Rorug02G0485800

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000967
Physical Location & Seq
Reverse (-)
61556045 .. 61558364
2320 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0485800.1

Sequence Viewer

Length: 1077 bp
ATGATCGTCAATTTGCTTTACAGCTTCTATCATACTTCTTCTAGAGTACATCTTCGATCAGACAAATTAGTGATGATGGAAATTCTAGTTCATCATATGATATTCATTTTGCTGTTATCCATATGCAGCGCAAGTACTGCTGCACCACGGATGGCAATAGGTGATTGCCCAACGAAGTGTGGAAATGTTATCATCCCGTACCCTTTCGGGATTGGACCTAATCCAAGTTGTTACTATGACGAATGGTTTCAAATCGACTGCAACGAGGAGAAGCCTTTCTTGAGGCGTACCAAATTGGAGGTGGTGAATATATCTACCGAAGGATGGGTTCAGGTGTACAGCCCCGTTACATTCTTCTGCGAGGGAAATAATAGTAGCCAACTGGCGGATTTAAGGGACAGCCCTTTCTTCTACTCTCGAAGATACAACATATTCACTGCAGTGAGTTGCGATTCCTCTGCCCTGTTGAGTTCCGCAGACTCTGTTGTTGGCGGATGCAGGTCAGTGTGTGATCAATCTGGAGACCTGACTTGCGATATCGGTATTAACTGTTGTCAGATTAGTATTCCTCCATACCTCAGTGTTATTAATACTACCTTAGATTATTCAAAAAAAAATACTACCTTAGATGAGACTACTACAAATTGCACCAAGTTTGCCTTCCTGGTTGCCAAAGATTGGGCGGAGAGAACCATCAATTTATCAACAATTTCGGGCCTTACAGCTGTCTTGAAGAGGATGGGCAAGGTTTCTGTGTCACTAAGATGGAGGATTATCAACGACGCTAGGTCATTTGAATTATTTAACCAGTTCAATTTTTCAACGCTTCAGGTCGACCCGGCTTGCTCTATTAGCACTTATCTCGCAGTCAAACGGTCGGAAATTTATTGTTCATGCCGCAAGGGGTTCGAAGGAAATCCGTATCTCGGGGAAAAATCTTGTGATGTAGATATTAATGAATGTCAAGATGGTCGCCGCCTATGCTTTGGCAACAGCAGGTGCGTGAACGAGTATGGTTCCTACCGATGTGAGGACGACAAGAGAGGCCTTGCCGCAGGTAGAGAATATAATTCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03420 map03420
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

358

Amino Acids

40.2

Weight (kDa)

6.14

Isoelectric Point (pI)

51.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 56 - 108 2.2e-12 Wall-associated receptor kinase galacturonan-binding
EGF_CA PF07645 317 - 344 7.1e-07 Calcium-binding EGF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 987
Acc36I ACCTGC 3 cut(s) 489, 987, 1046
AccB7I CCANNNNNTGG 1 cut(s) 678
AccI GTMKAC 1 cut(s) 834
AciI CCGC 7 cut(s) 386, 474, 492, 683, 898, 976, 1053
AcsI RAATTY 2 cut(s) 81, 882
AcuI CTGAAG 1 cut(s) 812
AfaI GTAC 5 cut(s) 48, 136, 200, 289, 338
AfiI CCNNNNNNNGG 5 cut(s) 324, 385, 678, 926, 1030
AgsI TTSAA 6 cut(s) 251, 609, 733, 797, 814, 822
AhdI GACNNNNNGTC 1 cut(s) 787
AjnI CCWGG 1 cut(s) 663
AjuI GAANNNNNNNTTGG 2 cut(s) 644, 676
AleI CACNNNNGTG 1 cut(s) 440
AluBI AGCT 2 cut(s) 24, 725
AluI AGCT 2 cut(s) 24, 725
Alw26I GTCTC 2 cut(s) 516, 626
AlwNI CAGNNNCTG 1 cut(s) 482
Ama87I CYCGRG 1 cut(s) 926
AoxI GGCC 2 cut(s) 715, 1045
ApeKI GCWGC 2 cut(s) 126, 140
ApoI RAATTY 2 cut(s) 81, 882
AseI ATTAAT 2 cut(s) 588, 954
Asp700I GAANNNNTTC 2 cut(s) 246, 275
AspLEI GCGC 1 cut(s) 131
AspS9I GGNCC 2 cut(s) 215, 715
AsuC2I CCSGG 1 cut(s) 839
AsuHPI GGTGA 2 cut(s) 173, 316
AsuII TTCGAA 1 cut(s) 909
AvaI CYCGRG 1 cut(s) 926
AvaII GGWCC 1 cut(s) 215
BbvI GCAGC 2 cut(s) 127, 138
BccI CCATC 7 cut(s) 70, 145, 318, 701, 733, 759, 962
BcgI CGANNNNNNTGC 6 cut(s) 440, 474, 844, 878, 889, 923
BciT130I CCWGG 1 cut(s) 665
BclI TGATCA 1 cut(s) 511
BcnI CCSGG 1 cut(s) 839
BcoDI GTCTC 2 cut(s) 516, 626
BfaI CTAG 3 cut(s) 42, 86, 786
BfmI CTRYAG 1 cut(s) 438
BfuAI ACCTGC 3 cut(s) 489, 987, 1046
BisI GCNGC 5 cut(s) 127, 141, 898, 976, 1053
BlsI GCNGC 5 cut(s) 128, 142, 899, 977, 1054
BmcAI AGTACT 1 cut(s) 136
Bme1390I CCNGG 2 cut(s) 665, 839
Bme18I GGWCC 1 cut(s) 215
BmeRI GACNNNNNGTC 1 cut(s) 787
BmeT110I CYCGRG 1 cut(s) 926
BmgT120I GGNCC 2 cut(s) 215, 715
BmiI GGNNCC 1 cut(s) 1018
BmrFI CCNGG 2 cut(s) 665, 839
BmsI GCATC 1 cut(s) 485
BpmI CTGGAG 1 cut(s) 540
Bpu14I TTCGAA 1 cut(s) 909
BpuEI CTTGAG 1 cut(s) 301
BpuMI CCSGG 1 cut(s) 839
BsaI GGTCTC 1 cut(s) 516
BsaJI CCNNGG 1 cut(s) 146
Bsc4I CCNNNNNNNGG 5 cut(s) 324, 385, 678, 926, 1030
Bse1I ACTGG 2 cut(s) 387, 808
BseBI CCWGG 1 cut(s) 665
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 5 cut(s) 156, 192, 329, 500, 744
BseLI CCNNNNNNNGG 5 cut(s) 324, 385, 678, 926, 1030
BseMII CTCAG 1 cut(s) 592
BseNI ACTGG 2 cut(s) 387, 808
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 2 cut(s) 127, 138
BsgI GTGCAG 1 cut(s) 126
Bsh1285I CGRYCG 1 cut(s) 878
BshFI GGCC 2 cut(s) 717, 1047
BsiEI CGRYCG 1 cut(s) 878
BsiHKCI CYCGRG 1 cut(s) 926
BsiSI CCGG 1 cut(s) 839
BslFI GGGAC 1 cut(s) 410
BslI CCNNNNNNNGG 5 cut(s) 324, 385, 678, 926, 1030
BsmAI GTCTC 2 cut(s) 516, 626
BsmFI GGGAC 1 cut(s) 410
BsnI GGCC 2 cut(s) 717, 1047
Bso31I GGTCTC 1 cut(s) 516
BsoBI CYCGRG 1 cut(s) 926
Bsp119I TTCGAA 1 cut(s) 909
Bsp1407I TGTACA 1 cut(s) 336
Bsp143I GATC 3 cut(s) 3, 56, 511
BspACI CCGC 7 cut(s) 386, 474, 492, 683, 898, 976, 1053
BspANI GGCC 2 cut(s) 717, 1047
BspCNI CTCAG 1 cut(s) 591
BspLI GGNNCC 1 cut(s) 1018
BspMAI CTGCAG 1 cut(s) 442
BspMI ACCTGC 3 cut(s) 489, 987, 1046
BspT104I TTCGAA 1 cut(s) 909
BspTNI GGTCTC 1 cut(s) 516
BsrGI TGTACA 1 cut(s) 336
BsrI ACTGG 2 cut(s) 387, 808
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 3 cut(s) 3, 56, 511
Bst2UI CCWGG 1 cut(s) 665
Bst4CI ACNGT 2 cut(s) 551, 876
Bst6I CTCTTC 1 cut(s) 728
BstAPI GCANNNNNTGC 1 cut(s) 137
BstAUI TGTACA 1 cut(s) 336
BstBI TTCGAA 1 cut(s) 909
BstC8I GCNNGC 1 cut(s) 844
BstDEI CTNAG 4 cut(s) 578, 598, 625, 761
BstDSI CCRYGG 1 cut(s) 146
BstF5I GGATG 5 cut(s) 156, 192, 329, 500, 744
BstHHI GCGC 1 cut(s) 131
BstKTI GATC 3 cut(s) 6, 59, 514
BstMAI GTCTC 2 cut(s) 516, 626
BstMBI GATC 3 cut(s) 3, 56, 511
BstMCI CGRYCG 1 cut(s) 878
BstMWI GCNNNNNNNGC 3 cut(s) 137, 852, 981
BstNI CCWGG 1 cut(s) 665
BstSCI CCNGG 2 cut(s) 663, 837
BstSFI CTRYAG 1 cut(s) 438
BstV1I GCAGC 2 cut(s) 127, 138
BsuRI GGCC 2 cut(s) 717, 1047
BtgI CCRYGG 1 cut(s) 146
BtsCI GGATG 5 cut(s) 156, 192, 329, 500, 744
BtsI GCAGTG 2 cut(s) 435, 447
BtsIMutI CAGTG 4 cut(s) 435, 447, 510, 586
BveI ACCTGC 3 cut(s) 489, 987, 1046
Cac8I GCNNGC 1 cut(s) 844
CaiI CAGNNNCTG 1 cut(s) 482
CfoI GCGC 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 215, 715
CseI GACGC 1 cut(s) 791
Csp6I GTAC 5 cut(s) 47, 135, 199, 288, 337
CviAII CATG 1 cut(s) 894
CviJI RGCY 9 cut(s) 24, 274, 342, 378, 402, 717, 725, 842, 1047
CviKI_1 RGCY 9 cut(s) 24, 274, 342, 378, 402, 717, 725, 842, 1047
CviQI GTAC 5 cut(s) 47, 135, 199, 288, 337
DdeI CTNAG 4 cut(s) 578, 598, 625, 761
DpnI GATC 3 cut(s) 5, 58, 513
DpnII GATC 3 cut(s) 3, 56, 511
DriI GACNNNNNGTC 1 cut(s) 787
Eam1104I CTCTTC 1 cut(s) 728
Eam1105I GACNNNNNGTC 1 cut(s) 787
EarI CTCTTC 1 cut(s) 728
EciI GGCGGA 3 cut(s) 401, 507, 698
Eco147I AGGCCT 1 cut(s) 1047
Eco31I GGTCTC 1 cut(s) 516
Eco32I GATATC 1 cut(s) 538
Eco47I GGWCC 1 cut(s) 215
Eco57I CTGAAG 1 cut(s) 812
Eco88I CYCGRG 1 cut(s) 926
EcoRII CCWGG 1 cut(s) 663
EcoRV GATATC 1 cut(s) 538
FaeI CATG 1 cut(s) 897
FalI AAGNNNNNCTT 4 cut(s) 263, 295, 644, 676
FaqI GGGAC 1 cut(s) 410
FatI CATG 1 cut(s) 893
FauNDI CATATG 2 cut(s) 96, 122
FbaI TGATCA 1 cut(s) 511
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 5 cut(s) 127, 141, 898, 976, 1053
FokI GGATG 5 cut(s) 163, 179, 336, 507, 751
Fsp4HI GCNGC 5 cut(s) 127, 141, 898, 976, 1053
FspBI CTAG 3 cut(s) 42, 86, 786
GlaI GCGC 1 cut(s) 130
GluI GCNGC 5 cut(s) 127, 141, 898, 976, 1053
GsuI CTGGAG 1 cut(s) 540
HaeIII GGCC 2 cut(s) 717, 1047
HapII CCGG 1 cut(s) 839
HgaI GACGC 1 cut(s) 791
HhaI GCGC 1 cut(s) 131
Hin1II CATG 1 cut(s) 897
Hin6I GCGC 1 cut(s) 129
HinP1I GCGC 1 cut(s) 129
HincII GTYRAC 1 cut(s) 835
HindII GTYRAC 1 cut(s) 835
HinfI GANTC 2 cut(s) 452, 479
HpaII CCGG 1 cut(s) 839
HphI GGTGA 2 cut(s) 173, 316
Hpy166II GTNNAC 3 cut(s) 337, 835, 1006
Hpy188I TCNGA 3 cut(s) 61, 558, 880
Hpy188III TCNNGA 7 cut(s) 42, 208, 280, 417, 519, 730, 965
Hpy8I GTNNAC 3 cut(s) 337, 835, 1006
Hpy99I CGWCG 1 cut(s) 785
HpyAV CCTTC 3 cut(s) 314, 670, 905
HpyCH4III ACNGT 2 cut(s) 551, 876
HpyCH4V TGCA 6 cut(s) 126, 143, 261, 440, 498, 648
HpyF10VI GCNNNNNNNGC 3 cut(s) 137, 852, 981
HpyF3I CTNAG 4 cut(s) 578, 598, 625, 761
Hsp92II CATG 1 cut(s) 897
HspAI GCGC 1 cut(s) 129
Ksp22I TGATCA 1 cut(s) 511
Kzo9I GATC 3 cut(s) 3, 56, 511
Lsp1109I GCAGC 2 cut(s) 127, 138
LweI GCATC 1 cut(s) 485
MaeI CTAG 3 cut(s) 42, 86, 786
MaeIII GTNAC 3 cut(s) 230, 346, 756
MalI GATC 3 cut(s) 5, 58, 513
MboI GATC 3 cut(s) 3, 56, 511
MboII GAAGA 6 cut(s) 30, 44, 346, 400, 432, 745
MlyI GAGTC 1 cut(s) 473
MmeI TCCRAC 1 cut(s) 858
MroXI GAANNNNTTC 2 cut(s) 246, 275
MseI TTAA 5 cut(s) 392, 546, 588, 804, 954
MslI CAYNNNNRTG 2 cut(s) 440, 763
MspA1I CMGCKG 1 cut(s) 725
MspI CCGG 1 cut(s) 839
MspR9I CCNGG 2 cut(s) 665, 839
MvaI CCWGG 1 cut(s) 665
MwoI GCNNNNNNNGC 3 cut(s) 137, 852, 981
NciI CCSGG 1 cut(s) 839
NdeI CATATG 2 cut(s) 96, 122
NdeII GATC 3 cut(s) 3, 56, 511
NlaIII CATG 1 cut(s) 897
NlaIV GGNNCC 1 cut(s) 1018
NmuCI GTSAC 1 cut(s) 756
NspV TTCGAA 1 cut(s) 909
OliI CACNNNNGTG 1 cut(s) 440
PaqCI CACCTGC 1 cut(s) 987
PceI AGGCCT 1 cut(s) 1047
PcsI WCGNNNNNNNCGW 1 cut(s) 261
PdmI GAANNNNTTC 2 cut(s) 246, 275
PfeI GAWTC 1 cut(s) 452
PflMI CCANNNNNTGG 1 cut(s) 678
PkrI GCNGC 5 cut(s) 128, 142, 899, 977, 1054
PleI GAGTC 1 cut(s) 473
PpsI GAGTC 1 cut(s) 473
PshBI ATTAAT 2 cut(s) 588, 954
Psp6I CCWGG 1 cut(s) 663
PspGI CCWGG 1 cut(s) 663
PspN4I GGNNCC 1 cut(s) 1018
PspPI GGNCC 2 cut(s) 215, 715
PstI CTGCAG 1 cut(s) 442
PstNI CAGNNNCTG 1 cut(s) 482
PvuII CAGCTG 1 cut(s) 725
RsaI GTAC 5 cut(s) 48, 136, 200, 289, 338
RsaNI GTAC 5 cut(s) 47, 135, 199, 288, 337
RseI CAYNNNNRTG 2 cut(s) 440, 763
SalI GTCGAC 1 cut(s) 833
SaqAI TTAA 5 cut(s) 392, 546, 588, 804, 954
SatI GCNGC 5 cut(s) 127, 141, 898, 976, 1053
Sau3AI GATC 3 cut(s) 3, 56, 511
Sau96I GGNCC 2 cut(s) 215, 715
ScaI AGTACT 1 cut(s) 136
SchI GAGTC 1 cut(s) 473
ScrFI CCNGG 2 cut(s) 665, 839
SfaNI GCATC 1 cut(s) 485
SfcI CTRYAG 1 cut(s) 438
SfuI TTCGAA 1 cut(s) 909
SinI GGWCC 1 cut(s) 215
SmiMI CAYNNNNRTG 2 cut(s) 440, 763
SmlI CTYRAG 1 cut(s) 280
SmoI CTYRAG 1 cut(s) 280
SseBI AGGCCT 1 cut(s) 1047
SsiI CCGC 7 cut(s) 386, 474, 492, 683, 898, 976, 1053
SspMI CTAG 3 cut(s) 42, 86, 786
StuI AGGCCT 1 cut(s) 1047
StyD4I CCNGG 2 cut(s) 663, 837
TaaI ACNGT 2 cut(s) 551, 876
TaqI TCGA 5 cut(s) 55, 255, 418, 834, 909
TatI WGTACW 3 cut(s) 46, 134, 336
TauI GCSGC 3 cut(s) 900, 978, 1055
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 5 cut(s) 392, 546, 588, 804, 954
Tru9I TTAA 5 cut(s) 392, 546, 588, 804, 954
TscAI CASTG 4 cut(s) 442, 447, 510, 586
TseFI GTSAC 1 cut(s) 756
TseI GCWGC 2 cut(s) 126, 140
Tsp45I GTSAC 1 cut(s) 756
TspDTI ATGAA 5 cut(s) 80, 94, 882, 972, 1062
TspGWI ACGGA 2 cut(s) 163, 909
TspRI CASTG 4 cut(s) 442, 447, 510, 586
Van91I CCANNNNNTGG 1 cut(s) 678
VpaK11BI GGWCC 1 cut(s) 215
VspI ATTAAT 2 cut(s) 588, 954
XapI RAATTY 2 cut(s) 81, 882
XbaI TCTAGA 1 cut(s) 41
XcmI CCANNNNNNNNNTGG 1 cut(s) 298
XmiI GTMKAC 1 cut(s) 834
XmnI GAANNNNTTC 2 cut(s) 246, 275
XspI CTAG 3 cut(s) 42, 86, 786
ZrmI AGTACT 1 cut(s) 136
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.