Rh6CG010000

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0003008
Physical Location & Seq
Reverse (-)
1177410 .. 1179709
2300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG010000.1

Sequence Viewer

Length: 834 bp
ATGGCTATTGAAGCGCAGTTGTATTCAGAGAATCTTGGGTTTCCGATGTTTGGCTCACAATCGCAGAATTTGATGGTGGACAACAATGGTTGTGGTGGTTCTGGCGGTGGGTTTAATCAGTTCTGTTTCAATGTCCAGCAGAAACCGCCGCAGCTACTACAATACCAGCCACCACCGATGAATCCCGGTGTGCTTGTCCCAGCTTTGCACAACAACAACATCATGGCTTATACTCGACAGAGTATTGCAGCTCAGGAAGCCAAGCAAAGACAAGAGATTGATGACTATCTCAGATTACAGAATGAGAGGCTGAGATTGGTGCTACAAGAGCAGAGAAAGCAGCAACTCGCATTGTTCATGAAGAAAATCGAGTCCAGAACAGAGCTACTATTGAAGCAAAAGGATGAAGAAATAGCACAAGCGGTGAAGAAGAGAATGGAGCTCGAAGATTTCTTGAGGAAATTGGAGGCTGAAAACCAAGCGTGGCAAAGAGTGGCTCAGGAGAACGAAGCCATGGTTGTGTCCTTAAACAACACTCTTGAACAGTTCAGGGAGAGAGCCGCTTGTGGTGCCATGGAAGATGCACAGTCTTGCTGTGATAGTAAACAAGAGGAGGAAGAAGAAGACACAGGGGATTACAGAGGAGGCTTTAATAATGGCGTGAATGAAGAGATAAAGATGATGGTTTGCAAAGGCTGCAATTCTAAAAGGTCGTGTGTCTTGTTCCTCCCCTGCAGGCATCTTTGTTCATGCAAAGCCTGTGAGGCTGGCTTTGATTCTTGTCCTGTTTGCACAACGCCAAAGAAGGCTAGCATAGAGGCATTGATTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008589 GO:0009966 GO:0009968 GO:0010646 GO:0010648 GO:0023051 GO:0023057 GO:0045879 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

277

Amino Acids

31.35

Weight (kDa)

5.26

Isoelectric Point (pI)

72.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 228 - 269 7.6e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 569
AciI CCGC 5 cut(s) 105, 146, 149, 422, 561
AcsI RAATTY 1 cut(s) 67
AfiI CCNNNNNNNGG 1 cut(s) 50
AgsI TTSAA 4 cut(s) 11, 130, 394, 542
AluBI AGCT 5 cut(s) 154, 203, 251, 385, 442
AluI AGCT 5 cut(s) 154, 203, 251, 385, 442
Alw21I GWGCWC 1 cut(s) 444
ApeKI GCWGC 4 cut(s) 151, 248, 340, 696
ApoI RAATTY 1 cut(s) 67
AspLEI GCGC 1 cut(s) 16
AsuC2I CCSGG 1 cut(s) 186
AsuHPI GGTGA 1 cut(s) 436
AsuNHI GCTAGC 1 cut(s) 809
BanI GGYRCC 1 cut(s) 569
BanII GRGCYC 1 cut(s) 444
BbsI GAAGAC 1 cut(s) 630
Bbv12I GWGCWC 1 cut(s) 444
BbvI GCAGC 4 cut(s) 163, 260, 352, 683
BccI CCATC 2 cut(s) 67, 676
BcnI CCSGG 1 cut(s) 186
BfaI CTAG 2 cut(s) 810, 832
BfmI CTRYAG 1 cut(s) 733
BglI GCCNNNNNGGC 1 cut(s) 764
BisI GCNGC 6 cut(s) 149, 152, 249, 341, 561, 697
BlsI GCNGC 6 cut(s) 150, 153, 250, 342, 562, 698
Bme1390I CCNGG 1 cut(s) 186
BmiI GGNNCC 1 cut(s) 571
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 571, 748
BmtI GCTAGC 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 630
Bpu10I CCTNAGC 2 cut(s) 252, 498
BpuEI CTTGAG 1 cut(s) 475
BpuMI CCSGG 1 cut(s) 186
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 513, 573
Bsc4I CCNNNNNNNGG 1 cut(s) 50
Bse8I GATNNNNATC 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 513, 573
BseGI GGATG 1 cut(s) 409
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 1 cut(s) 50
BseMII CTCAG 4 cut(s) 266, 302, 304, 512
BseRI GAGGAG 2 cut(s) 626, 657
BseXI GCAGC 4 cut(s) 163, 260, 352, 683
BseYI CCCAGC 1 cut(s) 199
BshNI GGYRCC 1 cut(s) 569
BsiHKAI GWGCWC 1 cut(s) 444
BsiSI CCGG 1 cut(s) 186
BslFI GGGAC 1 cut(s) 182
BslI CCNNNNNNNGG 1 cut(s) 50
BsmFI GGGAC 1 cut(s) 182
Bsp1286I GDGCHC 1 cut(s) 444
Bsp19I CCATGG 2 cut(s) 513, 573
BspACI CCGC 5 cut(s) 105, 146, 149, 422, 561
BspCNI CTCAG 4 cut(s) 265, 303, 303, 511
BspHI TCATGA 1 cut(s) 357
BspLI GGNNCC 1 cut(s) 571
BspMAI CTGCAG 1 cut(s) 737
BspOI GCTAGC 1 cut(s) 813
BspT107I GGYRCC 1 cut(s) 569
BssECI CCNNGG 2 cut(s) 513, 573
BssT1I CCWWGG 2 cut(s) 513, 573
Bst4CI ACNGT 2 cut(s) 546, 588
Bst6I CTCTTC 2 cut(s) 425, 663
BstAPI GCANNNNNTGC 1 cut(s) 696
BstC8I GCNNGC 3 cut(s) 737, 769, 811
BstDEI CTNAG 4 cut(s) 252, 290, 311, 498
BstDSI CCRYGG 2 cut(s) 513, 573
BstF5I GGATG 1 cut(s) 409
BstHHI GCGC 1 cut(s) 16
BstMWI GCNNNNNNNGC 8 cut(s) 11, 145, 257, 328, 337, 569, 696, 764
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 733
BstV1I GCAGC 4 cut(s) 163, 260, 352, 683
BstV2I GAAGAC 1 cut(s) 630
BtgI CCRYGG 2 cut(s) 513, 573
BtsCI GGATG 1 cut(s) 409
Cac8I GCNNGC 3 cut(s) 737, 769, 811
CciI TCATGA 1 cut(s) 357
CfoI GCGC 1 cut(s) 16
CspCI CAANNNNNGTGG 2 cut(s) 73, 108
CviAII CATG 5 cut(s) 223, 358, 514, 574, 750
DdeI CTNAG 4 cut(s) 252, 290, 311, 498
Eam1104I CTCTTC 2 cut(s) 425, 663
EarI CTCTTC 2 cut(s) 425, 663
Ecl136II GAGCTC 1 cut(s) 442
Eco130I CCWWGG 2 cut(s) 513, 573
Eco24I GRGCYC 1 cut(s) 444
Eco53kI GAGCTC 1 cut(s) 442
EcoICRI GAGCTC 1 cut(s) 442
EcoT14I CCWWGG 2 cut(s) 513, 573
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 2 cut(s) 513, 573
FaeI CATG 5 cut(s) 226, 361, 517, 577, 753
FaiI YATR 7 cut(s) 224, 231, 359, 515, 575, 751, 815
FaqI GGGAC 1 cut(s) 182
FatI CATG 5 cut(s) 222, 357, 513, 573, 749
Fnu4HI GCNGC 6 cut(s) 149, 152, 249, 341, 561, 697
FokI GGATG 1 cut(s) 416
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 6 cut(s) 149, 152, 249, 341, 561, 697
FspBI CTAG 2 cut(s) 810, 832
GlaI GCGC 1 cut(s) 15
GluI GCNGC 6 cut(s) 149, 152, 249, 341, 561, 697
GsaI CCCAGC 1 cut(s) 203
HapII CCGG 1 cut(s) 186
HhaI GCGC 1 cut(s) 16
Hin1II CATG 5 cut(s) 226, 361, 517, 577, 753
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HinfI GANTC 4 cut(s) 31, 181, 371, 776
HpaII CCGG 1 cut(s) 186
HphI GGTGA 1 cut(s) 436
Hpy166II GTNNAC 2 cut(s) 79, 605
Hpy188I TCNGA 3 cut(s) 28, 45, 293
Hpy188III TCNNGA 6 cut(s) 254, 358, 375, 454, 500, 539
Hpy8I GTNNAC 2 cut(s) 79, 605
HpyAV CCTTC 1 cut(s) 799
HpyCH4III ACNGT 2 cut(s) 546, 588
HpyCH4V TGCA 8 cut(s) 208, 248, 584, 690, 699, 735, 753, 792
HpyF10VI GCNNNNNNNGC 8 cut(s) 11, 145, 257, 328, 337, 569, 696, 764
HpyF3I CTNAG 4 cut(s) 252, 290, 311, 498
Hsp92II CATG 5 cut(s) 226, 361, 517, 577, 753
HspAI GCGC 1 cut(s) 14
LmnI GCTCC 1 cut(s) 439
Lsp1109I GCAGC 4 cut(s) 163, 260, 352, 683
LweI GCATC 2 cut(s) 571, 748
MaeI CTAG 2 cut(s) 810, 832
MhlI GDGCHC 1 cut(s) 444
MluCI AATT 3 cut(s) 67, 461, 700
MlyI GAGTC 1 cut(s) 380
MseI TTAA 3 cut(s) 114, 527, 651
MslI CAYNNNNRTG 1 cut(s) 518
MspI CCGG 1 cut(s) 186
MspR9I CCNGG 1 cut(s) 186
MwoI GCNNNNNNNGC 8 cut(s) 11, 145, 257, 328, 337, 569, 696, 764
NciI CCSGG 1 cut(s) 186
NcoI CCATGG 2 cut(s) 513, 573
NheI GCTAGC 1 cut(s) 809
NlaIII CATG 5 cut(s) 226, 361, 517, 577, 753
NlaIV GGNNCC 1 cut(s) 571
PagI TCATGA 1 cut(s) 357
PfeI GAWTC 3 cut(s) 31, 181, 776
PkrI GCNGC 6 cut(s) 150, 153, 250, 342, 562, 698
PleI GAGTC 1 cut(s) 379
PpsI GAGTC 1 cut(s) 379
Psp124BI GAGCTC 1 cut(s) 444
PspFI CCCAGC 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 571
PstI CTGCAG 1 cut(s) 737
RseI CAYNNNNRTG 1 cut(s) 518
SacI GAGCTC 1 cut(s) 444
SaqAI TTAA 3 cut(s) 114, 527, 651
SatI GCNGC 6 cut(s) 149, 152, 249, 341, 561, 697
SbfI CCTGCAGG 1 cut(s) 737
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 186
SdaI CCTGCAGG 1 cut(s) 737
SduI GDGCHC 1 cut(s) 444
SetI ASST 6 cut(s) 156, 205, 253, 387, 444, 713
SfaNI GCATC 2 cut(s) 571, 748
SfcI CTRYAG 1 cut(s) 733
SmiMI CAYNNNNRTG 1 cut(s) 518
SmlI CTYRAG 1 cut(s) 454
SmoI CTYRAG 1 cut(s) 454
Sse8387I CCTGCAGG 1 cut(s) 737
Sse9I AATT 3 cut(s) 67, 461, 700
SsiI CCGC 5 cut(s) 105, 146, 149, 422, 561
SspMI CTAG 2 cut(s) 810, 832
SstI GAGCTC 1 cut(s) 444
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 2 cut(s) 513, 573
TaaI ACNGT 2 cut(s) 546, 588
TaqI TCGA 3 cut(s) 235, 369, 444
TasI AATT 3 cut(s) 67, 461, 700
TauI GCSGC 2 cut(s) 151, 563
TfiI GAWTC 3 cut(s) 31, 181, 776
Tru1I TTAA 3 cut(s) 114, 527, 651
Tru9I TTAA 3 cut(s) 114, 527, 651
TseI GCWGC 4 cut(s) 151, 248, 340, 696
TspDTI ATGAA 6 cut(s) 194, 346, 374, 420, 681, 738
XapI RAATTY 1 cut(s) 67
XspI CTAG 2 cut(s) 810, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.