Rh5BG006700

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0002139
Physical Location & Seq
Reverse (-)
482891 .. 483917
1027 bp
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UTR
Exon/CDS
Intron
Rh5BG006700.1

Sequence Viewer

Length: 426 bp
ATGGGTGAAGAAAACAAACCAGAGGAGAAGAAGGAGGAAGAAAAGAAGGAGGAAGAGAAGAAGGTAGAAGAGCCACCGGAGATTGTGCTCAAGGTCGATATGCATTGTGAAGCTTGCGCCAGGAAAGTTGCTAGAGCGTTGAAGGGTTTTGAAGGAGTTGAGGATGTAACTACAGACAGCAAAGCGAGCAAGGTGGTGGTGAAAGGCAAAGCAGCAGACCCCATAAAGGTATGCGAGAGATTACAAAAGAAAAGTGGCAAAAAAGTGGAGCTGATTTCACCTTTGCCTAAACCAGTCGAGGAGGAGAAGAAAGAGGAGCCCAAAGAACCAGAGAACAAGGAGGAGAAAAAAGAGGAGGTAACTAATTGCTCCATCTCTTACTTTAACCAACTCCATCAATTCCTTCATTCTTTTTTACCTCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

141

Amino Acids

16.15

Weight (kDa)

5.74

Isoelectric Point (pI)

55.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 84 8.6e-14 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 2 cut(s) 142, 152
AjnI CCWGG 1 cut(s) 119
AluBI AGCT 2 cut(s) 113, 271
AluI AGCT 2 cut(s) 113, 271
Alw21I GWGCWC 1 cut(s) 90
ApeKI GCWGC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 17, 211, 270
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 380, 402
BciT130I CCWGG 1 cut(s) 121
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 171
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 1 cut(s) 121
BmiI GGNNCC 1 cut(s) 318
BmrFI CCNGG 1 cut(s) 121
BpuEI CTTGAG 1 cut(s) 74
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 121
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 226
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 6 cut(s) 38, 314, 317, 329, 356, 368
BseXI GCAGC 1 cut(s) 224
BsiHKAI GWGCWC 1 cut(s) 90
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 1 cut(s) 226
Bsp1286I GDGCHC 2 cut(s) 90, 321
BspLI GGNNCC 1 cut(s) 318
BspQI GCTCTTC 1 cut(s) 63
BsrI ACTGG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 121
Bst6I CTCTTC 2 cut(s) 48, 63
BstC8I GCNNGC 2 cut(s) 115, 187
BstF5I GGATG 1 cut(s) 169
BstHHI GCGC 1 cut(s) 119
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 1 cut(s) 121
BstSCI CCNGG 1 cut(s) 119
BstSFI CTRYAG 1 cut(s) 171
BstV1I GCAGC 1 cut(s) 224
BtsCI GGATG 1 cut(s) 169
Cac8I GCNNGC 2 cut(s) 115, 187
CfoI GCGC 1 cut(s) 119
CviJI RGCY 4 cut(s) 73, 113, 271, 319
CviKI_1 RGCY 4 cut(s) 73, 113, 271, 319
Eam1104I CTCTTC 2 cut(s) 48, 63
EarI CTCTTC 2 cut(s) 48, 63
Eco24I GRGCYC 1 cut(s) 321
EcoRII CCWGG 1 cut(s) 119
EcoT22I ATGCAT 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 321
FaiI YATR 3 cut(s) 101, 224, 232
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 118
GluI GCNGC 1 cut(s) 213
HapII CCGG 1 cut(s) 77
HhaI GCGC 1 cut(s) 119
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 1 cut(s) 111
HpaII CCGG 1 cut(s) 77
HphI GGTGA 3 cut(s) 17, 211, 270
HpyAV CCTTC 6 cut(s) 25, 40, 55, 136, 146, 413
HpyCH4V TGCA 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HspAI GCGC 1 cut(s) 117
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 3 cut(s) 268, 316, 374
LpnPI CCDG 6 cut(s) 33, 90, 106, 133, 306, 342
Lsp1109I GCAGC 1 cut(s) 224
MaeI CTAG 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 166, 358
MboII GAAGA 7 cut(s) 20, 40, 50, 65, 70, 80, 319
MhlI GDGCHC 2 cut(s) 90, 321
MluCI AATT 2 cut(s) 364, 398
Mph1103I ATGCAT 1 cut(s) 105
MseI TTAA 1 cut(s) 384
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 1 cut(s) 121
MvaI CCWGG 1 cut(s) 121
MwoI GCNNNNNNNGC 1 cut(s) 186
NlaIV GGNNCC 1 cut(s) 318
NsiI ATGCAT 1 cut(s) 105
PciSI GCTCTTC 1 cut(s) 63
PkrI GCNGC 1 cut(s) 214
Psp6I CCWGG 1 cut(s) 119
PspGI CCWGG 1 cut(s) 119
PspN4I GGNNCC 1 cut(s) 318
SapI GCTCTTC 1 cut(s) 63
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 1 cut(s) 213
ScrFI CCNGG 1 cut(s) 121
SduI GDGCHC 2 cut(s) 90, 321
SetI ASST 9 cut(s) 66, 96, 115, 195, 231, 273, 283, 360, 421
SfcI CTRYAG 1 cut(s) 171
SmlI CTYRAG 1 cut(s) 89
SmoI CTYRAG 1 cut(s) 89
Sse9I AATT 2 cut(s) 364, 398
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 119
TaqI TCGA 2 cut(s) 96, 297
TasI AATT 2 cut(s) 364, 398
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 395
XspI CTAG 1 cut(s) 132
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.