Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0002139

Physical Location & Seq

Genomic Coordinates Reverse (-)

44875788 .. 44880884

Gene Structure 5097 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1189000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 801 bp

ATGGGCGAAGAGAAGAAAGAGAAAGAGGAAGAGAAGAAGGAAGAAAGCAAAGATGAAAAGAAAGAAGAAGAGAAAAAGGAAGAAAAGAAAGAAGAGCCTCCGGAGATTGTACTCAAGGTCGATATGCATTGTGAAGCTTGCGCAAGGAAAGTTGCTCGAGCCTTGAAGGGTTTTGAAGGAGTGGAGGATGTAACGACAGACAGCAAGGCGAGTAAGGTGGTGGTGAAAGGGAAGGCGGCAGACCCCATAAAGGTCTGCGAAAGATTACAAAGGAAAAGCGGCAAAAAAGTAGAGCTCATTTCACCTTTGCCTAAACCTCCCGTGGAGAAGGAGGAAGAACAAGTTAAGGAAGCCGACAAGGAAGAGAAAAAAGAGGAGCCTCCTGCTGTTGTTTCAGTGGTATTGCAAGTTCGAATGCACTGCGATGCGTGCGCCCAAGTTCTACAGAAGCGAATTCGAAAGATCCAAGGTGTGGAGTCAGTGGAAACAGACGTAGGCAACAACAAGGTTGTAGTAAAAGGCGTGATGGACCCGGCGAAGCTTTCGGAGGATGTGTATAAAAAAACCAGGAAGCAGGTTTCTGTTGTGAAGGAGGATGAGAAGAAAGACGAAAAGAAAGAAGACGAGAACAAGAAGGAAGGAGAGAAGAAAGAAGGGGAGGAAGACAAGCAGGGAGAGGACAACAAGGTCGATATAAAGCGAAGCGATTATTGGCCAACAAAGTACTACTCGGAATATTCATCGTATCCCCACCCTCAAATTTTCAGCGATGAAAACCCTAATGCATGCTCTGTTATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

HMA

Protein Analysis

267

Amino Acids

30.45

Weight (kDa)

5.57

Isoelectric Point (pI)

55.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	38 - 92	2.9e-13	Heavy-metal-associated domain
HMA	PF00403	135 - 182	1.8e-10	Heavy-metal-associated domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0002139)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G50740 AT5G50740 AT5G50740 AT5G50740 AT5G50740
fragaria_vesca	FvH4_3g00420 FvH4_5g08650 FvH4_5g08650 FvH4_5g08650 FvH4_6g37481
malus_domestica	MD00G1189000.v1.1 MD05G1360600.v1.1 MD06G1138200.v1.1 MD14G1154200.v1.1
prunus_persica	Prupe.4G004800_v2.0.a1 Prupe.4G004800_v2.0.a1 Prupe.4G004800_v2.0.a1 Prupe.5G148500_v2.0.a1 Prupe.5G148500_v2.0.a1
pyrus_communis	pycom05g32740 pycom06g12950 pycom10g28780 pycom14g12820
rosa_chinensis	RchiOBHm_Chr5g0000611 RchiOBHm_Chr7g0189731
rosa_laevigata	RLG00000004594 RLG00000030881
rosa_multiflora	Rmu_co8223778.1_g000001 Rmu_co8488275.1_g000001 Rmu_sc0001924.1_g000028 Rmu_sc0040344.1_g000001
rosa_roxburghii	Rroxscaffold_1G00075600 Rroxscaffold_3G00265220
rosa_rugosa	Rorug04G0385400 Rorug06G0499400 Rorug06G0499400
rosa_samantha	Rh5AG005100 Rh5BG006700 Rh5CG005500 Rh5DG005400 Rh7AG104700 Rh7CG109200
rosa_wichuraiana	Rw5G000500 Rw7G009060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	686
Acc16I	TGCGCA	1 cut(s)	142
Acc36I	ACCTGC	1 cut(s)	565
AccB7I	CCANNNNNTGG	1 cut(s)	472
AccIII	TCCGGA	1 cut(s)	100
AciI	CCGC	2 cut(s)	236, 279
AclWI	GGATC	1 cut(s)	457
AcoI	YGGCCR	1 cut(s)	713
AcsI	RAATTY	2 cut(s)	453, 759
AfaI	GTAC	2 cut(s)	111, 725
AfiI	CCNNNNNNNGG	2 cut(s)	250, 472
AgsI	TTSAA	2 cut(s)	166, 176
AjnI	CCWGG	1 cut(s)	566
AjuI	GAANNNNNNNTTGG	2 cut(s)	694, 726
AluBI	AGCT	3 cut(s)	137, 295, 541
AluI	AGCT	3 cut(s)	137, 295, 541
Alw21I	GWGCWC	1 cut(s)	297
AlwI	GGATC	1 cut(s)	457
Ama87I	CYCGRG	1 cut(s)	156
Aor13HI	TCCGGA	1 cut(s)	100
AoxI	GGCC	1 cut(s)	713
ApoI	RAATTY	2 cut(s)	453, 759
AspLEI	GCGC	2 cut(s)	143, 434
AspS9I	GGNCC	1 cut(s)	529
AsuC2I	CCSGG	1 cut(s)	533
AsuHPI	GGTGA	2 cut(s)	235, 294
AsuII	TTCGAA	2 cut(s)	412, 457
AvaI	CYCGRG	1 cut(s)	156
AvaII	GGWCC	1 cut(s)	529
BalI	TGGCCA	1 cut(s)	715
BanII	GRGCYC	1 cut(s)	297
BbsI	GAAGAC	2 cut(s)	627, 669
Bbv12I	GWGCWC	1 cut(s)	297
BccI	CCATC	1 cut(s)	520
BcgI	CGANNNNNNTGC	2 cut(s)	402, 436
BciT130I	CCWGG	1 cut(s)	568
BciVI	GTATCC	1 cut(s)	756
BcnI	CCSGG	1 cut(s)	533
BfmI	CTRYAG	1 cut(s)	443
BfuAI	ACCTGC	1 cut(s)	565
BfuI	GTATCC	1 cut(s)	756
BisI	GCNGC	2 cut(s)	237, 280
BlsI	GCNGC	2 cut(s)	238, 281
BmcAI	AGTACT	1 cut(s)	725
Bme1390I	CCNGG	2 cut(s)	533, 568
Bme18I	GGWCC	1 cut(s)	529
BmeT110I	CYCGRG	1 cut(s)	156
BmgT120I	GGNCC	1 cut(s)	529
BmiI	GGNNCC	2 cut(s)	378, 531
BmrFI	CCNGG	2 cut(s)	533, 568
BmsI	GCATC	1 cut(s)	415
BpiI	GAAGAC	2 cut(s)	627, 669
Bpu14I	TTCGAA	2 cut(s)	412, 457
BpuEI	CTTGAG	1 cut(s)	98
BpuMI	CCSGG	1 cut(s)	533
BsaJI	CCNNGG	2 cut(s)	321, 466
BsaWI	WCCGGW	1 cut(s)	100
BsaXI	ACNNNNNCTCC	2 cut(s)	539, 569
Bsc4I	CCNNNNNNNGG	2 cut(s)	250, 472
BseAI	TCCGGA	1 cut(s)	100
BseBI	CCWGG	1 cut(s)	568
BseDI	CCNNGG	2 cut(s)	321, 466
BseGI	GGATG	3 cut(s)	193, 556, 601
BseLI	CCNNNNNNNGG	2 cut(s)	250, 472
BseRI	GAGGAG	1 cut(s)	389
BshFI	GGCC	1 cut(s)	715
BsiHKAI	GWGCWC	1 cut(s)	297
BsiHKCI	CYCGRG	1 cut(s)	156
BsiSI	CCGG	2 cut(s)	101, 533
BslI	CCNNNNNNNGG	2 cut(s)	250, 472
BsmI	GAATGC	1 cut(s)	420
BsnI	GGCC	1 cut(s)	715
BsoBI	CYCGRG	1 cut(s)	156
Bsp119I	TTCGAA	2 cut(s)	412, 457
Bsp1286I	GDGCHC	1 cut(s)	297
Bsp13I	TCCGGA	1 cut(s)	100
Bsp143I	GATC	1 cut(s)	462
BspACI	CCGC	2 cut(s)	236, 279
BspANI	GGCC	1 cut(s)	715
BspEI	TCCGGA	1 cut(s)	100
BspLI	GGNNCC	2 cut(s)	378, 531
BspMI	ACCTGC	1 cut(s)	565
BspPI	GGATC	1 cut(s)	457
BspQI	GCTCTTC	1 cut(s)	87
BspT104I	TTCGAA	2 cut(s)	412, 457
BssECI	CCNNGG	2 cut(s)	321, 466
BssMI	GATC	1 cut(s)	462
BssT1I	CCWWGG	1 cut(s)	466
Bst2UI	CCWGG	1 cut(s)	568
Bst6I	CTCTTC	5 cut(s)	3, 24, 63, 87, 357
BstBI	TTCGAA	2 cut(s)	412, 457
BstC8I	GCNNGC	3 cut(s)	139, 430, 787
BstDSI	CCRYGG	1 cut(s)	321
BstF5I	GGATG	3 cut(s)	193, 556, 601
BstHHI	GCGC	2 cut(s)	143, 434
BstKTI	GATC	1 cut(s)	465
BstMBI	GATC	1 cut(s)	462
BstMWI	GCNNNNNNNGC	1 cut(s)	429
BstNI	CCWGG	1 cut(s)	568
BstNSI	RCATGY	1 cut(s)	789
BstSCI	CCNGG	2 cut(s)	531, 566
BstSFI	CTRYAG	1 cut(s)	443
BstV2I	GAAGAC	2 cut(s)	627, 669
BstX2I	RGATCY	1 cut(s)	462
BstYI	RGATCY	1 cut(s)	462
BsuI	GTATCC	1 cut(s)	756
BsuRI	GGCC	1 cut(s)	715
BtgI	CCRYGG	1 cut(s)	321
BtgZI	GCGATG	2 cut(s)	438, 783
BtsCI	GGATG	3 cut(s)	193, 556, 601
BtsI	GCAGTG	1 cut(s)	418
BtsIMutI	CAGTG	3 cut(s)	402, 418, 486
BveI	ACCTGC	1 cut(s)	565
Cac8I	GCNNGC	3 cut(s)	139, 430, 787
CfoI	GCGC	2 cut(s)	143, 434
Cfr13I	GGNCC	1 cut(s)	529
Csp6I	GTAC	2 cut(s)	110, 724
CviAII	CATG	1 cut(s)	786
CviJI	RGCY	8 cut(s)	97, 137, 161, 295, 353, 379, 541, 715
CviKI_1	RGCY	8 cut(s)	97, 137, 161, 295, 353, 379, 541, 715
CviQI	GTAC	2 cut(s)	110, 724
DpnI	GATC	1 cut(s)	464
DpnII	GATC	1 cut(s)	462
DrdI	GACNNNNNNGTC	1 cut(s)	686
DseDI	GACNNNNNNGTC	1 cut(s)	686
EaeI	YGGCCR	1 cut(s)	713
Eam1104I	CTCTTC	5 cut(s)	3, 24, 63, 87, 357
EarI	CTCTTC	5 cut(s)	3, 24, 63, 87, 357
Ecl136II	GAGCTC	1 cut(s)	295
Eco130I	CCWWGG	1 cut(s)	466
Eco24I	GRGCYC	1 cut(s)	297
Eco47I	GGWCC	1 cut(s)	529
Eco53kI	GAGCTC	1 cut(s)	295
Eco88I	CYCGRG	1 cut(s)	156
EcoICRI	GAGCTC	1 cut(s)	295
EcoRI	GAATTC	1 cut(s)	453
EcoRII	CCWGG	1 cut(s)	566
EcoT14I	CCWWGG	1 cut(s)	466
EcoT22I	ATGCAT	2 cut(s)	129, 787
EcoT38I	GRGCYC	1 cut(s)	297
ErhI	CCWWGG	1 cut(s)	466
FaeI	CATG	1 cut(s)	789
FaiI	YATR	6 cut(s)	125, 248, 558, 695, 787, 797
FatI	CATG	1 cut(s)	785
Fnu4HI	GCNGC	2 cut(s)	237, 280
FokI	GGATG	3 cut(s)	200, 563, 608
FriOI	GRGCYC	1 cut(s)	297
Fsp4HI	GCNGC	2 cut(s)	237, 280
FspI	TGCGCA	1 cut(s)	142
GlaI	GCGC	2 cut(s)	142, 433
GluI	GCNGC	2 cut(s)	237, 280
HaeIII	GGCC	1 cut(s)	715
HapII	CCGG	2 cut(s)	101, 533
HhaI	GCGC	2 cut(s)	143, 434
Hin1II	CATG	1 cut(s)	789
Hin6I	GCGC	2 cut(s)	141, 432
HinP1I	GCGC	2 cut(s)	141, 432
HindIII	AAGCTT	2 cut(s)	135, 539
HinfI	GANTC	1 cut(s)	476
HpaII	CCGG	2 cut(s)	101, 533
HphI	GGTGA	2 cut(s)	235, 294
Hpy188I	TCNGA	2 cut(s)	547, 733
Hpy188III	TCNNGA	1 cut(s)	101
HpyAV	CCTTC	9 cut(s)	31, 160, 170, 226, 322, 583, 628, 632, 647
HpyCH4IV	ACGT	1 cut(s)	492
HpyCH4V	TGCA	4 cut(s)	127, 406, 418, 785
HpyF10VI	GCNNNNNNNGC	1 cut(s)	429
HpySE526I	ACGT	1 cut(s)	492
Hsp92II	CATG	1 cut(s)	789
HspAI	GCGC	2 cut(s)	141, 432
Kpn2I	TCCGGA	1 cut(s)	100
Kzo9I	GATC	1 cut(s)	462
LguI	GCTCTTC	1 cut(s)	87
LmnI	GCTCC	1 cut(s)	376
LpnPI	CCDG	7 cut(s)	114, 396, 546, 553, 560, 580, 656
LweI	GCATC	1 cut(s)	415
MaeII	ACGT	1 cut(s)	492
MaeIII	GTNAC	1 cut(s)	190
MalI	GATC	1 cut(s)	464
MboI	GATC	1 cut(s)	462
MflI	RGATCY	1 cut(s)	462
MhlI	GDGCHC	1 cut(s)	297
MlsI	TGGCCA	1 cut(s)	715
MluCI	AATT	2 cut(s)	453, 759
MluNI	TGGCCA	1 cut(s)	715
MlyI	GAGTC	1 cut(s)	485
Mox20I	TGGCCA	1 cut(s)	715
Mph1103I	ATGCAT	2 cut(s)	129, 787
MroI	TCCGGA	1 cut(s)	100
MscI	TGGCCA	1 cut(s)	715
MseI	TTAA	1 cut(s)	345
MslI	CAYNNNNRTG	1 cut(s)	423
Msp20I	TGGCCA	1 cut(s)	715
MspI	CCGG	2 cut(s)	101, 533
MspR9I	CCNGG	2 cut(s)	533, 568
Mva1269I	GAATGC	1 cut(s)	420
MvaI	CCWGG	1 cut(s)	568
MwoI	GCNNNNNNNGC	1 cut(s)	429
NciI	CCSGG	1 cut(s)	533
NdeII	GATC	1 cut(s)	462
NlaIII	CATG	1 cut(s)	789
NlaIV	GGNNCC	2 cut(s)	378, 531
NsbI	TGCGCA	1 cut(s)	142
NsiI	ATGCAT	2 cut(s)	129, 787
NspI	RCATGY	1 cut(s)	789
NspV	TTCGAA	2 cut(s)	412, 457
PaeI	GCATGC	1 cut(s)	789
PaeR7I	CTCGAG	1 cut(s)	156
PciSI	GCTCTTC	1 cut(s)	87
PctI	GAATGC	1 cut(s)	420
PflMI	CCANNNNNTGG	1 cut(s)	472
PkrI	GCNGC	2 cut(s)	238, 281
PleI	GAGTC	1 cut(s)	484
PpsI	GAGTC	1 cut(s)	484
Psp124BI	GAGCTC	1 cut(s)	297
Psp6I	CCWGG	1 cut(s)	566
PspGI	CCWGG	1 cut(s)	566
PspN4I	GGNNCC	2 cut(s)	378, 531
PspPI	GGNCC	1 cut(s)	529
PspXI	VCTCGAGB	1 cut(s)	156
PsrI	GAACNNNNNNTAC	2 cut(s)	393, 425
PsuI	RGATCY	1 cut(s)	462
RsaI	GTAC	2 cut(s)	111, 725
RsaNI	GTAC	2 cut(s)	110, 724
RseI	CAYNNNNRTG	1 cut(s)	423
SacI	GAGCTC	1 cut(s)	297
SapI	GCTCTTC	1 cut(s)	87
SaqAI	TTAA	1 cut(s)	345
SatI	GCNGC	2 cut(s)	237, 280
Sau3AI	GATC	1 cut(s)	462
Sau96I	GGNCC	1 cut(s)	529
ScaI	AGTACT	1 cut(s)	725
SchI	GAGTC	1 cut(s)	485
ScrFI	CCNGG	2 cut(s)	533, 568
SduI	GDGCHC	1 cut(s)	297
SfaNI	GCATC	1 cut(s)	415
SfcI	CTRYAG	1 cut(s)	443
Sfr274I	CTCGAG	1 cut(s)	156
SfuI	TTCGAA	2 cut(s)	412, 457
SinI	GGWCC	1 cut(s)	529
SlaI	CTCGAG	1 cut(s)	156
SmiMI	CAYNNNNRTG	1 cut(s)	423
SmlI	CTYRAG	2 cut(s)	113, 156
SmoI	CTYRAG	2 cut(s)	113, 156
SphI	GCATGC	1 cut(s)	789
Sse9I	AATT	2 cut(s)	453, 759
SsiI	CCGC	2 cut(s)	236, 279
SspI	AATATT	1 cut(s)	737
SstI	GAGCTC	1 cut(s)	297
StyD4I	CCNGG	2 cut(s)	531, 566
StyI	CCWWGG	1 cut(s)	466
TaiI	ACGT	1 cut(s)	495
TaqI	TCGA	5 cut(s)	120, 157, 412, 457, 690
TasI	AATT	2 cut(s)	453, 759
TatI	WGTACW	2 cut(s)	109, 723
TauI	GCSGC	2 cut(s)	239, 282
Tru1I	TTAA	1 cut(s)	345
Tru9I	TTAA	1 cut(s)	345
TscAI	CASTG	3 cut(s)	402, 425, 486
TspDTI	ATGAA	3 cut(s)	69, 729, 786
TspRI	CASTG	3 cut(s)	402, 425, 486
Van91I	CCANNNNNTGG	1 cut(s)	472
VpaK11BI	GGWCC	1 cut(s)	529
XapI	RAATTY	2 cut(s)	453, 759
XceI	RCATGY	1 cut(s)	789
XhoI	CTCGAG	1 cut(s)	156
ZrmI	AGTACT	1 cut(s)	725
Zsp2I	ATGCAT	2 cut(s)	129, 787

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.