AT5G44180

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002907
Physical Location & Seq
Forward (+)
17782428 .. 17790234
7807 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G44180.2

Sequence Viewer

Length: 5085 bp
ATGGAAGGTGGGTCTGAAAAAACAACACCTGAAGGTTGTGGTGGTGAGAGTAAGTCTAAACGGAAAATGAAAACTGCTGCTCAACTTGAAGTTCTTGAAAACACTTATTCAGCTGAGCCTTATCCTTCGGAAGCTATAAGAGCGGATCTCTCAGTGAAACTGAATCTTTCCGATAGACAGTTACAGATGTGGTTTTGTCACCGGCGGCTTAAAGAACGGAAATCTACTACGCCGAGCAAACGTCAGCGTAAGGAGTTAGTAACTCCAACGGCTATGGAATCCTGGGAACCACCCGTCAATGCCGGTGATTTAGTGGCGGGAAATGAGCTTGATTCTAGAAGAGCTGCTCGAGGCAGTGGCGGTAGTGGTGTGACGGTTGTGAGGCGGTTTAATGAACCGTCTTCTGCTGAGGTTAGAGCTATTGGTTATGTTGAAGCTCAATTGGGAGAGCGGTTGAGAGATAACGGACCGGTTCTTGGAATGGAGTTTGATCCTTTACCTCCTGGTGCATTTGGCATGCCTATTGAGATGCCAAGCCATCGCAAGGCGACTAGGCAAGCTTTCGAGACCAACATATATGTCCGATCCGATGTCAAGCCTATCAAAGATCATGTGAGGCCTATTCGTGAATATCAGTTCATTCCTGAGCTGCCATCTTCGAGGACTGATCATTCGGAACGAGTTTCTCCGTCACATCATTTTGGAGTTCCACTTGATGGTTCGGTTATGAGGGTTTCAGCTGTGTCTGCTGGACATCGGGATGACTATAAAATTTCACCTCAGATACCAAATTTGAATCTTGCAACTCATCAAGGGAAGCCGGGGCATGTCTATTCGCCTAACTTGGTCGAATATGACTCACCGTATCAGAAAAGCTACATGGATACTGCTGCACAAGTTCATGATGATCCTTTTGTGAAATCAGAGAGAGAAGTTGGTAATGAGGATGAGGATGATGATGCCCTGCAATTAGAGAGACACCGCAAGAATGAAGAAGCAAGAATAGCTCGGGAAGTCGAGGCTCATGAGAAGAGGATCCGAAGGGAACTAGAGAAACAAGATATGCTGAGGCGAAAGAGAGAAGAGCAAATAAGGAAAGAAATGGAGAGGCAAGATCGTGAAAGACGGAAAGAGGAAGAACGTCTTTTACGTGAAAAACAGAGAGAGGAAGAGAGGTACCTTAAAGAGCAGATGCGAGAGTTGCAGCGAAGAGAGAAGTTCTTGAAGAAAGAAACAATCAGGGCTGAGAAAATGCGACAAAAAGAAGAGATGCGTAAAGAAAAAGAGGTTGCCAGGCTTAAAGCTGCTAATGAGAGGGCCATTGCTCGTAAGATCGCAAAGGAATCTATGGAACTTATTGAAGATGAACGCTTAGAGCTCATGGAGGTTGCTGCGTTAACAAAAGGCTTGCCTTCAATGCTCGCCCTTGACTTTGAAACTCTACAGAACCTCGATGAATATAGAGACAAGCAGGCAATATTTCCCCCAACATCTGTAAAATTGAAAAAGCCCTTTGCAGTCAAGCCGTGGAATGGTTCTGATGAGAATGTTGCAAATCTTCTTATGGTGTGGAGATTCTTAATCACTTTCGCAGACGTTCTTGGTCTTTGGCCATTTACCCTGGACGAGTTTGCTCAGGCATTCCATGACTATGACCCACGGCTAATGGGAGAGATACACATTGTTCTATTGAAGACTATAATCAAAGATATCGAGGGTGTGGTAAGAACGCTGTCGACCGGTGTTGGAGCAAACCAGAATGTTGCTGCTAATCCCGGAGGGGGTCATCCTCACGTTGTGGAGGGTGCATACGCGTGGGGTTTTGATATACGCAGCTGGAGAAAAAACTTGAATGTTTTTACATGGCCTGAAATCTTGAGGCAACTCGCTCTCTCTGCCGGGTTGGGACCGCAACTGAAGAAAATGAACATTAGGACTGTGTCTGTTCATGATGACAATGAGGCCAACAACTCTGAGAATGTGATTTTCAACCTAAGGAAAGGAGTAGCAGCTGAGAATGCTTTTGCCAAGATGCAAGAGAGGGGACTTTCTAATCCACGACGTTCACGGCATCGTTTGACTCCAGGCACTGTTAAGTTTGCTGCATTTCATGTTCTATCTCTTGAAGGTGAAAAAGGTTTGAACATTCTTGAGGTTGCAGAGAAGATTCAGAAATCAGGATTGAGGGATCTAACGACGAGTAGGACACCTGAAGCCTCGGTTGCTGCTGCGTTGTCTCGAGATACAAAACTCTTTGAGAGGGTAGCTCCTTCTACGTATTGTGTACGTGCTTCCTATAGAAAAGATGCAGGTGATGCTGAGACTATATTTGCTGAAGCGAGAGAGAGAATACGTGCATTCAAGAGCGGCATCACTGATGTAGAAGATGTTGATGACGCTGAGAGAGATGAAGACTCTGAAAGCGATGTCGGAGAAGACCCAGAGGTTGATGTGAACCTTAAAAAGGAAGATCCCAATCCTCTGAAGGTAGAAAACTTAATTGGAGTCGAACCATTGTTGGAAAATGGGAAACTGGATACCGTGCCCATGAAAACTGAACTGGGACTGCCTCTTACTCCCTCTCTCCCCGAGGAAATGAAAGATGAGAAAAGAGATGACACTTTAGCGGACCAATCTCTAGAGGATGCGGTAGCAAACGGTGAAGATAGTGCTTGTTTTGACGAGAGCAAACTTGGGGAACAGTGGGTTCAAGGACTTGTAGAAGGAGATTACTCGAATCTTAGCAGCGAGGAACGTTTAAACGCACTTGTTGCTCTGATTGGTATTGCCACCGAAGGAAACACAATCCGAATCGCCCTTGAGGAACGGTTGGAGGTAGCAAGTGCATTAAAGAAACAGATGTGGGGTGAAGTGCAACTCGACAAACGCTGGAAAGAAGAGTCTTTGATTCGAGCAAATTACCTCTCATACCCAACAGCAAAGCCGGGGCTTAATATCGCAACCCCTGCATCTGGAAATCAAGAAAGTTCATCAGCGGATGTGACTCCAATCTCCTCACAGGACCCGGTGAGTCTTCCACAGATCGATGTGAATAACGTGATTGCTGGACCAAGCTTGCAATTGCAAGAAAATGTACCTGGAGTGGAGAATTTGCAGTATCAGCAGCAACAGGGGTACACAGCGGACCGGGAAAGGCTGCGTGCACAGTTAAAAGCGTATGTTGGGTATAAAGCTGAAGAGCTATATGTATATAGGTCGCTTCCGCTAGGTCAAGATCGAAGACGCAATCGCTATTGGCGGTTTTCAGCATCCGCCTCTCGAAATGATCCTGGTTGTGGTAGAATATTCGTGGAACTTCAGGACGGACGCTGGAGGCTCATTGATTCCGAAGAGGCTTTTGACTATTTGGTGAAGTCACTAGATGTTCGCGGTGTAAGAGAATCACATTTACACTTTATGTTGCTGAAGATCGAAGCATCCTTCAAGGAAGCATTAAGGAGGAATGTGGCAGCAAATCCCGGGGTGTGTTCCATATCTTCTAGCTTGGATTCCGATACTGCGGAAATCTCCACGACGTTTAAGATCGAGCTAGGGGATAGTAACGCCGTTGAGAGATGCAGCGTATTGCAACGCTTCCATAGTTTCGAGAAGTGGATGTGGGATAATATGCTGCATCCTAGTGCTTTATCTGCATTTAAGTATGGTGCTAAGCAAAGCAGTCCGCTTTTTCGCATATGCAGAATCTGTGCGGAACTACACTTTGTCGGAGATATTTGCTGTCCTAGTTGCGGTCAGATGCATGCTGGTCCAGATGTTGGTGAGTTGTGTTTTGCTGAGCAAGTGGCTCAACTCGGGGATAATTTGAGAAGAGGAGATACTGGCTTTATCTTGCGTAGCTCAATCTTGTCTCCTCTTAGAATAAGACTACTCAAGGTTCAGTTAGCACTTGTCGAAGCTTCTCTTCCACCTGAAGGACTTGAAGCTTTTTGGACAGAGAATCTTAGGAAATCTTGGGGTATGAAGCTGTTGTCATCAAGTTCCCATGAAGATCTTTATCAGGTTCTCACAACGTTAGAGGCAGCACTAAAGAGGGATTTCTTGTCTTCAAACTTTGAAACAACTTCTGAACTCTTGGGTTTACAAGAAGGAGCTCTCGCCAGCGATCTTACTTGTGGGGTTAATGTACTACCGTGGATACCAAAGACAGCAGGAGGTGTAGCTTTGAGACTCTTTGATTTCGACAGCTCGATTGTTTACACACCCGATCAAAACAACGATCCTCTAAAAGACAAAGAATCTGAAGATTTCGTGGGTTTGGAGACAAATATTCTGAGAAACTTACATGAGAAGGACGTAATGGAAACTCCGGTGCAAGTTGCTGCTTATAAGCAAGAAGAGAACTGGACGGATCCTGGTTTAGGTGGTGTGTCTAGCTCTGGGAGAGGAGGTCGACCACCACGAGGACGTGGCCGGCCTCGTGCCCGTGGCAATGGCAAGAAACCGGCAGTTTCTGTTAAACCACCACGAGGTGCAGCAAACTCAAATGGTGAAACCATGTTGAGACCGAGAGCACAACCACGTGGGGGTAGAAAGAATGGGCGGCGTAGTGGCACCAAAGGCCGAAAGAGACCAACGCAAGGAACACTCGGTATATGTAATGAAGTAGGAGGAGGACGTCGGGTTAAGGAAGTTGCTGTAACCGCCAAAACCAGTCTTCCTGATAACGATGACGATTGGATCGAAACCCCTGAACTGCAAGACGATGACGGAGAAGCTAGCAGCTCGGGGAGATCGTTTCAGTATGAGGACTATGATGATGATGATGTCATGGCTCCGATTGATGATTTTGATGGCGGCGGCGAATCAAGTAAATTAGTAGGTAGGGGGGAATTTAGCTTACATAGTGATGATGAATACGAAGAAGAAGAGGAAGAAGAAGAAGACATGAACATGAAAATGGATGTGAATGTTGTTGATGATGAGGATGAAGATTACATAAACGAAGACTCGTATGGTAGAAAGCAGCATGGGATTAGCATTAGCAACGATGCAGCGACTCGGAAAAGGTTTAATAAGTTTGAAGATCCTGATCTAACATCTTCATCGTCTTCTGATTTTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0040034 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048506 GO:0048509 GO:0048510 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050793 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1694

Amino Acids

190.38

Weight (kDa)

5.33

Isoelectric Point (pI)

47.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 18 - 74 1.4e-16 Homeodomain
DDT PF02791 515 - 570 1e-19 DDT domain
HARE-HTH PF05066 696 - 764 2e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 899 - 940 1.2e-06 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1071 - 1118 2.8e-10 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4347
AarI CACCTGC 1 cut(s) 2300
AatII GACGTC 1 cut(s) 4639
Acc36I ACCTGC 1 cut(s) 2300
Acc65I GGTACC 1 cut(s) 1176
AccB1I GGYRCC 2 cut(s) 1176, 4571
AccB7I CCANNNNNTGG 2 cut(s) 716, 3776
AccBSI CCGCTC 3 cut(s) 143, 451, 2367
AccI GTMKAC 2 cut(s) 1736, 4411
AccII CGCG 2 cut(s) 1814, 3390
AclI AACGTT 2 cut(s) 2755, 4031
AcoI YGGCCR 2 cut(s) 1610, 4429
AcsI RAATTY 4 cut(s) 771, 790, 3109, 4850
AcvI CACGTG 1 cut(s) 4541
AcyI GRCGYC 1 cut(s) 4636
AdeI CACNNNGTG 2 cut(s) 1798, 4490
AfaI GTAC 5 cut(s) 1178, 2286, 3096, 3137, 4146
AflIII ACRYGT 1 cut(s) 1812
AgeI ACCGGT 2 cut(s) 469, 1739
AjiI CACGTC 1 cut(s) 4427
AjnI CCWGG 8 cut(s) 281, 502, 1292, 1620, 2083, 3097, 3289, 4372
AjuI GAANNNNNNNTTGG 2 cut(s) 2484, 2516
Alw21I GWGCWC 4 cut(s) 1380, 3166, 4114, 4534
Alw44I GTGCAC 1 cut(s) 3162
AlwNI CAGNNNCTG 3 cut(s) 2090, 3705, 4472
Ama87I CYCGRG 7 cut(s) 348, 1008, 2238, 2588, 3479, 3812, 4744
AoxI GGCC 8 cut(s) 617, 1317, 1610, 1865, 1962, 4429, 4433, 4579
ApaLI GTGCAC 1 cut(s) 3162
ApoI RAATTY 4 cut(s) 771, 790, 3109, 4850
ArsI GACNNNNNNTTYG 2 cut(s) 655, 687
AsiGI ACCGGT 2 cut(s) 469, 1739
Asp718I GGTACC 1 cut(s) 1176
AspS9I GGNCC 8 cut(s) 467, 1317, 1907, 2629, 3022, 3068, 3145, 3767
AsuC2I CCSGG 8 cut(s) 822, 1776, 1900, 2946, 3026, 3149, 3480, 3481
AsuNHI GCTAGC 1 cut(s) 4736
AvaI CYCGRG 7 cut(s) 348, 1008, 2238, 2588, 3479, 3812, 4744
AvaII GGWCC 7 cut(s) 467, 1907, 2629, 3022, 3068, 3145, 3767
AxyI CCTNAGG 1 cut(s) 1994
BaeGI GKGCMC 3 cut(s) 2547, 3166, 4444
BalI TGGCCA 1 cut(s) 1612
BamHI GGATCC 2 cut(s) 1035, 4369
BanI GGYRCC 2 cut(s) 1176, 4571
BanII GRGCYC 2 cut(s) 1380, 4114
BarI GAAGNNNNNNTAC 2 cut(s) 3820, 3852
BauI CACGAG 3 cut(s) 4419, 4437, 4485
BbrPI CACGTG 1 cut(s) 4541
Bbv12I GWGCWC 4 cut(s) 1380, 3166, 4114, 4534
BbvCI CCTCAGC 2 cut(s) 408, 1067
BccI CCATC 4 cut(s) 546, 661, 710, 4805
BceAI ACGGC 5 cut(s) 285, 1510, 1676, 2084, 3551
BciT130I CCWGG 8 cut(s) 283, 504, 1294, 1622, 2085, 3099, 3291, 4374
BciVI GTATCC 3 cut(s) 877, 2530, 4149
BclI TGATCA 1 cut(s) 667
BcnI CCSGG 8 cut(s) 822, 1776, 1900, 2946, 3026, 3149, 3480, 3481
BfmI CTRYAG 2 cut(s) 1442, 2296
BfuAI ACCTGC 1 cut(s) 2300
BfuI GTATCC 3 cut(s) 877, 2530, 4149
BglII AGATCT 1 cut(s) 4009
BlpI GCTNAGC 3 cut(s) 114, 3669, 3795
Bme18I GGWCC 7 cut(s) 467, 1907, 2629, 3022, 3068, 3145, 3767
BmeT110I CYCGRG 7 cut(s) 348, 1008, 2238, 2588, 3479, 3812, 4744
BmgBI CACGTC 1 cut(s) 4427
BmgT120I GGNCC 8 cut(s) 467, 1317, 1907, 2629, 3022, 3068, 3145, 3767
BmiI GGNNCC 8 cut(s) 288, 1037, 1178, 1908, 3024, 4371, 4573, 4794
BmrI ACTGGG 1 cut(s) 2570
BmtI GCTAGC 1 cut(s) 4740
BmuI ACTGGG 1 cut(s) 2570
BplI GAGNNNNNCTC 4 cut(s) 132, 164, 2251, 2283
BpmI CTGGAG 4 cut(s) 1858, 2067, 3120, 3352
Bpu10I CCTNAGC 4 cut(s) 408, 645, 1067, 1635
Bpu1102I GCTNAGC 3 cut(s) 114, 3669, 3795
BpuEI CTTGAG 4 cut(s) 1897, 2171, 2840, 3875
BpuMI CCSGG 8 cut(s) 822, 1776, 1900, 2946, 3026, 3149, 3480, 3481
Bsa29I ATCGAT 1 cut(s) 3045
BsaAI YACGTR 5 cut(s) 1151, 2277, 2288, 2354, 4541
BsaBI GATNNNNATC 3 cut(s) 2475, 4014, 5049
BsaHI GRCGYC 1 cut(s) 4636
BsaI GGTCTC 3 cut(s) 560, 4516, 4582
BsaWI WCCGGW 3 cut(s) 469, 1739, 4327
BsaXI ACNNNNNCTCC 2 cut(s) 4393, 4423
Bse118I RCCGGY 6 cut(s) 201, 302, 469, 1739, 4431, 4462
Bse1I ACTGG 5 cut(s) 2538, 2565, 3844, 4367, 4671
Bse21I CCTNAGG 1 cut(s) 1994
Bse3DI GCAATG 2 cut(s) 1320, 4456
Bse8I GATNNNNATC 3 cut(s) 2475, 4014, 5049
BseBI CCWGG 8 cut(s) 283, 504, 1294, 1622, 2085, 3099, 3291, 4374
BseCI ATCGAT 1 cut(s) 3045
BseJI GATNNNNATC 3 cut(s) 2475, 4014, 5049
BseMI GCAATG 2 cut(s) 1320, 4456
BseNI ACTGG 5 cut(s) 2538, 2565, 3844, 4367, 4671
BseRI GAGGAG 5 cut(s) 3004, 3846, 3861, 4419, 4644
BseSI GKGCMC 3 cut(s) 2547, 3166, 4444
BsgI GTGCAG 2 cut(s) 876, 4512
Bsh1236I CGCG 2 cut(s) 1814, 3390
Bsh1285I CGRYCG 1 cut(s) 1740
BshFI GGCC 8 cut(s) 619, 1319, 1612, 1867, 1964, 4431, 4435, 4581
BshNI GGYRCC 2 cut(s) 1176, 4571
BshTI ACCGGT 2 cut(s) 469, 1739
BshVI ATCGAT 1 cut(s) 3045
BsiEI CGRYCG 1 cut(s) 1740
BsiHKAI GWGCWC 4 cut(s) 1380, 3166, 4114, 4534
BsiHKCI CYCGRG 7 cut(s) 348, 1008, 2238, 2588, 3479, 3812, 4744
BslFI GGGAC 3 cut(s) 1920, 2058, 2577
BsmFI GGGAC 3 cut(s) 1920, 2058, 2577
BsmI GAATGC 3 cut(s) 1640, 2023, 2357
BsnI GGCC 8 cut(s) 619, 1319, 1612, 1867, 1964, 4431, 4435, 4581
Bso31I GGTCTC 3 cut(s) 560, 4516, 4582
BsoBI CYCGRG 7 cut(s) 348, 1008, 2238, 2588, 3479, 3812, 4744
Bsp1286I GDGCHC 6 cut(s) 1380, 2547, 3166, 4114, 4444, 4534
Bsp1720I GCTNAGC 3 cut(s) 114, 3669, 3795
BspANI GGCC 8 cut(s) 619, 1319, 1612, 1867, 1964, 4431, 4435, 4581
BspDI ATCGAT 1 cut(s) 3045
BspFNI CGCG 2 cut(s) 1814, 3390
BspHI TCATGA 3 cut(s) 901, 1024, 1948
BspLI GGNNCC 8 cut(s) 288, 1037, 1178, 1908, 3024, 4371, 4573, 4794
BspMI ACCTGC 1 cut(s) 2300
BspOI GCTAGC 1 cut(s) 4740
BspQI GCTCTTC 3 cut(s) 334, 1077, 3192
BspT107I GGYRCC 2 cut(s) 1176, 4571
BspTNI GGTCTC 3 cut(s) 560, 4516, 4582
BsrBI CCGCTC 3 cut(s) 143, 451, 2367
BsrDI GCAATG 2 cut(s) 1320, 4456
BsrFI RCCGGY 6 cut(s) 201, 302, 469, 1739, 4431, 4462
BsrI ACTGG 5 cut(s) 2538, 2565, 3844, 4367, 4671
BssAI RCCGGY 6 cut(s) 201, 302, 469, 1739, 4431, 4462
BssNI GRCGYC 1 cut(s) 4636
BssSI CACGAG 3 cut(s) 4419, 4437, 4485
Bst2BI CACGAG 3 cut(s) 4419, 4437, 4485
Bst2UI CCWGG 8 cut(s) 283, 504, 1294, 1622, 2085, 3099, 3291, 4374
BstACI GRCGYC 1 cut(s) 4636
BstAPI GCANNNNNTGC 3 cut(s) 2315, 2771, 2966
BstBAI YACGTR 5 cut(s) 1151, 2277, 2288, 2354, 4541
BstDSI CCRYGG 4 cut(s) 1526, 1658, 4151, 4444
BstENI CCTNNNNNAGG 2 cut(s) 2462, 4377
BstFNI CGCG 2 cut(s) 1814, 3390
BstMCI CGRYCG 1 cut(s) 1740
BstNI CCWGG 8 cut(s) 283, 504, 1294, 1622, 2085, 3099, 3291, 4374
BstNSI RCATGY 3 cut(s) 520, 830, 3764
BstSFI CTRYAG 2 cut(s) 1442, 2296
BstSLI GKGCMC 3 cut(s) 2547, 3166, 4444
BstSNI TACGTA 1 cut(s) 2277
BstUI CGCG 2 cut(s) 1814, 3390
BstX2I RGATCY 7 cut(s) 145, 1035, 2188, 2470, 4009, 4369, 5044
BstYI RGATCY 7 cut(s) 145, 1035, 2188, 2470, 4009, 4369, 5044
Bsu15I ATCGAT 1 cut(s) 3045
Bsu36I CCTNAGG 1 cut(s) 1994
BsuI GTATCC 3 cut(s) 877, 2530, 4149
BsuRI GGCC 8 cut(s) 619, 1319, 1612, 1867, 1964, 4431, 4435, 4581
BsuTUI ATCGAT 1 cut(s) 3045
BtgI CCRYGG 4 cut(s) 1526, 1658, 4151, 4444
BtgZI GCGATG 2 cut(s) 524, 2439
BtrI CACGTC 1 cut(s) 4427
BtsI GCAGTG 1 cut(s) 361
BtsIMutI CAGTG 5 cut(s) 159, 361, 2088, 2373, 2708
BveI ACCTGC 1 cut(s) 2300
CaiI CAGNNNCTG 3 cut(s) 2090, 3705, 4472
CciI TCATGA 3 cut(s) 901, 1024, 1948
Cfr10I RCCGGY 6 cut(s) 201, 302, 469, 1739, 4431, 4462
Cfr13I GGNCC 8 cut(s) 467, 1317, 1907, 2629, 3022, 3068, 3145, 3767
Cfr9I CCCGGG 1 cut(s) 3479
ClaI ATCGAT 1 cut(s) 3045
CpoI CGGWCCG 2 cut(s) 467, 3145
CseI GACGC 3 cut(s) 2405, 3252, 3336
Csp6I GTAC 5 cut(s) 1177, 2285, 3095, 3136, 4145
CspAI ACCGGT 2 cut(s) 469, 1739
CspI CGGWCCG 2 cut(s) 467, 3145
CviQI GTAC 5 cut(s) 1177, 2285, 3095, 3136, 4145
DraI TTTAAA 1 cut(s) 2760
DraIII CACNNNGTG 2 cut(s) 1798, 4490
EaeI YGGCCR 2 cut(s) 1610, 4429
EciI GGCGGA 1 cut(s) 3262
Ecl136II GAGCTC 2 cut(s) 1378, 4112
Eco105I TACGTA 1 cut(s) 2277
Eco147I AGGCCT 1 cut(s) 619
Eco24I GRGCYC 2 cut(s) 1380, 4114
Eco31I GGTCTC 3 cut(s) 560, 4516, 4582
Eco32I GATATC 1 cut(s) 1711
Eco47I GGWCC 7 cut(s) 467, 1907, 2629, 3022, 3068, 3145, 3767
Eco53kI GAGCTC 2 cut(s) 1378, 4112
Eco72I CACGTG 1 cut(s) 4541
Eco81I CCTNAGG 1 cut(s) 1994
Eco88I CYCGRG 7 cut(s) 348, 1008, 2238, 2588, 3479, 3812, 4744
EcoICRI GAGCTC 2 cut(s) 1378, 4112
EcoNI CCTNNNNNAGG 2 cut(s) 2462, 4377
EcoO109I RGGNCCY 1 cut(s) 3022
EcoRII CCWGG 8 cut(s) 281, 502, 1292, 1620, 2083, 3097, 3289, 4372
EcoRV GATATC 1 cut(s) 1711
EcoT22I ATGCAT 1 cut(s) 3762
EcoT38I GRGCYC 2 cut(s) 1380, 4114
FalI AAGNNNNNCTT 4 cut(s) 1128, 1160, 3906, 3938
FaqI GGGAC 3 cut(s) 1920, 2058, 2577
FauI CCCGC 1 cut(s) 310
FauNDI CATATG 1 cut(s) 3695
FbaI TGATCA 1 cut(s) 667
FblI GTMKAC 2 cut(s) 1736, 4411
FriOI GRGCYC 2 cut(s) 1380, 4114
FseI GGCCGGCC 1 cut(s) 4435
GsuI CTGGAG 4 cut(s) 1858, 2067, 3120, 3352
HaeIII GGCC 8 cut(s) 619, 1319, 1612, 1867, 1964, 4431, 4435, 4581
HgaI GACGC 3 cut(s) 2405, 3252, 3336
Hin1I GRCGYC 1 cut(s) 4636
HincII GTYRAC 3 cut(s) 1398, 1737, 4412
HindII GTYRAC 3 cut(s) 1398, 1737, 4412
HindIII AAGCTT 4 cut(s) 558, 3073, 3915, 3942
HpaI GTTAAC 1 cut(s) 1398
Hpy99I CGWCG 4 cut(s) 2064, 2200, 3538, 4641
Hsp92I GRCGYC 1 cut(s) 4636
KpnI GGTACC 1 cut(s) 1180
KroI GCCGGC 1 cut(s) 4431
KroNI GCCGGC 1 cut(s) 4433
Ksp22I TGATCA 1 cut(s) 667
KspAI GTTAAC 1 cut(s) 1398
LguI GCTCTTC 3 cut(s) 334, 1077, 3192
LmnI GCTCC 4 cut(s) 1748, 2272, 4109, 4798
MaeIII GTNAC 9 cut(s) 180, 197, 259, 370, 690, 3001, 3375, 3560, 4657
MbiI CCGCTC 3 cut(s) 143, 451, 2367
MfeI CAATTG 2 cut(s) 440, 3080
MflI RGATCY 7 cut(s) 145, 1035, 2188, 2470, 4009, 4369, 5044
MhlI GDGCHC 6 cut(s) 1380, 2547, 3166, 4114, 4444, 4534
MlsI TGGCCA 1 cut(s) 1612
MluI ACGCGT 1 cut(s) 1812
MluNI TGGCCA 1 cut(s) 1612
MmeI TCCRAC 6 cut(s) 290, 1726, 2410, 2499, 2811, 3706
Mox20I TGGCCA 1 cut(s) 1612
Mph1103I ATGCAT 1 cut(s) 3762
MroNI GCCGGC 1 cut(s) 4431
MscI TGGCCA 1 cut(s) 1612
MslI CAYNNNNRTG 7 cut(s) 759, 1650, 1813, 3639, 4866, 4910, 4916
Msp20I TGGCCA 1 cut(s) 1612
MspA1I CMGCKG 6 cut(s) 113, 740, 1836, 2012, 2996, 3143
MssI GTTTAAAC 1 cut(s) 2760
MunI CAATTG 2 cut(s) 440, 3080
Mva1269I GAATGC 3 cut(s) 1640, 2023, 2357
MvaI CCWGG 8 cut(s) 283, 504, 1294, 1622, 2085, 3099, 3291, 4374
MvnI CGCG 2 cut(s) 1814, 3390
NaeI GCCGGC 1 cut(s) 4433
NciI CCSGG 8 cut(s) 822, 1776, 1900, 2946, 3026, 3149, 3480, 3481
NdeI CATATG 1 cut(s) 3695
NgoMIV GCCGGC 1 cut(s) 4431
NheI GCTAGC 1 cut(s) 4736
NlaIV GGNNCC 8 cut(s) 288, 1037, 1178, 1908, 3024, 4371, 4573, 4794
NmeAIII GCCGAG 1 cut(s) 258
NmuCI GTSAC 5 cut(s) 197, 370, 690, 3001, 3375
NsiI ATGCAT 1 cut(s) 3762
NspI RCATGY 3 cut(s) 520, 830, 3764
PaeI GCATGC 2 cut(s) 520, 3764
PaeR7I CTCGAG 2 cut(s) 348, 2238
PagI TCATGA 3 cut(s) 901, 1024, 1948
PaqCI CACCTGC 1 cut(s) 2300
PceI AGGCCT 1 cut(s) 619
PciSI GCTCTTC 3 cut(s) 334, 1077, 3192
PcsI WCGNNNNNNNCGW 4 cut(s) 1147, 4417, 4698, 4751
PctI GAATGC 3 cut(s) 1640, 2023, 2357
PdiI GCCGGC 1 cut(s) 4433
PflFI GACNNNGTC 1 cut(s) 1939
PflMI CCANNNNNTGG 2 cut(s) 716, 3776
PfoI TCCNGGA 1 cut(s) 1774
PinAI ACCGGT 2 cut(s) 469, 1739
PmaCI CACGTG 1 cut(s) 4541
PmeI GTTTAAAC 1 cut(s) 2760
PmlI CACGTG 1 cut(s) 4541
Ppu21I YACGTR 5 cut(s) 1151, 2277, 2288, 2354, 4541
PpuMI RGGWCCY 1 cut(s) 3022
PsiI TTATAA 1 cut(s) 4347
Psp124BI GAGCTC 2 cut(s) 1380, 4114
Psp1406I AACGTT 2 cut(s) 2755, 4031
Psp5II RGGWCCY 1 cut(s) 3022
Psp6I CCWGG 8 cut(s) 281, 502, 1292, 1620, 2083, 3097, 3289, 4372
PspCI CACGTG 1 cut(s) 4541
PspGI CCWGG 8 cut(s) 281, 502, 1292, 1620, 2083, 3097, 3289, 4372
PspN4I GGNNCC 8 cut(s) 288, 1037, 1178, 1908, 3024, 4371, 4573, 4794
PspPI GGNCC 8 cut(s) 467, 1317, 1907, 2629, 3022, 3068, 3145, 3767
PspPPI RGGWCCY 1 cut(s) 3022
PspXI VCTCGAGB 1 cut(s) 348
PsrI GAACNNNNNNTAC 2 cut(s) 1131, 1163
PstNI CAGNNNCTG 3 cut(s) 2090, 3705, 4472
PsuI RGATCY 7 cut(s) 145, 1035, 2188, 2470, 4009, 4369, 5044
PsyI GACNNNGTC 1 cut(s) 1939
PvuII CAGCTG 4 cut(s) 113, 740, 1836, 2012
RigI GGCCGGCC 1 cut(s) 4435
RsaI GTAC 5 cut(s) 1178, 2286, 3096, 3137, 4146
RsaNI GTAC 5 cut(s) 1177, 2285, 3095, 3136, 4145
RseI CAYNNNNRTG 7 cut(s) 759, 1650, 1813, 3639, 4866, 4910, 4916
Rsr2I CGGWCCG 2 cut(s) 467, 3145
RsrII CGGWCCG 2 cut(s) 467, 3145
SacI GAGCTC 2 cut(s) 1380, 4114
SalI GTCGAC 2 cut(s) 1735, 4410
SapI GCTCTTC 3 cut(s) 334, 1077, 3192
Sau96I GGNCC 8 cut(s) 467, 1317, 1907, 2629, 3022, 3068, 3145, 3767
SduI GDGCHC 6 cut(s) 1380, 2547, 3166, 4114, 4444, 4534
SfcI CTRYAG 2 cut(s) 1442, 2296
Sfr274I CTCGAG 2 cut(s) 348, 2238
SgrAI CRCCGGYG 1 cut(s) 201
SinI GGWCC 7 cut(s) 467, 1907, 2629, 3022, 3068, 3145, 3767
SlaI CTCGAG 2 cut(s) 348, 2238
SmaI CCCGGG 1 cut(s) 3481
SmiMI CAYNNNNRTG 7 cut(s) 759, 1650, 1813, 3639, 4866, 4910, 4916
SmlI CTYRAG 6 cut(s) 348, 1876, 2150, 2238, 2819, 3890
SmoI CTYRAG 6 cut(s) 348, 1876, 2150, 2238, 2819, 3890
SnaBI TACGTA 1 cut(s) 2277
SphI GCATGC 2 cut(s) 520, 3764
SseBI AGGCCT 1 cut(s) 619
SspI AATATT 3 cut(s) 1479, 3306, 4288
SstI GAGCTC 2 cut(s) 1380, 4114
StuI AGGCCT 1 cut(s) 619
TaqII GACCGA 1 cut(s) 4540
TatI WGTACW 1 cut(s) 4144
TauI GCSGC 5 cut(s) 208, 2370, 4564, 4818, 4821
TscAI CASTG 5 cut(s) 159, 361, 2095, 2380, 2708
TseFI GTSAC 5 cut(s) 197, 370, 690, 3001, 3375
Tsp45I GTSAC 5 cut(s) 197, 370, 690, 3001, 3375
TspGWI ACGGA 8 cut(s) 76, 232, 480, 678, 1141, 3339, 4382, 4743
TspMI CCCGGG 1 cut(s) 3479
TspRI CASTG 5 cut(s) 159, 361, 2095, 2380, 2708
Tth111I GACNNNGTC 1 cut(s) 1939
Van91I CCANNNNNTGG 2 cut(s) 716, 3776
VneI GTGCAC 1 cut(s) 3162
VpaK11BI GGWCC 7 cut(s) 467, 1907, 2629, 3022, 3068, 3145, 3767
XagI CCTNNNNNAGG 2 cut(s) 2462, 4377
XapI RAATTY 4 cut(s) 771, 790, 3109, 4850
XbaI TCTAGA 2 cut(s) 335, 2638
XceI RCATGY 3 cut(s) 520, 830, 3764
XhoI CTCGAG 2 cut(s) 348, 2238
XmaI CCCGGG 1 cut(s) 3479
XmiI GTMKAC 2 cut(s) 1736, 4411
ZraI GACGTC 1 cut(s) 4637
Zsp2I ATGCAT 1 cut(s) 3762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.