AT5G50740

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0002139
Physical Location & Seq
Reverse (-)
20635826 .. 20638104
2279 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G50740.5

Sequence Viewer

Length: 852 bp
ATGGAGGAGAAGAAATCTGAAGAGCCACAAGCGAAATCAGAAGATAAGAAACCAGAAGAAGAGAAAAAGAAAGAACCTCAAGAGATTGTGCTTAAGATTTTTATGCATTGTGAAGGTTGTGCTAAAAAAATCCATAGATGTCTTAAAGGCTTTGAAGGAGTGGAGGATGTAACTACTGATTGTAAGACTAGTAAAGTTGTGGTGAAAGGAGAGAAGGCAGATCCATTAAAAGTGTTGCAAAGATTGCAGAGGAAGAGCCATCGTCAAGTGGAGCTTATCTCACCGATTCCGGAGCCTAAACCGGTTTCTGATGAACCGGAGAAAAAGGAGAAGGAGAAACCTAAACCTGAGGAGAAAAAAGAAGAGGTAGTGACAGTGGTGCTGAGAGTTCACATGCATTGTGAAGCATGTGCCATGGAGATTCAAAAGAGGATCATGAGAATGAAAGGAGTGGAGTCTGTGGAACCAGATTTTAAAGCATCACAAGTGAGCGTGAAAGGAGTTTTTACGCCGGAAAAGCTAGTTGAGTTCATTTACAAGAGAATCGGGAAGCATGCCGCGGTTGTCAAGCAAGATCCGCCGCCAAAACCTCCGGAGAAAGAAAAAGAAACGAAAGACAAAGACGAGAAGAAAAAGGAAGAGGGACAGCCCAAAGAAGGAAAAGAGGCCAAGGAAAACGGTGGTGGAGGAGGTGCTAAAGGCGATGGTGCTGCCGCGGAGGAGGGGAACAAAGTGGTAGACTTGAAGAAGAACGAATATCAGTACCAGCCACCGAGGTACCCCGTGGAGATGTTTGCTTATCCACCGCAGATATTCAGTGATGAAAACCCCAATGCTTGTACCATTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

283

Amino Acids

32.08

Weight (kDa)

8.0

Isoelectric Point (pI)

48.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 31 - 82 9.9e-12 Heavy-metal-associated domain
HMA PF00403 128 - 184 5.7e-13 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 777
AccB1I GGYRCC 1 cut(s) 777
AccI GTMKAC 1 cut(s) 738
AccII CGCG 2 cut(s) 560, 716
AccIII TCCGGA 2 cut(s) 289, 592
AciI CCGC 7 cut(s) 558, 560, 578, 581, 714, 716, 806
AclWI GGATC 3 cut(s) 215, 440, 569
AcuI CTGAAG 1 cut(s) 39
AfaI GTAC 3 cut(s) 764, 779, 841
AfiI CCNNNNNNNGG 1 cut(s) 656
AflII CTTAAG 1 cut(s) 92
AgeI ACCGGT 1 cut(s) 301
AgsI TTSAA 3 cut(s) 155, 425, 745
AhlI ACTAGT 1 cut(s) 188
AluBI AGCT 2 cut(s) 274, 520
AluI AGCT 2 cut(s) 274, 520
AlwI GGATC 3 cut(s) 215, 440, 569
Aor13HI TCCGGA 2 cut(s) 289, 592
AoxI GGCC 1 cut(s) 666
ApeKI GCWGC 1 cut(s) 710
AsiGI ACCGGT 1 cut(s) 301
Asp718I GGTACC 1 cut(s) 777
AsuHPI GGTGA 2 cut(s) 214, 273
AxyI CCTNAGG 1 cut(s) 348
BaeI ACNNNNGTAYC 2 cut(s) 761, 794
BanI GGYRCC 1 cut(s) 777
BbvI GCAGC 1 cut(s) 697
BccI CCATC 2 cut(s) 267, 698
BcgI CGANNNNNNTGC 2 cut(s) 692, 726
BcuI ACTAGT 1 cut(s) 188
BfaI CTAG 2 cut(s) 189, 521
BfrI CTTAAG 1 cut(s) 92
BisI GCNGC 4 cut(s) 558, 581, 711, 714
BlsI GCNGC 4 cut(s) 559, 582, 712, 715
BmiI GGNNCC 3 cut(s) 294, 465, 779
BmsI GCATC 1 cut(s) 488
BplI GAGNNNNNCTC 2 cut(s) 263, 295
BpuEI CTTGAG 1 cut(s) 63
BsaJI CCNNGG 6 cut(s) 414, 558, 669, 714, 773, 783
BsaWI WCCGGW 4 cut(s) 289, 301, 316, 592
Bsc4I CCNNNNNNNGG 1 cut(s) 656
Bse118I RCCGGY 1 cut(s) 301
Bse21I CCTNAGG 1 cut(s) 348
BseAI TCCGGA 2 cut(s) 289, 592
BseDI CCNNGG 6 cut(s) 414, 558, 669, 714, 773, 783
BseGI GGATG 1 cut(s) 172
BseLI CCNNNNNNNGG 1 cut(s) 656
BseMII CTCAG 2 cut(s) 339, 374
BseRI GAGGAG 4 cut(s) 20, 365, 702, 734
BseXI GCAGC 1 cut(s) 697
Bsh1236I CGCG 2 cut(s) 560, 716
BshFI GGCC 1 cut(s) 668
BshNI GGYRCC 1 cut(s) 777
BshTI ACCGGT 1 cut(s) 301
BsiSI CCGG 5 cut(s) 290, 302, 317, 512, 593
BslFI GGGAC 1 cut(s) 657
BslI CCNNNNNNNGG 1 cut(s) 656
BsmFI GGGAC 1 cut(s) 657
BsnI GGCC 1 cut(s) 668
Bsp13I TCCGGA 2 cut(s) 289, 592
Bsp143I GATC 3 cut(s) 220, 432, 574
Bsp19I CCATGG 1 cut(s) 414
BspACI CCGC 7 cut(s) 558, 560, 578, 581, 714, 716, 806
BspANI GGCC 1 cut(s) 668
BspCNI CTCAG 2 cut(s) 340, 375
BspEI TCCGGA 2 cut(s) 289, 592
BspFNI CGCG 2 cut(s) 560, 716
BspHI TCATGA 1 cut(s) 435
BspLI GGNNCC 3 cut(s) 294, 465, 779
BspPI GGATC 3 cut(s) 215, 440, 569
BspQI GCTCTTC 2 cut(s) 15, 248
BspT107I GGYRCC 1 cut(s) 777
BspTI CTTAAG 1 cut(s) 92
BsrFI RCCGGY 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 6 cut(s) 414, 558, 669, 714, 773, 783
BssMI GATC 3 cut(s) 220, 432, 574
BssT1I CCWWGG 2 cut(s) 414, 669
Bst4CI ACNGT 2 cut(s) 376, 680
Bst6I CTCTTC 5 cut(s) 15, 54, 248, 357, 633
BstAFI CTTAAG 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 244
BstC8I GCNNGC 1 cut(s) 555
BstDEI CTNAG 2 cut(s) 348, 383
BstDSI CCRYGG 4 cut(s) 414, 558, 714, 783
BstF5I GGATG 1 cut(s) 172
BstFNI CGCG 2 cut(s) 560, 716
BstKTI GATC 3 cut(s) 223, 435, 577
BstMBI GATC 3 cut(s) 220, 432, 574
BstMWI GCNNNNNNNGC 3 cut(s) 244, 517, 577
BstNSI RCATGY 3 cut(s) 397, 411, 557
BstUI CGCG 2 cut(s) 560, 716
BstV1I GCAGC 1 cut(s) 697
BstX2I RGATCY 2 cut(s) 220, 574
BstYI RGATCY 2 cut(s) 220, 574
Bsu36I CCTNAGG 1 cut(s) 348
BsuRI GGCC 1 cut(s) 668
BtgI CCRYGG 4 cut(s) 414, 558, 714, 783
BtgZI GCGATG 1 cut(s) 717
BtsCI GGATG 1 cut(s) 172
BtsIMutI CAGTG 2 cut(s) 381, 823
Cac8I GCNNGC 1 cut(s) 555
CciI TCATGA 1 cut(s) 435
Cfr10I RCCGGY 1 cut(s) 301
Cfr42I CCGCGG 2 cut(s) 561, 717
Csp6I GTAC 3 cut(s) 763, 778, 840
CspAI ACCGGT 1 cut(s) 301
CviAII CATG 5 cut(s) 394, 408, 415, 436, 554
CviJI RGCY 9 cut(s) 25, 150, 258, 274, 295, 520, 649, 668, 769
CviKI_1 RGCY 9 cut(s) 25, 150, 258, 274, 295, 520, 649, 668, 769
CviQI GTAC 3 cut(s) 763, 778, 840
DdeI CTNAG 2 cut(s) 348, 383
DpnI GATC 3 cut(s) 222, 434, 576
DpnII GATC 3 cut(s) 220, 432, 574
DraI TTTAAA 1 cut(s) 475
Eam1104I CTCTTC 5 cut(s) 15, 54, 248, 357, 633
EarI CTCTTC 5 cut(s) 15, 54, 248, 357, 633
EciI GGCGGA 1 cut(s) 567
Eco130I CCWWGG 2 cut(s) 414, 669
Eco57I CTGAAG 1 cut(s) 39
Eco81I CCTNAGG 1 cut(s) 348
EcoT14I CCWWGG 2 cut(s) 414, 669
EcoT22I ATGCAT 2 cut(s) 108, 399
ErhI CCWWGG 2 cut(s) 414, 669
FaeI CATG 5 cut(s) 397, 411, 418, 439, 557
FaiI YATR 9 cut(s) 104, 135, 395, 409, 416, 437, 555, 848, 850
FalI AAGNNNNNCTT 2 cut(s) 258, 290
FaqI GGGAC 1 cut(s) 657
FatI CATG 5 cut(s) 393, 407, 414, 435, 553
FblI GTMKAC 1 cut(s) 738
Fnu4HI GCNGC 4 cut(s) 558, 581, 711, 714
FokI GGATG 1 cut(s) 179
Fsp4HI GCNGC 4 cut(s) 558, 581, 711, 714
FspBI CTAG 2 cut(s) 189, 521
GluI GCNGC 4 cut(s) 558, 581, 711, 714
HaeIII GGCC 1 cut(s) 668
HapII CCGG 5 cut(s) 290, 302, 317, 512, 593
Hin1II CATG 5 cut(s) 397, 411, 418, 439, 557
HinfI GANTC 4 cut(s) 286, 421, 455, 543
HpaII CCGG 5 cut(s) 290, 302, 317, 512, 593
HphI GGTGA 2 cut(s) 214, 273
Hpy166II GTNNAC 2 cut(s) 391, 739
Hpy188I TCNGA 3 cut(s) 19, 40, 310
Hpy188III TCNNGA 5 cut(s) 80, 290, 436, 547, 593
Hpy8I GTNNAC 2 cut(s) 391, 739
HpyAV CCTTC 5 cut(s) 107, 149, 208, 325, 650
HpyCH4III ACNGT 2 cut(s) 376, 680
HpyCH4V TGCA 4 cut(s) 106, 238, 247, 397
HpyF10VI GCNNNNNNNGC 3 cut(s) 244, 517, 577
HpyF3I CTNAG 2 cut(s) 348, 383
Hsp92II CATG 5 cut(s) 397, 411, 418, 439, 557
Kpn2I TCCGGA 2 cut(s) 289, 592
KpnI GGTACC 1 cut(s) 781
KspI CCGCGG 2 cut(s) 561, 717
Kzo9I GATC 3 cut(s) 220, 432, 574
LguI GCTCTTC 2 cut(s) 15, 248
LmnI GCTCC 2 cut(s) 271, 292
LpnPI CCDG 9 cut(s) 66, 303, 315, 330, 360, 480, 525, 606, 779
Lsp1109I GCAGC 1 cut(s) 697
LweI GCATC 1 cut(s) 488
MaeI CTAG 2 cut(s) 189, 521
MaeIII GTNAC 2 cut(s) 169, 370
MalI GATC 3 cut(s) 222, 434, 576
MboI GATC 3 cut(s) 220, 432, 574
MflI RGATCY 2 cut(s) 220, 574
MlyI GAGTC 1 cut(s) 464
Mph1103I ATGCAT 2 cut(s) 108, 399
MroI TCCGGA 2 cut(s) 289, 592
MseI TTAA 4 cut(s) 93, 144, 227, 474
MslI CAYNNNNRTG 1 cut(s) 440
MspA1I CMGCKG 2 cut(s) 560, 716
MspCI CTTAAG 1 cut(s) 92
MspI CCGG 5 cut(s) 290, 302, 317, 512, 593
MvnI CGCG 2 cut(s) 560, 716
MwoI GCNNNNNNNGC 3 cut(s) 244, 517, 577
NcoI CCATGG 1 cut(s) 414
NdeII GATC 3 cut(s) 220, 432, 574
NlaIII CATG 5 cut(s) 397, 411, 418, 439, 557
NlaIV GGNNCC 3 cut(s) 294, 465, 779
NmuCI GTSAC 1 cut(s) 370
NsiI ATGCAT 2 cut(s) 108, 399
NspI RCATGY 3 cut(s) 397, 411, 557
PaeI GCATGC 1 cut(s) 557
PagI TCATGA 1 cut(s) 435
PciSI GCTCTTC 2 cut(s) 15, 248
PfeI GAWTC 3 cut(s) 286, 421, 543
PinAI ACCGGT 1 cut(s) 301
PkrI GCNGC 4 cut(s) 559, 582, 712, 715
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PspN4I GGNNCC 3 cut(s) 294, 465, 779
PsuI RGATCY 2 cut(s) 220, 574
RsaI GTAC 3 cut(s) 764, 779, 841
RsaNI GTAC 3 cut(s) 763, 778, 840
RseI CAYNNNNRTG 1 cut(s) 440
SacII CCGCGG 2 cut(s) 561, 717
SapI GCTCTTC 2 cut(s) 15, 248
SaqAI TTAA 4 cut(s) 93, 144, 227, 474
SatI GCNGC 4 cut(s) 558, 581, 711, 714
Sau3AI GATC 3 cut(s) 220, 432, 574
SchI GAGTC 1 cut(s) 464
SfaNI GCATC 1 cut(s) 488
Sfr303I CCGCGG 2 cut(s) 561, 717
SgrBI CCGCGG 2 cut(s) 561, 717
SmiMI CAYNNNNRTG 1 cut(s) 440
SmlI CTYRAG 2 cut(s) 78, 92
SmoI CTYRAG 2 cut(s) 78, 92
SpeI ACTAGT 1 cut(s) 188
SphI GCATGC 1 cut(s) 557
SsiI CCGC 7 cut(s) 558, 560, 578, 581, 714, 716, 806
SspMI CTAG 2 cut(s) 189, 521
StyI CCWWGG 2 cut(s) 414, 669
TaaI ACNGT 2 cut(s) 376, 680
TauI GCSGC 3 cut(s) 560, 583, 716
TfiI GAWTC 3 cut(s) 286, 421, 543
Tru1I TTAA 4 cut(s) 93, 144, 227, 474
Tru9I TTAA 4 cut(s) 93, 144, 227, 474
TscAI CASTG 2 cut(s) 381, 823
TseFI GTSAC 1 cut(s) 370
TseI GCWGC 1 cut(s) 710
Tsp45I GTSAC 1 cut(s) 370
TspDTI ATGAA 4 cut(s) 327, 458, 520, 837
TspRI CASTG 2 cut(s) 381, 823
Vha464I CTTAAG 1 cut(s) 92
XceI RCATGY 3 cut(s) 397, 411, 557
XmiI GTMKAC 1 cut(s) 738
XspI CTAG 2 cut(s) 189, 521
Zsp2I ATGCAT 2 cut(s) 108, 399
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.