Rorug04G0417100

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000967
Physical Location & Seq
Reverse (-)
57840912 .. 57843102
2191 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0417100.1

Sequence Viewer

Length: 1038 bp
ATGAGTTTCTTAACAGGATCATCGAGCCATTCGTGGCATCCAATCATGGCTACGGCTACTACTGCTCCAAGGTATTGGTGGAACTGGAGGTTCTTGACCGGTGCAATATTTATATTGTCTAGTATAGTTATTGCTGCAGTTCTTATAAGGAAATATGAAGGCTGCAAAAAATCCAGGAGGGAAAGAAGAGAAAATCAGCGTGAAACAGTAGGATCTTTATATGAGGATGAAGCCTGGAGGACATCTTTCAAAGTAATTCATCCCGCTTGGCTTCTTGCGTACAGAATAGCTGCTTTCGTTGTGCTTCTGGCCTTGATAATTACCAATTTCATTCTTGATGGAGCTGTCATAGTTTTCTTCTATACTCAGTGGACATTTTGTTTGGTCACAATCTATTTTGCGATTGGAATTTCACTCTCCATTTATGGATGCTACAAATACCGCAATAGAGTTGGCGAGGATGCAGCTGATTATGTGAGAGATGAAGAGGAAGGTACATATATTGCTCCAACACTGGGCGAAAATGAAGATACATTCAACACGTCTAAAAGTTTTACTGATGAAGAATGTCCCTCCCGCAAAGCCGCAGGAGTATATGTTTATATCTTTCAAGTCATTTATCAGATGTGTGGGGGTGCAGTGGTGCTCACTGATGTTGTATTTTGGTCCCTTCTGTATCCAAATCTAGATAGCGAATATGACGAACTGGACTTTATGATGGCCAGTATGCATTCATTCAATCTTGGTCTCCTCCTTGGTGATACAATTTTGAATGGCATGCGGTTTCCAATGTTTCGAATCTCATATTTTGTGCTATGGACGGGATTATTCGTGATTTTCCAGTGGATAATCCACGGTTGTATAGACATGTGGTGGCCTTATCCATTTCTCGACTTGTCATCCAATTATGCACCCTTATGGTATCTTGGGGTCGGAGTGGCGCATATCCCATGCTACGGCGCCTATGCTTTAATTATTAGGATGAAGCAGTTTTGGTTCTCAAGATCATTCCCGAAGTCTTTTCAGGGTGAGAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

345

Amino Acids

40.02

Weight (kDa)

6.09

Isoelectric Point (pI)

32.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 146
AccB1I GGYRCC 1 cut(s) 959
AciI CCGC 5 cut(s) 264, 442, 577, 585, 781
AclWI GGATC 2 cut(s) 25, 220
AcoI YGGCCR 1 cut(s) 720
AcsI RAATTY 1 cut(s) 408
AcyI GRCGYC 1 cut(s) 960
AfaI GTAC 2 cut(s) 281, 496
AfiI CCNNNNNNNGG 2 cut(s) 515, 956
AflIII ACRYGT 2 cut(s) 540, 867
AgeI ACCGGT 1 cut(s) 98
AgsI TTSAA 5 cut(s) 250, 538, 611, 739, 772
AjiI CACGTC 1 cut(s) 543
AjnI CCWGG 2 cut(s) 173, 233
AluBI AGCT 3 cut(s) 290, 344, 467
AluI AGCT 3 cut(s) 290, 344, 467
Alw21I GWGCWC 1 cut(s) 648
Alw26I GTCTC 1 cut(s) 752
AlwI GGATC 2 cut(s) 25, 220
AoxI GGCC 3 cut(s) 309, 720, 875
ApeKI GCWGC 4 cut(s) 134, 162, 290, 464
ApoI RAATTY 1 cut(s) 408
ArsI GACNNNNNNTTYG 2 cut(s) 364, 396
AsiGI ACCGGT 1 cut(s) 98
AspLEI GCGC 2 cut(s) 943, 962
AspS9I GGNCC 1 cut(s) 666
AsuHPI GGTGA 1 cut(s) 770
AsuII TTCGAA 1 cut(s) 796
AvaII GGWCC 1 cut(s) 666
BalI TGGCCA 1 cut(s) 722
BanI GGYRCC 1 cut(s) 959
Bbv12I GWGCWC 1 cut(s) 648
BbvI GCAGC 4 cut(s) 121, 149, 277, 476
BccI CCATC 2 cut(s) 332, 712
BceAI ACGGC 2 cut(s) 69, 973
BciT130I CCWGG 2 cut(s) 175, 235
BciVI GTATCC 1 cut(s) 687
BcoDI GTCTC 1 cut(s) 752
BfaI CTAG 2 cut(s) 120, 686
BfmI CTRYAG 1 cut(s) 135
BfoI RGCGCY 1 cut(s) 963
BfuI GTATCC 1 cut(s) 687
BisI GCNGC 5 cut(s) 135, 163, 291, 465, 585
BlsI GCNGC 5 cut(s) 136, 164, 292, 466, 586
Bme1390I CCNGG 2 cut(s) 175, 235
Bme18I GGWCC 1 cut(s) 666
BmgBI CACGTC 1 cut(s) 543
BmgT120I GGNCC 1 cut(s) 666
BmiI GGNNCC 2 cut(s) 668, 961
BmrFI CCNGG 2 cut(s) 175, 235
BmrI ACTGGG 1 cut(s) 524
BmsI GCATC 3 cut(s) 46, 419, 451
BmuI ACTGGG 1 cut(s) 524
BpmI CTGGAG 2 cut(s) 106, 256
Bpu14I TTCGAA 1 cut(s) 796
BpuEI CTTGAG 1 cut(s) 985
BsaHI GRCGYC 1 cut(s) 960
BsaI GGTCTC 1 cut(s) 752
BsaJI CCNNGG 3 cut(s) 68, 754, 853
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 4 cut(s) 49, 79, 582, 612
Bsc4I CCNNNNNNNGG 2 cut(s) 515, 956
Bse118I RCCGGY 1 cut(s) 98
Bse1I ACTGG 5 cut(s) 89, 519, 711, 723, 841
BseBI CCWGG 2 cut(s) 175, 235
BseDI CCNNGG 3 cut(s) 68, 754, 853
BseGI GGATG 7 cut(s) 37, 232, 259, 434, 466, 899, 987
BseLI CCNNNNNNNGG 2 cut(s) 515, 956
BseMII CTCAG 1 cut(s) 380
BseNI ACTGG 5 cut(s) 89, 519, 711, 723, 841
BseRI GAGGAG 1 cut(s) 740
BseXI GCAGC 4 cut(s) 121, 149, 277, 476
BsgI GTGCAG 1 cut(s) 657
BshFI GGCC 3 cut(s) 311, 722, 877
BshNI GGYRCC 1 cut(s) 959
BshTI ACCGGT 1 cut(s) 98
BsiHKAI GWGCWC 1 cut(s) 648
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 2 cut(s) 555, 652
BslI CCNNNNNNNGG 2 cut(s) 515, 956
BsmAI GTCTC 1 cut(s) 752
BsmFI GGGAC 2 cut(s) 555, 652
BsmI GAATGC 1 cut(s) 730
BsnI GGCC 3 cut(s) 311, 722, 877
Bso31I GGTCTC 1 cut(s) 752
Bsp119I TTCGAA 1 cut(s) 796
Bsp1286I GDGCHC 1 cut(s) 648
Bsp143I GATC 3 cut(s) 17, 212, 1004
BspACI CCGC 5 cut(s) 264, 442, 577, 585, 781
BspANI GGCC 3 cut(s) 311, 722, 877
BspCNI CTCAG 1 cut(s) 379
BspLI GGNNCC 2 cut(s) 668, 961
BspMAI CTGCAG 1 cut(s) 139
BspPI GGATC 2 cut(s) 25, 220
BspT104I TTCGAA 1 cut(s) 796
BspT107I GGYRCC 1 cut(s) 959
BspTNI GGTCTC 1 cut(s) 752
BsrFI RCCGGY 1 cut(s) 98
BsrI ACTGG 5 cut(s) 89, 519, 711, 723, 841
BssAI RCCGGY 1 cut(s) 98
BssECI CCNNGG 3 cut(s) 68, 754, 853
BssMI GATC 3 cut(s) 17, 212, 1004
BssNI GRCGYC 1 cut(s) 960
BssT1I CCWWGG 2 cut(s) 68, 754
Bst2UI CCWGG 2 cut(s) 175, 235
Bst4CI ACNGT 2 cut(s) 208, 857
Bst6I CTCTTC 2 cut(s) 181, 480
BstACI GRCGYC 1 cut(s) 960
BstBI TTCGAA 1 cut(s) 796
BstC8I GCNNGC 1 cut(s) 779
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 1 cut(s) 853
BstF5I GGATG 7 cut(s) 37, 232, 259, 434, 466, 899, 987
BstH2I RGCGCY 1 cut(s) 963
BstHHI GCGC 2 cut(s) 943, 962
BstKTI GATC 3 cut(s) 20, 215, 1007
BstMAI GTCTC 1 cut(s) 752
BstMBI GATC 3 cut(s) 17, 212, 1004
BstMWI GCNNNNNNNGC 1 cut(s) 62
BstNI CCWGG 2 cut(s) 175, 235
BstNSI RCATGY 2 cut(s) 781, 871
BstSCI CCNGG 2 cut(s) 173, 233
BstSFI CTRYAG 1 cut(s) 135
BstV1I GCAGC 4 cut(s) 121, 149, 277, 476
BstX2I RGATCY 1 cut(s) 212
BstXI CCANNNNNNTGG 1 cut(s) 75
BstYI RGATCY 1 cut(s) 212
BsuI GTATCC 1 cut(s) 687
BsuRI GGCC 3 cut(s) 311, 722, 877
BtgI CCRYGG 1 cut(s) 853
BtrI CACGTC 1 cut(s) 543
BtsCI GGATG 7 cut(s) 37, 232, 259, 434, 466, 899, 987
BtsI GCAGTG 1 cut(s) 645
BtsIMutI CAGTG 5 cut(s) 374, 512, 645, 648, 848
Cac8I GCNNGC 1 cut(s) 779
CfoI GCGC 2 cut(s) 943, 962
Cfr10I RCCGGY 1 cut(s) 98
Cfr13I GGNCC 1 cut(s) 666
Csp6I GTAC 2 cut(s) 280, 495
CspAI ACCGGT 1 cut(s) 98
CviAII CATG 4 cut(s) 46, 778, 868, 951
CviQI GTAC 2 cut(s) 280, 495
DdeI CTNAG 1 cut(s) 366
DinI GGCGCC 1 cut(s) 961
DpnI GATC 3 cut(s) 19, 214, 1006
DpnII GATC 3 cut(s) 17, 212, 1004
EaeI YGGCCR 1 cut(s) 720
Eam1104I CTCTTC 2 cut(s) 181, 480
EarI CTCTTC 2 cut(s) 181, 480
Eco130I CCWWGG 2 cut(s) 68, 754
Eco31I GGTCTC 1 cut(s) 752
Eco47I GGWCC 1 cut(s) 666
EcoRII CCWGG 2 cut(s) 173, 233
EcoT14I CCWWGG 2 cut(s) 68, 754
EcoT22I ATGCAT 1 cut(s) 732
EgeI GGCGCC 1 cut(s) 961
EheI GGCGCC 1 cut(s) 961
ErhI CCWWGG 2 cut(s) 68, 754
FaeI CATG 4 cut(s) 49, 781, 871, 954
FaqI GGGAC 2 cut(s) 555, 652
FatI CATG 4 cut(s) 45, 777, 867, 950
FauI CCCGC 2 cut(s) 271, 584
Fnu4HI GCNGC 5 cut(s) 135, 163, 291, 465, 585
FokI GGATG 7 cut(s) 24, 239, 246, 441, 473, 886, 994
Fsp4HI GCNGC 5 cut(s) 135, 163, 291, 465, 585
FspBI CTAG 2 cut(s) 120, 686
GlaI GCGC 2 cut(s) 942, 961
GluI GCNGC 5 cut(s) 135, 163, 291, 465, 585
GsuI CTGGAG 2 cut(s) 106, 256
HaeII RGCGCY 1 cut(s) 963
HaeIII GGCC 3 cut(s) 311, 722, 877
HapII CCGG 1 cut(s) 99
HhaI GCGC 2 cut(s) 943, 962
Hin1I GRCGYC 1 cut(s) 960
Hin1II CATG 4 cut(s) 49, 781, 871, 954
Hin6I GCGC 2 cut(s) 941, 960
HinP1I GCGC 2 cut(s) 941, 960
HinfI GANTC 1 cut(s) 798
HpaII CCGG 1 cut(s) 99
HphI GGTGA 1 cut(s) 770
Hpy166II GTNNAC 1 cut(s) 372
Hpy188I TCNGA 2 cut(s) 624, 935
Hpy188III TCNNGA 7 cut(s) 94, 335, 686, 832, 890, 1002, 1012
Hpy8I GTNNAC 1 cut(s) 372
HpyAV CCTTC 3 cut(s) 152, 485, 680
HpyCH4III ACNGT 2 cut(s) 208, 857
HpyCH4IV ACGT 1 cut(s) 542
HpyCH4V TGCA 7 cut(s) 104, 137, 165, 464, 638, 730, 911
HpyF10VI GCNNNNNNNGC 1 cut(s) 62
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 542
Hsp92I GRCGYC 1 cut(s) 960
Hsp92II CATG 4 cut(s) 49, 781, 871, 954
HspAI GCGC 2 cut(s) 941, 960
KasI GGCGCC 1 cut(s) 959
Kzo9I GATC 3 cut(s) 17, 212, 1004
LmnI GCTCC 3 cut(s) 70, 341, 511
Lsp1109I GCAGC 4 cut(s) 121, 149, 277, 476
LweI GCATC 3 cut(s) 46, 419, 451
MaeI CTAG 2 cut(s) 120, 686
MaeII ACGT 1 cut(s) 542
MaeIII GTNAC 1 cut(s) 385
MalI GATC 3 cut(s) 19, 214, 1006
MboI GATC 3 cut(s) 17, 212, 1004
MboII GAAGA 5 cut(s) 198, 349, 497, 539, 575
MflI RGATCY 1 cut(s) 212
MhlI GDGCHC 1 cut(s) 648
MlsI TGGCCA 1 cut(s) 722
MluCI AATT 7 cut(s) 255, 318, 325, 408, 765, 904, 972
MluNI TGGCCA 1 cut(s) 722
Mly113I GGCGCC 1 cut(s) 960
MmeI TCCRAC 2 cut(s) 533, 913
MnlI CCTC 8 cut(s) 81, 171, 217, 231, 451, 481, 583, 761
Mox20I TGGCCA 1 cut(s) 722
Mph1103I ATGCAT 1 cut(s) 732
MscI TGGCCA 1 cut(s) 722
MseI TTAA 2 cut(s) 11, 971
MslI CAYNNNNRTG 1 cut(s) 916
Msp20I TGGCCA 1 cut(s) 722
MspA1I CMGCKG 1 cut(s) 467
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 175, 235
Mva1269I GAATGC 1 cut(s) 730
MvaI CCWGG 2 cut(s) 175, 235
MwoI GCNNNNNNNGC 1 cut(s) 62
NarI GGCGCC 1 cut(s) 960
NdeII GATC 3 cut(s) 17, 212, 1004
NlaIII CATG 4 cut(s) 49, 781, 871, 954
NlaIV GGNNCC 2 cut(s) 668, 961
NmuCI GTSAC 1 cut(s) 385
NsiI ATGCAT 1 cut(s) 732
NspI RCATGY 2 cut(s) 781, 871
NspV TTCGAA 1 cut(s) 796
PaeI GCATGC 1 cut(s) 781
PciI ACATGT 1 cut(s) 867
PcsI WCGNNNNNNNCGW 1 cut(s) 29
PctI GAATGC 1 cut(s) 730
PfeI GAWTC 1 cut(s) 798
PfoI TCCNGGA 1 cut(s) 173
PinAI ACCGGT 1 cut(s) 98
PkrI GCNGC 5 cut(s) 136, 164, 292, 466, 586
PluTI GGCGCC 1 cut(s) 963
PscI ACATGT 1 cut(s) 867
PsiI TTATAA 1 cut(s) 146
Psp6I CCWGG 2 cut(s) 173, 233
PspGI CCWGG 2 cut(s) 173, 233
PspN4I GGNNCC 2 cut(s) 668, 961
PspPI GGNCC 1 cut(s) 666
PstI CTGCAG 1 cut(s) 139
PsuI RGATCY 1 cut(s) 212
PvuII CAGCTG 1 cut(s) 467
RsaI GTAC 2 cut(s) 281, 496
RsaNI GTAC 2 cut(s) 280, 495
RseI CAYNNNNRTG 1 cut(s) 916
SaqAI TTAA 2 cut(s) 11, 971
SatI GCNGC 5 cut(s) 135, 163, 291, 465, 585
Sau3AI GATC 3 cut(s) 17, 212, 1004
Sau96I GGNCC 1 cut(s) 666
ScrFI CCNGG 2 cut(s) 175, 235
SduI GDGCHC 1 cut(s) 648
SetI ASST 7 cut(s) 74, 92, 292, 346, 469, 496, 545
SfaNI GCATC 3 cut(s) 46, 419, 451
SfcI CTRYAG 1 cut(s) 135
SfoI GGCGCC 1 cut(s) 961
SfuI TTCGAA 1 cut(s) 796
SinI GGWCC 1 cut(s) 666
SmiMI CAYNNNNRTG 1 cut(s) 916
SmlI CTYRAG 1 cut(s) 1000
SmoI CTYRAG 1 cut(s) 1000
SphI GCATGC 1 cut(s) 781
Sse9I AATT 7 cut(s) 255, 318, 325, 408, 765, 904, 972
SsiI CCGC 5 cut(s) 264, 442, 577, 585, 781
SspDI GGCGCC 1 cut(s) 959
SspI AATATT 1 cut(s) 108
SspMI CTAG 2 cut(s) 120, 686
StyD4I CCNGG 2 cut(s) 173, 233
StyI CCWWGG 2 cut(s) 68, 754
TaaI ACNGT 2 cut(s) 208, 857
TaiI ACGT 1 cut(s) 545
TaqI TCGA 3 cut(s) 23, 796, 891
TasI AATT 7 cut(s) 255, 318, 325, 408, 765, 904, 972
TauI GCSGC 1 cut(s) 587
TfiI GAWTC 1 cut(s) 798
Tru1I TTAA 2 cut(s) 11, 971
Tru9I TTAA 2 cut(s) 11, 971
TscAI CASTG 5 cut(s) 374, 519, 645, 655, 848
TseFI GTSAC 1 cut(s) 385
TseI GCWGC 4 cut(s) 134, 162, 290, 464
Tsp45I GTSAC 1 cut(s) 385
TspDTI ATGAA 9 cut(s) 171, 243, 248, 319, 498, 540, 576, 723, 998
TspRI CASTG 5 cut(s) 374, 519, 645, 655, 848
VpaK11BI GGWCC 1 cut(s) 666
XapI RAATTY 1 cut(s) 408
XbaI TCTAGA 1 cut(s) 685
XceI RCATGY 2 cut(s) 781, 871
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
XspI CTAG 2 cut(s) 120, 686
Zsp2I ATGCAT 1 cut(s) 732
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.