Rorug06G0320800

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003008
Physical Location & Seq
Reverse (-)
47245090 .. 47245608
519 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0320800.1

Sequence Viewer

Length: 519 bp
ATGTCTTATTTGATCGGCATGGATAGTGAGAGCATCAAGAAATACTTGTTCAACATAGCCATGAAAAGCAAATGGGAAGAAGTTGTCCAAGCATATAGGCAAAACAACCTGGCACGCAAGGCAAGGATCACCAAGTCAGGTGACACAGCATTGCACGTAGCAGTATCGGACGACCAAGAGGAACTTGTTGAGAAACTAGTAAGGCTGGTTTCCAAAAAGGAGCTGGAACTCCGAAATGAGCGAGGGAACACCCCTCTCCATTTTGCTGCAGCACAGGGGAATTTGAGAATGTGCGAATGCATTGCCAAACATTATCCTTCTTTGGTTGGTCTTCTCAACAAAGATGAAGAGACCCCTCTCTTCTTGGCTGCTCTCCATGGTAAAAAAGATGCCTTCTTGTGTCTCCACTACATTTGTACTCCTGACGAGTCTCCTCCAAACTACATTTATTGTAGGAGGAAGGATGGAGAGACCATATTGCACTGTACAATTTCTGGGGAATACTATGGTAATTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

172

Amino Acids

19.6

Weight (kDa)

6.99

Isoelectric Point (pI)

45.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 65 - 133 1.6e-08 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 134
AcsI RAATTY 1 cut(s) 280
AfaI GTAC 2 cut(s) 418, 487
AgsI TTSAA 1 cut(s) 52
AhlI ACTAGT 1 cut(s) 196
AjnI CCWGG 1 cut(s) 108
AluBI AGCT 1 cut(s) 223
AluI AGCT 1 cut(s) 223
Alw26I GTCTC 4 cut(s) 344, 407, 435, 464
AlwI GGATC 1 cut(s) 134
ApeKI GCWGC 3 cut(s) 266, 269, 368
ApoI RAATTY 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 121, 152
BbsI GAAGAC 1 cut(s) 323
BbvI GCAGC 3 cut(s) 253, 281, 355
BccI CCATC 1 cut(s) 458
BcgI CGANNNNNNTGC 2 cut(s) 284, 318
BciT130I CCWGG 1 cut(s) 110
BcoDI GTCTC 4 cut(s) 344, 407, 435, 464
BcuI ACTAGT 1 cut(s) 196
BfaI CTAG 1 cut(s) 197
BfmI CTRYAG 1 cut(s) 267
BisI GCNGC 3 cut(s) 267, 270, 369
BlsI GCNGC 3 cut(s) 268, 271, 370
Bme1390I CCNGG 1 cut(s) 110
BmrFI CCNGG 1 cut(s) 110
BmsI GCATC 2 cut(s) 42, 379
BpiI GAAGAC 1 cut(s) 323
BsaAI YACGTR 1 cut(s) 157
BsaI GGTCTC 2 cut(s) 344, 464
BsaJI CCNNGG 1 cut(s) 376
Bse3DI GCAATG 2 cut(s) 149, 300
BseBI CCWGG 1 cut(s) 110
BseDI CCNNGG 1 cut(s) 376
BseGI GGATG 1 cut(s) 469
BseMI GCAATG 2 cut(s) 149, 300
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 3 cut(s) 253, 281, 355
BsmAI GTCTC 4 cut(s) 344, 407, 435, 464
BsmI GAATGC 1 cut(s) 302
Bso31I GGTCTC 2 cut(s) 344, 464
Bsp1407I TGTACA 1 cut(s) 485
Bsp143I GATC 2 cut(s) 12, 126
Bsp19I CCATGG 1 cut(s) 376
BspMAI CTGCAG 1 cut(s) 271
BspPI GGATC 1 cut(s) 134
BspTNI GGTCTC 2 cut(s) 344, 464
BsrDI GCAATG 2 cut(s) 149, 300
BsrGI TGTACA 1 cut(s) 485
BssECI CCNNGG 1 cut(s) 376
BssMI GATC 2 cut(s) 12, 126
BssT1I CCWWGG 1 cut(s) 376
Bst2UI CCWGG 1 cut(s) 110
Bst4CI ACNGT 1 cut(s) 485
Bst6I CTCTTC 2 cut(s) 342, 365
BstAUI TGTACA 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 115
BstDSI CCRYGG 1 cut(s) 376
BstF5I GGATG 1 cut(s) 469
BstKTI GATC 2 cut(s) 15, 129
BstMAI GTCTC 4 cut(s) 344, 407, 435, 464
BstMBI GATC 2 cut(s) 12, 126
BstMWI GCNNNNNNNGC 1 cut(s) 119
BstNI CCWGG 1 cut(s) 110
BstSCI CCNGG 1 cut(s) 108
BstSFI CTRYAG 1 cut(s) 267
BstV1I GCAGC 3 cut(s) 253, 281, 355
BstV2I GAAGAC 1 cut(s) 323
BtgI CCRYGG 1 cut(s) 376
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 481
Cac8I GCNNGC 1 cut(s) 115
Csp6I GTAC 2 cut(s) 417, 486
CspCI CAANNNNNGTGG 2 cut(s) 395, 430
CviAII CATG 3 cut(s) 19, 61, 377
CviJI RGCY 4 cut(s) 59, 205, 223, 368
CviKI_1 RGCY 4 cut(s) 59, 205, 223, 368
CviQI GTAC 2 cut(s) 417, 486
DpnI GATC 2 cut(s) 14, 128
DpnII GATC 2 cut(s) 12, 126
Eam1104I CTCTTC 2 cut(s) 342, 365
EarI CTCTTC 2 cut(s) 342, 365
Eco130I CCWWGG 1 cut(s) 376
Eco31I GGTCTC 2 cut(s) 344, 464
EcoRII CCWGG 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 376
EcoT22I ATGCAT 1 cut(s) 302
ErhI CCWWGG 1 cut(s) 376
FaeI CATG 3 cut(s) 22, 64, 380
FaiI YATR 8 cut(s) 20, 56, 62, 94, 96, 378, 476, 507
FalI AAGNNNNNCTT 4 cut(s) 29, 61, 168, 200
FatI CATG 3 cut(s) 18, 60, 376
Fnu4HI GCNGC 3 cut(s) 267, 270, 369
FokI GGATG 1 cut(s) 476
Fsp4HI GCNGC 3 cut(s) 267, 270, 369
FspBI CTAG 1 cut(s) 197
GluI GCNGC 3 cut(s) 267, 270, 369
Hin1II CATG 3 cut(s) 22, 64, 380
HinfI GANTC 1 cut(s) 428
HphI GGTGA 2 cut(s) 121, 152
Hpy188I TCNGA 2 cut(s) 169, 233
Hpy188III TCNNGA 2 cut(s) 37, 422
HpyAV CCTTC 3 cut(s) 327, 403, 454
HpyCH4III ACNGT 1 cut(s) 485
HpyCH4IV ACGT 1 cut(s) 156
HpyCH4V TGCA 4 cut(s) 154, 269, 300, 481
HpyF10VI GCNNNNNNNGC 1 cut(s) 119
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 3 cut(s) 22, 64, 380
Kzo9I GATC 2 cut(s) 12, 126
LmnI GCTCC 1 cut(s) 220
LpnPI CCDG 8 cut(s) 95, 122, 123, 191, 209, 260, 435, 480
Lsp1109I GCAGC 3 cut(s) 253, 281, 355
LweI GCATC 2 cut(s) 42, 379
MaeI CTAG 1 cut(s) 197
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 140
MalI GATC 2 cut(s) 14, 128
MboI GATC 2 cut(s) 12, 126
MboII GAAGA 4 cut(s) 89, 323, 352, 359
MluCI AATT 3 cut(s) 280, 489, 511
MlyI GAGTC 1 cut(s) 437
MnlI CCTC 6 cut(s) 172, 236, 264, 366, 444, 450
Mph1103I ATGCAT 1 cut(s) 302
MseI TTAA 1 cut(s) 517
MslI CAYNNNNRTG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 110
Mva1269I GAATGC 1 cut(s) 302
MvaI CCWGG 1 cut(s) 110
MwoI GCNNNNNNNGC 1 cut(s) 119
NcoI CCATGG 1 cut(s) 376
NdeII GATC 2 cut(s) 12, 126
NlaIII CATG 3 cut(s) 22, 64, 380
NmuCI GTSAC 1 cut(s) 140
NsiI ATGCAT 1 cut(s) 302
PctI GAATGC 1 cut(s) 302
PkrI GCNGC 3 cut(s) 268, 271, 370
PleI GAGTC 1 cut(s) 436
PpsI GAGTC 1 cut(s) 436
Ppu21I YACGTR 1 cut(s) 157
Psp6I CCWGG 1 cut(s) 108
PspGI CCWGG 1 cut(s) 108
PstI CTGCAG 1 cut(s) 271
RsaI GTAC 2 cut(s) 418, 487
RsaNI GTAC 2 cut(s) 417, 486
RseI CAYNNNNRTG 1 cut(s) 59
SaqAI TTAA 1 cut(s) 517
SatI GCNGC 3 cut(s) 267, 270, 369
Sau3AI GATC 2 cut(s) 12, 126
SchI GAGTC 1 cut(s) 437
ScrFI CCNGG 1 cut(s) 110
SetI ASST 4 cut(s) 111, 142, 159, 225
SfaNI GCATC 2 cut(s) 42, 379
SfcI CTRYAG 1 cut(s) 267
SmiMI CAYNNNNRTG 1 cut(s) 59
SpeI ACTAGT 1 cut(s) 196
Sse9I AATT 3 cut(s) 280, 489, 511
SspMI CTAG 1 cut(s) 197
StyD4I CCNGG 1 cut(s) 108
StyI CCWWGG 1 cut(s) 376
TaaI ACNGT 1 cut(s) 485
TaiI ACGT 1 cut(s) 159
TasI AATT 3 cut(s) 280, 489, 511
TatI WGTACW 2 cut(s) 416, 485
Tru1I TTAA 1 cut(s) 517
Tru9I TTAA 1 cut(s) 517
TscAI CASTG 1 cut(s) 488
TseFI GTSAC 1 cut(s) 140
TseI GCWGC 3 cut(s) 266, 269, 368
Tsp45I GTSAC 1 cut(s) 140
TspDTI ATGAA 2 cut(s) 77, 360
TspRI CASTG 1 cut(s) 488
XapI RAATTY 1 cut(s) 280
XcmI CCANNNNNNNNNTGG 1 cut(s) 220
XspI CTAG 1 cut(s) 197
Zsp2I ATGCAT 1 cut(s) 302
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.