FvH4_6g26700

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002907
Physical Location & Seq
Reverse (-)
20386457 .. 20401224
14768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g26700.t2

Sequence Viewer

Length: 5157 bp
ATGGAAACGGGCTCTGAGGGGGAGAAGACCAGAGCTCAGGCTCAGGCTCAGGAGATCAGGACCAAGCGGAAAATGAAGACTCGTTCGCAACTGGAAGCTCTTGAGAAAGCTTATGCTGTTGAGCAATACCCGTCGGAGTCGGTGAGAGCTGAGCTGTCGGCGAAATTGGATCTCTCCGATCGGCAGTTACAGATGTGGTTTTGCCACCGGAGGTTGAAGGACCGCAAGCCGACGCCGGCGACCGGAAAGCGGCAGAGGAATGACTCTCTGGCCGAAGCGACTGGAGTTGTTTCTGAGGAAGGACTACAGCAGTCGATCATAGAGGCGCGAGCTGTTGCATTTGTGGAGGCGCAGCTGGGCGAGCCGTTGAGGGAGGATGGGCCGATACTTGGGTTGGAGTTTGACTCGTTGCCGCCCGATGCATTTGGTTCGTCTATAGGAGTGGCAACTGTTAGGCAGCAAAAGCAATCAGGATGGCCATGTGATGCAAATATACACGAGCAGTCTAATGCTAAACCAATCAAGGGAGCTTCAAGGACTGTCCATGAATACCAGTTTCTTCCAGAAAAGCCAACTGTTAGGACAGATGCTTATGAAAGGGCTGCCCCGTCTCACCACTATGGTTCACCTACTGAATCTCGCCATCCAAGGATTCCATTATCAACTGTGTCCTCGTTCATTCATGGTAATGAACAAGTACCCACTGTATATGGATTTCAAAGCCCGATGGTCGGACTAAATCTTTTGTCACAGCAAGGCAGGGCGGGTCACCTCTTGCCATCTGCTGCAGAAGAATATGGCAATGTTCTTGGAAAAAACTTCACAAATGTTACCATGGATTCTCATTTTAGTACTCACCTGATTAATCAACTAAACAATCCTCTTATACCTTATGCCAGAAGGGTGGCTCATGACGAGGATGATGTTCTACAGTTGGAAAGGAAGCACAAGAGTGAAGAAGCAAGAATAATGCGGGAAGTTGAAGCCCATGAGAAAAGAATACGTAAAGAGCTTGAAAAACAAGATATCCTGAGACGAAAGAGAGAGGAGCAGATAAGGAAAGAAATGGAGAGGATTGATCGGGAGAGGAGGAAGGAAGAAGAAAGAATATTGCGTGAAAAGCAGAGAGAGGAGGAGAGATACCTAAGGGAGCAAAGGCGTGAACTGGAACGAAAGGAGAGATTCTTACAGAAGGAGTCAATAAGAGCTGAGAAAATGAGACAAAAAGAAGAATTACGAAGAGAGAAAGAGGCAGCAAGGCTTAAAGCTGCTAACGAGAGAGCTAGTGCTCGTAAAAATGCTAAAGAATCAACAGAACTCATTGAGGATGAATGCCTGGAACTCATGGAGTTAGCTGCATTGAGCAAGGGACTTCCTTCAATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGATATGCTGAGTACATTCCCACCCAAATCTGTGCAATTGAAAAAACCTTTTGGTATTCAACCTTGGATGGATTCAGATGAAAACATTGGAAATCTTCTTATGGCATGGAGATTTTTAATTAGTTTTGTGGATGTTCTTGGGCTCTGGCCGTTTACTCTCGATGAACTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGCTGGGCGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAGCACCTTCCATGGGGGTGGGAGCTAATCAGAACTGTGCTGCAAATCCCGGAGGCGGACACCCTCAGATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACTTAAATCCGCTGACATGGCCTGAAATATTGCGGCAATTTGCTGTATCAGCAGGATTTGGACCGCAACTGAAGAAGAGGGGTGTTGAACTAGCATATCGTCACGAGGACAATGAGGTTAGTGATGTCAAGGATATAATATCCAATTTACGCAGTGGAGTAGCTGTAAAGAATGCCTTCGCTATTATGCAAGAAAGGGGTTTTTCTAATCCTCGCAAATCAAGGCATCGCTTGACTCCCGGGACTGTTAAGTTTGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTACCATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTAACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTCTTTCTAGGGACTCGAAACTGTTTGAGAGGACTGCTCCTTCTACTTACTGTGTACGTGCTGCCTACAGAAAGGACACAACGGATTCCAAGGCAGTACTTTCTGCAGCCAGGGAAAGAATCCAAAATTTCAGAAGTAAGATTTTTGATGTAGAAGGAGCTGATGAAGCTGAAAGAGACGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGGTTGATGATTTAGGTACTGAAATAAGTTCAGAGATAGTAGCTCACAGATCTGAAGTAGCCAAAAAAGTGGGTGAGAAAATGTCTTTGGAAAGCAGAAAAGGACGTTACGAGGTTAATAAAGCTCCAGGAGACGTTAGGAATGTCACTGAAGGAGTTCCTTCAATCAATTCTGAAGCGTTCATCAAGGTTGAAGACACTGGTTCCTTGAATAATTCTGCTGACGCCACAGGGATCTGCACCAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAACCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGAGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTTCTGCTTTAATTGGTGTGGCTATAGAAGGAAACTCAATTCGAATTGTTCTTGAGGAACGTTTAGAGGCAGCGAATGCTTTAAAGAAGCAAATGTGGGCAGTGGTGCAAGTTGATAAACGGCGTTTCAAAGATGAACATTTCATCAAAATGCAATATACCCCTTTCAGTGGGAACAAGACCGAGCAGATCCCGACAATTCATTCTTCAGATGAGAGGCAAAGTCCATCTACTGTTGATGAGAAAAACATTGAGAGGACAGTGAAGCTTTCTATCCCACAAGACCATATTGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCCTCCGTGGGAAACCTACAAATGCAGGAGAACTCTGTTGGTCCAGACAATTACCCATTTCAGCAACCCAGTCTTATTGCTGATAAGTCACACAAACATTTAAAGTATTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCACTGCCTCTCGGCCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGCAGGATCTTTGTTGAGCTGCATGATGGTCGATGGAGGCTTATTGATTCTGAGGAGGGCTTTGATGGTCTTTTGGCATCTCTGGATGTGCGTGGGTATAGGGAATCCCATTTGCAGGCAATGTTGCAGATGACTGAAATATTCTTCAAAGAAACTGTTAGGAGAAACATGTTGCATTCTAACCGAAGGAGGCATGTCAAAGACACTTCTAAAATTGAAGCTGTAGAAATGCCTTCTGGGTATGATTGCAGTGGTGGTACGGATAGCCCTACCAGCTCTGTTTCTGTTGCTGACTTTGATATGTTAGAGAGCTCAATGACTTTCTCAATTGAGCTAGGAAAGGACGAAACTGAGAAAACTGGTGCTTTAAACAGATATCATGATCTGGAGAAGTGGATTTGGAAAGAATGTATTGGTTCTTCAATATTAAGTGCTGACAAAAATGGGAAAAAAAGGTGTCCGCAGCTGCTGGACATCTGTAATTCTTGTCGTGGAATTTTTTATTTTGAAGAAAATCACTGTCATTCTTGTCATAGAACTTTTGGCAAGGGCGAAGTAGTTTTTTCACAACATGTGGCATTGTGTAAAGAGAAGTTGAATTCTAATTGCTCGGCCTCTTCTCCGCTTAGGATGAGGTTGCTGAAGGTGCTATTTGCTTTGACTGAGGTTTATGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAATTCTTGGGGTAGAAAGTTACACTCCTCGTCATCAGCTGAAGAACTTCTCCAGGTTCTGGCATCACTGGAGAGTGCCATAAAGACAGAATATTTATCTTCAAATTTTGAGACTACCAGTGAGCTTCTATATTGCTCGAATGAAAAAGGATGTGTCACTAGCAATTTTTCTTCTCCTCATAGAGTTTCTGTGCTTCCATGGATACCACTCACAACCGCAGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCGGCCATCTCTAACATGCTACAGCAGAAGCTGGAGAGTCATAGAGAGAAGGAATCTGGGCATTTTATTAAGCTTCCAACAAGATCTGCTGTCATGAAAAGTTCCCAGAATAAGGAGAAGTCAGGAGCTCCACATCAATCTGAAGAATTCAAAGAAGGCCAGCAGGAGTATTTGAGGAGTGGACTTGCCAGCCCTTGCCATGGACTAGGTTGTGGTCAGACTCGTAGTGGGAGAGTGCCAAAAAAGGCAATTAGTTTGAGGGGCGATCCTAGGAAGAAGAGTAGAGTTACATCCAAAGATAGATTAGGACAGGTGCATGTACTGAAATGGAAAGGCAAACCACGTGCACAAGGAGGGCGTAAGCGTGGAAATCGGAGCATTAGAGGCAATCAGCCAACGCTAAACAGGATAATTCAGATTGGTGGGGAGAGAAATAGCCCAAAAGAGTGCGTCTTTTATAAACCAGCAGAGAACTTGGGTGAGGAGAACTGGAATGAAGGTGAAACTGCGGCAGAAAATGCAAGTGGTTCGGGAAGAACAGAGTATGAAGAGGAAAATGGCGATGCATCCAGAGATGAATTTGATCATGTGAATGTGGATGACTATGGCCGTGGCTTCAATGGCAAATCTGATGACTTATTGGAGGGAGGTGATTATAACGTGGAGGGTTACCAAGACGATGTCAATGAGGATTACGATGAAGGTGAAGCTGCACAAGATACAAAGGGGGATCCGGATTACGATGGTGCCAGATATATCAATGATGAGTGGGATGAAGAGGAATGCATAGCTGATGATGGACATCAGAACAGGATACTTCGGAACAGAAACAGATCCTCGTCGTCAGACTATAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1719

Amino Acids

194.34

Weight (kDa)

5.86

Isoelectric Point (pI)

51.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 20 - 76 1.4e-16 Homeodomain
DDT PF02791 503 - 558 2.5e-18 DDT domain
HARE-HTH PF05066 684 - 752 2.7e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 900 - 943 4e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1082 - 1153 6e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 4755, 4953
AccB1I GGYRCC 1 cut(s) 5042
AccB7I CCANNNNNTGG 1 cut(s) 4105
AccII CGCG 1 cut(s) 328
AccIII TCCGGA 1 cut(s) 5029
AclI AACGTT 1 cut(s) 2833
AcoI YGGCCR 6 cut(s) 270, 476, 1574, 3262, 4299, 4903
AcsI RAATTY 6 cut(s) 2320, 3825, 3928, 4150, 4442, 4874
AcuI CTGAAG 8 cut(s) 1901, 2481, 2577, 2601, 2964, 3992, 4107, 4458
AcvI CACGTG 1 cut(s) 4640
AcyI GRCGYC 2 cut(s) 233, 2631
AfaI GTAC 9 cut(s) 699, 853, 1441, 2250, 2292, 2425, 2738, 3589, 4617
AflIII ACRYGT 2 cut(s) 3498, 3901
AjnI CCWGG 7 cut(s) 1335, 2303, 2533, 2698, 3264, 3322, 4098
AjuI GAANNNNNNNTTGG 2 cut(s) 2477, 2509
AleI CACNNNNGTG 1 cut(s) 951
Alw21I GWGCWC 6 cut(s) 37, 1291, 1696, 3644, 4426, 4645
Alw26I GTCTC 7 cut(s) 615, 1027, 1213, 1392, 2364, 2532, 4151
Alw44I GTGCAC 1 cut(s) 4641
AlwNI CAGNNNCTG 3 cut(s) 3799, 4105, 4327
Ama87I CYCGRG 1 cut(s) 2048
Aor13HI TCCGGA 1 cut(s) 5029
ApaLI GTGCAC 1 cut(s) 4641
ApoI RAATTY 6 cut(s) 2320, 3825, 3928, 4150, 4442, 4874
AseI ATTAAT 1 cut(s) 864
Asp700I GAANNNNTTC 5 cut(s) 1596, 1985, 2562, 4017, 4092
AspA2I CCTAGG 1 cut(s) 4565
AspLEI GCGC 3 cut(s) 328, 352, 2763
AspS9I GGNCC 7 cut(s) 60, 220, 380, 1871, 2101, 3143, 4032
AsuC2I CCSGG 3 cut(s) 1740, 2049, 2050
AsuII TTCGAA 1 cut(s) 2815
AvaI CYCGRG 1 cut(s) 2048
AvaII GGWCC 5 cut(s) 60, 220, 1871, 3143, 4032
AvrII CCTAGG 1 cut(s) 4565
AxyI CCTNAGG 2 cut(s) 1145, 2406
BaeGI GKGCMC 1 cut(s) 4645
BaeI ACNNNNGTAYC 2 cut(s) 377, 410
BalI TGGCCA 1 cut(s) 478
BamHI GGATCC 2 cut(s) 2401, 5026
BanI GGYRCC 1 cut(s) 5042
BanII GRGCYC 5 cut(s) 14, 37, 1572, 3644, 4426
BarI GAAGNNNNNNTAC 2 cut(s) 3733, 3765
BauI CACGAG 2 cut(s) 497, 1913
BbrPI CACGTG 1 cut(s) 4640
BbsI GAAGAC 3 cut(s) 32, 83, 2607
Bbv12I GWGCWC 6 cut(s) 37, 1291, 1696, 3644, 4426, 4645
BceAI ACGGC 4 cut(s) 349, 1561, 2909, 4890
BcgI CGANNNNNNTGC 6 cut(s) 411, 445, 3341, 3375, 4806, 4840
BciT130I CCWGG 7 cut(s) 1337, 2305, 2535, 2700, 3266, 3324, 4100
BciVI GTATCC 2 cut(s) 4242, 5103
BclI TGATCA 2 cut(s) 2665, 4879
BcnI CCSGG 3 cut(s) 1740, 2049, 2050
BcoDI GTCTC 7 cut(s) 615, 1027, 1213, 1392, 2364, 2532, 4151
BfaI CTAG 8 cut(s) 1284, 1623, 1901, 2202, 3665, 4206, 4502, 4566
BfoI RGCGCY 1 cut(s) 2764
BfuI GTATCC 2 cut(s) 4242, 5103
BglII AGATCT 2 cut(s) 2456, 4379
BlnI CCTAGG 1 cut(s) 4565
BlpI GCTNAGC 1 cut(s) 150
BmcAI AGTACT 3 cut(s) 853, 2292, 2738
Bme18I GGWCC 5 cut(s) 60, 220, 1871, 3143, 4032
BmeT110I CYCGRG 1 cut(s) 2048
BmgT120I GGNCC 7 cut(s) 60, 220, 380, 1871, 2101, 3143, 4032
BmiI GGNNCC 4 cut(s) 2403, 2611, 5028, 5044
BmrI ACTGGG 1 cut(s) 3165
BmuI ACTGGG 1 cut(s) 3165
BpiI GAAGAC 3 cut(s) 32, 83, 2607
BplI GAGNNNNNCTC 4 cut(s) 389, 421, 2215, 2247
BpmI CTGGAG 6 cut(s) 303, 2517, 3737, 4082, 4136, 4349
Bpu10I CCTNAGC 4 cut(s) 36, 42, 48, 3956
Bpu1102I GCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 2815
BpuEI CTTGAG 3 cut(s) 122, 2108, 2846
BpuMI CCSGG 3 cut(s) 1740, 2049, 2050
Bsa29I ATCGAT 1 cut(s) 3247
BsaAI YACGTR 3 cut(s) 1004, 2252, 4640
BsaBI GATNNNNATC 1 cut(s) 5097
BsaHI GRCGYC 2 cut(s) 233, 2631
BsaWI WCCGGW 3 cut(s) 207, 242, 5029
BsaXI ACNNNNNCTCC 2 cut(s) 1959, 1989
Bse118I RCCGGY 1 cut(s) 235
Bse21I CCTNAGG 2 cut(s) 1145, 2406
Bse3DI GCAATG 2 cut(s) 808, 3456
Bse8I GATNNNNATC 1 cut(s) 5097
BseAI TCCGGA 1 cut(s) 5029
BseBI CCWGG 7 cut(s) 1337, 2305, 2535, 2700, 3266, 3324, 4100
BseCI ATCGAT 1 cut(s) 3247
BseJI GATNNNNATC 1 cut(s) 5097
BseMI GCAATG 2 cut(s) 808, 3456
BseSI GKGCMC 1 cut(s) 4645
BseYI CCCAGC 2 cut(s) 355, 1630
BsgI GTGCAG 2 cut(s) 2629, 4992
Bsh1236I CGCG 1 cut(s) 328
Bsh1285I CGRYCG 2 cut(s) 181, 243
BshNI GGYRCC 1 cut(s) 5042
BshVI ATCGAT 1 cut(s) 3247
BsiEI CGRYCG 2 cut(s) 181, 243
BsiHKAI GWGCWC 6 cut(s) 37, 1291, 1696, 3644, 4426, 4645
BsiHKCI CYCGRG 1 cut(s) 2048
BsiSI CCGG 6 cut(s) 208, 236, 243, 1740, 2049, 5030
BslFI GGGAC 3 cut(s) 1383, 2065, 2219
BsmAI GTCTC 7 cut(s) 615, 1027, 1213, 1392, 2364, 2532, 4151
BsmBI CGTCTC 4 cut(s) 615, 1027, 2364, 2532
BsmFI GGGAC 3 cut(s) 1383, 2065, 2219
BsmI GAATGC 5 cut(s) 1337, 1987, 2854, 3505, 5084
BsoBI CYCGRG 1 cut(s) 2048
Bsp119I TTCGAA 1 cut(s) 2815
Bsp1286I GDGCHC 8 cut(s) 14, 37, 1291, 1572, 1696, 3644, 4426, 4645
Bsp13I TCCGGA 1 cut(s) 5029
Bsp1720I GCTNAGC 1 cut(s) 150
Bsp19I CCATGG 5 cut(s) 834, 1701, 2708, 4244, 4495
BspDI ATCGAT 1 cut(s) 3247
BspEI TCCGGA 1 cut(s) 5029
BspFNI CGCG 1 cut(s) 328
BspHI TCATGA 5 cut(s) 910, 1609, 2662, 3709, 4389
BspLI GGNNCC 4 cut(s) 2403, 2611, 5028, 5044
BspMAI CTGCAG 2 cut(s) 790, 2302
BspQI GCTCTTC 1 cut(s) 4023
BspT104I TTCGAA 1 cut(s) 2815
BspT107I GGYRCC 1 cut(s) 5042
BsrDI GCAATG 2 cut(s) 808, 3456
BsrFI RCCGGY 1 cut(s) 235
BssAI RCCGGY 1 cut(s) 235
BssNI GRCGYC 2 cut(s) 233, 2631
BssSI CACGAG 2 cut(s) 497, 1913
BssT1I CCWWGG 9 cut(s) 647, 834, 1490, 1701, 2283, 2708, 4244, 4495, 4565
Bst2BI CACGAG 2 cut(s) 497, 1913
Bst2UI CCWGG 7 cut(s) 1337, 2305, 2535, 2700, 3266, 3324, 4100
Bst6I CTCTTC 7 cut(s) 1234, 1880, 3952, 4023, 4568, 4839, 5067
BstACI GRCGYC 2 cut(s) 233, 2631
BstAPI GCANNNNNTGC 3 cut(s) 2777, 2849, 4814
BstBAI YACGTR 3 cut(s) 1004, 2252, 4640
BstBI TTCGAA 1 cut(s) 2815
BstC8I GCNNGC 7 cut(s) 227, 237, 330, 362, 3447, 4457, 4486
BstDSI CCRYGG 7 cut(s) 834, 1701, 2708, 3108, 4244, 4495, 4906
BstEII GGTNACC 2 cut(s) 767, 4964
BstFNI CGCG 1 cut(s) 328
BstH2I RGCGCY 1 cut(s) 2764
BstHHI GCGC 3 cut(s) 328, 352, 2763
BstMAI GTCTC 7 cut(s) 615, 1027, 1213, 1392, 2364, 2532, 4151
BstMCI CGRYCG 2 cut(s) 181, 243
BstNI CCWGG 7 cut(s) 1337, 2305, 2535, 2700, 3266, 3324, 4100
BstNSI RCATGY 5 cut(s) 3502, 3527, 3905, 4315, 4616
BstPI GGTNACC 2 cut(s) 767, 4964
BstSLI GKGCMC 1 cut(s) 4645
BstSNI TACGTA 1 cut(s) 1004
BstUI CGCG 1 cut(s) 328
BstV2I GAAGAC 3 cut(s) 32, 83, 2607
BstXI CCANNNNNNTGG 3 cut(s) 904, 1708, 2476
Bsu15I ATCGAT 1 cut(s) 3247
Bsu36I CCTNAGG 2 cut(s) 1145, 2406
BsuI GTATCC 2 cut(s) 4242, 5103
BsuTUI ATCGAT 1 cut(s) 3247
BtgI CCRYGG 7 cut(s) 834, 1701, 2708, 3108, 4244, 4495, 4906
BtgZI GCGATG 2 cut(s) 2021, 4872
BtsI GCAGTG 4 cut(s) 1969, 2880, 3251, 3586
Cac8I GCNNGC 7 cut(s) 227, 237, 330, 362, 3447, 4457, 4486
CaiI CAGNNNCTG 3 cut(s) 3799, 4105, 4327
CciI TCATGA 5 cut(s) 910, 1609, 2662, 3709, 4389
CfoI GCGC 3 cut(s) 328, 352, 2763
Cfr10I RCCGGY 1 cut(s) 235
Cfr13I GGNCC 7 cut(s) 60, 220, 380, 1871, 2101, 3143, 4032
Cfr9I CCCGGG 1 cut(s) 2048
ClaI ATCGAT 1 cut(s) 3247
CseI GACGC 3 cut(s) 241, 2639, 4735
Csp6I GTAC 9 cut(s) 698, 852, 1440, 2249, 2291, 2424, 2737, 3588, 4616
CviQI GTAC 9 cut(s) 698, 852, 1440, 2249, 2291, 2424, 2737, 3588, 4616
DraI TTTAAA 3 cut(s) 2856, 3204, 3699
EaeI YGGCCR 6 cut(s) 270, 476, 1574, 3262, 4299, 4903
Eam1104I CTCTTC 7 cut(s) 1234, 1880, 3952, 4023, 4568, 4839, 5067
EarI CTCTTC 7 cut(s) 1234, 1880, 3952, 4023, 4568, 4839, 5067
EciI GGCGGA 1 cut(s) 1761
Ecl136II GAGCTC 3 cut(s) 35, 3642, 4424
Eco105I TACGTA 1 cut(s) 1004
Eco130I CCWWGG 9 cut(s) 647, 834, 1490, 1701, 2283, 2708, 4244, 4495, 4565
Eco24I GRGCYC 5 cut(s) 14, 37, 1572, 3644, 4426
Eco32I GATATC 2 cut(s) 1027, 3707
Eco47I GGWCC 5 cut(s) 60, 220, 1871, 3143, 4032
Eco53kI GAGCTC 3 cut(s) 35, 3642, 4424
Eco57I CTGAAG 8 cut(s) 1901, 2481, 2577, 2601, 2964, 3992, 4107, 4458
Eco72I CACGTG 1 cut(s) 4640
Eco81I CCTNAGG 2 cut(s) 1145, 2406
Eco88I CYCGRG 1 cut(s) 2048
Eco91I GGTNACC 2 cut(s) 767, 4964
EcoICRI GAGCTC 3 cut(s) 35, 3642, 4424
EcoO109I RGGNCCY 1 cut(s) 2101
EcoO65I GGTNACC 2 cut(s) 767, 4964
EcoRI GAATTC 2 cut(s) 3928, 4442
EcoRII CCWGG 7 cut(s) 1335, 2303, 2533, 2698, 3264, 3322, 4098
EcoRV GATATC 2 cut(s) 1027, 3707
EcoT14I CCWWGG 9 cut(s) 647, 834, 1490, 1701, 2283, 2708, 4244, 4495, 4565
EcoT22I ATGCAT 3 cut(s) 424, 4864, 5084
EcoT38I GRGCYC 5 cut(s) 14, 37, 1572, 3644, 4426
ErhI CCWWGG 9 cut(s) 647, 834, 1490, 1701, 2283, 2708, 4244, 4495, 4565
Esp3I CGTCTC 4 cut(s) 615, 1027, 2364, 2532
FalI AAGNNNNNCTT 6 cut(s) 1681, 1713, 1796, 1828, 1970, 2002
FaqI GGGAC 3 cut(s) 1383, 2065, 2219
FauI CCCGC 2 cut(s) 757, 966
FauNDI CATATG 1 cut(s) 1774
FbaI TGATCA 2 cut(s) 2665, 4879
FriOI GRGCYC 5 cut(s) 14, 37, 1572, 3644, 4426
FspBI CTAG 8 cut(s) 1284, 1623, 1901, 2202, 3665, 4206, 4502, 4566
GlaI GCGC 3 cut(s) 327, 351, 2762
GsaI CCCAGC 2 cut(s) 359, 1634
GsuI CTGGAG 6 cut(s) 303, 2517, 3737, 4082, 4136, 4349
HaeII RGCGCY 1 cut(s) 2764
HapII CCGG 6 cut(s) 208, 236, 243, 1740, 2049, 5030
HgaI GACGC 3 cut(s) 241, 2639, 4735
HhaI GCGC 3 cut(s) 328, 352, 2763
Hin1I GRCGYC 2 cut(s) 233, 2631
Hin6I GCGC 3 cut(s) 326, 350, 2761
HinP1I GCGC 3 cut(s) 326, 350, 2761
HindIII AAGCTT 4 cut(s) 108, 1602, 3038, 4367
HpaII CCGG 6 cut(s) 208, 236, 243, 1740, 2049, 5030
Hpy166II GTNNAC 6 cut(s) 626, 1163, 1581, 2249, 4478, 4643
Hpy8I GTNNAC 6 cut(s) 626, 1163, 1581, 2249, 4478, 4643
Hpy99I CGWCG 3 cut(s) 136, 235, 5140
HpyCH4IV ACGT 7 cut(s) 1003, 2251, 2512, 2541, 2833, 4639, 4956
HpySE526I ACGT 7 cut(s) 1003, 2251, 2512, 2541, 2833, 4639, 4956
Hsp92I GRCGYC 2 cut(s) 233, 2631
HspAI GCGC 3 cut(s) 326, 350, 2761
Kpn2I TCCGGA 1 cut(s) 5029
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Ksp22I TGATCA 2 cut(s) 2665, 4879
LguI GCTCTTC 1 cut(s) 4023
MaeI CTAG 8 cut(s) 1284, 1623, 1901, 2202, 3665, 4206, 4502, 4566
MaeII ACGT 7 cut(s) 1003, 2251, 2512, 2541, 2833, 4639, 4956
MfeI CAATTG 2 cut(s) 1463, 3657
MhlI GDGCHC 8 cut(s) 14, 37, 1291, 1572, 1696, 3644, 4426, 4645
MlsI TGGCCA 1 cut(s) 478
MluNI TGGCCA 1 cut(s) 478
MlyI GAGTC 9 cut(s) 73, 146, 257, 398, 1205, 2038, 2201, 4342, 4510
MmeI TCCRAC 5 cut(s) 114, 375, 712, 915, 4397
Mox20I TGGCCA 1 cut(s) 478
Mph1103I ATGCAT 3 cut(s) 424, 4864, 5084
MreI CGCCGGCG 1 cut(s) 235
MroI TCCGGA 1 cut(s) 5029
MroNI GCCGGC 1 cut(s) 235
MroXI GAANNNNTTC 5 cut(s) 1596, 1985, 2562, 4017, 4092
MscI TGGCCA 1 cut(s) 478
MslI CAYNNNNRTG 7 cut(s) 618, 687, 951, 1614, 1706, 2921, 4887
Msp20I TGGCCA 1 cut(s) 478
MspA1I CMGCKG 5 cut(s) 355, 1822, 3796, 4085, 4268
MspI CCGG 6 cut(s) 208, 236, 243, 1740, 2049, 5030
MunI CAATTG 2 cut(s) 1463, 3657
Mva1269I GAATGC 5 cut(s) 1337, 1987, 2854, 3505, 5084
MvaI CCWGG 7 cut(s) 1337, 2305, 2535, 2700, 3266, 3324, 4100
MvnI CGCG 1 cut(s) 328
NaeI GCCGGC 1 cut(s) 237
NciI CCSGG 3 cut(s) 1740, 2049, 2050
NcoI CCATGG 5 cut(s) 834, 1701, 2708, 4244, 4495
NdeI CATATG 1 cut(s) 1774
NgoMIV GCCGGC 1 cut(s) 235
NlaIV GGNNCC 4 cut(s) 2403, 2611, 5028, 5044
NmeAIII GCCGAG 2 cut(s) 3240, 3920
NmuCI GTSAC 8 cut(s) 747, 767, 1910, 2551, 3189, 3218, 4201, 5150
NsiI ATGCAT 3 cut(s) 424, 4864, 5084
NspI RCATGY 5 cut(s) 3502, 3527, 3905, 4315, 4616
NspV TTCGAA 1 cut(s) 2815
OliI CACNNNNGTG 1 cut(s) 951
PagI TCATGA 5 cut(s) 910, 1609, 2662, 3709, 4389
PciI ACATGT 2 cut(s) 3498, 3901
PciSI GCTCTTC 1 cut(s) 4023
PcsI WCGNNNNNNNCGW 1 cut(s) 1593
PctI GAATGC 5 cut(s) 1337, 1987, 2854, 3505, 5084
PdiI GCCGGC 1 cut(s) 237
PdmI GAANNNNTTC 5 cut(s) 1596, 1985, 2562, 4017, 4092
PflFI GACNNNGTC 1 cut(s) 4976
PflMI CCANNNNNTGG 1 cut(s) 4105
PfoI TCCNGGA 2 cut(s) 1738, 2533
Ple19I CGATCG 1 cut(s) 181
PleI GAGTC 9 cut(s) 73, 145, 257, 398, 1204, 2038, 2201, 4341, 4510
PmaCI CACGTG 1 cut(s) 4640
PmlI CACGTG 1 cut(s) 4640
PpsI GAGTC 9 cut(s) 73, 145, 257, 398, 1204, 2038, 2201, 4341, 4510
Ppu21I YACGTR 3 cut(s) 1004, 2252, 4640
PscI ACATGT 2 cut(s) 3498, 3901
PshBI ATTAAT 1 cut(s) 864
PsiI TTATAA 2 cut(s) 4755, 4953
Psp124BI GAGCTC 3 cut(s) 37, 3644, 4426
Psp1406I AACGTT 1 cut(s) 2833
Psp6I CCWGG 7 cut(s) 1335, 2303, 2533, 2698, 3264, 3322, 4098
PspCI CACGTG 1 cut(s) 4640
PspEI GGTNACC 2 cut(s) 767, 4964
PspFI CCCAGC 2 cut(s) 355, 1630
PspGI CCWGG 7 cut(s) 1335, 2303, 2533, 2698, 3264, 3322, 4098
PspN4I GGNNCC 4 cut(s) 2403, 2611, 5028, 5044
PspPI GGNCC 7 cut(s) 60, 220, 380, 1871, 2101, 3143, 4032
PstI CTGCAG 2 cut(s) 790, 2302
PstNI CAGNNNCTG 3 cut(s) 3799, 4105, 4327
PsyI GACNNNGTC 1 cut(s) 4976
PvuI CGATCG 1 cut(s) 181
PvuII CAGCTG 4 cut(s) 355, 3796, 4085, 4268
RsaI GTAC 9 cut(s) 699, 853, 1441, 2250, 2292, 2425, 2738, 3589, 4617
RsaNI GTAC 9 cut(s) 698, 852, 1440, 2249, 2291, 2424, 2737, 3588, 4616
RseI CAYNNNNRTG 7 cut(s) 618, 687, 951, 1614, 1706, 2921, 4887
SacI GAGCTC 3 cut(s) 37, 3644, 4426
SapI GCTCTTC 1 cut(s) 4023
Sau96I GGNCC 7 cut(s) 60, 220, 380, 1871, 2101, 3143, 4032
ScaI AGTACT 3 cut(s) 853, 2292, 2738
SchI GAGTC 9 cut(s) 73, 146, 257, 398, 1205, 2038, 2201, 4342, 4510
SduI GDGCHC 8 cut(s) 14, 37, 1291, 1572, 1696, 3644, 4426, 4645
SfuI TTCGAA 1 cut(s) 2815
SgrAI CRCCGGYG 1 cut(s) 235
SinI GGWCC 5 cut(s) 60, 220, 1871, 3143, 4032
SmaI CCCGGG 1 cut(s) 2050
SmiMI CAYNNNNRTG 7 cut(s) 618, 687, 951, 1614, 1706, 2921, 4887
SmlI CTYRAG 3 cut(s) 101, 2087, 2825
SmoI CTYRAG 3 cut(s) 101, 2087, 2825
SnaBI TACGTA 1 cut(s) 1004
SspI AATATT 5 cut(s) 1110, 1839, 3471, 3756, 4139
SspMI CTAG 8 cut(s) 1284, 1623, 1901, 2202, 3665, 4206, 4502, 4566
SstI GAGCTC 3 cut(s) 37, 3644, 4426
StyI CCWWGG 9 cut(s) 647, 834, 1490, 1701, 2283, 2708, 4244, 4495, 4565
TaiI ACGT 7 cut(s) 1006, 2254, 2515, 2544, 2836, 4642, 4959
TaqI TCGA 9 cut(s) 314, 1587, 2183, 2210, 2815, 3247, 3313, 3361, 4184
TaqII GACCGA 2 cut(s) 2969, 4049
TatI WGTACW 5 cut(s) 851, 1439, 2290, 2736, 4615
TauI GCSGC 5 cut(s) 253, 415, 1846, 4301, 4808
TseFI GTSAC 8 cut(s) 747, 767, 1910, 2551, 3189, 3218, 4201, 5150
Tsp45I GTSAC 8 cut(s) 747, 767, 1910, 2551, 3189, 3218, 4201, 5150
TspGWI ACGGA 3 cut(s) 2291, 3097, 3605
TspMI CCCGGG 1 cut(s) 2048
Tth111I GACNNNGTC 1 cut(s) 4976
Van91I CCANNNNNTGG 1 cut(s) 4105
VneI GTGCAC 1 cut(s) 4641
VpaK11BI GGWCC 5 cut(s) 60, 220, 1871, 3143, 4032
VspI ATTAAT 1 cut(s) 864
XapI RAATTY 6 cut(s) 2320, 3825, 3928, 4150, 4442, 4874
XceI RCATGY 5 cut(s) 3502, 3527, 3905, 4315, 4616
XmaI CCCGGG 1 cut(s) 2048
XmaJI CCTAGG 1 cut(s) 4565
XmnI GAANNNNTTC 5 cut(s) 1596, 1985, 2562, 4017, 4092
XspI CTAG 8 cut(s) 1284, 1623, 1901, 2202, 3665, 4206, 4502, 4566
ZrmI AGTACT 3 cut(s) 853, 2292, 2738
Zsp2I ATGCAT 3 cut(s) 424, 4864, 5084
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.