Rh7CG109200

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0002139
Physical Location & Seq
Reverse (-)
8427127 .. 8429831
2705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG109200.1

Sequence Viewer

Length: 765 bp
ATGGTGTTTGGTTTTTCAGGGGTGGAAGATGTGAGCACTGATTGCAAGAGTCACAAGGTGGTTGTCAAGGGCGAGAAGGCTGATCCAAGCAAGGTTCTCGAGAGAGTTCAGAGGAAGAGTCACAGACAAGTTGAGCTTCTCTCCCCAATCCCAAAACCACCAGCTGAGGAGGAGAAGAAGCCTGAAGAGAAACCCAAGCCAGAGGAGAAGAAGGAAGAGCCTCCTCAGGTCATCACTGTGGTGCTCAAGGTTCACATGCATTGCGAGGCTTGTGCACAAGAAATCAAGAAACGCATACAGAGGATGAAAGGAGTGGAATCAGCAGAACCAGATCTAAAGGGCTCACAGGTGACAGTTAAGGGTGTGTTTGATCCGGCCAAGCTAGTTGAGTACGTGAACAAAAGGACCGGCAAGCACGCAGAGATAGTGAAGCAAGAACCGGAAAAGAAGGCTGACAAAGAAGAAGCCAAAGAGGGAAGTAAAGACGAGAAGAAAGGTGGTGAAGAAGGTGACAAGGACAAGAAGAGTGGTGGTGGAGGTGAGGCCGCACCAGCAGAAGAGAACAAAGACAAGAAAAGTGACGGCAATGAAGGCGGTGCTGCAGCTGCTGCTACACCGGATGGCGGGGCGGTTACAGAAGAGACCAAGGTTGTTGAGATCATGAAGAACGAGTACAATTACTATGCCCAAAGACTTCCCATGGAGTACGCCTACCCTCCACAGTTCTTCAGTGATGAGAACCCCAATGCATGTTCTGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

254

Amino Acids

27.98

Weight (kDa)

5.77

Isoelectric Point (pI)

47.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 82 - 138 2.9e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 546, 594, 624, 629
AclWI GGATC 2 cut(s) 77, 365
AcoI YGGCCR 1 cut(s) 375
AcuI CTGAAG 2 cut(s) 204, 712
AdeI CACNNNGTG 1 cut(s) 58
AfaI GTAC 3 cut(s) 392, 674, 707
AfiI CCNNNNNNNGG 1 cut(s) 623
AleI CACNNNNGTG 1 cut(s) 239
AluBI AGCT 4 cut(s) 136, 164, 382, 605
AluI AGCT 4 cut(s) 136, 164, 382, 605
Alw21I GWGCWC 3 cut(s) 38, 246, 277
Alw26I GTCTC 1 cut(s) 635
Alw44I GTGCAC 1 cut(s) 273
AlwI GGATC 2 cut(s) 77, 365
AlwNI CAGNNNCTG 1 cut(s) 608
Ama87I CYCGRG 1 cut(s) 98
AoxI GGCC 2 cut(s) 375, 543
ApaLI GTGCAC 1 cut(s) 273
ApeKI GCWGC 4 cut(s) 599, 602, 605, 608
AspS9I GGNCC 1 cut(s) 405
AsuHPI GGTGA 4 cut(s) 361, 512, 521, 551
AvaI CYCGRG 1 cut(s) 98
AvaII GGWCC 1 cut(s) 405
AxyI CCTNAGG 1 cut(s) 225
BaeGI GKGCMC 1 cut(s) 277
BanII GRGCYC 1 cut(s) 344
BarI GAAGNNNNNNTAC 2 cut(s) 656, 688
Bbv12I GWGCWC 3 cut(s) 38, 246, 277
BbvCI CCTCAGC 1 cut(s) 165
BbvI GCAGC 4 cut(s) 586, 592, 595, 614
BccI CCATC 1 cut(s) 614
BceAI ACGGC 1 cut(s) 598
BcgI CGANNNNNNTGC 4 cut(s) 79, 113, 254, 288
BcoDI GTCTC 1 cut(s) 635
BfaI CTAG 1 cut(s) 383
BfmI CTRYAG 1 cut(s) 600
BglII AGATCT 1 cut(s) 331
BisI GCNGC 5 cut(s) 546, 600, 603, 606, 609
BlsI GCNGC 5 cut(s) 547, 601, 604, 607, 610
Bme18I GGWCC 1 cut(s) 405
BmeT110I CYCGRG 1 cut(s) 98
BmgT120I GGNCC 1 cut(s) 405
BplI GAGNNNNNCTC 2 cut(s) 125, 157
Bpu10I CCTNAGC 1 cut(s) 165
BpuEI CTTGAG 1 cut(s) 230
BsaAI YACGTR 1 cut(s) 394
BsaI GGTCTC 1 cut(s) 635
BsaJI CCNNGG 2 cut(s) 645, 699
BsaWI WCCGGW 2 cut(s) 439, 616
Bsc4I CCNNNNNNNGG 1 cut(s) 623
Bse118I RCCGGY 1 cut(s) 407
Bse21I CCTNAGG 1 cut(s) 225
Bse3DI GCAATG 2 cut(s) 259, 592
BseDI CCNNGG 2 cut(s) 645, 699
BseGI GGATG 2 cut(s) 309, 625
BseLI CCNNNNNNNGG 1 cut(s) 623
BseMI GCAATG 2 cut(s) 259, 592
BseMII CTCAG 2 cut(s) 156, 239
BseRI GAGGAG 4 cut(s) 182, 185, 213, 218
BseSI GKGCMC 1 cut(s) 277
BseXI GCAGC 4 cut(s) 586, 592, 595, 614
BshFI GGCC 2 cut(s) 377, 545
BsiHKAI GWGCWC 3 cut(s) 38, 246, 277
BsiHKCI CYCGRG 1 cut(s) 98
BsiSI CCGG 4 cut(s) 374, 408, 440, 617
BslI CCNNNNNNNGG 1 cut(s) 623
BsmAI GTCTC 1 cut(s) 635
BsnI GGCC 2 cut(s) 377, 545
Bso31I GGTCTC 1 cut(s) 635
BsoBI CYCGRG 1 cut(s) 98
Bsp1286I GDGCHC 4 cut(s) 38, 246, 277, 344
Bsp143I GATC 4 cut(s) 82, 331, 370, 657
Bsp19I CCATGG 1 cut(s) 699
BspACI CCGC 4 cut(s) 546, 594, 624, 629
BspANI GGCC 2 cut(s) 377, 545
BspCNI CTCAG 2 cut(s) 157, 238
BspHI TCATGA 1 cut(s) 660
BspMAI CTGCAG 1 cut(s) 604
BspPI GGATC 2 cut(s) 77, 365
BspQI GCTCTTC 1 cut(s) 210
BspTNI GGTCTC 1 cut(s) 635
BsrDI GCAATG 2 cut(s) 259, 592
BsrFI RCCGGY 1 cut(s) 407
BssAI RCCGGY 1 cut(s) 407
BssECI CCNNGG 2 cut(s) 645, 699
BssMI GATC 4 cut(s) 82, 331, 370, 657
BssT1I CCWWGG 2 cut(s) 645, 699
Bst4CI ACNGT 3 cut(s) 238, 355, 723
Bst6I CTCTTC 6 cut(s) 110, 180, 210, 518, 552, 633
BstAPI GCANNNNNTGC 2 cut(s) 42, 608
BstBAI YACGTR 1 cut(s) 394
BstC8I GCNNGC 2 cut(s) 413, 417
BstDEI CTNAG 2 cut(s) 165, 225
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 2 cut(s) 309, 625
BstKTI GATC 4 cut(s) 85, 334, 373, 660
BstMAI GTCTC 1 cut(s) 635
BstMBI GATC 4 cut(s) 82, 331, 370, 657
BstMWI GCNNNNNNNGC 5 cut(s) 42, 551, 591, 605, 608
BstNSI RCATGY 2 cut(s) 259, 753
BstSFI CTRYAG 1 cut(s) 600
BstSLI GKGCMC 1 cut(s) 277
BstV1I GCAGC 4 cut(s) 586, 592, 595, 614
BstX2I RGATCY 1 cut(s) 331
BstYI RGATCY 1 cut(s) 331
Bsu36I CCTNAGG 1 cut(s) 225
BsuRI GGCC 2 cut(s) 377, 545
BtgI CCRYGG 1 cut(s) 699
BtsCI GGATG 2 cut(s) 309, 625
BtsIMutI CAGTG 3 cut(s) 36, 234, 736
Cac8I GCNNGC 2 cut(s) 413, 417
CaiI CAGNNNCTG 1 cut(s) 608
CciI TCATGA 1 cut(s) 660
Cfr10I RCCGGY 1 cut(s) 407
Cfr13I GGNCC 1 cut(s) 405
Csp6I GTAC 3 cut(s) 391, 673, 706
CspCI CAANNNNNGTGG 2 cut(s) 508, 543
CviAII CATG 4 cut(s) 256, 661, 700, 750
CviQI GTAC 3 cut(s) 391, 673, 706
DdeI CTNAG 2 cut(s) 165, 225
DpnI GATC 4 cut(s) 84, 333, 372, 659
DpnII GATC 4 cut(s) 82, 331, 370, 657
DraIII CACNNNGTG 1 cut(s) 58
EaeI YGGCCR 1 cut(s) 375
Eam1104I CTCTTC 6 cut(s) 110, 180, 210, 518, 552, 633
EarI CTCTTC 6 cut(s) 110, 180, 210, 518, 552, 633
Eco130I CCWWGG 2 cut(s) 645, 699
Eco24I GRGCYC 1 cut(s) 344
Eco31I GGTCTC 1 cut(s) 635
Eco47I GGWCC 1 cut(s) 405
Eco57I CTGAAG 2 cut(s) 204, 712
Eco81I CCTNAGG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 98
EcoT14I CCWWGG 2 cut(s) 645, 699
EcoT22I ATGCAT 2 cut(s) 261, 751
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 2 cut(s) 645, 699
FaeI CATG 4 cut(s) 259, 664, 703, 753
FaiI YATR 7 cut(s) 257, 296, 662, 684, 701, 751, 761
FalI AAGNNNNNCTT 2 cut(s) 120, 152
FatI CATG 4 cut(s) 255, 660, 699, 749
FauI CCCGC 1 cut(s) 617
Fnu4HI GCNGC 5 cut(s) 546, 600, 603, 606, 609
FokI GGATG 2 cut(s) 316, 632
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 5 cut(s) 546, 600, 603, 606, 609
FspBI CTAG 1 cut(s) 383
GluI GCNGC 5 cut(s) 546, 600, 603, 606, 609
HaeIII GGCC 2 cut(s) 377, 545
HapII CCGG 4 cut(s) 374, 408, 440, 617
Hin1II CATG 4 cut(s) 259, 664, 703, 753
HinfI GANTC 3 cut(s) 49, 118, 317
HpaII CCGG 4 cut(s) 374, 408, 440, 617
HphI GGTGA 4 cut(s) 361, 512, 521, 551
Hpy166II GTNNAC 3 cut(s) 253, 275, 397
Hpy188I TCNGA 1 cut(s) 111
Hpy188III TCNNGA 4 cut(s) 98, 100, 286, 661
Hpy8I GTNNAC 3 cut(s) 253, 275, 397
HpyAV CCTTC 5 cut(s) 70, 205, 442, 500, 584
HpyCH4III ACNGT 3 cut(s) 238, 355, 723
HpyCH4IV ACGT 1 cut(s) 393
HpyCH4V TGCA 5 cut(s) 45, 259, 275, 602, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 551, 591, 605, 608
HpyF3I CTNAG 2 cut(s) 165, 225
HpySE526I ACGT 1 cut(s) 393
Hsp92II CATG 4 cut(s) 259, 664, 703, 753
Kzo9I GATC 4 cut(s) 82, 331, 370, 657
LguI GCTCTTC 1 cut(s) 210
Lsp1109I GCAGC 4 cut(s) 586, 592, 595, 614
MaeI CTAG 1 cut(s) 383
MaeII ACGT 1 cut(s) 393
MaeIII GTNAC 6 cut(s) 50, 119, 349, 509, 578, 631
MalI GATC 4 cut(s) 84, 333, 372, 659
MboI GATC 4 cut(s) 82, 331, 370, 657
MflI RGATCY 1 cut(s) 331
MhlI GDGCHC 4 cut(s) 38, 246, 277, 344
MluCI AATT 1 cut(s) 676
MlyI GAGTC 2 cut(s) 58, 127
Mph1103I ATGCAT 2 cut(s) 261, 751
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 2 cut(s) 236, 239
MspA1I CMGCKG 2 cut(s) 164, 605
MspI CCGG 4 cut(s) 374, 408, 440, 617
MwoI GCNNNNNNNGC 5 cut(s) 42, 551, 591, 605, 608
NcoI CCATGG 1 cut(s) 699
NdeII GATC 4 cut(s) 82, 331, 370, 657
NlaIII CATG 4 cut(s) 259, 664, 703, 753
NmuCI GTSAC 5 cut(s) 50, 119, 349, 509, 578
NsiI ATGCAT 2 cut(s) 261, 751
NspI RCATGY 2 cut(s) 259, 753
OliI CACNNNNGTG 1 cut(s) 239
PaeR7I CTCGAG 1 cut(s) 98
PagI TCATGA 1 cut(s) 660
PciSI GCTCTTC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 317
PkrI GCNGC 5 cut(s) 547, 601, 604, 607, 610
PleI GAGTC 2 cut(s) 57, 126
PpsI GAGTC 2 cut(s) 57, 126
Ppu21I YACGTR 1 cut(s) 394
PspPI GGNCC 1 cut(s) 405
PstI CTGCAG 1 cut(s) 604
PstNI CAGNNNCTG 1 cut(s) 608
PsuI RGATCY 1 cut(s) 331
PvuII CAGCTG 2 cut(s) 164, 605
RsaI GTAC 3 cut(s) 392, 674, 707
RsaNI GTAC 3 cut(s) 391, 673, 706
RseI CAYNNNNRTG 2 cut(s) 236, 239
SapI GCTCTTC 1 cut(s) 210
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 5 cut(s) 546, 600, 603, 606, 609
Sau3AI GATC 4 cut(s) 82, 331, 370, 657
Sau96I GGNCC 1 cut(s) 405
SchI GAGTC 2 cut(s) 58, 127
SduI GDGCHC 4 cut(s) 38, 246, 277, 344
SfcI CTRYAG 1 cut(s) 600
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 405
SlaI CTCGAG 1 cut(s) 98
SmiMI CAYNNNNRTG 2 cut(s) 236, 239
SmlI CTYRAG 2 cut(s) 98, 245
SmoI CTYRAG 2 cut(s) 98, 245
Sse9I AATT 1 cut(s) 676
SsiI CCGC 4 cut(s) 546, 594, 624, 629
SspMI CTAG 1 cut(s) 383
StyI CCWWGG 2 cut(s) 645, 699
TaaI ACNGT 3 cut(s) 238, 355, 723
TaiI ACGT 1 cut(s) 396
TaqI TCGA 1 cut(s) 99
TasI AATT 1 cut(s) 676
TatI WGTACW 1 cut(s) 672
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 317
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TscAI CASTG 3 cut(s) 43, 241, 736
TseFI GTSAC 5 cut(s) 50, 119, 349, 509, 578
TseI GCWGC 4 cut(s) 599, 602, 605, 608
Tsp45I GTSAC 5 cut(s) 50, 119, 349, 509, 578
TspDTI ATGAA 3 cut(s) 320, 603, 677
TspRI CASTG 3 cut(s) 43, 241, 736
VneI GTGCAC 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 405
XceI RCATGY 2 cut(s) 259, 753
XhoI CTCGAG 1 cut(s) 98
XspI CTAG 1 cut(s) 383
Zsp2I ATGCAT 2 cut(s) 261, 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.