Rh2DG355100

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0002907
Physical Location & Seq
Reverse (-)
49767012 .. 49781666
14655 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG355100.1

Sequence Viewer

Length: 3738 bp
ATGCGGAGTACATTCCCACCCAAATCTGTGCAGTTGAAAAAACCTTTTGGTATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGGCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATTCATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAATACCCTCTATGGGGGTGGGAGCTAATCAGAACTGCGCTGCAAATCCTGGAGGCGGTCACCCTCAAATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACTTAAATCCACTGACATGGCCAGAAATATTGCGGCAATTTTCTGTATCAGCAGGATTTGGACCGCAACTGAAGAAGAGGGATGTTGAACCAGCTTATCTTCATGAGGATAATGAGGGTAATGATGTCAAGGATATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTCGCTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGCAAATCAAGGCATTGCTTGACTCCTGGCACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGAACTGCACCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACAAGTGATTCCGAGGCAGTACTTTCGGCAGCCAGAGAAAGAATTCAAATATTCAATAGTAGGATTTTTGATGTTGAAGGAGCTGATGAAGCTGAAAGAGACGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGTTTGATGATTTAGGCACTGAAATAAATCCAAAGATAGTAGCTCAGAATTCTGAAGAAGCAAGAAAAGTCAGTGACGGAATATCTTTGGAAAGTGGAAAAGGAAGTTACGAGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAGAAGGAATTCCTTCAATCAATTCTGAAGCGTCCATCAAGGTTGAAGACGCTGGTTGTTCCTTGGATTCTGTTGACGCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAACCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCATTAATTGGTGTGGCAATAGAAGGAAACTCGATTCGAATTGTTCTCGAGGAACGTTTAGATGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGCGCAAGTTGATAAACGGCGTCTAAAAGAAGAACATGTCATCAAAATGCAATATACCCCTTTCATTGGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCAGAAAATAGGCAAAGTCCATCTCCTACCGTTGATGAGAAAAACATTGAGACGACGGTGAAGCTTTCCGTCCTGCAAGAGCAGATGGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCTTCTGTACGAAACCTCCAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTATTGCTGATAAGTCGCACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCATTGCCTCTTGGTCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGCAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGTGCGTGGGTATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTCAAGGAAACTGTTAGAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATCAAAGACACTGCTAAAACAGAAGATGATGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACGGATAGCCCTACCAGCTCTGTTTCTGTTGCTGACTCCGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAACGAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGATCTGGAGAGGTGGATTTTGAAAGAAAGTGTTGGTTCTTCAATATTATGTGCTGACAAAAATGGGGAAAAAAGGTGTCCCCAGCTACTGGACATCTGCAATTCTTGTCGTGGAATTTTTTATTTTGAAGAGAATCACTGTCATTCTTGCCATAGAACTTTTGGCAAGGGCGAAATAGATTTTTCACAACATGTGGTACTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGAGTTCTTCTCCCCTTAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAAATCTTGGGGTAGAAAGTTACATTCCTCGTCAACAGCTGAAGACCTTCTCCAGGTTCTGGCATCGTTGGAGAGTGCTGTAAAGATAGAATATTTATCTTCAAACTTTGAGACTACCGGTGAGCTGCTATATTCCTCAAATGAAAAAGGACGTGTTACTAGCAATTCTTCTTCTCCTGATAGCGTTGCTGTGCTTCCATGGGTACCACTTACAACAGCTGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCGGCCATCTCAAACGTGCTACAGCAGAAGCTGGTGACTCAGAAGGACAAGGAATCTGGGGATTTTAAAAAGCTTCCAACAAGATCTGCTGTCATGAAAAGTTCCAAGAATGAGGAGATCTCAGGAACTCCACATCAATCTGAAGATTCTAAAGAAGGCCACTGGGAGTATCTGGGGAGTGGACTTACCGGCTCTGGCCATGGACGAGGCCGTGGTCGAACTGGTGGTAGGCGAGTGCCAAAAAAGGCAATTGGTTTAAGGGGCAATCCTAGGAAGAAGAGTAGAGTTACAGCCAAAGATAAATTAGTACAGGTGCAAGTACTGAAATGGAAAGGCAGACCACGTGGACGAGGAGGGCGAAAGCGTGGAAATCGGAGCATTAGAGGCAAGGAGACAACGTTTAACAGGATAATTAAGATTGGTGGGGAGAGAACTGGCCCCAAAGACAGTGTCTATTATAAATCACCAGATGACTTGGTTGAGGAGAATTGGAACGTAGGTGAAACTGCGGCAGAAAATGCAAGCGGTTCTGGAAGGTCAGAGTATGAAGTGGAAAATGGGGATGCATCAGGAGATGAATTTGATCATGTGCATGTGGATGACTATGCCGGTGGCTTCAATGGCAAGTCTGATGACTTATTGGAGGGAGGCGAATATAACATGGAGGGTGACGAAGATGATGTCGATGATGAGGTGGATGACGATGAAGGTGAAGGAGCACAGGATTCGGATTTTGATGGTGCAAGATATATCAATGGAGAGTGGGATGAAGAGGAACACATAGCTGATGATGGACAGCAAGACATGGAACTTGGTGAAGGTACCAGATCTTCGTCTTCAGACTATAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1245

Amino Acids

138.36

Weight (kDa)

5.1

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDT PF02791 26 - 81 8.7e-19 DDT domain
HARE-HTH PF05066 207 - 274 8.1e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 423 - 466 2.7e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 605 - 678 1.1e-21 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3342
Acc65I GGTACC 2 cut(s) 2836, 3704
AccB1I GGYRCC 2 cut(s) 2836, 3704
AccB7I CCANNNNNTGG 2 cut(s) 2368, 2692
AciI CCGC 7 cut(s) 4, 315, 412, 443, 2885, 3392, 3408
AclI AACGTT 2 cut(s) 1402, 3281
AclWI GGATC 9 cut(s) 222, 729, 965, 978, 1631, 1845, 1883, 1913, 2555
AcoI YGGCCR 4 cut(s) 143, 398, 2886, 3079
AcsI RAATTY 7 cut(s) 161, 882, 1024, 1131, 2394, 2503, 3461
AcvI CACGTG 1 cut(s) 3227
AcyI GRCGYC 1 cut(s) 1465
AfiI CCNNNNNNNGG 9 cut(s) 272, 273, 314, 1511, 1696, 1898, 2368, 2686, 2692
AflIII ACRYGT 3 cut(s) 1480, 2470, 2785
AgeI ACCGGT 1 cut(s) 2750
AjiI CACGTC 1 cut(s) 2786
AjnI CCWGG 6 cut(s) 307, 616, 1102, 1267, 1891, 2685
Alw21I GWGCWC 3 cut(s) 826, 2213, 3604
Alw26I GTCTC 5 cut(s) 933, 1101, 1589, 2738, 3269
AlwI GGATC 9 cut(s) 222, 729, 965, 978, 1631, 1845, 1883, 1913, 2555
AlwNI CAGNNNCTG 5 cut(s) 1534, 2368, 2692, 2855, 2914
Ama87I CYCGRG 2 cut(s) 1394, 2527
AoxI GGCC 8 cut(s) 143, 398, 670, 2886, 3040, 3079, 3091, 3319
ApoI RAATTY 7 cut(s) 161, 882, 1024, 1131, 2394, 2503, 3461
ArsI GACNNNNNNTTYG 4 cut(s) 3318, 3350, 3549, 3581
AseI ATTAAT 1 cut(s) 1352
AsiGI ACCGGT 1 cut(s) 2750
Asp700I GAANNNNTTC 6 cut(s) 165, 882, 1131, 1135, 2604, 2679
Asp718I GGTACC 2 cut(s) 2836, 3704
AspA2I CCTAGG 1 cut(s) 3152
AspLEI GCGC 3 cut(s) 299, 1332, 1450
AspS9I GGNCC 5 cut(s) 440, 670, 1712, 2619, 3320
AsuC2I CCSGG 2 cut(s) 1740, 2084
AsuII TTCGAA 1 cut(s) 1384
AvaI CYCGRG 2 cut(s) 1394, 2527
AvaII GGWCC 3 cut(s) 440, 1712, 2619
AvrII CCTAGG 1 cut(s) 3152
AxyI CCTNAGG 2 cut(s) 1112, 2543
BalI TGGCCA 3 cut(s) 145, 400, 3081
BamHI GGATCC 1 cut(s) 970
BanI GGYRCC 2 cut(s) 2836, 3704
BanII GRGCYC 1 cut(s) 2213
BarI GAAGNNNNNNTAC 2 cut(s) 3697, 3729
BbrPI CACGTG 1 cut(s) 3227
BbsI GAAGAC 4 cut(s) 1176, 2036, 2682, 3711
Bbv12I GWGCWC 3 cut(s) 826, 2213, 3604
BccI CCATC 8 cut(s) 239, 1166, 1573, 1624, 1958, 2897, 3614, 3668
BceAI ACGGC 2 cut(s) 1478, 3078
BcgI CGANNNNNNTGC 6 cut(s) 846, 880, 1910, 1944, 3591, 3625
BciT130I CCWGG 6 cut(s) 309, 618, 1104, 1269, 1893, 2687
BclI TGATCA 2 cut(s) 1234, 3466
BcnI CCSGG 2 cut(s) 1740, 2084
BcoDI GTCTC 5 cut(s) 933, 1101, 1589, 2738, 3269
BfaI CTAG 5 cut(s) 192, 771, 2234, 2793, 3153
BfmI CTRYAG 4 cut(s) 829, 1203, 2903, 3727
BfoI RGCGCY 1 cut(s) 1333
BglII AGATCT 4 cut(s) 2281, 2966, 3000, 3710
BlnI CCTAGG 1 cut(s) 3152
BmcAI AGTACT 4 cut(s) 861, 1307, 1703, 3204
Bme1390I CCNGG 8 cut(s) 309, 618, 1104, 1269, 1740, 1893, 2084, 2687
Bme18I GGWCC 3 cut(s) 440, 1712, 2619
BmeT110I CYCGRG 2 cut(s) 1394, 2527
BmgBI CACGTC 1 cut(s) 2786
BmgT120I GGNCC 5 cut(s) 440, 670, 1712, 2619, 3320
BmiI GGNNCC 4 cut(s) 972, 2838, 3322, 3706
BmrFI CCNGG 8 cut(s) 309, 618, 1104, 1269, 1740, 1893, 2084, 2687
BmrI ACTGGG 1 cut(s) 3055
BmsI GCATC 7 cut(s) 949, 1399, 1985, 2705, 2872, 3436, 3458
BmuI ACTGGG 1 cut(s) 3055
BpiI GAAGAC 4 cut(s) 1176, 2036, 2682, 3711
BplI GAGNNNNNCTC 4 cut(s) 2521, 2553, 2987, 3019
BpmI CTGGAG 4 cut(s) 330, 1086, 2306, 2669
Bpu14I TTCGAA 1 cut(s) 1384
BpuEI CTTGAG 1 cut(s) 677
BpuMI CCSGG 2 cut(s) 1740, 2084
Bsa29I ATCGAT 1 cut(s) 1816
BsaAI YACGTR 3 cut(s) 821, 2522, 3227
BsaHI GRCGYC 1 cut(s) 1465
BsaI GGTCTC 1 cut(s) 1101
BsaWI WCCGGW 1 cut(s) 2750
BsaXI ACNNNNNCTCC 6 cut(s) 528, 558, 1671, 1701, 3266, 3296
Bsc4I CCNNNNNNNGG 9 cut(s) 272, 273, 314, 1511, 1696, 1898, 2368, 2686, 2692
Bse118I RCCGGY 3 cut(s) 2750, 3071, 3491
Bse1I ACTGG 6 cut(s) 1862, 2263, 2373, 3050, 3109, 3322
Bse21I CCTNAGG 2 cut(s) 1112, 2543
Bse3DI GCAATG 2 cut(s) 604, 1820
BseBI CCWGG 6 cut(s) 309, 618, 1104, 1269, 1893, 2687
BseCI ATCGAT 1 cut(s) 1816
BseGI GGATG 7 cut(s) 133, 466, 1990, 3451, 3487, 3586, 3655
BseLI CCNNNNNNNGG 9 cut(s) 272, 273, 314, 1511, 1696, 1898, 2368, 2686, 2692
BseMI GCAATG 2 cut(s) 604, 1820
BseMII CTCAG 7 cut(s) 966, 1034, 1515, 2241, 2571, 2936, 3018
BseNI ACTGG 6 cut(s) 1862, 2263, 2373, 3050, 3109, 3322
BseRI GAGGAG 6 cut(s) 89, 1340, 1814, 3011, 3249, 3380
BseYI CCCAGC 1 cut(s) 2361
BsgI GTGCAG 2 cut(s) 50, 783
Bsh1285I CGRYCG 1 cut(s) 1930
BshFI GGCC 8 cut(s) 145, 400, 672, 2888, 3042, 3081, 3093, 3321
BshNI GGYRCC 2 cut(s) 2836, 3704
BshTI ACCGGT 1 cut(s) 2750
BshVI ATCGAT 1 cut(s) 1816
BsiEI CGRYCG 1 cut(s) 1930
BsiHKAI GWGCWC 3 cut(s) 826, 2213, 3604
BsiHKCI CYCGRG 2 cut(s) 1394, 2527
BsiSI CCGG 5 cut(s) 1740, 2083, 2751, 3072, 3492
BslFI GGGAC 2 cut(s) 788, 2343
BslI CCNNNNNNNGG 9 cut(s) 272, 273, 314, 1511, 1696, 1898, 2368, 2686, 2692
BsmAI GTCTC 5 cut(s) 933, 1101, 1589, 2738, 3269
BsmBI CGTCTC 2 cut(s) 933, 1589
BsmFI GGGAC 2 cut(s) 788, 2343
BsmI GAATGC 1 cut(s) 2074
BsnI GGCC 8 cut(s) 145, 400, 672, 2888, 3042, 3081, 3093, 3321
Bso31I GGTCTC 1 cut(s) 1101
BsoBI CYCGRG 2 cut(s) 1394, 2527
Bsp119I TTCGAA 1 cut(s) 1384
Bsp1286I GDGCHC 3 cut(s) 826, 2213, 3604
Bsp19I CCATGG 3 cut(s) 1277, 2831, 3082
BspACI CCGC 7 cut(s) 4, 315, 412, 443, 2885, 3392, 3408
BspANI GGCC 8 cut(s) 145, 400, 672, 2888, 3042, 3081, 3093, 3321
BspCNI CTCAG 7 cut(s) 967, 1033, 1516, 2242, 2572, 2935, 3017
BspDI ATCGAT 1 cut(s) 1816
BspHI TCATGA 4 cut(s) 178, 481, 1231, 2976
BspLI GGNNCC 4 cut(s) 972, 2838, 3322, 3706
BspMAI CTGCAG 1 cut(s) 1207
BspPI GGATC 9 cut(s) 222, 729, 965, 978, 1631, 1845, 1883, 1913, 2555
BspQI GCTCTTC 1 cut(s) 2610
BspT104I TTCGAA 1 cut(s) 1384
BspT107I GGYRCC 2 cut(s) 2836, 3704
BspTNI GGTCTC 1 cut(s) 1101
BsrDI GCAATG 2 cut(s) 604, 1820
BsrFI RCCGGY 3 cut(s) 2750, 3071, 3491
BsrI ACTGG 6 cut(s) 1862, 2263, 2373, 3050, 3109, 3322
BssAI RCCGGY 3 cut(s) 2750, 3071, 3491
BssNI GRCGYC 1 cut(s) 1465
BssT1I CCWWGG 6 cut(s) 59, 1185, 1277, 2831, 3082, 3152
Bst2UI CCWGG 6 cut(s) 309, 618, 1104, 1269, 1893, 2687
Bst6I CTCTTC 6 cut(s) 449, 1947, 2403, 2610, 3155, 3648
BstACI GRCGYC 1 cut(s) 1465
BstAPI GCANNNNNTGC 2 cut(s) 1346, 3401
BstBAI YACGTR 3 cut(s) 821, 2522, 3227
BstBI TTCGAA 1 cut(s) 1384
BstC8I GCNNGC 1 cut(s) 3406
BstDSI CCRYGG 4 cut(s) 1277, 2831, 3082, 3094
BstEII GGTNACC 1 cut(s) 317
BstENI CCTNNNNNAGG 1 cut(s) 2684
BstF5I GGATG 7 cut(s) 133, 466, 1990, 3451, 3487, 3586, 3655
BstH2I RGCGCY 1 cut(s) 1333
BstHHI GCGC 3 cut(s) 299, 1332, 1450
BstMAI GTCTC 5 cut(s) 933, 1101, 1589, 2738, 3269
BstMCI CGRYCG 1 cut(s) 1930
BstNI CCWGG 6 cut(s) 309, 618, 1104, 1269, 1893, 2687
BstNSI RCATGY 3 cut(s) 1484, 2474, 3479
BstPI GGTNACC 1 cut(s) 317
BstSCI CCNGG 8 cut(s) 307, 616, 1102, 1267, 1738, 1891, 2082, 2685
BstSFI CTRYAG 4 cut(s) 829, 1203, 2903, 3727
BstV2I GAAGAC 4 cut(s) 1176, 2036, 2682, 3711
BstX2I RGATCY 8 cut(s) 227, 721, 970, 1905, 2281, 2966, 3000, 3710
BstXI CCANNNNNNTGG 1 cut(s) 396
BstYI RGATCY 8 cut(s) 227, 721, 970, 1905, 2281, 2966, 3000, 3710
Bsu15I ATCGAT 1 cut(s) 1816
Bsu36I CCTNAGG 2 cut(s) 1112, 2543
BsuRI GGCC 8 cut(s) 145, 400, 672, 2888, 3042, 3081, 3093, 3321
BsuTUI ATCGAT 1 cut(s) 1816
BtgI CCRYGG 4 cut(s) 1277, 2831, 3082, 3094
BtrI CACGTC 1 cut(s) 2786
BtsCI GGATG 7 cut(s) 133, 466, 1990, 3451, 3487, 3586, 3655
BtsI GCAGTG 4 cut(s) 538, 1449, 2103, 2155
Cac8I GCNNGC 1 cut(s) 3406
CaiI CAGNNNCTG 5 cut(s) 1534, 2368, 2692, 2855, 2914
CciI TCATGA 4 cut(s) 178, 481, 1231, 2976
CfoI GCGC 3 cut(s) 299, 1332, 1450
Cfr10I RCCGGY 3 cut(s) 2750, 3071, 3491
Cfr13I GGNCC 5 cut(s) 440, 670, 1712, 2619, 3320
ClaI ATCGAT 1 cut(s) 1816
CseI GACGC 4 cut(s) 1143, 1181, 1208, 1454
CspAI ACCGGT 1 cut(s) 2750
DraI TTTAAA 3 cut(s) 1425, 2268, 2950
EaeI YGGCCR 4 cut(s) 143, 398, 2886, 3079
Eam1104I CTCTTC 6 cut(s) 449, 1947, 2403, 2610, 3155, 3648
EarI CTCTTC 6 cut(s) 449, 1947, 2403, 2610, 3155, 3648
Ecl136II GAGCTC 1 cut(s) 2211
Eco130I CCWWGG 6 cut(s) 59, 1185, 1277, 2831, 3082, 3152
Eco24I GRGCYC 1 cut(s) 2213
Eco31I GGTCTC 1 cut(s) 1101
Eco32I GATATC 1 cut(s) 2276
Eco47I GGWCC 3 cut(s) 440, 1712, 2619
Eco53kI GAGCTC 1 cut(s) 2211
Eco72I CACGTG 1 cut(s) 3227
Eco81I CCTNAGG 2 cut(s) 1112, 2543
Eco88I CYCGRG 2 cut(s) 1394, 2527
Eco91I GGTNACC 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 2211
EcoNI CCTNNNNNAGG 1 cut(s) 2684
EcoO109I RGGNCCY 1 cut(s) 670
EcoO65I GGTNACC 1 cut(s) 317
EcoRI GAATTC 5 cut(s) 161, 882, 1024, 1131, 2503
EcoRII CCWGG 6 cut(s) 307, 616, 1102, 1267, 1891, 2685
EcoRV GATATC 1 cut(s) 2276
EcoT14I CCWWGG 6 cut(s) 59, 1185, 1277, 2831, 3082, 3152
EcoT22I ATGCAT 1 cut(s) 3451
EcoT38I GRGCYC 1 cut(s) 2213
ErhI CCWWGG 6 cut(s) 59, 1185, 1277, 2831, 3082, 3152
Esp3I CGTCTC 2 cut(s) 933, 1589
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FaqI GGGAC 2 cut(s) 788, 2343
FauNDI CATATG 1 cut(s) 343
FbaI TGATCA 2 cut(s) 1234, 3466
FokI GGATG 7 cut(s) 140, 473, 1997, 3458, 3494, 3593, 3662
FriOI GRGCYC 1 cut(s) 2213
FspBI CTAG 5 cut(s) 192, 771, 2234, 2793, 3153
GlaI GCGC 3 cut(s) 298, 1331, 1449
GsaI CCCAGC 1 cut(s) 2365
GsuI CTGGAG 4 cut(s) 330, 1086, 2306, 2669
HaeII RGCGCY 1 cut(s) 1333
HaeIII GGCC 8 cut(s) 145, 400, 672, 2888, 3042, 3081, 3093, 3321
HapII CCGG 5 cut(s) 1740, 2083, 2751, 3072, 3492
HgaI GACGC 4 cut(s) 1143, 1181, 1208, 1454
HhaI GCGC 3 cut(s) 299, 1332, 1450
Hin1I GRCGYC 1 cut(s) 1465
Hin6I GCGC 3 cut(s) 297, 1330, 1448
HinP1I GCGC 3 cut(s) 297, 1330, 1448
HincII GTYRAC 2 cut(s) 1198, 2667
HindII GTYRAC 2 cut(s) 1198, 2667
HindIII AAGCTT 3 cut(s) 171, 1607, 2954
HpaII CCGG 5 cut(s) 1740, 2083, 2751, 3072, 3492
Hpy166II GTNNAC 5 cut(s) 818, 1198, 2667, 3065, 3230
Hpy8I GTNNAC 5 cut(s) 818, 1198, 2667, 3065, 3230
Hpy99I CGWCG 1 cut(s) 1603
HpyCH4IV ACGT 8 cut(s) 820, 1402, 2521, 2785, 2898, 3226, 3281, 3378
HpySE526I ACGT 8 cut(s) 820, 1402, 2521, 2785, 2898, 3226, 3281, 3378
Hsp92I GRCGYC 1 cut(s) 1465
HspAI GCGC 3 cut(s) 297, 1330, 1448
KpnI GGTACC 2 cut(s) 2840, 3708
Ksp22I TGATCA 2 cut(s) 1234, 3466
LguI GCTCTTC 1 cut(s) 2610
LmnI GCTCC 8 cut(s) 281, 663, 829, 920, 1105, 1327, 3258, 3599
LweI GCATC 7 cut(s) 949, 1399, 1985, 2705, 2872, 3436, 3458
MaeI CTAG 5 cut(s) 192, 771, 2234, 2793, 3153
MaeII ACGT 8 cut(s) 820, 1402, 2521, 2785, 2898, 3226, 3281, 3378
MfeI CAATTG 2 cut(s) 2226, 3132
MflI RGATCY 8 cut(s) 227, 721, 970, 1905, 2281, 2966, 3000, 3710
MhlI GDGCHC 3 cut(s) 826, 2213, 3604
MlsI TGGCCA 3 cut(s) 145, 400, 3081
MluNI TGGCCA 3 cut(s) 145, 400, 3081
MlyI GAGTC 4 cut(s) 607, 770, 2186, 2914
MmeI TCCRAC 4 cut(s) 1913, 2184, 2682, 2984
Mox20I TGGCCA 3 cut(s) 145, 400, 3081
Mph1103I ATGCAT 1 cut(s) 3451
MroXI GAANNNNTTC 6 cut(s) 165, 882, 1131, 1135, 2604, 2679
MscI TGGCCA 3 cut(s) 145, 400, 3081
MslI CAYNNNNRTG 5 cut(s) 183, 394, 1490, 3474, 3480
Msp20I TGGCCA 3 cut(s) 145, 400, 3081
MspA1I CMGCKG 2 cut(s) 2672, 2852
MspI CCGG 5 cut(s) 1740, 2083, 2751, 3072, 3492
MspR9I CCNGG 8 cut(s) 309, 618, 1104, 1269, 1740, 1893, 2084, 2687
MunI CAATTG 2 cut(s) 2226, 3132
Mva1269I GAATGC 1 cut(s) 2074
MvaI CCWGG 6 cut(s) 309, 618, 1104, 1269, 1893, 2687
NciI CCSGG 2 cut(s) 1740, 2084
NcoI CCATGG 3 cut(s) 1277, 2831, 3082
NdeI CATATG 1 cut(s) 343
NlaIV GGNNCC 4 cut(s) 972, 2838, 3322, 3706
NmuCI GTSAC 7 cut(s) 317, 1049, 1120, 1787, 2917, 3551, 3731
NsiI ATGCAT 1 cut(s) 3451
NspI RCATGY 3 cut(s) 1484, 2474, 3479
NspV TTCGAA 1 cut(s) 1384
PaeR7I CTCGAG 2 cut(s) 1394, 2527
PagI TCATGA 4 cut(s) 178, 481, 1231, 2976
PciI ACATGT 2 cut(s) 1480, 2470
PciSI GCTCTTC 1 cut(s) 2610
PcsI WCGNNNNNNNCGW 2 cut(s) 162, 3238
PctI GAATGC 1 cut(s) 2074
PdmI GAANNNNTTC 6 cut(s) 165, 882, 1131, 1135, 2604, 2679
PflFI GACNNNGTC 1 cut(s) 3332
PflMI CCANNNNNTGG 2 cut(s) 2368, 2692
PfoI TCCNGGA 2 cut(s) 307, 1102
PinAI ACCGGT 1 cut(s) 2750
PleI GAGTC 4 cut(s) 607, 770, 2186, 2914
PmaCI CACGTG 1 cut(s) 3227
PmlI CACGTG 1 cut(s) 3227
PpsI GAGTC 4 cut(s) 607, 770, 2186, 2914
Ppu21I YACGTR 3 cut(s) 821, 2522, 3227
PscI ACATGT 2 cut(s) 1480, 2470
PshBI ATTAAT 1 cut(s) 1352
PsiI TTATAA 1 cut(s) 3342
Psp124BI GAGCTC 1 cut(s) 2213
Psp1406I AACGTT 2 cut(s) 1402, 3281
Psp6I CCWGG 6 cut(s) 307, 616, 1102, 1267, 1891, 2685
PspCI CACGTG 1 cut(s) 3227
PspEI GGTNACC 1 cut(s) 317
PspFI CCCAGC 1 cut(s) 2361
PspGI CCWGG 6 cut(s) 307, 616, 1102, 1267, 1891, 2685
PspN4I GGNNCC 4 cut(s) 972, 2838, 3322, 3706
PspPI GGNCC 5 cut(s) 440, 670, 1712, 2619, 3320
PspXI VCTCGAGB 1 cut(s) 2527
PstI CTGCAG 1 cut(s) 1207
PstNI CAGNNNCTG 5 cut(s) 1534, 2368, 2692, 2855, 2914
PsuI RGATCY 8 cut(s) 227, 721, 970, 1905, 2281, 2966, 3000, 3710
PsyI GACNNNGTC 1 cut(s) 3332
PvuII CAGCTG 2 cut(s) 2672, 2852
RseI CAYNNNNRTG 5 cut(s) 183, 394, 1490, 3474, 3480
SacI GAGCTC 1 cut(s) 2213
SapI GCTCTTC 1 cut(s) 2610
Sau96I GGNCC 5 cut(s) 440, 670, 1712, 2619, 3320
ScaI AGTACT 4 cut(s) 861, 1307, 1703, 3204
SchI GAGTC 4 cut(s) 607, 770, 2186, 2914
ScrFI CCNGG 8 cut(s) 309, 618, 1104, 1269, 1740, 1893, 2084, 2687
SduI GDGCHC 3 cut(s) 826, 2213, 3604
SfaNI GCATC 7 cut(s) 949, 1399, 1985, 2705, 2872, 3436, 3458
SfcI CTRYAG 4 cut(s) 829, 1203, 2903, 3727
Sfr274I CTCGAG 2 cut(s) 1394, 2527
SfuI TTCGAA 1 cut(s) 1384
SinI GGWCC 3 cut(s) 440, 1712, 2619
SlaI CTCGAG 2 cut(s) 1394, 2527
SmiMI CAYNNNNRTG 5 cut(s) 183, 394, 1490, 3474, 3480
SmlI CTYRAG 3 cut(s) 656, 1394, 2527
SmoI CTYRAG 3 cut(s) 656, 1394, 2527
SsiI CCGC 7 cut(s) 4, 315, 412, 443, 2885, 3392, 3408
SspI AATATT 5 cut(s) 408, 891, 2040, 2325, 2726
SspMI CTAG 5 cut(s) 192, 771, 2234, 2793, 3153
SstI GAGCTC 1 cut(s) 2213
StyD4I CCNGG 8 cut(s) 307, 616, 1102, 1267, 1738, 1891, 2082, 2685
StyI CCWWGG 6 cut(s) 59, 1185, 1277, 2831, 3082, 3152
TaiI ACGT 8 cut(s) 823, 1405, 2524, 2788, 2901, 3229, 3284, 3381
TaqII GACCGA 1 cut(s) 2636
TatI WGTACW 6 cut(s) 8, 859, 1305, 1701, 3190, 3202
TauI GCSGC 3 cut(s) 415, 2888, 3395
TseFI GTSAC 7 cut(s) 317, 1049, 1120, 1787, 2917, 3551, 3731
Tsp45I GTSAC 7 cut(s) 317, 1049, 1120, 1787, 2917, 3551, 3731
TspGWI ACGGA 3 cut(s) 1068, 1603, 2174
Tth111I GACNNNGTC 1 cut(s) 3332
Van91I CCANNNNNTGG 2 cut(s) 2368, 2692
VpaK11BI GGWCC 3 cut(s) 440, 1712, 2619
VspI ATTAAT 1 cut(s) 1352
XagI CCTNNNNNAGG 1 cut(s) 2684
XapI RAATTY 7 cut(s) 161, 882, 1024, 1131, 2394, 2503, 3461
XceI RCATGY 3 cut(s) 1484, 2474, 3479
XcmI CCANNNNNNNNNTGG 1 cut(s) 2438
XhoI CTCGAG 2 cut(s) 1394, 2527
XmaJI CCTAGG 1 cut(s) 3152
XmnI GAANNNNTTC 6 cut(s) 165, 882, 1131, 1135, 2604, 2679
XspI CTAG 5 cut(s) 192, 771, 2234, 2793, 3153
ZrmI AGTACT 4 cut(s) 861, 1307, 1703, 3204
Zsp2I ATGCAT 1 cut(s) 3451
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.