FvH4_3g03600

Calcium-binding protein PBP1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000967
Physical Location & Seq
Forward (+)
2012052 .. 2012596
545 bp
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UTR
Exon/CDS
Intron
FvH4_3g03600.t1

Sequence Viewer

Length: 345 bp
ATGGCGTCGAGAAGTGGCCCGCAGGTTGTGTTTGAGGATTCGTTTCCGACGATGTTAGAGAGGTTAGGAGAGGAAGGGTTTATGAGGGAGTTGTGCAACGGGTTCCGTCTGCTGAAGGACGAGAAGAGAGAGGTGATCACGTTCGAGAGCTTGAAGAGGAACTCGGCGATGCTGGGGTTGCAGGGAATGAGTGATGAAGAGCTGAGGTGCATGGTGAGAGAAGGTGACTTGGATGGAGATGGTTGTCTGAGTGAGATGGAGTTTTGTACTCTCATGTTTAGGTTGAGTCCTGCTTTGATGCAGGTTTCTAAGGAATTGTTGCAGGAGGCTATAGTACGCAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_8

Protein Analysis

115

Amino Acids

12.98

Weight (kDa)

4.66

Isoelectric Point (pI)

63.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_8 PF13833 44 - 94 7.3e-20 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 13, 292
AciI CCGC 1 cut(s) 20
AcuI CTGAAG 1 cut(s) 134
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 268, 336
AgsI TTSAA 1 cut(s) 154
AluBI AGCT 2 cut(s) 150, 202
AluI AGCT 2 cut(s) 150, 202
AoxI GGCC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 17
AsuHPI GGTGA 3 cut(s) 145, 226, 236
BbvCI CCTCAGC 1 cut(s) 203
BccI CCATC 3 cut(s) 227, 233, 250
BclI TGATCA 1 cut(s) 135
BfmI CTRYAG 1 cut(s) 330
BfuAI ACCTGC 2 cut(s) 13, 292
BmgT120I GGNCC 1 cut(s) 17
BmiI GGNNCC 1 cut(s) 104
BmsI GCATC 2 cut(s) 159, 288
Bpu10I CCTNAGC 1 cut(s) 203
BsaHI GRCGYC 1 cut(s) 5
BseGI GGATG 1 cut(s) 238
BseMII CTCAG 2 cut(s) 194, 239
BseYI CCCAGC 1 cut(s) 172
BshFI GGCC 1 cut(s) 18
BsnI GGCC 1 cut(s) 18
Bsp143I GATC 1 cut(s) 135
BspACI CCGC 1 cut(s) 20
BspANI GGCC 1 cut(s) 18
BspCNI CTCAG 2 cut(s) 195, 240
BspLI GGNNCC 1 cut(s) 104
BspMI ACCTGC 2 cut(s) 13, 292
BspQI GCTCTTC 1 cut(s) 192
BssMI GATC 1 cut(s) 135
BssNI GRCGYC 1 cut(s) 5
Bst6I CTCTTC 3 cut(s) 119, 149, 192
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 3 cut(s) 203, 248, 309
BstF5I GGATG 1 cut(s) 238
BstKTI GATC 1 cut(s) 138
BstMBI GATC 1 cut(s) 135
BstMWI GCNNNNNNNGC 1 cut(s) 178
BstSFI CTRYAG 1 cut(s) 330
BsuRI GGCC 1 cut(s) 18
BtgZI GCGATG 1 cut(s) 182
BtsCI GGATG 1 cut(s) 238
BveI ACCTGC 2 cut(s) 13, 292
Cac8I GCNNGC 1 cut(s) 20
Cfr13I GGNCC 1 cut(s) 17
Csp6I GTAC 2 cut(s) 267, 335
CviAII CATG 2 cut(s) 211, 274
CviJI RGCY 4 cut(s) 18, 150, 202, 329
CviKI_1 RGCY 4 cut(s) 18, 150, 202, 329
CviQI GTAC 2 cut(s) 267, 335
DdeI CTNAG 3 cut(s) 203, 248, 309
DpnI GATC 1 cut(s) 137
DpnII GATC 1 cut(s) 135
Eam1104I CTCTTC 3 cut(s) 119, 149, 192
EarI CTCTTC 3 cut(s) 119, 149, 192
Eco57I CTGAAG 1 cut(s) 134
FaeI CATG 2 cut(s) 214, 277
FaiI YATR 4 cut(s) 83, 212, 275, 332
FatI CATG 2 cut(s) 210, 273
FauI CCCGC 1 cut(s) 27
FbaI TGATCA 1 cut(s) 135
FokI GGATG 1 cut(s) 245
GsaI CCCAGC 1 cut(s) 176
HaeIII GGCC 1 cut(s) 18
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 214, 277
HinfI GANTC 2 cut(s) 38, 286
HphI GGTGA 3 cut(s) 145, 226, 236
Hpy188I TCNGA 2 cut(s) 48, 249
Hpy188III TCNNGA 2 cut(s) 9, 145
Hpy99I CGWCG 2 cut(s) 10, 52
HpyAV CCTTC 3 cut(s) 68, 109, 215
HpyCH4IV ACGT 1 cut(s) 140
HpyCH4V TGCA 5 cut(s) 96, 181, 210, 301, 322
HpyF10VI GCNNNNNNNGC 1 cut(s) 178
HpyF3I CTNAG 3 cut(s) 203, 248, 309
HpySE526I ACGT 1 cut(s) 140
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 214, 277
Ksp22I TGATCA 1 cut(s) 135
Kzo9I GATC 1 cut(s) 135
LguI GCTCTTC 1 cut(s) 192
LpnPI CCDG 6 cut(s) 8, 158, 167, 287, 303, 308
LweI GCATC 2 cut(s) 159, 288
MaeII ACGT 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 224
MalI GATC 1 cut(s) 137
MboI GATC 1 cut(s) 135
MboII GAAGA 3 cut(s) 136, 166, 209
MluCI AATT 1 cut(s) 314
MlyI GAGTC 1 cut(s) 295
MmeI TCCRAC 1 cut(s) 71
MnlI CCTC 8 cut(s) 28, 54, 64, 78, 124, 150, 198, 319
MwoI GCNNNNNNNGC 1 cut(s) 178
NdeII GATC 1 cut(s) 135
NlaIII CATG 2 cut(s) 214, 277
NlaIV GGNNCC 1 cut(s) 104
NmeAIII GCCGAG 1 cut(s) 143
NmuCI GTSAC 1 cut(s) 224
PciSI GCTCTTC 1 cut(s) 192
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PfeI GAWTC 1 cut(s) 38
PleI GAGTC 1 cut(s) 294
PpsI GAGTC 1 cut(s) 294
PspFI CCCAGC 1 cut(s) 172
PspN4I GGNNCC 1 cut(s) 104
PspPI GGNCC 1 cut(s) 17
RsaI GTAC 2 cut(s) 268, 336
RsaNI GTAC 2 cut(s) 267, 335
SapI GCTCTTC 1 cut(s) 192
Sau3AI GATC 1 cut(s) 135
Sau96I GGNCC 1 cut(s) 17
SchI GAGTC 1 cut(s) 295
SfaNI GCATC 2 cut(s) 159, 288
SfcI CTRYAG 1 cut(s) 330
Sse9I AATT 1 cut(s) 314
SsiI CCGC 1 cut(s) 20
TaiI ACGT 1 cut(s) 143
TaqI TCGA 2 cut(s) 8, 144
TasI AATT 1 cut(s) 314
TatI WGTACW 1 cut(s) 266
TfiI GAWTC 1 cut(s) 38
TseFI GTSAC 1 cut(s) 224
Tsp45I GTSAC 1 cut(s) 224
TspDTI ATGAA 1 cut(s) 210
TspGWI ACGGA 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.