MD15G1009900.v1.1

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0003008
Physical Location & Seq
Reverse (-)
535256 .. 536498
1243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1009900.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGCAGTTCAGGCACAAAGTTTGTACTTTTCTGAAGATTGGTCGTCGGCGGCAGCGGCGGCGCAGCTCCACCCTGTTTCGGGCCTTGACGACATTTTTTCCGGCATTCCGAGCCCTCAGCCCCCGGCTGTTCCTCCTCCTCATCATCACTTTCACCTCCGTCTTCCAGCGGCTCAGAATCAGAGTGAGAATTTAAATACGACGGGCTTTGATTTCTGCAACCAGTTAGGATCTTCGGTTTCGCCTTCCACTTTCAATAGCTTTCTCCCAACGCAGTTCTCGGAAAACTTAGGGGCGCAGATGGACTTGCAAAGGCACGAGCTTGACTGCATTATTCAATTGCAGAGTGAAAAGCTGAAGTTTGCATTGCAAGAGCAGAGGAAGCAACAGCTAGCAGCTCTATTGAGCAACCTAGAATCCAGAACGTTAAATTTAATTAGGCAGAAAGAAGACCAGTTAAGACAGGCAACCAAGAAAACGATGGAGCTCCAGGACTGCCTGAGAAAAGCGGAGATGGAAAGCGACACGTGGCAGCGGGTGGCGAAAGCGAACGAAACAATGGTGATGGGCCTAAACAACGCGCTCGTGGAGGCCAGAGAGCGACTGGTTCTGGTCAGCAGCGAAGCCGACGACGCAGAGTCCTGCTGTGAAAACAGAGTAGCAATTATGGAGGAAGATCAATTGGCCGAAGAGAAAAGGAGAAAGTTGGTTTGCAAAAGCTGCCACGCGCGGAGCTCGTGCGTGCTTTTTCTGCCGTGCAGGCATCTCTGTTCGTGCAAGTTCTGTGAAGGGTTTCTTGGATTCTGCCCCGTTTGTGATTCCACCAAGGAGGCAAGCATGGAGGTCTTTTTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

286

Amino Acids

31.97

Weight (kDa)

5.43

Isoelectric Point (pI)

62.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 236 - 276 2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 581, 728, 730
AciI CCGC 7 cut(s) 50, 56, 59, 170, 509, 535, 730
AclI AACGTT 1 cut(s) 425
AclWI GGATC 1 cut(s) 238
AcoI YGGCCR 1 cut(s) 684
AcsI RAATTY 2 cut(s) 190, 430
AcuI CTGAAG 2 cut(s) 54, 377
AcvI CACGTG 1 cut(s) 528
AfaI GTAC 1 cut(s) 26
AfiI CCNNNNNNNGG 2 cut(s) 79, 80
AflIII ACRYGT 1 cut(s) 525
AgsI TTSAA 2 cut(s) 256, 338
AhdI GACNNNNNGTC 1 cut(s) 637
AjnI CCWGG 1 cut(s) 489
AjuI GAANNNNNNNTTGG 2 cut(s) 780, 812
AluBI AGCT 9 cut(s) 67, 261, 322, 355, 391, 398, 487, 720, 735
AluI AGCT 9 cut(s) 67, 261, 322, 355, 391, 398, 487, 720, 735
Alw21I GWGCWC 2 cut(s) 489, 737
AlwI GGATC 1 cut(s) 238
AoxI GGCC 4 cut(s) 82, 568, 591, 684
ApeKI GCWGC 6 cut(s) 53, 64, 395, 532, 618, 720
ApoI RAATTY 2 cut(s) 190, 430
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 4 cut(s) 64, 298, 583, 730
AspS9I GGNCC 2 cut(s) 82, 568
AsuC2I CCSGG 1 cut(s) 125
AsuHPI GGTGA 2 cut(s) 146, 574
AsuNHI GCTAGC 1 cut(s) 391
BanII GRGCYC 3 cut(s) 116, 489, 737
BauI CACGAG 3 cut(s) 317, 584, 736
BbrPI CACGTG 1 cut(s) 528
BbsI GAAGAC 2 cut(s) 155, 456
Bbv12I GWGCWC 2 cut(s) 489, 737
BbvCI CCTCAGC 1 cut(s) 117
BbvI GCAGC 6 cut(s) 65, 76, 407, 544, 630, 707
BccI CCATC 4 cut(s) 295, 475, 508, 559
BceAI ACGGC 1 cut(s) 739
BciT130I CCWGG 1 cut(s) 491
BcnI CCSGG 1 cut(s) 125
BfaI CTAG 3 cut(s) 392, 413, 856
BglI GCCNNNNNGGC 1 cut(s) 760
Bme1390I CCNGG 2 cut(s) 125, 491
BmeRI GACNNNNNGTC 1 cut(s) 637
BmgT120I GGNCC 2 cut(s) 82, 568
BmrFI CCNGG 2 cut(s) 125, 491
BmsI GCATC 1 cut(s) 772
BmtI GCTAGC 1 cut(s) 395
BpiI GAAGAC 2 cut(s) 155, 456
BpmI CTGGAG 1 cut(s) 473
Bpu10I CCTNAGC 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 528
BsaJI CCNNGG 2 cut(s) 123, 825
Bsc4I CCNNNNNNNGG 2 cut(s) 79, 80
Bse1I ACTGG 3 cut(s) 223, 454, 609
Bse3DI GCAATG 1 cut(s) 365
BseBI CCWGG 1 cut(s) 491
BseDI CCNNGG 2 cut(s) 123, 825
BseLI CCNNNNNNNGG 2 cut(s) 79, 80
BseMI GCAATG 1 cut(s) 365
BseMII CTCAG 3 cut(s) 131, 188, 491
BseNI ACTGG 3 cut(s) 223, 454, 609
BseRI GAGGAG 2 cut(s) 126, 129
BseXI GCAGC 6 cut(s) 65, 76, 407, 544, 630, 707
BsgI GTGCAG 1 cut(s) 778
Bsh1236I CGCG 3 cut(s) 581, 728, 730
BshFI GGCC 4 cut(s) 84, 570, 593, 686
BsiHKAI GWGCWC 2 cut(s) 489, 737
BsiSI CCGG 2 cut(s) 102, 125
BslI CCNNNNNNNGG 2 cut(s) 79, 80
BsmI GAATGC 1 cut(s) 105
BsnI GGCC 4 cut(s) 84, 570, 593, 686
Bsp1286I GDGCHC 3 cut(s) 116, 489, 737
Bsp143I GATC 2 cut(s) 230, 676
BspACI CCGC 7 cut(s) 50, 56, 59, 170, 509, 535, 730
BspANI GGCC 4 cut(s) 84, 570, 593, 686
BspCNI CTCAG 3 cut(s) 130, 187, 492
BspFNI CGCG 3 cut(s) 581, 728, 730
BspOI GCTAGC 1 cut(s) 395
BspPI GGATC 1 cut(s) 238
BsrDI GCAATG 1 cut(s) 365
BsrI ACTGG 3 cut(s) 223, 454, 609
BssECI CCNNGG 2 cut(s) 123, 825
BssMI GATC 2 cut(s) 230, 676
BssSI CACGAG 3 cut(s) 317, 584, 736
BssT1I CCWWGG 1 cut(s) 825
Bst2BI CACGAG 3 cut(s) 317, 584, 736
Bst2UI CCWGG 1 cut(s) 491
Bst6I CTCTTC 1 cut(s) 684
BstAPI GCANNNNNTGC 1 cut(s) 720
BstBAI YACGTR 1 cut(s) 528
BstC8I GCNNGC 4 cut(s) 393, 743, 761, 835
BstDEI CTNAG 4 cut(s) 117, 174, 289, 500
BstFNI CGCG 3 cut(s) 581, 728, 730
BstHHI GCGC 4 cut(s) 64, 298, 583, 730
BstKTI GATC 2 cut(s) 233, 679
BstMBI GATC 2 cut(s) 230, 676
BstMWI GCNNNNNNNGC 9 cut(s) 11, 56, 59, 111, 382, 632, 720, 751, 760
BstNI CCWGG 1 cut(s) 491
BstSCI CCNGG 2 cut(s) 123, 489
BstUI CGCG 3 cut(s) 581, 728, 730
BstV1I GCAGC 6 cut(s) 65, 76, 407, 544, 630, 707
BstV2I GAAGAC 2 cut(s) 155, 456
BstX2I RGATCY 1 cut(s) 230
BstYI RGATCY 1 cut(s) 230
BsuRI GGCC 4 cut(s) 84, 570, 593, 686
Cac8I GCNNGC 4 cut(s) 393, 743, 761, 835
CfoI GCGC 4 cut(s) 64, 298, 583, 730
Cfr13I GGNCC 2 cut(s) 82, 568
CseI GACGC 1 cut(s) 641
Csp6I GTAC 1 cut(s) 25
CviAII CATG 1 cut(s) 838
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 4 cut(s) 117, 174, 289, 500
DpnI GATC 2 cut(s) 232, 678
DpnII GATC 2 cut(s) 230, 676
DraI TTTAAA 1 cut(s) 195
DriI GACNNNNNGTC 1 cut(s) 637
EaeI YGGCCR 1 cut(s) 684
Eam1104I CTCTTC 1 cut(s) 684
Eam1105I GACNNNNNGTC 1 cut(s) 637
EarI CTCTTC 1 cut(s) 684
Ecl136II GAGCTC 2 cut(s) 487, 735
Eco130I CCWWGG 1 cut(s) 825
Eco24I GRGCYC 3 cut(s) 116, 489, 737
Eco53kI GAGCTC 2 cut(s) 487, 735
Eco57I CTGAAG 2 cut(s) 54, 377
Eco72I CACGTG 1 cut(s) 528
EcoICRI GAGCTC 2 cut(s) 487, 735
EcoRII CCWGG 1 cut(s) 489
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 3 cut(s) 116, 489, 737
ErhI CCWWGG 1 cut(s) 825
FaeI CATG 1 cut(s) 841
FaiI YATR 2 cut(s) 668, 839
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FatI CATG 1 cut(s) 837
FauI CCCGC 1 cut(s) 528
FriOI GRGCYC 3 cut(s) 116, 489, 737
FspBI CTAG 3 cut(s) 392, 413, 856
GlaI GCGC 4 cut(s) 63, 297, 582, 729
GsuI CTGGAG 1 cut(s) 473
HaeIII GGCC 4 cut(s) 84, 570, 593, 686
HapII CCGG 2 cut(s) 102, 125
HgaI GACGC 1 cut(s) 641
HhaI GCGC 4 cut(s) 64, 298, 583, 730
Hin1II CATG 1 cut(s) 841
Hin6I GCGC 4 cut(s) 62, 296, 581, 728
HinP1I GCGC 4 cut(s) 62, 296, 581, 728
HinfI GANTC 5 cut(s) 178, 416, 638, 801, 818
HpaII CCGG 2 cut(s) 102, 125
HphI GGTGA 2 cut(s) 146, 574
Hpy188I TCNGA 5 cut(s) 34, 111, 177, 183, 283
Hpy188III TCNNGA 1 cut(s) 420
Hpy99I CGWCG 4 cut(s) 49, 205, 632, 635
HpyAV CCTTC 2 cut(s) 255, 782
HpyCH4IV ACGT 2 cut(s) 425, 527
HpyCH4V TGCA 9 cut(s) 219, 310, 330, 343, 365, 370, 714, 759, 777
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 56, 59, 111, 382, 632, 720, 751, 760
HpyF3I CTNAG 4 cut(s) 117, 174, 289, 500
HpySE526I ACGT 2 cut(s) 425, 527
Hsp92II CATG 1 cut(s) 841
HspAI GCGC 4 cut(s) 62, 296, 581, 728
Kzo9I GATC 2 cut(s) 230, 676
LmnI GCTCC 4 cut(s) 72, 484, 492, 732
Lsp1109I GCAGC 6 cut(s) 65, 76, 407, 544, 630, 707
LweI GCATC 1 cut(s) 772
MaeI CTAG 3 cut(s) 392, 413, 856
MaeII ACGT 2 cut(s) 425, 527
MalI GATC 2 cut(s) 232, 678
MboI GATC 2 cut(s) 230, 676
MboII GAAGA 6 cut(s) 47, 155, 225, 461, 686, 701
MfeI CAATTG 2 cut(s) 338, 680
MflI RGATCY 1 cut(s) 230
MhlI GDGCHC 3 cut(s) 116, 489, 737
MluCI AATT 6 cut(s) 190, 338, 430, 435, 663, 680
MlyI GAGTC 1 cut(s) 647
MroXI GAANNNNTTC 1 cut(s) 792
MseI TTAA 4 cut(s) 194, 428, 434, 458
MspA1I CMGCKG 3 cut(s) 56, 170, 535
MspI CCGG 2 cut(s) 102, 125
MspR9I CCNGG 2 cut(s) 125, 491
MunI CAATTG 2 cut(s) 338, 680
Mva1269I GAATGC 1 cut(s) 105
MvaI CCWGG 1 cut(s) 491
MvnI CGCG 3 cut(s) 581, 728, 730
MwoI GCNNNNNNNGC 9 cut(s) 11, 56, 59, 111, 382, 632, 720, 751, 760
NciI CCSGG 1 cut(s) 125
NdeII GATC 2 cut(s) 230, 676
NheI GCTAGC 1 cut(s) 391
NlaIII CATG 1 cut(s) 841
PctI GAATGC 1 cut(s) 105
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 4 cut(s) 178, 416, 801, 818
PfoI TCCNGGA 1 cut(s) 489
PleI GAGTC 1 cut(s) 646
PmaCI CACGTG 1 cut(s) 528
PmlI CACGTG 1 cut(s) 528
PpsI GAGTC 1 cut(s) 646
Ppu21I YACGTR 1 cut(s) 528
Psp124BI GAGCTC 2 cut(s) 489, 737
Psp1406I AACGTT 1 cut(s) 425
Psp6I CCWGG 1 cut(s) 489
PspCI CACGTG 1 cut(s) 528
PspGI CCWGG 1 cut(s) 489
PspPI GGNCC 2 cut(s) 82, 568
PsuI RGATCY 1 cut(s) 230
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SacI GAGCTC 2 cut(s) 489, 737
SaqAI TTAA 4 cut(s) 194, 428, 434, 458
Sau3AI GATC 2 cut(s) 230, 676
Sau96I GGNCC 2 cut(s) 82, 568
SchI GAGTC 1 cut(s) 647
ScrFI CCNGG 2 cut(s) 125, 491
SduI GDGCHC 3 cut(s) 116, 489, 737
SfaNI GCATC 1 cut(s) 772
SmiI ATTTAAAT 1 cut(s) 195
Sse9I AATT 6 cut(s) 190, 338, 430, 435, 663, 680
SsiI CCGC 7 cut(s) 50, 56, 59, 170, 509, 535, 730
SspMI CTAG 3 cut(s) 392, 413, 856
SstI GAGCTC 2 cut(s) 489, 737
StyD4I CCNGG 2 cut(s) 123, 489
StyI CCWWGG 1 cut(s) 825
SwaI ATTTAAAT 1 cut(s) 195
TaiI ACGT 2 cut(s) 428, 530
TasI AATT 6 cut(s) 190, 338, 430, 435, 663, 680
TatI WGTACW 1 cut(s) 24
TauI GCSGC 4 cut(s) 53, 59, 62, 173
TfiI GAWTC 4 cut(s) 178, 416, 801, 818
Tru1I TTAA 4 cut(s) 194, 428, 434, 458
Tru9I TTAA 4 cut(s) 194, 428, 434, 458
TseI GCWGC 6 cut(s) 53, 64, 395, 532, 618, 720
TspGWI ACGGA 1 cut(s) 149
XapI RAATTY 2 cut(s) 190, 430
XcmI CCANNNNNNNNNTGG 2 cut(s) 478, 601
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 3 cut(s) 392, 413, 856
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.