pycom10g28780

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0002139
Physical Location & Seq
Forward (+)
29287143 .. 29292358
5216 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g28780.5

Sequence Viewer

Length: 795 bp
ATGGGCGAAGAGAAGAAAGAGGAAGAGAAGAAGGACGAAAGCAAAGATGAAAAGAAAGAAGAAGAGAAAAAGGAAGAAAAGAAAGAAGAGCCTCCGGAGATTGTACTCAAGGTCGATATGCATTGTGAAGCTTGCGCAAGGAAAGTTGCTCGAGCCTTGAAGGGTTTTGAAGGAGTGGAGGATGTAACGACAGACAGCAAGGCGAGTAAGGTGGTGGTGAAAGGGAAGGCGGCAGACCCCATAAAGGTCTGCGAAAGATTACAACAGAAGAGCGGCAAAAAAGTGGAGCTCATTTCACCTTTGCCTAAACCTCCCGAGGAGAAGGAGGAAGAACAAGTTAAGGAAGCCGACAAGGAAGCGAAAAAAGAGGAGCCTCCTGCTGTTGTTTCAGTGGTATTGCAAGTTCGGATGCACTGCGATGCGTGCGCCCAAGGTCTACAGAAGCGAATTCGAAAGATCCAAGGTGTGGAGTCAGTGGAAACAGACGTAGGCAACAACAAGGTTGTAGTAAAAGGCGTGATGGACCCGGCGAAGCTTGCGGAGGATGTGTATAAAAAAACCAGGAAGCAGGTTTCTGTTGTGAAGGAGGATGAGAAAAAAGAAGAAAAGAAAGAAGACGAGAACAAGAAGGAAGGAGAAAAGAAAGAAGGGGAGGAAGACAAGCAGGGAGAGGACAATAAGGTCGATATAAAGCGAAGCGATTATTGGCCAACAAAGTACTACTCAGAATATTCATCGTATCCCCACCCTCAGATTTTCAGCGATGAAAACCCTAATGCATGCTCTGTTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

265

Amino Acids

30.08

Weight (kDa)

5.46

Isoelectric Point (pI)

55.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 680
Acc16I TGCGCA 1 cut(s) 136
Acc36I ACCTGC 1 cut(s) 559
AccB7I CCANNNNNTGG 1 cut(s) 466
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 1 cut(s) 436
AccIII TCCGGA 1 cut(s) 94
AciI CCGC 3 cut(s) 230, 273, 539
AclWI GGATC 1 cut(s) 451
AcoI YGGCCR 1 cut(s) 707
AcsI RAATTY 1 cut(s) 447
AfaI GTAC 2 cut(s) 105, 719
AfiI CCNNNNNNNGG 2 cut(s) 244, 466
AgsI TTSAA 2 cut(s) 160, 170
AjnI CCWGG 1 cut(s) 560
AjuI GAANNNNNNNTTGG 2 cut(s) 688, 720
AluBI AGCT 3 cut(s) 131, 289, 535
AluI AGCT 3 cut(s) 131, 289, 535
Alw21I GWGCWC 1 cut(s) 291
AlwI GGATC 1 cut(s) 451
Ama87I CYCGRG 2 cut(s) 150, 314
Aor13HI TCCGGA 1 cut(s) 94
AoxI GGCC 1 cut(s) 707
ApoI RAATTY 1 cut(s) 447
AspLEI GCGC 2 cut(s) 137, 428
AspS9I GGNCC 1 cut(s) 523
AsuC2I CCSGG 1 cut(s) 527
AsuHPI GGTGA 2 cut(s) 229, 288
AsuII TTCGAA 1 cut(s) 451
AvaI CYCGRG 2 cut(s) 150, 314
AvaII GGWCC 1 cut(s) 523
BalI TGGCCA 1 cut(s) 709
BanII GRGCYC 1 cut(s) 291
BbsI GAAGAC 2 cut(s) 621, 663
Bbv12I GWGCWC 1 cut(s) 291
BccI CCATC 1 cut(s) 514
BciT130I CCWGG 1 cut(s) 562
BciVI GTATCC 1 cut(s) 750
BcnI CCSGG 1 cut(s) 527
BfmI CTRYAG 1 cut(s) 437
BfuAI ACCTGC 1 cut(s) 559
BfuI GTATCC 1 cut(s) 750
BisI GCNGC 2 cut(s) 231, 274
BlsI GCNGC 2 cut(s) 232, 275
BmcAI AGTACT 1 cut(s) 719
Bme1390I CCNGG 2 cut(s) 527, 562
Bme18I GGWCC 1 cut(s) 523
BmeT110I CYCGRG 2 cut(s) 150, 314
BmgT120I GGNCC 1 cut(s) 523
BmiI GGNNCC 2 cut(s) 372, 525
BmrFI CCNGG 2 cut(s) 527, 562
BmsI GCATC 2 cut(s) 399, 409
BpiI GAAGAC 2 cut(s) 621, 663
Bpu14I TTCGAA 1 cut(s) 451
BpuEI CTTGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 527
BsaJI CCNNGG 3 cut(s) 315, 430, 460
BsaWI WCCGGW 1 cut(s) 94
BsaXI ACNNNNNCTCC 2 cut(s) 533, 563
Bsc4I CCNNNNNNNGG 2 cut(s) 244, 466
BseAI TCCGGA 1 cut(s) 94
BseBI CCWGG 1 cut(s) 562
BseDI CCNNGG 3 cut(s) 315, 430, 460
BseGI GGATG 4 cut(s) 187, 414, 550, 595
BseLI CCNNNNNNNGG 2 cut(s) 244, 466
BseMII CTCAG 2 cut(s) 738, 764
BseRI GAGGAG 2 cut(s) 332, 383
BshFI GGCC 1 cut(s) 709
BsiHKAI GWGCWC 1 cut(s) 291
BsiHKCI CYCGRG 2 cut(s) 150, 314
BsiSI CCGG 2 cut(s) 95, 527
BslI CCNNNNNNNGG 2 cut(s) 244, 466
BsnI GGCC 1 cut(s) 709
BsoBI CYCGRG 2 cut(s) 150, 314
Bsp119I TTCGAA 1 cut(s) 451
Bsp1286I GDGCHC 1 cut(s) 291
Bsp13I TCCGGA 1 cut(s) 94
Bsp143I GATC 1 cut(s) 456
BspACI CCGC 3 cut(s) 230, 273, 539
BspANI GGCC 1 cut(s) 709
BspCNI CTCAG 2 cut(s) 737, 763
BspEI TCCGGA 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 372, 525
BspMI ACCTGC 1 cut(s) 559
BspPI GGATC 1 cut(s) 451
BspQI GCTCTTC 2 cut(s) 81, 263
BspT104I TTCGAA 1 cut(s) 451
BsrBI CCGCTC 1 cut(s) 273
BssECI CCNNGG 3 cut(s) 315, 430, 460
BssMI GATC 1 cut(s) 456
BssT1I CCWWGG 2 cut(s) 430, 460
Bst2UI CCWGG 1 cut(s) 562
Bst6I CTCTTC 5 cut(s) 3, 18, 57, 81, 263
BstBI TTCGAA 1 cut(s) 451
BstC8I GCNNGC 4 cut(s) 133, 424, 537, 781
BstDEI CTNAG 2 cut(s) 724, 750
BstF5I GGATG 4 cut(s) 187, 414, 550, 595
BstHHI GCGC 2 cut(s) 137, 428
BstKTI GATC 1 cut(s) 459
BstMBI GATC 1 cut(s) 456
BstMWI GCNNNNNNNGC 2 cut(s) 423, 536
BstNI CCWGG 1 cut(s) 562
BstNSI RCATGY 1 cut(s) 783
BstSCI CCNGG 2 cut(s) 525, 560
BstSFI CTRYAG 1 cut(s) 437
BstV2I GAAGAC 2 cut(s) 621, 663
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
BsuI GTATCC 1 cut(s) 750
BsuRI GGCC 1 cut(s) 709
BtgZI GCGATG 2 cut(s) 432, 777
BtsCI GGATG 4 cut(s) 187, 414, 550, 595
BtsI GCAGTG 1 cut(s) 412
BtsIMutI CAGTG 3 cut(s) 396, 412, 480
BveI ACCTGC 1 cut(s) 559
Cac8I GCNNGC 4 cut(s) 133, 424, 537, 781
CfoI GCGC 2 cut(s) 137, 428
Cfr13I GGNCC 1 cut(s) 523
Csp6I GTAC 2 cut(s) 104, 718
CviAII CATG 1 cut(s) 780
CviJI RGCY 8 cut(s) 91, 131, 155, 289, 347, 373, 535, 709
CviKI_1 RGCY 8 cut(s) 91, 131, 155, 289, 347, 373, 535, 709
CviQI GTAC 2 cut(s) 104, 718
DdeI CTNAG 2 cut(s) 724, 750
DpnI GATC 1 cut(s) 458
DpnII GATC 1 cut(s) 456
DrdI GACNNNNNNGTC 1 cut(s) 680
DseDI GACNNNNNNGTC 1 cut(s) 680
EaeI YGGCCR 1 cut(s) 707
Eam1104I CTCTTC 5 cut(s) 3, 18, 57, 81, 263
EarI CTCTTC 5 cut(s) 3, 18, 57, 81, 263
Ecl136II GAGCTC 1 cut(s) 289
Eco130I CCWWGG 2 cut(s) 430, 460
Eco24I GRGCYC 1 cut(s) 291
Eco47I GGWCC 1 cut(s) 523
Eco53kI GAGCTC 1 cut(s) 289
Eco88I CYCGRG 2 cut(s) 150, 314
EcoICRI GAGCTC 1 cut(s) 289
EcoRI GAATTC 1 cut(s) 447
EcoRII CCWGG 1 cut(s) 560
EcoT14I CCWWGG 2 cut(s) 430, 460
EcoT22I ATGCAT 2 cut(s) 123, 781
EcoT38I GRGCYC 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 430, 460
FaeI CATG 1 cut(s) 783
FaiI YATR 6 cut(s) 119, 242, 552, 689, 781, 791
FatI CATG 1 cut(s) 779
FblI GTMKAC 1 cut(s) 436
Fnu4HI GCNGC 2 cut(s) 231, 274
FokI GGATG 4 cut(s) 194, 421, 557, 602
FriOI GRGCYC 1 cut(s) 291
Fsp4HI GCNGC 2 cut(s) 231, 274
FspI TGCGCA 1 cut(s) 136
GlaI GCGC 2 cut(s) 136, 427
GluI GCNGC 2 cut(s) 231, 274
HaeIII GGCC 1 cut(s) 709
HapII CCGG 2 cut(s) 95, 527
HhaI GCGC 2 cut(s) 137, 428
Hin1II CATG 1 cut(s) 783
Hin6I GCGC 2 cut(s) 135, 426
HinP1I GCGC 2 cut(s) 135, 426
HindIII AAGCTT 2 cut(s) 129, 533
HinfI GANTC 1 cut(s) 470
HpaII CCGG 2 cut(s) 95, 527
HphI GGTGA 2 cut(s) 229, 288
Hpy166II GTNNAC 1 cut(s) 437
Hpy188I TCNGA 3 cut(s) 408, 727, 753
Hpy188III TCNNGA 2 cut(s) 95, 314
Hpy8I GTNNAC 1 cut(s) 437
HpyAV CCTTC 9 cut(s) 25, 154, 164, 220, 316, 577, 622, 626, 641
HpyCH4IV ACGT 1 cut(s) 486
HpyCH4V TGCA 4 cut(s) 121, 400, 412, 779
HpyF10VI GCNNNNNNNGC 2 cut(s) 423, 536
HpyF3I CTNAG 2 cut(s) 724, 750
HpySE526I ACGT 1 cut(s) 486
Hsp92II CATG 1 cut(s) 783
HspAI GCGC 2 cut(s) 135, 426
Kpn2I TCCGGA 1 cut(s) 94
Kzo9I GATC 1 cut(s) 456
LguI GCTCTTC 2 cut(s) 81, 263
LmnI GCTCC 2 cut(s) 286, 370
LpnPI CCDG 7 cut(s) 108, 390, 540, 547, 554, 574, 650
LweI GCATC 2 cut(s) 399, 409
MaeII ACGT 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 184
MalI GATC 1 cut(s) 458
MbiI CCGCTC 1 cut(s) 273
MboI GATC 1 cut(s) 456
MflI RGATCY 1 cut(s) 456
MhlI GDGCHC 1 cut(s) 291
MlsI TGGCCA 1 cut(s) 709
MluCI AATT 1 cut(s) 447
MluNI TGGCCA 1 cut(s) 709
MlyI GAGTC 1 cut(s) 479
Mox20I TGGCCA 1 cut(s) 709
Mph1103I ATGCAT 2 cut(s) 123, 781
MroI TCCGGA 1 cut(s) 94
MscI TGGCCA 1 cut(s) 709
MseI TTAA 1 cut(s) 339
MslI CAYNNNNRTG 1 cut(s) 417
Msp20I TGGCCA 1 cut(s) 709
MspI CCGG 2 cut(s) 95, 527
MspR9I CCNGG 2 cut(s) 527, 562
MvaI CCWGG 1 cut(s) 562
MwoI GCNNNNNNNGC 2 cut(s) 423, 536
NciI CCSGG 1 cut(s) 527
NdeII GATC 1 cut(s) 456
NlaIII CATG 1 cut(s) 783
NlaIV GGNNCC 2 cut(s) 372, 525
NsbI TGCGCA 1 cut(s) 136
NsiI ATGCAT 2 cut(s) 123, 781
NspI RCATGY 1 cut(s) 783
NspV TTCGAA 1 cut(s) 451
PaeI GCATGC 1 cut(s) 783
PaeR7I CTCGAG 1 cut(s) 150
PciSI GCTCTTC 2 cut(s) 81, 263
PflMI CCANNNNNTGG 1 cut(s) 466
PkrI GCNGC 2 cut(s) 232, 275
PleI GAGTC 1 cut(s) 478
PpsI GAGTC 1 cut(s) 478
Psp124BI GAGCTC 1 cut(s) 291
Psp6I CCWGG 1 cut(s) 560
PspGI CCWGG 1 cut(s) 560
PspN4I GGNNCC 2 cut(s) 372, 525
PspPI GGNCC 1 cut(s) 523
PspXI VCTCGAGB 1 cut(s) 150
PsrI GAACNNNNNNTAC 2 cut(s) 387, 419
PsuI RGATCY 1 cut(s) 456
RsaI GTAC 2 cut(s) 105, 719
RsaNI GTAC 2 cut(s) 104, 718
RseI CAYNNNNRTG 1 cut(s) 417
SacI GAGCTC 1 cut(s) 291
SapI GCTCTTC 2 cut(s) 81, 263
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 2 cut(s) 231, 274
Sau3AI GATC 1 cut(s) 456
Sau96I GGNCC 1 cut(s) 523
ScaI AGTACT 1 cut(s) 719
SchI GAGTC 1 cut(s) 479
ScrFI CCNGG 2 cut(s) 527, 562
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 2 cut(s) 399, 409
SfcI CTRYAG 1 cut(s) 437
Sfr274I CTCGAG 1 cut(s) 150
SfuI TTCGAA 1 cut(s) 451
SinI GGWCC 1 cut(s) 523
SlaI CTCGAG 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 417
SmlI CTYRAG 2 cut(s) 107, 150
SmoI CTYRAG 2 cut(s) 107, 150
SphI GCATGC 1 cut(s) 783
Sse9I AATT 1 cut(s) 447
SsiI CCGC 3 cut(s) 230, 273, 539
SspI AATATT 1 cut(s) 731
SstI GAGCTC 1 cut(s) 291
StyD4I CCNGG 2 cut(s) 525, 560
StyI CCWWGG 2 cut(s) 430, 460
TaiI ACGT 1 cut(s) 489
TaqI TCGA 4 cut(s) 114, 151, 451, 684
TasI AATT 1 cut(s) 447
TatI WGTACW 2 cut(s) 103, 717
TauI GCSGC 2 cut(s) 233, 276
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TscAI CASTG 3 cut(s) 396, 419, 480
TspDTI ATGAA 3 cut(s) 63, 723, 780
TspRI CASTG 3 cut(s) 396, 419, 480
Van91I CCANNNNNTGG 1 cut(s) 466
VpaK11BI GGWCC 1 cut(s) 523
XapI RAATTY 1 cut(s) 447
XceI RCATGY 1 cut(s) 783
XhoI CTCGAG 1 cut(s) 150
XmiI GTMKAC 1 cut(s) 436
ZrmI AGTACT 1 cut(s) 719
Zsp2I ATGCAT 2 cut(s) 123, 781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.