pycom14g12820

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0002139
Physical Location & Seq
Reverse (-)
15869355 .. 15871191
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g12820.3

Sequence Viewer

Length: 1032 bp
ATGGGGGAGGAGGAGAAGAAAATGGAGACGAAGCCAGAGGCGGAGGAGAAGAAGGCCGGAGAGAAAAAGCCTGAAGCTGCCGAGCAAAAGAAGGAGAAAGCCGAGGAGGGAAAAGATGGGAAAGCAGAGGCCAAGGAGGGTGATGCCGCTACCGCTCCTCCGCCTCCGCCTCCACCCCAAGAAATTGTGCTCAAAGTTTATATGCATTGTGAAGGGTGTGCTCGCAAGGTCAAGCGGTGCCTCAAAGGATTTCAAGGGGTGGAAGATGTGAGCACAGATTGCAAGACTCACAAGGTGGTGGTGAAGGGAGAGAAGGCTGAGCCAATTAAGGTTCTTGAAAGAGTTCAGAGGAAGAGCCACCGGCAGGTGGAGCTTCTCTCTCCAATCCCAAAACCGCCGGCGGAGGAGGAGAAGAAGGCGGAGGAGAAAGAGAAGCCCAAACCAGAGGAAAAGAAAGAAGAGCCTCAGGTAATCACAGTAGTGCTGAAAGTTCACATGCATTGCGACGCCTGTGCCCAAGAAATCAAGAAACGCATTCTCAGAATGAAAGGAGTGGAATCTGCTGAGGCGGATCTTACGAGCTCAGAGGTGACAGTGAAGGGTGTGTTTGATCCGCCTAAGCTAGTTGATTATGTGCACAAAAGAACCGGGAGGCACGCGGCGATCGTGAAGCAAGAACCGGAGAAGAAAGAGAAAGAAGAAGCCGAAGAAGCCAAAGATGAGAAGAAAGGTGGTAAAGGTGACAAAGATAAGAAGGGCAAAGATGGTGGTGGCGATGAAGCGAAAGCAGAAAAGAACAAGGAAAATAAAGGTGACAACAATGAAGCTGCCGCCGCGGCCGCTGGTGGTGCACCGGAAGGAGCTGCCGCAGAAGAGACCAAGGTGGTGGAGCTGATGAAGAAGAATGAATACTATTCTTACCCACCAAGGTATGCCATGGAACAGTATGCCTACCCACCTCAGATCATGGAAAATCCCTACCCACCTCAGATCTTCAGTGATGAGAACCCTAATGCCTGCTCTATCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

344

Amino Acids

38.03

Weight (kDa)

6.14

Isoelectric Point (pI)

53.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 355
Acc36I ACCTGC 1 cut(s) 355
AccB1I GGYRCC 1 cut(s) 237
AccBSI CCGCTC 1 cut(s) 155
AccII CGCG 2 cut(s) 659, 836
AclWI GGATC 2 cut(s) 579, 605
AcoI YGGCCR 1 cut(s) 837
AcuI CTGAAG 2 cut(s) 93, 979
AcyI GRCGYC 1 cut(s) 507
AdeI CACNNNGTG 1 cut(s) 295
AfiI CCNNNNNNNGG 2 cut(s) 364, 367
AgsI TTSAA 2 cut(s) 254, 338
AleI CACNNNNGTG 1 cut(s) 479
AluBI AGCT 7 cut(s) 77, 373, 582, 622, 827, 863, 892
AluI AGCT 7 cut(s) 77, 373, 582, 622, 827, 863, 892
Alw21I GWGCWC 6 cut(s) 192, 223, 275, 584, 639, 853
Alw26I GTCTC 2 cut(s) 20, 869
Alw44I GTGCAC 2 cut(s) 635, 849
AlwI GGATC 2 cut(s) 579, 605
AoxI GGCC 3 cut(s) 54, 129, 837
ApaLI GTGCAC 2 cut(s) 635, 849
ApeKI GCWGC 3 cut(s) 77, 827, 863
Asp700I GAANNNNTTC 1 cut(s) 342
AsuC2I CCSGG 1 cut(s) 649
AsuHPI GGTGA 5 cut(s) 152, 313, 601, 752, 824
AxyI CCTNAGG 1 cut(s) 465
BaeGI GKGCMC 3 cut(s) 517, 639, 853
BanI GGYRCC 1 cut(s) 237
BanII GRGCYC 1 cut(s) 584
BarI GAAGNNNNNNTAC 2 cut(s) 893, 925
Bbv12I GWGCWC 6 cut(s) 192, 223, 275, 584, 639, 853
BbvCI CCTCAGC 1 cut(s) 564
BbvI GCAGC 3 cut(s) 64, 814, 850
BccI CCATC 2 cut(s) 110, 758
BcgI CGANNNNNNTGC 2 cut(s) 494, 528
BcnI CCSGG 1 cut(s) 649
BcoDI GTCTC 2 cut(s) 20, 869
BfaI CTAG 1 cut(s) 623
BfuAI ACCTGC 1 cut(s) 355
BglI GCCNNNNNGGC 1 cut(s) 836
BglII AGATCT 1 cut(s) 990
BlpI GCTNAGC 1 cut(s) 318
Bme1390I CCNGG 1 cut(s) 649
BmiI GGNNCC 1 cut(s) 239
BmrFI CCNGG 1 cut(s) 649
BmsI GCATC 1 cut(s) 133
BplI GAGNNNNNCTC 2 cut(s) 362, 394
Bpu10I CCTNAGC 2 cut(s) 564, 618
Bpu1102I GCTNAGC 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 649
BsaHI GRCGYC 1 cut(s) 507
BsaI GGTCTC 1 cut(s) 869
BsaJI CCNNGG 6 cut(s) 102, 132, 834, 879, 926, 936
BsaWI WCCGGW 2 cut(s) 679, 853
BsaXI ACNNNNNCTCC 2 cut(s) 142, 172
Bsc4I CCNNNNNNNGG 2 cut(s) 364, 367
Bse118I RCCGGY 2 cut(s) 360, 397
Bse21I CCTNAGG 1 cut(s) 465
Bse3DI GCAATG 1 cut(s) 499
BseDI CCNNGG 6 cut(s) 102, 132, 834, 879, 926, 936
BseLI CCNNNNNNNGG 2 cut(s) 364, 367
BseMI GCAATG 1 cut(s) 499
BseMII CTCAG 7 cut(s) 309, 479, 553, 555, 597, 974, 1001
BseRI GAGGAG 8 cut(s) 23, 26, 59, 119, 147, 419, 422, 437
BseSI GKGCMC 3 cut(s) 517, 639, 853
BseX3I CGGCCG 1 cut(s) 837
BseXI GCAGC 3 cut(s) 64, 814, 850
Bsh1236I CGCG 2 cut(s) 659, 836
Bsh1285I CGRYCG 2 cut(s) 666, 840
BshFI GGCC 3 cut(s) 56, 131, 839
BshNI GGYRCC 1 cut(s) 237
BsiEI CGRYCG 2 cut(s) 666, 840
BsiHKAI GWGCWC 6 cut(s) 192, 223, 275, 584, 639, 853
BsiSI CCGG 6 cut(s) 57, 361, 398, 648, 680, 854
BslI CCNNNNNNNGG 2 cut(s) 364, 367
BsmAI GTCTC 2 cut(s) 20, 869
BsmBI CGTCTC 1 cut(s) 20
BsmI GAATGC 1 cut(s) 534
BsnI GGCC 3 cut(s) 56, 131, 839
Bso31I GGTCTC 1 cut(s) 869
Bsp1286I GDGCHC 7 cut(s) 192, 223, 275, 517, 584, 639, 853
Bsp143I GATC 5 cut(s) 571, 610, 663, 963, 990
Bsp1720I GCTNAGC 1 cut(s) 318
Bsp19I CCATGG 1 cut(s) 936
BspANI GGCC 3 cut(s) 56, 131, 839
BspCNI CTCAG 7 cut(s) 310, 478, 552, 556, 596, 973, 1000
BspFNI CGCG 2 cut(s) 659, 836
BspLI GGNNCC 1 cut(s) 239
BspMI ACCTGC 1 cut(s) 355
BspPI GGATC 2 cut(s) 579, 605
BspQI GCTCTTC 2 cut(s) 347, 453
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 869
BsrBI CCGCTC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 499
BsrFI RCCGGY 2 cut(s) 360, 397
BssAI RCCGGY 2 cut(s) 360, 397
BssECI CCNNGG 6 cut(s) 102, 132, 834, 879, 926, 936
BssMI GATC 5 cut(s) 571, 610, 663, 963, 990
BssNI GRCGYC 1 cut(s) 507
BssT1I CCWWGG 4 cut(s) 132, 879, 926, 936
Bst4CI ACNGT 3 cut(s) 478, 595, 945
Bst6I CTCTTC 3 cut(s) 347, 453, 867
BstACI GRCGYC 1 cut(s) 507
BstAPI GCANNNNNTGC 1 cut(s) 279
BstC8I GCNNGC 4 cut(s) 223, 399, 657, 1018
BstDEI CTNAG 8 cut(s) 318, 465, 539, 564, 583, 618, 960, 987
BstDSI CCRYGG 2 cut(s) 834, 936
BstFNI CGCG 2 cut(s) 659, 836
BstKTI GATC 5 cut(s) 574, 613, 666, 966, 993
BstMAI GTCTC 2 cut(s) 20, 869
BstMBI GATC 5 cut(s) 571, 610, 663, 963, 990
BstMCI CGRYCG 2 cut(s) 666, 840
BstMWI GCNNNNNNNGC 8 cut(s) 152, 279, 370, 710, 833, 836, 839, 848
BstNSI RCATGY 1 cut(s) 499
BstSCI CCNGG 1 cut(s) 647
BstSLI GKGCMC 3 cut(s) 517, 639, 853
BstUI CGCG 2 cut(s) 659, 836
BstV1I GCAGC 3 cut(s) 64, 814, 850
BstX2I RGATCY 2 cut(s) 571, 990
BstXI CCANNNNNNTGG 1 cut(s) 886
BstYI RGATCY 2 cut(s) 571, 990
BstZI CGGCCG 1 cut(s) 837
Bsu36I CCTNAGG 1 cut(s) 465
BsuRI GGCC 3 cut(s) 56, 131, 839
BtgI CCRYGG 2 cut(s) 834, 936
BtgZI GCGATG 1 cut(s) 789
BtsIMutI CAGTG 2 cut(s) 600, 1003
BveI ACCTGC 1 cut(s) 355
Cac8I GCNNGC 4 cut(s) 223, 399, 657, 1018
CciNI GCGGCCGC 1 cut(s) 837
Cfr10I RCCGGY 2 cut(s) 360, 397
Cfr42I CCGCGG 1 cut(s) 837
CseI GACGC 1 cut(s) 515
CspCI CAANNNNNGTGG 2 cut(s) 748, 783
CviAII CATG 4 cut(s) 496, 937, 967, 1027
DdeI CTNAG 8 cut(s) 318, 465, 539, 564, 583, 618, 960, 987
DpnI GATC 5 cut(s) 573, 612, 665, 965, 992
DpnII GATC 5 cut(s) 571, 610, 663, 963, 990
DraIII CACNNNGTG 1 cut(s) 295
EaeI YGGCCR 1 cut(s) 837
EagI CGGCCG 1 cut(s) 837
Eam1104I CTCTTC 3 cut(s) 347, 453, 867
EarI CTCTTC 3 cut(s) 347, 453, 867
EciI GGCGGA 7 cut(s) 56, 150, 156, 416, 434, 584, 603
Ecl136II GAGCTC 1 cut(s) 582
EclXI CGGCCG 1 cut(s) 837
Eco130I CCWWGG 4 cut(s) 132, 879, 926, 936
Eco24I GRGCYC 1 cut(s) 584
Eco31I GGTCTC 1 cut(s) 869
Eco52I CGGCCG 1 cut(s) 837
Eco53kI GAGCTC 1 cut(s) 582
Eco57I CTGAAG 2 cut(s) 93, 979
Eco81I CCTNAGG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 582
EcoT14I CCWWGG 4 cut(s) 132, 879, 926, 936
EcoT22I ATGCAT 2 cut(s) 207, 501
EcoT38I GRGCYC 1 cut(s) 584
ErhI CCWWGG 4 cut(s) 132, 879, 926, 936
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 4 cut(s) 499, 940, 970, 1030
FaiI YATR 9 cut(s) 201, 203, 497, 633, 933, 938, 948, 968, 1028
FatI CATG 4 cut(s) 495, 936, 966, 1026
FriOI GRGCYC 1 cut(s) 584
FspBI CTAG 1 cut(s) 623
HaeIII GGCC 3 cut(s) 56, 131, 839
HapII CCGG 6 cut(s) 57, 361, 398, 648, 680, 854
HgaI GACGC 1 cut(s) 515
Hin1I GRCGYC 1 cut(s) 507
Hin1II CATG 4 cut(s) 499, 940, 970, 1030
HinfI GANTC 2 cut(s) 286, 557
HpaII CCGG 6 cut(s) 57, 361, 398, 648, 680, 854
HphI GGTGA 5 cut(s) 152, 313, 601, 752, 824
Hpy166II GTNNAC 3 cut(s) 493, 637, 851
Hpy188I TCNGA 5 cut(s) 348, 542, 586, 963, 990
Hpy188III TCNNGA 3 cut(s) 335, 526, 667
Hpy8I GTNNAC 3 cut(s) 493, 637, 851
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 9 cut(s) 46, 85, 206, 298, 307, 409, 592, 748, 851
HpyCH4III ACNGT 3 cut(s) 478, 595, 945
HpyCH4V TGCA 5 cut(s) 205, 282, 499, 637, 851
HpyF10VI GCNNNNNNNGC 8 cut(s) 152, 279, 370, 710, 833, 836, 839, 848
HpyF3I CTNAG 8 cut(s) 318, 465, 539, 564, 583, 618, 960, 987
Hsp92I GRCGYC 1 cut(s) 507
Hsp92II CATG 4 cut(s) 499, 940, 970, 1030
KroI GCCGGC 1 cut(s) 397
KroNI GCCGGC 1 cut(s) 399
KspI CCGCGG 1 cut(s) 837
Kzo9I GATC 5 cut(s) 571, 610, 663, 963, 990
LguI GCTCTTC 2 cut(s) 347, 453
LmnI GCTCC 4 cut(s) 160, 370, 860, 889
Lsp1109I GCAGC 3 cut(s) 64, 814, 850
LweI GCATC 1 cut(s) 133
MaeI CTAG 1 cut(s) 623
MaeIII GTNAC 3 cut(s) 589, 740, 812
MalI GATC 5 cut(s) 573, 612, 665, 965, 992
MbiI CCGCTC 1 cut(s) 155
MboI GATC 5 cut(s) 571, 610, 663, 963, 990
MflI RGATCY 2 cut(s) 571, 990
MhlI GDGCHC 7 cut(s) 192, 223, 275, 517, 584, 639, 853
MluCI AATT 2 cut(s) 183, 324
MlyI GAGTC 1 cut(s) 280
Mph1103I ATGCAT 2 cut(s) 207, 501
MreI CGCCGGCG 1 cut(s) 397
MroNI GCCGGC 1 cut(s) 397
MroXI GAANNNNTTC 1 cut(s) 342
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 479
MspA1I CMGCKG 2 cut(s) 836, 842
MspI CCGG 6 cut(s) 57, 361, 398, 648, 680, 854
MspR9I CCNGG 1 cut(s) 649
Mva1269I GAATGC 1 cut(s) 534
MvnI CGCG 2 cut(s) 659, 836
MwoI GCNNNNNNNGC 8 cut(s) 152, 279, 370, 710, 833, 836, 839, 848
NaeI GCCGGC 1 cut(s) 399
NciI CCSGG 1 cut(s) 649
NcoI CCATGG 1 cut(s) 936
NdeII GATC 5 cut(s) 571, 610, 663, 963, 990
NgoMIV GCCGGC 1 cut(s) 397
NlaIII CATG 4 cut(s) 499, 940, 970, 1030
NlaIV GGNNCC 1 cut(s) 239
NmeAIII GCCGAG 2 cut(s) 106, 127
NmuCI GTSAC 3 cut(s) 589, 740, 812
NotI GCGGCCGC 1 cut(s) 837
NsiI ATGCAT 2 cut(s) 207, 501
NspI RCATGY 1 cut(s) 499
OliI CACNNNNGTG 1 cut(s) 479
PaqCI CACCTGC 1 cut(s) 355
PciSI GCTCTTC 2 cut(s) 347, 453
PcsI WCGNNNNNNNCGW 1 cut(s) 663
PctI GAATGC 1 cut(s) 534
PdiI GCCGGC 1 cut(s) 399
PdmI GAANNNNTTC 1 cut(s) 342
PfeI GAWTC 1 cut(s) 557
Ple19I CGATCG 1 cut(s) 666
PleI GAGTC 1 cut(s) 280
PpsI GAGTC 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 584
PspN4I GGNNCC 1 cut(s) 239
PsuI RGATCY 2 cut(s) 571, 990
PvuI CGATCG 1 cut(s) 666
RseI CAYNNNNRTG 1 cut(s) 479
SacI GAGCTC 1 cut(s) 584
SacII CCGCGG 1 cut(s) 837
SapI GCTCTTC 2 cut(s) 347, 453
SaqAI TTAA 1 cut(s) 327
Sau3AI GATC 5 cut(s) 571, 610, 663, 963, 990
SchI GAGTC 1 cut(s) 280
ScrFI CCNGG 1 cut(s) 649
SduI GDGCHC 7 cut(s) 192, 223, 275, 517, 584, 639, 853
SfaNI GCATC 1 cut(s) 133
Sfr303I CCGCGG 1 cut(s) 837
SgrAI CRCCGGYG 1 cut(s) 397
SgrBI CCGCGG 1 cut(s) 837
SmiMI CAYNNNNRTG 1 cut(s) 479
Sse9I AATT 2 cut(s) 183, 324
SspMI CTAG 1 cut(s) 623
SstI GAGCTC 1 cut(s) 584
StyD4I CCNGG 1 cut(s) 647
StyI CCWWGG 4 cut(s) 132, 879, 926, 936
TaaI ACNGT 3 cut(s) 478, 595, 945
TasI AATT 2 cut(s) 183, 324
TauI GCSGC 7 cut(s) 149, 662, 833, 836, 839, 842, 869
TfiI GAWTC 1 cut(s) 557
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 2 cut(s) 600, 1003
TseFI GTSAC 3 cut(s) 589, 740, 812
TseI GCWGC 3 cut(s) 77, 827, 863
Tsp45I GTSAC 3 cut(s) 589, 740, 812
TspDTI ATGAA 5 cut(s) 560, 792, 837, 911, 921
TspRI CASTG 2 cut(s) 600, 1003
VneI GTGCAC 2 cut(s) 635, 849
XceI RCATGY 1 cut(s) 499
XmnI GAANNNNTTC 1 cut(s) 342
XspI CTAG 1 cut(s) 623
Zsp2I ATGCAT 2 cut(s) 207, 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.