Rh2CG315100

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0002907
Physical Location & Seq
Reverse (-)
39857232 .. 39871863
14632 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG315100.1

Sequence Viewer

Length: 4233 bp
ATGGAGACTGGCTCGGAGGGAGAGAAGACGAGAGCTGAGGCTCAGGCTCAGGAAGGGGAGATCAGAACCAAGCGCAAAATGAAGACTCGTTCGCAGTTGGAACTTCTTGAGCAAGCTTATGCAGCTGAGCAATACCCGTCGGAGTCGGTGAGAGCTGAGCTGTCGGCGAAATTGGATCTTTCCGATCGGCAACTACAGATGTGGTTCTGCCACCGGAGATTGAAGGACCGTAAGCCGACGCCGACGACCGGGAAGCGGCAGAGGAAGGACTCTCCGGCTGGAACGACTGGAGTGGTTTCTGAGGAAATGGCGGGGAGTGAGCGGCCGCCAAAGCAGTTGATAATGGAGGCTCGAGCTGCTGCTTTTGTGGAGGCGCAGCTGGGAGAGCCATTCAGGGAGGACGGGCCGATACTGGGCTTGGAGTTTGATCCGTTGCCACCGGGCGCATTTGGTTCCTCCGTAGGAGTGGCAACTGTTGGGCAGCAGAAGCAATCGGGATGGCCATATTCTGCAAATATATATGAGCAGTCTAATGCTAAACCAATTAAGGGTGCTTCAAGGACTGTTCATGAATACCAGTTTCTTCCAGAAAAGCCAACTGTTAGGACAGACACTTATGAAAGGGCAGCCCCATCTCACCACTATGGTTCACCCACTGAATCTCGCCATGCAAGGATTCCATTATCTACTGTGTCCTCGTTCATTCATGGTAATGAACAAGTGCCCACTGTATATCGATTTCAAAGCCAGATGGCTGGGCTAAATCTTTTGTCACAGCAAGGCAGGGCTGGTCACCTCTTGCCATCTGCTTCAGAAGAATATGGCAATGTTCATGGAAAGAACTTCACGAATATTACCATGGATGCTCATTTTAGTGCTCACCCAATTAATCAACTGAACAATCCTTTTATACCATTTGCCAGAAGTGTGGCTCATGACGAGGATGATGTTTTAAGATTGGAAAGGAAGCACAAGAGTGAAGAAACAAGAATAATGCGGGAAGTTGAAGCCCATGAGAAAAGGATACGTAAAGAGCTTGAAAAACAAGATATCCTGAGACGAAAGAGAGAAGAGCAGATAAGGAAAGAAATGGAGAGGATTGATCGGGAGAGGAGGAAGGAAGAAGAAAGGCTATTGCGTGAAAAGCAGAGAGAGGAGGAGAGATACCTAAGGGAGCAAAGGCGTGAACTGGAGCGAAAGGAGAAATTCTTACAGAAGGAGTCAATAAGAGCTGAGAAAATGAGACAAAAAGAAGAATTACGCAGAGAGAAAGAGGCAGCAAGGCTTAAAGCTGCTAATGAGAGAGCTATTGCTCGTAAAAATGCTAAAGAATCAATGGAACTCATTGAGGATGAATGCCTGGAACTCATGGAGTTAGCTGCATCAAGCAAGGAGCTTCCTTCCATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGATATGCGGAGTACATTCCCACCCAAATCTGTGCAGTTGAAAAAACCTTTTGGAATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGGCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAATACCCTCTATGGGGGTGGGAGCTAATCAGAACTGCGCTGCAAATCCTGGAGGCGGTCACCCTCAAATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACTTAAATCCACTGACATGGCCAGAAATATTGCGGCAATTTTCTGTATCAGCAGGATTTGGACCGCAACTGAAGAAGAGGGATGTTGAACTAGCTTATCTTCATGAGGACAATGAGGGTAATGATGTCAAGGATATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTCGCTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGCAAATCAAGGCATTGCTTGACTCCTGGCACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGAACTGCACCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACAAGTGATTCCGAGGCAGTACTTTCGGCAGCCAGAGAAAGAATTCAAATATTCAATAGTAGGATTTTTGATGTTGAAGGAGCTGATGAAGCTGAAAGAGACGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGTTTGATGATTTAGGCACTGAAATAAATCCAAAGATAGTAGCTCAGAATTCTGAAGAAGCAAGAAAAGTCAGTGACGGAATATCTTTGGAAAGTGGAAAAGGAAGCTATGAGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAGAAGGAATTCCTTCAATCAATTCTGAAGCGTCCATCAAGGTTGAAGACGCTGGTTGTTCCTTGGATTCTGTTGACCCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAACCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCATTAATTGGTGTGGCAATAGAAGGAAACTCGATTCGAATTGTTCTCGAGGAACGTTTAGATGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGCACAAGTTGATAAACGGCGTCTAAAAGAAGAACATGTCATCAAAATGCAATATACCCCTTTCATTTGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCAGAAAATAGGCAAAGTCCATCTCCTACCGTTGATGAGAAAAACATTGAGACGACGGTGAAGCTTTCCGTCCTGCAAGAGCAGATGGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCTTCTGTAGGAAACCTCCAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTATTGCTGATAAGTCGCACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCATTGCCTCTTGGTCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGCAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGTGCGTGGGTATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTTAAGGAAACTGTTAGAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATCAAAGACACTGCTAAAACAGAAGATGTTGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACGGATAGCCCTACCAGCTCTGTTTCTGTTGCTGACTCCGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAACGAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGATCTGGAGAGGTGGATTTTGAAAGAAAGTGTTGGTTCTTCAATATTATGTGCTGACAAAAATGGGGAAAAAAGGTGTCCCCAGCTACTGGACATCTGCAATTCTTGTCGTGGAATTTTTTATTTTGAAGAGAATCACTGTCATTCTTGCCATAGAACTTTTGGTAAGGGCGAAATAGATTTTTCACAACATGTGGTACTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGAGTTCTTCTCCCCTTAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAAATCTTGGGGTAGAAAGTTACATTCCTCGTCAACAGCTGAAGACCTTCTCCAGGTTACAAATTGGCATTGCTCCCTATTAATGAAAAACTTTTTCTTTTTCTTTTGGAACATGTGTAAAAAAGATGTAGAGAGGCACTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1410

Amino Acids

160.15

Weight (kDa)

5.86

Isoelectric Point (pI)

53.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 22 - 78 6.3e-16 Homeodomain
DDT PF02791 511 - 566 1e-18 DDT domain
HARE-HTH PF05066 692 - 759 9.4e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 908 - 951 3.1e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1090 - 1163 1.3e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 3823
AccBSI CCGCTC 1 cut(s) 322
AciI CCGC 9 cut(s) 256, 311, 322, 326, 997, 1459, 1770, 1867, 1898
AclI AACGTT 1 cut(s) 2857
AcoI YGGCCR 4 cut(s) 323, 500, 1598, 1853
AcsI RAATTY 8 cut(s) 1205, 1506, 1616, 2337, 2479, 2586, 3849, 3958
AcuI CTGAAG 9 cut(s) 795, 1925, 2505, 2625, 2985, 3426, 3971, 4034, 4149
AcyI GRCGYC 2 cut(s) 239, 2920
AfaI GTAC 7 cut(s) 1465, 2274, 2316, 2762, 3158, 3613, 3933
AflIII ACRYGT 3 cut(s) 2935, 3925, 4200
AjnI CCWGG 7 cut(s) 1359, 1762, 2071, 2557, 2722, 3346, 4140
AjuI GAANNNNNNNTTGG 2 cut(s) 1498, 1530
AleI CACNNNNGTG 1 cut(s) 975
Alw21I GWGCWC 3 cut(s) 880, 2281, 3668
Alw26I GTCTC 6 cut(s) 1051, 1237, 1416, 2388, 2556, 3044
AlwNI CAGNNNCTG 2 cut(s) 2989, 3823
Ama87I CYCGRG 3 cut(s) 351, 2849, 3982
AoxI GGCC 6 cut(s) 323, 404, 500, 1598, 1853, 2125
ApoI RAATTY 8 cut(s) 1205, 1506, 1616, 2337, 2479, 2586, 3849, 3958
AseI ATTAAT 3 cut(s) 888, 2807, 4169
Asp700I GAANNNNTTC 6 cut(s) 1620, 2337, 2586, 2590, 4059, 4134
AspLEI GCGC 5 cut(s) 75, 376, 446, 1754, 2787
AspS9I GGNCC 6 cut(s) 226, 404, 1895, 2125, 3167, 4074
AsuC2I CCSGG 4 cut(s) 250, 441, 3195, 3539
AsuII TTCGAA 1 cut(s) 2839
AvaI CYCGRG 3 cut(s) 351, 2849, 3982
AvaII GGWCC 4 cut(s) 226, 1895, 3167, 4074
AxyI CCTNAGG 3 cut(s) 1169, 2567, 3998
BaeGI GKGCMC 1 cut(s) 726
BalI TGGCCA 3 cut(s) 502, 1600, 1855
BamHI GGATCC 1 cut(s) 2425
BanII GRGCYC 1 cut(s) 3668
BbsI GAAGAC 5 cut(s) 32, 89, 2631, 3491, 4137
Bbv12I GWGCWC 3 cut(s) 880, 2281, 3668
BbvCI CCTCAGC 1 cut(s) 36
BccI CCATC 9 cut(s) 492, 640, 745, 811, 1694, 2621, 3028, 3079, 3413
BceAI ACGGC 1 cut(s) 2933
BcgI CGANNNNNNTGC 4 cut(s) 2301, 2335, 3365, 3399
BciT130I CCWGG 7 cut(s) 1361, 1764, 2073, 2559, 2724, 3348, 4142
BciVI GTATCC 1 cut(s) 1017
BclI TGATCA 1 cut(s) 2689
BcnI CCSGG 4 cut(s) 250, 441, 3195, 3539
BcoDI GTCTC 6 cut(s) 1051, 1237, 1416, 2388, 2556, 3044
BfaI CTAG 4 cut(s) 1647, 1925, 2226, 3689
BfmI CTRYAG 4 cut(s) 194, 2284, 2658, 3132
BfoI RGCGCY 1 cut(s) 2788
BfuI GTATCC 1 cut(s) 1017
BglII AGATCT 1 cut(s) 3736
BlpI GCTNAGC 2 cut(s) 126, 156
BmcAI AGTACT 3 cut(s) 2316, 2762, 3158
Bme18I GGWCC 4 cut(s) 226, 1895, 3167, 4074
BmeT110I CYCGRG 3 cut(s) 351, 2849, 3982
BmgT120I GGNCC 6 cut(s) 226, 404, 1895, 2125, 3167, 4074
BmiI GGNNCC 2 cut(s) 454, 2427
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 5 cut(s) 853, 1391, 2404, 2854, 3440
BmuI ACTGGG 1 cut(s) 422
BpiI GAAGAC 5 cut(s) 32, 89, 2631, 3491, 4137
BplI GAGNNNNNCTC 3 cut(s) 28, 3976, 4008
BpmI CTGGAG 6 cut(s) 309, 1211, 1785, 2541, 3761, 4124
Bpu10I CCTNAGC 3 cut(s) 36, 42, 48
Bpu1102I GCTNAGC 2 cut(s) 126, 156
Bpu14I TTCGAA 1 cut(s) 2839
BpuEI CTTGAG 2 cut(s) 128, 2132
BpuMI CCSGG 4 cut(s) 250, 441, 3195, 3539
Bsa29I ATCGAT 2 cut(s) 736, 3271
BsaAI YACGTR 3 cut(s) 1028, 2276, 3977
BsaHI GRCGYC 2 cut(s) 239, 2920
BsaI GGTCTC 1 cut(s) 2556
BsaJI CCNNGG 7 cut(s) 858, 1514, 2307, 2640, 2722, 2732, 3538
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 2 cut(s) 1983, 2013
Bse1I ACTGG 8 cut(s) 13, 292, 417, 577, 1194, 3317, 3718, 3828
Bse21I CCTNAGG 3 cut(s) 1169, 2567, 3998
Bse3DI GCAATG 4 cut(s) 832, 2059, 3275, 4156
BseBI CCWGG 7 cut(s) 1361, 1764, 2073, 2559, 2724, 3348, 4142
BseCI ATCGAT 2 cut(s) 736, 3271
BseDI CCNNGG 7 cut(s) 858, 1514, 2307, 2640, 2722, 2732, 3538
BseGI GGATG 7 cut(s) 503, 868, 949, 1357, 1588, 1921, 3445
BseMI GCAATG 4 cut(s) 832, 2059, 3275, 4156
BseNI ACTGG 8 cut(s) 13, 292, 417, 577, 1194, 3317, 3718, 3828
BseRI GAGGAG 6 cut(s) 1126, 1169, 1172, 1544, 2795, 3269
BseSI GKGCMC 1 cut(s) 726
BseX3I CGGCCG 1 cut(s) 323
BseYI CCCAGC 3 cut(s) 379, 755, 3816
BsgI GTGCAG 2 cut(s) 1505, 2238
Bsh1285I CGRYCG 4 cut(s) 187, 249, 326, 3385
BshFI GGCC 6 cut(s) 325, 406, 502, 1600, 1855, 2127
BshVI ATCGAT 2 cut(s) 736, 3271
BsiEI CGRYCG 4 cut(s) 187, 249, 326, 3385
BsiHKAI GWGCWC 3 cut(s) 880, 2281, 3668
BsiHKCI CYCGRG 3 cut(s) 351, 2849, 3982
BsiSI CCGG 6 cut(s) 214, 249, 275, 440, 3195, 3538
BslFI GGGAC 2 cut(s) 2243, 3798
BsmAI GTCTC 6 cut(s) 1051, 1237, 1416, 2388, 2556, 3044
BsmBI CGTCTC 3 cut(s) 1051, 2388, 3044
BsmFI GGGAC 2 cut(s) 2243, 3798
BsmI GAATGC 2 cut(s) 1361, 3529
BsnI GGCC 6 cut(s) 325, 406, 502, 1600, 1855, 2127
Bso31I GGTCTC 1 cut(s) 2556
BsoBI CYCGRG 3 cut(s) 351, 2849, 3982
Bsp119I TTCGAA 1 cut(s) 2839
Bsp1286I GDGCHC 4 cut(s) 726, 880, 2281, 3668
Bsp1720I GCTNAGC 2 cut(s) 126, 156
Bsp19I CCATGG 2 cut(s) 858, 2732
BspACI CCGC 9 cut(s) 256, 311, 322, 326, 997, 1459, 1770, 1867, 1898
BspANI GGCC 6 cut(s) 325, 406, 502, 1600, 1855, 2127
BspDI ATCGAT 2 cut(s) 736, 3271
BspHI TCATGA 5 cut(s) 568, 934, 1633, 1936, 2686
BspLI GGNNCC 2 cut(s) 454, 2427
BspMAI CTGCAG 1 cut(s) 2662
BspQI GCTCTTC 2 cut(s) 1065, 4065
BspT104I TTCGAA 1 cut(s) 2839
BspTNI GGTCTC 1 cut(s) 2556
BsrBI CCGCTC 1 cut(s) 322
BsrDI GCAATG 4 cut(s) 832, 2059, 3275, 4156
BsrI ACTGG 8 cut(s) 13, 292, 417, 577, 1194, 3317, 3718, 3828
BssECI CCNNGG 7 cut(s) 858, 1514, 2307, 2640, 2722, 2732, 3538
BssNI GRCGYC 2 cut(s) 239, 2920
BssT1I CCWWGG 4 cut(s) 858, 1514, 2640, 2732
Bst2UI CCWGG 7 cut(s) 1361, 1764, 2073, 2559, 2724, 3348, 4142
Bst6I CTCTTC 5 cut(s) 1065, 1904, 3402, 3858, 4065
BstACI GRCGYC 2 cut(s) 239, 2920
BstAPI GCANNNNNTGC 1 cut(s) 2801
BstBAI YACGTR 3 cut(s) 1028, 2276, 3977
BstBI TTCGAA 1 cut(s) 2839
BstC8I GCNNGC 1 cut(s) 114
BstDSI CCRYGG 2 cut(s) 858, 2732
BstEII GGTNACC 2 cut(s) 791, 1772
BstENI CCTNNNNNAGG 2 cut(s) 3132, 4139
BstF5I GGATG 7 cut(s) 503, 868, 949, 1357, 1588, 1921, 3445
BstH2I RGCGCY 1 cut(s) 2788
BstHHI GCGC 5 cut(s) 75, 376, 446, 1754, 2787
BstMAI GTCTC 6 cut(s) 1051, 1237, 1416, 2388, 2556, 3044
BstMCI CGRYCG 4 cut(s) 187, 249, 326, 3385
BstNI CCWGG 7 cut(s) 1361, 1764, 2073, 2559, 2724, 3348, 4142
BstNSI RCATGY 3 cut(s) 2939, 3929, 4204
BstPI GGTNACC 2 cut(s) 791, 1772
BstSFI CTRYAG 4 cut(s) 194, 2284, 2658, 3132
BstSLI GKGCMC 1 cut(s) 726
BstSNI TACGTA 1 cut(s) 1028
BstV2I GAAGAC 5 cut(s) 32, 89, 2631, 3491, 4137
BstX2I RGATCY 6 cut(s) 175, 1682, 2176, 2425, 3360, 3736
BstXI CCANNNNNNTGG 3 cut(s) 755, 928, 1851
BstYI RGATCY 6 cut(s) 175, 1682, 2176, 2425, 3360, 3736
BstZI CGGCCG 1 cut(s) 323
Bsu15I ATCGAT 2 cut(s) 736, 3271
Bsu36I CCTNAGG 3 cut(s) 1169, 2567, 3998
BsuI GTATCC 1 cut(s) 1017
BsuRI GGCC 6 cut(s) 325, 406, 502, 1600, 1855, 2127
BsuTUI ATCGAT 2 cut(s) 736, 3271
BtgI CCRYGG 2 cut(s) 858, 2732
BtsCI GGATG 7 cut(s) 503, 868, 949, 1357, 1588, 1921, 3445
BtsI GCAGTG 4 cut(s) 1993, 2904, 3558, 3610
Cac8I GCNNGC 1 cut(s) 114
CaiI CAGNNNCTG 2 cut(s) 2989, 3823
CciI TCATGA 5 cut(s) 568, 934, 1633, 1936, 2686
CciNI GCGGCCGC 1 cut(s) 323
CfoI GCGC 5 cut(s) 75, 376, 446, 1754, 2787
Cfr13I GGNCC 6 cut(s) 226, 404, 1895, 2125, 3167, 4074
ClaI ATCGAT 2 cut(s) 736, 3271
CseI GACGC 4 cut(s) 247, 2598, 2636, 2909
Csp6I GTAC 7 cut(s) 1464, 2273, 2315, 2761, 3157, 3612, 3932
CviQI GTAC 7 cut(s) 1464, 2273, 2315, 2761, 3157, 3612, 3932
DraI TTTAAA 2 cut(s) 2880, 3723
EaeI YGGCCR 4 cut(s) 323, 500, 1598, 1853
EagI CGGCCG 1 cut(s) 323
Eam1104I CTCTTC 5 cut(s) 1065, 1904, 3402, 3858, 4065
EarI CTCTTC 5 cut(s) 1065, 1904, 3402, 3858, 4065
Ecl136II GAGCTC 1 cut(s) 3666
EclXI CGGCCG 1 cut(s) 323
Eco105I TACGTA 1 cut(s) 1028
Eco130I CCWWGG 4 cut(s) 858, 1514, 2640, 2732
Eco24I GRGCYC 1 cut(s) 3668
Eco31I GGTCTC 1 cut(s) 2556
Eco32I GATATC 2 cut(s) 1051, 3731
Eco47I GGWCC 4 cut(s) 226, 1895, 3167, 4074
Eco52I CGGCCG 1 cut(s) 323
Eco53kI GAGCTC 1 cut(s) 3666
Eco57I CTGAAG 9 cut(s) 795, 1925, 2505, 2625, 2985, 3426, 3971, 4034, 4149
Eco81I CCTNAGG 3 cut(s) 1169, 2567, 3998
Eco88I CYCGRG 3 cut(s) 351, 2849, 3982
Eco91I GGTNACC 2 cut(s) 791, 1772
EcoICRI GAGCTC 1 cut(s) 3666
EcoNI CCTNNNNNAGG 2 cut(s) 3132, 4139
EcoO109I RGGNCCY 1 cut(s) 2125
EcoO65I GGTNACC 2 cut(s) 791, 1772
EcoRI GAATTC 6 cut(s) 1506, 1616, 2337, 2479, 2586, 3958
EcoRII CCWGG 7 cut(s) 1359, 1762, 2071, 2557, 2722, 3346, 4140
EcoRV GATATC 2 cut(s) 1051, 3731
EcoT14I CCWWGG 4 cut(s) 858, 1514, 2640, 2732
EcoT38I GRGCYC 1 cut(s) 3668
ErhI CCWWGG 4 cut(s) 858, 1514, 2640, 2732
Esp3I CGTCTC 3 cut(s) 1051, 2388, 3044
FalI AAGNNNNNCTT 2 cut(s) 1820, 1852
FaqI GGGAC 2 cut(s) 2243, 3798
FauI CCCGC 2 cut(s) 304, 990
FauNDI CATATG 1 cut(s) 1798
FbaI TGATCA 1 cut(s) 2689
FokI GGATG 7 cut(s) 510, 875, 956, 1364, 1595, 1928, 3452
FriOI GRGCYC 1 cut(s) 3668
FspBI CTAG 4 cut(s) 1647, 1925, 2226, 3689
GlaI GCGC 5 cut(s) 74, 375, 445, 1753, 2786
GsaI CCCAGC 3 cut(s) 383, 759, 3820
GsuI CTGGAG 6 cut(s) 309, 1211, 1785, 2541, 3761, 4124
HaeII RGCGCY 1 cut(s) 2788
HaeIII GGCC 6 cut(s) 325, 406, 502, 1600, 1855, 2127
HapII CCGG 6 cut(s) 214, 249, 275, 440, 3195, 3538
HgaI GACGC 4 cut(s) 247, 2598, 2636, 2909
HhaI GCGC 5 cut(s) 75, 376, 446, 1754, 2787
Hin1I GRCGYC 2 cut(s) 239, 2920
Hin6I GCGC 5 cut(s) 73, 374, 444, 1752, 2785
HinP1I GCGC 5 cut(s) 73, 374, 444, 1752, 2785
HincII GTYRAC 2 cut(s) 2653, 4122
HindII GTYRAC 2 cut(s) 2653, 4122
HindIII AAGCTT 3 cut(s) 114, 1626, 3062
HpaII CCGG 6 cut(s) 214, 249, 275, 440, 3195, 3538
Hpy166II GTNNAC 5 cut(s) 650, 1187, 2273, 2653, 4122
Hpy8I GTNNAC 5 cut(s) 650, 1187, 2273, 2653, 4122
Hpy99I CGWCG 4 cut(s) 142, 241, 247, 3058
HpyCH4IV ACGT 4 cut(s) 1027, 2275, 2857, 3976
HpySE526I ACGT 4 cut(s) 1027, 2275, 2857, 3976
Hsp92I GRCGYC 2 cut(s) 239, 2920
HspAI GCGC 5 cut(s) 73, 374, 444, 1752, 2785
Ksp22I TGATCA 1 cut(s) 2689
LguI GCTCTTC 2 cut(s) 1065, 4065
LweI GCATC 5 cut(s) 853, 1391, 2404, 2854, 3440
MaeI CTAG 4 cut(s) 1647, 1925, 2226, 3689
MaeII ACGT 4 cut(s) 1027, 2275, 2857, 3976
MaeIII GTNAC 8 cut(s) 771, 791, 1772, 2504, 2575, 3242, 4107, 4144
MbiI CCGCTC 1 cut(s) 322
MfeI CAATTG 1 cut(s) 3681
MflI RGATCY 6 cut(s) 175, 1682, 2176, 2425, 3360, 3736
MhlI GDGCHC 4 cut(s) 726, 880, 2281, 3668
MlsI TGGCCA 3 cut(s) 502, 1600, 1855
MluNI TGGCCA 3 cut(s) 502, 1600, 1855
MlyI GAGTC 7 cut(s) 79, 152, 263, 1229, 2062, 2225, 3641
MmeI TCCRAC 4 cut(s) 78, 120, 3368, 3639
Mox20I TGGCCA 3 cut(s) 502, 1600, 1855
MroXI GAANNNNTTC 6 cut(s) 1620, 2337, 2586, 2590, 4059, 4134
MscI TGGCCA 3 cut(s) 502, 1600, 1855
MslI CAYNNNNRTG 7 cut(s) 642, 711, 873, 975, 1638, 1849, 2945
Msp20I TGGCCA 3 cut(s) 502, 1600, 1855
MspA1I CMGCKG 3 cut(s) 125, 379, 4127
MspI CCGG 6 cut(s) 214, 249, 275, 440, 3195, 3538
MunI CAATTG 1 cut(s) 3681
Mva1269I GAATGC 2 cut(s) 1361, 3529
MvaI CCWGG 7 cut(s) 1361, 1764, 2073, 2559, 2724, 3348, 4142
NciI CCSGG 4 cut(s) 250, 441, 3195, 3539
NcoI CCATGG 2 cut(s) 858, 2732
NdeI CATATG 1 cut(s) 1798
NlaIV GGNNCC 2 cut(s) 454, 2427
NmuCI GTSAC 6 cut(s) 771, 791, 1772, 2504, 2575, 3242
NotI GCGGCCGC 1 cut(s) 323
NspI RCATGY 3 cut(s) 2939, 3929, 4204
NspV TTCGAA 1 cut(s) 2839
OliI CACNNNNGTG 1 cut(s) 975
PaeR7I CTCGAG 3 cut(s) 351, 2849, 3982
PagI TCATGA 5 cut(s) 568, 934, 1633, 1936, 2686
PciI ACATGT 3 cut(s) 2935, 3925, 4200
PciSI GCTCTTC 2 cut(s) 1065, 4065
PcsI WCGNNNNNNNCGW 1 cut(s) 1617
PctI GAATGC 2 cut(s) 1361, 3529
PdmI GAANNNNTTC 6 cut(s) 1620, 2337, 2586, 2590, 4059, 4134
PflMI CCANNNNNTGG 1 cut(s) 3823
PfoI TCCNGGA 2 cut(s) 1762, 2557
Ple19I CGATCG 1 cut(s) 187
PleI GAGTC 7 cut(s) 79, 151, 263, 1228, 2062, 2225, 3641
PpsI GAGTC 7 cut(s) 79, 151, 263, 1228, 2062, 2225, 3641
Ppu21I YACGTR 3 cut(s) 1028, 2276, 3977
PscI ACATGT 3 cut(s) 2935, 3925, 4200
PshBI ATTAAT 3 cut(s) 888, 2807, 4169
Psp124BI GAGCTC 1 cut(s) 3668
Psp1406I AACGTT 1 cut(s) 2857
Psp6I CCWGG 7 cut(s) 1359, 1762, 2071, 2557, 2722, 3346, 4140
PspEI GGTNACC 2 cut(s) 791, 1772
PspFI CCCAGC 3 cut(s) 379, 755, 3816
PspGI CCWGG 7 cut(s) 1359, 1762, 2071, 2557, 2722, 3346, 4140
PspN4I GGNNCC 2 cut(s) 454, 2427
PspPI GGNCC 6 cut(s) 226, 404, 1895, 2125, 3167, 4074
PspXI VCTCGAGB 2 cut(s) 351, 3982
PstI CTGCAG 1 cut(s) 2662
PstNI CAGNNNCTG 2 cut(s) 2989, 3823
PsuI RGATCY 6 cut(s) 175, 1682, 2176, 2425, 3360, 3736
PvuI CGATCG 1 cut(s) 187
PvuII CAGCTG 3 cut(s) 125, 379, 4127
RsaI GTAC 7 cut(s) 1465, 2274, 2316, 2762, 3158, 3613, 3933
RsaNI GTAC 7 cut(s) 1464, 2273, 2315, 2761, 3157, 3612, 3932
RseI CAYNNNNRTG 7 cut(s) 642, 711, 873, 975, 1638, 1849, 2945
SacI GAGCTC 1 cut(s) 3668
SapI GCTCTTC 2 cut(s) 1065, 4065
Sau96I GGNCC 6 cut(s) 226, 404, 1895, 2125, 3167, 4074
SbfI CCTGCAGG 1 cut(s) 2662
ScaI AGTACT 3 cut(s) 2316, 2762, 3158
SchI GAGTC 7 cut(s) 79, 152, 263, 1229, 2062, 2225, 3641
SdaI CCTGCAGG 1 cut(s) 2662
SduI GDGCHC 4 cut(s) 726, 880, 2281, 3668
SfaNI GCATC 5 cut(s) 853, 1391, 2404, 2854, 3440
SfcI CTRYAG 4 cut(s) 194, 2284, 2658, 3132
Sfr274I CTCGAG 3 cut(s) 351, 2849, 3982
SfuI TTCGAA 1 cut(s) 2839
SinI GGWCC 4 cut(s) 226, 1895, 3167, 4074
SlaI CTCGAG 3 cut(s) 351, 2849, 3982
SmiMI CAYNNNNRTG 7 cut(s) 642, 711, 873, 975, 1638, 1849, 2945
SmlI CTYRAG 5 cut(s) 107, 351, 2111, 2849, 3982
SmoI CTYRAG 5 cut(s) 107, 351, 2111, 2849, 3982
SnaBI TACGTA 1 cut(s) 1028
Sse8387I CCTGCAGG 1 cut(s) 2662
SsiI CCGC 9 cut(s) 256, 311, 322, 326, 997, 1459, 1770, 1867, 1898
SspI AATATT 5 cut(s) 853, 1863, 2346, 3495, 3780
SspMI CTAG 4 cut(s) 1647, 1925, 2226, 3689
SstI GAGCTC 1 cut(s) 3668
StyI CCWWGG 4 cut(s) 858, 1514, 2640, 2732
TaiI ACGT 4 cut(s) 1030, 2278, 2860, 3979
TaqII GACCGA 1 cut(s) 4091
TatI WGTACW 4 cut(s) 1463, 2314, 2760, 3156
TauI GCSGC 4 cut(s) 259, 325, 328, 1870
TseFI GTSAC 6 cut(s) 771, 791, 1772, 2504, 2575, 3242
Tsp45I GTSAC 6 cut(s) 771, 791, 1772, 2504, 2575, 3242
TspGWI ACGGA 5 cut(s) 420, 448, 2523, 3058, 3629
Van91I CCANNNNNTGG 1 cut(s) 3823
VpaK11BI GGWCC 4 cut(s) 226, 1895, 3167, 4074
VspI ATTAAT 3 cut(s) 888, 2807, 4169
XagI CCTNNNNNAGG 2 cut(s) 3132, 4139
XapI RAATTY 8 cut(s) 1205, 1506, 1616, 2337, 2479, 2586, 3849, 3958
XceI RCATGY 3 cut(s) 2939, 3929, 4204
XcmI CCANNNNNNNNNTGG 1 cut(s) 3893
XhoI CTCGAG 3 cut(s) 351, 2849, 3982
XmnI GAANNNNTTC 6 cut(s) 1620, 2337, 2586, 2590, 4059, 4134
XspI CTAG 4 cut(s) 1647, 1925, 2226, 3689
ZrmI AGTACT 3 cut(s) 2316, 2762, 3158
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.