MD05G1360600.v1.1

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0002139
Physical Location & Seq
Forward (+)
47654211 .. 47658792
4582 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1360600.v1.1.491

Sequence Viewer

Length: 813 bp
ATGGGCGAAGAGAAGAAAGAGGAAGAGAAGAAGGAAGAAAGCAAAGATGAAAAGAAAGAAGAAGAGAAAAAGGAAGAAAAGAAAGAAGAAGAGCCTCCGGATATTGTACTCAAGGTTGATATGCATTGTGAAGCTTGCGCAAGGAAGGTTGCTCGAGCCTTGAAGGGTTTTGAAGGAGTGGAGGATGTAACGACAGACAGCAAGGCAAGTAAGGTGGTGGTGAAAGGGAAGGCGGCAGACCCCATAAAGGTCTGCGAAAGATTACAAAAGAAAAGCGGCAAAAAAGTGGACCTCATTTCACCTTTGCCTAAACCACCAGAGGAGAAGAAGGAGGACCAACAGGTTAAAGAAGCTGACAAGGAAGAGAAAAAAGAGCAGCCTCCTGCTGTTGTTACGGTGGTATTGCAAGTTCGAATGCACTGCGACGCCTGCGCTCAAGTTCTACAGAAGCGAATTCGAAAGATCCAAGGAGTGGAATCAGTGGAAACAGACGTAGCTAATAATCAGGTTGTGGTAAAAGGCGTAGTGGACCCGGCGAAGCTAGCTGAGGAGGTGTATAAAAAAACCAGGAAGCAGGTTTCTGTTGTGAAGGAGGAAGAGAAGAAGGAAGAAGAAAAGAAAGAAGAGGAGAAGAAAGAAGCCGAGAAAGAAGGAGAGACGAAAGAAGGGGAGGAAGACAAGCAGGGAGAGGATAATAAGGTCGATATCAAGCGAAGTGAATATTGGCCGACAAAGTACTACTCGGATTATTCATCGTATCCAAACCCTCAGATTTTCAGCGATGAAAACCCTAATGCATGCTCTGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

271

Amino Acids

30.87

Weight (kDa)

5.28

Isoelectric Point (pI)

56.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 37 - 91 3.3e-13 Heavy-metal-associated domain
HMA PF00403 135 - 187 4.4e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 139
Acc36I ACCTGC 1 cut(s) 565
AccB7I CCANNNNNTGG 1 cut(s) 472
AccIII TCCGGA 1 cut(s) 97
AciI CCGC 2 cut(s) 233, 276
AclWI GGATC 1 cut(s) 457
AcoI YGGCCR 1 cut(s) 725
AcsI RAATTY 1 cut(s) 453
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 2 cut(s) 108, 737
AfiI CCNNNNNNNGG 2 cut(s) 247, 472
AgsI TTSAA 2 cut(s) 163, 173
AjnI CCWGG 1 cut(s) 566
AjuI GAANNNNNNNTTGG 2 cut(s) 706, 738
AluBI AGCT 5 cut(s) 134, 353, 497, 541, 545
AluI AGCT 5 cut(s) 134, 353, 497, 541, 545
Alw26I GTCTC 1 cut(s) 650
AlwI GGATC 1 cut(s) 457
Ama87I CYCGRG 1 cut(s) 153
Aor13HI TCCGGA 1 cut(s) 97
AoxI GGCC 1 cut(s) 725
ApeKI GCWGC 1 cut(s) 376
ApoI RAATTY 1 cut(s) 453
AspLEI GCGC 2 cut(s) 140, 434
AspS9I GGNCC 3 cut(s) 289, 334, 529
AsuC2I CCSGG 1 cut(s) 533
AsuHPI GGTGA 2 cut(s) 232, 291
AsuII TTCGAA 2 cut(s) 412, 457
AsuNHI GCTAGC 1 cut(s) 541
AvaI CYCGRG 1 cut(s) 153
AvaII GGWCC 3 cut(s) 289, 334, 529
BbsI GAAGAC 1 cut(s) 681
BbvCI CCTCAGC 1 cut(s) 546
BbvI GCAGC 1 cut(s) 388
BcgI CGANNNNNNTGC 2 cut(s) 402, 436
BciT130I CCWGG 1 cut(s) 568
BciVI GTATCC 1 cut(s) 768
BcnI CCSGG 1 cut(s) 533
BcoDI GTCTC 1 cut(s) 650
BfaI CTAG 1 cut(s) 542
BfmI CTRYAG 1 cut(s) 443
BfuAI ACCTGC 1 cut(s) 565
BfuI GTATCC 1 cut(s) 768
BisI GCNGC 3 cut(s) 234, 277, 377
BlsI GCNGC 3 cut(s) 235, 278, 378
BmcAI AGTACT 1 cut(s) 737
Bme1390I CCNGG 2 cut(s) 533, 568
Bme18I GGWCC 3 cut(s) 289, 334, 529
BmeT110I CYCGRG 1 cut(s) 153
BmgT120I GGNCC 3 cut(s) 289, 334, 529
BmiI GGNNCC 1 cut(s) 531
BmrFI CCNGG 2 cut(s) 533, 568
BmtI GCTAGC 1 cut(s) 545
BpiI GAAGAC 1 cut(s) 681
Bpu10I CCTNAGC 1 cut(s) 546
Bpu14I TTCGAA 2 cut(s) 412, 457
BpuEI CTTGAG 2 cut(s) 95, 420
BpuMI CCSGG 1 cut(s) 533
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 1 cut(s) 466
BsaWI WCCGGW 1 cut(s) 97
Bsc4I CCNNNNNNNGG 2 cut(s) 247, 472
BseAI TCCGGA 1 cut(s) 97
BseBI CCWGG 1 cut(s) 568
BseDI CCNNGG 1 cut(s) 466
BseGI GGATG 1 cut(s) 190
BseLI CCNNNNNNNGG 2 cut(s) 247, 472
BseMII CTCAG 2 cut(s) 537, 782
BseRI GAGGAG 3 cut(s) 335, 563, 641
BseXI GCAGC 1 cut(s) 388
BshFI GGCC 1 cut(s) 727
BsiHKCI CYCGRG 1 cut(s) 153
BsiSI CCGG 2 cut(s) 98, 533
BslI CCNNNNNNNGG 2 cut(s) 247, 472
BsmAI GTCTC 1 cut(s) 650
BsmBI CGTCTC 1 cut(s) 650
BsmI GAATGC 1 cut(s) 420
BsnI GGCC 1 cut(s) 727
BsoBI CYCGRG 1 cut(s) 153
Bsp119I TTCGAA 2 cut(s) 412, 457
Bsp13I TCCGGA 1 cut(s) 97
Bsp143I GATC 1 cut(s) 462
BspACI CCGC 2 cut(s) 233, 276
BspANI GGCC 1 cut(s) 727
BspCNI CTCAG 2 cut(s) 538, 781
BspEI TCCGGA 1 cut(s) 97
BspLI GGNNCC 1 cut(s) 531
BspMI ACCTGC 1 cut(s) 565
BspOI GCTAGC 1 cut(s) 545
BspPI GGATC 1 cut(s) 457
BspQI GCTCTTC 1 cut(s) 84
BspT104I TTCGAA 2 cut(s) 412, 457
BssECI CCNNGG 1 cut(s) 466
BssMI GATC 1 cut(s) 462
BssNI GRCGYC 1 cut(s) 426
BssT1I CCWWGG 1 cut(s) 466
Bst2UI CCWGG 1 cut(s) 568
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 7 cut(s) 3, 18, 57, 84, 357, 591, 618
BstACI GRCGYC 1 cut(s) 426
BstBI TTCGAA 2 cut(s) 412, 457
BstC8I GCNNGC 4 cut(s) 136, 430, 543, 799
BstDEI CTNAG 2 cut(s) 546, 768
BstF5I GGATG 1 cut(s) 190
BstHHI GCGC 2 cut(s) 140, 434
BstKTI GATC 1 cut(s) 465
BstMAI GTCTC 1 cut(s) 650
BstMBI GATC 1 cut(s) 462
BstMWI GCNNNNNNNGC 2 cut(s) 429, 542
BstNI CCWGG 1 cut(s) 568
BstNSI RCATGY 1 cut(s) 801
BstSCI CCNGG 2 cut(s) 531, 566
BstSFI CTRYAG 1 cut(s) 443
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 681
BstX2I RGATCY 1 cut(s) 462
BstYI RGATCY 1 cut(s) 462
BsuI GTATCC 1 cut(s) 768
BsuRI GGCC 1 cut(s) 727
BtgZI GCGATG 1 cut(s) 795
BtsCI GGATG 1 cut(s) 190
BtsI GCAGTG 1 cut(s) 418
BtsIMutI CAGTG 2 cut(s) 418, 486
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 4 cut(s) 136, 430, 543, 799
CfoI GCGC 2 cut(s) 140, 434
Cfr13I GGNCC 3 cut(s) 289, 334, 529
CseI GACGC 1 cut(s) 434
Csp6I GTAC 2 cut(s) 107, 736
CspCI CAANNNNNGTGG 2 cut(s) 195, 230
CviAII CATG 1 cut(s) 798
CviQI GTAC 2 cut(s) 107, 736
DdeI CTNAG 2 cut(s) 546, 768
DpnI GATC 1 cut(s) 464
DpnII GATC 1 cut(s) 462
EaeI YGGCCR 1 cut(s) 725
Eam1104I CTCTTC 7 cut(s) 3, 18, 57, 84, 357, 591, 618
EarI CTCTTC 7 cut(s) 3, 18, 57, 84, 357, 591, 618
Eco130I CCWWGG 1 cut(s) 466
Eco32I GATATC 1 cut(s) 706
Eco47I GGWCC 3 cut(s) 289, 334, 529
Eco88I CYCGRG 1 cut(s) 153
EcoRI GAATTC 1 cut(s) 453
EcoRII CCWGG 1 cut(s) 566
EcoRV GATATC 1 cut(s) 706
EcoT14I CCWWGG 1 cut(s) 466
EcoT22I ATGCAT 2 cut(s) 126, 799
ErhI CCWWGG 1 cut(s) 466
Esp3I CGTCTC 1 cut(s) 650
FaeI CATG 1 cut(s) 801
FaiI YATR 5 cut(s) 122, 245, 558, 799, 809
FatI CATG 1 cut(s) 797
Fnu4HI GCNGC 3 cut(s) 234, 277, 377
FokI GGATG 1 cut(s) 197
Fsp4HI GCNGC 3 cut(s) 234, 277, 377
FspBI CTAG 1 cut(s) 542
FspI TGCGCA 1 cut(s) 139
GlaI GCGC 2 cut(s) 139, 433
GluI GCNGC 3 cut(s) 234, 277, 377
HaeIII GGCC 1 cut(s) 727
HapII CCGG 2 cut(s) 98, 533
HgaI GACGC 1 cut(s) 434
HhaI GCGC 2 cut(s) 140, 434
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 1 cut(s) 801
Hin6I GCGC 2 cut(s) 138, 432
HinP1I GCGC 2 cut(s) 138, 432
HindIII AAGCTT 1 cut(s) 132
HinfI GANTC 1 cut(s) 476
HpaII CCGG 2 cut(s) 98, 533
HphI GGTGA 2 cut(s) 232, 291
Hpy166II GTNNAC 2 cut(s) 289, 529
Hpy188I TCNGA 2 cut(s) 745, 771
Hpy188III TCNNGA 1 cut(s) 98
Hpy8I GTNNAC 2 cut(s) 289, 529
Hpy99I CGWCG 1 cut(s) 428
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 4 cut(s) 124, 406, 418, 797
HpyF10VI GCNNNNNNNGC 2 cut(s) 429, 542
HpyF3I CTNAG 2 cut(s) 546, 768
HpySE526I ACGT 1 cut(s) 492
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 1 cut(s) 801
HspAI GCGC 2 cut(s) 138, 432
Kpn2I TCCGGA 1 cut(s) 97
Kzo9I GATC 1 cut(s) 462
LguI GCTCTTC 1 cut(s) 84
Lsp1109I GCAGC 1 cut(s) 388
MaeI CTAG 1 cut(s) 542
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 187, 391
MalI GATC 1 cut(s) 464
MboI GATC 1 cut(s) 462
MflI RGATCY 1 cut(s) 462
MluCI AATT 1 cut(s) 453
Mph1103I ATGCAT 2 cut(s) 126, 799
MroI TCCGGA 1 cut(s) 97
MseI TTAA 1 cut(s) 345
MspI CCGG 2 cut(s) 98, 533
MspR9I CCNGG 2 cut(s) 533, 568
Mva1269I GAATGC 1 cut(s) 420
MvaI CCWGG 1 cut(s) 568
MwoI GCNNNNNNNGC 2 cut(s) 429, 542
NciI CCSGG 1 cut(s) 533
NdeII GATC 1 cut(s) 462
NheI GCTAGC 1 cut(s) 541
NlaIII CATG 1 cut(s) 801
NlaIV GGNNCC 1 cut(s) 531
NmeAIII GCCGAG 1 cut(s) 667
NsbI TGCGCA 1 cut(s) 139
NsiI ATGCAT 2 cut(s) 126, 799
NspI RCATGY 1 cut(s) 801
NspV TTCGAA 2 cut(s) 412, 457
PaeI GCATGC 1 cut(s) 801
PaeR7I CTCGAG 1 cut(s) 153
PciSI GCTCTTC 1 cut(s) 84
PctI GAATGC 1 cut(s) 420
PfeI GAWTC 1 cut(s) 476
PflMI CCANNNNNTGG 1 cut(s) 472
PkrI GCNGC 3 cut(s) 235, 278, 378
Psp6I CCWGG 1 cut(s) 566
PspGI CCWGG 1 cut(s) 566
PspN4I GGNNCC 1 cut(s) 531
PspPI GGNCC 3 cut(s) 289, 334, 529
PspXI VCTCGAGB 1 cut(s) 153
PsrI GAACNNNNNNTAC 2 cut(s) 393, 425
PsuI RGATCY 1 cut(s) 462
RsaI GTAC 2 cut(s) 108, 737
RsaNI GTAC 2 cut(s) 107, 736
SapI GCTCTTC 1 cut(s) 84
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 3 cut(s) 234, 277, 377
Sau3AI GATC 1 cut(s) 462
Sau96I GGNCC 3 cut(s) 289, 334, 529
ScaI AGTACT 1 cut(s) 737
ScrFI CCNGG 2 cut(s) 533, 568
SfcI CTRYAG 1 cut(s) 443
Sfr274I CTCGAG 1 cut(s) 153
SfuI TTCGAA 2 cut(s) 412, 457
SinI GGWCC 3 cut(s) 289, 334, 529
SlaI CTCGAG 1 cut(s) 153
SmlI CTYRAG 3 cut(s) 110, 153, 435
SmoI CTYRAG 3 cut(s) 110, 153, 435
SphI GCATGC 1 cut(s) 801
Sse9I AATT 1 cut(s) 453
SsiI CCGC 2 cut(s) 233, 276
SspI AATATT 1 cut(s) 722
SspMI CTAG 1 cut(s) 542
StyD4I CCNGG 2 cut(s) 531, 566
StyI CCWWGG 1 cut(s) 466
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 495
TaqI TCGA 4 cut(s) 154, 412, 457, 702
TasI AATT 1 cut(s) 453
TatI WGTACW 2 cut(s) 106, 735
TauI GCSGC 2 cut(s) 236, 279
TfiI GAWTC 1 cut(s) 476
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 425, 486
TseI GCWGC 1 cut(s) 376
TspDTI ATGAA 3 cut(s) 63, 741, 798
TspRI CASTG 2 cut(s) 425, 486
Van91I CCANNNNNTGG 1 cut(s) 472
VpaK11BI GGWCC 3 cut(s) 289, 334, 529
XapI RAATTY 1 cut(s) 453
XceI RCATGY 1 cut(s) 801
XhoI CTCGAG 1 cut(s) 153
XspI CTAG 1 cut(s) 542
ZrmI AGTACT 1 cut(s) 737
Zsp2I ATGCAT 2 cut(s) 126, 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.