RchiOBHm_Chr2g0127091

HB1, ASXL, restriction endonuclease HTH domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0002907
Physical Location & Seq
Forward (+)
41857000 .. 41867596
10597 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ49899

Sequence Viewer

Length: 3954 bp
ATGAGACAAAAAGAAGAATTACGCAGAGAGAAAGAGGCAGCAAGGCTTAAAGCTGCTAATGAGAGAGCTATTGCTCGTAAAAATGCTAAAGAATCAATGGAACTCATTGAGGATGAATGCCTGGAACTCATGGAGTTAGCTGCATCAAGCAAGGAGCTTCCTTCCATGTTGTCTCTTGATTATGAAACCTTGCAAAATCTTGAATCATTTAGAGATATGCGGAGTACATTCCCACCCAAATCTGTGCAGTTGAAAAAACCTTTTGGTATTCAACCTTGGATAGATTCAGAGGAGAACATTGGCAATCTTCTTATGGCATGGAGATTCTTAATTACTTTTGTGGATGTTCTTGGGCTTTGGCCATTTACTCTCGATGAATTCGTTCAAGCTTTTCATGACTATGATTCTAGGTTGTTGGGTGAGATACATATTTCACTTCTAAGATCCATCATTAAAGACATTGAAGATGTTGCAAGAATACCCTCTATGGGGGTGGGAGCTAATCAGAACTGCGCTGCAAATCCTGGAGGCGGTCACCCTCAAATTGTAGAAGGGGCATATGCTTGGGGATTTGACATCAAAAGCTGGAAGTGCAACCTAAATCCACTGACATGGCCAGAAATATTGCGGCAATTTTCTGTATCAGCAGGATTTGGACCGCAACTGAAGAAGAGGGATGTTGAACCAGCTTATCTTCATGAGGACAATGAGGGTAATGATGTCAAGGATATAATTTCCAATTTACGCAGTGGAGTAGCTGTAAAAAATGCCTTCGCTATTATGCAAGAAAGGGGTTTTTCTAACCCTCGCAAATCAAGGCATTGCTTGACTCCTGGCACTGTTAAGTTCGCAGCATTTCATGTTCTTTCTCTTGAGGGGAGCAAGGGCCTTAACATATTAGAAGTTGCAGACAGGATTCAGAAATCAGGACTTCGGGATCTCACAACAAGCAAGACACCAGAAGCCTCGATAGCTGCTGCTTTGTCTAGGGACTCAAAACTATTTGAGAGAACTGCACCTTCTACTTACTGTGTACGTGCTCCCTACAGAAAGGACACAAGTGATTCCGAGGCAGTACTTTCGGCAGCCAGAGAAAGAATTCAAATATTCAATAGTAGGATTTTTGATGTTGAAGGAGCTGATGAAGCTGAAAGAGACGAAGAATCTGAAAGTGATGCAGTAGAGGATCCTGAGTTTGATGATTTAGGCACTGAAATAAATCCAAAGATAGTAGCTCAGAATTCTGAAGAAGCAAGAAAAGTCAGTGACGGAATATCTTTGGAAAGTGGAAAAGGAAGTTACGAGGTTAGTAAAGCTCCAGGAGACCTTAGGAATGTCACAGAAGGAATTCCTTCAATCAATTCTGAAGCGTCCATCAAGGTTGAAGACGCTGGTTGTTCCTTGGATTCTGTTGACGCTGCAGGAATCTGCAACAATGTAGCTAATCATGATCAAGAAGATACAGAGATTGATGATAGCAACCCTGGTGAGCCATGGGTTCAAGGACTTACAGAAGGGGAGTACTCTGATCTTAGTGTTGAGGAGCGCCTCAATGCACTTGCTGCATTAATTGGTGTGGCAATAGAAGGAAACTCGATTCGAATTGTTCTCGAGGAACGTTTAGATGCTGCAAATGCTTTAAAGAAGCAAATGTGGGCAGTGGCGCAAGTTGATAAACGGCGTCTAAAAGAAGAACATGTCATCAAAATGCAATATACCCCTTTCATTGGGAACAAGACTGAGCAGAGCCTGACAATTCCTTCAGAAAATAGGCAAAGTCCATCTCCTACCGTTGATGAGAAAAACATTGAGACGACGGTGAAGCTTTCCGTCCTGCAAGAGCAGATGGTTGATCCACAAAATGAGCAAAATTATATCAACAGTTTTCCTTCTGTACGAAACCTCCAAATGCAGGAGTACTCTGTTGGTCCAGACAATCACCCATTTCAGCAACCCGGTCTTATTGCTGATAAGTCGCACAAACAATTAAAATCTTATATTGGTCACAAAGCAGAGGAGATGTATGTTTATCGATCATTGCCTCTTGGTCAGGATCGTAGGCATAATAGATACTGGCAGTTTATTACATCTGCCTCTCGAAATGATCCTGGTTGTGGCAGGATCTTTGTTGAGCTGCATGACGGTCGTTGGAGGCTTATTGATTCTGAAGAGGGCTTTGATGGTCTTTTGGCATCTTTGGATGTGCGTGGGAATAGGGAATCCCATTTGCAGACAATGTTGCAGAAGACTGAAATATTCTTCAAGGAAACTGTTAGAAGAAACAAGTTGCATTCTAACCGGGGGAGGCATATCAAAGACACTGCTAAAACAGAAGATGTTGAAATGCCTTCTGGTTATGATTGCAGTGGTGGTACGGATAGCCCTACCAGCTCTGTTTCTGTTGCTGACTCCGATATGTTGGAGAGCTCAATGACTTTTTCAATTGAGCTAGGAAAGAACGAAACTGAGAAAACTGGTGCTTTAAACAGATATCAAGATCTGGAGAGGTGGATTTTGAAAGAAAGTGTTGGTTCTTCAATATTATGTGCTGACAAAAATGGGGAAAAAAGGTGTCCCCAGCTACTGGACATCTGCAATTCTTGTCGTGGAATTTTTTATTTTGAAGAGAATCACTGTCATTCTTGCCATAGAACTTTTGGCAAGGGCGAAATAGATTTTTCACAACATGTGGTACTGTGTAAAGAGAAACTGAAGTTGAATTCTGATTGTAATTTACGTGGCTCGAGTTCTTCTCCCCTTAGGATCAGGTTGCTGAAGGTGCTATTAGCTTTGACTGAGGTTTCTGTTCCATTAGAAGCTCTTCAACCTCTTTGGACCGACATGAACCGTAAATCTTGGGGTAGAAAGTTACATTCCTCGTCAACAGCTGAAGACCTTCTCCAGGTTCTGGCATCGTTGGAGAGTGCTGTAAAGATAGAATATTTATCTTCAAACTTTGAGACTACCGGTGAGCTGCTATATTCCTCAAATGAAAAAGGACATGTTACTAGCAATTCTTCTTCTCCTGATAGCGTTGCTGTGCTTCCATGGGTACCACTTACAACAGCTGCTGTGGCTCTTAGGCTCATGGAGTTTGATGCGGCCATCTCAAACATGCTACAGCAGAAGCTGATGATGACTCAGAAGGACAAGGAATCTGGGGATTTTAAACTTCCAACAAGATCTGCTGTCATGAAAAGTTCCAAGAATGAGGAGATCTCGGGAACTCCACATCAATCTGAAGATTCTAAAGAAGGCCACTGGGAGTATCTGGGGAGTGGACTTACCGGCTCTGGCCATGGACGAGGCCGTGGTCGAACTGGTGGTAGGCGAGTGCCAAAAAAGGCAATTGGTTTAAGGGGCAATCCTAGGAAGAAGAGTAGAGTTACAGCCAAAGATAAATTAGTACAGGTGCAAGTACTGAAATGGAAAGGCAGACCACGTGGACGAGGAGGGCGAAAGCGTGGAAATCGGAGCATTAGAGGCAAGGAGACAACGTTTAACAGGATAATTAAGATCGGTGGGGAGAGAACTGGCCCCAAAGACAGTGTCTATTATAAATCACCAGATGACTTGGTTGAGGAGAATTGGAACGTAGGTGAAACTGCGGCAGAAAATGCAAGCGGTTCTGGAAGGTCAGAGTATGAAGTGGAAAATGGGGATGCATCAGGAGATGAATTTGATCATGTGCATGTGGATGACTATGCCGGTGGCTTCAATGGCAAGTCTGATGACTTATTGGAGGGAGGCGAATATAACATGGAGGGTGATGAAGATGATGTCGACAATGAGGTGGATGACGATGAAGGTGAAGGAGCACAGGATTCGGATTTTGATGGTGCAAGATATATCAATGGAGAGTGGGATGAAGAGGAACACATAGCTGATGATGGACAGCAAGACATGGAACTTGGTGAAGGTACCAGATCTTCGTCTTCAGACTATAGTGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000976 GO:0000977 GO:0000978 GO:0000981 GO:0000982 GO:0000987 GO:0001012 GO:0001067 GO:0001077 GO:0001228 GO:0001525 GO:0001568 GO:0001944 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009791 GO:0009889 GO:0009890 GO:0009891 GO:0009892 GO:0009893 GO:0009987 GO:0010228 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010558 GO:0010604 GO:0010605 GO:0010628 GO:0010629 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0022414 GO:0031323 GO:0031324 GO:0031325 GO:0031326 GO:0031327 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0034641 GO:0034645 GO:0034654 GO:0035239 GO:0035295 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044464 GO:0045892 GO:0045893 GO:0045934 GO:0045935 GO:0045944 GO:0046483 GO:0048514 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048608 GO:0048646 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051171 GO:0051172 GO:0051173 GO:0051252 GO:0051253 GO:0051254 GO:0060255 GO:0061458 GO:0065007 GO:0071704 GO:0072358 GO:0072359 GO:0080090 GO:0090304 GO:0097159 GO:0097659 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902679 GO:1902680 GO:1903506 GO:1903507 GO:1903508 GO:1990837 GO:2000112 GO:2000113 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DDT HARE-HTH Homeobox WHIM1 WSD

Protein Analysis

1317

Amino Acids

146.72

Weight (kDa)

5.09

Isoelectric Point (pI)

46.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DDT PF02791 98 - 153 9.3e-19 DDT domain
HARE-HTH PF05066 279 - 346 8.7e-18 HB1, ASXL, restriction endonuclease HTH domain
WHIM1 PF15612 495 - 538 2.9e-10 WSTF, HB1, Itc1p, MBD9 motif 1
WSD PF15613 677 - 750 5.6e-22 Williams-Beuren syndrome DDT (WSD), D-TOX E motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 3558
Acc65I GGTACC 2 cut(s) 3052, 3920
AccB1I GGYRCC 2 cut(s) 3052, 3920
AccB7I CCANNNNNTGG 2 cut(s) 2584, 2908
AccI GTMKAC 1 cut(s) 3783
AciI CCGC 7 cut(s) 220, 531, 628, 659, 3101, 3608, 3624
AclI AACGTT 2 cut(s) 1618, 3497
AclWI GGATC 9 cut(s) 438, 945, 1181, 1194, 1847, 2061, 2099, 2129, 2771
AcoI YGGCCR 4 cut(s) 359, 614, 3102, 3295
AcsI RAATTY 7 cut(s) 377, 1098, 1240, 1347, 2610, 2719, 3677
AcvI CACGTG 1 cut(s) 3443
AcyI GRCGYC 1 cut(s) 1681
AfiI CCNNNNNNNGG 9 cut(s) 488, 489, 530, 1727, 1912, 2114, 2584, 2902, 2908
AflIII ACRYGT 3 cut(s) 1696, 2686, 3001
AgeI ACCGGT 1 cut(s) 2966
AjnI CCWGG 7 cut(s) 120, 523, 832, 1318, 1483, 2107, 2901
Alw21I GWGCWC 3 cut(s) 1042, 2429, 3820
Alw26I GTCTC 6 cut(s) 177, 1149, 1317, 1805, 2954, 3485
AlwI GGATC 9 cut(s) 438, 945, 1181, 1194, 1847, 2061, 2099, 2129, 2771
AlwNI CAGNNNCTG 5 cut(s) 1750, 2584, 2908, 3071, 3130
Ama87I CYCGRG 3 cut(s) 1610, 2743, 3220
AoxI GGCC 8 cut(s) 359, 614, 886, 3102, 3256, 3295, 3307, 3535
ApoI RAATTY 7 cut(s) 377, 1098, 1240, 1347, 2610, 2719, 3677
ArsI GACNNNNNNTTYG 2 cut(s) 3534, 3566
AseI ATTAAT 1 cut(s) 1568
AsiGI ACCGGT 1 cut(s) 2966
Asp700I GAANNNNTTC 6 cut(s) 381, 1098, 1347, 1351, 2820, 2895
Asp718I GGTACC 2 cut(s) 3052, 3920
AspA2I CCTAGG 1 cut(s) 3368
AspLEI GCGC 3 cut(s) 515, 1548, 1666
AspS9I GGNCC 5 cut(s) 656, 886, 1928, 2835, 3536
AsuC2I CCSGG 2 cut(s) 1956, 2300
AsuII TTCGAA 1 cut(s) 1600
AvaI CYCGRG 3 cut(s) 1610, 2743, 3220
AvaII GGWCC 3 cut(s) 656, 1928, 2835
AvrII CCTAGG 1 cut(s) 3368
AxyI CCTNAGG 2 cut(s) 1328, 2759
BalI TGGCCA 3 cut(s) 361, 616, 3297
BamHI GGATCC 1 cut(s) 1186
BanI GGYRCC 2 cut(s) 3052, 3920
BanII GRGCYC 1 cut(s) 2429
BarI GAAGNNNNNNTAC 2 cut(s) 3913, 3945
BbrPI CACGTG 1 cut(s) 3443
BbsI GAAGAC 4 cut(s) 1392, 2252, 2898, 3927
Bbv12I GWGCWC 3 cut(s) 1042, 2429, 3820
BccI CCATC 8 cut(s) 455, 1382, 1789, 1840, 2174, 3113, 3830, 3884
BceAI ACGGC 2 cut(s) 1694, 3294
BcgI CGANNNNNNTGC 6 cut(s) 1062, 1096, 2126, 2160, 3807, 3841
BciT130I CCWGG 7 cut(s) 122, 525, 834, 1320, 1485, 2109, 2903
BclI TGATCA 2 cut(s) 1450, 3682
BcnI CCSGG 2 cut(s) 1956, 2300
BcoDI GTCTC 6 cut(s) 177, 1149, 1317, 1805, 2954, 3485
BfaI CTAG 5 cut(s) 408, 987, 2450, 3009, 3369
BfmI CTRYAG 4 cut(s) 1045, 1419, 3119, 3943
BfoI RGCGCY 1 cut(s) 1549
BglII AGATCT 4 cut(s) 2497, 3182, 3216, 3926
BlnI CCTAGG 1 cut(s) 3368
BmcAI AGTACT 4 cut(s) 1077, 1523, 1919, 3420
Bme1390I CCNGG 9 cut(s) 122, 525, 834, 1320, 1485, 1956, 2109, 2300, 2903
Bme18I GGWCC 3 cut(s) 656, 1928, 2835
BmeT110I CYCGRG 3 cut(s) 1610, 2743, 3220
BmgT120I GGNCC 5 cut(s) 656, 886, 1928, 2835, 3536
BmiI GGNNCC 4 cut(s) 1188, 3054, 3538, 3922
BmrFI CCNGG 9 cut(s) 122, 525, 834, 1320, 1485, 1956, 2109, 2300, 2903
BmrI ACTGGG 1 cut(s) 3271
BmsI GCATC 8 cut(s) 152, 1165, 1615, 2201, 2921, 3088, 3652, 3674
BmuI ACTGGG 1 cut(s) 3271
BpiI GAAGAC 4 cut(s) 1392, 2252, 2898, 3927
BplI GAGNNNNNCTC 4 cut(s) 2737, 2769, 3203, 3235
BpmI CTGGAG 4 cut(s) 546, 1302, 2522, 2885
Bpu14I TTCGAA 1 cut(s) 1600
BpuEI CTTGAG 1 cut(s) 893
BpuMI CCSGG 2 cut(s) 1956, 2300
Bsa29I ATCGAT 1 cut(s) 2032
BsaAI YACGTR 3 cut(s) 1037, 2738, 3443
BsaHI GRCGYC 1 cut(s) 1681
BsaI GGTCTC 1 cut(s) 1317
BsaWI WCCGGW 1 cut(s) 2966
BsaXI ACNNNNNCTCC 6 cut(s) 744, 774, 1887, 1917, 3482, 3512
Bsc4I CCNNNNNNNGG 9 cut(s) 488, 489, 530, 1727, 1912, 2114, 2584, 2902, 2908
Bse118I RCCGGY 3 cut(s) 2966, 3287, 3707
Bse1I ACTGG 6 cut(s) 2078, 2479, 2589, 3266, 3325, 3538
Bse21I CCTNAGG 2 cut(s) 1328, 2759
Bse3DI GCAATG 2 cut(s) 820, 2036
BseBI CCWGG 7 cut(s) 122, 525, 834, 1320, 1485, 2109, 2903
BseCI ATCGAT 1 cut(s) 2032
BseGI GGATG 8 cut(s) 118, 349, 682, 2206, 3667, 3703, 3802, 3871
BseLI CCNNNNNNNGG 9 cut(s) 488, 489, 530, 1727, 1912, 2114, 2584, 2902, 2908
BseMI GCAATG 2 cut(s) 820, 2036
BseMII CTCAG 6 cut(s) 1182, 1250, 1731, 2457, 2787, 3155
BseNI ACTGG 6 cut(s) 2078, 2479, 2589, 3266, 3325, 3538
BseRI GAGGAG 6 cut(s) 305, 1556, 2030, 3227, 3465, 3596
BseYI CCCAGC 1 cut(s) 2577
BsgI GTGCAG 2 cut(s) 266, 999
Bsh1285I CGRYCG 1 cut(s) 2146
BshFI GGCC 8 cut(s) 361, 616, 888, 3104, 3258, 3297, 3309, 3537
BshNI GGYRCC 2 cut(s) 3052, 3920
BshTI ACCGGT 1 cut(s) 2966
BshVI ATCGAT 1 cut(s) 2032
BsiEI CGRYCG 1 cut(s) 2146
BsiHKAI GWGCWC 3 cut(s) 1042, 2429, 3820
BsiHKCI CYCGRG 3 cut(s) 1610, 2743, 3220
BsiSI CCGG 5 cut(s) 1956, 2299, 2967, 3288, 3708
BslFI GGGAC 2 cut(s) 1004, 2559
BslI CCNNNNNNNGG 9 cut(s) 488, 489, 530, 1727, 1912, 2114, 2584, 2902, 2908
BsmAI GTCTC 6 cut(s) 177, 1149, 1317, 1805, 2954, 3485
BsmBI CGTCTC 2 cut(s) 1149, 1805
BsmFI GGGAC 2 cut(s) 1004, 2559
BsmI GAATGC 2 cut(s) 122, 2290
BsnI GGCC 8 cut(s) 361, 616, 888, 3104, 3258, 3297, 3309, 3537
Bso31I GGTCTC 1 cut(s) 1317
BsoBI CYCGRG 3 cut(s) 1610, 2743, 3220
Bsp119I TTCGAA 1 cut(s) 1600
Bsp1286I GDGCHC 3 cut(s) 1042, 2429, 3820
Bsp19I CCATGG 3 cut(s) 1493, 3047, 3298
BspACI CCGC 7 cut(s) 220, 531, 628, 659, 3101, 3608, 3624
BspANI GGCC 8 cut(s) 361, 616, 888, 3104, 3258, 3297, 3309, 3537
BspCNI CTCAG 6 cut(s) 1183, 1249, 1732, 2458, 2788, 3154
BspDI ATCGAT 1 cut(s) 2032
BspHI TCATGA 4 cut(s) 394, 697, 1447, 3192
BspLI GGNNCC 4 cut(s) 1188, 3054, 3538, 3922
BspMAI CTGCAG 1 cut(s) 1423
BspPI GGATC 9 cut(s) 438, 945, 1181, 1194, 1847, 2061, 2099, 2129, 2771
BspQI GCTCTTC 1 cut(s) 2826
BspT104I TTCGAA 1 cut(s) 1600
BspT107I GGYRCC 2 cut(s) 3052, 3920
BspTNI GGTCTC 1 cut(s) 1317
BsrDI GCAATG 2 cut(s) 820, 2036
BsrFI RCCGGY 3 cut(s) 2966, 3287, 3707
BsrI ACTGG 6 cut(s) 2078, 2479, 2589, 3266, 3325, 3538
BssAI RCCGGY 3 cut(s) 2966, 3287, 3707
BssNI GRCGYC 1 cut(s) 1681
BssT1I CCWWGG 6 cut(s) 275, 1401, 1493, 3047, 3298, 3368
Bst2UI CCWGG 7 cut(s) 122, 525, 834, 1320, 1485, 2109, 2903
Bst6I CTCTTC 6 cut(s) 665, 2163, 2619, 2826, 3371, 3864
BstACI GRCGYC 1 cut(s) 1681
BstAPI GCANNNNNTGC 2 cut(s) 1562, 3617
BstBAI YACGTR 3 cut(s) 1037, 2738, 3443
BstBI TTCGAA 1 cut(s) 1600
BstC8I GCNNGC 1 cut(s) 3622
BstDSI CCRYGG 4 cut(s) 1493, 3047, 3298, 3310
BstEII GGTNACC 1 cut(s) 533
BstENI CCTNNNNNAGG 1 cut(s) 2900
BstF5I GGATG 8 cut(s) 118, 349, 682, 2206, 3667, 3703, 3802, 3871
BstH2I RGCGCY 1 cut(s) 1549
BstHHI GCGC 3 cut(s) 515, 1548, 1666
BstMAI GTCTC 6 cut(s) 177, 1149, 1317, 1805, 2954, 3485
BstMCI CGRYCG 1 cut(s) 2146
BstNI CCWGG 7 cut(s) 122, 525, 834, 1320, 1485, 2109, 2903
BstNSI RCATGY 5 cut(s) 1700, 2690, 3005, 3118, 3695
BstPI GGTNACC 1 cut(s) 533
BstSCI CCNGG 9 cut(s) 120, 523, 832, 1318, 1483, 1954, 2107, 2298, 2901
BstSFI CTRYAG 4 cut(s) 1045, 1419, 3119, 3943
BstV2I GAAGAC 4 cut(s) 1392, 2252, 2898, 3927
BstX2I RGATCY 8 cut(s) 443, 937, 1186, 2121, 2497, 3182, 3216, 3926
BstXI CCANNNNNNTGG 1 cut(s) 612
BstYI RGATCY 8 cut(s) 443, 937, 1186, 2121, 2497, 3182, 3216, 3926
Bsu15I ATCGAT 1 cut(s) 2032
Bsu36I CCTNAGG 2 cut(s) 1328, 2759
BsuRI GGCC 8 cut(s) 361, 616, 888, 3104, 3258, 3297, 3309, 3537
BsuTUI ATCGAT 1 cut(s) 2032
BtgI CCRYGG 4 cut(s) 1493, 3047, 3298, 3310
BtsCI GGATG 8 cut(s) 118, 349, 682, 2206, 3667, 3703, 3802, 3871
BtsI GCAGTG 4 cut(s) 754, 1665, 2319, 2371
Cac8I GCNNGC 1 cut(s) 3622
CaiI CAGNNNCTG 5 cut(s) 1750, 2584, 2908, 3071, 3130
CciI TCATGA 4 cut(s) 394, 697, 1447, 3192
CfoI GCGC 3 cut(s) 515, 1548, 1666
Cfr10I RCCGGY 3 cut(s) 2966, 3287, 3707
Cfr13I GGNCC 5 cut(s) 656, 886, 1928, 2835, 3536
ClaI ATCGAT 1 cut(s) 2032
CseI GACGC 4 cut(s) 1359, 1397, 1424, 1670
CspAI ACCGGT 1 cut(s) 2966
DraI TTTAAA 3 cut(s) 1641, 2484, 3169
EaeI YGGCCR 4 cut(s) 359, 614, 3102, 3295
Eam1104I CTCTTC 6 cut(s) 665, 2163, 2619, 2826, 3371, 3864
EarI CTCTTC 6 cut(s) 665, 2163, 2619, 2826, 3371, 3864
Ecl136II GAGCTC 1 cut(s) 2427
Eco130I CCWWGG 6 cut(s) 275, 1401, 1493, 3047, 3298, 3368
Eco24I GRGCYC 1 cut(s) 2429
Eco31I GGTCTC 1 cut(s) 1317
Eco32I GATATC 1 cut(s) 2492
Eco47I GGWCC 3 cut(s) 656, 1928, 2835
Eco53kI GAGCTC 1 cut(s) 2427
Eco72I CACGTG 1 cut(s) 3443
Eco81I CCTNAGG 2 cut(s) 1328, 2759
Eco88I CYCGRG 3 cut(s) 1610, 2743, 3220
Eco91I GGTNACC 1 cut(s) 533
EcoICRI GAGCTC 1 cut(s) 2427
EcoNI CCTNNNNNAGG 1 cut(s) 2900
EcoO109I RGGNCCY 1 cut(s) 886
EcoO65I GGTNACC 1 cut(s) 533
EcoRI GAATTC 5 cut(s) 377, 1098, 1240, 1347, 2719
EcoRII CCWGG 7 cut(s) 120, 523, 832, 1318, 1483, 2107, 2901
EcoRV GATATC 1 cut(s) 2492
EcoT14I CCWWGG 6 cut(s) 275, 1401, 1493, 3047, 3298, 3368
EcoT22I ATGCAT 1 cut(s) 3667
EcoT38I GRGCYC 1 cut(s) 2429
ErhI CCWWGG 6 cut(s) 275, 1401, 1493, 3047, 3298, 3368
Esp3I CGTCTC 2 cut(s) 1149, 1805
FaqI GGGAC 2 cut(s) 1004, 2559
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 2 cut(s) 1450, 3682
FblI GTMKAC 1 cut(s) 3783
FokI GGATG 8 cut(s) 125, 356, 689, 2213, 3674, 3710, 3809, 3878
FriOI GRGCYC 1 cut(s) 2429
FspBI CTAG 5 cut(s) 408, 987, 2450, 3009, 3369
GlaI GCGC 3 cut(s) 514, 1547, 1665
GsaI CCCAGC 1 cut(s) 2581
GsuI CTGGAG 4 cut(s) 546, 1302, 2522, 2885
HaeII RGCGCY 1 cut(s) 1549
HaeIII GGCC 8 cut(s) 361, 616, 888, 3104, 3258, 3297, 3309, 3537
HapII CCGG 5 cut(s) 1956, 2299, 2967, 3288, 3708
HgaI GACGC 4 cut(s) 1359, 1397, 1424, 1670
HhaI GCGC 3 cut(s) 515, 1548, 1666
Hin1I GRCGYC 1 cut(s) 1681
Hin6I GCGC 3 cut(s) 513, 1546, 1664
HinP1I GCGC 3 cut(s) 513, 1546, 1664
HincII GTYRAC 3 cut(s) 1414, 2883, 3784
HindII GTYRAC 3 cut(s) 1414, 2883, 3784
HindIII AAGCTT 2 cut(s) 387, 1823
HpaII CCGG 5 cut(s) 1956, 2299, 2967, 3288, 3708
Hpy166II GTNNAC 6 cut(s) 1034, 1414, 2883, 3281, 3446, 3784
Hpy8I GTNNAC 6 cut(s) 1034, 1414, 2883, 3281, 3446, 3784
Hpy99I CGWCG 1 cut(s) 1819
HpyCH4IV ACGT 6 cut(s) 1036, 1618, 2737, 3442, 3497, 3594
HpySE526I ACGT 6 cut(s) 1036, 1618, 2737, 3442, 3497, 3594
Hsp92I GRCGYC 1 cut(s) 1681
HspAI GCGC 3 cut(s) 513, 1546, 1664
KpnI GGTACC 2 cut(s) 3056, 3924
Ksp22I TGATCA 2 cut(s) 1450, 3682
LguI GCTCTTC 1 cut(s) 2826
LmnI GCTCC 9 cut(s) 154, 497, 879, 1045, 1136, 1321, 1543, 3474, 3815
LweI GCATC 8 cut(s) 152, 1165, 1615, 2201, 2921, 3088, 3652, 3674
MaeI CTAG 5 cut(s) 408, 987, 2450, 3009, 3369
MaeII ACGT 6 cut(s) 1036, 1618, 2737, 3442, 3497, 3594
MaeIII GTNAC 9 cut(s) 533, 1265, 1298, 1336, 2003, 2868, 3004, 3385, 3947
MfeI CAATTG 2 cut(s) 2442, 3348
MflI RGATCY 8 cut(s) 443, 937, 1186, 2121, 2497, 3182, 3216, 3926
MhlI GDGCHC 3 cut(s) 1042, 2429, 3820
MlsI TGGCCA 3 cut(s) 361, 616, 3297
MluNI TGGCCA 3 cut(s) 361, 616, 3297
MlyI GAGTC 4 cut(s) 823, 986, 2402, 3133
MmeI TCCRAC 4 cut(s) 2129, 2400, 2898, 3200
Mox20I TGGCCA 3 cut(s) 361, 616, 3297
Mph1103I ATGCAT 1 cut(s) 3667
MroXI GAANNNNTTC 6 cut(s) 381, 1098, 1347, 1351, 2820, 2895
MscI TGGCCA 3 cut(s) 361, 616, 3297
MslI CAYNNNNRTG 5 cut(s) 399, 610, 1706, 3690, 3696
Msp20I TGGCCA 3 cut(s) 361, 616, 3297
MspA1I CMGCKG 2 cut(s) 2888, 3068
MspI CCGG 5 cut(s) 1956, 2299, 2967, 3288, 3708
MspR9I CCNGG 9 cut(s) 122, 525, 834, 1320, 1485, 1956, 2109, 2300, 2903
MunI CAATTG 2 cut(s) 2442, 3348
Mva1269I GAATGC 2 cut(s) 122, 2290
MvaI CCWGG 7 cut(s) 122, 525, 834, 1320, 1485, 2109, 2903
NciI CCSGG 2 cut(s) 1956, 2300
NcoI CCATGG 3 cut(s) 1493, 3047, 3298
NdeI CATATG 1 cut(s) 559
NlaIV GGNNCC 4 cut(s) 1188, 3054, 3538, 3922
NmuCI GTSAC 5 cut(s) 533, 1265, 1336, 2003, 3947
NsiI ATGCAT 1 cut(s) 3667
NspI RCATGY 5 cut(s) 1700, 2690, 3005, 3118, 3695
NspV TTCGAA 1 cut(s) 1600
PaeR7I CTCGAG 2 cut(s) 1610, 2743
PagI TCATGA 4 cut(s) 394, 697, 1447, 3192
PciI ACATGT 3 cut(s) 1696, 2686, 3001
PciSI GCTCTTC 1 cut(s) 2826
PcsI WCGNNNNNNNCGW 2 cut(s) 378, 3454
PctI GAATGC 2 cut(s) 122, 2290
PdmI GAANNNNTTC 6 cut(s) 381, 1098, 1347, 1351, 2820, 2895
PflFI GACNNNGTC 1 cut(s) 3548
PflMI CCANNNNNTGG 2 cut(s) 2584, 2908
PfoI TCCNGGA 2 cut(s) 523, 1318
PinAI ACCGGT 1 cut(s) 2966
PleI GAGTC 4 cut(s) 823, 986, 2402, 3133
PmaCI CACGTG 1 cut(s) 3443
PmlI CACGTG 1 cut(s) 3443
PpsI GAGTC 4 cut(s) 823, 986, 2402, 3133
Ppu21I YACGTR 3 cut(s) 1037, 2738, 3443
PscI ACATGT 3 cut(s) 1696, 2686, 3001
PshBI ATTAAT 1 cut(s) 1568
PsiI TTATAA 1 cut(s) 3558
Psp124BI GAGCTC 1 cut(s) 2429
Psp1406I AACGTT 2 cut(s) 1618, 3497
Psp6I CCWGG 7 cut(s) 120, 523, 832, 1318, 1483, 2107, 2901
PspCI CACGTG 1 cut(s) 3443
PspEI GGTNACC 1 cut(s) 533
PspFI CCCAGC 1 cut(s) 2577
PspGI CCWGG 7 cut(s) 120, 523, 832, 1318, 1483, 2107, 2901
PspN4I GGNNCC 4 cut(s) 1188, 3054, 3538, 3922
PspPI GGNCC 5 cut(s) 656, 886, 1928, 2835, 3536
PspXI VCTCGAGB 1 cut(s) 2743
PstI CTGCAG 1 cut(s) 1423
PstNI CAGNNNCTG 5 cut(s) 1750, 2584, 2908, 3071, 3130
PsuI RGATCY 8 cut(s) 443, 937, 1186, 2121, 2497, 3182, 3216, 3926
PsyI GACNNNGTC 1 cut(s) 3548
PvuII CAGCTG 2 cut(s) 2888, 3068
RseI CAYNNNNRTG 5 cut(s) 399, 610, 1706, 3690, 3696
SacI GAGCTC 1 cut(s) 2429
SalI GTCGAC 1 cut(s) 3782
SapI GCTCTTC 1 cut(s) 2826
Sau96I GGNCC 5 cut(s) 656, 886, 1928, 2835, 3536
ScaI AGTACT 4 cut(s) 1077, 1523, 1919, 3420
SchI GAGTC 4 cut(s) 823, 986, 2402, 3133
ScrFI CCNGG 9 cut(s) 122, 525, 834, 1320, 1485, 1956, 2109, 2300, 2903
SduI GDGCHC 3 cut(s) 1042, 2429, 3820
SfaNI GCATC 8 cut(s) 152, 1165, 1615, 2201, 2921, 3088, 3652, 3674
SfcI CTRYAG 4 cut(s) 1045, 1419, 3119, 3943
Sfr274I CTCGAG 2 cut(s) 1610, 2743
SfuI TTCGAA 1 cut(s) 1600
SinI GGWCC 3 cut(s) 656, 1928, 2835
SlaI CTCGAG 2 cut(s) 1610, 2743
SmiMI CAYNNNNRTG 5 cut(s) 399, 610, 1706, 3690, 3696
SmlI CTYRAG 3 cut(s) 872, 1610, 2743
SmoI CTYRAG 3 cut(s) 872, 1610, 2743
SsiI CCGC 7 cut(s) 220, 531, 628, 659, 3101, 3608, 3624
SspI AATATT 5 cut(s) 624, 1107, 2256, 2541, 2942
SspMI CTAG 5 cut(s) 408, 987, 2450, 3009, 3369
SstI GAGCTC 1 cut(s) 2429
StyD4I CCNGG 9 cut(s) 120, 523, 832, 1318, 1483, 1954, 2107, 2298, 2901
StyI CCWWGG 6 cut(s) 275, 1401, 1493, 3047, 3298, 3368
TaiI ACGT 6 cut(s) 1039, 1621, 2740, 3445, 3500, 3597
TaqII GACCGA 1 cut(s) 2852
TatI WGTACW 6 cut(s) 224, 1075, 1521, 1917, 3406, 3418
TauI GCSGC 3 cut(s) 631, 3104, 3611
TseFI GTSAC 5 cut(s) 533, 1265, 1336, 2003, 3947
Tsp45I GTSAC 5 cut(s) 533, 1265, 1336, 2003, 3947
TspGWI ACGGA 3 cut(s) 1284, 1819, 2390
Tth111I GACNNNGTC 1 cut(s) 3548
Van91I CCANNNNNTGG 2 cut(s) 2584, 2908
VpaK11BI GGWCC 3 cut(s) 656, 1928, 2835
VspI ATTAAT 1 cut(s) 1568
XagI CCTNNNNNAGG 1 cut(s) 2900
XapI RAATTY 7 cut(s) 377, 1098, 1240, 1347, 2610, 2719, 3677
XceI RCATGY 5 cut(s) 1700, 2690, 3005, 3118, 3695
XcmI CCANNNNNNNNNTGG 1 cut(s) 2654
XhoI CTCGAG 2 cut(s) 1610, 2743
XmaJI CCTAGG 1 cut(s) 3368
XmiI GTMKAC 1 cut(s) 3783
XmnI GAANNNNTTC 6 cut(s) 381, 1098, 1347, 1351, 2820, 2895
XspI CTAG 5 cut(s) 408, 987, 2450, 3009, 3369
ZrmI AGTACT 4 cut(s) 1077, 1523, 1919, 3420
Zsp2I ATGCAT 1 cut(s) 3667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.