RchiOBHm_Chr6g0303341

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003008
Physical Location & Seq
Forward (+)
63086477 .. 63087821
1345 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27251

Sequence Viewer

Length: 861 bp
ATGGCAGTTCAAGCTCAGACCATGTATTTCTCAGAAAATTTAGGCGTGAGTGGCTTTCCACAGCAACAGGATTGGGCTCCGATCAACCCTCCTCATCCGCTTTCCGATTCCGGGTTTGATCATGACTTCTTTTCCGGCATCATTCCAAACCCTCAACCTTATCAGCAGCAACAAGCTCCTCAGTTTCATCAAAATCTAGAAGCTAACCGGTTAGGAGTTTCTTCTGTTTCTCCTAATTCCACTTGCAATAGCTTCCTTCCCACAGCGTCATTATTTCCCGATACTCTGACAGCTCAGCTCGACTTGCAAAGGCGGGAGATAGATTGCATTCTTCAGTTGCAGAATGAGAAGCTGAGATTTGCATTGCAAGAGCAGAGGAAGGGACAGCTGGCGGCTCTGTTGAGCAACTTCGAGTCGAGGACGTCGAGTTTGATGAGGCAAAAAGAGGAGCACTTATCACAAGCGAGAAAGAGAGCAATGGAGCTTCAAGATTGCTTGAGAAAAGCGGAGATGGAGACCGAGACGTGGCAGAGATTGGCCAAAGCAAACGAGACGACGGTGATGGACCTCAACAACGCGCTCGAACAGGTCAAAGAGAGAATGGTCCTGTGCAGCAACGAAGCCGATGATGCAGAGTCCTTCTGTGGCTCTTGTGAGAAGCAGCAGCAACAAGTGCTTTCAGAAAAAGTAGCTCACAAAAAGCTGGGTTTGGATTGTAAAAGCTGTAATGCTAGAAGGTCGTGCGTGCTTTTTCTTCCTTGCAGACACCTCTGTTCGTGCAAGTTTTGTGAAGCTTTTCTTGATTGCTGCCCCGTGTGTAAATCCACCAAGGAGGCAAGCATGGAGGTCTTTTTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

286

Amino Acids

32.38

Weight (kDa)

5.75

Isoelectric Point (pI)

54.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 238 - 278 2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 425
AccII CGCG 1 cut(s) 578
AciI CCGC 4 cut(s) 98, 313, 392, 506
AcoI YGGCCR 1 cut(s) 537
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 317
AcyI GRCGYC 1 cut(s) 422
AfiI CCNNNNNNNGG 2 cut(s) 111, 525
AgeI ACCGGT 1 cut(s) 207
AgsI TTSAA 2 cut(s) 11, 488
AjiI CACGTC 1 cut(s) 525
Alw21I GWGCWC 1 cut(s) 453
Alw26I GTCTC 3 cut(s) 509, 515, 545
AoxI GGCC 1 cut(s) 537
ApeKI GCWGC 5 cut(s) 166, 612, 661, 664, 807
ApoI RAATTY 1 cut(s) 37
ArsI GACNNNNNNTTYG 2 cut(s) 412, 444
AsiGI ACCGGT 1 cut(s) 207
Asp700I GAANNNNTTC 1 cut(s) 795
AspLEI GCGC 1 cut(s) 580
AspS9I GGNCC 2 cut(s) 565, 604
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 571
AvaII GGWCC 2 cut(s) 565, 604
BalI TGGCCA 1 cut(s) 539
BanII GRGCYC 1 cut(s) 79
Bbv12I GWGCWC 1 cut(s) 453
BbvI GCAGC 5 cut(s) 178, 624, 673, 676, 794
BccI CCATC 2 cut(s) 505, 556
BclI TGATCA 1 cut(s) 118
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 3 cut(s) 509, 515, 545
BfaI CTAG 2 cut(s) 197, 732
BisI GCNGC 6 cut(s) 167, 393, 613, 662, 665, 808
BlpI GCTNAGC 1 cut(s) 294
BlsI GCNGC 6 cut(s) 168, 394, 614, 663, 666, 809
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 2 cut(s) 565, 604
BmgBI CACGTC 1 cut(s) 525
BmgT120I GGNCC 2 cut(s) 565, 604
BmiI GGNNCC 1 cut(s) 78
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 2 cut(s) 147, 619
Bpu1102I GCTNAGC 1 cut(s) 294
BpuEI CTTGAG 1 cut(s) 517
BpuMI CCSGG 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 509
BsaJI CCNNGG 1 cut(s) 828
BsaWI WCCGGW 1 cut(s) 207
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 525
Bse118I RCCGGY 1 cut(s) 207
Bse3DI GCAATG 2 cut(s) 362, 483
BseDI CCNNGG 1 cut(s) 828
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 111, 525
BseMI GCAATG 2 cut(s) 362, 483
BseMII CTCAG 5 cut(s) 29, 45, 194, 308, 344
BseRI GAGGAG 3 cut(s) 81, 168, 461
BseXI GCAGC 5 cut(s) 178, 624, 673, 676, 794
BseYI CCCAGC 1 cut(s) 703
BsgI GTGCAG 1 cut(s) 631
Bsh1236I CGCG 1 cut(s) 578
BshFI GGCC 1 cut(s) 539
BshTI ACCGGT 1 cut(s) 207
BsiHKAI GWGCWC 1 cut(s) 453
BsiSI CCGG 3 cut(s) 111, 135, 208
BslFI GGGAC 1 cut(s) 396
BslI CCNNNNNNNGG 2 cut(s) 111, 525
BsmAI GTCTC 3 cut(s) 509, 515, 545
BsmBI CGTCTC 2 cut(s) 515, 545
BsmFI GGGAC 1 cut(s) 396
BsmI GAATGC 1 cut(s) 327
BsnI GGCC 1 cut(s) 539
Bso31I GGTCTC 1 cut(s) 509
Bsp1286I GDGCHC 2 cut(s) 79, 453
Bsp143I GATC 2 cut(s) 81, 118
Bsp1720I GCTNAGC 1 cut(s) 294
BspACI CCGC 4 cut(s) 98, 313, 392, 506
BspANI GGCC 1 cut(s) 539
BspCNI CTCAG 5 cut(s) 28, 44, 193, 307, 345
BspFNI CGCG 1 cut(s) 578
BspHI TCATGA 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 78
BspTNI GGTCTC 1 cut(s) 509
BsrDI GCAATG 2 cut(s) 362, 483
BsrFI RCCGGY 1 cut(s) 207
BssAI RCCGGY 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 828
BssMI GATC 2 cut(s) 81, 118
BssNI GRCGYC 1 cut(s) 422
BssT1I CCWWGG 1 cut(s) 828
Bst4CI ACNGT 1 cut(s) 559
BstACI GRCGYC 1 cut(s) 422
BstAPI GCANNNNNTGC 1 cut(s) 673
BstC8I GCNNGC 3 cut(s) 390, 746, 838
BstDEI CTNAG 5 cut(s) 15, 31, 180, 294, 353
BstF5I GGATG 1 cut(s) 94
BstFNI CGCG 1 cut(s) 578
BstHHI GCGC 1 cut(s) 580
BstKTI GATC 2 cut(s) 84, 121
BstMAI GTCTC 3 cut(s) 509, 515, 545
BstMBI GATC 2 cut(s) 81, 118
BstMWI GCNNNNNNNGC 5 cut(s) 11, 51, 304, 629, 673
BstSCI CCNGG 1 cut(s) 110
BstUI CGCG 1 cut(s) 578
BstV1I GCAGC 5 cut(s) 178, 624, 673, 676, 794
BsuRI GGCC 1 cut(s) 539
BtrI CACGTC 1 cut(s) 525
BtsCI GGATG 1 cut(s) 94
Cac8I GCNNGC 3 cut(s) 390, 746, 838
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 580
Cfr10I RCCGGY 1 cut(s) 207
Cfr13I GGNCC 2 cut(s) 565, 604
CseI GACGC 1 cut(s) 255
CspAI ACCGGT 1 cut(s) 207
CviAII CATG 3 cut(s) 22, 122, 841
DdeI CTNAG 5 cut(s) 15, 31, 180, 294, 353
DpnI GATC 2 cut(s) 83, 120
DpnII GATC 2 cut(s) 81, 118
EaeI YGGCCR 1 cut(s) 537
Eco130I CCWWGG 1 cut(s) 828
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 509
Eco47I GGWCC 2 cut(s) 565, 604
Eco57I CTGAAG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 828
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 828
Esp3I CGTCTC 2 cut(s) 515, 545
FaeI CATG 3 cut(s) 25, 125, 844
FaiI YATR 3 cut(s) 23, 123, 842
FalI AAGNNNNNCTT 2 cut(s) 783, 815
FaqI GGGAC 1 cut(s) 396
FatI CATG 3 cut(s) 21, 121, 840
FauI CCCGC 1 cut(s) 306
FbaI TGATCA 1 cut(s) 118
Fnu4HI GCNGC 6 cut(s) 167, 393, 613, 662, 665, 808
FokI GGATG 1 cut(s) 81
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 6 cut(s) 167, 393, 613, 662, 665, 808
FspBI CTAG 2 cut(s) 197, 732
GlaI GCGC 1 cut(s) 579
GluI GCNGC 6 cut(s) 167, 393, 613, 662, 665, 808
GsaI CCCAGC 1 cut(s) 707
HaeIII GGCC 1 cut(s) 539
HapII CCGG 3 cut(s) 111, 135, 208
HgaI GACGC 1 cut(s) 255
HhaI GCGC 1 cut(s) 580
Hin1I GRCGYC 1 cut(s) 422
Hin1II CATG 3 cut(s) 25, 125, 844
Hin6I GCGC 1 cut(s) 578
HinP1I GCGC 1 cut(s) 578
HindIII AAGCTT 1 cut(s) 792
HinfI GANTC 3 cut(s) 107, 413, 635
HpaII CCGG 3 cut(s) 111, 135, 208
HphI GGTGA 1 cut(s) 571
Hpy188I TCNGA 6 cut(s) 18, 34, 81, 106, 288, 682
Hpy188III TCNNGA 5 cut(s) 122, 197, 278, 488, 800
Hpy99I CGWCG 2 cut(s) 427, 559
HpyAV CCTTC 4 cut(s) 266, 373, 649, 729
HpyCH4III ACNGT 1 cut(s) 559
HpyCH4IV ACGT 2 cut(s) 422, 524
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 51, 304, 629, 673
HpyF3I CTNAG 5 cut(s) 15, 31, 180, 294, 353
HpySE526I ACGT 2 cut(s) 422, 524
Hsp92I GRCGYC 1 cut(s) 422
Hsp92II CATG 3 cut(s) 25, 125, 844
HspAI GCGC 1 cut(s) 578
Ksp22I TGATCA 1 cut(s) 118
Kzo9I GATC 2 cut(s) 81, 118
LmnI GCTCC 4 cut(s) 82, 181, 448, 481
LpnPI CCDG 8 cut(s) 53, 124, 148, 221, 374, 572, 620, 689
Lsp1109I GCAGC 5 cut(s) 178, 624, 673, 676, 794
LweI GCATC 2 cut(s) 147, 619
MaeI CTAG 2 cut(s) 197, 732
MaeII ACGT 2 cut(s) 422, 524
MalI GATC 2 cut(s) 83, 120
MboI GATC 2 cut(s) 81, 118
MboII GAAGA 3 cut(s) 213, 323, 746
MhlI GDGCHC 2 cut(s) 79, 453
MlsI TGGCCA 1 cut(s) 539
MluCI AATT 2 cut(s) 37, 235
MluNI TGGCCA 1 cut(s) 539
MlyI GAGTC 2 cut(s) 422, 644
Mox20I TGGCCA 1 cut(s) 539
MroXI GAANNNNTTC 1 cut(s) 795
MscI TGGCCA 1 cut(s) 539
Msp20I TGGCCA 1 cut(s) 539
MspA1I CMGCKG 1 cut(s) 388
MspI CCGG 3 cut(s) 111, 135, 208
MspR9I CCNGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 327
MvnI CGCG 1 cut(s) 578
MwoI GCNNNNNNNGC 5 cut(s) 11, 51, 304, 629, 673
NciI CCSGG 1 cut(s) 112
NdeII GATC 2 cut(s) 81, 118
NlaIII CATG 3 cut(s) 25, 125, 844
NlaIV GGNNCC 1 cut(s) 78
PagI TCATGA 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 422
PctI GAATGC 1 cut(s) 327
PdmI GAANNNNTTC 1 cut(s) 795
PfeI GAWTC 1 cut(s) 107
PinAI ACCGGT 1 cut(s) 207
PkrI GCNGC 6 cut(s) 168, 394, 614, 663, 666, 809
PleI GAGTC 2 cut(s) 421, 643
PpsI GAGTC 2 cut(s) 421, 643
PspFI CCCAGC 1 cut(s) 703
PspN4I GGNNCC 1 cut(s) 78
PspPI GGNCC 2 cut(s) 565, 604
PvuII CAGCTG 1 cut(s) 388
SatI GCNGC 6 cut(s) 167, 393, 613, 662, 665, 808
Sau3AI GATC 2 cut(s) 81, 118
Sau96I GGNCC 2 cut(s) 565, 604
SchI GAGTC 2 cut(s) 422, 644
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 2 cut(s) 79, 453
SfaNI GCATC 2 cut(s) 147, 619
SinI GGWCC 2 cut(s) 565, 604
SmlI CTYRAG 1 cut(s) 496
SmoI CTYRAG 1 cut(s) 496
Sse9I AATT 2 cut(s) 37, 235
SsiI CCGC 4 cut(s) 98, 313, 392, 506
SspMI CTAG 2 cut(s) 197, 732
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 828
TaaI ACNGT 1 cut(s) 559
TaiI ACGT 2 cut(s) 425, 527
TaqI TCGA 5 cut(s) 300, 411, 416, 425, 582
TaqII GACCGA 1 cut(s) 533
TasI AATT 2 cut(s) 37, 235
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 1 cut(s) 107
TseI GCWGC 5 cut(s) 166, 612, 661, 664, 807
TspDTI ATGAA 1 cut(s) 176
VpaK11BI GGWCC 2 cut(s) 565, 604
XapI RAATTY 1 cut(s) 37
XbaI TCTAGA 1 cut(s) 196
XmnI GAANNNNTTC 1 cut(s) 795
XspI CTAG 2 cut(s) 197, 732
ZraI GACGTC 1 cut(s) 423
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.