MD05G1329200.v1.1

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0000967
Physical Location & Seq
Reverse (-)
45426660 .. 45426998
339 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1329200.v1.1.491

Sequence Viewer

Length: 339 bp
ATGGCTTTTCGAAATGGGGTTATTGTGTTTGAAGATTTCTTTCCAGCCATGGTTGAGAAACTGGGAGCAGAAGGGTTTATGGAGGAGCTGTCGAAAGGGTTTCGGTTGTTGACGGATGGAGAGAAGGGAGTGATCACATTTGAGAGCTTGAAGAAGAACTCGGGATTGCTTGGATTGCAAGGCATGAGCGATGAGGATATCATGTGCATGCTCAAGGAAGGTGATTTGGACGGTGATGGCGCTTTGGATGAGATGGAGTTTTGCACCCTCATGTTCAGATTGAGCCCTGCCATGATGCAAAGTTCTCAAGAATTATTGGTGGAAGCTTTAGATATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

113

Amino Acids

12.4

Weight (kDa)

4.14

Isoelectric Point (pI)

42.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 41 - 93 1.6e-18 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AgsI TTSAA 2 cut(s) 32, 151
AluBI AGCT 3 cut(s) 88, 147, 326
AluI AGCT 3 cut(s) 88, 147, 326
Ama87I CYCGRG 1 cut(s) 160
AspLEI GCGC 1 cut(s) 242
AsuHPI GGTGA 2 cut(s) 233, 245
AsuII TTCGAA 1 cut(s) 10
AvaI CYCGRG 1 cut(s) 160
BanII GRGCYC 1 cut(s) 287
BccI CCATC 3 cut(s) 110, 230, 247
BclI TGATCA 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 243
BmeT110I CYCGRG 1 cut(s) 160
BmrI ACTGGG 1 cut(s) 71
BmsI GCATC 1 cut(s) 285
BmuI ACTGGG 1 cut(s) 71
Bpu14I TTCGAA 1 cut(s) 10
BpuEI CTTGAG 2 cut(s) 197, 291
BsaJI CCNNGG 1 cut(s) 48
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse1I ACTGG 1 cut(s) 66
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 2 cut(s) 121, 253
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 160
BsoBI CYCGRG 1 cut(s) 160
Bsp119I TTCGAA 1 cut(s) 10
Bsp1286I GDGCHC 1 cut(s) 287
Bsp143I GATC 1 cut(s) 132
Bsp19I CCATGG 1 cut(s) 48
BspT104I TTCGAA 1 cut(s) 10
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 1 cut(s) 132
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 1 cut(s) 233
BstBI TTCGAA 1 cut(s) 10
BstC8I GCNNGC 1 cut(s) 209
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 2 cut(s) 121, 253
BstH2I RGCGCY 1 cut(s) 243
BstHHI GCGC 1 cut(s) 242
BstKTI GATC 1 cut(s) 135
BstMBI GATC 1 cut(s) 132
BstMWI GCNNNNNNNGC 1 cut(s) 175
BstNSI RCATGY 1 cut(s) 211
BtgI CCRYGG 1 cut(s) 48
BtgZI GCGATG 1 cut(s) 204
BtsCI GGATG 2 cut(s) 121, 253
Cac8I GCNNGC 1 cut(s) 209
CfoI GCGC 1 cut(s) 242
CviAII CATG 6 cut(s) 49, 184, 202, 208, 271, 292
CviJI RGCY 6 cut(s) 5, 47, 88, 147, 285, 326
CviKI_1 RGCY 6 cut(s) 5, 47, 88, 147, 285, 326
DpnI GATC 1 cut(s) 134
DpnII GATC 1 cut(s) 132
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 1 cut(s) 287
Eco32I GATATC 1 cut(s) 199
Eco88I CYCGRG 1 cut(s) 160
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 6 cut(s) 52, 187, 205, 211, 274, 295
FaiI YATR 7 cut(s) 50, 80, 185, 203, 209, 272, 293
FatI CATG 6 cut(s) 48, 183, 201, 207, 270, 291
FbaI TGATCA 1 cut(s) 132
FokI GGATG 2 cut(s) 128, 260
FriOI GRGCYC 1 cut(s) 287
GlaI GCGC 1 cut(s) 241
HaeII RGCGCY 1 cut(s) 243
HhaI GCGC 1 cut(s) 242
Hin1II CATG 6 cut(s) 52, 187, 205, 211, 274, 295
Hin6I GCGC 1 cut(s) 240
HinP1I GCGC 1 cut(s) 240
HincII GTYRAC 1 cut(s) 111
HindII GTYRAC 1 cut(s) 111
HindIII AAGCTT 1 cut(s) 324
HphI GGTGA 2 cut(s) 233, 245
Hpy166II GTNNAC 1 cut(s) 111
Hpy188I TCNGA 1 cut(s) 278
Hpy188III TCNNGA 2 cut(s) 162, 308
Hpy8I GTNNAC 1 cut(s) 111
HpyAV CCTTC 3 cut(s) 65, 118, 212
HpyCH4III ACNGT 1 cut(s) 233
HpyCH4V TGCA 4 cut(s) 178, 207, 264, 298
HpyF10VI GCNNNNNNNGC 1 cut(s) 175
Hsp92II CATG 6 cut(s) 52, 187, 205, 211, 274, 295
HspAI GCGC 1 cut(s) 240
Ksp22I TGATCA 1 cut(s) 132
Kzo9I GATC 1 cut(s) 132
LmnI GCTCC 2 cut(s) 65, 85
LpnPI CCDG 3 cut(s) 47, 57, 300
LweI GCATC 1 cut(s) 285
MalI GATC 1 cut(s) 134
MboI GATC 1 cut(s) 132
MboII GAAGA 3 cut(s) 44, 163, 166
MhlI GDGCHC 1 cut(s) 287
MluCI AATT 1 cut(s) 311
MnlI CCTC 3 cut(s) 76, 187, 278
MseI TTAA 1 cut(s) 337
MslI CAYNNNNRTG 2 cut(s) 206, 269
MwoI GCNNNNNNNGC 1 cut(s) 175
NcoI CCATGG 1 cut(s) 48
NdeII GATC 1 cut(s) 132
NlaIII CATG 6 cut(s) 52, 187, 205, 211, 274, 295
NspI RCATGY 1 cut(s) 211
NspV TTCGAA 1 cut(s) 10
PaeI GCATGC 1 cut(s) 211
RseI CAYNNNNRTG 2 cut(s) 206, 269
SaqAI TTAA 1 cut(s) 337
Sau3AI GATC 1 cut(s) 132
SduI GDGCHC 1 cut(s) 287
SetI ASST 4 cut(s) 90, 149, 223, 328
SfaNI GCATC 1 cut(s) 285
SfuI TTCGAA 1 cut(s) 10
SmiMI CAYNNNNRTG 2 cut(s) 206, 269
SmlI CTYRAG 2 cut(s) 212, 306
SmoI CTYRAG 2 cut(s) 212, 306
SphI GCATGC 1 cut(s) 211
Sse9I AATT 1 cut(s) 311
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 1 cut(s) 233
TaqI TCGA 2 cut(s) 10, 92
TasI AATT 1 cut(s) 311
Tru1I TTAA 1 cut(s) 337
Tru9I TTAA 1 cut(s) 337
TspGWI ACGGA 1 cut(s) 128
XceI RCATGY 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.