MD08G1010000.v1.1

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003008
Physical Location & Seq
Reverse (-)
747857 .. 749731
1875 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1010000.v1.1.491

Sequence Viewer

Length: 837 bp
ATGGCAGTTCAGGCACAAAGTTTGTATTTTTCTGAAGATTGGTCGTCGGCGGCGGCGCTGCTCCACCCTGTTTCCGGCCTCGACGACATATTTTCCGGCATTCCGAGCCCTCCTCCTCCTCAAAATCACTTTCACCTCCGTCTTCCGGCGGCACAGAATCAGAGTCAGAATTTAAACACGACGGGCTTTGATTTCTGCAACCCATTAGGAGCTTCGGTTTCGCCTTCCACTTTCAGTAGCTTTCTCCCAACGCAGTTCTCGGAAAACTTAGGGGCTCTGCTGGACTTGCAAAGGCACGAGCTCGACTGCATTATTCAATTCCAGAGTGAAAAGCTGAAGTTCGAATTGCAAGAGCAGAGGAAGCAGCAACTAGCAGCTTTGTTGAGCAACTTAGAATCCACAACGTTAAATTTAATTAGGCAGAAAGAAGAAGAGTTAGGACAGGCGACAAAGAAAGCGATGGAGCTCCAGGACTGCCTGAGAAAAGCGGAGATGGAAAGCGATACGTGGCAGCGGATGGCGAAGGCGAACGAATCAATGGTGATGGACCTGAACAACGCGCTCGAGCAGGTCAGAGAACGACTGGTTCTCGTCAGCAGCGAAACCGACGACGCAGAGTCCTGCTGTGAAAACAGAGTAGCAATTACGGAGGAAGATCAATTGGCGGAAGAGAAAAGGAGAAAGTTGGTATGCAAAAGCTGCCGCGCGCGGAGCTCGTGCGTGCTTTTTCTGCCGTGCAGGCATCTCTGTTCGTGCAAGTTCTGTGAAGGGTTTCTTGGATTTTGCCCCGTTTGTGGTTCCGCCAAGGAGGCAAGCATGGAGGTCTTTTTTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

279

Amino Acids

31.17

Weight (kDa)

5.04

Isoelectric Point (pI)

59.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 229 - 269 1.4e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 559
AccII CGCG 4 cut(s) 560, 705, 707, 709
AciI CCGC 9 cut(s) 50, 53, 149, 488, 514, 665, 703, 709, 801
AclI AACGTT 1 cut(s) 404
AcsI RAATTY 2 cut(s) 169, 409
AcuI CTGAAG 2 cut(s) 54, 356
AfiI CCNNNNNNNGG 3 cut(s) 74, 145, 794
AgsI TTSAA 1 cut(s) 317
AhdI GACNNNNNGTC 1 cut(s) 616
AjnI CCWGG 1 cut(s) 468
AjuI GAANNNNNNNTTGG 2 cut(s) 759, 791
AluBI AGCT 8 cut(s) 212, 240, 301, 334, 377, 466, 699, 714
AluI AGCT 8 cut(s) 212, 240, 301, 334, 377, 466, 699, 714
Alw21I GWGCWC 3 cut(s) 303, 468, 716
Ama87I CYCGRG 1 cut(s) 563
AoxI GGCC 1 cut(s) 76
ApeKI GCWGC 6 cut(s) 58, 364, 374, 511, 597, 699
ApoI RAATTY 2 cut(s) 169, 409
Asp700I GAANNNNTTC 1 cut(s) 771
AspLEI GCGC 4 cut(s) 58, 562, 707, 709
AspS9I GGNCC 1 cut(s) 547
AsuHPI GGTGA 2 cut(s) 125, 553
AsuII TTCGAA 1 cut(s) 342
AvaI CYCGRG 1 cut(s) 563
AvaII GGWCC 1 cut(s) 547
BanII GRGCYC 5 cut(s) 110, 277, 303, 468, 716
BauI CACGAG 2 cut(s) 296, 715
BbsI GAAGAC 1 cut(s) 134
Bbv12I GWGCWC 3 cut(s) 303, 468, 716
BbvI GCAGC 6 cut(s) 45, 376, 386, 523, 609, 686
BccI CCATC 4 cut(s) 454, 487, 511, 538
BceAI ACGGC 1 cut(s) 718
BciT130I CCWGG 1 cut(s) 470
BfaI CTAG 2 cut(s) 371, 835
BfoI RGCGCY 1 cut(s) 59
BfuAI ACCTGC 1 cut(s) 559
BglI GCCNNNNNGGC 2 cut(s) 739, 809
Bme1390I CCNGG 1 cut(s) 470
Bme18I GGWCC 1 cut(s) 547
BmeRI GACNNNNNGTC 1 cut(s) 616
BmeT110I CYCGRG 1 cut(s) 563
BmgT120I GGNCC 1 cut(s) 547
BmiI GGNNCC 1 cut(s) 799
BmrFI CCNGG 1 cut(s) 470
BmsI GCATC 1 cut(s) 751
BpiI GAAGAC 1 cut(s) 134
BplI GAGNNNNNCTC 2 cut(s) 97, 129
BpmI CTGGAG 1 cut(s) 452
Bpu14I TTCGAA 1 cut(s) 342
BsaAI YACGTR 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 804
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 145, 794
Bse1I ACTGG 1 cut(s) 588
BseBI CCWGG 1 cut(s) 470
BseDI CCNNGG 1 cut(s) 804
BseGI GGATG 1 cut(s) 522
BseLI CCNNNNNNNGG 3 cut(s) 74, 145, 794
BseMII CTCAG 1 cut(s) 470
BseNI ACTGG 1 cut(s) 588
BsePI GCGCGC 1 cut(s) 705
BseRI GAGGAG 3 cut(s) 102, 105, 108
BseXI GCAGC 6 cut(s) 45, 376, 386, 523, 609, 686
BsgI GTGCAG 1 cut(s) 757
Bsh1236I CGCG 4 cut(s) 560, 705, 707, 709
BshFI GGCC 1 cut(s) 78
BsiHKAI GWGCWC 3 cut(s) 303, 468, 716
BsiHKCI CYCGRG 1 cut(s) 563
BsiSI CCGG 3 cut(s) 75, 96, 146
BslI CCNNNNNNNGG 3 cut(s) 74, 145, 794
BsmI GAATGC 1 cut(s) 99
BsnI GGCC 1 cut(s) 78
BsoBI CYCGRG 1 cut(s) 563
Bsp119I TTCGAA 1 cut(s) 342
Bsp1286I GDGCHC 5 cut(s) 110, 277, 303, 468, 716
Bsp143I GATC 1 cut(s) 655
BspACI CCGC 9 cut(s) 50, 53, 149, 488, 514, 665, 703, 709, 801
BspANI GGCC 1 cut(s) 78
BspCNI CTCAG 1 cut(s) 471
BspFNI CGCG 4 cut(s) 560, 705, 707, 709
BspLI GGNNCC 1 cut(s) 799
BspMI ACCTGC 1 cut(s) 559
BspT104I TTCGAA 1 cut(s) 342
BsrI ACTGG 1 cut(s) 588
BssECI CCNNGG 1 cut(s) 804
BssHII GCGCGC 1 cut(s) 705
BssMI GATC 1 cut(s) 655
BssSI CACGAG 2 cut(s) 296, 715
BssT1I CCWWGG 1 cut(s) 804
Bst2BI CACGAG 2 cut(s) 296, 715
Bst2UI CCWGG 1 cut(s) 470
Bst6I CTCTTC 2 cut(s) 426, 663
BstAPI GCANNNNNTGC 1 cut(s) 699
BstBAI YACGTR 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 342
BstC8I GCNNGC 4 cut(s) 707, 722, 740, 814
BstDEI CTNAG 3 cut(s) 268, 391, 479
BstF5I GGATG 1 cut(s) 522
BstFNI CGCG 4 cut(s) 560, 705, 707, 709
BstH2I RGCGCY 1 cut(s) 59
BstHHI GCGC 4 cut(s) 58, 562, 707, 709
BstKTI GATC 1 cut(s) 658
BstMBI GATC 1 cut(s) 655
BstMWI GCNNNNNNNGC 9 cut(s) 11, 105, 286, 361, 699, 711, 730, 739, 809
BstNI CCWGG 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 468
BstUI CGCG 4 cut(s) 560, 705, 707, 709
BstV1I GCAGC 6 cut(s) 45, 376, 386, 523, 609, 686
BstV2I GAAGAC 1 cut(s) 134
BsuRI GGCC 1 cut(s) 78
BtgZI GCGATG 1 cut(s) 473
BtsCI GGATG 1 cut(s) 522
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 4 cut(s) 707, 722, 740, 814
CfoI GCGC 4 cut(s) 58, 562, 707, 709
Cfr13I GGNCC 1 cut(s) 547
CseI GACGC 1 cut(s) 620
CviAII CATG 1 cut(s) 817
DdeI CTNAG 3 cut(s) 268, 391, 479
DpnI GATC 1 cut(s) 657
DpnII GATC 1 cut(s) 655
DraI TTTAAA 1 cut(s) 174
DriI GACNNNNNGTC 1 cut(s) 616
Eam1104I CTCTTC 2 cut(s) 426, 663
Eam1105I GACNNNNNGTC 1 cut(s) 616
EarI CTCTTC 2 cut(s) 426, 663
EciI GGCGGA 2 cut(s) 680, 790
Ecl136II GAGCTC 3 cut(s) 301, 466, 714
Eco130I CCWWGG 1 cut(s) 804
Eco24I GRGCYC 5 cut(s) 110, 277, 303, 468, 716
Eco47I GGWCC 1 cut(s) 547
Eco53kI GAGCTC 3 cut(s) 301, 466, 714
Eco57I CTGAAG 2 cut(s) 54, 356
Eco88I CYCGRG 1 cut(s) 563
EcoICRI GAGCTC 3 cut(s) 301, 466, 714
EcoRII CCWGG 1 cut(s) 468
EcoT14I CCWWGG 1 cut(s) 804
EcoT38I GRGCYC 5 cut(s) 110, 277, 303, 468, 716
ErhI CCWWGG 1 cut(s) 804
FaeI CATG 1 cut(s) 820
FaiI YATR 3 cut(s) 89, 691, 818
FalI AAGNNNNNCTT 2 cut(s) 759, 791
FatI CATG 1 cut(s) 816
FokI GGATG 1 cut(s) 529
FriOI GRGCYC 5 cut(s) 110, 277, 303, 468, 716
FspBI CTAG 2 cut(s) 371, 835
GlaI GCGC 4 cut(s) 57, 561, 706, 708
GsuI CTGGAG 1 cut(s) 452
HaeII RGCGCY 1 cut(s) 59
HaeIII GGCC 1 cut(s) 78
HapII CCGG 3 cut(s) 75, 96, 146
HgaI GACGC 1 cut(s) 620
HhaI GCGC 4 cut(s) 58, 562, 707, 709
Hin1II CATG 1 cut(s) 820
Hin6I GCGC 4 cut(s) 56, 560, 705, 707
HinP1I GCGC 4 cut(s) 56, 560, 705, 707
HinfI GANTC 5 cut(s) 157, 163, 395, 533, 617
HpaII CCGG 3 cut(s) 75, 96, 146
HphI GGTGA 2 cut(s) 125, 553
Hpy188I TCNGA 6 cut(s) 34, 105, 162, 168, 262, 575
Hpy188III TCNNGA 1 cut(s) 322
Hpy99I CGWCG 5 cut(s) 49, 86, 184, 611, 614
HpyAV CCTTC 3 cut(s) 234, 517, 761
HpyCH4IV ACGT 2 cut(s) 404, 506
HpyCH4V TGCA 7 cut(s) 198, 289, 309, 349, 693, 738, 756
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 105, 286, 361, 699, 711, 730, 739, 809
HpyF3I CTNAG 3 cut(s) 268, 391, 479
HpySE526I ACGT 2 cut(s) 404, 506
Hsp92II CATG 1 cut(s) 820
HspAI GCGC 4 cut(s) 56, 560, 705, 707
Kzo9I GATC 1 cut(s) 655
LmnI GCTCC 5 cut(s) 66, 209, 463, 471, 711
Lsp1109I GCAGC 6 cut(s) 45, 376, 386, 523, 609, 686
LweI GCATC 1 cut(s) 751
MaeI CTAG 2 cut(s) 371, 835
MaeII ACGT 2 cut(s) 404, 506
MalI GATC 1 cut(s) 657
MauBI CGCGCGCG 1 cut(s) 705
MboI GATC 1 cut(s) 655
MboII GAAGA 6 cut(s) 47, 134, 440, 443, 665, 680
MfeI CAATTG 1 cut(s) 659
MhlI GDGCHC 5 cut(s) 110, 277, 303, 468, 716
MluCI AATT 7 cut(s) 169, 317, 344, 409, 414, 642, 659
MlyI GAGTC 2 cut(s) 172, 626
MroXI GAANNNNTTC 1 cut(s) 771
MseI TTAA 3 cut(s) 173, 407, 413
MspA1I CMGCKG 1 cut(s) 514
MspI CCGG 3 cut(s) 75, 96, 146
MspR9I CCNGG 1 cut(s) 470
MunI CAATTG 1 cut(s) 659
Mva1269I GAATGC 1 cut(s) 99
MvaI CCWGG 1 cut(s) 470
MvnI CGCG 4 cut(s) 560, 705, 707, 709
MwoI GCNNNNNNNGC 9 cut(s) 11, 105, 286, 361, 699, 711, 730, 739, 809
NdeII GATC 1 cut(s) 655
NlaIII CATG 1 cut(s) 820
NlaIV GGNNCC 1 cut(s) 799
NspV TTCGAA 1 cut(s) 342
PaeR7I CTCGAG 1 cut(s) 563
PauI GCGCGC 1 cut(s) 705
PcsI WCGNNNNNNNCGW 1 cut(s) 597
PctI GAATGC 1 cut(s) 99
PdmI GAANNNNTTC 1 cut(s) 771
PfeI GAWTC 3 cut(s) 157, 395, 533
PfoI TCCNGGA 1 cut(s) 468
PleI GAGTC 2 cut(s) 171, 625
PpsI GAGTC 2 cut(s) 171, 625
Ppu21I YACGTR 1 cut(s) 507
Psp124BI GAGCTC 3 cut(s) 303, 468, 716
Psp1406I AACGTT 1 cut(s) 404
Psp6I CCWGG 1 cut(s) 468
PspGI CCWGG 1 cut(s) 468
PspN4I GGNNCC 1 cut(s) 799
PspPI GGNCC 1 cut(s) 547
PspXI VCTCGAGB 1 cut(s) 563
PteI GCGCGC 1 cut(s) 705
SacI GAGCTC 3 cut(s) 303, 468, 716
SaqAI TTAA 3 cut(s) 173, 407, 413
Sau3AI GATC 1 cut(s) 655
Sau96I GGNCC 1 cut(s) 547
SchI GAGTC 2 cut(s) 172, 626
ScrFI CCNGG 1 cut(s) 470
SduI GDGCHC 5 cut(s) 110, 277, 303, 468, 716
SfaNI GCATC 1 cut(s) 751
Sfr274I CTCGAG 1 cut(s) 563
SfuI TTCGAA 1 cut(s) 342
SinI GGWCC 1 cut(s) 547
SlaI CTCGAG 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 563
SmoI CTYRAG 1 cut(s) 563
Sse9I AATT 7 cut(s) 169, 317, 344, 409, 414, 642, 659
SsiI CCGC 9 cut(s) 50, 53, 149, 488, 514, 665, 703, 709, 801
SspMI CTAG 2 cut(s) 371, 835
SstI GAGCTC 3 cut(s) 303, 468, 716
StyD4I CCNGG 1 cut(s) 468
StyI CCWWGG 1 cut(s) 804
TaiI ACGT 2 cut(s) 407, 509
TaqI TCGA 4 cut(s) 81, 303, 342, 564
TasI AATT 7 cut(s) 169, 317, 344, 409, 414, 642, 659
TauI GCSGC 4 cut(s) 53, 56, 152, 705
TfiI GAWTC 3 cut(s) 157, 395, 533
Tru1I TTAA 3 cut(s) 173, 407, 413
Tru9I TTAA 3 cut(s) 173, 407, 413
TseI GCWGC 6 cut(s) 58, 364, 374, 511, 597, 699
TspGWI ACGGA 2 cut(s) 128, 662
VpaK11BI GGWCC 1 cut(s) 547
XapI RAATTY 2 cut(s) 169, 409
XhoI CTCGAG 1 cut(s) 563
XmnI GAANNNNTTC 1 cut(s) 771
XspI CTAG 2 cut(s) 371, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.