FvH4_2g38140

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003008
Physical Location & Seq
Reverse (-)
27631235 .. 27632788
1554 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38140.t1

Sequence Viewer

Length: 846 bp
ATGGCAGTTCAAGCTCAGACCATGTATTTCTCAGAAAACTTGGGCATGAGTGGCTTCCCGCAGCAACAAGATTGGTCTCTGATCAACCCACCTCCTCTTTCCGATCCCGGGTTTGATCATGACTTCTTCTCCGGCCTCATTCCAGACCCTCAAACTCATCAGCAGCAACAAGTTCATCAGTTTCATCCAAATCTAGGAGTTTCTTCTGTTTCAAATTCTCCTAATTCCACTTGCAATAGCTTCCTCCCCACGACGTCGTTTTCCGATACTCTCACCGCTCAGCTCGACTTACAAAGGCAGGAGATAGATTGCATTCTTCAGTTGCAGAATGAGAAGCTGAGATTTGCTTTGCAAGAGCAGAGGAAGCGGCAGCTGGCTGCCCTGTTGAGAAACTTCGAGTCTAGGACGTCGAGTTTGATCAGGCAAAAAGAGGAGCACTTGTCACAAGCGAGAAAGAGAGCAATGGAGCTTCAAGATTGCTTGAGGAAGGCGGAGATGGAGACGGAGACGTGGCAGAGATTGGCCAAAGCAAACGAGACGATGGTGATGGACCTCAACAACACGCTCGAACAGGTCAAAGAGAGAAGGGTCCTTTGCAGCAACGGAACCGATGATGCAGAGTCCTTCTGTGGCTCATCTTGCGAGAAGCAAGAAGTGCTTTCAGAAAAAGCAGTTCACAACAAAAAGCTGGGTTTGGATTGTAAAAGCTGTAATGCTAGAAGGTCGTGCGTGCTTTTTCTTCCTTGCAGACACCTCTGCTCGTGCAAGTTCTGTGAAGCTTTTCTTGATTATTGCCCCGTTTGTAAATCGACCAAGGAGGCAAGCATGGAGGTCTTTTTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

282

Amino Acids

32.03

Weight (kDa)

6.01

Isoelectric Point (pI)

59.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 233 - 273 1.7e-08 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 257, 410
AccBSI CCGCTC 1 cut(s) 278
AciI CCGC 4 cut(s) 59, 276, 367, 491
AclWI GGATC 1 cut(s) 98
AcoI YGGCCR 1 cut(s) 522
AcsI RAATTY 1 cut(s) 214
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 2 cut(s) 254, 407
AfiI CCNNNNNNNGG 2 cut(s) 108, 194
AgsI TTSAA 3 cut(s) 11, 213, 473
AjiI CACGTC 1 cut(s) 510
AluBI AGCT 9 cut(s) 14, 240, 283, 337, 373, 469, 688, 708, 779
AluI AGCT 9 cut(s) 14, 240, 283, 337, 373, 469, 688, 708, 779
Alw21I GWGCWC 1 cut(s) 438
Alw26I GTCTC 4 cut(s) 81, 494, 500, 530
AlwI GGATC 1 cut(s) 98
Ama87I CYCGRG 1 cut(s) 107
AoxI GGCC 2 cut(s) 133, 522
ApeKI GCWGC 5 cut(s) 61, 163, 370, 377, 597
ApoI RAATTY 1 cut(s) 214
ArsI GACNNNNNNTTYG 2 cut(s) 397, 429
Asp700I GAANNNNTTC 1 cut(s) 780
AspS9I GGNCC 2 cut(s) 550, 589
AsuC2I CCSGG 2 cut(s) 108, 109
AsuHPI GGTGA 2 cut(s) 265, 556
AvaI CYCGRG 1 cut(s) 107
AvaII GGWCC 2 cut(s) 550, 589
BalI TGGCCA 1 cut(s) 524
BauI CACGAG 1 cut(s) 760
Bbv12I GWGCWC 1 cut(s) 438
BbvI GCAGC 5 cut(s) 73, 175, 364, 382, 609
BccI CCATC 3 cut(s) 490, 535, 541
BclI TGATCA 3 cut(s) 81, 115, 417
BcnI CCSGG 2 cut(s) 108, 109
BcoDI GTCTC 4 cut(s) 81, 494, 500, 530
BfaI CTAG 3 cut(s) 194, 402, 717
BisI GCNGC 6 cut(s) 62, 164, 368, 371, 378, 598
BlpI GCTNAGC 1 cut(s) 279
BlsI GCNGC 6 cut(s) 63, 165, 369, 372, 379, 599
Bme1390I CCNGG 2 cut(s) 108, 109
Bme18I GGWCC 2 cut(s) 550, 589
BmeT110I CYCGRG 1 cut(s) 107
BmgBI CACGTC 1 cut(s) 510
BmgT120I GGNCC 2 cut(s) 550, 589
BmiI GGNNCC 2 cut(s) 590, 607
BmrFI CCNGG 2 cut(s) 108, 109
BmsI GCATC 1 cut(s) 604
Bpu1102I GCTNAGC 1 cut(s) 279
BpuEI CTTGAG 1 cut(s) 502
BpuMI CCSGG 2 cut(s) 108, 109
BsaHI GRCGYC 2 cut(s) 254, 407
BsaI GGTCTC 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 107, 813
BsaXI ACNNNNNCTCC 4 cut(s) 113, 143, 425, 455
Bsc4I CCNNNNNNNGG 2 cut(s) 108, 194
Bse3DI GCAATG 1 cut(s) 468
BseDI CCNNGG 2 cut(s) 107, 813
BseGI GGATG 1 cut(s) 184
BseLI CCNNNNNNNGG 2 cut(s) 108, 194
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 4 cut(s) 29, 45, 293, 329
BseRI GAGGAG 2 cut(s) 84, 446
BseXI GCAGC 5 cut(s) 73, 175, 364, 382, 609
BseYI CCCAGC 1 cut(s) 688
BshFI GGCC 2 cut(s) 135, 524
BsiHKAI GWGCWC 1 cut(s) 438
BsiHKCI CYCGRG 1 cut(s) 107
BsiSI CCGG 2 cut(s) 108, 132
BslI CCNNNNNNNGG 2 cut(s) 108, 194
BsmAI GTCTC 4 cut(s) 81, 494, 500, 530
BsmBI CGTCTC 3 cut(s) 494, 500, 530
BsmI GAATGC 1 cut(s) 312
BsnI GGCC 2 cut(s) 135, 524
Bso31I GGTCTC 1 cut(s) 81
BsoBI CYCGRG 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 438
Bsp143I GATC 4 cut(s) 81, 103, 115, 417
Bsp1720I GCTNAGC 1 cut(s) 279
BspACI CCGC 4 cut(s) 59, 276, 367, 491
BspANI GGCC 2 cut(s) 135, 524
BspCNI CTCAG 4 cut(s) 28, 44, 292, 330
BspHI TCATGA 1 cut(s) 118
BspLI GGNNCC 2 cut(s) 590, 607
BspPI GGATC 1 cut(s) 98
BspTNI GGTCTC 1 cut(s) 81
BsrBI CCGCTC 1 cut(s) 278
BsrDI GCAATG 1 cut(s) 468
BssECI CCNNGG 2 cut(s) 107, 813
BssMI GATC 4 cut(s) 81, 103, 115, 417
BssNI GRCGYC 2 cut(s) 254, 407
BssSI CACGAG 1 cut(s) 760
BssT1I CCWWGG 1 cut(s) 813
Bst2BI CACGAG 1 cut(s) 760
BstACI GRCGYC 2 cut(s) 254, 407
BstAPI GCANNNNNTGC 1 cut(s) 655
BstC8I GCNNGC 3 cut(s) 375, 731, 823
BstDEI CTNAG 4 cut(s) 15, 31, 279, 338
BstF5I GGATG 1 cut(s) 184
BstKTI GATC 4 cut(s) 84, 106, 118, 420
BstMAI GTCTC 4 cut(s) 81, 494, 500, 530
BstMBI GATC 4 cut(s) 81, 103, 115, 417
BstMWI GCNNNNNNNGC 5 cut(s) 11, 51, 364, 639, 655
BstSCI CCNGG 2 cut(s) 106, 107
BstV1I GCAGC 5 cut(s) 73, 175, 364, 382, 609
BsuRI GGCC 2 cut(s) 135, 524
BtrI CACGTC 1 cut(s) 510
BtsCI GGATG 1 cut(s) 184
Cac8I GCNNGC 3 cut(s) 375, 731, 823
CciI TCATGA 1 cut(s) 118
Cfr13I GGNCC 2 cut(s) 550, 589
Cfr9I CCCGGG 1 cut(s) 107
CviAII CATG 4 cut(s) 22, 46, 119, 826
DdeI CTNAG 4 cut(s) 15, 31, 279, 338
DpnI GATC 4 cut(s) 83, 105, 117, 419
DpnII GATC 4 cut(s) 81, 103, 115, 417
EaeI YGGCCR 1 cut(s) 522
EciI GGCGGA 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 813
Eco31I GGTCTC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 550, 589
Eco57I CTGAAG 1 cut(s) 302
Eco88I CYCGRG 1 cut(s) 107
EcoO109I RGGNCCY 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 813
ErhI CCWWGG 1 cut(s) 813
Esp3I CGTCTC 3 cut(s) 494, 500, 530
FaeI CATG 4 cut(s) 25, 49, 122, 829
FaiI YATR 4 cut(s) 23, 47, 120, 827
FalI AAGNNNNNCTT 4 cut(s) 642, 674, 768, 800
FatI CATG 4 cut(s) 21, 45, 118, 825
FauI CCCGC 1 cut(s) 66
FbaI TGATCA 3 cut(s) 81, 115, 417
Fnu4HI GCNGC 6 cut(s) 62, 164, 368, 371, 378, 598
FokI GGATG 1 cut(s) 171
Fsp4HI GCNGC 6 cut(s) 62, 164, 368, 371, 378, 598
FspBI CTAG 3 cut(s) 194, 402, 717
GluI GCNGC 6 cut(s) 62, 164, 368, 371, 378, 598
GsaI CCCAGC 1 cut(s) 692
HaeIII GGCC 2 cut(s) 135, 524
HapII CCGG 2 cut(s) 108, 132
Hin1I GRCGYC 2 cut(s) 254, 407
Hin1II CATG 4 cut(s) 25, 49, 122, 829
HindIII AAGCTT 1 cut(s) 777
HinfI GANTC 2 cut(s) 398, 620
HpaII CCGG 2 cut(s) 108, 132
HphI GGTGA 2 cut(s) 265, 556
Hpy166II GTNNAC 1 cut(s) 676
Hpy188I TCNGA 6 cut(s) 18, 34, 81, 103, 265, 664
Hpy188III TCNNGA 4 cut(s) 119, 143, 473, 785
Hpy8I GTNNAC 1 cut(s) 676
Hpy99I CGWCG 3 cut(s) 256, 259, 412
HpyAV CCTTC 4 cut(s) 481, 579, 634, 714
HpyCH4IV ACGT 3 cut(s) 254, 407, 509
HpyCH4V TGCA 8 cut(s) 234, 312, 325, 352, 597, 617, 747, 765
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 51, 364, 639, 655
HpyF3I CTNAG 4 cut(s) 15, 31, 279, 338
HpySE526I ACGT 3 cut(s) 254, 407, 509
Hsp92I GRCGYC 2 cut(s) 254, 407
Hsp92II CATG 4 cut(s) 25, 49, 122, 829
Ksp22I TGATCA 3 cut(s) 81, 115, 417
Kzo9I GATC 4 cut(s) 81, 103, 115, 417
LmnI GCTCC 2 cut(s) 433, 466
LpnPI CCDG 9 cut(s) 121, 145, 156, 284, 359, 395, 406, 557, 674
Lsp1109I GCAGC 5 cut(s) 73, 175, 364, 382, 609
LweI GCATC 1 cut(s) 604
MaeI CTAG 3 cut(s) 194, 402, 717
MaeII ACGT 3 cut(s) 254, 407, 509
MaeIII GTNAC 1 cut(s) 441
MalI GATC 4 cut(s) 83, 105, 117, 419
MbiI CCGCTC 1 cut(s) 278
MboI GATC 4 cut(s) 81, 103, 115, 417
MboII GAAGA 4 cut(s) 118, 195, 308, 731
MhlI GDGCHC 1 cut(s) 438
MlsI TGGCCA 1 cut(s) 524
MluCI AATT 2 cut(s) 214, 223
MluNI TGGCCA 1 cut(s) 524
MlyI GAGTC 2 cut(s) 407, 629
Mox20I TGGCCA 1 cut(s) 524
MroXI GAANNNNTTC 1 cut(s) 780
MscI TGGCCA 1 cut(s) 524
Msp20I TGGCCA 1 cut(s) 524
MspA1I CMGCKG 1 cut(s) 373
MspI CCGG 2 cut(s) 108, 132
MspR9I CCNGG 2 cut(s) 108, 109
Mva1269I GAATGC 1 cut(s) 312
MwoI GCNNNNNNNGC 5 cut(s) 11, 51, 364, 639, 655
NciI CCSGG 2 cut(s) 108, 109
NdeII GATC 4 cut(s) 81, 103, 115, 417
NlaIII CATG 4 cut(s) 25, 49, 122, 829
NlaIV GGNNCC 2 cut(s) 590, 607
NmuCI GTSAC 1 cut(s) 441
PagI TCATGA 1 cut(s) 118
PctI GAATGC 1 cut(s) 312
PdmI GAANNNNTTC 1 cut(s) 780
PkrI GCNGC 6 cut(s) 63, 165, 369, 372, 379, 599
PleI GAGTC 2 cut(s) 406, 628
PpsI GAGTC 2 cut(s) 406, 628
PpuMI RGGWCCY 1 cut(s) 589
Psp5II RGGWCCY 1 cut(s) 589
PspFI CCCAGC 1 cut(s) 688
PspN4I GGNNCC 2 cut(s) 590, 607
PspPI GGNCC 2 cut(s) 550, 589
PspPPI RGGWCCY 1 cut(s) 589
PvuII CAGCTG 1 cut(s) 373
SatI GCNGC 6 cut(s) 62, 164, 368, 371, 378, 598
Sau3AI GATC 4 cut(s) 81, 103, 115, 417
Sau96I GGNCC 2 cut(s) 550, 589
SchI GAGTC 2 cut(s) 407, 629
ScrFI CCNGG 2 cut(s) 108, 109
SduI GDGCHC 1 cut(s) 438
SfaNI GCATC 1 cut(s) 604
SinI GGWCC 2 cut(s) 550, 589
SmaI CCCGGG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 2 cut(s) 214, 223
SsiI CCGC 4 cut(s) 59, 276, 367, 491
SspMI CTAG 3 cut(s) 194, 402, 717
StyD4I CCNGG 2 cut(s) 106, 107
StyI CCWWGG 1 cut(s) 813
TaiI ACGT 3 cut(s) 257, 410, 512
TaqI TCGA 5 cut(s) 285, 396, 410, 567, 809
TasI AATT 2 cut(s) 214, 223
TauI GCSGC 1 cut(s) 370
TseFI GTSAC 1 cut(s) 441
TseI GCWGC 5 cut(s) 61, 163, 370, 377, 597
Tsp45I GTSAC 1 cut(s) 441
TspDTI ATGAA 2 cut(s) 164, 173
TspGWI ACGGA 2 cut(s) 518, 618
TspMI CCCGGG 1 cut(s) 107
VpaK11BI GGWCC 2 cut(s) 550, 589
XapI RAATTY 1 cut(s) 214
XmaI CCCGGG 1 cut(s) 107
XmnI GAANNNNTTC 1 cut(s) 780
XspI CTAG 3 cut(s) 194, 402, 717
ZraI GACGTC 2 cut(s) 255, 408
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.