YGGCCR 1 cut(s) 57
AcsI RAATTY 1 cut(s) 31
AfiI CCNNNNNNNGG 2 cut(s) 131, 132
AgsI TTSAA 2 cut(s) 109, 338
AjnI CCWGG 1 cut(s) 189
AluBI AGCT 3 cut(s) 67, 94, 298
AluI AGCT 3 cut(s) 67, 94, 298
Alw21I GWGCWC 1 cut(s) 96
Alw26I GTCTC 2 cut(s) 315, 320
AlwI GGATC 2 cut(s) 116, 129
AlwNI CAGNNNCTG 1 cut(s) 190
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 1 cut(s) 57
ApeKI GCWGC 1 cut(s) 46
ApoI RAATTY 1 cut(s) 31
AspLEI GCGC 1 cut(s) 250
AspS9I GGNCC 1 cut(s) 187
AsuHPI GGTGA 1 cut(s) 133
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 187
BamHI GGATCC 1 cut(s) 121
BanII GRGCYC 1 cut(s) 96
Bbv12I GWGCWC 1 cut(s) 96
BbvCI CCTCAGC 1 cut(s) 12
BbvI GCAGC 1 cut(s) 33
BccI CCATC 3 cut(s) 112, 236, 253
BceAI ACGGC 1 cut(s) 253
BciT130I CCWGG 1 cut(s) 191
BcoDI GTCTC 2 cut(s) 315, 320
BfoI RGCGCY 1 cut(s) 251
BisI GCNGC 1 cut(s) 47
BlsI GCNGC 1 cut(s) 48
Bme1390I CCNGG 1 cut(s) 191
Bme18I GGWCC 1 cut(s) 187
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 1 cut(s) 187
BmiI GGNNCC 1 cut(s) 123
BmrFI CCNGG 1 cut(s) 191
BmsI GCATC 1 cut(s) 319
Bpu10I CCTNAGC 1 cut(s) 12
BpuEI CTTGAG 1 cut(s) 140
BsaJI CCNNGG 2 cut(s) 54, 190
Bsc4I CCNNNNNNNGG 2 cut(s) 131, 132
Bse3DI GCAATG 1 cut(s) 352
BseBI CCWGG 1 cut(s) 191
BseDI CCNNGG 2 cut(s) 54, 190
BseLI CCNNNNNNNGG 2 cut(s) 131, 132
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 2 cut(s) 26, 180
BseRI GAGGAG 1 cut(s) 320
BseXI GCAGC 1 cut(s) 33
BseYI CCCAGC 1 cut(s) 175
BshFI GGCC 1 cut(s) 59
BsiHKAI GWGCWC 1 cut(s) 96
BsiHKCI CYCGRG 1 cut(s) 293
BslFI GGGAC 2 cut(s) 89, 276
BslI CCNNNNNNNGG 2 cut(s) 131, 132
BsmAI GTCTC 2 cut(s) 315, 320
BsmBI CGTCTC 1 cut(s) 320
BsmFI GGGAC 2 cut(s) 89, 276
BsnI GGCC 1 cut(s) 59
BsoBI CYCGRG 1 cut(s) 293
Bsp1286I GDGCHC 1 cut(s) 96
Bsp143I GATC 3 cut(s) 84, 121, 202
Bsp19I CCATGG 1 cut(s) 54
BspACI CCGC 2 cut(s) 72, 126
BspANI GGCC 1 cut(s) 59
BspCNI CTCAG 2 cut(s) 25, 179
BspLI GGNNCC 1 cut(s) 123
BspPI GGATC 2 cut(s) 116, 129
BspQI GCTCTTC 1 cut(s) 339
BsrDI GCAATG 1 cut(s) 352
BssECI CCNNGG 2 cut(s) 54, 190
BssMI GATC 3 cut(s) 84, 121, 202
BssT1I CCWWGG 1 cut(s) 54
Bst2UI CCWGG 1 cut(s) 191
Bst4CI ACNGT 2 cut(s) 80, 210
Bst6I CTCTTC 1 cut(s) 339
BstC8I GCNNGC 2 cut(s) 92, 182
BstDEI CTNAG 2 cut(s) 12, 166
BstDSI CCRYGG 1 cut(s) 54
BstH2I RGCGCY 1 cut(s) 251
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 3 cut(s) 87, 124, 205
BstMAI GTCTC 2 cut(s) 315, 320
BstMBI GATC 3 cut(s) 84, 121, 202
BstMWI GCNNNNNNNGC 1 cut(s) 181
BstNI CCWGG 1 cut(s) 191
BstSCI CCNGG 1 cut(s) 189
BstV1I GCAGC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
BsuRI GGCC 1 cut(s) 59
BtgI CCRYGG 1 cut(s) 54
BtgZI GCGATG 1 cut(s) 255
Cac8I GCNNGC 2 cut(s) 92, 182
CaiI CAGNNNCTG 1 cut(s) 190
CfoI GCGC 1 cut(s) 250
Cfr13I GGNCC 1 cut(s) 187
CviAII CATG 1 cut(s) 55
CviJI RGCY 9 cut(s) 5, 59, 67, 94, 153, 170, 180, 298, 322
CviKI_1 RGCY 9 cut(s) 5, 59, 67, 94, 153, 170, 180, 298, 322
DdeI CTNAG 2 cut(s) 12, 166
DpnI GATC 3 cut(s) 86, 123, 204
DpnII GATC 3 cut(s) 84, 121, 202
EaeI YGGCCR 1 cut(s) 57
Eam1104I CTCTTC 1 cut(s) 339
EarI CTCTTC 1 cut(s) 339
Ecl136II GAGCTC 1 cut(s) 94
Eco130I CCWWGG 1 cut(s) 54
Eco24I GRGCYC 1 cut(s) 96
Eco47I GGWCC 1 cut(s) 187
Eco53kI GAGCTC 1 cut(s) 94
Eco88I CYCGRG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 94
EcoO109I RGGNCCY 1 cut(s) 187
EcoRII CCWGG 1 cut(s) 189
EcoT14I CCWWGG 1 cut(s) 54
EcoT38I GRGCYC 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 54
Esp3I CGTCTC 1 cut(s) 320
FaeI CATG 1 cut(s) 58
FaiI YATR 3 cut(s) 56, 215, 302
FaqI GGGAC 2 cut(s) 89, 276
FatI CATG 1 cut(s) 54
FauI CCCGC 2 cut(s) 65, 133
Fnu4HI GCNGC 1 cut(s) 47
FriOI GRGCYC 1 cut(s) 96
Fsp4HI GCNGC 1 cut(s) 47
GlaI GCGC 1 cut(s) 249
GluI GCNGC 1 cut(s) 47
GsaI CCCAGC 1 cut(s) 179
HaeII RGCGCY 1 cut(s) 251
HaeIII GGCC 1 cut(s) 59
HhaI GCGC 1 cut(s) 250
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HinfI GANTC 2 cut(s) 289, 308
HphI GGTGA 1 cut(s) 133
Hpy188I TCNGA 1 cut(s) 258
Hpy188III TCNNGA 2 cut(s) 157, 293
Hpy99I CGWCG 3 cut(s) 200, 203, 239
HpyAV CCTTC 2 cut(s) 154, 218
HpyCH4III ACNGT 2 cut(s) 80, 210
HpyCH4V TGCA 2 cut(s) 184, 220
HpyF10VI GCNNNNNNNGC 1 cut(s) 181
HpyF3I CTNAG 2 cut(s) 12, 166
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 248
Kzo9I GATC 3 cut(s) 84, 121, 202
LguI GCTCTTC 1 cut(s) 339
LpnPI CCDG 8 cut(s) 161, 170, 176, 185, 203, 206, 304, 308
Lsp1109I GCAGC 1 cut(s) 33
LweI GCATC 1 cut(s) 319
MalI GATC 3 cut(s) 86, 123, 204
MboI GATC 3 cut(s) 84, 121, 202
MboII GAAGA 1 cut(s) 326
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 1 cut(s) 96
MluCI AATT 1 cut(s) 31
MlyI GAGTC 2 cut(s) 298, 317
MnlI CCTC 4 cut(s) 21, 153, 164, 298
MseI TTAA 1 cut(s) 348
MspR9I CCNGG 1 cut(s) 191
MvaI CCWGG 1 cut(s) 191
MwoI GCNNNNNNNGC 1 cut(s) 181
NcoI CCATGG 1 cut(s) 54
NdeII GATC 3 cut(s) 84, 121, 202
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 123
NmeAIII GCCGAG 1 cut(s) 48
PciSI GCTCTTC 1 cut(s) 339
PkrI GCNGC 1 cut(s) 48
PleI GAGTC 2 cut(s) 297, 316
PpsI GAGTC 2 cut(s) 297, 316
PpuMI RGGWCCY 1 cut(s) 187
Psp124BI GAGCTC 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 187
Psp6I CCWGG 1 cut(s) 189
PspFI CCCAGC 1 cut(s) 175
PspGI CCWGG 1 cut(s) 189
PspN4I GGNNCC 1 cut(s) 123
PspPI GGNCC 1 cut(s) 187
PspPPI RGGWCCY 1 cut(s) 187
PstNI CAGNNNCTG 1 cut(s) 190
PsuI RGATCY 1 cut(s) 121
SacI GAGCTC 1 cut(s) 96
SapI GCTCTTC 1 cut(s) 339
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 1 cut(s) 47
Sau3AI GATC 3 cut(s) 84, 121, 202
Sau96I GGNCC 1 cut(s) 187
SchI GAGTC 2 cut(s) 298, 317
ScrFI CCNGG 1 cut(s) 191
SduI GDGCHC 1 cut(s) 96
SetI ASST 5 cut(s) 69, 96, 146, 192, 300
SfaNI GCATC 1 cut(s) 319
SinI GGWCC 1 cut(s) 187
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 1 cut(s) 31
SsiI CCGC 2 cut(s) 72, 126
SstI GAGCTC 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 189
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 2 cut(s) 80, 210
TaqI TCGA 2 cut(s) 147, 234
TasI AATT 1 cut(s) 31
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TseI GCWGC 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 187
XapI RAATTY 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.