MD05G1122000.v1.1

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003008
Physical Location & Seq
Forward (+)
24441119 .. 24442589
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1122000.v1.1.491

Sequence Viewer

Length: 855 bp
ATGGCCATTGAGGCTCAGATGTATTCGAAAAATCTTGGGTTTCCATTGTTTGGCTCACAGGATTGGATGGTGGAAAATTGTGGGGGTGGTATTGGTGGGTTCAGTCAGTTTAGTTTCAATGCTCAACAGAAACACCAACTTCAACAACAATTTCAGCAGCAACAACTACAACTACAAAACCAGCAGCAGATAATGGATCAAAATCTCTGCTTTGAAAACAGTATTCCTGTCCCCACTTTGGAAAACAACAGCAGCAACATTATGGCCTCCTCCTTTTCTCAAACATTTGAAGCTCAGGCTGCCAAGCAGAGGCAAGAGATTGATCAGTACATCAGATTACAGAGTGAGAGATTGAGAATGATGCTGCAAGAGCAGAGAAAGCAGCAGCTCGCAATGCTGCTGAAGAATATAGACTCCAAGATACAAGTACTTTTAAAGCAGAAAGACGAAGAAATCACACAAGCAAACAGAAGACAAATGGAGCTCGAAGATTTTCTCAGAAAATTGGAGGCAGAAAACCTGGCATGGCAAAGAGTGGCACAAGAAAACGAAGCCATGGTCATGACATTAAACAACACACTAGAACAGTTCAGGGAGAGGGCCATGTTTTGCAACGTGAATGGTGGAGAAGATGCAGAGTCCTGCTGTGACAATTACAGAGAAGAAGAAGGAGAGAGTGATGGAGGAGGAGGTAAAAAGATGAAGGCTTGCAGAAGATGTAATTCTGGGAGTTCGTGTGTGCTGTTTCTTCCCTGCAGGCACCTCTGTTCATGCAAAGATTGTGAGGCCGTTCTGGATTACTGCCCGGTTTGTAGAACGCCAAAGAAGGCCAGCATTGAGGCTTTGCTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008589 GO:0009966 GO:0009968 GO:0010646 GO:0010648 GO:0023051 GO:0023057 GO:0045879 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

285

Amino Acids

32.59

Weight (kDa)

5.24

Isoelectric Point (pI)

66.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 235 - 276 2.4e-09 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 759
AccB7I CCANNNNNTGG 1 cut(s) 50
AclWI GGATC 1 cut(s) 204
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 422
AfaI GTAC 2 cut(s) 329, 429
AfiI CCNNNNNNNGG 3 cut(s) 50, 238, 309
AgsI TTSAA 4 cut(s) 118, 143, 215, 290
AjnI CCWGG 1 cut(s) 519
AluBI AGCT 3 cut(s) 293, 388, 484
AluI AGCT 3 cut(s) 293, 388, 484
Alw21I GWGCWC 1 cut(s) 486
AlwI GGATC 1 cut(s) 204
AoxI GGCC 5 cut(s) 3, 264, 600, 786, 828
ApeKI GCWGC 8 cut(s) 157, 184, 252, 299, 364, 382, 385, 397
ArsI GACNNNNNNTTYG 2 cut(s) 543, 575
Asp700I GAANNNNTTC 1 cut(s) 492
AspS9I GGNCC 1 cut(s) 600
AsuC2I CCSGG 1 cut(s) 806
AsuII TTCGAA 1 cut(s) 26
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 759
BanII GRGCYC 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 478
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 8 cut(s) 169, 196, 264, 286, 351, 384, 394, 397
BccI CCATC 2 cut(s) 61, 674
BceAI ACGGC 1 cut(s) 773
BciT130I CCWGG 1 cut(s) 521
BclI TGATCA 1 cut(s) 322
BcnI CCSGG 1 cut(s) 806
BfaI CTAG 2 cut(s) 581, 853
BfmI CTRYAG 1 cut(s) 754
BglI GCCNNNNNGGC 1 cut(s) 11
BisI GCNGC 8 cut(s) 158, 185, 253, 300, 365, 383, 386, 398
BlsI GCNGC 8 cut(s) 159, 186, 254, 301, 366, 384, 387, 399
BmcAI AGTACT 1 cut(s) 429
Bme1390I CCNGG 2 cut(s) 521, 806
BmgT120I GGNCC 1 cut(s) 600
BmiI GGNNCC 1 cut(s) 761
BmrFI CCNGG 2 cut(s) 521, 806
BmsI GCATC 2 cut(s) 351, 622
BpiI GAAGAC 1 cut(s) 478
Bpu10I CCTNAGC 1 cut(s) 294
Bpu14I TTCGAA 1 cut(s) 26
BpuMI CCSGG 1 cut(s) 806
BsaBI GATNNNNATC 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 555
BsaXI ACNNNNNCTCC 2 cut(s) 721, 751
Bsc4I CCNNNNNNNGG 3 cut(s) 50, 238, 309
Bse3DI GCAATG 1 cut(s) 399
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 1 cut(s) 521
BseDI CCNNGG 1 cut(s) 555
BseGI GGATG 1 cut(s) 72
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 50, 238, 309
BseMI GCAATG 1 cut(s) 399
BseMII CTCAG 3 cut(s) 29, 308, 511
BseRI GAGGAG 3 cut(s) 259, 699, 702
BseXI GCAGC 8 cut(s) 169, 196, 264, 286, 351, 384, 394, 397
BshFI GGCC 5 cut(s) 5, 266, 602, 788, 830
BshNI GGYRCC 1 cut(s) 759
BsiHKAI GWGCWC 1 cut(s) 486
BsiSI CCGG 1 cut(s) 806
BslFI GGGAC 1 cut(s) 215
BslI CCNNNNNNNGG 3 cut(s) 50, 238, 309
BsmFI GGGAC 1 cut(s) 215
BsnI GGCC 5 cut(s) 5, 266, 602, 788, 830
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 1 cut(s) 486
Bsp143I GATC 2 cut(s) 196, 322
Bsp19I CCATGG 1 cut(s) 555
BspANI GGCC 5 cut(s) 5, 266, 602, 788, 830
BspCNI CTCAG 3 cut(s) 28, 307, 510
BspHI TCATGA 1 cut(s) 561
BspLI GGNNCC 1 cut(s) 761
BspMAI CTGCAG 1 cut(s) 758
BspPI GGATC 1 cut(s) 204
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 759
BsrDI GCAATG 1 cut(s) 399
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 2 cut(s) 196, 322
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 521
Bst4CI ACNGT 2 cut(s) 221, 588
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 4 cut(s) 390, 709, 758, 832
BstDEI CTNAG 3 cut(s) 15, 294, 497
BstDSI CCRYGG 1 cut(s) 555
BstF5I GGATG 1 cut(s) 72
BstKTI GATC 2 cut(s) 199, 325
BstMBI GATC 2 cut(s) 196, 322
BstMWI GCNNNNNNNGC 5 cut(s) 11, 299, 370, 379, 394
BstNI CCWGG 1 cut(s) 521
BstSCI CCNGG 2 cut(s) 519, 804
BstSFI CTRYAG 1 cut(s) 754
BstV1I GCAGC 8 cut(s) 169, 196, 264, 286, 351, 384, 394, 397
BstV2I GAAGAC 1 cut(s) 478
BsuRI GGCC 5 cut(s) 5, 266, 602, 788, 830
BtgI CCRYGG 1 cut(s) 555
BtsCI GGATG 1 cut(s) 72
Cac8I GCNNGC 4 cut(s) 390, 709, 758, 832
CciI TCATGA 1 cut(s) 561
Cfr13I GGNCC 1 cut(s) 600
Csp6I GTAC 2 cut(s) 328, 428
CviAII CATG 5 cut(s) 525, 556, 562, 604, 771
CviQI GTAC 2 cut(s) 328, 428
DdeI CTNAG 3 cut(s) 15, 294, 497
DpnI GATC 2 cut(s) 198, 324
DpnII GATC 2 cut(s) 196, 322
DraI TTTAAA 1 cut(s) 435
EaeI YGGCCR 1 cut(s) 3
Ecl136II GAGCTC 1 cut(s) 484
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 486
Eco53kI GAGCTC 1 cut(s) 484
Eco57I CTGAAG 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 484
EcoRII CCWGG 1 cut(s) 519
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 5 cut(s) 528, 559, 565, 607, 774
FaiI YATR 7 cut(s) 263, 410, 526, 557, 563, 605, 772
FaqI GGGAC 1 cut(s) 215
FatI CATG 5 cut(s) 524, 555, 561, 603, 770
FbaI TGATCA 1 cut(s) 322
Fnu4HI GCNGC 8 cut(s) 158, 185, 253, 300, 365, 383, 386, 398
FokI GGATG 1 cut(s) 79
FriOI GRGCYC 1 cut(s) 486
Fsp4HI GCNGC 8 cut(s) 158, 185, 253, 300, 365, 383, 386, 398
FspBI CTAG 2 cut(s) 581, 853
GluI GCNGC 8 cut(s) 158, 185, 253, 300, 365, 383, 386, 398
HaeIII GGCC 5 cut(s) 5, 266, 602, 788, 830
HapII CCGG 1 cut(s) 806
Hin1II CATG 5 cut(s) 528, 559, 565, 607, 774
HinfI GANTC 2 cut(s) 413, 638
HpaII CCGG 1 cut(s) 806
Hpy188I TCNGA 3 cut(s) 18, 335, 500
Hpy188III TCNNGA 2 cut(s) 562, 794
HpyAV CCTTC 3 cut(s) 662, 697, 820
HpyCH4III ACNGT 2 cut(s) 221, 588
HpyCH4IV ACGT 1 cut(s) 615
HpyCH4V TGCA 6 cut(s) 367, 612, 635, 711, 756, 774
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 299, 370, 379, 394
HpyF3I CTNAG 3 cut(s) 15, 294, 497
HpySE526I ACGT 1 cut(s) 615
Hsp92II CATG 5 cut(s) 528, 559, 565, 607, 774
Ksp22I TGATCA 1 cut(s) 322
Kzo9I GATC 2 cut(s) 196, 322
LmnI GCTCC 1 cut(s) 481
Lsp1109I GCAGC 8 cut(s) 169, 196, 264, 286, 351, 384, 394, 397
LweI GCATC 2 cut(s) 351, 622
MaeI CTAG 2 cut(s) 581, 853
MaeII ACGT 1 cut(s) 615
MaeIII GTNAC 1 cut(s) 647
MalI GATC 2 cut(s) 198, 324
MboI GATC 2 cut(s) 196, 322
MboII GAAGA 9 cut(s) 415, 461, 483, 500, 641, 674, 677, 726, 740
MhlI GDGCHC 1 cut(s) 486
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 5 cut(s) 76, 149, 503, 652, 721
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 407, 647
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 492
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 434, 569
MslI CAYNNNNRTG 1 cut(s) 560
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 806
MspR9I CCNGG 2 cut(s) 521, 806
MvaI CCWGG 1 cut(s) 521
MwoI GCNNNNNNNGC 5 cut(s) 11, 299, 370, 379, 394
NciI CCSGG 1 cut(s) 806
NcoI CCATGG 1 cut(s) 555
NdeII GATC 2 cut(s) 196, 322
NlaIII CATG 5 cut(s) 528, 559, 565, 607, 774
NlaIV GGNNCC 1 cut(s) 761
NmuCI GTSAC 1 cut(s) 647
NspV TTCGAA 1 cut(s) 26
PagI TCATGA 1 cut(s) 561
PdmI GAANNNNTTC 1 cut(s) 492
PflMI CCANNNNNTGG 1 cut(s) 50
PkrI GCNGC 8 cut(s) 159, 186, 254, 301, 366, 384, 387, 399
PleI GAGTC 2 cut(s) 407, 646
PpsI GAGTC 2 cut(s) 407, 646
Psp124BI GAGCTC 1 cut(s) 486
Psp6I CCWGG 1 cut(s) 519
PspGI CCWGG 1 cut(s) 519
PspN4I GGNNCC 1 cut(s) 761
PspPI GGNCC 1 cut(s) 600
PstI CTGCAG 1 cut(s) 758
RsaI GTAC 2 cut(s) 329, 429
RsaNI GTAC 2 cut(s) 328, 428
RseI CAYNNNNRTG 1 cut(s) 560
SacI GAGCTC 1 cut(s) 486
SaqAI TTAA 2 cut(s) 434, 569
SatI GCNGC 8 cut(s) 158, 185, 253, 300, 365, 383, 386, 398
Sau3AI GATC 2 cut(s) 196, 322
Sau96I GGNCC 1 cut(s) 600
SbfI CCTGCAGG 1 cut(s) 758
ScaI AGTACT 1 cut(s) 429
SchI GAGTC 2 cut(s) 407, 647
ScrFI CCNGG 2 cut(s) 521, 806
SdaI CCTGCAGG 1 cut(s) 758
SduI GDGCHC 1 cut(s) 486
SetI ASST 7 cut(s) 295, 390, 486, 522, 618, 694, 765
SfaNI GCATC 2 cut(s) 351, 622
SfcI CTRYAG 1 cut(s) 754
SfuI TTCGAA 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 560
Sse8387I CCTGCAGG 1 cut(s) 758
Sse9I AATT 5 cut(s) 76, 149, 503, 652, 721
SspMI CTAG 2 cut(s) 581, 853
SstI GAGCTC 1 cut(s) 486
StyD4I CCNGG 2 cut(s) 519, 804
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 2 cut(s) 221, 588
TaiI ACGT 1 cut(s) 618
TaqI TCGA 2 cut(s) 26, 486
TasI AATT 5 cut(s) 76, 149, 503, 652, 721
TatI WGTACW 2 cut(s) 327, 427
Tru1I TTAA 2 cut(s) 434, 569
Tru9I TTAA 2 cut(s) 434, 569
TseFI GTSAC 1 cut(s) 647
TseI GCWGC 8 cut(s) 157, 184, 252, 299, 364, 382, 385, 397
Tsp45I GTSAC 1 cut(s) 647
TspDTI ATGAA 2 cut(s) 716, 759
Van91I CCANNNNNTGG 1 cut(s) 50
XmnI GAANNNNTTC 1 cut(s) 492
XspI CTAG 2 cut(s) 581, 853
ZrmI AGTACT 1 cut(s) 429
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.