Rroxscaffold_2G00115760

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002907
Physical Location & Seq
Forward (+)
42227467 .. 42241691
14225 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00115760.1

Sequence Viewer

Length: 4959 bp
ATGTGGTTTTGCCACCGGAGATTGAAGGACCGTAAGCCGACGCCGACCACCGGAAAGCGGCAGAGGAATGACTCTCCGGCTGGAACGACTGGAGTGGTTTCTGAGGTAATGGCGGGGAGTGAGCCGCCGCCGCAGCAGTTGATAATAGATGCTCGAGCTGTTGATTTTGTGGAGGCGCAGCTGGGGGAGCCATTCAGGGAGGATGGGCCAATACTGGGCTTGGAGTTTGATCCGTTGCCACCGGGTGCATTTGGTTCCTCCGTAGGAGTGGCAACTGTTGGGCAGCAGAAGCAATCTGGATGGCCATATGATGCAAATATATATGAGCAGTCTGATGCTAAACCAATTAAGGGTGCTTCAAGGACTGTCCATGAATACCAGTTTCTTCCAGAAAAGCCAACTGTTAGGACAGACACTTATGAAAGGGCTGCCCCATCTCACCGCTATGGTTCACCCACTGAATCTCGCCATGCAAGGTTTCCATTATCTACTGTGTCCTCGTTCATTCATGGTAATGAACAAGTACCCACTGTATATGGATTTCAAAGCCAGATGGCTGGACTAAATCTTTTGCCACAGCAAGGCAGGGCTGCTCACCTCTTGCCATCTGCTTCAGAAGAATATGGCAATGTTCATGGAAAGAACTTCACGAATATTACCATGGATGCTTATTTTAGTGCTCACCCGATTAATCAACTGAACAATCCTTTTATACCATTTGCCAGAAGGGTGGCTCATGACGAGGATGATGTTCTAAGATTGGAAAGGAAGAACAAGAGTGAAGAAGCAAGAATAATGCGGGAAGTTGAAGCCCATGAGAAAAGGATACGTAAAGAGCTTGAAAAACAAGATATCCTGAGACGAAAGAGAGAGGAGCAGATAAGGAAAGAAATGGAGAGGATTGATCGGGAGAGGAGGAAGGAAGAGGAAAGGCTATTGCGTGAAAAGCAGAGAGAGGAGGAGAGATACCTAAGGGAGCAAAAGCGTGAACTGGAACGAAAGGAGAAATTCTTACAGAAGGAGTCAATAAGAGCTGAGAAAATGAGACAAAAAGAAGAATTACGCAGAGAGAAAGAGGCAGCAAGGCTCAAAGCTGCTAATGAGAGAGCTATTGCTCGTAAAAATGCTAAAGAATCAATGGAACTCATTGAGGATGAATGCTTGGAACTCATGGAGTTAGCTGCATCAAGCAAGGGACTTCCTTCCATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGATATGCAGAGTACATTCCCACCCAAATCTGTGCAGTTGAAAAAACCTTTTGGTATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGGCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAACACCCTCTATGGGGTGGGAGCTAATCAGAACTGCACTGCAAATCCTGGGGCGGTCACCCTCAGATTGTAGAAGGGGTAAAAACACTGCGGTTTTTGTAGATCTGCATTTCAATTTGGTTTCTGCTAGAAGAGGGGGTGTTGAACCTCTTTATCTTCATGAGGACAATGAGTTGAAGGCTGAGGGTAATGATGTCAAGGATATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTTGCTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGCAAATCAAGGCATCGCTTGACTCCTGGAACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGGACTGCTCCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACATGTGATTCCGAGGCAGTACTTTCTGCAGCCAGGGAAAGAATCCAATTATTCAATAGTAGGATTTTTGATGTAGAAGGAGCTGATGAAGCTGAAAGAGATGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGGTTGATGATTTAGGCACTGAAATAAATCCAAAGATAGTAGATCAGAATTCTGAAGAAGCAAAAAAGTCAGTCAGACACAGAATATCTTTGGAAAGTAGAAAAGGAAGTTACGAGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAGAAGGAATTCCTTTAATCAATTCTGAAGCGTTCGTCAAGGTTGAAGACACTGGTTGTTCCTTGGATGATTCTGTTGACGCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAATCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCTTTAATTGGTGTGGCAATAGAAGGAAACTCTATTCGAATTGTTCTTGAGGAACGTTTAGAAGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGTGCAAGTTGATAAACGGCGTCTGAAAGAAGAACATGTCATCAAAATGCAATATACCCCTTTCATTGGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCTTCAGAAGATAGGCAAAGTCCATCTCCTACTGTTGATGAGAAAAACATTGAGACGACCGTGAAGCTTTCCGTCCTGCAAGAGCAGATGGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCTTCTGCAGGAAACCTACAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTGTTGCTGATAAGTCACACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCACTGCCCCTTGGTCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGTAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGTGCGTGGGTATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTCAAGGAAACTGTTAAAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATCAAAGACACTGCTAAAGCAGAAGATGTTGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACAGATAGCCCTACCAGCTCCGTTTCTGTTGCTGACTCTGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAACAAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGATCTGGAGAGGTGGATTTGGAAAGAATGTCTTGGTTCTTCAATATTATGTGCCGACAAAAAAGGGAAAAAAAGGTGTCCCCAGCTGCTGGACATCTGTAATTCTTGTCGTGAAATTTTTTATTTTGAAGAGGATCACTGTCATTCTTGCCATAGAACTTTTGGTAAGGGCGAAATAGATTTTTCACAACATGTGGTGCTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGACTTCTTCTCCCCTCAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAAATCTTGGGCTAGAAAGTTACATTTCTCATCATCAGCTGAAGACCTTCTCCAGGTTCTGGCATCTCTGGAGAGTGCTGTAAAGATAGAATATTTATCTTCAAACTTTGAGACTACTGGTGAGCTGCTATATACCTCAAATGAAAAAGGATGTGGTACTAGCGGTTCTTCTTCCCCTGATAGCGTTGCTGTGCTTCCATGGATACCACTTACAACAGCTGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCAGCCATCTCAAACATGCTACAGCAGAAGCTGGAGACTCAGAAGGACAAGGAATGTGGGGATTTCATAAAGCTTCCAACAAGATCTGCTGTCATGAAAAGTTCCCTGAATGAGGAGATCTCAGGAACTCCACATCAATCTGAAGATTCTAAAGAAGGCCATTGGGAGTATCTGGGGAGTGGACTTACTGGCTCTGGCCATGGAAGAGGCCGTAGTCGAACTCGTGCTGGGCGAGTGCCAAAAAAGGCAATTGGTTTGAGGCGCAATCCTAGGAAGAAGAGTAGAGTTACAGCCAAAGATAGATTAGGACAGGTGCAAGTACTGAAATGGAAAGGCAGACCACGTGGACGAGGAGGGCGAAAGCGTGGAAATCGGAGCATTAGAGGCAAGGAGACAATGTTTAACAGGATAATTAAGATTGGTGGGGATAGAACTGGCCCCAAAGACAGTGTCTATTATAAATCACCAGAGGACTTGGTTGAGGAGAATTGGAATGTAGGTGAAACTGCTGCAGAAAATGCAAGCGGTTCTGGAAGGTCAGAGTATGAAGTGGAAAATGGGGATGCGTCAGGAGATGAATTTGATCATGTGCATGTGGATGACTATGCCGGTGGCTTCAATGGCAAGTCTGATGACTTATTGGAGGGAGGCAAATATAACGTGGAGGGTGATGAAGATGATGTCGACGATGAGGTGGATGACGATGAAGGTGAAGGAGCACAAGATTCAAAAGGGGATTTTGATGGTGCAAGATATATCAATGGAGAGTGGGATGAAGAAGAACACATAGCTGATGATGGACAGCAAGACATGGAACTTGGTGAAGGTACCAGATCTTCGTCTTCAGACTATAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1652

Amino Acids

186.2

Weight (kDa)

5.52

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDT PF02791 445 - 500 1.2e-18 DDT domain
HARE-HTH PF05066 609 - 676 1.1e-17 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 827 - 870 3.7e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 1010 - 1083 1.6e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 4557
Acc65I GGTACC 1 cut(s) 4925
AccB1I GGYRCC 1 cut(s) 4925
AccB7I CCANNNNNTGG 2 cut(s) 3583, 3907
AccI GTMKAC 1 cut(s) 4782
AclI AACGTT 1 cut(s) 2614
AcoI YGGCCR 3 cut(s) 302, 1400, 4294
AcsI RAATTY 7 cut(s) 1007, 1418, 2230, 2340, 3609, 3718, 4676
AcvI CACGTG 1 cut(s) 4442
AcyI GRCGYC 2 cut(s) 41, 2677
AdeI CACNNNGTG 1 cut(s) 245
AflIII ACRYGT 3 cut(s) 2048, 2692, 3685
AjnI CCWGG 7 cut(s) 1563, 1822, 2078, 2311, 2479, 3106, 3900
Alw21I GWGCWC 4 cut(s) 682, 2032, 3428, 4819
Alw26I GTCTC 8 cut(s) 853, 1039, 1218, 2310, 2804, 3953, 4127, 4484
AlwNI CAGNNNCTG 5 cut(s) 2746, 3583, 3907, 4070, 4129
Ama87I CYCGRG 1 cut(s) 153
AoxI GGCC 8 cut(s) 206, 302, 1400, 1876, 4255, 4294, 4306, 4534
ApoI RAATTY 7 cut(s) 1007, 1418, 2230, 2340, 3609, 3718, 4676
ArsI GACNNNNNNTTYG 2 cut(s) 4533, 4565
AseI ATTAAT 1 cut(s) 690
Asp700I GAANNNNTTC 4 cut(s) 1422, 2340, 3819, 3894
Asp718I GGTACC 1 cut(s) 4925
AspA2I CCTAGG 1 cut(s) 4367
AspLEI GCGC 3 cut(s) 178, 2544, 4362
AspS9I GGNCC 6 cut(s) 28, 206, 1876, 2927, 3834, 4535
AsuC2I CCSGG 3 cut(s) 243, 2955, 3299
AsuII TTCGAA 1 cut(s) 2596
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 3 cut(s) 28, 2927, 3834
AvrII CCTAGG 1 cut(s) 4367
AxyI CCTNAGG 4 cut(s) 971, 2181, 2321, 3758
BalI TGGCCA 3 cut(s) 304, 1402, 4296
BamHI GGATCC 1 cut(s) 2176
BanI GGYRCC 1 cut(s) 4925
BanII GRGCYC 1 cut(s) 3428
BarI GAAGNNNNNNTAC 2 cut(s) 4918, 4950
BauI CACGAG 1 cut(s) 4320
BbrPI CACGTG 1 cut(s) 4442
BbsI GAAGAC 4 cut(s) 2385, 3251, 3897, 4932
Bbv12I GWGCWC 4 cut(s) 682, 2032, 3428, 4819
BbvCI CCTCAGC 1 cut(s) 1698
BceAI ACGGC 2 cut(s) 2690, 4293
BcgI CGANNNNNNTGC 2 cut(s) 3125, 3159
BciT130I CCWGG 7 cut(s) 1565, 1824, 2080, 2313, 2481, 3108, 3902
BciVI GTATCC 2 cut(s) 819, 4044
BclI TGATCA 2 cut(s) 2446, 4681
BcnI CCSGG 3 cut(s) 243, 2955, 3299
BcoDI GTCTC 8 cut(s) 853, 1039, 1218, 2310, 2804, 3953, 4127, 4484
BfaI CTAG 7 cut(s) 1449, 1644, 1977, 3449, 3861, 4008, 4368
BfmI CTRYAG 7 cut(s) 2035, 2073, 2415, 2892, 4118, 4608, 4948
BfoI RGCGCY 1 cut(s) 2545
BfuI GTATCC 2 cut(s) 819, 4044
BglII AGATCT 5 cut(s) 1618, 3496, 4181, 4215, 4931
BlnI CCTAGG 1 cut(s) 4367
BmcAI AGTACT 4 cut(s) 2067, 2519, 2918, 4419
Bme18I GGWCC 3 cut(s) 28, 2927, 3834
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 6 cut(s) 28, 206, 1876, 2927, 3834, 4535
BmiI GGNNCC 5 cut(s) 189, 256, 2178, 4537, 4927
BmrI ACTGGG 1 cut(s) 224
BmuI ACTGGG 1 cut(s) 224
BpiI GAAGAC 4 cut(s) 2385, 3251, 3897, 4932
BplI GAGNNNNNCTC 4 cut(s) 1990, 2022, 4202, 4234
BpmI CTGGAG 6 cut(s) 111, 2295, 3521, 3884, 3938, 4151
Bpu10I CCTNAGC 1 cut(s) 1698
Bpu14I TTCGAA 1 cut(s) 2596
BpuEI CTTGAG 2 cut(s) 1883, 2627
BpuMI CCSGG 3 cut(s) 243, 2955, 3299
Bsa29I ATCGAT 1 cut(s) 3031
BsaAI YACGTR 4 cut(s) 830, 2027, 3737, 4442
BsaBI GATNNNNATC 1 cut(s) 2475
BsaHI GRCGYC 2 cut(s) 41, 2677
BsaI GGTCTC 1 cut(s) 2310
BsaWI WCCGGW 2 cut(s) 15, 50
BsaXI ACNNNNNCTCC 6 cut(s) 1734, 1764, 3736, 3766, 4481, 4511
Bse118I RCCGGY 1 cut(s) 4706
Bse21I CCTNAGG 4 cut(s) 971, 2181, 2321, 3758
Bse3DI GCAATG 1 cut(s) 634
Bse8I GATNNNNATC 1 cut(s) 2475
BseBI CCWGG 7 cut(s) 1565, 1824, 2080, 2313, 2481, 3108, 3902
BseCI ATCGAT 1 cut(s) 3031
BseJI GATNNNNATC 1 cut(s) 2475
BseMI GCAATG 1 cut(s) 634
BseYI CCCAGC 3 cut(s) 181, 3576, 4325
BsgI GTGCAG 2 cut(s) 1307, 1536
Bsh1285I CGRYCG 2 cut(s) 2817, 3145
BshFI GGCC 8 cut(s) 208, 304, 1402, 1878, 4257, 4296, 4308, 4536
BshNI GGYRCC 1 cut(s) 4925
BshVI ATCGAT 1 cut(s) 3031
BsiEI CGRYCG 2 cut(s) 2817, 3145
BsiHKAI GWGCWC 4 cut(s) 682, 2032, 3428, 4819
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 7 cut(s) 16, 51, 77, 242, 2955, 3298, 4707
BslFI GGGAC 3 cut(s) 1209, 1994, 3558
BsmAI GTCTC 8 cut(s) 853, 1039, 1218, 2310, 2804, 3953, 4127, 4484
BsmBI CGTCTC 2 cut(s) 853, 2804
BsmFI GGGAC 3 cut(s) 1209, 1994, 3558
BsmI GAATGC 2 cut(s) 1163, 3289
BsnI GGCC 8 cut(s) 208, 304, 1402, 1878, 4257, 4296, 4308, 4536
Bso31I GGTCTC 1 cut(s) 2310
BsoBI CYCGRG 1 cut(s) 153
Bsp119I TTCGAA 1 cut(s) 2596
Bsp1286I GDGCHC 4 cut(s) 682, 2032, 3428, 4819
Bsp19I CCATGG 4 cut(s) 660, 2489, 4046, 4297
BspANI GGCC 8 cut(s) 208, 304, 1402, 1878, 4257, 4296, 4308, 4536
BspDI ATCGAT 1 cut(s) 3031
BspHI TCATGA 5 cut(s) 736, 1435, 1675, 2443, 4191
BspLI GGNNCC 5 cut(s) 189, 256, 2178, 4537, 4927
BspMAI CTGCAG 4 cut(s) 2077, 2419, 2896, 4612
BspQI GCTCTTC 1 cut(s) 3825
BspT104I TTCGAA 1 cut(s) 2596
BspT107I GGYRCC 1 cut(s) 4925
BspTNI GGTCTC 1 cut(s) 2310
BsrDI GCAATG 1 cut(s) 634
BsrFI RCCGGY 1 cut(s) 4706
BssAI RCCGGY 1 cut(s) 4706
BssNI GRCGYC 2 cut(s) 41, 2677
BssSI CACGAG 1 cut(s) 4320
BssT1I CCWWGG 8 cut(s) 660, 1316, 2394, 2489, 3043, 4046, 4297, 4367
Bst2BI CACGAG 1 cut(s) 4320
Bst2UI CCWGG 7 cut(s) 1565, 1824, 2080, 2313, 2481, 3108, 3902
Bst6I CTCTTC 7 cut(s) 918, 1642, 3162, 3618, 3825, 4297, 4370
BstACI GRCGYC 2 cut(s) 41, 2677
BstAPI GCANNNNNTGC 2 cut(s) 2558, 4616
BstBAI YACGTR 4 cut(s) 830, 2027, 3737, 4442
BstBI TTCGAA 1 cut(s) 2596
BstC8I GCNNGC 1 cut(s) 4621
BstDSI CCRYGG 4 cut(s) 660, 2489, 4046, 4297
BstEII GGTNACC 1 cut(s) 1572
BstENI CCTNNNNNAGG 4 cut(s) 2892, 3047, 3899, 4208
BstH2I RGCGCY 1 cut(s) 2545
BstHHI GCGC 3 cut(s) 178, 2544, 4362
BstMAI GTCTC 8 cut(s) 853, 1039, 1218, 2310, 2804, 3953, 4127, 4484
BstMCI CGRYCG 2 cut(s) 2817, 3145
BstNI CCWGG 7 cut(s) 1565, 1824, 2080, 2313, 2481, 3108, 3902
BstNSI RCATGY 5 cut(s) 2052, 2696, 3689, 4117, 4694
BstPI GGTNACC 1 cut(s) 1572
BstSFI CTRYAG 7 cut(s) 2035, 2073, 2415, 2892, 4118, 4608, 4948
BstSNI TACGTA 1 cut(s) 830
BstV2I GAAGAC 4 cut(s) 2385, 3251, 3897, 4932
BstX2I RGATCY 9 cut(s) 1484, 1618, 1927, 2176, 3120, 3496, 4181, 4215, 4931
BstXI CCANNNNNNTGG 2 cut(s) 557, 730
BstYI RGATCY 9 cut(s) 1484, 1618, 1927, 2176, 3120, 3496, 4181, 4215, 4931
Bsu15I ATCGAT 1 cut(s) 3031
Bsu36I CCTNAGG 4 cut(s) 971, 2181, 2321, 3758
BsuI GTATCC 2 cut(s) 819, 4044
BsuRI GGCC 8 cut(s) 208, 304, 1402, 1878, 4257, 4296, 4308, 4536
BsuTUI ATCGAT 1 cut(s) 3031
BtgI CCRYGG 4 cut(s) 660, 2489, 4046, 4297
BtgZI GCGATG 1 cut(s) 1796
BtsI GCAGTG 7 cut(s) 1553, 1602, 1744, 2661, 3035, 3318, 3370
Cac8I GCNNGC 1 cut(s) 4621
CaiI CAGNNNCTG 5 cut(s) 2746, 3583, 3907, 4070, 4129
CciI TCATGA 5 cut(s) 736, 1435, 1675, 2443, 4191
CfoI GCGC 3 cut(s) 178, 2544, 4362
Cfr10I RCCGGY 1 cut(s) 4706
Cfr13I GGNCC 6 cut(s) 28, 206, 1876, 2927, 3834, 4535
ClaI ATCGAT 1 cut(s) 3031
CseI GACGC 4 cut(s) 49, 2420, 2666, 4653
CspCI CAANNNNNGTGG 2 cut(s) 4135, 4170
DraI TTTAAA 2 cut(s) 2637, 3483
DraIII CACNNNGTG 1 cut(s) 245
EaeI YGGCCR 3 cut(s) 302, 1400, 4294
Eam1104I CTCTTC 7 cut(s) 918, 1642, 3162, 3618, 3825, 4297, 4370
EarI CTCTTC 7 cut(s) 918, 1642, 3162, 3618, 3825, 4297, 4370
Ecl136II GAGCTC 1 cut(s) 3426
Eco105I TACGTA 1 cut(s) 830
Eco130I CCWWGG 8 cut(s) 660, 1316, 2394, 2489, 3043, 4046, 4297, 4367
Eco24I GRGCYC 1 cut(s) 3428
Eco31I GGTCTC 1 cut(s) 2310
Eco32I GATATC 2 cut(s) 853, 3491
Eco47I GGWCC 3 cut(s) 28, 2927, 3834
Eco53kI GAGCTC 1 cut(s) 3426
Eco72I CACGTG 1 cut(s) 4442
Eco81I CCTNAGG 4 cut(s) 971, 2181, 2321, 3758
Eco88I CYCGRG 1 cut(s) 153
Eco91I GGTNACC 1 cut(s) 1572
EcoICRI GAGCTC 1 cut(s) 3426
EcoNI CCTNNNNNAGG 4 cut(s) 2892, 3047, 3899, 4208
EcoO109I RGGNCCY 1 cut(s) 1876
EcoO65I GGTNACC 1 cut(s) 1572
EcoRI GAATTC 4 cut(s) 1418, 2230, 2340, 3718
EcoRII CCWGG 7 cut(s) 1563, 1822, 2078, 2311, 2479, 3106, 3900
EcoRV GATATC 2 cut(s) 853, 3491
EcoT14I CCWWGG 8 cut(s) 660, 1316, 2394, 2489, 3043, 4046, 4297, 4367
EcoT38I GRGCYC 1 cut(s) 3428
ErhI CCWWGG 8 cut(s) 660, 1316, 2394, 2489, 3043, 4046, 4297, 4367
Esp3I CGTCTC 2 cut(s) 853, 2804
FalI AAGNNNNNCTT 2 cut(s) 3510, 3542
FaqI GGGAC 3 cut(s) 1209, 1994, 3558
FauI CCCGC 2 cut(s) 106, 792
FauNDI CATATG 1 cut(s) 307
FbaI TGATCA 2 cut(s) 2446, 4681
FblI GTMKAC 1 cut(s) 4782
FriOI GRGCYC 1 cut(s) 3428
FspBI CTAG 7 cut(s) 1449, 1644, 1977, 3449, 3861, 4008, 4368
GlaI GCGC 3 cut(s) 177, 2543, 4361
GsaI CCCAGC 3 cut(s) 185, 3580, 4329
GsuI CTGGAG 6 cut(s) 111, 2295, 3521, 3884, 3938, 4151
HaeII RGCGCY 1 cut(s) 2545
HaeIII GGCC 8 cut(s) 208, 304, 1402, 1878, 4257, 4296, 4308, 4536
HapII CCGG 7 cut(s) 16, 51, 77, 242, 2955, 3298, 4707
HgaI GACGC 4 cut(s) 49, 2420, 2666, 4653
HhaI GCGC 3 cut(s) 178, 2544, 4362
Hin1I GRCGYC 2 cut(s) 41, 2677
Hin6I GCGC 3 cut(s) 176, 2542, 4360
HinP1I GCGC 3 cut(s) 176, 2542, 4360
HincII GTYRAC 2 cut(s) 2410, 4783
HindII GTYRAC 2 cut(s) 2410, 4783
HindIII AAGCTT 3 cut(s) 1428, 2822, 4169
HpaII CCGG 7 cut(s) 16, 51, 77, 242, 2955, 3298, 4707
Hpy166II GTNNAC 7 cut(s) 452, 989, 2024, 2410, 4280, 4445, 4783
Hpy8I GTNNAC 7 cut(s) 452, 989, 2024, 2410, 4280, 4445, 4783
Hpy99I CGWCG 2 cut(s) 43, 4787
HpyCH4IV ACGT 6 cut(s) 829, 2026, 2614, 3736, 4441, 4758
HpySE526I ACGT 6 cut(s) 829, 2026, 2614, 3736, 4441, 4758
Hsp92I GRCGYC 2 cut(s) 41, 2677
HspAI GCGC 3 cut(s) 176, 2542, 4360
KpnI GGTACC 1 cut(s) 4929
Ksp22I TGATCA 2 cut(s) 2446, 4681
LguI GCTCTTC 1 cut(s) 3825
MaeI CTAG 7 cut(s) 1449, 1644, 1977, 3449, 3861, 4008, 4368
MaeII ACGT 6 cut(s) 829, 2026, 2614, 3736, 4441, 4758
MaeIII GTNAC 8 cut(s) 1572, 2291, 2329, 2973, 3002, 3867, 4384, 4952
MfeI CAATTG 2 cut(s) 3441, 4347
MflI RGATCY 9 cut(s) 1484, 1618, 1927, 2176, 3120, 3496, 4181, 4215, 4931
MhlI GDGCHC 4 cut(s) 682, 2032, 3428, 4819
MlsI TGGCCA 3 cut(s) 304, 1402, 4296
MluNI TGGCCA 3 cut(s) 304, 1402, 4296
MlyI GAGTC 6 cut(s) 65, 1031, 1813, 1976, 3401, 4129
MmeI TCCRAC 3 cut(s) 3128, 3399, 4199
Mox20I TGGCCA 3 cut(s) 304, 1402, 4296
MroXI GAANNNNTTC 4 cut(s) 1422, 2340, 3819, 3894
MscI TGGCCA 3 cut(s) 304, 1402, 4296
MslI CAYNNNNRTG 6 cut(s) 444, 513, 1440, 2702, 4689, 4695
Msp20I TGGCCA 3 cut(s) 304, 1402, 4296
MspA1I CMGCKG 4 cut(s) 181, 3580, 3887, 4067
MspI CCGG 7 cut(s) 16, 51, 77, 242, 2955, 3298, 4707
MunI CAATTG 2 cut(s) 3441, 4347
Mva1269I GAATGC 2 cut(s) 1163, 3289
MvaI CCWGG 7 cut(s) 1565, 1824, 2080, 2313, 2481, 3108, 3902
NciI CCSGG 3 cut(s) 243, 2955, 3299
NcoI CCATGG 4 cut(s) 660, 2489, 4046, 4297
NdeI CATATG 1 cut(s) 307
NlaIV GGNNCC 5 cut(s) 189, 256, 2178, 4537, 4927
NmuCI GTSAC 5 cut(s) 1572, 2329, 2973, 3002, 4952
NspI RCATGY 5 cut(s) 2052, 2696, 3689, 4117, 4694
NspV TTCGAA 1 cut(s) 2596
PaeR7I CTCGAG 1 cut(s) 153
PagI TCATGA 5 cut(s) 736, 1435, 1675, 2443, 4191
PciI ACATGT 3 cut(s) 2048, 2692, 3685
PciSI GCTCTTC 1 cut(s) 3825
PcsI WCGNNNNNNNCGW 3 cut(s) 1419, 4327, 4453
PctI GAATGC 2 cut(s) 1163, 3289
PdmI GAANNNNTTC 4 cut(s) 1422, 2340, 3819, 3894
PflFI GACNNNGTC 1 cut(s) 4547
PflMI CCANNNNNTGG 2 cut(s) 3583, 3907
PfoI TCCNGGA 2 cut(s) 1822, 2311
PleI GAGTC 6 cut(s) 65, 1030, 1813, 1976, 3401, 4129
PmaCI CACGTG 1 cut(s) 4442
PmlI CACGTG 1 cut(s) 4442
PpsI GAGTC 6 cut(s) 65, 1030, 1813, 1976, 3401, 4129
Ppu21I YACGTR 4 cut(s) 830, 2027, 3737, 4442
PscI ACATGT 3 cut(s) 2048, 2692, 3685
PshBI ATTAAT 1 cut(s) 690
PsiI TTATAA 1 cut(s) 4557
Psp124BI GAGCTC 1 cut(s) 3428
Psp1406I AACGTT 1 cut(s) 2614
Psp6I CCWGG 7 cut(s) 1563, 1822, 2078, 2311, 2479, 3106, 3900
PspCI CACGTG 1 cut(s) 4442
PspEI GGTNACC 1 cut(s) 1572
PspFI CCCAGC 3 cut(s) 181, 3576, 4325
PspGI CCWGG 7 cut(s) 1563, 1822, 2078, 2311, 2479, 3106, 3900
PspN4I GGNNCC 5 cut(s) 189, 256, 2178, 4537, 4927
PspPI GGNCC 6 cut(s) 28, 206, 1876, 2927, 3834, 4535
PspXI VCTCGAGB 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 3997, 4029
PstI CTGCAG 4 cut(s) 2077, 2419, 2896, 4612
PstNI CAGNNNCTG 5 cut(s) 2746, 3583, 3907, 4070, 4129
PsuI RGATCY 9 cut(s) 1484, 1618, 1927, 2176, 3120, 3496, 4181, 4215, 4931
PsyI GACNNNGTC 1 cut(s) 4547
PvuII CAGCTG 4 cut(s) 181, 3580, 3887, 4067
RseI CAYNNNNRTG 6 cut(s) 444, 513, 1440, 2702, 4689, 4695
SacI GAGCTC 1 cut(s) 3428
SalI GTCGAC 1 cut(s) 4781
SapI GCTCTTC 1 cut(s) 3825
Sau96I GGNCC 6 cut(s) 28, 206, 1876, 2927, 3834, 4535
ScaI AGTACT 4 cut(s) 2067, 2519, 2918, 4419
SchI GAGTC 6 cut(s) 65, 1031, 1813, 1976, 3401, 4129
SduI GDGCHC 4 cut(s) 682, 2032, 3428, 4819
SfcI CTRYAG 7 cut(s) 2035, 2073, 2415, 2892, 4118, 4608, 4948
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 1 cut(s) 2596
SinI GGWCC 3 cut(s) 28, 2927, 3834
SlaI CTCGAG 1 cut(s) 153
SmiMI CAYNNNNRTG 6 cut(s) 444, 513, 1440, 2702, 4689, 4695
SmlI CTYRAG 3 cut(s) 153, 1862, 2606
SmoI CTYRAG 3 cut(s) 153, 1862, 2606
SnaBI TACGTA 1 cut(s) 830
SspI AATATT 4 cut(s) 655, 3255, 3540, 3941
SspMI CTAG 7 cut(s) 1449, 1644, 1977, 3449, 3861, 4008, 4368
SstI GAGCTC 1 cut(s) 3428
StyI CCWWGG 8 cut(s) 660, 1316, 2394, 2489, 3043, 4046, 4297, 4367
TaiI ACGT 6 cut(s) 832, 2029, 2617, 3739, 4444, 4761
TaqI TCGA 9 cut(s) 154, 1413, 1958, 2596, 3031, 3097, 3743, 4315, 4782
TaqII GACCGA 1 cut(s) 3851
TatI WGTACW 5 cut(s) 1265, 2065, 2517, 2916, 4417
TauI GCSGC 4 cut(s) 61, 127, 130, 133
TseFI GTSAC 5 cut(s) 1572, 2329, 2973, 3002, 4952
Tsp45I GTSAC 5 cut(s) 1572, 2329, 2973, 3002, 4952
TspGWI ACGGA 4 cut(s) 222, 250, 2818, 3382
Tth111I GACNNNGTC 1 cut(s) 4547
Van91I CCANNNNNTGG 2 cut(s) 3583, 3907
VpaK11BI GGWCC 3 cut(s) 28, 2927, 3834
VspI ATTAAT 1 cut(s) 690
XagI CCTNNNNNAGG 4 cut(s) 2892, 3047, 3899, 4208
XapI RAATTY 7 cut(s) 1007, 1418, 2230, 2340, 3609, 3718, 4676
XceI RCATGY 5 cut(s) 2052, 2696, 3689, 4117, 4694
XcmI CCANNNNNNNNNTGG 1 cut(s) 3653
XhoI CTCGAG 1 cut(s) 153
XmaJI CCTAGG 1 cut(s) 4367
XmiI GTMKAC 1 cut(s) 4782
XmnI GAANNNNTTC 4 cut(s) 1422, 2340, 3819, 3894
XspI CTAG 7 cut(s) 1449, 1644, 1977, 3449, 3861, 4008, 4368
ZrmI AGTACT 4 cut(s) 2067, 2519, 2918, 4419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.