RLG00000011090

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003008
Physical Location & Seq
Reverse (-)
6731797 .. 6732734
938 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011090

Sequence Viewer

Length: 858 bp
ATGGCAGTTCAAGCTCAGACCATGTATTTCTCAGAAAATTTGGGCATGAGTGGCTTTCCACAGCAACAGGAATGGGCTCTGATCAACCCTCCTCCGCTTTCCGATTCCGGGTTTGATCATGACTTCTTTTCCGGCATCATTCCAAACCCTCAACCTCATCAGCAGCAACAAGCTCCTCAGTTTTATCAAAATCTAGAAGCTAACCGGTTAGGAGTTTCTTCTGTCTCTCCTAATTCCACTTGCAATAGCTTCCTTCCCACAACATCATTATTTCCCGATGCTCTGACCGCTCAGCTTGACTTGCAAAGGCGGGAGTTAGATTGCATTCTTCAGTTGCAGAATGAGAAGCTGAGATTTGCTTTGCAAGAGCAGAGGAAGCGACAGCTGGCGGCTCTGTTGAGCAACTTCGAGTCGAGGACGTCGAGTTTGATGAGGCAAAAAGAGGAGCACTTATCACAAGCGAGAAAGAGAGCAATGGAGCTTCAAGATTGCTTGAGAAAAGCGGAGATGGAGACCGAGACGTGGCAGAGATTGGCCAAAGCAAACGAGACGACGGTGATGGACCTCAACAACGCGCTCGAACAGGTCAAAGAGAGAATGATCCTGTGCAGCAACGAAGCCGATGATGCAGAGTCCTTCTGTGGCTCGTGTGAGAAGCAGCAGCAACAAGTGCTTTCAGGAAAAGCAGCTCACAAAAAGCTGGGTTTGGATTGTAAAAGCTGTAATGCTAGAAGGTCGTGCGTGCTTTTTCTTCCTTGCAGACACCTCTGTTCGTGCAAGTTTTGTGAAGCTCTTCTTGATTGCTGCCCCGTGTGTAAATCCACCAAGGAGGCAAGCATGGAGGTCTTTTTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

286

Amino Acids

32.28

Weight (kDa)

6.02

Isoelectric Point (pI)

59.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 237 - 277 2.4e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 290
AccII CGCG 1 cut(s) 575
AciI CCGC 5 cut(s) 95, 288, 310, 389, 503
AclWI GGATC 1 cut(s) 595
AcoI YGGCCR 1 cut(s) 534
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 314
AcyI GRCGYC 1 cut(s) 419
AfiI CCNNNNNNNGG 2 cut(s) 108, 522
AgeI ACCGGT 1 cut(s) 204
AgsI TTSAA 2 cut(s) 11, 485
AjiI CACGTC 1 cut(s) 522
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 4 cut(s) 229, 506, 512, 542
AlwI GGATC 1 cut(s) 595
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 6 cut(s) 163, 609, 658, 661, 686, 804
ApoI RAATTY 1 cut(s) 37
ArsI GACNNNNNNTTYG 2 cut(s) 409, 441
AsiGI ACCGGT 1 cut(s) 204
Asp700I GAANNNNTTC 1 cut(s) 792
AspLEI GCGC 1 cut(s) 577
AspS9I GGNCC 1 cut(s) 562
AsuC2I CCSGG 1 cut(s) 109
AsuHPI GGTGA 1 cut(s) 568
AvaII GGWCC 1 cut(s) 562
BalI TGGCCA 1 cut(s) 536
BanII GRGCYC 1 cut(s) 79
BauI CACGAG 1 cut(s) 646
Bbv12I GWGCWC 1 cut(s) 450
BbvI GCAGC 6 cut(s) 175, 621, 670, 673, 698, 791
BccI CCATC 2 cut(s) 502, 553
BclI TGATCA 2 cut(s) 81, 115
BcnI CCSGG 1 cut(s) 109
BcoDI GTCTC 4 cut(s) 229, 506, 512, 542
BfaI CTAG 2 cut(s) 194, 729
BisI GCNGC 7 cut(s) 164, 390, 610, 659, 662, 687, 805
BlpI GCTNAGC 1 cut(s) 291
BlsI GCNGC 7 cut(s) 165, 391, 611, 660, 663, 688, 806
Bme1390I CCNGG 1 cut(s) 109
Bme18I GGWCC 1 cut(s) 562
BmgBI CACGTC 1 cut(s) 522
BmgT120I GGNCC 1 cut(s) 562
BmrFI CCNGG 1 cut(s) 109
BmsI GCATC 3 cut(s) 144, 268, 616
Bpu1102I GCTNAGC 1 cut(s) 291
BpuEI CTTGAG 1 cut(s) 514
BpuMI CCSGG 1 cut(s) 109
BsaHI GRCGYC 1 cut(s) 419
BsaI GGTCTC 1 cut(s) 506
BsaJI CCNNGG 1 cut(s) 825
BsaWI WCCGGW 1 cut(s) 204
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 522
Bse118I RCCGGY 1 cut(s) 204
Bse3DI GCAATG 1 cut(s) 480
BseDI CCNNGG 1 cut(s) 825
BseLI CCNNNNNNNGG 2 cut(s) 108, 522
BseMI GCAATG 1 cut(s) 480
BseMII CTCAG 5 cut(s) 29, 45, 191, 305, 341
BseRI GAGGAG 3 cut(s) 81, 165, 458
BseXI GCAGC 6 cut(s) 175, 621, 670, 673, 698, 791
BseYI CCCAGC 1 cut(s) 700
BsgI GTGCAG 1 cut(s) 628
Bsh1236I CGCG 1 cut(s) 575
BshFI GGCC 1 cut(s) 536
BshTI ACCGGT 1 cut(s) 204
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 3 cut(s) 108, 132, 205
BslI CCNNNNNNNGG 2 cut(s) 108, 522
BsmAI GTCTC 4 cut(s) 229, 506, 512, 542
BsmBI CGTCTC 2 cut(s) 512, 542
BsmI GAATGC 1 cut(s) 324
BsnI GGCC 1 cut(s) 536
Bso31I GGTCTC 1 cut(s) 506
Bsp1286I GDGCHC 2 cut(s) 79, 450
Bsp143I GATC 3 cut(s) 81, 115, 600
Bsp1720I GCTNAGC 1 cut(s) 291
BspACI CCGC 5 cut(s) 95, 288, 310, 389, 503
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 5 cut(s) 28, 44, 190, 304, 342
BspFNI CGCG 1 cut(s) 575
BspHI TCATGA 1 cut(s) 118
BspPI GGATC 1 cut(s) 595
BspQI GCTCTTC 1 cut(s) 798
BspTNI GGTCTC 1 cut(s) 506
BsrBI CCGCTC 1 cut(s) 290
BsrDI GCAATG 1 cut(s) 480
BsrFI RCCGGY 1 cut(s) 204
BssAI RCCGGY 1 cut(s) 204
BssECI CCNNGG 1 cut(s) 825
BssMI GATC 3 cut(s) 81, 115, 600
BssNI GRCGYC 1 cut(s) 419
BssSI CACGAG 1 cut(s) 646
BssT1I CCWWGG 1 cut(s) 825
Bst2BI CACGAG 1 cut(s) 646
Bst4CI ACNGT 1 cut(s) 556
Bst6I CTCTTC 1 cut(s) 798
BstACI GRCGYC 1 cut(s) 419
BstAPI GCANNNNNTGC 1 cut(s) 670
BstC8I GCNNGC 3 cut(s) 387, 743, 835
BstDEI CTNAG 5 cut(s) 15, 31, 177, 291, 350
BstFNI CGCG 1 cut(s) 575
BstHHI GCGC 1 cut(s) 577
BstKTI GATC 3 cut(s) 84, 118, 603
BstMAI GTCTC 4 cut(s) 229, 506, 512, 542
BstMBI GATC 3 cut(s) 81, 115, 600
BstMWI GCNNNNNNNGC 7 cut(s) 11, 51, 287, 301, 376, 626, 670
BstSCI CCNGG 1 cut(s) 107
BstUI CGCG 1 cut(s) 575
BstV1I GCAGC 6 cut(s) 175, 621, 670, 673, 698, 791
BsuRI GGCC 1 cut(s) 536
BtrI CACGTC 1 cut(s) 522
Cac8I GCNNGC 3 cut(s) 387, 743, 835
CciI TCATGA 1 cut(s) 118
CfoI GCGC 1 cut(s) 577
Cfr10I RCCGGY 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 562
CspAI ACCGGT 1 cut(s) 204
CviAII CATG 4 cut(s) 22, 46, 119, 838
DdeI CTNAG 5 cut(s) 15, 31, 177, 291, 350
DpnI GATC 3 cut(s) 83, 117, 602
DpnII GATC 3 cut(s) 81, 115, 600
EaeI YGGCCR 1 cut(s) 534
Eam1104I CTCTTC 1 cut(s) 798
EarI CTCTTC 1 cut(s) 798
Eco130I CCWWGG 1 cut(s) 825
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 506
Eco47I GGWCC 1 cut(s) 562
Eco57I CTGAAG 1 cut(s) 314
EcoT14I CCWWGG 1 cut(s) 825
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 825
Esp3I CGTCTC 2 cut(s) 512, 542
FaeI CATG 4 cut(s) 25, 49, 122, 841
FaiI YATR 4 cut(s) 23, 47, 120, 839
FalI AAGNNNNNCTT 2 cut(s) 780, 812
FatI CATG 4 cut(s) 21, 45, 118, 837
FauI CCCGC 1 cut(s) 303
FbaI TGATCA 2 cut(s) 81, 115
Fnu4HI GCNGC 7 cut(s) 164, 390, 610, 659, 662, 687, 805
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 7 cut(s) 164, 390, 610, 659, 662, 687, 805
FspBI CTAG 2 cut(s) 194, 729
GlaI GCGC 1 cut(s) 576
GluI GCNGC 7 cut(s) 164, 390, 610, 659, 662, 687, 805
GsaI CCCAGC 1 cut(s) 704
HaeIII GGCC 1 cut(s) 536
HapII CCGG 3 cut(s) 108, 132, 205
HhaI GCGC 1 cut(s) 577
Hin1I GRCGYC 1 cut(s) 419
Hin1II CATG 4 cut(s) 25, 49, 122, 841
Hin6I GCGC 1 cut(s) 575
HinP1I GCGC 1 cut(s) 575
HinfI GANTC 3 cut(s) 104, 410, 632
HpaII CCGG 3 cut(s) 108, 132, 205
HphI GGTGA 1 cut(s) 568
Hpy188I TCNGA 5 cut(s) 18, 34, 81, 103, 285
Hpy188III TCNNGA 6 cut(s) 119, 194, 275, 485, 678, 797
Hpy99I CGWCG 2 cut(s) 424, 556
HpyAV CCTTC 3 cut(s) 263, 646, 726
HpyCH4III ACNGT 1 cut(s) 556
HpyCH4IV ACGT 2 cut(s) 419, 521
HpyCH4V TGCA 9 cut(s) 243, 304, 324, 337, 364, 609, 629, 759, 777
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 51, 287, 301, 376, 626, 670
HpyF3I CTNAG 5 cut(s) 15, 31, 177, 291, 350
HpySE526I ACGT 2 cut(s) 419, 521
Hsp92I GRCGYC 1 cut(s) 419
Hsp92II CATG 4 cut(s) 25, 49, 122, 841
HspAI GCGC 1 cut(s) 575
Ksp22I TGATCA 2 cut(s) 81, 115
Kzo9I GATC 3 cut(s) 81, 115, 600
LguI GCTCTTC 1 cut(s) 798
LmnI GCTCC 3 cut(s) 178, 445, 478
LpnPI CCDG 9 cut(s) 53, 121, 145, 218, 371, 569, 617, 663, 686
Lsp1109I GCAGC 6 cut(s) 175, 621, 670, 673, 698, 791
LweI GCATC 3 cut(s) 144, 268, 616
MaeI CTAG 2 cut(s) 194, 729
MaeII ACGT 2 cut(s) 419, 521
MalI GATC 3 cut(s) 83, 117, 602
MbiI CCGCTC 1 cut(s) 290
MboI GATC 3 cut(s) 81, 115, 600
MboII GAAGA 4 cut(s) 210, 320, 743, 785
MhlI GDGCHC 2 cut(s) 79, 450
MlsI TGGCCA 1 cut(s) 536
MluCI AATT 2 cut(s) 37, 232
MluNI TGGCCA 1 cut(s) 536
MlyI GAGTC 2 cut(s) 419, 641
Mox20I TGGCCA 1 cut(s) 536
MroXI GAANNNNTTC 1 cut(s) 792
MscI TGGCCA 1 cut(s) 536
Msp20I TGGCCA 1 cut(s) 536
MspA1I CMGCKG 1 cut(s) 385
MspI CCGG 3 cut(s) 108, 132, 205
MspR9I CCNGG 1 cut(s) 109
Mva1269I GAATGC 1 cut(s) 324
MvnI CGCG 1 cut(s) 575
MwoI GCNNNNNNNGC 7 cut(s) 11, 51, 287, 301, 376, 626, 670
NciI CCSGG 1 cut(s) 109
NdeII GATC 3 cut(s) 81, 115, 600
NlaIII CATG 4 cut(s) 25, 49, 122, 841
PagI TCATGA 1 cut(s) 118
PciSI GCTCTTC 1 cut(s) 798
PcsI WCGNNNNNNNCGW 1 cut(s) 419
PctI GAATGC 1 cut(s) 324
PdmI GAANNNNTTC 1 cut(s) 792
PfeI GAWTC 1 cut(s) 104
PinAI ACCGGT 1 cut(s) 204
PkrI GCNGC 7 cut(s) 165, 391, 611, 660, 663, 688, 806
PleI GAGTC 2 cut(s) 418, 640
PpsI GAGTC 2 cut(s) 418, 640
PspFI CCCAGC 1 cut(s) 700
PspPI GGNCC 1 cut(s) 562
PvuII CAGCTG 1 cut(s) 385
SapI GCTCTTC 1 cut(s) 798
SatI GCNGC 7 cut(s) 164, 390, 610, 659, 662, 687, 805
Sau3AI GATC 3 cut(s) 81, 115, 600
Sau96I GGNCC 1 cut(s) 562
SchI GAGTC 2 cut(s) 419, 641
ScrFI CCNGG 1 cut(s) 109
SduI GDGCHC 2 cut(s) 79, 450
SfaNI GCATC 3 cut(s) 144, 268, 616
SinI GGWCC 1 cut(s) 562
SmlI CTYRAG 1 cut(s) 493
SmoI CTYRAG 1 cut(s) 493
Sse9I AATT 2 cut(s) 37, 232
SsiI CCGC 5 cut(s) 95, 288, 310, 389, 503
SspMI CTAG 2 cut(s) 194, 729
StyD4I CCNGG 1 cut(s) 107
StyI CCWWGG 1 cut(s) 825
TaaI ACNGT 1 cut(s) 556
TaiI ACGT 2 cut(s) 422, 524
TaqI TCGA 4 cut(s) 408, 413, 422, 579
TaqII GACCGA 1 cut(s) 530
TasI AATT 2 cut(s) 37, 232
TauI GCSGC 1 cut(s) 392
TfiI GAWTC 1 cut(s) 104
TseI GCWGC 6 cut(s) 163, 609, 658, 661, 686, 804
VpaK11BI GGWCC 1 cut(s) 562
XapI RAATTY 1 cut(s) 37
XbaI TCTAGA 1 cut(s) 193
XmnI GAANNNNTTC 1 cut(s) 792
XspI CTAG 2 cut(s) 194, 729
ZraI GACGTC 1 cut(s) 420
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.