Rh2AG328000

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002907
Physical Location & Seq
Reverse (-)
47086482 .. 47100580
14099 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG328000.1

Sequence Viewer

Length: 5166 bp
ATGGAGACTGGCTCGGAGGGAGAGAAGACGAGAGCTGAGGCTCAGGCTCAGGAAGGTGAGATCAGAACCAAGCGCAAAATGAAGACTCGTTCGCAGTTGGAAGTTCTTGAGCAAGCTTATGCAGCTGAGCAATACCCGTCGGAGTCGGTGAGAGCTGAGCTGTCGGCGAAATTGGATCTTTCCGATCGGCAACTACAGATGTGGTTCTGCCACCGGAGATTGAAGGACCGTAAGCCGACGCCGCCGACGACCGGGAAGCGGCAGAGGAAGGACTCTCCGGCTGGAACGACTGGAGTGGTTTCTGAGGAAATGGCGGGGAGTGAGCCGCCGCCGCAGCAGTTGATAATAGAGGCTCGAGCTGTTGCTTTTGTGGAGGCGCAGCTGGGAGAGCCATTCAGGGAGGACGGGCCGATACTGGGCTTGGAGTTTGATCCGTTGCCACCGGGCGCATTTGGTTCCTCCGTAGGAGTGGCAACTGTTGGGCAGCAGAAGCAATCGGGATGGCCATATTCTGCAAATATATATGAGCAGTCTAATGCTAAACCAATTAAGGGTGCTTCAAGGACTGTCCATGAATACCAGTTTCTTCCAGAAAAGCCAACTGTTAGGACAGACACTTATGAAAGGGCTGCCCCATCTCACCACTATGGTTCACCCACTGAATCTCGCCATGCAAGGATTCCATTATCTACTGTGTCCTCGTTCATTCATGGTAATGAACAAGTGCCCACTGTATATCGATTTCAAAGCCAGATGGCTGGGCTAAATCTTTTGTCACAGCAAGGCAGGGCTGGTCACCTCTTGCCATCTGCTTCAGAAGAATATGGCAATGTTCATGGAAAGAACTTCACGAATATTACCATGGATGCTCATTTTAGTGCTCACCCAATTAATCAACTGAACAATCCTTTTATACCATTTGCCAGAAGTGTGGCTCATGACGAGGATGATGTTTTAAGATTGGAAAGGAAGCACAAGAGTGAAGAAACAAGAATAATGCGGGAAGTTGAAGCCCATGAGAAAAGGATACGTAAAGAGCTTGAAAAACAAGATATCCTGAGACGAAAGAGAGAAGAGCAGATAAGGAAAGAAATGGAGAGGATTGATCGGGAGAGGAGGAAGGAAGAAGAAAGGCTATTGCGTGAAAAGCAGAGAGAGGAGGAGAGATACCTAAGGGAGCAAAGGCGTGAACTGGAGCGAAAGGAGAAATTCTTACAGAAGGAGTCAATAAGAGCTGAGAAAATGAGACAAAAAGAAGAATTACGCAGAGAGAAAGAGGCAGCAAGGCTTAAAGCTGCTAATGAGAGAGCTATTGCTCGTAAAAATGCTAAAGAATCAATGGAACTCATTGAGGATGAATGCCTGGAACTCATGGAGTTAGCTGCATCAAGCAAGGAGGTTCCTTCCATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGAGATGCGGAGTACATTCCCACCCAAATCTGTGCAGTTGAAAAAACCTTTTGGTATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGCCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAATACCCTCTATGGGGGTGGGAGCTAATCAGAACTGCGCTGCAAATCCTGGAGGCGGTCACCCTCAAATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACTTAAATCCACTGACATGGCCAGAAATATTGCGGCAATTTTCTGTATCAGCAGGATTTGGACCGCAACTGAAGAAGAGGAATGTTGAACCAGCTTATCTTCATGAGGACAATGAGGGTAATGATGTCAAGGATATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTCGCTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGCAAATCAAGGCATTGCTTGACTCCTGGCACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGAACTGCACCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACAAGTGATTCCGAGGCAGTACTTTCGGCAGCCAGAGAAAGAATTCAAATATTCAATAGTAGGATTTTTGATGTTGAAGGAGCTGATGAAGCTGAAAGAGACGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGTTTGATGATTTAGGCACTGAAATAAATCCAAAGATAGTAGCTCAGAATTCTGAAGAAGCAAGAAAAGTCAGTGACGGAATATCTTTGGAAAGTGGAAAAGGAAGTTATGAGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAAAAGGAATTCCTTCAATCAATTCTGAAGCGTCTATCAAGGTTGAAGACGCTGGTTGTTCCTTGGATTCTGTTGACCCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAACCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCATTAATTGGTGTGGCAATAGAAGGAAATTCGATTCGAATTGTTCTCGAGGAACGTTTAGATGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGCGCAAGTTGATAAACGGCGTCTAAAAGAAGAACATGTCATTAAAATGCAATATACCCCTTTCATTGGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCTTCAGAAAATAGGCAAAGTCCATCTCCTACCATTGATGAGAAAAACATTGAGACGACGGTGAAGCTTTCCGTCCTGCAAGAGCAGATGGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCTTCTGTAGGAAACCTCCAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTATTGCTGATAAGTCGCACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCATTGCCTCTTGGTCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGCAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGTGCGTGGGTATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTCAAGGAAACTGTTAGAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATCAAAGACACTGCTAAAACAGAAGATGTTGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACAGATAGCCCTACCAGCTCTGTTTCTGTTGCTGACTCCGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAACGAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGATCTGGAGAGGTGGATTTTGAAAGAAAGTGTTGGTTCTTCAATATTATGTGCTGACAAAAATGGGGAAAAAAGGTGTCCCCAGCTACTGGACATCTGCAATTCTTGTCGTGGAATTTTTTATTTTGAAGAGAATCACTGTCATTCTTGCCATAGAACTTTTGGTAAGGGCGAAATAGATTTTTCACAACATGTGGTACTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGAGTTCTTCTCCCCTTAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAAATCTTGGGGTAGAAAGTTACATTCCTCGTCAACAGCTGAAGCCCTTCTCCAGGTTCTGGCATCGTTGGAGAGTGCTGTAAAGATAGAATATTTATCTTCAAACTTTGAGACTACCGGTGAGCTGCTATATTCCTCAAATGAAAAAGGACGTGTTACTAGCAATTCTTCTTCTCCTGATAGCGTTGCTGTGCTTCCATGGGTACCACTTACAACAGCTGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCGGCCATCTCAAACATGCTACAGCAGAAGCTGGTGACTCAGAAGGACAAGGAATCTGGGGATTTTAAAAAGCTTCCAACAAGATCTGCTGTCATGAAAAGTTCCAAGAATGAGGAGATCTCAGGAACTCCACATCAATCTGAAGATTCTAAAGAAGGCCACTGGGAGTATCTGGGGAGTGGACTTACCGGCTCTGGCCATGGACGAGGCCGTGGTCGAACTCGTGGTAGGCGAGTGCCAAAAAAGGCAATTGGTTTAAGGGGCAATCCTAGGAAGAAGAGTAGAGTTACAGCCAAAGATAAATTAGTACAGGTGCAAGTACTGAAATGGAAAGGCAGACCACGTGGACGAGGAGGGCGAAAGCGTGGAAATCGGAGCATTAGAGGCAAGGAGACAACATTTAACAGGATAATTAAGATTGGTGGGGAGAGAACTGGCCCCAAAGACAGTGTCTATTATAAATCACCAGATGACTTGGTTGAGGAGAATTGGAACGTAGGTGAAACTGTGGCAGAAAATGCAAGCGGTTCTGGAAGGTCAGAGTATGAAGTGGAAAATGGGGATGCATCAGGAGATGAATTTGATCATGTGCATGTGGATGACTTATTGGAGGGAGGCGAATATAACATGGAGGGTGACGAAGATGATGTCGACGATGAGGTGGATGACGATGAAGGTGAAGGAGCACAGGATTCAGATTTTGATGGTGCAAGATATATCAATGGAGAGTGGGATGAAGAGGAACACATAGCTGATGATGGACAGCAAGACATGGAACTTGGTGAAGGTACCAGATCTTCGTCTTCAGACTATAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1721

Amino Acids

193.49

Weight (kDa)

5.54

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 22 - 78 2.4e-16 Homeodomain
DDT PF02791 512 - 567 1.3e-18 DDT domain
HARE-HTH PF05066 693 - 760 1.2e-17 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 909 - 952 3.9e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1092 - 1165 1.7e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4803
Acc65I GGTACC 2 cut(s) 4297, 5132
AccB1I GGYRCC 2 cut(s) 4297, 5132
AccB7I CCANNNNNTGG 2 cut(s) 3829, 4153
AccI GTMKAC 1 cut(s) 4995
AclI AACGTT 1 cut(s) 2860
AcoI YGGCCR 5 cut(s) 503, 1601, 1856, 4347, 4540
AcsI RAATTY 9 cut(s) 1208, 1619, 2340, 2482, 2589, 2833, 3855, 3964, 4922
AcvI CACGTG 1 cut(s) 4688
AcyI GRCGYC 2 cut(s) 239, 2923
AflIII ACRYGT 3 cut(s) 2938, 3931, 4246
AgeI ACCGGT 1 cut(s) 4211
AjiI CACGTC 1 cut(s) 4247
AjnI CCWGG 7 cut(s) 1362, 1765, 2074, 2560, 2725, 3352, 4146
AleI CACNNNNGTG 1 cut(s) 978
Alw21I GWGCWC 4 cut(s) 883, 2284, 3674, 5032
Alw26I GTCTC 8 cut(s) 1054, 1240, 1419, 2391, 2559, 3050, 4199, 4730
AlwNI CAGNNNCTG 5 cut(s) 2992, 3829, 4153, 4316, 4375
Ama87I CYCGRG 3 cut(s) 354, 2852, 3988
ApoI RAATTY 9 cut(s) 1208, 1619, 2340, 2482, 2589, 2833, 3855, 3964, 4922
ArsI GACNNNNNNTTYG 4 cut(s) 4779, 4811, 4977, 5009
AseI ATTAAT 2 cut(s) 891, 2810
AsiGI ACCGGT 1 cut(s) 4211
Asp700I GAANNNNTTC 5 cut(s) 1623, 2340, 2593, 4065, 4140
Asp718I GGTACC 2 cut(s) 4297, 5132
AspA2I CCTAGG 1 cut(s) 4613
AspLEI GCGC 6 cut(s) 75, 379, 449, 1757, 2790, 2908
AspS9I GGNCC 7 cut(s) 226, 407, 1898, 2128, 3173, 4080, 4781
AsuC2I CCSGG 4 cut(s) 253, 444, 3201, 3545
AsuII TTCGAA 1 cut(s) 2842
AvaI CYCGRG 3 cut(s) 354, 2852, 3988
AvaII GGWCC 4 cut(s) 226, 1898, 3173, 4080
AvrII CCTAGG 1 cut(s) 4613
AxyI CCTNAGG 3 cut(s) 1172, 2570, 4004
BaeGI GKGCMC 1 cut(s) 729
BalI TGGCCA 4 cut(s) 505, 1603, 1858, 4542
BamHI GGATCC 1 cut(s) 2428
BanI GGYRCC 2 cut(s) 4297, 5132
BanII GRGCYC 1 cut(s) 3674
BarI GAAGNNNNNNTAC 2 cut(s) 5125, 5157
BauI CACGAG 1 cut(s) 4566
BbrPI CACGTG 1 cut(s) 4688
BbsI GAAGAC 5 cut(s) 32, 89, 2634, 3497, 5139
Bbv12I GWGCWC 4 cut(s) 883, 2284, 3674, 5032
BbvCI CCTCAGC 1 cut(s) 36
BceAI ACGGC 2 cut(s) 2936, 4539
BcgI CGANNNNNNTGC 4 cut(s) 2304, 2338, 3371, 3405
BciT130I CCWGG 7 cut(s) 1364, 1767, 2076, 2562, 2727, 3354, 4148
BciVI GTATCC 1 cut(s) 1020
BclI TGATCA 2 cut(s) 2692, 4927
BcnI CCSGG 4 cut(s) 253, 444, 3201, 3545
BcoDI GTCTC 8 cut(s) 1054, 1240, 1419, 2391, 2559, 3050, 4199, 4730
BfaI CTAG 5 cut(s) 1650, 2229, 3695, 4254, 4614
BfmI CTRYAG 6 cut(s) 194, 2287, 2661, 3138, 4364, 5155
BfoI RGCGCY 1 cut(s) 2791
BfuI GTATCC 1 cut(s) 1020
BglII AGATCT 4 cut(s) 3742, 4427, 4461, 5138
BlnI CCTAGG 1 cut(s) 4613
BlpI GCTNAGC 2 cut(s) 126, 156
BmcAI AGTACT 4 cut(s) 2319, 2765, 3164, 4665
Bme18I GGWCC 4 cut(s) 226, 1898, 3173, 4080
BmeT110I CYCGRG 3 cut(s) 354, 2852, 3988
BmgBI CACGTC 1 cut(s) 4247
BmgT120I GGNCC 7 cut(s) 226, 407, 1898, 2128, 3173, 4080, 4781
BmiI GGNNCC 6 cut(s) 457, 1401, 2430, 4299, 4783, 5134
BmrI ACTGGG 2 cut(s) 425, 4516
BmuI ACTGGG 2 cut(s) 425, 4516
BpiI GAAGAC 5 cut(s) 32, 89, 2634, 3497, 5139
BplI GAGNNNNNCTC 5 cut(s) 28, 3982, 4014, 4448, 4480
BpmI CTGGAG 6 cut(s) 312, 1214, 1788, 2544, 3767, 4130
Bpu10I CCTNAGC 3 cut(s) 36, 42, 48
Bpu1102I GCTNAGC 2 cut(s) 126, 156
Bpu14I TTCGAA 1 cut(s) 2842
BpuEI CTTGAG 2 cut(s) 128, 2135
BpuMI CCSGG 4 cut(s) 253, 444, 3201, 3545
Bsa29I ATCGAT 2 cut(s) 739, 3277
BsaAI YACGTR 4 cut(s) 1031, 2279, 3983, 4688
BsaHI GRCGYC 2 cut(s) 239, 2923
BsaI GGTCTC 1 cut(s) 2559
BsaWI WCCGGW 2 cut(s) 213, 4211
BsaXI ACNNNNNCTCC 2 cut(s) 1986, 2016
Bse118I RCCGGY 2 cut(s) 4211, 4532
Bse21I CCTNAGG 3 cut(s) 1172, 2570, 4004
Bse3DI GCAATG 3 cut(s) 835, 2062, 3281
BseBI CCWGG 7 cut(s) 1364, 1767, 2076, 2562, 2727, 3354, 4148
BseCI ATCGAT 2 cut(s) 739, 3277
BseMI GCAATG 3 cut(s) 835, 2062, 3281
BseRI GAGGAG 9 cut(s) 1129, 1172, 1175, 1547, 2798, 3275, 4472, 4710, 4841
BseSI GKGCMC 1 cut(s) 729
BseYI CCCAGC 3 cut(s) 382, 758, 3822
BsgI GTGCAG 2 cut(s) 1508, 2241
Bsh1285I CGRYCG 3 cut(s) 187, 252, 3391
BshNI GGYRCC 2 cut(s) 4297, 5132
BshTI ACCGGT 1 cut(s) 4211
BshVI ATCGAT 2 cut(s) 739, 3277
BsiEI CGRYCG 3 cut(s) 187, 252, 3391
BsiHKAI GWGCWC 4 cut(s) 883, 2284, 3674, 5032
BsiHKCI CYCGRG 3 cut(s) 354, 2852, 3988
BsiSI CCGG 8 cut(s) 214, 252, 278, 443, 3201, 3544, 4212, 4533
BslFI GGGAC 2 cut(s) 2246, 3804
BsmAI GTCTC 8 cut(s) 1054, 1240, 1419, 2391, 2559, 3050, 4199, 4730
BsmBI CGTCTC 3 cut(s) 1054, 2391, 3050
BsmFI GGGAC 2 cut(s) 2246, 3804
BsmI GAATGC 2 cut(s) 1364, 3535
Bso31I GGTCTC 1 cut(s) 2559
BsoBI CYCGRG 3 cut(s) 354, 2852, 3988
Bsp119I TTCGAA 1 cut(s) 2842
Bsp1286I GDGCHC 5 cut(s) 729, 883, 2284, 3674, 5032
Bsp1720I GCTNAGC 2 cut(s) 126, 156
Bsp19I CCATGG 4 cut(s) 861, 2735, 4292, 4543
BspDI ATCGAT 2 cut(s) 739, 3277
BspHI TCATGA 5 cut(s) 937, 1636, 1939, 2689, 4437
BspLI GGNNCC 6 cut(s) 457, 1401, 2430, 4299, 4783, 5134
BspMAI CTGCAG 1 cut(s) 2665
BspQI GCTCTTC 2 cut(s) 1068, 4071
BspT104I TTCGAA 1 cut(s) 2842
BspT107I GGYRCC 2 cut(s) 4297, 5132
BspTNI GGTCTC 1 cut(s) 2559
BsrDI GCAATG 3 cut(s) 835, 2062, 3281
BsrFI RCCGGY 2 cut(s) 4211, 4532
BssAI RCCGGY 2 cut(s) 4211, 4532
BssNI GRCGYC 2 cut(s) 239, 2923
BssSI CACGAG 1 cut(s) 4566
BssT1I CCWWGG 7 cut(s) 861, 1517, 2643, 2735, 4292, 4543, 4613
Bst2BI CACGAG 1 cut(s) 4566
Bst2UI CCWGG 7 cut(s) 1364, 1767, 2076, 2562, 2727, 3354, 4148
Bst6I CTCTTC 7 cut(s) 1068, 1907, 3408, 3864, 4071, 4616, 5076
BstACI GRCGYC 2 cut(s) 239, 2923
BstAPI GCANNNNNTGC 2 cut(s) 2804, 4862
BstBAI YACGTR 4 cut(s) 1031, 2279, 3983, 4688
BstBI TTCGAA 1 cut(s) 2842
BstC8I GCNNGC 2 cut(s) 114, 4867
BstDSI CCRYGG 5 cut(s) 861, 2735, 4292, 4543, 4555
BstEII GGTNACC 2 cut(s) 794, 1775
BstENI CCTNNNNNAGG 2 cut(s) 3138, 4145
BstH2I RGCGCY 1 cut(s) 2791
BstHHI GCGC 6 cut(s) 75, 379, 449, 1757, 2790, 2908
BstMAI GTCTC 8 cut(s) 1054, 1240, 1419, 2391, 2559, 3050, 4199, 4730
BstMCI CGRYCG 3 cut(s) 187, 252, 3391
BstNI CCWGG 7 cut(s) 1364, 1767, 2076, 2562, 2727, 3354, 4148
BstNSI RCATGY 4 cut(s) 2942, 3935, 4363, 4940
BstPI GGTNACC 2 cut(s) 794, 1775
BstSFI CTRYAG 6 cut(s) 194, 2287, 2661, 3138, 4364, 5155
BstSLI GKGCMC 1 cut(s) 729
BstSNI TACGTA 1 cut(s) 1031
BstV2I GAAGAC 5 cut(s) 32, 89, 2634, 3497, 5139
BstX2I RGATCY 9 cut(s) 175, 1685, 2179, 2428, 3366, 3742, 4427, 4461, 5138
BstXI CCANNNNNNTGG 3 cut(s) 758, 931, 1854
BstYI RGATCY 9 cut(s) 175, 1685, 2179, 2428, 3366, 3742, 4427, 4461, 5138
Bsu15I ATCGAT 2 cut(s) 739, 3277
Bsu36I CCTNAGG 3 cut(s) 1172, 2570, 4004
BsuI GTATCC 1 cut(s) 1020
BsuTUI ATCGAT 2 cut(s) 739, 3277
BtgI CCRYGG 5 cut(s) 861, 2735, 4292, 4543, 4555
BtrI CACGTC 1 cut(s) 4247
BtsI GCAGTG 4 cut(s) 1996, 2907, 3564, 3616
Cac8I GCNNGC 2 cut(s) 114, 4867
CaiI CAGNNNCTG 5 cut(s) 2992, 3829, 4153, 4316, 4375
CciI TCATGA 5 cut(s) 937, 1636, 1939, 2689, 4437
CfoI GCGC 6 cut(s) 75, 379, 449, 1757, 2790, 2908
Cfr10I RCCGGY 2 cut(s) 4211, 4532
Cfr13I GGNCC 7 cut(s) 226, 407, 1898, 2128, 3173, 4080, 4781
ClaI ATCGAT 2 cut(s) 739, 3277
CseI GACGC 4 cut(s) 247, 2601, 2639, 2912
CspAI ACCGGT 1 cut(s) 4211
DraI TTTAAA 3 cut(s) 2883, 3729, 4411
EaeI YGGCCR 5 cut(s) 503, 1601, 1856, 4347, 4540
Eam1104I CTCTTC 7 cut(s) 1068, 1907, 3408, 3864, 4071, 4616, 5076
EarI CTCTTC 7 cut(s) 1068, 1907, 3408, 3864, 4071, 4616, 5076
Ecl136II GAGCTC 1 cut(s) 3672
Eco105I TACGTA 1 cut(s) 1031
Eco130I CCWWGG 7 cut(s) 861, 1517, 2643, 2735, 4292, 4543, 4613
Eco24I GRGCYC 1 cut(s) 3674
Eco31I GGTCTC 1 cut(s) 2559
Eco32I GATATC 2 cut(s) 1054, 3737
Eco47I GGWCC 4 cut(s) 226, 1898, 3173, 4080
Eco53kI GAGCTC 1 cut(s) 3672
Eco72I CACGTG 1 cut(s) 4688
Eco81I CCTNAGG 3 cut(s) 1172, 2570, 4004
Eco88I CYCGRG 3 cut(s) 354, 2852, 3988
Eco91I GGTNACC 2 cut(s) 794, 1775
EcoICRI GAGCTC 1 cut(s) 3672
EcoNI CCTNNNNNAGG 2 cut(s) 3138, 4145
EcoO109I RGGNCCY 1 cut(s) 2128
EcoO65I GGTNACC 2 cut(s) 794, 1775
EcoRI GAATTC 5 cut(s) 1619, 2340, 2482, 2589, 3964
EcoRII CCWGG 7 cut(s) 1362, 1765, 2074, 2560, 2725, 3352, 4146
EcoRV GATATC 2 cut(s) 1054, 3737
EcoT14I CCWWGG 7 cut(s) 861, 1517, 2643, 2735, 4292, 4543, 4613
EcoT22I ATGCAT 1 cut(s) 4912
EcoT38I GRGCYC 1 cut(s) 3674
ErhI CCWWGG 7 cut(s) 861, 1517, 2643, 2735, 4292, 4543, 4613
Esp3I CGTCTC 3 cut(s) 1054, 2391, 3050
FalI AAGNNNNNCTT 2 cut(s) 1823, 1855
FaqI GGGAC 2 cut(s) 2246, 3804
FauI CCCGC 2 cut(s) 307, 993
FauNDI CATATG 1 cut(s) 1801
FbaI TGATCA 2 cut(s) 2692, 4927
FblI GTMKAC 1 cut(s) 4995
FriOI GRGCYC 1 cut(s) 3674
FspBI CTAG 5 cut(s) 1650, 2229, 3695, 4254, 4614
GlaI GCGC 6 cut(s) 74, 378, 448, 1756, 2789, 2907
GsaI CCCAGC 3 cut(s) 386, 762, 3826
GsuI CTGGAG 6 cut(s) 312, 1214, 1788, 2544, 3767, 4130
HaeII RGCGCY 1 cut(s) 2791
HapII CCGG 8 cut(s) 214, 252, 278, 443, 3201, 3544, 4212, 4533
HgaI GACGC 4 cut(s) 247, 2601, 2639, 2912
HhaI GCGC 6 cut(s) 75, 379, 449, 1757, 2790, 2908
Hin1I GRCGYC 2 cut(s) 239, 2923
Hin6I GCGC 6 cut(s) 73, 377, 447, 1755, 2788, 2906
HinP1I GCGC 6 cut(s) 73, 377, 447, 1755, 2788, 2906
HincII GTYRAC 3 cut(s) 2656, 4128, 4996
HindII GTYRAC 3 cut(s) 2656, 4128, 4996
HindIII AAGCTT 4 cut(s) 114, 1629, 3068, 4415
HpaII CCGG 8 cut(s) 214, 252, 278, 443, 3201, 3544, 4212, 4533
Hpy166II GTNNAC 8 cut(s) 653, 1190, 2276, 2656, 4128, 4526, 4691, 4996
Hpy8I GTNNAC 8 cut(s) 653, 1190, 2276, 2656, 4128, 4526, 4691, 4996
Hpy99I CGWCG 5 cut(s) 142, 241, 250, 3064, 5000
HpyCH4IV ACGT 7 cut(s) 1030, 2278, 2860, 3982, 4246, 4687, 4839
HpySE526I ACGT 7 cut(s) 1030, 2278, 2860, 3982, 4246, 4687, 4839
Hsp92I GRCGYC 2 cut(s) 239, 2923
HspAI GCGC 6 cut(s) 73, 377, 447, 1755, 2788, 2906
KpnI GGTACC 2 cut(s) 4301, 5136
Ksp22I TGATCA 2 cut(s) 2692, 4927
LguI GCTCTTC 2 cut(s) 1068, 4071
MaeI CTAG 5 cut(s) 1650, 2229, 3695, 4254, 4614
MaeII ACGT 7 cut(s) 1030, 2278, 2860, 3982, 4246, 4687, 4839
MfeI CAATTG 2 cut(s) 3687, 4593
MflI RGATCY 9 cut(s) 175, 1685, 2179, 2428, 3366, 3742, 4427, 4461, 5138
MhlI GDGCHC 5 cut(s) 729, 883, 2284, 3674, 5032
MlsI TGGCCA 4 cut(s) 505, 1603, 1858, 4542
MluNI TGGCCA 4 cut(s) 505, 1603, 1858, 4542
MlyI GAGTC 8 cut(s) 79, 152, 266, 1232, 2065, 2228, 3647, 4375
MmeI TCCRAC 6 cut(s) 78, 120, 3374, 3645, 4143, 4445
Mox20I TGGCCA 4 cut(s) 505, 1603, 1858, 4542
Mph1103I ATGCAT 1 cut(s) 4912
MroXI GAANNNNTTC 5 cut(s) 1623, 2340, 2593, 4065, 4140
MscI TGGCCA 4 cut(s) 505, 1603, 1858, 4542
MslI CAYNNNNRTG 9 cut(s) 645, 714, 876, 978, 1641, 1852, 2948, 4935, 4941
Msp20I TGGCCA 4 cut(s) 505, 1603, 1858, 4542
MspA1I CMGCKG 4 cut(s) 125, 382, 4133, 4313
MspI CCGG 8 cut(s) 214, 252, 278, 443, 3201, 3544, 4212, 4533
MunI CAATTG 2 cut(s) 3687, 4593
Mva1269I GAATGC 2 cut(s) 1364, 3535
MvaI CCWGG 7 cut(s) 1364, 1767, 2076, 2562, 2727, 3354, 4148
NciI CCSGG 4 cut(s) 253, 444, 3201, 3545
NcoI CCATGG 4 cut(s) 861, 2735, 4292, 4543
NdeI CATATG 1 cut(s) 1801
NlaIV GGNNCC 6 cut(s) 457, 1401, 2430, 4299, 4783, 5134
NmuCI GTSAC 9 cut(s) 774, 794, 1775, 2507, 2578, 3248, 4378, 4979, 5159
NsiI ATGCAT 1 cut(s) 4912
NspI RCATGY 4 cut(s) 2942, 3935, 4363, 4940
NspV TTCGAA 1 cut(s) 2842
OliI CACNNNNGTG 1 cut(s) 978
PaeR7I CTCGAG 3 cut(s) 354, 2852, 3988
PagI TCATGA 5 cut(s) 937, 1636, 1939, 2689, 4437
PciI ACATGT 2 cut(s) 2938, 3931
PciSI GCTCTTC 2 cut(s) 1068, 4071
PcsI WCGNNNNNNNCGW 4 cut(s) 245, 1620, 4573, 4699
PctI GAATGC 2 cut(s) 1364, 3535
PdmI GAANNNNTTC 5 cut(s) 1623, 2340, 2593, 4065, 4140
PflFI GACNNNGTC 1 cut(s) 4793
PflMI CCANNNNNTGG 2 cut(s) 3829, 4153
PfoI TCCNGGA 2 cut(s) 1765, 2560
PinAI ACCGGT 1 cut(s) 4211
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 8 cut(s) 79, 151, 266, 1231, 2065, 2228, 3647, 4375
PmaCI CACGTG 1 cut(s) 4688
PmlI CACGTG 1 cut(s) 4688
PpsI GAGTC 8 cut(s) 79, 151, 266, 1231, 2065, 2228, 3647, 4375
Ppu21I YACGTR 4 cut(s) 1031, 2279, 3983, 4688
PscI ACATGT 2 cut(s) 2938, 3931
PshBI ATTAAT 2 cut(s) 891, 2810
PsiI TTATAA 1 cut(s) 4803
Psp124BI GAGCTC 1 cut(s) 3674
Psp1406I AACGTT 1 cut(s) 2860
Psp6I CCWGG 7 cut(s) 1362, 1765, 2074, 2560, 2725, 3352, 4146
PspCI CACGTG 1 cut(s) 4688
PspEI GGTNACC 2 cut(s) 794, 1775
PspFI CCCAGC 3 cut(s) 382, 758, 3822
PspGI CCWGG 7 cut(s) 1362, 1765, 2074, 2560, 2725, 3352, 4146
PspN4I GGNNCC 6 cut(s) 457, 1401, 2430, 4299, 4783, 5134
PspPI GGNCC 7 cut(s) 226, 407, 1898, 2128, 3173, 4080, 4781
PspXI VCTCGAGB 2 cut(s) 354, 3988
PstI CTGCAG 1 cut(s) 2665
PstNI CAGNNNCTG 5 cut(s) 2992, 3829, 4153, 4316, 4375
PsuI RGATCY 9 cut(s) 175, 1685, 2179, 2428, 3366, 3742, 4427, 4461, 5138
PsyI GACNNNGTC 1 cut(s) 4793
PvuI CGATCG 1 cut(s) 187
PvuII CAGCTG 4 cut(s) 125, 382, 4133, 4313
RseI CAYNNNNRTG 9 cut(s) 645, 714, 876, 978, 1641, 1852, 2948, 4935, 4941
SacI GAGCTC 1 cut(s) 3674
SalI GTCGAC 1 cut(s) 4994
SapI GCTCTTC 2 cut(s) 1068, 4071
Sau96I GGNCC 7 cut(s) 226, 407, 1898, 2128, 3173, 4080, 4781
SbfI CCTGCAGG 1 cut(s) 2665
ScaI AGTACT 4 cut(s) 2319, 2765, 3164, 4665
SchI GAGTC 8 cut(s) 79, 152, 266, 1232, 2065, 2228, 3647, 4375
SdaI CCTGCAGG 1 cut(s) 2665
SduI GDGCHC 5 cut(s) 729, 883, 2284, 3674, 5032
SfcI CTRYAG 6 cut(s) 194, 2287, 2661, 3138, 4364, 5155
Sfr274I CTCGAG 3 cut(s) 354, 2852, 3988
SfuI TTCGAA 1 cut(s) 2842
SinI GGWCC 4 cut(s) 226, 1898, 3173, 4080
SlaI CTCGAG 3 cut(s) 354, 2852, 3988
SmiMI CAYNNNNRTG 9 cut(s) 645, 714, 876, 978, 1641, 1852, 2948, 4935, 4941
SmlI CTYRAG 5 cut(s) 107, 354, 2114, 2852, 3988
SmoI CTYRAG 5 cut(s) 107, 354, 2114, 2852, 3988
SnaBI TACGTA 1 cut(s) 1031
Sse8387I CCTGCAGG 1 cut(s) 2665
SspI AATATT 6 cut(s) 856, 1866, 2349, 3501, 3786, 4187
SspMI CTAG 5 cut(s) 1650, 2229, 3695, 4254, 4614
SstI GAGCTC 1 cut(s) 3674
StyI CCWWGG 7 cut(s) 861, 1517, 2643, 2735, 4292, 4543, 4613
TaiI ACGT 7 cut(s) 1033, 2281, 2863, 3985, 4249, 4690, 4842
TaqII GACCGA 1 cut(s) 4097
TatI WGTACW 6 cut(s) 1466, 2317, 2763, 3162, 4651, 4663
TauI GCSGC 7 cut(s) 244, 262, 328, 331, 334, 1873, 4349
TseFI GTSAC 9 cut(s) 774, 794, 1775, 2507, 2578, 3248, 4378, 4979, 5159
Tsp45I GTSAC 9 cut(s) 774, 794, 1775, 2507, 2578, 3248, 4378, 4979, 5159
TspGWI ACGGA 4 cut(s) 423, 451, 2526, 3064
Tth111I GACNNNGTC 1 cut(s) 4793
Van91I CCANNNNNTGG 2 cut(s) 3829, 4153
VpaK11BI GGWCC 4 cut(s) 226, 1898, 3173, 4080
VspI ATTAAT 2 cut(s) 891, 2810
XagI CCTNNNNNAGG 2 cut(s) 3138, 4145
XapI RAATTY 9 cut(s) 1208, 1619, 2340, 2482, 2589, 2833, 3855, 3964, 4922
XceI RCATGY 4 cut(s) 2942, 3935, 4363, 4940
XcmI CCANNNNNNNNNTGG 1 cut(s) 3899
XhoI CTCGAG 3 cut(s) 354, 2852, 3988
XmaJI CCTAGG 1 cut(s) 4613
XmiI GTMKAC 1 cut(s) 4995
XmnI GAANNNNTTC 5 cut(s) 1623, 2340, 2593, 4065, 4140
XspI CTAG 5 cut(s) 1650, 2229, 3695, 4254, 4614
ZrmI AGTACT 4 cut(s) 2319, 2765, 3164, 4665
Zsp2I ATGCAT 1 cut(s) 4912
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.