Rroxscaffold_2G00116210

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002907
Physical Location & Seq
Forward (+)
43265263 .. 43280139
14877 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00116210.1

Sequence Viewer

Length: 5142 bp
ATGGAGACTGGCTCGGAGGGAGAGAAGACGAGAGCTCAGGCTCAGGAAGGTGAGATCAGAACCAAGCGCAAAATGAAGACTCGTTCGCAGTTGGAAGTTCTTGAGCAAGCTTATGCAGCTGAGCAATACCCGTCGGAGTCGGTGAGAGCTGAGCTGTCGGCGAAATTGGATCTTTCCGATCGGCAACTACAGATGTGGTTTTGCCACCGGAGATTGAAGGACCGTAAGCCGACGCCGACCACCGGAAAGCGGCAGAGGAATGACTCTCCGGCTGGAACGACTGGAGTGGTTTCTGAGGTAATGGCGGGGAGTGAGCCGCCGCCGCAGCAGTTGATAATAGATGCTCGAGCTGTTGATTTTGTGGAGGCGCAGCTGGGGGAGCCATTCAGGGAGGATGGGCCAATACTGGGCTTGGAGTTTGATCCGTTGCCACCGGGTGCATTTGGTTCCTCCGTAGGAGTGGCAACTGTTGGGCAGCAGAAGCAATCTGGATGGCCATATGATGCAAATATATATGAGCAGTCTGATGCTAAACCAATTAAGGGTGCTTCAAGGACTGTCCATGAATACCAGTTTCTTCCAGAAAAGCCAACTGTTAGGACAGACACTTATGAAAGGGCTGCCCCATCTCACCGCTATGGTTCACCCACTGAATCTCGCCATGCAAGGTTTCCATTATCTACTGTGTCCTCGTTCATTCATGGTAATGAACAAGTACCCACTGTATATGGATTTCAAAGCCAGATGGCTGGACTAAATCTTTTGCCACAGCAAGGCAGGGCTGCTCACCTCTTGCCATCTGCTTCAGAAGAATATGGCAATGTTCATGGAAAGAACTTCACGAATATTACCATGGATGCTTATTTTAGTGCTCACCCGATTAATCAACTGAACAATCCTTTTATACCATTTGCCAGAAGGGTGGCTCATGACGAGGATGATGTTCTAAGATTGGAAAGGAAGAACAAGAGTGAAGAAGCAAGAATAATGCGGGAAGTTGAAGCCCATGAGAAAAGGATACGTAAAGAGCTTGAAAAACAAGATATCCTGAGACGAAAGAGAGAGGAGCAGATAAGGAAAGAAATGGAGAGGATTGATCGGGAGAGGAGGAAGGAAGAGGAAAGGCTATTGCGTGAAAAGCAGAGAGAGGAGGAGAGATACCTAAGGGAGCAAAAGCGTGAACTGGAACGAAAGGAGAAATTCTTACAGAAGGAGTCAATAAGAGCTGAGAAAATGAGACAAAAAGAAGAATTACGCAGAGAGAAAGAGGCAGCAAGGCTCAAAGCTGCTAATGAGAGAGCTATTGCTCGTAAAAATGCTAAAGAATCAATGGAACTCATTGAGGATGAATGCTTGGAACTCATGGAGTTAGCTGCATCAAGCAAGGGACTTCCTTCCATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGATATGCAGAGTACATTCCCACCCAAATCTGTGCAGTTGAAAAAACCTTTTGGTATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGGCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAACACCCTCTATGGGGGTGGGAGCTAATCAGAACTGCACTGCAAATCCTGGGGGCGGTCACCCTCAGATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACTTAAATCCACTGACATGGCCAGAAATATTGCGGCAATTTTCTGTATCAGCAGGATTTGGACCCCAACTGAAGAAGAGGGGTGTTGAACCTCTTTATCTTCATGAGGACAATGAGTTGAAGGCTGAGGGTAATGATGTCAAGGATATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTTGCTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGCAAATCAAGGCATCGCTTGACTCCTGGAACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGGACTGCTCCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACATGTGATTCCGAGGCAGTACTTTCTGCAGCCAGGGAAAGAATCCAATTATTCAATAGTAGGATTTTTGATGTAGAAGGAGCTGATGAAGCTGAAAGAGATGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGGTTGATGATTTAGGCACTGAAATAAATCCAAAGATAGTAGATCAGAATTCTGAAGAAGCAAAAAAAGTCAGTGACAGAATATCTTTGGAAAGTAGAAAAGGAAGTTACGAGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAGAAGGAATTCCTTTAATCAATTCTGAAGCGTTCGTCAAGGTTGAAGACACTGGTTGTTCCTTGGATGATTCTGTTGACGCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAATCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCTTTAATTGGTGTGGCAATAGAAGGAAACTCTATTCGAATTGTTCTTGAGGAACGTTTAGAAGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGTGCAAGTTGATAAACGGCGTCTGAAAGAAGAACATGTCATCAAAATGCAATATACCCCTTTCATTGGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCTTCAGAAGATAGGCAAAGTCCATCTCCTACTGTTGATGAGAAAAACATTGAGACGACCGTGAAGCTTTCCGTCCTGCAAGAGCAGATGGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCTTCTGCAGGAAACCTACAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTGTTGCTGATAAGTCACACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCACTGCCCCTTGGTCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGTAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGTGCGTGGGTATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTCAAGGAAACTGTTAAAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATCAAAGACACTGCTAAAGCAGAAGATGTTGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACAGATAGCCCTACCAGCTCCGTTTCTGTTGCTGACTCTGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAACAAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGATCTGGAGAGGTGGATTTGGAAAGAATGTCTTGGTTCTTCAATATTATGTGCCGACAAAAAAGGGAAAAAAAGGTGTCCCCAGCTGCTGGACATCTGTAATTCTTGTCGTGAAATTTTTTATTTTGAAGAGGATCACTGTCATTCTTGCCATAGAACTTTTGGTAAGGGCGAAATAGATTTTTCACAACATGTGGTGCTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGACTTCTTCTCCCCTCAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAAATCTTGGGCTAGAAAGTTACATTTCTCATCATCAGCTGAAGACCTTCTCCAGGTTCTGGCATCTCTGGAGAGTGCTGTAAAGATAGAATATTTATCTTCAAACTTTGAGACTACTGGTGAGCTGCTATATACCTCAAATGAAAAAGGATGTGGTACTAGCGGTTCTTCTTCCCCTGATAGCGTTGCTGTGCTTCCATGGATACCACTTACAACAGCTGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCAGCCATCTCAAACATGCTACAGCAGAAGCTGGAGACTCAGAAGGACAAGGAATGTGGGGATTTCATAAAGCTTCCAACAAGATCTGCTGTCATGAAAAGTTCCCTGAATGAGGAGATCTCAGGAACTCCACATCAATCTGAAGATTCTAAAGAAGGCCATTGGGAGTATCTGGGGAGTGGACTTACTGGCTCTGGCCATGGAAGAGGCCGTAGTCGAACTCGTGCTGGGCGAGTGCCAAAAAAGGCAATTGGTTTGAGGCGCAATCCTAGGAAGAAGAGTAGAGTTACAGCCAAAGATAGATTAGGACAGGTGCAAGTACTGAAATGGAAAGGCAGACCACGTGGACGAGGAGGGCGAAAGCGTGGAAATCGGAGCATTAGAGGCAAGGAGACAATGTTTAACAGGATAATTAAGATTGGTGGGGATAGAACTGGCCCCAAAGACAGTGTCTATTATAAATCACCAGAGGACTTGGTTGAGGAGAATTGGAATGTAGGTGAAACTGCTGCAGAAAATGCAAGCGGTTCTGGAAGGTCAGAGTATGAAGTGGAAAATGGGGATGCGTCAGGAGATGAATTTGATCATGTGCATGTGGATGACTATGCCGGTGGCTTCAATGGCAAGTCTGATGACTTATTGGAGGGAGGCAAATATAACGTGGAGGGTGATGAAGATGATGTCGACGATGAGGTGGATGACGATGAAGGTGAAGGAGCACAAGATTCAAAAGGGGGGATTTTGATGGTGCAAGATATATCAATGGAGAGTGGGATGAAGAAGAACACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1713

Amino Acids

192.75

Weight (kDa)

5.66

Isoelectric Point (pI)

50.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 20 - 76 2.4e-16 Homeodomain
DDT PF02791 509 - 564 1.3e-18 DDT domain
HARE-HTH PF05066 694 - 761 1.2e-17 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 911 - 954 3.9e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1094 - 1167 1.7e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4809
AccB7I CCANNNNNTGG 2 cut(s) 3835, 4159
AccI GTMKAC 1 cut(s) 5034
AclI AACGTT 1 cut(s) 2866
AcoI YGGCCR 4 cut(s) 494, 1592, 1847, 4546
AcsI RAATTY 7 cut(s) 1199, 1610, 2485, 2592, 3861, 3970, 4928
AcvI CACGTG 1 cut(s) 4694
AcyI GRCGYC 2 cut(s) 233, 2929
AdeI CACNNNGTG 1 cut(s) 437
AfaI GTAC 9 cut(s) 717, 1459, 2280, 2322, 2771, 3170, 3625, 4258, 4671
AflIII ACRYGT 3 cut(s) 2303, 2944, 3937
AjnI CCWGG 7 cut(s) 1756, 2077, 2333, 2563, 2731, 3358, 4152
Alw21I GWGCWC 5 cut(s) 37, 874, 2287, 3680, 5071
Alw26I GTCTC 8 cut(s) 1045, 1231, 1410, 2562, 3056, 4205, 4379, 4736
AlwNI CAGNNNCTG 5 cut(s) 2998, 3835, 4159, 4322, 4381
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 9 cut(s) 398, 494, 1592, 1847, 2131, 4507, 4546, 4558, 4786
ApoI RAATTY 7 cut(s) 1199, 1610, 2485, 2592, 3861, 3970, 4928
ArsI GACNNNNNNTTYG 2 cut(s) 4785, 4817
AseI ATTAAT 1 cut(s) 882
Asp700I GAANNNNTTC 4 cut(s) 1614, 2592, 4071, 4146
AspA2I CCTAGG 1 cut(s) 4619
AspLEI GCGC 4 cut(s) 69, 370, 2796, 4614
AspS9I GGNCC 7 cut(s) 220, 398, 1889, 2131, 3179, 4086, 4787
AsuC2I CCSGG 3 cut(s) 435, 3207, 3551
AsuII TTCGAA 1 cut(s) 2848
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 4 cut(s) 220, 1889, 3179, 4086
AvrII CCTAGG 1 cut(s) 4619
AxyI CCTNAGG 4 cut(s) 1163, 2436, 2573, 4010
BalI TGGCCA 4 cut(s) 496, 1594, 1849, 4548
BamHI GGATCC 1 cut(s) 2431
BanII GRGCYC 2 cut(s) 37, 3680
BauI CACGAG 1 cut(s) 4572
BbrPI CACGTG 1 cut(s) 4694
BbsI GAAGAC 5 cut(s) 32, 83, 2637, 3503, 4149
Bbv12I GWGCWC 5 cut(s) 37, 874, 2287, 3680, 5071
BbvCI CCTCAGC 1 cut(s) 1953
BceAI ACGGC 2 cut(s) 2942, 4545
BcgI CGANNNNNNTGC 2 cut(s) 3377, 3411
BciT130I CCWGG 7 cut(s) 1758, 2079, 2335, 2565, 2733, 3360, 4154
BciVI GTATCC 2 cut(s) 1011, 4296
BclI TGATCA 2 cut(s) 2698, 4933
BcnI CCSGG 3 cut(s) 435, 3207, 3551
BcoDI GTCTC 8 cut(s) 1045, 1231, 1410, 2562, 3056, 4205, 4379, 4736
BfaI CTAG 6 cut(s) 1641, 2232, 3701, 4113, 4260, 4620
BfmI CTRYAG 7 cut(s) 188, 2290, 2328, 2667, 3144, 4370, 4860
BfoI RGCGCY 1 cut(s) 2797
BfuI GTATCC 2 cut(s) 1011, 4296
BglII AGATCT 3 cut(s) 3748, 4433, 4467
BlnI CCTAGG 1 cut(s) 4619
BlpI GCTNAGC 2 cut(s) 120, 150
BmcAI AGTACT 4 cut(s) 2322, 2771, 3170, 4671
Bme18I GGWCC 4 cut(s) 220, 1889, 3179, 4086
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 7 cut(s) 220, 398, 1889, 2131, 3179, 4086, 4787
BmiI GGNNCC 5 cut(s) 381, 448, 1891, 2433, 4789
BmrI ACTGGG 1 cut(s) 416
BmuI ACTGGG 1 cut(s) 416
BpiI GAAGAC 5 cut(s) 32, 83, 2637, 3503, 4149
BplI GAGNNNNNCTC 5 cut(s) 28, 2245, 2277, 4454, 4486
BpmI CTGGAG 6 cut(s) 303, 2547, 3773, 4136, 4190, 4403
Bpu10I CCTNAGC 3 cut(s) 36, 42, 1953
Bpu1102I GCTNAGC 2 cut(s) 120, 150
Bpu14I TTCGAA 1 cut(s) 2848
BpuEI CTTGAG 3 cut(s) 122, 2138, 2879
BpuMI CCSGG 3 cut(s) 435, 3207, 3551
Bsa29I ATCGAT 1 cut(s) 3283
BsaAI YACGTR 4 cut(s) 1022, 2282, 3989, 4694
BsaBI GATNNNNATC 1 cut(s) 2727
BsaHI GRCGYC 2 cut(s) 233, 2929
BsaI GGTCTC 1 cut(s) 2562
BsaWI WCCGGW 2 cut(s) 207, 242
BsaXI ACNNNNNCTCC 6 cut(s) 1989, 2019, 3988, 4018, 4733, 4763
Bse118I RCCGGY 1 cut(s) 4958
Bse21I CCTNAGG 4 cut(s) 1163, 2436, 2573, 4010
Bse3DI GCAATG 1 cut(s) 826
Bse8I GATNNNNATC 1 cut(s) 2727
BseBI CCWGG 7 cut(s) 1758, 2079, 2335, 2565, 2733, 3360, 4154
BseCI ATCGAT 1 cut(s) 3283
BseJI GATNNNNATC 1 cut(s) 2727
BseMI GCAATG 1 cut(s) 826
BseYI CCCAGC 3 cut(s) 373, 3828, 4577
BsgI GTGCAG 2 cut(s) 1499, 1729
Bsh1285I CGRYCG 3 cut(s) 181, 3069, 3397
BshFI GGCC 9 cut(s) 400, 496, 1594, 1849, 2133, 4509, 4548, 4560, 4788
BshVI ATCGAT 1 cut(s) 3283
BsiEI CGRYCG 3 cut(s) 181, 3069, 3397
BsiHKAI GWGCWC 5 cut(s) 37, 874, 2287, 3680, 5071
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 7 cut(s) 208, 243, 269, 434, 3207, 3550, 4959
BslFI GGGAC 3 cut(s) 1401, 2249, 3810
BsmAI GTCTC 8 cut(s) 1045, 1231, 1410, 2562, 3056, 4205, 4379, 4736
BsmBI CGTCTC 2 cut(s) 1045, 3056
BsmFI GGGAC 3 cut(s) 1401, 2249, 3810
BsmI GAATGC 2 cut(s) 1355, 3541
BsnI GGCC 9 cut(s) 400, 496, 1594, 1849, 2133, 4509, 4548, 4560, 4788
Bso31I GGTCTC 1 cut(s) 2562
BsoBI CYCGRG 1 cut(s) 345
Bsp119I TTCGAA 1 cut(s) 2848
Bsp1286I GDGCHC 5 cut(s) 37, 874, 2287, 3680, 5071
Bsp1720I GCTNAGC 2 cut(s) 120, 150
Bsp19I CCATGG 4 cut(s) 852, 2741, 4298, 4549
BspANI GGCC 9 cut(s) 400, 496, 1594, 1849, 2133, 4509, 4548, 4560, 4788
BspDI ATCGAT 1 cut(s) 3283
BspHI TCATGA 5 cut(s) 928, 1627, 1930, 2695, 4443
BspLI GGNNCC 5 cut(s) 381, 448, 1891, 2433, 4789
BspMAI CTGCAG 4 cut(s) 2332, 2671, 3148, 4864
BspQI GCTCTTC 1 cut(s) 4077
BspT104I TTCGAA 1 cut(s) 2848
BspTNI GGTCTC 1 cut(s) 2562
BsrDI GCAATG 1 cut(s) 826
BsrFI RCCGGY 1 cut(s) 4958
BssAI RCCGGY 1 cut(s) 4958
BssNI GRCGYC 2 cut(s) 233, 2929
BssSI CACGAG 1 cut(s) 4572
BssT1I CCWWGG 8 cut(s) 852, 1508, 2646, 2741, 3295, 4298, 4549, 4619
Bst2BI CACGAG 1 cut(s) 4572
Bst2UI CCWGG 7 cut(s) 1758, 2079, 2335, 2565, 2733, 3360, 4154
Bst6I CTCTTC 7 cut(s) 1110, 1898, 3414, 3870, 4077, 4549, 4622
BstACI GRCGYC 2 cut(s) 233, 2929
BstAPI GCANNNNNTGC 2 cut(s) 2810, 4868
BstBAI YACGTR 4 cut(s) 1022, 2282, 3989, 4694
BstBI TTCGAA 1 cut(s) 2848
BstC8I GCNNGC 2 cut(s) 108, 4873
BstDSI CCRYGG 4 cut(s) 852, 2741, 4298, 4549
BstEII GGTNACC 1 cut(s) 1766
BstENI CCTNNNNNAGG 4 cut(s) 3144, 3299, 4151, 4460
BstH2I RGCGCY 1 cut(s) 2797
BstHHI GCGC 4 cut(s) 69, 370, 2796, 4614
BstMAI GTCTC 8 cut(s) 1045, 1231, 1410, 2562, 3056, 4205, 4379, 4736
BstMCI CGRYCG 3 cut(s) 181, 3069, 3397
BstNI CCWGG 7 cut(s) 1758, 2079, 2335, 2565, 2733, 3360, 4154
BstNSI RCATGY 5 cut(s) 2307, 2948, 3941, 4369, 4946
BstPI GGTNACC 1 cut(s) 1766
BstSFI CTRYAG 7 cut(s) 188, 2290, 2328, 2667, 3144, 4370, 4860
BstSNI TACGTA 1 cut(s) 1022
BstV2I GAAGAC 5 cut(s) 32, 83, 2637, 3503, 4149
BstX2I RGATCY 8 cut(s) 169, 1676, 2182, 2431, 3372, 3748, 4433, 4467
BstXI CCANNNNNNTGG 3 cut(s) 749, 922, 1845
BstYI RGATCY 8 cut(s) 169, 1676, 2182, 2431, 3372, 3748, 4433, 4467
Bsu15I ATCGAT 1 cut(s) 3283
Bsu36I CCTNAGG 4 cut(s) 1163, 2436, 2573, 4010
BsuI GTATCC 2 cut(s) 1011, 4296
BsuRI GGCC 9 cut(s) 400, 496, 1594, 1849, 2133, 4509, 4548, 4560, 4788
BsuTUI ATCGAT 1 cut(s) 3283
BtgI CCRYGG 4 cut(s) 852, 2741, 4298, 4549
BtgZI GCGATG 1 cut(s) 2051
BtsI GCAGTG 6 cut(s) 1746, 1999, 2913, 3287, 3570, 3622
Cac8I GCNNGC 2 cut(s) 108, 4873
CaiI CAGNNNCTG 5 cut(s) 2998, 3835, 4159, 4322, 4381
CciI TCATGA 5 cut(s) 928, 1627, 1930, 2695, 4443
CfoI GCGC 4 cut(s) 69, 370, 2796, 4614
Cfr10I RCCGGY 1 cut(s) 4958
Cfr13I GGNCC 7 cut(s) 220, 398, 1889, 2131, 3179, 4086, 4787
ClaI ATCGAT 1 cut(s) 3283
CseI GACGC 4 cut(s) 241, 2672, 2918, 4905
Csp6I GTAC 9 cut(s) 716, 1458, 2279, 2321, 2770, 3169, 3624, 4257, 4670
CspCI CAANNNNNGTGG 2 cut(s) 4387, 4422
CviQI GTAC 9 cut(s) 716, 1458, 2279, 2321, 2770, 3169, 3624, 4257, 4670
DraI TTTAAA 2 cut(s) 2889, 3735
DraIII CACNNNGTG 1 cut(s) 437
EaeI YGGCCR 4 cut(s) 494, 1592, 1847, 4546
Eam1104I CTCTTC 7 cut(s) 1110, 1898, 3414, 3870, 4077, 4549, 4622
EarI CTCTTC 7 cut(s) 1110, 1898, 3414, 3870, 4077, 4549, 4622
Ecl136II GAGCTC 2 cut(s) 35, 3678
Eco105I TACGTA 1 cut(s) 1022
Eco130I CCWWGG 8 cut(s) 852, 1508, 2646, 2741, 3295, 4298, 4549, 4619
Eco24I GRGCYC 2 cut(s) 37, 3680
Eco31I GGTCTC 1 cut(s) 2562
Eco32I GATATC 2 cut(s) 1045, 3743
Eco47I GGWCC 4 cut(s) 220, 1889, 3179, 4086
Eco53kI GAGCTC 2 cut(s) 35, 3678
Eco72I CACGTG 1 cut(s) 4694
Eco81I CCTNAGG 4 cut(s) 1163, 2436, 2573, 4010
Eco88I CYCGRG 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 1766
EcoICRI GAGCTC 2 cut(s) 35, 3678
EcoNI CCTNNNNNAGG 4 cut(s) 3144, 3299, 4151, 4460
EcoO109I RGGNCCY 1 cut(s) 2131
EcoO65I GGTNACC 1 cut(s) 1766
EcoRI GAATTC 4 cut(s) 1610, 2485, 2592, 3970
EcoRII CCWGG 7 cut(s) 1756, 2077, 2333, 2563, 2731, 3358, 4152
EcoRV GATATC 2 cut(s) 1045, 3743
EcoT14I CCWWGG 8 cut(s) 852, 1508, 2646, 2741, 3295, 4298, 4549, 4619
EcoT38I GRGCYC 2 cut(s) 37, 3680
ErhI CCWWGG 8 cut(s) 852, 1508, 2646, 2741, 3295, 4298, 4549, 4619
Esp3I CGTCTC 2 cut(s) 1045, 3056
FalI AAGNNNNNCTT 4 cut(s) 1814, 1846, 3762, 3794
FaqI GGGAC 3 cut(s) 1401, 2249, 3810
FauI CCCGC 2 cut(s) 298, 984
FauNDI CATATG 2 cut(s) 499, 1792
FbaI TGATCA 2 cut(s) 2698, 4933
FblI GTMKAC 1 cut(s) 5034
FriOI GRGCYC 2 cut(s) 37, 3680
FspBI CTAG 6 cut(s) 1641, 2232, 3701, 4113, 4260, 4620
GlaI GCGC 4 cut(s) 68, 369, 2795, 4613
GsaI CCCAGC 3 cut(s) 377, 3832, 4581
GsuI CTGGAG 6 cut(s) 303, 2547, 3773, 4136, 4190, 4403
HaeII RGCGCY 1 cut(s) 2797
HaeIII GGCC 9 cut(s) 400, 496, 1594, 1849, 2133, 4509, 4548, 4560, 4788
HapII CCGG 7 cut(s) 208, 243, 269, 434, 3207, 3550, 4959
HgaI GACGC 4 cut(s) 241, 2672, 2918, 4905
HhaI GCGC 4 cut(s) 69, 370, 2796, 4614
Hin1I GRCGYC 2 cut(s) 233, 2929
Hin6I GCGC 4 cut(s) 67, 368, 2794, 4612
HinP1I GCGC 4 cut(s) 67, 368, 2794, 4612
HincII GTYRAC 2 cut(s) 2662, 5035
HindII GTYRAC 2 cut(s) 2662, 5035
HindIII AAGCTT 4 cut(s) 108, 1620, 3074, 4421
HpaII CCGG 7 cut(s) 208, 243, 269, 434, 3207, 3550, 4959
Hpy166II GTNNAC 7 cut(s) 644, 1181, 2279, 2662, 4532, 4697, 5035
Hpy8I GTNNAC 7 cut(s) 644, 1181, 2279, 2662, 4532, 4697, 5035
Hpy99I CGWCG 3 cut(s) 136, 235, 5039
HpyCH4IV ACGT 6 cut(s) 1021, 2281, 2866, 3988, 4693, 5010
HpySE526I ACGT 6 cut(s) 1021, 2281, 2866, 3988, 4693, 5010
Hsp92I GRCGYC 2 cut(s) 233, 2929
HspAI GCGC 4 cut(s) 67, 368, 2794, 4612
Ksp22I TGATCA 2 cut(s) 2698, 4933
LguI GCTCTTC 1 cut(s) 4077
MaeI CTAG 6 cut(s) 1641, 2232, 3701, 4113, 4260, 4620
MaeII ACGT 6 cut(s) 1021, 2281, 2866, 3988, 4693, 5010
MaeIII GTNAC 8 cut(s) 1766, 2510, 2543, 2581, 3225, 3254, 4119, 4636
MfeI CAATTG 2 cut(s) 3693, 4599
MflI RGATCY 8 cut(s) 169, 1676, 2182, 2431, 3372, 3748, 4433, 4467
MhlI GDGCHC 5 cut(s) 37, 874, 2287, 3680, 5071
MlsI TGGCCA 4 cut(s) 496, 1594, 1849, 4548
MluNI TGGCCA 4 cut(s) 496, 1594, 1849, 4548
MlyI GAGTC 8 cut(s) 73, 146, 257, 1223, 2068, 2231, 3653, 4381
MmeI TCCRAC 5 cut(s) 72, 114, 3380, 3651, 4451
Mox20I TGGCCA 4 cut(s) 496, 1594, 1849, 4548
MroXI GAANNNNTTC 4 cut(s) 1614, 2592, 4071, 4146
MscI TGGCCA 4 cut(s) 496, 1594, 1849, 4548
MslI CAYNNNNRTG 7 cut(s) 636, 705, 1632, 1843, 2954, 4941, 4947
Msp20I TGGCCA 4 cut(s) 496, 1594, 1849, 4548
MspA1I CMGCKG 5 cut(s) 119, 373, 3832, 4139, 4319
MspI CCGG 7 cut(s) 208, 243, 269, 434, 3207, 3550, 4959
MunI CAATTG 2 cut(s) 3693, 4599
Mva1269I GAATGC 2 cut(s) 1355, 3541
MvaI CCWGG 7 cut(s) 1758, 2079, 2335, 2565, 2733, 3360, 4154
NciI CCSGG 3 cut(s) 435, 3207, 3551
NcoI CCATGG 4 cut(s) 852, 2741, 4298, 4549
NdeI CATATG 2 cut(s) 499, 1792
NlaIV GGNNCC 5 cut(s) 381, 448, 1891, 2433, 4789
NmuCI GTSAC 5 cut(s) 1766, 2510, 2581, 3225, 3254
NspI RCATGY 5 cut(s) 2307, 2948, 3941, 4369, 4946
NspV TTCGAA 1 cut(s) 2848
PaeR7I CTCGAG 1 cut(s) 345
PagI TCATGA 5 cut(s) 928, 1627, 1930, 2695, 4443
PciI ACATGT 3 cut(s) 2303, 2944, 3937
PciSI GCTCTTC 1 cut(s) 4077
PcsI WCGNNNNNNNCGW 3 cut(s) 1611, 4579, 4705
PctI GAATGC 2 cut(s) 1355, 3541
PdmI GAANNNNTTC 4 cut(s) 1614, 2592, 4071, 4146
PflFI GACNNNGTC 1 cut(s) 4799
PflMI CCANNNNNTGG 2 cut(s) 3835, 4159
PfoI TCCNGGA 2 cut(s) 2077, 2563
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 8 cut(s) 73, 145, 257, 1222, 2068, 2231, 3653, 4381
PmaCI CACGTG 1 cut(s) 4694
PmlI CACGTG 1 cut(s) 4694
PpsI GAGTC 8 cut(s) 73, 145, 257, 1222, 2068, 2231, 3653, 4381
Ppu21I YACGTR 4 cut(s) 1022, 2282, 3989, 4694
PscI ACATGT 3 cut(s) 2303, 2944, 3937
PshBI ATTAAT 1 cut(s) 882
PsiI TTATAA 1 cut(s) 4809
Psp124BI GAGCTC 2 cut(s) 37, 3680
Psp1406I AACGTT 1 cut(s) 2866
Psp6I CCWGG 7 cut(s) 1756, 2077, 2333, 2563, 2731, 3358, 4152
PspCI CACGTG 1 cut(s) 4694
PspEI GGTNACC 1 cut(s) 1766
PspFI CCCAGC 3 cut(s) 373, 3828, 4577
PspGI CCWGG 7 cut(s) 1756, 2077, 2333, 2563, 2731, 3358, 4152
PspN4I GGNNCC 5 cut(s) 381, 448, 1891, 2433, 4789
PspPI GGNCC 7 cut(s) 220, 398, 1889, 2131, 3179, 4086, 4787
PspXI VCTCGAGB 1 cut(s) 345
PsrI GAACNNNNNNTAC 2 cut(s) 4249, 4281
PstI CTGCAG 4 cut(s) 2332, 2671, 3148, 4864
PstNI CAGNNNCTG 5 cut(s) 2998, 3835, 4159, 4322, 4381
PsuI RGATCY 8 cut(s) 169, 1676, 2182, 2431, 3372, 3748, 4433, 4467
PsyI GACNNNGTC 1 cut(s) 4799
PvuI CGATCG 1 cut(s) 181
PvuII CAGCTG 5 cut(s) 119, 373, 3832, 4139, 4319
RsaI GTAC 9 cut(s) 717, 1459, 2280, 2322, 2771, 3170, 3625, 4258, 4671
RsaNI GTAC 9 cut(s) 716, 1458, 2279, 2321, 2770, 3169, 3624, 4257, 4670
RseI CAYNNNNRTG 7 cut(s) 636, 705, 1632, 1843, 2954, 4941, 4947
SacI GAGCTC 2 cut(s) 37, 3680
SalI GTCGAC 1 cut(s) 5033
SapI GCTCTTC 1 cut(s) 4077
Sau96I GGNCC 7 cut(s) 220, 398, 1889, 2131, 3179, 4086, 4787
ScaI AGTACT 4 cut(s) 2322, 2771, 3170, 4671
SchI GAGTC 8 cut(s) 73, 146, 257, 1223, 2068, 2231, 3653, 4381
SduI GDGCHC 5 cut(s) 37, 874, 2287, 3680, 5071
SfcI CTRYAG 7 cut(s) 188, 2290, 2328, 2667, 3144, 4370, 4860
Sfr274I CTCGAG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 2848
SinI GGWCC 4 cut(s) 220, 1889, 3179, 4086
SlaI CTCGAG 1 cut(s) 345
SmiMI CAYNNNNRTG 7 cut(s) 636, 705, 1632, 1843, 2954, 4941, 4947
SmlI CTYRAG 4 cut(s) 101, 345, 2117, 2858
SmoI CTYRAG 4 cut(s) 101, 345, 2117, 2858
SnaBI TACGTA 1 cut(s) 1022
SspI AATATT 5 cut(s) 847, 1857, 3507, 3792, 4193
SspMI CTAG 6 cut(s) 1641, 2232, 3701, 4113, 4260, 4620
SstI GAGCTC 2 cut(s) 37, 3680
StyI CCWWGG 8 cut(s) 852, 1508, 2646, 2741, 3295, 4298, 4549, 4619
TaiI ACGT 6 cut(s) 1024, 2284, 2869, 3991, 4696, 5013
TaqI TCGA 9 cut(s) 346, 1605, 2213, 2848, 3283, 3349, 3995, 4567, 5034
TaqII GACCGA 1 cut(s) 4103
TatI WGTACW 5 cut(s) 1457, 2320, 2769, 3168, 4669
TauI GCSGC 5 cut(s) 253, 319, 322, 325, 1864
TseFI GTSAC 5 cut(s) 1766, 2510, 2581, 3225, 3254
Tsp45I GTSAC 5 cut(s) 1766, 2510, 2581, 3225, 3254
TspGWI ACGGA 4 cut(s) 414, 442, 3070, 3634
Tth111I GACNNNGTC 1 cut(s) 4799
Van91I CCANNNNNTGG 2 cut(s) 3835, 4159
VpaK11BI GGWCC 4 cut(s) 220, 1889, 3179, 4086
VspI ATTAAT 1 cut(s) 882
XagI CCTNNNNNAGG 4 cut(s) 3144, 3299, 4151, 4460
XapI RAATTY 7 cut(s) 1199, 1610, 2485, 2592, 3861, 3970, 4928
XceI RCATGY 5 cut(s) 2307, 2948, 3941, 4369, 4946
XcmI CCANNNNNNNNNTGG 1 cut(s) 3905
XhoI CTCGAG 1 cut(s) 345
XmaJI CCTAGG 1 cut(s) 4619
XmiI GTMKAC 1 cut(s) 5034
XmnI GAANNNNTTC 4 cut(s) 1614, 2592, 4071, 4146
XspI CTAG 6 cut(s) 1641, 2232, 3701, 4113, 4260, 4620
ZrmI AGTACT 4 cut(s) 2322, 2771, 3170, 4671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.