RLG00000018980

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002907
Physical Location & Seq
Reverse (-)
43945812 .. 43959924
14113 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018980

Sequence Viewer

Length: 5211 bp
ATGGAGACTGGCTCGGAGGGAGAGAAGACGAGAGCTCAGGCTCAGGCTCAGGAAGGTGAGATCAGAACCAAGCGCAAAATGAAGACTCGTTCGCAGTTGGAAGTTCTTGAGCAAGCTTATGCAGTTGAGCAATACCCGTCGGAGTCGGTGAGAGCTGAGCTGTCGGCGAAATTGGATCTTTCCGATCGGCAACTACAGATGTGGTTTTGCCACCGGAGATTGAAGGACCGTAAGCCGACGCCGACCACCGGGAAGCGGCAGAGGAAGGACTCTCCGGCTGGAACGACTGGAGTGGTTTCTGAGGAAATGGCGGGGAGTGAGCCGCCGCCGCCGCCGCAGCAGTTGATAATAGAGGCTCGAGCTGTTGCTTTTGTGGAGGCGCAGCTGGGGGAGCCATTCAGGGAGGATGGGCCAATACTGGGCTTGGAGTTTGATCCGTTGCCACCGGGTGCATTTGGTTCCTCCGTAGGAGTGGCAACTGTTGGGCAGCAGAAGCAATTTGGATGGCCATATTCTGCAAATATATATGAGCAGTCTAATGCTAAACCAATTAAGGGTGCTTCAAGGACTGTCCATGAATACCAGTTTCTTCCAGCAAAGCCAACTGTTAGGACAGACACTTATGAAAGGGCTGCCTCATCTCACCACTATGGTTCACCCACTGAATCTCGCCATGCAAGGGTTCCATTATCTACTGTGTCCTCGTTCGTTCATGGTAATGAACAAGTACCCACTGTGTATGGATTTCAAAGCCAGATGGCTGGACTAAATCTTTTGTCACAGCAAGGCAGGGCTGGTCACCTCTTGCCATCTGCTTCAGAAGAATATGGCAATGTTCATGGAAAGAACTTCACGAATATTACCATGGATGCTCATTTTAGTGCTCACCCGATTAATCAACTGAACAATCCTTTTATACCATTTGCCAGAAGAGTGGCTCATGACGAGGATGATGTTTTAAGATTGGAAAGGAAGCACAAGAGTGAAGAAGCAAGAATAATGCGGGAAGTTGAAGCCCATGAGAAAAGGATACGTAAGGAGCTTGAAAAACAAGATATCCTGAGACGAAAGAGAGAAGAGCAGATAAGGAAAGAAATGGAGAGGATTGATAGGGAGAGGAGGAAGGAACAAGAAAGGCTATTGCGTGAAAAGCAGAGAGAGGAGGAGAGATACCTAAGGGAGCAAAGGCGTGAACTGGAACGAAAGGAGAAATTCTTACAGAAGGAGTCAATAAGAGCTGACAAAATGAAACAAAAAGAAGAATTACGCAGAGAGAAAGAGGCAGCAAAGCTTAAAGCTGCTAATGAGAGAGCTATTGCTCGTAAAAATGCTAAAGAATCAATGGAACTCATTGAGGATGAATGCCTGGAACTCATGGAGTTAGCTGCATCAAGCAAGGGACTTCCTTCGATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGATATGCGGAGTACATTCCCACCTAAATCTGTGCAGTTGAAAAAACCTTTTGGTATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGGCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAACACCCGCTATGGGGGTGGGAGCTAATCAGAACTGCACCGCAAATCCTGGAGGCGGTCACCCTCAAATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACTTAAATCCACTGACATGGCCAGAAATATTGCGGCAATTTTCTGTATCAGCAGGATTTGGACCGCAACTGAAGAAGAGGGGTGTTGAACCAGCTTATCTTCATGAGGACAATGAGGGTAATGATGTCAAGGACATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTCACTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGAAAATCAAGGCATCGCTTGACTCCTGGAACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGAACTGCTCCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACAAGTGATTCCGAGGCAGTACTTTCTGCAGCCAGAGAAAGAATTCAAATATTCAATAGTAGGATTTTTGATGTTGAAGGAGCTGATGAAGCTGAAAGAGACGGAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGTTTGATGATTTAAGCACTGAAATAAATCCAAAGATAGTAGCTCAGAATTCTGAAGAAGCAAGAAAAGTCAGTCACGGAATATCTTTGGAAAGTAGAAAAGGAAGTTACGTGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAGAAGGAATTCCTTCAATCAATTCTGAAGCGTTCGTCAAGGTTGAAGACACTGGTTGTTCCTTGGATGATTCTGTTGACGCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAACCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCATTAATTGGTGTGGCAATAGAAGGAAACTTGATTCGAATTGTTCTCGAGGAACGTTTAGAAGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGCGCAAGTTGATAAACGGCGTCTAAAAGAAGAACATGTCATCAAAATGCAATATACCCCTTTCATTGGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCTTCAGAAGATAGGCAAAGTCCATCTCCTATCGTTGATGAGAAAAACATTGAGATGACGGTGAAGCTTTCCGTCCTGCAAGAGCAGATAGTTGATCCACAAAATGAGCAAAAGTATATCAACAGTTTTCCTTCTGTAGGAAACCTCCAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTATTGCTGATAAGTCACACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCATTGCCTCTTGGTCAGGATCGTAGGTATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGCAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGCTCGTGGGTATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTCAAGGAAACTGTTAGAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATTAAAGACACTGCTAAAACAGAAGATGTTGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACGGACAGCCCTACCAGCTCTGTTTCTGTTGCTGACTCCGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAATGAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGACCTGGAGAGGTGGATTTTGAAAGAAAGTGTTGGTTCTTCAATATTATGTGCTGACAAAAATGGGAAAAGAAGGTGTCCCCAGCTACTGGACATCTGTAATTCTTGTTGTGGAATTTTTTATTTTGAAGAGAATCACTGTCTTTCTTGCCATAGAACTTTTCATAAGGGCGAAATAGATTTTTCACAACATGTGGTACTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGAGTTCTTCTCCCCTTAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACTGACATGAACCGTAAATCTTGGGGTAGAAAGTTACATTCCTCGTCAACAGCTGAACACGTTCTCCAGGTTCTGGCAACGTTGGAGAGTGCTGTAAAGATAGAATATTTATCTTCAAACTTTGAGACTACCGGTGAGCTGCTATATTCCTCAAATGAAAAAGGACGTGTTAGTAGCAATTCTTCTTCTCCTGATAGCGTTGCTGTGCTTCCATGGATACCACTTACAACAGCTGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCGGCCATCTCAAACATGCTACAGCAGAAGCTGGCGACTCAGAAGGACAAGGAATCTGGGGATTTTATAAAGCTTCCAACAAGATCTGCTGTTATGAAAAGTTCCAAGAATGAGGAGATCTCAGGAACTCCACATCAATCTGAAGATTCTAAAGAAGGCCACTGGGAGTATCTGGGGAGTGGACTTACCGGCTCTGGCCATGGACGAGGCCGTGGTCAAACTCGTGGTAGGCGAGTGCCAAAAAAGGCAATTGGTTTAAGGGGCAATCCTAGGAAGAAGAGTAGAGTTACAGCCAAAGATAAATTAGTACAGGTGCAAGTACTGAAATGGAAAGGTAGACCACGTGGACGAGGAGGGCGAAAGCGTGGAAATCGGAGCATTAGAGGCAAGGAGACAACGTTTAACAGGATAATTAAGATTGGTGGGGAGAGAACTGGCCCCAAAGACAGTGTCTATTATAAATCACCAGAGGACTTGGTTGAGGAGAAATGGAATGTAGGTGAAACTGCGGCAGAAAATGCAAGCGGTTCTGGAGGGTCAGAGTATGAAGTGGAAAATGGGGATGCATCAGGAGATGAATTTGATCATGTGCATGTGGATGACTATGCCGGTGGCTTCAATGGCAAGTCTGATGAGTTATTGGAGGGAGGCAAATATAACGTGGAGGGTGACGAAGATGATGTCGACGATGAGGTGGATGACGATGAAGGTGAAGGAGCACAAGATTCAAAAGGGGATTTTGATGGTGCAAGATATATCAATGGAGAGTGGGATGAAGAGGAACACATAACTGATGATGGACAGCAAGACATGGAACTTGGTGAAGGTACCACATCTTCGTCTTCAGACTATAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1737

Amino Acids

194.86

Weight (kDa)

5.67

Isoelectric Point (pI)

49.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 22 - 78 3.6e-16 Homeodomain
DDT PF02791 513 - 568 1.3e-18 DDT domain
HARE-HTH PF05066 694 - 761 1.2e-17 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 911 - 954 1e-09 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1094 - 1167 4.3e-22 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4418, 4809
Acc65I GGTACC 1 cut(s) 5177
AccB1I GGYRCC 1 cut(s) 5177
AccB7I CCANNNNNTGG 2 cut(s) 3835, 4159
AccI GTMKAC 2 cut(s) 4687, 5034
AclI AACGTT 3 cut(s) 2866, 4166, 4748
AcoI YGGCCR 5 cut(s) 506, 1604, 1859, 4353, 4546
AcsI RAATTY 8 cut(s) 1211, 1622, 2343, 2485, 2592, 3861, 3970, 4928
AcvI CACGTG 1 cut(s) 4694
AcyI GRCGYC 2 cut(s) 239, 2929
AdeI CACNNNGTG 1 cut(s) 449
AflIII ACRYGT 4 cut(s) 2944, 3937, 4144, 4252
AgeI ACCGGT 1 cut(s) 4217
AjiI CACGTC 1 cut(s) 4253
AjnI CCWGG 8 cut(s) 1365, 1768, 2077, 2563, 2731, 3358, 3750, 4152
AleI CACNNNNGTG 1 cut(s) 981
AloI GAACNNNNNNTCC 2 cut(s) 4134, 4166
Alw21I GWGCWC 5 cut(s) 37, 886, 2287, 3680, 5071
Alw26I GTCTC 6 cut(s) 1057, 1422, 2394, 2562, 4205, 4736
AlwNI CAGNNNCTG 5 cut(s) 2998, 3835, 4159, 4322, 4381
Ama87I CYCGRG 3 cut(s) 357, 2858, 3994
ApoI RAATTY 8 cut(s) 1211, 1622, 2343, 2485, 2592, 3861, 3970, 4928
ArsI GACNNNNNNTTYG 4 cut(s) 4785, 4817, 5016, 5048
AseI ATTAAT 2 cut(s) 894, 2816
AsiGI ACCGGT 1 cut(s) 4217
Asp700I GAANNNNTTC 6 cut(s) 1626, 2343, 2592, 2596, 4071, 4146
Asp718I GGTACC 1 cut(s) 5177
AspA2I CCTAGG 1 cut(s) 4619
AspLEI GCGC 4 cut(s) 75, 382, 2796, 2914
AspS9I GGNCC 6 cut(s) 226, 410, 1901, 2131, 3179, 4787
AsuC2I CCSGG 4 cut(s) 250, 447, 3207, 3551
AsuII TTCGAA 1 cut(s) 2848
AvaI CYCGRG 3 cut(s) 357, 2858, 3994
AvaII GGWCC 3 cut(s) 226, 1901, 3179
AvrII CCTAGG 1 cut(s) 4619
AxyI CCTNAGG 3 cut(s) 1175, 2573, 4010
BalI TGGCCA 4 cut(s) 508, 1606, 1861, 4548
BamHI GGATCC 1 cut(s) 2431
BanI GGYRCC 1 cut(s) 5177
BanII GRGCYC 2 cut(s) 37, 3680
BarI GAAGNNNNNNTAC 4 cut(s) 4252, 4284, 5170, 5202
BauI CACGAG 2 cut(s) 3456, 4572
BbrPI CACGTG 1 cut(s) 4694
BbsI GAAGAC 5 cut(s) 32, 89, 2637, 3503, 5184
Bbv12I GWGCWC 5 cut(s) 37, 886, 2287, 3680, 5071
BceAI ACGGC 2 cut(s) 2942, 4545
BcgI CGANNNNNNTGC 2 cut(s) 3377, 3411
BciT130I CCWGG 8 cut(s) 1367, 1770, 2079, 2565, 2733, 3360, 3752, 4154
BciVI GTATCC 2 cut(s) 1023, 4296
BclI TGATCA 2 cut(s) 2698, 4933
BcnI CCSGG 4 cut(s) 250, 447, 3207, 3551
BcoDI GTCTC 6 cut(s) 1057, 1422, 2394, 2562, 4205, 4736
BfaI CTAG 4 cut(s) 1653, 2232, 3701, 4620
BfmI CTRYAG 7 cut(s) 194, 2290, 2328, 2667, 3144, 4370, 5200
BfoI RGCGCY 1 cut(s) 2797
BfuI GTATCC 2 cut(s) 1023, 4296
BglII AGATCT 2 cut(s) 4433, 4467
BlnI CCTAGG 1 cut(s) 4619
BlpI GCTNAGC 1 cut(s) 156
BmcAI AGTACT 4 cut(s) 2322, 2771, 3170, 4671
Bme18I GGWCC 3 cut(s) 226, 1901, 3179
BmeT110I CYCGRG 3 cut(s) 357, 2858, 3994
BmgBI CACGTC 1 cut(s) 4253
BmgT120I GGNCC 6 cut(s) 226, 410, 1901, 2131, 3179, 4787
BmiI GGNNCC 6 cut(s) 393, 460, 684, 2433, 4789, 5179
BmrI ACTGGG 2 cut(s) 428, 4522
BmsI GCATC 9 cut(s) 859, 1397, 2074, 2410, 3442, 3452, 4339, 4903, 4925
BmuI ACTGGG 2 cut(s) 428, 4522
BpiI GAAGAC 5 cut(s) 32, 89, 2637, 3503, 5184
BplI GAGNNNNNCTC 7 cut(s) 28, 2245, 2277, 3988, 4020, 4454, 4486
BpmI CTGGAG 6 cut(s) 309, 1791, 2547, 3773, 4136, 4902
Bpu10I CCTNAGC 3 cut(s) 36, 42, 48
Bpu1102I GCTNAGC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 2848
BpuEI CTTGAG 2 cut(s) 128, 2138
BpuMI CCSGG 4 cut(s) 250, 447, 3207, 3551
Bsa29I ATCGAT 1 cut(s) 3283
BsaAI YACGTR 5 cut(s) 1034, 2282, 2548, 3989, 4694
BsaHI GRCGYC 2 cut(s) 239, 2929
BsaI GGTCTC 1 cut(s) 2562
BsaWI WCCGGW 2 cut(s) 213, 4217
BsaXI ACNNNNNCTCC 6 cut(s) 1989, 2019, 4134, 4164, 4733, 4763
Bse118I RCCGGY 3 cut(s) 4217, 4538, 4958
Bse21I CCTNAGG 3 cut(s) 1175, 2573, 4010
Bse3DI GCAATG 2 cut(s) 838, 3287
BseBI CCWGG 8 cut(s) 1367, 1770, 2079, 2565, 2733, 3360, 3752, 4154
BseCI ATCGAT 1 cut(s) 3283
BseMI GCAATG 2 cut(s) 838, 3287
BseRI GAGGAG 9 cut(s) 1132, 1175, 1178, 1550, 2804, 3281, 4478, 4716, 4847
BseYI CCCAGC 2 cut(s) 385, 3828
BsgI GTGCAG 2 cut(s) 1511, 1741
Bsh1285I CGRYCG 2 cut(s) 187, 3397
BshNI GGYRCC 1 cut(s) 5177
BshTI ACCGGT 1 cut(s) 4217
BshVI ATCGAT 1 cut(s) 3283
BsiEI CGRYCG 2 cut(s) 187, 3397
BsiHKAI GWGCWC 5 cut(s) 37, 886, 2287, 3680, 5071
BsiHKCI CYCGRG 3 cut(s) 357, 2858, 3994
BsiSI CCGG 9 cut(s) 214, 249, 275, 446, 3207, 3550, 4218, 4539, 4959
BslFI GGGAC 3 cut(s) 1413, 2249, 3810
BsmAI GTCTC 6 cut(s) 1057, 1422, 2394, 2562, 4205, 4736
BsmBI CGTCTC 2 cut(s) 1057, 2394
BsmFI GGGAC 3 cut(s) 1413, 2249, 3810
BsmI GAATGC 2 cut(s) 1367, 3541
Bso31I GGTCTC 1 cut(s) 2562
BsoBI CYCGRG 3 cut(s) 357, 2858, 3994
Bsp119I TTCGAA 1 cut(s) 2848
Bsp1286I GDGCHC 5 cut(s) 37, 886, 2287, 3680, 5071
Bsp1720I GCTNAGC 1 cut(s) 156
Bsp19I CCATGG 4 cut(s) 864, 2741, 4298, 4549
BspDI ATCGAT 1 cut(s) 3283
BspHI TCATGA 4 cut(s) 940, 1639, 1942, 2695
BspLI GGNNCC 6 cut(s) 393, 460, 684, 2433, 4789, 5179
BspMAI CTGCAG 2 cut(s) 2332, 2671
BspQI GCTCTTC 2 cut(s) 1071, 4077
BspT104I TTCGAA 1 cut(s) 2848
BspT107I GGYRCC 1 cut(s) 5177
BspTNI GGTCTC 1 cut(s) 2562
BsrDI GCAATG 2 cut(s) 838, 3287
BsrFI RCCGGY 3 cut(s) 4217, 4538, 4958
BssAI RCCGGY 3 cut(s) 4217, 4538, 4958
BssNI GRCGYC 2 cut(s) 239, 2929
BssSI CACGAG 2 cut(s) 3456, 4572
BssT1I CCWWGG 7 cut(s) 864, 1520, 2646, 2741, 4298, 4549, 4619
Bst2BI CACGAG 2 cut(s) 3456, 4572
Bst2UI CCWGG 8 cut(s) 1367, 1770, 2079, 2565, 2733, 3360, 3752, 4154
Bst6I CTCTTC 8 cut(s) 925, 1071, 1910, 3414, 3870, 4077, 4622, 5121
BstACI GRCGYC 2 cut(s) 239, 2929
BstAPI GCANNNNNTGC 2 cut(s) 2810, 4868
BstBAI YACGTR 5 cut(s) 1034, 2282, 2548, 3989, 4694
BstBI TTCGAA 1 cut(s) 2848
BstC8I GCNNGC 3 cut(s) 114, 4383, 4873
BstDSI CCRYGG 5 cut(s) 864, 2741, 4298, 4549, 4561
BstEII GGTNACC 2 cut(s) 797, 1778
BstENI CCTNNNNNAGG 1 cut(s) 3144
BstH2I RGCGCY 1 cut(s) 2797
BstHHI GCGC 4 cut(s) 75, 382, 2796, 2914
BstMAI GTCTC 6 cut(s) 1057, 1422, 2394, 2562, 4205, 4736
BstMCI CGRYCG 2 cut(s) 187, 3397
BstNI CCWGG 8 cut(s) 1367, 1770, 2079, 2565, 2733, 3360, 3752, 4154
BstNSI RCATGY 4 cut(s) 2948, 3941, 4369, 4946
BstPI GGTNACC 2 cut(s) 797, 1778
BstSFI CTRYAG 7 cut(s) 194, 2290, 2328, 2667, 3144, 4370, 5200
BstSNI TACGTA 1 cut(s) 1034
BstV2I GAAGAC 5 cut(s) 32, 89, 2637, 3503, 5184
BstX2I RGATCY 7 cut(s) 175, 1688, 2182, 2431, 3372, 4433, 4467
BstXI CCANNNNNNTGG 3 cut(s) 761, 934, 1857
BstYI RGATCY 7 cut(s) 175, 1688, 2182, 2431, 3372, 4433, 4467
Bsu15I ATCGAT 1 cut(s) 3283
Bsu36I CCTNAGG 3 cut(s) 1175, 2573, 4010
BsuI GTATCC 2 cut(s) 1023, 4296
BsuTUI ATCGAT 1 cut(s) 3283
BtgI CCRYGG 5 cut(s) 864, 2741, 4298, 4549, 4561
BtgZI GCGATG 1 cut(s) 2051
BtrI CACGTC 1 cut(s) 4253
BtsI GCAGTG 4 cut(s) 1999, 2913, 3570, 3622
Cac8I GCNNGC 3 cut(s) 114, 4383, 4873
CaiI CAGNNNCTG 5 cut(s) 2998, 3835, 4159, 4322, 4381
CciI TCATGA 4 cut(s) 940, 1639, 1942, 2695
CfoI GCGC 4 cut(s) 75, 382, 2796, 2914
Cfr10I RCCGGY 3 cut(s) 4217, 4538, 4958
Cfr13I GGNCC 6 cut(s) 226, 410, 1901, 2131, 3179, 4787
ClaI ATCGAT 1 cut(s) 3283
CseI GACGC 3 cut(s) 247, 2672, 2918
CspAI ACCGGT 1 cut(s) 4217
DraI TTTAAA 2 cut(s) 2889, 3735
DraIII CACNNNGTG 1 cut(s) 449
EaeI YGGCCR 5 cut(s) 506, 1604, 1859, 4353, 4546
Eam1104I CTCTTC 8 cut(s) 925, 1071, 1910, 3414, 3870, 4077, 4622, 5121
EarI CTCTTC 8 cut(s) 925, 1071, 1910, 3414, 3870, 4077, 4622, 5121
Ecl136II GAGCTC 2 cut(s) 35, 3678
Eco105I TACGTA 1 cut(s) 1034
Eco130I CCWWGG 7 cut(s) 864, 1520, 2646, 2741, 4298, 4549, 4619
Eco24I GRGCYC 2 cut(s) 37, 3680
Eco31I GGTCTC 1 cut(s) 2562
Eco32I GATATC 2 cut(s) 1057, 3743
Eco47I GGWCC 3 cut(s) 226, 1901, 3179
Eco53kI GAGCTC 2 cut(s) 35, 3678
Eco72I CACGTG 1 cut(s) 4694
Eco81I CCTNAGG 3 cut(s) 1175, 2573, 4010
Eco88I CYCGRG 3 cut(s) 357, 2858, 3994
Eco91I GGTNACC 2 cut(s) 797, 1778
EcoICRI GAGCTC 2 cut(s) 35, 3678
EcoNI CCTNNNNNAGG 1 cut(s) 3144
EcoO109I RGGNCCY 1 cut(s) 2131
EcoO65I GGTNACC 2 cut(s) 797, 1778
EcoRI GAATTC 5 cut(s) 1622, 2343, 2485, 2592, 3970
EcoRII CCWGG 8 cut(s) 1365, 1768, 2077, 2563, 2731, 3358, 3750, 4152
EcoRV GATATC 2 cut(s) 1057, 3743
EcoT14I CCWWGG 7 cut(s) 864, 1520, 2646, 2741, 4298, 4549, 4619
EcoT22I ATGCAT 1 cut(s) 4918
EcoT38I GRGCYC 2 cut(s) 37, 3680
ErhI CCWWGG 7 cut(s) 864, 1520, 2646, 2741, 4298, 4549, 4619
Esp3I CGTCTC 2 cut(s) 1057, 2394
FalI AAGNNNNNCTT 2 cut(s) 1826, 1858
FaqI GGGAC 3 cut(s) 1413, 2249, 3810
FauI CCCGC 3 cut(s) 304, 996, 1735
FauNDI CATATG 1 cut(s) 1804
FbaI TGATCA 2 cut(s) 2698, 4933
FblI GTMKAC 2 cut(s) 4687, 5034
FriOI GRGCYC 2 cut(s) 37, 3680
FspBI CTAG 4 cut(s) 1653, 2232, 3701, 4620
GlaI GCGC 4 cut(s) 74, 381, 2795, 2913
GsaI CCCAGC 2 cut(s) 389, 3832
GsuI CTGGAG 6 cut(s) 309, 1791, 2547, 3773, 4136, 4902
HaeII RGCGCY 1 cut(s) 2797
HapII CCGG 9 cut(s) 214, 249, 275, 446, 3207, 3550, 4218, 4539, 4959
HgaI GACGC 3 cut(s) 247, 2672, 2918
HhaI GCGC 4 cut(s) 75, 382, 2796, 2914
Hin1I GRCGYC 2 cut(s) 239, 2929
Hin6I GCGC 4 cut(s) 73, 380, 2794, 2912
HinP1I GCGC 4 cut(s) 73, 380, 2794, 2912
HincII GTYRAC 3 cut(s) 2662, 4134, 5035
HindII GTYRAC 3 cut(s) 2662, 4134, 5035
HindIII AAGCTT 5 cut(s) 114, 1289, 1632, 3074, 4421
HpaII CCGG 9 cut(s) 214, 249, 275, 446, 3207, 3550, 4218, 4539, 4959
Hpy166II GTNNAC 9 cut(s) 656, 1193, 2279, 2662, 4134, 4532, 4688, 4697, 5035
Hpy8I GTNNAC 9 cut(s) 656, 1193, 2279, 2662, 4134, 4532, 4688, 4697, 5035
Hpy99I CGWCG 3 cut(s) 142, 241, 5039
Hsp92I GRCGYC 2 cut(s) 239, 2929
HspAI GCGC 4 cut(s) 73, 380, 2794, 2912
KpnI GGTACC 1 cut(s) 5181
Ksp22I TGATCA 2 cut(s) 2698, 4933
LguI GCTCTTC 2 cut(s) 1071, 4077
LweI GCATC 9 cut(s) 859, 1397, 2074, 2410, 3442, 3452, 4339, 4903, 4925
MaeI CTAG 4 cut(s) 1653, 2232, 3701, 4620
MfeI CAATTG 2 cut(s) 3693, 4599
MflI RGATCY 7 cut(s) 175, 1688, 2182, 2431, 3372, 4433, 4467
MhlI GDGCHC 5 cut(s) 37, 886, 2287, 3680, 5071
MlsI TGGCCA 4 cut(s) 508, 1606, 1861, 4548
MluNI TGGCCA 4 cut(s) 508, 1606, 1861, 4548
MlyI GAGTC 8 cut(s) 79, 152, 263, 1235, 2068, 2231, 3653, 4381
MmeI TCCRAC 6 cut(s) 78, 120, 3380, 3651, 4149, 4451
Mox20I TGGCCA 4 cut(s) 508, 1606, 1861, 4548
Mph1103I ATGCAT 1 cut(s) 4918
MroXI GAANNNNTTC 6 cut(s) 1626, 2343, 2592, 2596, 4071, 4146
MscI TGGCCA 4 cut(s) 508, 1606, 1861, 4548
Msp20I TGGCCA 4 cut(s) 508, 1606, 1861, 4548
MspA1I CMGCKG 3 cut(s) 385, 4139, 4319
MspI CCGG 9 cut(s) 214, 249, 275, 446, 3207, 3550, 4218, 4539, 4959
MunI CAATTG 2 cut(s) 3693, 4599
Mva1269I GAATGC 2 cut(s) 1367, 3541
MvaI CCWGG 8 cut(s) 1367, 1770, 2079, 2565, 2733, 3360, 3752, 4154
NciI CCSGG 4 cut(s) 250, 447, 3207, 3551
NcoI CCATGG 4 cut(s) 864, 2741, 4298, 4549
NdeI CATATG 1 cut(s) 1804
NlaIV GGNNCC 6 cut(s) 393, 460, 684, 2433, 4789, 5179
NmuCI GTSAC 9 cut(s) 777, 797, 1778, 2510, 2581, 3225, 3254, 5018, 5204
NsiI ATGCAT 1 cut(s) 4918
NspI RCATGY 4 cut(s) 2948, 3941, 4369, 4946
NspV TTCGAA 1 cut(s) 2848
OliI CACNNNNGTG 1 cut(s) 981
PaeR7I CTCGAG 3 cut(s) 357, 2858, 3994
PagI TCATGA 4 cut(s) 940, 1639, 1942, 2695
PciI ACATGT 2 cut(s) 2944, 3937
PciSI GCTCTTC 2 cut(s) 1071, 4077
PcsI WCGNNNNNNNCGW 3 cut(s) 1623, 4579, 4705
PctI GAATGC 2 cut(s) 1367, 3541
PdmI GAANNNNTTC 6 cut(s) 1626, 2343, 2592, 2596, 4071, 4146
PflFI GACNNNGTC 1 cut(s) 4799
PflMI CCANNNNNTGG 2 cut(s) 3835, 4159
PfoI TCCNGGA 3 cut(s) 1768, 2077, 2563
PinAI ACCGGT 1 cut(s) 4217
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 8 cut(s) 79, 151, 263, 1234, 2068, 2231, 3653, 4381
PmaCI CACGTG 1 cut(s) 4694
PmlI CACGTG 1 cut(s) 4694
PpsI GAGTC 8 cut(s) 79, 151, 263, 1234, 2068, 2231, 3653, 4381
Ppu21I YACGTR 5 cut(s) 1034, 2282, 2548, 3989, 4694
PscI ACATGT 2 cut(s) 2944, 3937
PshBI ATTAAT 2 cut(s) 894, 2816
PsiI TTATAA 2 cut(s) 4418, 4809
Psp124BI GAGCTC 2 cut(s) 37, 3680
Psp1406I AACGTT 3 cut(s) 2866, 4166, 4748
Psp6I CCWGG 8 cut(s) 1365, 1768, 2077, 2563, 2731, 3358, 3750, 4152
PspCI CACGTG 1 cut(s) 4694
PspEI GGTNACC 2 cut(s) 797, 1778
PspFI CCCAGC 2 cut(s) 385, 3828
PspGI CCWGG 8 cut(s) 1365, 1768, 2077, 2563, 2731, 3358, 3750, 4152
PspN4I GGNNCC 6 cut(s) 393, 460, 684, 2433, 4789, 5179
PspPI GGNCC 6 cut(s) 226, 410, 1901, 2131, 3179, 4787
PspXI VCTCGAGB 2 cut(s) 357, 3994
PstI CTGCAG 2 cut(s) 2332, 2671
PstNI CAGNNNCTG 5 cut(s) 2998, 3835, 4159, 4322, 4381
PsuI RGATCY 7 cut(s) 175, 1688, 2182, 2431, 3372, 4433, 4467
PsyI GACNNNGTC 1 cut(s) 4799
PvuI CGATCG 1 cut(s) 187
PvuII CAGCTG 3 cut(s) 385, 4139, 4319
SacI GAGCTC 2 cut(s) 37, 3680
SalI GTCGAC 1 cut(s) 5033
SapI GCTCTTC 2 cut(s) 1071, 4077
Sau96I GGNCC 6 cut(s) 226, 410, 1901, 2131, 3179, 4787
ScaI AGTACT 4 cut(s) 2322, 2771, 3170, 4671
SchI GAGTC 8 cut(s) 79, 152, 263, 1235, 2068, 2231, 3653, 4381
SduI GDGCHC 5 cut(s) 37, 886, 2287, 3680, 5071
SfaNI GCATC 9 cut(s) 859, 1397, 2074, 2410, 3442, 3452, 4339, 4903, 4925
SfcI CTRYAG 7 cut(s) 194, 2290, 2328, 2667, 3144, 4370, 5200
Sfr274I CTCGAG 3 cut(s) 357, 2858, 3994
SfuI TTCGAA 1 cut(s) 2848
SinI GGWCC 3 cut(s) 226, 1901, 3179
SlaI CTCGAG 3 cut(s) 357, 2858, 3994
SmlI CTYRAG 5 cut(s) 107, 357, 2117, 2858, 3994
SmoI CTYRAG 5 cut(s) 107, 357, 2117, 2858, 3994
SnaBI TACGTA 1 cut(s) 1034
SspI AATATT 6 cut(s) 859, 1869, 2352, 3507, 3792, 4193
SspMI CTAG 4 cut(s) 1653, 2232, 3701, 4620
SstI GAGCTC 2 cut(s) 37, 3680
StyI CCWWGG 7 cut(s) 864, 1520, 2646, 2741, 4298, 4549, 4619
TatI WGTACW 6 cut(s) 1469, 2320, 2769, 3168, 4657, 4669
TauI GCSGC 9 cut(s) 259, 325, 328, 331, 334, 337, 1876, 4355, 4862
TseFI GTSAC 9 cut(s) 777, 797, 1778, 2510, 2581, 3225, 3254, 5018, 5204
Tsp45I GTSAC 9 cut(s) 777, 797, 1778, 2510, 2581, 3225, 3254, 5018, 5204
TspGWI ACGGA 6 cut(s) 426, 454, 2418, 2529, 3070, 3641
Tth111I GACNNNGTC 1 cut(s) 4799
Van91I CCANNNNNTGG 2 cut(s) 3835, 4159
VpaK11BI GGWCC 3 cut(s) 226, 1901, 3179
VspI ATTAAT 2 cut(s) 894, 2816
XagI CCTNNNNNAGG 1 cut(s) 3144
XapI RAATTY 8 cut(s) 1211, 1622, 2343, 2485, 2592, 3861, 3970, 4928
XceI RCATGY 4 cut(s) 2948, 3941, 4369, 4946
XhoI CTCGAG 3 cut(s) 357, 2858, 3994
XmaJI CCTAGG 1 cut(s) 4619
XmiI GTMKAC 2 cut(s) 4687, 5034
XmnI GAANNNNTTC 6 cut(s) 1626, 2343, 2592, 2596, 4071, 4146
XspI CTAG 4 cut(s) 1653, 2232, 3701, 4620
ZrmI AGTACT 4 cut(s) 2322, 2771, 3170, 4671
Zsp2I ATGCAT 1 cut(s) 4918
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.