pycom05g32740

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002139
Physical Location & Seq
Forward (+)
32020411 .. 32024348
3938 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g32740.3

Sequence Viewer

Length: 810 bp
ATGGGCGAAGAGAAGAAAGAGGAAGAGAAGAAGGAAGAAAGCAAAGATGAAAAGAAAGAAGAGAAGAAGGAAGAAAAGAAAGAAGAAGAGCCTCCGGAGATTGTACTCAAGGTTGATATGCATTGTGAAGCTTGCGCAAGGAAAGTTGCTCGAGCCTTGAAGGGTTTTGAAGGAGTGGAGGATGTAACGACAGACAGCAAGACGAGTAAGGTGGTGGTGAAAGGGAAGGCGGCAGACCCCATAAAGGTCTGCGAAAGATTACAAAAGAAAAGCGGCAAAAAAGTGGAGCTCATTTCACCTTTGCCTAAACCACCAGAGGAGAAGAAGGAGGACCAACCGGTTAAAGAAGCTGACAAGGAAGAGAAAAAGGAGGAGCCTCCTGCTGTTGTTACAGTGGTATTGCAAGTTCGAATGCACTGCGAAGCGTGCGCTCAAGTTCTACAGAAGCGAATTCGAAAGATCCAAGGAGTGGAATCAGTGGAAACAGACGTAGCTAATAATGAGGTTGTAGTAAAAGGCGTAGTGGACCCGGCGAAGCTTGCTGAGGAGGTGTATAAAAAAACCAGGAAGCAGGTTTCTGTTGTGAAGGAGGAAGAGAAGAAGGAAGAAGAAAAGAAAGAAGAGGAGAAGAAAGAAGCCGAGAAAGAAGGAGAGACGAAACAAGGGGAGGAAGACAAGCAGGGAGAGGATAATAAGGTCGATATCAAGCGAAGTGAATATTGGCCGACAAAGTACTACTCGGATTATTCATCGTATCCAAACCCTCAGATTTTCAGCGATGAAAACCCTAATGCATGCTCTGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

270

Amino Acids

30.78

Weight (kDa)

5.3

Isoelectric Point (pI)

59.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 562
AccB7I CCANNNNNTGG 1 cut(s) 469
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 2 cut(s) 230, 273
AclWI GGATC 1 cut(s) 454
AcoI YGGCCR 1 cut(s) 722
AcsI RAATTY 1 cut(s) 450
AfaI GTAC 2 cut(s) 105, 734
AfiI CCNNNNNNNGG 2 cut(s) 244, 469
AgeI ACCGGT 1 cut(s) 337
AgsI TTSAA 2 cut(s) 160, 170
AjnI CCWGG 1 cut(s) 563
AjuI GAANNNNNNNTTGG 2 cut(s) 703, 735
AluBI AGCT 5 cut(s) 131, 289, 350, 494, 538
AluI AGCT 5 cut(s) 131, 289, 350, 494, 538
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 1 cut(s) 647
AlwI GGATC 1 cut(s) 454
Ama87I CYCGRG 1 cut(s) 150
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 722
ApoI RAATTY 1 cut(s) 450
AsiGI ACCGGT 1 cut(s) 337
AspLEI GCGC 2 cut(s) 137, 431
AspS9I GGNCC 2 cut(s) 331, 526
AsuC2I CCSGG 1 cut(s) 530
AsuHPI GGTGA 2 cut(s) 229, 288
AsuII TTCGAA 2 cut(s) 409, 454
AvaI CYCGRG 1 cut(s) 150
AvaII GGWCC 2 cut(s) 331, 526
BanII GRGCYC 1 cut(s) 291
BbsI GAAGAC 1 cut(s) 678
Bbv12I GWGCWC 1 cut(s) 291
BbvCI CCTCAGC 1 cut(s) 543
BcgI CGANNNNNNTGC 2 cut(s) 399, 433
BciT130I CCWGG 1 cut(s) 565
BciVI GTATCC 1 cut(s) 765
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 647
BfmI CTRYAG 1 cut(s) 440
BfuAI ACCTGC 1 cut(s) 562
BfuI GTATCC 1 cut(s) 765
BisI GCNGC 2 cut(s) 231, 274
BlsI GCNGC 2 cut(s) 232, 275
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 2 cut(s) 530, 565
Bme18I GGWCC 2 cut(s) 331, 526
BmeT110I CYCGRG 1 cut(s) 150
BmgT120I GGNCC 2 cut(s) 331, 526
BmiI GGNNCC 2 cut(s) 375, 528
BmrFI CCNGG 2 cut(s) 530, 565
BpiI GAAGAC 1 cut(s) 678
Bpu10I CCTNAGC 1 cut(s) 543
Bpu14I TTCGAA 2 cut(s) 409, 454
BpuEI CTTGAG 2 cut(s) 92, 417
BpuMI CCSGG 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 463
BsaWI WCCGGW 2 cut(s) 94, 337
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 469
Bse118I RCCGGY 1 cut(s) 337
BseAI TCCGGA 1 cut(s) 94
BseBI CCWGG 1 cut(s) 565
BseDI CCNNGG 1 cut(s) 463
BseGI GGATG 1 cut(s) 187
BseLI CCNNNNNNNGG 2 cut(s) 244, 469
BseMII CTCAG 2 cut(s) 534, 779
BseRI GAGGAG 4 cut(s) 332, 386, 560, 638
BshFI GGCC 1 cut(s) 724
BshTI ACCGGT 1 cut(s) 337
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 3 cut(s) 95, 338, 530
BslI CCNNNNNNNGG 2 cut(s) 244, 469
BsmAI GTCTC 1 cut(s) 647
BsmBI CGTCTC 1 cut(s) 647
BsmI GAATGC 1 cut(s) 417
BsnI GGCC 1 cut(s) 724
BsoBI CYCGRG 1 cut(s) 150
Bsp119I TTCGAA 2 cut(s) 409, 454
Bsp1286I GDGCHC 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 1 cut(s) 459
BspACI CCGC 2 cut(s) 230, 273
BspANI GGCC 1 cut(s) 724
BspCNI CTCAG 2 cut(s) 535, 778
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 375, 528
BspMI ACCTGC 1 cut(s) 562
BspPI GGATC 1 cut(s) 454
BspQI GCTCTTC 1 cut(s) 81
BspT104I TTCGAA 2 cut(s) 409, 454
BsrFI RCCGGY 1 cut(s) 337
BssAI RCCGGY 1 cut(s) 337
BssECI CCNNGG 1 cut(s) 463
BssMI GATC 1 cut(s) 459
BssT1I CCWWGG 1 cut(s) 463
Bst2UI CCWGG 1 cut(s) 565
Bst4CI ACNGT 1 cut(s) 394
Bst6I CTCTTC 7 cut(s) 3, 18, 54, 81, 354, 588, 615
BstBI TTCGAA 2 cut(s) 409, 454
BstC8I GCNNGC 4 cut(s) 133, 427, 540, 796
BstDEI CTNAG 2 cut(s) 543, 765
BstF5I GGATG 1 cut(s) 187
BstHHI GCGC 2 cut(s) 137, 431
BstKTI GATC 1 cut(s) 462
BstMAI GTCTC 1 cut(s) 647
BstMBI GATC 1 cut(s) 459
BstMWI GCNNNNNNNGC 2 cut(s) 426, 539
BstNI CCWGG 1 cut(s) 565
BstNSI RCATGY 1 cut(s) 798
BstSCI CCNGG 2 cut(s) 528, 563
BstSFI CTRYAG 1 cut(s) 440
BstV2I GAAGAC 1 cut(s) 678
BstX2I RGATCY 1 cut(s) 459
BstYI RGATCY 1 cut(s) 459
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 1 cut(s) 724
BtgZI GCGATG 1 cut(s) 792
BtsCI GGATG 1 cut(s) 187
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 3 cut(s) 399, 415, 483
BveI ACCTGC 1 cut(s) 562
Cac8I GCNNGC 4 cut(s) 133, 427, 540, 796
CfoI GCGC 2 cut(s) 137, 431
Cfr10I RCCGGY 1 cut(s) 337
Cfr13I GGNCC 2 cut(s) 331, 526
Csp6I GTAC 2 cut(s) 104, 733
CspAI ACCGGT 1 cut(s) 337
CviAII CATG 1 cut(s) 795
CviQI GTAC 2 cut(s) 104, 733
DdeI CTNAG 2 cut(s) 543, 765
DpnI GATC 1 cut(s) 461
DpnII GATC 1 cut(s) 459
EaeI YGGCCR 1 cut(s) 722
Eam1104I CTCTTC 7 cut(s) 3, 18, 54, 81, 354, 588, 615
EarI CTCTTC 7 cut(s) 3, 18, 54, 81, 354, 588, 615
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 1 cut(s) 463
Eco24I GRGCYC 1 cut(s) 291
Eco32I GATATC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 331, 526
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 1 cut(s) 150
EcoICRI GAGCTC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 450
EcoRII CCWGG 1 cut(s) 563
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 1 cut(s) 463
EcoT22I ATGCAT 2 cut(s) 123, 796
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 1 cut(s) 463
Esp3I CGTCTC 1 cut(s) 647
FaeI CATG 1 cut(s) 798
FaiI YATR 5 cut(s) 119, 242, 555, 796, 806
FatI CATG 1 cut(s) 794
Fnu4HI GCNGC 2 cut(s) 231, 274
FokI GGATG 1 cut(s) 194
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 2 cut(s) 231, 274
FspI TGCGCA 1 cut(s) 136
GlaI GCGC 2 cut(s) 136, 430
GluI GCNGC 2 cut(s) 231, 274
HaeIII GGCC 1 cut(s) 724
HapII CCGG 3 cut(s) 95, 338, 530
HhaI GCGC 2 cut(s) 137, 431
Hin1II CATG 1 cut(s) 798
Hin6I GCGC 2 cut(s) 135, 429
HinP1I GCGC 2 cut(s) 135, 429
HindIII AAGCTT 2 cut(s) 129, 536
HinfI GANTC 1 cut(s) 473
HpaII CCGG 3 cut(s) 95, 338, 530
HphI GGTGA 2 cut(s) 229, 288
Hpy166II GTNNAC 1 cut(s) 526
Hpy188I TCNGA 2 cut(s) 742, 768
Hpy188III TCNNGA 1 cut(s) 95
Hpy8I GTNNAC 1 cut(s) 526
HpyAV CCTTC 9 cut(s) 25, 61, 154, 164, 220, 319, 580, 595, 641
HpyCH4III ACNGT 1 cut(s) 394
HpyCH4IV ACGT 1 cut(s) 489
HpyCH4V TGCA 4 cut(s) 121, 403, 415, 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 426, 539
HpyF3I CTNAG 2 cut(s) 543, 765
HpySE526I ACGT 1 cut(s) 489
Hsp92II CATG 1 cut(s) 798
HspAI GCGC 2 cut(s) 135, 429
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 459
LguI GCTCTTC 1 cut(s) 81
LmnI GCTCC 2 cut(s) 286, 373
LpnPI CCDG 9 cut(s) 108, 327, 351, 393, 543, 550, 557, 577, 665
MaeII ACGT 1 cut(s) 489
MaeIII GTNAC 2 cut(s) 184, 388
MalI GATC 1 cut(s) 461
MboI GATC 1 cut(s) 459
MflI RGATCY 1 cut(s) 459
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 1 cut(s) 450
Mph1103I ATGCAT 2 cut(s) 123, 796
MroI TCCGGA 1 cut(s) 94
MseI TTAA 1 cut(s) 342
MspI CCGG 3 cut(s) 95, 338, 530
MspR9I CCNGG 2 cut(s) 530, 565
Mva1269I GAATGC 1 cut(s) 417
MvaI CCWGG 1 cut(s) 565
MwoI GCNNNNNNNGC 2 cut(s) 426, 539
NciI CCSGG 1 cut(s) 530
NdeII GATC 1 cut(s) 459
NlaIII CATG 1 cut(s) 798
NlaIV GGNNCC 2 cut(s) 375, 528
NmeAIII GCCGAG 1 cut(s) 664
NsbI TGCGCA 1 cut(s) 136
NsiI ATGCAT 2 cut(s) 123, 796
NspI RCATGY 1 cut(s) 798
NspV TTCGAA 2 cut(s) 409, 454
PaeI GCATGC 1 cut(s) 798
PaeR7I CTCGAG 1 cut(s) 150
PciSI GCTCTTC 1 cut(s) 81
PctI GAATGC 1 cut(s) 417
PfeI GAWTC 1 cut(s) 473
PflMI CCANNNNNTGG 1 cut(s) 469
PinAI ACCGGT 1 cut(s) 337
PkrI GCNGC 2 cut(s) 232, 275
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 563
PspGI CCWGG 1 cut(s) 563
PspN4I GGNNCC 2 cut(s) 375, 528
PspPI GGNCC 2 cut(s) 331, 526
PspXI VCTCGAGB 1 cut(s) 150
PsrI GAACNNNNNNTAC 2 cut(s) 390, 422
PsuI RGATCY 1 cut(s) 459
RsaI GTAC 2 cut(s) 105, 734
RsaNI GTAC 2 cut(s) 104, 733
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 1 cut(s) 81
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 2 cut(s) 231, 274
Sau3AI GATC 1 cut(s) 459
Sau96I GGNCC 2 cut(s) 331, 526
ScaI AGTACT 1 cut(s) 734
ScrFI CCNGG 2 cut(s) 530, 565
SduI GDGCHC 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 440
Sfr274I CTCGAG 1 cut(s) 150
SfuI TTCGAA 2 cut(s) 409, 454
SinI GGWCC 2 cut(s) 331, 526
SlaI CTCGAG 1 cut(s) 150
SmlI CTYRAG 3 cut(s) 107, 150, 432
SmoI CTYRAG 3 cut(s) 107, 150, 432
SphI GCATGC 1 cut(s) 798
Sse9I AATT 1 cut(s) 450
SsiI CCGC 2 cut(s) 230, 273
SspI AATATT 1 cut(s) 719
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 528, 563
StyI CCWWGG 1 cut(s) 463
TaaI ACNGT 1 cut(s) 394
TaiI ACGT 1 cut(s) 492
TaqI TCGA 4 cut(s) 151, 409, 454, 699
TasI AATT 1 cut(s) 450
TatI WGTACW 2 cut(s) 103, 732
TauI GCSGC 2 cut(s) 233, 276
TfiI GAWTC 1 cut(s) 473
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 3 cut(s) 399, 422, 483
TspDTI ATGAA 3 cut(s) 63, 738, 795
TspRI CASTG 3 cut(s) 399, 422, 483
Van91I CCANNNNNTGG 1 cut(s) 469
VpaK11BI GGWCC 2 cut(s) 331, 526
XapI RAATTY 1 cut(s) 450
XceI RCATGY 1 cut(s) 798
XhoI CTCGAG 1 cut(s) 150
ZrmI AGTACT 1 cut(s) 734
Zsp2I ATGCAT 2 cut(s) 123, 796
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.