FvH4_2g01150

BOI-related E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003008
Physical Location & Seq
Reverse (-)
1070523 .. 1072626
2104 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g01150.t2

Sequence Viewer

Length: 849 bp
ATGGCTATTGAAGCACAGCTGTATGCAGAGAATTTTGGGTTTCCGATGTTTGGCTCACAATCACAGCCGCAGGACTTGATGGTGGACAACAATGGCTGTGGTGGTTTTGGTGCTGGGTTTAATCAGTTCTGTTTCAATGCCCAGCAGAAACCGCCGCAGCTACTATACCAGCCACCGCCGATGATTCCTGGTACTTTTGTTCCTGCTCCACACAACAACTACTACATGGCTTATACTCGTCAAACCATTGCAGCTCAGGAAGCCAAGCAAAGGCAAGATATTGATGAATATCTCAGATTACAGAATGAGAGATTGAGGTTGGTGCTACAAGAGCAGAGGAAGCAGCAACTCGCATTGTTGATGAAGAAAGTCGAGTCCAGAACAGAAGTGATAATGAAGCAAAAGGATGAAGAAATAGCACAAGCGCTGAAGAAGAGAATGGAGCTGGAAGGTTTCTTGAGGACACTGGAGGCTGAAAATCAAGCATGGCAAAGAGTGGCTCAGGAGAACGAGGCCATGGTCATGTCCTTAAACAACACTCTGGAACAGTTCAAGGAGAGAGCCGCTCGTGGAGTCATGGAAGACGCAGAGTCTTGCTGTGGTAGTAAACAAGAGGAGGAGGAAGATGAAGAAGATACAGGGGATTACAAAGGAGGCAGAGGTTTGAACAATGGTGTGGATGAAGAGATAAAGATGATGGTTTGCAAAGGCTGCAATTCTGAAAGGTCGTGTGTCTTATTCCTCCCCTGCAGGCATCTTTGTTCATGCAGAGCCTGTGAGGTTGGTTTTGATGCTTGTCCTGTTTGCTCAACGCCAAAGAAGGCTTGCATAGAGGCATTGATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008589 GO:0009966 GO:0009968 GO:0010646 GO:0010648 GO:0023051 GO:0023057 GO:0045879 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4_3

Protein Analysis

283

Amino Acids

32.0

Weight (kDa)

4.98

Isoelectric Point (pI)

65.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-C3HC4_3 PF13920 234 - 275 3.8e-07 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 566
AciI CCGC 5 cut(s) 68, 152, 155, 176, 564
AcsI RAATTY 1 cut(s) 31
AcuI CTGAAG 1 cut(s) 449
AfaI GTAC 1 cut(s) 193
AfeI AGCGCT 1 cut(s) 426
AfiI CCNNNNNNNGG 2 cut(s) 50, 270
AgsI TTSAA 4 cut(s) 11, 136, 553, 667
AhdI GACNNNNNGTC 1 cut(s) 589
AjnI CCWGG 1 cut(s) 187
AluBI AGCT 4 cut(s) 19, 160, 254, 445
AluI AGCT 4 cut(s) 19, 160, 254, 445
AlwNI CAGNNNCTG 1 cut(s) 774
Aor51HI AGCGCT 1 cut(s) 426
AoxI GGCC 1 cut(s) 513
ApeKI GCWGC 4 cut(s) 157, 251, 343, 711
ApoI RAATTY 1 cut(s) 31
AspLEI GCGC 1 cut(s) 427
BaeI ACNNNNGTAYC 2 cut(s) 183, 216
BauI CACGAG 1 cut(s) 567
BbsI GAAGAC 1 cut(s) 588
BbvI GCAGC 4 cut(s) 169, 263, 355, 698
BccI CCATC 2 cut(s) 73, 691
BciT130I CCWGG 1 cut(s) 189
BfmI CTRYAG 1 cut(s) 748
BfoI RGCGCY 1 cut(s) 428
BisI GCNGC 7 cut(s) 68, 155, 158, 252, 344, 564, 712
BlsI GCNGC 7 cut(s) 69, 156, 159, 253, 345, 565, 713
Bme1390I CCNGG 1 cut(s) 189
BmeRI GACNNNNNGTC 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 189
BmsI GCATC 2 cut(s) 763, 781
BpiI GAAGAC 1 cut(s) 588
BplI GAGNNNNNCTC 2 cut(s) 550, 582
BpmI CTGGAG 1 cut(s) 488
Bpu10I CCTNAGC 2 cut(s) 255, 501
BpuEI CTTGAG 1 cut(s) 478
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 516
Bsc4I CCNNNNNNNGG 2 cut(s) 50, 270
Bse1I ACTGG 1 cut(s) 471
Bse3DI GCAATG 1 cut(s) 246
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 1 cut(s) 189
BseDI CCNNGG 1 cut(s) 516
BseGI GGATG 2 cut(s) 412, 685
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 50, 270
BseMI GCAATG 1 cut(s) 246
BseMII CTCAG 3 cut(s) 269, 307, 515
BseNI ACTGG 1 cut(s) 471
BseRI GAGGAG 2 cut(s) 629, 632
BseXI GCAGC 4 cut(s) 169, 263, 355, 698
BseYI CCCAGC 2 cut(s) 113, 141
BshFI GGCC 1 cut(s) 515
BslI CCNNNNNNNGG 2 cut(s) 50, 270
BsnI GGCC 1 cut(s) 515
Bsp19I CCATGG 1 cut(s) 516
BspACI CCGC 5 cut(s) 68, 152, 155, 176, 564
BspANI GGCC 1 cut(s) 515
BspCNI CTCAG 3 cut(s) 268, 306, 514
BspMAI CTGCAG 1 cut(s) 752
BsrBI CCGCTC 1 cut(s) 566
BsrDI GCAATG 1 cut(s) 246
BsrI ACTGG 1 cut(s) 471
BssECI CCNNGG 1 cut(s) 516
BssSI CACGAG 1 cut(s) 567
BssT1I CCWWGG 1 cut(s) 516
Bst2BI CACGAG 1 cut(s) 567
Bst2UI CCWGG 1 cut(s) 189
Bst4CI ACNGT 1 cut(s) 549
Bst6I CTCTTC 2 cut(s) 428, 678
BstAPI GCANNNNNTGC 1 cut(s) 711
BstC8I GCNNGC 2 cut(s) 752, 826
BstDEI CTNAG 3 cut(s) 255, 293, 501
BstDSI CCRYGG 1 cut(s) 516
BstF5I GGATG 2 cut(s) 412, 685
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 1 cut(s) 427
BstMWI GCNNNNNNNGC 6 cut(s) 11, 151, 260, 331, 340, 711
BstNI CCWGG 1 cut(s) 189
BstSCI CCNGG 1 cut(s) 187
BstSFI CTRYAG 1 cut(s) 748
BstV1I GCAGC 4 cut(s) 169, 263, 355, 698
BstV2I GAAGAC 1 cut(s) 588
BsuRI GGCC 1 cut(s) 515
BtgI CCRYGG 1 cut(s) 516
BtsCI GGATG 2 cut(s) 412, 685
BtsIMutI CAGTG 1 cut(s) 464
Cac8I GCNNGC 2 cut(s) 752, 826
CaiI CAGNNNCTG 1 cut(s) 774
CfoI GCGC 1 cut(s) 427
CseI GACGC 1 cut(s) 593
Csp6I GTAC 1 cut(s) 192
CspCI CAANNNNNGTGG 2 cut(s) 79, 114
CviAII CATG 6 cut(s) 226, 486, 517, 523, 577, 765
CviQI GTAC 1 cut(s) 192
DdeI CTNAG 3 cut(s) 255, 293, 501
DriI GACNNNNNGTC 1 cut(s) 589
Eam1104I CTCTTC 2 cut(s) 428, 678
Eam1105I GACNNNNNGTC 1 cut(s) 589
EarI CTCTTC 2 cut(s) 428, 678
Eco130I CCWWGG 1 cut(s) 516
Eco47III AGCGCT 1 cut(s) 426
Eco57I CTGAAG 1 cut(s) 449
EcoRII CCWGG 1 cut(s) 187
EcoT14I CCWWGG 1 cut(s) 516
ErhI CCWWGG 1 cut(s) 516
FaeI CATG 6 cut(s) 229, 489, 520, 526, 580, 768
FatI CATG 6 cut(s) 225, 485, 516, 522, 576, 764
Fnu4HI GCNGC 7 cut(s) 68, 155, 158, 252, 344, 564, 712
FokI GGATG 2 cut(s) 419, 692
Fsp4HI GCNGC 7 cut(s) 68, 155, 158, 252, 344, 564, 712
GlaI GCGC 1 cut(s) 426
GluI GCNGC 7 cut(s) 68, 155, 158, 252, 344, 564, 712
GsaI CCCAGC 2 cut(s) 117, 145
GsuI CTGGAG 1 cut(s) 488
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 1 cut(s) 515
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 427
Hin1II CATG 6 cut(s) 229, 489, 520, 526, 580, 768
Hin6I GCGC 1 cut(s) 425
HinP1I GCGC 1 cut(s) 425
HinfI GANTC 4 cut(s) 184, 374, 573, 590
Hpy166II GTNNAC 2 cut(s) 85, 608
Hpy188I TCNGA 3 cut(s) 45, 296, 721
Hpy188III TCNNGA 5 cut(s) 257, 378, 457, 503, 542
Hpy8I GTNNAC 2 cut(s) 85, 608
HpyAV CCTTC 2 cut(s) 443, 814
HpyCH4III ACNGT 1 cut(s) 549
HpyCH4V TGCA 7 cut(s) 26, 251, 705, 714, 750, 768, 828
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 151, 260, 331, 340, 711
HpyF3I CTNAG 3 cut(s) 255, 293, 501
Hsp92II CATG 6 cut(s) 229, 489, 520, 526, 580, 768
HspAI GCGC 1 cut(s) 425
LmnI GCTCC 2 cut(s) 211, 442
Lsp1109I GCAGC 4 cut(s) 169, 263, 355, 698
LweI GCATC 2 cut(s) 763, 781
MbiI CCGCTC 1 cut(s) 566
MboII GAAGA 9 cut(s) 376, 422, 442, 445, 593, 635, 641, 644, 695
MluCI AATT 2 cut(s) 31, 715
MlyI GAGTC 3 cut(s) 383, 582, 599
MseI TTAA 2 cut(s) 120, 530
MslI CAYNNNNRTG 1 cut(s) 521
MspA1I CMGCKG 1 cut(s) 19
MspR9I CCNGG 1 cut(s) 189
MvaI CCWGG 1 cut(s) 189
MwoI GCNNNNNNNGC 6 cut(s) 11, 151, 260, 331, 340, 711
NcoI CCATGG 1 cut(s) 516
NlaIII CATG 6 cut(s) 229, 489, 520, 526, 580, 768
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 7 cut(s) 69, 156, 159, 253, 345, 565, 713
PleI GAGTC 3 cut(s) 382, 581, 598
PpsI GAGTC 3 cut(s) 382, 581, 598
Psp6I CCWGG 1 cut(s) 187
PspFI CCCAGC 2 cut(s) 113, 141
PspGI CCWGG 1 cut(s) 187
PstI CTGCAG 1 cut(s) 752
PstNI CAGNNNCTG 1 cut(s) 774
PvuII CAGCTG 1 cut(s) 19
RsaI GTAC 1 cut(s) 193
RsaNI GTAC 1 cut(s) 192
RseI CAYNNNNRTG 1 cut(s) 521
SaqAI TTAA 2 cut(s) 120, 530
SatI GCNGC 7 cut(s) 68, 155, 158, 252, 344, 564, 712
SbfI CCTGCAGG 1 cut(s) 752
SchI GAGTC 3 cut(s) 383, 582, 599
ScrFI CCNGG 1 cut(s) 189
SdaI CCTGCAGG 1 cut(s) 752
SetI ASST 9 cut(s) 21, 162, 256, 320, 447, 454, 664, 728, 783
SfaNI GCATC 2 cut(s) 763, 781
SfcI CTRYAG 1 cut(s) 748
SmiMI CAYNNNNRTG 1 cut(s) 521
SmlI CTYRAG 1 cut(s) 457
SmoI CTYRAG 1 cut(s) 457
Sse8387I CCTGCAGG 1 cut(s) 752
Sse9I AATT 2 cut(s) 31, 715
SsiI CCGC 5 cut(s) 68, 152, 155, 176, 564
StyD4I CCNGG 1 cut(s) 187
StyI CCWWGG 1 cut(s) 516
TaaI ACNGT 1 cut(s) 549
TaqI TCGA 1 cut(s) 372
TasI AATT 2 cut(s) 31, 715
TauI GCSGC 3 cut(s) 70, 157, 566
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 2 cut(s) 120, 530
Tru9I TTAA 2 cut(s) 120, 530
TscAI CASTG 1 cut(s) 471
TseI GCWGC 4 cut(s) 157, 251, 343, 711
TspDTI ATGAA 7 cut(s) 300, 377, 410, 423, 642, 696, 753
TspRI CASTG 1 cut(s) 471
XapI RAATTY 1 cut(s) 31
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.