Rw6G037670

BOI-related E3 ubiquitin-protein ligase 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0003008
Physical Location & Seq
Forward (+)
61929022 .. 61930321
1300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037670.1

Sequence Viewer

Length: 864 bp
ATGGCAGTTCAAGCTCAGACCATGTATTTCTCAGAAAATTTAGGCATGAGTGGCTTTTCGCAGCAACAGGATTGGGCTCTGATCAACCCTCCTCATCCGCTTTCCGATTCCGGGTTTGATCATGACTTCTTTTCCGGCATCATTCCAAACCGGCCTCAACCTTATCAGCAGCAACAAGCTCCTCAGTTTCATCAAAATCTAGAAGCTAACCGGTTAGGAGTTTCTTCTATTTCTCTTAATTCCACTTGCAATAGCTTCCTTCCCACAACGTCATTATTTCCCGATACTCTGACCGCTCAGCTCGACTTGCAAAGGCGGGAGATAGATTGCATTCTTCAGTTGCAGAATGACAAGCTGAGATTTGCTTTGCAAGAGCAGAGGAAGCGACAGCTGGCGGCTCTGTTGAGCAACTTCGAGTCGAGGACGTCGAGTTTGATGAGGCAAAAAGAGGAGCACTTATCACAAGCGAGAAAGAGAGCAATAGAGCTTCAAGATTGCTTGAGAAAAGCGGAGATGGAGACCGAGACGTGGCAGAGATTGGCCAAAGCAAACGAGACGACGGTGATGGACCTCAACAACGCGCTCGAACAGGTCAAAGAGAGAATGGTCCTGTGCAGCAACGAAGTCGATGATGCAGAGTCCTTCTGTGGCTCGTGTGAGAAGCAGCAGCAACAAGTGCTTTCAGAAAAAGTAACTCACAAAAAGTTGGGTTTGGATTGTAAAAGCTGTAATGCTAGAAGGTCGTGCGTGCTTTTTCTTCCTTGCAGACACCTCTGTTCGTGCAAGTTTTGTGAAGCTTTTCTTGATTGCTGCCCCGTGTGTAAATCCACCAAGGAGGCAAGCATGGAGGTCTTTTTGGTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

287

Amino Acids

32.76

Weight (kDa)

6.11

Isoelectric Point (pI)

57.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 239 - 279 2e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 428
AccBSI CCGCTC 1 cut(s) 296
AccII CGCG 1 cut(s) 581
AciI CCGC 5 cut(s) 98, 294, 316, 395, 509
AcoI YGGCCR 1 cut(s) 540
AcsI RAATTY 1 cut(s) 37
AcuI CTGAAG 1 cut(s) 320
AcyI GRCGYC 1 cut(s) 425
AfiI CCNNNNNNNGG 2 cut(s) 111, 528
AgeI ACCGGT 1 cut(s) 210
AgsI TTSAA 2 cut(s) 11, 491
AjiI CACGTC 1 cut(s) 528
Alw21I GWGCWC 1 cut(s) 456
Alw26I GTCTC 3 cut(s) 512, 518, 548
AoxI GGCC 2 cut(s) 152, 540
ApeKI GCWGC 6 cut(s) 61, 169, 615, 664, 667, 810
ApoI RAATTY 1 cut(s) 37
ArsI GACNNNNNNTTYG 2 cut(s) 415, 447
AsiGI ACCGGT 1 cut(s) 210
Asp700I GAANNNNTTC 1 cut(s) 798
AspLEI GCGC 1 cut(s) 583
AspS9I GGNCC 2 cut(s) 568, 607
AsuC2I CCSGG 1 cut(s) 112
AsuHPI GGTGA 1 cut(s) 574
AvaII GGWCC 2 cut(s) 568, 607
BalI TGGCCA 1 cut(s) 542
BanII GRGCYC 1 cut(s) 79
BauI CACGAG 1 cut(s) 652
Bbv12I GWGCWC 1 cut(s) 456
BbvI GCAGC 6 cut(s) 73, 181, 627, 676, 679, 797
BccI CCATC 2 cut(s) 508, 559
BcgI CGANNNNNNTGC 2 cut(s) 607, 641
BclI TGATCA 2 cut(s) 81, 118
BcnI CCSGG 1 cut(s) 112
BcoDI GTCTC 3 cut(s) 512, 518, 548
BfaI CTAG 2 cut(s) 200, 735
BisI GCNGC 7 cut(s) 62, 170, 396, 616, 665, 668, 811
BlpI GCTNAGC 1 cut(s) 297
BlsI GCNGC 7 cut(s) 63, 171, 397, 617, 666, 669, 812
Bme1390I CCNGG 1 cut(s) 112
Bme18I GGWCC 2 cut(s) 568, 607
BmgBI CACGTC 1 cut(s) 528
BmgT120I GGNCC 2 cut(s) 568, 607
BmrFI CCNGG 1 cut(s) 112
BmsI GCATC 2 cut(s) 147, 622
Bpu1102I GCTNAGC 1 cut(s) 297
BpuEI CTTGAG 1 cut(s) 520
BpuMI CCSGG 1 cut(s) 112
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 512
BsaJI CCNNGG 1 cut(s) 831
BsaWI WCCGGW 1 cut(s) 210
Bsc4I CCNNNNNNNGG 2 cut(s) 111, 528
Bse118I RCCGGY 2 cut(s) 150, 210
BseDI CCNNGG 1 cut(s) 831
BseGI GGATG 1 cut(s) 94
BseLI CCNNNNNNNGG 2 cut(s) 111, 528
BseMII CTCAG 5 cut(s) 29, 45, 197, 311, 347
BseRI GAGGAG 3 cut(s) 81, 171, 464
BseXI GCAGC 6 cut(s) 73, 181, 627, 676, 679, 797
BsgI GTGCAG 1 cut(s) 634
Bsh1236I CGCG 1 cut(s) 581
BshFI GGCC 2 cut(s) 154, 542
BshTI ACCGGT 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 456
BsiSI CCGG 4 cut(s) 111, 135, 151, 211
BslI CCNNNNNNNGG 2 cut(s) 111, 528
BsmAI GTCTC 3 cut(s) 512, 518, 548
BsmBI CGTCTC 2 cut(s) 518, 548
BsmI GAATGC 1 cut(s) 330
BsnI GGCC 2 cut(s) 154, 542
Bso31I GGTCTC 1 cut(s) 512
Bsp1286I GDGCHC 2 cut(s) 79, 456
Bsp143I GATC 2 cut(s) 81, 118
Bsp1720I GCTNAGC 1 cut(s) 297
BspACI CCGC 5 cut(s) 98, 294, 316, 395, 509
BspANI GGCC 2 cut(s) 154, 542
BspCNI CTCAG 5 cut(s) 28, 44, 196, 310, 348
BspFNI CGCG 1 cut(s) 581
BspHI TCATGA 1 cut(s) 121
BspTNI GGTCTC 1 cut(s) 512
BsrBI CCGCTC 1 cut(s) 296
BsrFI RCCGGY 2 cut(s) 150, 210
BssAI RCCGGY 2 cut(s) 150, 210
BssECI CCNNGG 1 cut(s) 831
BssMI GATC 2 cut(s) 81, 118
BssNI GRCGYC 1 cut(s) 425
BssSI CACGAG 1 cut(s) 652
BssT1I CCWWGG 1 cut(s) 831
Bst2BI CACGAG 1 cut(s) 652
Bst4CI ACNGT 1 cut(s) 562
BstACI GRCGYC 1 cut(s) 425
BstAPI GCANNNNNTGC 1 cut(s) 676
BstC8I GCNNGC 3 cut(s) 393, 749, 841
BstDEI CTNAG 5 cut(s) 15, 31, 183, 297, 356
BstF5I GGATG 1 cut(s) 94
BstFNI CGCG 1 cut(s) 581
BstHHI GCGC 1 cut(s) 583
BstKTI GATC 2 cut(s) 84, 121
BstMAI GTCTC 3 cut(s) 512, 518, 548
BstMBI GATC 2 cut(s) 81, 118
BstMWI GCNNNNNNNGC 5 cut(s) 11, 51, 307, 382, 676
BstSCI CCNGG 1 cut(s) 110
BstUI CGCG 1 cut(s) 581
BstV1I GCAGC 6 cut(s) 73, 181, 627, 676, 679, 797
BsuRI GGCC 2 cut(s) 154, 542
BtrI CACGTC 1 cut(s) 528
BtsCI GGATG 1 cut(s) 94
Cac8I GCNNGC 3 cut(s) 393, 749, 841
CciI TCATGA 1 cut(s) 121
CfoI GCGC 1 cut(s) 583
Cfr10I RCCGGY 2 cut(s) 150, 210
Cfr13I GGNCC 2 cut(s) 568, 607
CspAI ACCGGT 1 cut(s) 210
CviAII CATG 4 cut(s) 22, 46, 122, 844
DdeI CTNAG 5 cut(s) 15, 31, 183, 297, 356
DpnI GATC 2 cut(s) 83, 120
DpnII GATC 2 cut(s) 81, 118
EaeI YGGCCR 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 831
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 512
Eco47I GGWCC 2 cut(s) 568, 607
Eco57I CTGAAG 1 cut(s) 320
EcoT14I CCWWGG 1 cut(s) 831
EcoT38I GRGCYC 1 cut(s) 79
ErhI CCWWGG 1 cut(s) 831
Esp3I CGTCTC 2 cut(s) 518, 548
FaeI CATG 4 cut(s) 25, 49, 125, 847
FaiI YATR 4 cut(s) 23, 47, 123, 845
FalI AAGNNNNNCTT 2 cut(s) 786, 818
FatI CATG 4 cut(s) 21, 45, 121, 843
FauI CCCGC 1 cut(s) 309
FbaI TGATCA 2 cut(s) 81, 118
Fnu4HI GCNGC 7 cut(s) 62, 170, 396, 616, 665, 668, 811
FokI GGATG 1 cut(s) 81
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 7 cut(s) 62, 170, 396, 616, 665, 668, 811
FspBI CTAG 2 cut(s) 200, 735
GlaI GCGC 1 cut(s) 582
GluI GCNGC 7 cut(s) 62, 170, 396, 616, 665, 668, 811
HaeIII GGCC 2 cut(s) 154, 542
HapII CCGG 4 cut(s) 111, 135, 151, 211
HhaI GCGC 1 cut(s) 583
Hin1I GRCGYC 1 cut(s) 425
Hin1II CATG 4 cut(s) 25, 49, 125, 847
Hin6I GCGC 1 cut(s) 581
HinP1I GCGC 1 cut(s) 581
HindIII AAGCTT 1 cut(s) 795
HinfI GANTC 3 cut(s) 107, 416, 638
HpaII CCGG 4 cut(s) 111, 135, 151, 211
HphI GGTGA 1 cut(s) 574
Hpy188I TCNGA 6 cut(s) 18, 34, 81, 106, 291, 685
Hpy188III TCNNGA 5 cut(s) 122, 200, 281, 491, 803
Hpy99I CGWCG 2 cut(s) 430, 562
HpyAV CCTTC 3 cut(s) 269, 652, 732
HpyCH4III ACNGT 1 cut(s) 562
HpyCH4IV ACGT 3 cut(s) 269, 425, 527
HpyCH4V TGCA 9 cut(s) 249, 310, 330, 343, 370, 615, 635, 765, 783
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 51, 307, 382, 676
HpyF3I CTNAG 5 cut(s) 15, 31, 183, 297, 356
HpySE526I ACGT 3 cut(s) 269, 425, 527
Hsp92I GRCGYC 1 cut(s) 425
Hsp92II CATG 4 cut(s) 25, 49, 125, 847
HspAI GCGC 1 cut(s) 581
Ksp22I TGATCA 2 cut(s) 81, 118
Kzo9I GATC 2 cut(s) 81, 118
LmnI GCTCC 2 cut(s) 184, 451
LpnPI CCDG 8 cut(s) 53, 124, 148, 164, 224, 377, 575, 623
Lsp1109I GCAGC 6 cut(s) 73, 181, 627, 676, 679, 797
LweI GCATC 2 cut(s) 147, 622
MaeI CTAG 2 cut(s) 200, 735
MaeII ACGT 3 cut(s) 269, 425, 527
MaeIII GTNAC 1 cut(s) 691
MalI GATC 2 cut(s) 83, 120
MbiI CCGCTC 1 cut(s) 296
MboI GATC 2 cut(s) 81, 118
MboII GAAGA 3 cut(s) 216, 326, 749
MhlI GDGCHC 2 cut(s) 79, 456
MlsI TGGCCA 1 cut(s) 542
MluCI AATT 2 cut(s) 37, 238
MluNI TGGCCA 1 cut(s) 542
MlyI GAGTC 2 cut(s) 425, 647
Mox20I TGGCCA 1 cut(s) 542
MroXI GAANNNNTTC 1 cut(s) 798
MscI TGGCCA 1 cut(s) 542
MseI TTAA 1 cut(s) 237
Msp20I TGGCCA 1 cut(s) 542
MspA1I CMGCKG 1 cut(s) 391
MspI CCGG 4 cut(s) 111, 135, 151, 211
MspR9I CCNGG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 330
MvnI CGCG 1 cut(s) 581
MwoI GCNNNNNNNGC 5 cut(s) 11, 51, 307, 382, 676
NciI CCSGG 1 cut(s) 112
NdeII GATC 2 cut(s) 81, 118
NlaIII CATG 4 cut(s) 25, 49, 125, 847
PagI TCATGA 1 cut(s) 121
PcsI WCGNNNNNNNCGW 1 cut(s) 425
PctI GAATGC 1 cut(s) 330
PdmI GAANNNNTTC 1 cut(s) 798
PfeI GAWTC 1 cut(s) 107
PinAI ACCGGT 1 cut(s) 210
PkrI GCNGC 7 cut(s) 63, 171, 397, 617, 666, 669, 812
PleI GAGTC 2 cut(s) 424, 646
PpsI GAGTC 2 cut(s) 424, 646
PspPI GGNCC 2 cut(s) 568, 607
PvuII CAGCTG 1 cut(s) 391
SaqAI TTAA 1 cut(s) 237
SatI GCNGC 7 cut(s) 62, 170, 396, 616, 665, 668, 811
Sau3AI GATC 2 cut(s) 81, 118
Sau96I GGNCC 2 cut(s) 568, 607
SchI GAGTC 2 cut(s) 425, 647
ScrFI CCNGG 1 cut(s) 112
SduI GDGCHC 2 cut(s) 79, 456
SfaNI GCATC 2 cut(s) 147, 622
SinI GGWCC 2 cut(s) 568, 607
SmlI CTYRAG 1 cut(s) 499
SmoI CTYRAG 1 cut(s) 499
Sse9I AATT 2 cut(s) 37, 238
SsiI CCGC 5 cut(s) 98, 294, 316, 395, 509
SspMI CTAG 2 cut(s) 200, 735
StyD4I CCNGG 1 cut(s) 110
StyI CCWWGG 1 cut(s) 831
TaaI ACNGT 1 cut(s) 562
TaiI ACGT 3 cut(s) 272, 428, 530
TaqI TCGA 6 cut(s) 303, 414, 419, 428, 585, 627
TaqII GACCGA 1 cut(s) 536
TasI AATT 2 cut(s) 37, 238
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 1 cut(s) 107
Tru1I TTAA 1 cut(s) 237
Tru9I TTAA 1 cut(s) 237
TseI GCWGC 6 cut(s) 61, 169, 615, 664, 667, 810
TspDTI ATGAA 1 cut(s) 179
VpaK11BI GGWCC 2 cut(s) 568, 607
XapI RAATTY 1 cut(s) 37
XbaI TCTAGA 1 cut(s) 199
XmnI GAANNNNTTC 1 cut(s) 798
XspI CTAG 2 cut(s) 200, 735
ZraI GACGTC 1 cut(s) 426
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.