AT4G35070

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0003008
Physical Location & Seq
Forward (+)
16694044 .. 16695711
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G35070.2

Sequence Viewer

Length: 798 bp
ATGGCTATACAAGCGCAGTTGAATTACAACGCTCCGAATGCGAATCAAATCGGTTTTGGTGGGTCCGAGTTTTCTTTGATCAACAACAATGGCGTTATCGGAATCGGTAACGATCAGTCTTATCTTGTCAATAATCTCCAGTTGCAGAAAGATTTCAACCAACATGCTCTGTTTCATCATCAGCATCATCAACAACAACAGTCTCCTTCTCAAAGCTTTTTAGCTGCTCAGATGGAGAAACAGAAGCAAGAGATCGATCAGTTCATCAAAATACAGAACGAGAGGTTGAGATATGTGTTGCAAGAACAGAGGAAGCGAGAAATGGAGATGATTTTACGGAAAATGGAGAGTAAAGCTTTGCTTTTGATGAGTCAGAAGGAAGAAGAAATGTCGAAAGCATTGAACAAGAACATGGAACTCGAAGATCTGTTGAGAAAAATGGAAATGGAGAATCAGACGTGGCAGAGAATGGCTCGTGAGAACGAAGCAATTGTGCAAACGCTTAACACAACGCTCGAACAGGTTCGCGAGAGAGCCGCCACGTGTTACGACGCTGGTGAGGCAGAGGTGGAGGACGAAGGGTCGTTTTGCGGCGGAGAAGGAGACGGGAATAGTTTGCCGGCGAAGAAGATGAAGATGAGTAGTTGTTGCTGCAATTGTGGGTCTAACGGAGTAACGAGAGTTCTGTTTCTGCCGTGTAGGCATCTCTGTTGCTGCATGGATTGCGAGGAAGGGCTTCTTCTTTGTCCGATCTGTAATACCCCCAAGAAAAGCAGAATCGAGGCCCTCATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

265

Amino Acids

30.25

Weight (kDa)

5.63

Isoelectric Point (pI)

56.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 160 - 203 9.7e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 528
AciI CCGC 3 cut(s) 537, 591, 594
AcvI CACGTG 1 cut(s) 543
AflIII ACRYGT 1 cut(s) 542
AgsI TTSAA 3 cut(s) 22, 157, 403
AhdI GACNNNNNGTC 1 cut(s) 580
AjiI CACGTC 1 cut(s) 459
AluBI AGCT 3 cut(s) 216, 224, 356
AluI AGCT 3 cut(s) 216, 224, 356
Alw26I GTCTC 2 cut(s) 207, 597
AoxI GGCC 1 cut(s) 783
ApeKI GCWGC 3 cut(s) 224, 651, 714
Asp700I GAANNNNTTC 3 cut(s) 152, 522, 735
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 2 cut(s) 63, 784
AsuHPI GGTGA 1 cut(s) 569
AvaII GGWCC 1 cut(s) 63
BauI CACGAG 1 cut(s) 474
BbrPI CACGTG 1 cut(s) 543
BbvI GCAGC 3 cut(s) 211, 638, 701
BccI CCATC 1 cut(s) 226
BceAI ACGGC 1 cut(s) 679
BclI TGATCA 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 207, 597
BfaI CTAG 1 cut(s) 796
BglI GCCNNNNNGGC 1 cut(s) 700
BglII AGATCT 1 cut(s) 424
BisI GCNGC 5 cut(s) 225, 537, 592, 652, 715
BlsI GCNGC 5 cut(s) 226, 538, 593, 653, 716
Bme18I GGWCC 1 cut(s) 63
BmeRI GACNNNNNGTC 1 cut(s) 580
BmgBI CACGTC 1 cut(s) 459
BmgT120I GGNCC 2 cut(s) 63, 784
BmiI GGNNCC 1 cut(s) 64
BmsI GCATC 2 cut(s) 193, 712
BplI GAGNNNNNCTC 2 cut(s) 457, 489
BpmI CTGGAG 1 cut(s) 122
Bsa29I ATCGAT 1 cut(s) 255
BsaAI YACGTR 1 cut(s) 543
Bse118I RCCGGY 1 cut(s) 619
Bse1I ACTGG 1 cut(s) 139
BseCI ATCGAT 1 cut(s) 255
BseMII CTCAG 1 cut(s) 242
BseNI ACTGG 1 cut(s) 139
BseXI GCAGC 3 cut(s) 211, 638, 701
Bsh1236I CGCG 1 cut(s) 528
BshFI GGCC 1 cut(s) 785
BshVI ATCGAT 1 cut(s) 255
BsiSI CCGG 1 cut(s) 620
BsmAI GTCTC 2 cut(s) 207, 597
BsmBI CGTCTC 1 cut(s) 597
BsmI GAATGC 1 cut(s) 43
BsnI GGCC 1 cut(s) 785
Bsp143I GATC 6 cut(s) 78, 112, 252, 256, 424, 750
Bsp68I TCGCGA 1 cut(s) 528
BspACI CCGC 3 cut(s) 537, 591, 594
BspANI GGCC 1 cut(s) 785
BspCNI CTCAG 1 cut(s) 241
BspDI ATCGAT 1 cut(s) 255
BspFNI CGCG 1 cut(s) 528
BspLI GGNNCC 1 cut(s) 64
BsrFI RCCGGY 1 cut(s) 619
BsrI ACTGG 1 cut(s) 139
BssAI RCCGGY 1 cut(s) 619
BssMI GATC 6 cut(s) 78, 112, 252, 256, 424, 750
BssSI CACGAG 1 cut(s) 474
Bst2BI CACGAG 1 cut(s) 474
Bst4CI ACNGT 1 cut(s) 201
BstAPI GCANNNNNTGC 1 cut(s) 723
BstBAI YACGTR 1 cut(s) 543
BstC8I GCNNGC 1 cut(s) 621
BstDEI CTNAG 1 cut(s) 228
BstFNI CGCG 1 cut(s) 528
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 6 cut(s) 81, 115, 255, 259, 427, 753
BstMAI GTCTC 2 cut(s) 207, 597
BstMBI GATC 6 cut(s) 78, 112, 252, 256, 424, 750
BstMWI GCNNNNNNNGC 5 cut(s) 11, 38, 560, 700, 723
BstNSI RCATGY 1 cut(s) 167
BstUI CGCG 1 cut(s) 528
BstV1I GCAGC 3 cut(s) 211, 638, 701
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
Bsu15I ATCGAT 1 cut(s) 255
BsuRI GGCC 1 cut(s) 785
BsuTUI ATCGAT 1 cut(s) 255
BtrI CACGTC 1 cut(s) 459
BtuMI TCGCGA 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 621
CfoI GCGC 1 cut(s) 16
Cfr10I RCCGGY 1 cut(s) 619
Cfr13I GGNCC 2 cut(s) 63, 784
ClaI ATCGAT 1 cut(s) 255
CseI GACGC 1 cut(s) 560
CviAII CATG 3 cut(s) 164, 412, 718
CviJI RGCY 8 cut(s) 5, 216, 224, 356, 473, 536, 736, 785
CviKI_1 RGCY 8 cut(s) 5, 216, 224, 356, 473, 536, 736, 785
DdeI CTNAG 1 cut(s) 228
DpnI GATC 6 cut(s) 80, 114, 254, 258, 426, 752
DpnII GATC 6 cut(s) 78, 112, 252, 256, 424, 750
DriI GACNNNNNGTC 1 cut(s) 580
Eam1105I GACNNNNNGTC 1 cut(s) 580
EciI GGCGGA 1 cut(s) 609
Eco47I GGWCC 1 cut(s) 63
Eco72I CACGTG 1 cut(s) 543
EcoO109I RGGNCCY 1 cut(s) 784
Esp3I CGTCTC 1 cut(s) 597
FaeI CATG 3 cut(s) 167, 415, 721
FaiI YATR 5 cut(s) 8, 165, 294, 413, 719
FalI AAGNNNNNCTT 4 cut(s) 345, 377, 723, 755
FatI CATG 3 cut(s) 163, 411, 717
FbaI TGATCA 1 cut(s) 78
Fnu4HI GCNGC 5 cut(s) 225, 537, 592, 652, 715
Fsp4HI GCNGC 5 cut(s) 225, 537, 592, 652, 715
FspBI CTAG 1 cut(s) 796
GlaI GCGC 1 cut(s) 15
GluI GCNGC 5 cut(s) 225, 537, 592, 652, 715
GsuI CTGGAG 1 cut(s) 122
HaeIII GGCC 1 cut(s) 785
HapII CCGG 1 cut(s) 620
HgaI GACGC 1 cut(s) 560
HhaI GCGC 1 cut(s) 16
Hin1II CATG 3 cut(s) 167, 415, 721
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HindIII AAGCTT 2 cut(s) 214, 354
HinfI GANTC 5 cut(s) 43, 102, 370, 451, 777
HpaII CCGG 1 cut(s) 620
HphI GGTGA 1 cut(s) 569
Hpy188I TCNGA 7 cut(s) 36, 67, 101, 231, 375, 456, 750
Hpy188III TCNNGA 2 cut(s) 476, 527
Hpy99I CGWCG 1 cut(s) 554
HpyAV CCTTC 5 cut(s) 216, 370, 572, 593, 725
HpyCH4III ACNGT 1 cut(s) 201
HpyCH4IV ACGT 2 cut(s) 458, 542
HpyCH4V TGCA 5 cut(s) 145, 301, 496, 654, 717
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 38, 560, 700, 723
HpyF3I CTNAG 1 cut(s) 228
HpySE526I ACGT 2 cut(s) 458, 542
Hsp92II CATG 3 cut(s) 167, 415, 721
HspAI GCGC 1 cut(s) 14
KroI GCCGGC 1 cut(s) 619
KroNI GCCGGC 1 cut(s) 621
Ksp22I TGATCA 1 cut(s) 78
Kzo9I GATC 6 cut(s) 78, 112, 252, 256, 424, 750
LmnI GCTCC 1 cut(s) 37
LpnPI CCDG 4 cut(s) 152, 506, 540, 633
Lsp1109I GCAGC 3 cut(s) 211, 638, 701
LweI GCATC 2 cut(s) 193, 712
MaeI CTAG 1 cut(s) 796
MaeII ACGT 2 cut(s) 458, 542
MaeIII GTNAC 3 cut(s) 107, 545, 673
MalI GATC 6 cut(s) 80, 114, 254, 258, 426, 752
MboI GATC 6 cut(s) 78, 112, 252, 256, 424, 750
MboII GAAGA 7 cut(s) 392, 395, 434, 637, 640, 646, 731
MfeI CAATTG 2 cut(s) 489, 655
MflI RGATCY 1 cut(s) 424
MluCI AATT 3 cut(s) 22, 489, 655
MlyI GAGTC 1 cut(s) 379
MnlI CCTC 8 cut(s) 276, 303, 553, 559, 565, 721, 775, 797
MroNI GCCGGC 1 cut(s) 619
MroXI GAANNNNTTC 3 cut(s) 152, 522, 735
MseI TTAA 1 cut(s) 504
MspI CCGG 1 cut(s) 620
MunI CAATTG 2 cut(s) 489, 655
Mva1269I GAATGC 1 cut(s) 43
MvnI CGCG 1 cut(s) 528
MwoI GCNNNNNNNGC 5 cut(s) 11, 38, 560, 700, 723
NaeI GCCGGC 1 cut(s) 621
NdeII GATC 6 cut(s) 78, 112, 252, 256, 424, 750
NgoMIV GCCGGC 1 cut(s) 619
NlaIII CATG 3 cut(s) 167, 415, 721
NlaIV GGNNCC 1 cut(s) 64
NruI TCGCGA 1 cut(s) 528
NspI RCATGY 1 cut(s) 167
PctI GAATGC 1 cut(s) 43
PdiI GCCGGC 1 cut(s) 621
PdmI GAANNNNTTC 3 cut(s) 152, 522, 735
PfeI GAWTC 4 cut(s) 43, 102, 451, 777
PkrI GCNGC 5 cut(s) 226, 538, 593, 653, 716
PleI GAGTC 1 cut(s) 378
PmaCI CACGTG 1 cut(s) 543
PmlI CACGTG 1 cut(s) 543
PpsI GAGTC 1 cut(s) 378
Ppu21I YACGTR 1 cut(s) 543
PspCI CACGTG 1 cut(s) 543
PspN4I GGNNCC 1 cut(s) 64
PspPI GGNCC 2 cut(s) 63, 784
PsrI GAACNNNNNNTAC 2 cut(s) 666, 698
PsuI RGATCY 1 cut(s) 424
RruI TCGCGA 1 cut(s) 528
SaqAI TTAA 1 cut(s) 504
SatI GCNGC 5 cut(s) 225, 537, 592, 652, 715
Sau3AI GATC 6 cut(s) 78, 112, 252, 256, 424, 750
Sau96I GGNCC 2 cut(s) 63, 784
SchI GAGTC 1 cut(s) 379
SetI ASST 8 cut(s) 218, 226, 287, 358, 461, 525, 545, 570
SfaNI GCATC 2 cut(s) 193, 712
SinI GGWCC 1 cut(s) 63
Sse9I AATT 3 cut(s) 22, 489, 655
SsiI CCGC 3 cut(s) 537, 591, 594
SspMI CTAG 1 cut(s) 796
TaaI ACNGT 1 cut(s) 201
TaiI ACGT 2 cut(s) 461, 545
TaqI TCGA 5 cut(s) 255, 392, 420, 516, 780
TasI AATT 3 cut(s) 22, 489, 655
TauI GCSGC 2 cut(s) 539, 594
TfiI GAWTC 4 cut(s) 43, 102, 451, 777
Tru1I TTAA 1 cut(s) 504
Tru9I TTAA 1 cut(s) 504
TseI GCWGC 3 cut(s) 224, 651, 714
TspDTI ATGAA 3 cut(s) 164, 253, 647
TspGWI ACGGA 2 cut(s) 352, 684
VpaK11BI GGWCC 1 cut(s) 63
XceI RCATGY 1 cut(s) 167
XmnI GAANNNNTTC 3 cut(s) 152, 522, 735
XspI CTAG 1 cut(s) 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.