Rh5AG249300

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000967
Physical Location & Seq
Forward (+)
31536930 .. 31538415
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG249300.1

Sequence Viewer

Length: 489 bp
ATGGCCACTCTTTCTTGGAAAACCCATCACCCCCATCTCTTCCTTTATATACCCCCTCCCATATATCTCTCTCCCCAACAAACATACCCTAATTCTCTCTTTCTCTCTCTTTCATCTCCCAAATCGTCAATGGCTTCTCCTCAGCAAAACGCACAAGCAAATTTCCAAGACTTGCTGCCCACCATGGCCGACAAGCTCGGCGGGGACGGTTTGATCGGCGAGCTCTGTAATGGGTTCAATCTTCTGATGGATCCCGCCAAAGGGGTCATCACCTTCGATAGCCTCAAGAGGAACTCAGCCCTGCTGGGCTTGCAGGACCTGGGCGACGACGATCTACGGTCTATGTTGCAGGAAGGCGATTTCGACGGCGATGGGGCGCTAAATCAGATGGAATTTTGCGTGTTGATGTTTAGATTGAGTCCCGAGCTTATGGAGGAGTCTCGGCTCTGGCTGGACGATGCTCTTCAATACGAGTTTAAGCATTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03420 map03420
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

162

Amino Acids

18.14

Weight (kDa)

4.49

Isoelectric Point (pI)

54.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 87 - 138 1.9e-20 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 201, 255
AclWI GGATC 2 cut(s) 245, 258
AcoI YGGCCR 2 cut(s) 3, 186
AcsI RAATTY 2 cut(s) 160, 392
AfiI CCNNNNNNNGG 2 cut(s) 260, 261
AgsI TTSAA 2 cut(s) 238, 467
AjnI CCWGG 1 cut(s) 318
AluBI AGCT 3 cut(s) 196, 223, 427
AluI AGCT 3 cut(s) 196, 223, 427
Alw21I GWGCWC 1 cut(s) 225
Alw26I GTCTC 1 cut(s) 444
AlwI GGATC 2 cut(s) 245, 258
AlwNI CAGNNNCTG 1 cut(s) 319
Ama87I CYCGRG 1 cut(s) 422
AoxI GGCC 2 cut(s) 3, 186
ApeKI GCWGC 1 cut(s) 175
ApoI RAATTY 2 cut(s) 160, 392
AspLEI GCGC 1 cut(s) 379
AspS9I GGNCC 1 cut(s) 316
AsuHPI GGTGA 2 cut(s) 20, 262
AvaI CYCGRG 1 cut(s) 422
AvaII GGWCC 1 cut(s) 316
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 250
BanII GRGCYC 1 cut(s) 225
Bbv12I GWGCWC 1 cut(s) 225
BbvCI CCTCAGC 1 cut(s) 141
BbvI GCAGC 1 cut(s) 162
BccI CCATC 5 cut(s) 33, 42, 241, 365, 382
BceAI ACGGC 1 cut(s) 382
BciT130I CCWGG 1 cut(s) 320
BcoDI GTCTC 1 cut(s) 444
BfoI RGCGCY 1 cut(s) 380
BisI GCNGC 1 cut(s) 176
BlsI GCNGC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 320
Bme18I GGWCC 1 cut(s) 316
BmeT110I CYCGRG 1 cut(s) 422
BmgT120I GGNCC 1 cut(s) 316
BmiI GGNNCC 1 cut(s) 252
BmrFI CCNGG 1 cut(s) 320
BmsI GCATC 1 cut(s) 448
Bpu10I CCTNAGC 1 cut(s) 141
BpuEI CTTGAG 1 cut(s) 269
BsaJI CCNNGG 2 cut(s) 183, 319
Bsc4I CCNNNNNNNGG 2 cut(s) 260, 261
Bse3DI GCAATG 1 cut(s) 481
BseBI CCWGG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 183, 319
BseLI CCNNNNNNNGG 2 cut(s) 260, 261
BseMI GCAATG 1 cut(s) 481
BseMII CTCAG 2 cut(s) 155, 309
BseRI GAGGAG 2 cut(s) 129, 449
BseXI GCAGC 1 cut(s) 162
BseYI CCCAGC 1 cut(s) 304
BshFI GGCC 2 cut(s) 5, 188
BsiHKAI GWGCWC 1 cut(s) 225
BsiHKCI CYCGRG 1 cut(s) 422
BslFI GGGAC 2 cut(s) 218, 405
BslI CCNNNNNNNGG 2 cut(s) 260, 261
BsmAI GTCTC 1 cut(s) 444
BsmFI GGGAC 2 cut(s) 218, 405
BsnI GGCC 2 cut(s) 5, 188
BsoBI CYCGRG 1 cut(s) 422
Bsp1286I GDGCHC 1 cut(s) 225
Bsp143I GATC 3 cut(s) 213, 250, 331
Bsp19I CCATGG 1 cut(s) 183
BspACI CCGC 2 cut(s) 201, 255
BspANI GGCC 2 cut(s) 5, 188
BspCNI CTCAG 2 cut(s) 154, 308
BspLI GGNNCC 1 cut(s) 252
BspPI GGATC 2 cut(s) 245, 258
BspQI GCTCTTC 1 cut(s) 468
BsrDI GCAATG 1 cut(s) 481
BssECI CCNNGG 2 cut(s) 183, 319
BssMI GATC 3 cut(s) 213, 250, 331
BssT1I CCWWGG 1 cut(s) 183
Bst2UI CCWGG 1 cut(s) 320
Bst4CI ACNGT 2 cut(s) 209, 339
Bst6I CTCTTC 2 cut(s) 44, 468
BstC8I GCNNGC 2 cut(s) 221, 311
BstDEI CTNAG 2 cut(s) 141, 295
BstDSI CCRYGG 1 cut(s) 183
BstH2I RGCGCY 1 cut(s) 380
BstHHI GCGC 1 cut(s) 379
BstKTI GATC 3 cut(s) 216, 253, 334
BstMAI GTCTC 1 cut(s) 444
BstMBI GATC 3 cut(s) 213, 250, 331
BstMWI GCNNNNNNNGC 1 cut(s) 310
BstNI CCWGG 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 318
BstV1I GCAGC 1 cut(s) 162
BstX2I RGATCY 1 cut(s) 250
BstYI RGATCY 1 cut(s) 250
BsuRI GGCC 2 cut(s) 5, 188
BtgI CCRYGG 1 cut(s) 183
BtgZI GCGATG 1 cut(s) 384
Cac8I GCNNGC 2 cut(s) 221, 311
CaiI CAGNNNCTG 1 cut(s) 319
CfoI GCGC 1 cut(s) 379
Cfr13I GGNCC 1 cut(s) 316
CviAII CATG 1 cut(s) 184
DdeI CTNAG 2 cut(s) 141, 295
DpnI GATC 3 cut(s) 215, 252, 333
DpnII GATC 3 cut(s) 213, 250, 331
EaeI YGGCCR 2 cut(s) 3, 186
Eam1104I CTCTTC 2 cut(s) 44, 468
EarI CTCTTC 2 cut(s) 44, 468
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 183
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 316
Eco53kI GAGCTC 1 cut(s) 223
Eco88I CYCGRG 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 183
EcoT38I GRGCYC 1 cut(s) 225
ErhI CCWWGG 1 cut(s) 183
FaeI CATG 1 cut(s) 187
FaiI YATR 8 cut(s) 48, 50, 62, 64, 85, 185, 344, 431
FaqI GGGAC 2 cut(s) 218, 405
FatI CATG 1 cut(s) 183
FauI CCCGC 2 cut(s) 194, 262
Fnu4HI GCNGC 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 1 cut(s) 176
GlaI GCGC 1 cut(s) 378
GluI GCNGC 1 cut(s) 176
GsaI CCCAGC 1 cut(s) 308
HaeII RGCGCY 1 cut(s) 380
HaeIII GGCC 2 cut(s) 5, 188
HhaI GCGC 1 cut(s) 379
Hin1II CATG 1 cut(s) 187
Hin6I GCGC 1 cut(s) 377
HinP1I GCGC 1 cut(s) 377
HinfI GANTC 2 cut(s) 418, 437
HphI GGTGA 2 cut(s) 20, 262
Hpy188I TCNGA 2 cut(s) 246, 387
Hpy188III TCNNGA 2 cut(s) 286, 422
Hpy99I CGWCG 3 cut(s) 329, 332, 368
HpyAV CCTTC 2 cut(s) 283, 347
HpyCH4III ACNGT 2 cut(s) 209, 339
HpyCH4V TGCA 2 cut(s) 313, 349
HpyF10VI GCNNNNNNNGC 1 cut(s) 310
HpyF3I CTNAG 2 cut(s) 141, 295
Hsp92II CATG 1 cut(s) 187
HspAI GCGC 1 cut(s) 377
Kzo9I GATC 3 cut(s) 213, 250, 331
LguI GCTCTTC 1 cut(s) 468
LpnPI CCDG 8 cut(s) 290, 299, 305, 314, 332, 335, 433, 437
Lsp1109I GCAGC 1 cut(s) 162
LweI GCATC 1 cut(s) 448
MalI GATC 3 cut(s) 215, 252, 333
MboI GATC 3 cut(s) 213, 250, 331
MboII GAAGA 3 cut(s) 31, 233, 455
MflI RGATCY 1 cut(s) 250
MhlI GDGCHC 1 cut(s) 225
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 91, 160, 392
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 427, 446
MnlI CCTC 5 cut(s) 66, 150, 282, 293, 427
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 477
Msp20I TGGCCA 1 cut(s) 5
MspR9I CCNGG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 320
MwoI GCNNNNNNNGC 1 cut(s) 310
NcoI CCATGG 1 cut(s) 183
NdeII GATC 3 cut(s) 213, 250, 331
NlaIII CATG 1 cut(s) 187
NlaIV GGNNCC 1 cut(s) 252
NmeAIII GCCGAG 2 cut(s) 177, 421
PciSI GCTCTTC 1 cut(s) 468
PkrI GCNGC 1 cut(s) 177
PleI GAGTC 2 cut(s) 426, 445
PpsI GAGTC 2 cut(s) 426, 445
PpuMI RGGWCCY 1 cut(s) 316
Psp124BI GAGCTC 1 cut(s) 225
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 318
PspFI CCCAGC 1 cut(s) 304
PspGI CCWGG 1 cut(s) 318
PspN4I GGNNCC 1 cut(s) 252
PspPI GGNCC 1 cut(s) 316
PspPPI RGGWCCY 1 cut(s) 316
PstNI CAGNNNCTG 1 cut(s) 319
PsuI RGATCY 1 cut(s) 250
SacI GAGCTC 1 cut(s) 225
SapI GCTCTTC 1 cut(s) 468
SaqAI TTAA 1 cut(s) 477
SatI GCNGC 1 cut(s) 176
Sau3AI GATC 3 cut(s) 213, 250, 331
Sau96I GGNCC 1 cut(s) 316
SchI GAGTC 2 cut(s) 427, 446
ScrFI CCNGG 1 cut(s) 320
SduI GDGCHC 1 cut(s) 225
SetI ASST 5 cut(s) 198, 225, 275, 321, 429
SfaNI GCATC 1 cut(s) 448
SinI GGWCC 1 cut(s) 316
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
Sse9I AATT 3 cut(s) 91, 160, 392
SsiI CCGC 2 cut(s) 201, 255
SstI GAGCTC 1 cut(s) 225
StyD4I CCNGG 1 cut(s) 318
StyI CCWWGG 1 cut(s) 183
TaaI ACNGT 2 cut(s) 209, 339
TaqI TCGA 2 cut(s) 276, 363
TasI AATT 3 cut(s) 91, 160, 392
Tru1I TTAA 1 cut(s) 477
Tru9I TTAA 1 cut(s) 477
TseI GCWGC 1 cut(s) 175
TspDTI ATGAA 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 316
XapI RAATTY 2 cut(s) 160, 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.