Rroxscaffold_1G00075600

Heavy-metal-associated domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0002139
Physical Location & Seq
Forward (+)
96285631 .. 96288951
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075600.1

Sequence Viewer

Length: 804 bp
ATGGGTGAAGAAAACAAACCAGAGGAGAAGAAGGAGGAAGAAAAGAAGGAGGAAGAGAAGAAGGTAGAAGAGCCACCGGAGATTGTGCTCAAGGTCGATATGCATTGTGAAGCTTGCGCCAGGAAAGTTGCTAGAGCCTTGAAGGGTTTTGAAGGAGTTGAGGATGTAACTACAGACAGCAAAGCGAGCAAGGTGGTGGTGAAAGGCAAAGCAGCAGACCCCATAAAGGTATGCGAGAGATTACAAAAGAAAAGTGGCAAAAAAGTGGAGTTGATTTCACCTTTGCCTAAACCAGTCGAGGAGAAGAAGAAAGAGGAGCCCAAAGAACCAGAGAACAAGGAGGAGAAAAAAGAGGAGCCTCCCGCTGTTGTTACAGTGATTTTGAAAGTTCGAATGCACTGCGAAGCATGTGCTCAAGTTTTACAGAAGCGAATCCGAAAGATCCAAGGTGTGGAATCCGTAGTAACAGACGTTGGTAACGACCAGGTTGTAGTAACAGGTGTTGTGGATCCAGCCAAGCTTGCAGATGATGTCTACAAAAAAACCAGAAAGCACGTTTCCATTGTGCCCAAGGAGGAAGAAAAGAAGGAAGAAGAAAAGAAAGAAGAGGGCAAGAAAGAAGAGACCAAAGATGGAGGAGGGGAGAAGAAAGAAGGGGAAACAGAAGAAGGCAAGGGATCCGATGATAACAAGAGTTTTGATATCAAGCGGAGTGAATATTGGCCATCAAAGTACTACTCGGATTATGCAGATACCCCTCAGATTTTCAGTGATGAGAACCCTAATGCCTGCTCTGTCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HMA

Protein Analysis

267

Amino Acids

30.13

Weight (kDa)

5.47

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HMA PF00403 30 - 84 3.2e-13 Heavy-metal-associated domain
HMA PF00403 129 - 175 7.1e-11 Heavy-metal-associated domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 451
AccI GTMKAC 1 cut(s) 534
AciI CCGC 2 cut(s) 363, 709
AclWI GGATC 5 cut(s) 436, 503, 516, 672, 685
AcoI YGGCCR 1 cut(s) 722
AfaI GTAC 1 cut(s) 734
AfiI CCNNNNNNNGG 2 cut(s) 226, 451
AgsI TTSAA 3 cut(s) 142, 152, 385
AjnI CCWGG 2 cut(s) 119, 483
AluBI AGCT 2 cut(s) 113, 520
AluI AGCT 2 cut(s) 113, 520
Alw21I GWGCWC 2 cut(s) 90, 415
Alw26I GTCTC 1 cut(s) 617
AlwI GGATC 5 cut(s) 436, 503, 516, 672, 685
AoxI GGCC 1 cut(s) 722
ApeKI GCWGC 1 cut(s) 212
AspLEI GCGC 1 cut(s) 119
AsuHPI GGTGA 3 cut(s) 17, 211, 270
AsuII TTCGAA 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 570
BalI TGGCCA 1 cut(s) 724
BamHI GGATCC 2 cut(s) 508, 677
BanII GRGCYC 1 cut(s) 321
Bbv12I GWGCWC 2 cut(s) 90, 415
BbvI GCAGC 1 cut(s) 224
BccI CCATC 2 cut(s) 626, 733
BcgI CGANNNNNNTGC 4 cut(s) 381, 392, 415, 426
BciT130I CCWGG 2 cut(s) 121, 485
BcoDI GTCTC 1 cut(s) 617
BfaI CTAG 1 cut(s) 132
BfmI CTRYAG 1 cut(s) 171
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
BmcAI AGTACT 1 cut(s) 734
Bme1390I CCNGG 2 cut(s) 121, 485
BmiI GGNNCC 4 cut(s) 318, 357, 510, 679
BmrFI CCNGG 2 cut(s) 121, 485
Bpu14I TTCGAA 1 cut(s) 391
BpuEI CTTGAG 2 cut(s) 74, 399
BsaI GGTCTC 1 cut(s) 617
BsaJI CCNNGG 2 cut(s) 445, 570
BsaWI WCCGGW 1 cut(s) 76
Bsc4I CCNNNNNNNGG 2 cut(s) 226, 451
Bse1I ACTGG 1 cut(s) 293
BseBI CCWGG 2 cut(s) 121, 485
BseDI CCNNGG 2 cut(s) 445, 570
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 2 cut(s) 226, 451
BseMII CTCAG 1 cut(s) 773
BseNI ACTGG 1 cut(s) 293
BseRI GAGGAG 6 cut(s) 38, 314, 329, 356, 368, 651
BseSI GKGCMC 1 cut(s) 570
BseXI GCAGC 1 cut(s) 224
BshFI GGCC 1 cut(s) 724
BsiHKAI GWGCWC 2 cut(s) 90, 415
BsiSI CCGG 1 cut(s) 77
BslI CCNNNNNNNGG 2 cut(s) 226, 451
BsmAI GTCTC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 399
BsnI GGCC 1 cut(s) 724
Bso31I GGTCTC 1 cut(s) 617
Bsp119I TTCGAA 1 cut(s) 391
Bsp1286I GDGCHC 4 cut(s) 90, 321, 415, 570
Bsp143I GATC 3 cut(s) 441, 508, 677
BspACI CCGC 2 cut(s) 363, 709
BspANI GGCC 1 cut(s) 724
BspCNI CTCAG 1 cut(s) 772
BspLI GGNNCC 4 cut(s) 318, 357, 510, 679
BspPI GGATC 5 cut(s) 436, 503, 516, 672, 685
BspQI GCTCTTC 1 cut(s) 63
BspT104I TTCGAA 1 cut(s) 391
BspTNI GGTCTC 1 cut(s) 617
BsrI ACTGG 1 cut(s) 293
BssECI CCNNGG 2 cut(s) 445, 570
BssMI GATC 3 cut(s) 441, 508, 677
BssT1I CCWWGG 2 cut(s) 445, 570
Bst2UI CCWGG 2 cut(s) 121, 485
Bst4CI ACNGT 1 cut(s) 376
Bst6I CTCTTC 4 cut(s) 48, 63, 600, 615
BstBI TTCGAA 1 cut(s) 391
BstC8I GCNNGC 4 cut(s) 115, 187, 522, 790
BstDEI CTNAG 1 cut(s) 759
BstF5I GGATG 1 cut(s) 169
BstHHI GCGC 1 cut(s) 119
BstKTI GATC 3 cut(s) 444, 511, 680
BstMAI GTCTC 1 cut(s) 617
BstMBI GATC 3 cut(s) 441, 508, 677
BstMWI GCNNNNNNNGC 2 cut(s) 186, 521
BstNI CCWGG 2 cut(s) 121, 485
BstNSI RCATGY 1 cut(s) 411
BstSCI CCNGG 2 cut(s) 119, 483
BstSFI CTRYAG 1 cut(s) 171
BstSLI GKGCMC 1 cut(s) 570
BstV1I GCAGC 1 cut(s) 224
BstX2I RGATCY 3 cut(s) 441, 508, 677
BstYI RGATCY 3 cut(s) 441, 508, 677
BsuRI GGCC 1 cut(s) 724
BtsCI GGATG 1 cut(s) 169
BtsI GCAGTG 1 cut(s) 397
BtsIMutI CAGTG 3 cut(s) 381, 397, 775
Cac8I GCNNGC 4 cut(s) 115, 187, 522, 790
CfoI GCGC 1 cut(s) 119
CsiI ACCWGGT 1 cut(s) 483
Csp6I GTAC 1 cut(s) 733
CviAII CATG 2 cut(s) 408, 799
CviJI RGCY 8 cut(s) 73, 113, 137, 319, 358, 515, 520, 724
CviKI_1 RGCY 8 cut(s) 73, 113, 137, 319, 358, 515, 520, 724
CviQI GTAC 1 cut(s) 733
DdeI CTNAG 1 cut(s) 759
DpnI GATC 3 cut(s) 443, 510, 679
DpnII GATC 3 cut(s) 441, 508, 677
EaeI YGGCCR 1 cut(s) 722
Eam1104I CTCTTC 4 cut(s) 48, 63, 600, 615
EarI CTCTTC 4 cut(s) 48, 63, 600, 615
Eco130I CCWWGG 2 cut(s) 445, 570
Eco24I GRGCYC 1 cut(s) 321
Eco31I GGTCTC 1 cut(s) 617
Eco32I GATATC 1 cut(s) 703
EcoRII CCWGG 2 cut(s) 119, 483
EcoRV GATATC 1 cut(s) 703
EcoT14I CCWWGG 2 cut(s) 445, 570
EcoT22I ATGCAT 1 cut(s) 105
EcoT38I GRGCYC 1 cut(s) 321
ErhI CCWWGG 2 cut(s) 445, 570
FaeI CATG 2 cut(s) 411, 802
FaiI YATR 6 cut(s) 101, 224, 232, 409, 747, 800
FatI CATG 2 cut(s) 407, 798
FauI CCCGC 1 cut(s) 370
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 176
FriOI GRGCYC 1 cut(s) 321
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 132
GlaI GCGC 1 cut(s) 118
GluI GCNGC 1 cut(s) 213
HaeIII GGCC 1 cut(s) 724
HapII CCGG 1 cut(s) 77
HhaI GCGC 1 cut(s) 119
Hin1II CATG 2 cut(s) 411, 802
Hin6I GCGC 1 cut(s) 117
HinP1I GCGC 1 cut(s) 117
HindIII AAGCTT 2 cut(s) 111, 518
HinfI GANTC 2 cut(s) 432, 455
HpaII CCGG 1 cut(s) 77
HphI GGTGA 3 cut(s) 17, 211, 270
Hpy166II GTNNAC 1 cut(s) 535
Hpy188I TCNGA 4 cut(s) 437, 682, 742, 762
Hpy8I GTNNAC 1 cut(s) 535
HpyAV CCTTC 8 cut(s) 25, 40, 55, 136, 146, 580, 647, 662
HpyCH4III ACNGT 1 cut(s) 376
HpyCH4IV ACGT 2 cut(s) 471, 555
HpyCH4V TGCA 4 cut(s) 103, 397, 524, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 186, 521
HpyF3I CTNAG 1 cut(s) 759
HpySE526I ACGT 2 cut(s) 471, 555
Hsp92II CATG 2 cut(s) 411, 802
HspAI GCGC 1 cut(s) 117
Kzo9I GATC 3 cut(s) 441, 508, 677
LguI GCTCTTC 1 cut(s) 63
LmnI GCTCC 2 cut(s) 316, 355
Lsp1109I GCAGC 1 cut(s) 224
MabI ACCWGGT 1 cut(s) 483
MaeI CTAG 1 cut(s) 132
MaeII ACGT 2 cut(s) 471, 555
MaeIII GTNAC 5 cut(s) 166, 370, 463, 476, 493
MalI GATC 3 cut(s) 443, 510, 679
MboI GATC 3 cut(s) 441, 508, 677
MflI RGATCY 3 cut(s) 441, 508, 677
MhlI GDGCHC 4 cut(s) 90, 321, 415, 570
MlsI TGGCCA 1 cut(s) 724
MluNI TGGCCA 1 cut(s) 724
Mox20I TGGCCA 1 cut(s) 724
Mph1103I ATGCAT 1 cut(s) 105
MscI TGGCCA 1 cut(s) 724
Msp20I TGGCCA 1 cut(s) 724
MspA1I CMGCKG 1 cut(s) 365
MspI CCGG 1 cut(s) 77
MspR9I CCNGG 2 cut(s) 121, 485
Mva1269I GAATGC 1 cut(s) 399
MvaI CCWGG 2 cut(s) 121, 485
MwoI GCNNNNNNNGC 2 cut(s) 186, 521
NdeII GATC 3 cut(s) 441, 508, 677
NlaIII CATG 2 cut(s) 411, 802
NlaIV GGNNCC 4 cut(s) 318, 357, 510, 679
NsiI ATGCAT 1 cut(s) 105
NspI RCATGY 1 cut(s) 411
NspV TTCGAA 1 cut(s) 391
PciSI GCTCTTC 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 477
PctI GAATGC 1 cut(s) 399
PfeI GAWTC 2 cut(s) 432, 455
PflMI CCANNNNNTGG 1 cut(s) 451
PkrI GCNGC 1 cut(s) 214
Psp6I CCWGG 2 cut(s) 119, 483
PspGI CCWGG 2 cut(s) 119, 483
PspN4I GGNNCC 4 cut(s) 318, 357, 510, 679
PsuI RGATCY 3 cut(s) 441, 508, 677
RsaI GTAC 1 cut(s) 734
RsaNI GTAC 1 cut(s) 733
SapI GCTCTTC 1 cut(s) 63
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 3 cut(s) 441, 508, 677
ScaI AGTACT 1 cut(s) 734
ScrFI CCNGG 2 cut(s) 121, 485
SduI GDGCHC 4 cut(s) 90, 321, 415, 570
SexAI ACCWGGT 1 cut(s) 483
SfcI CTRYAG 1 cut(s) 171
SfuI TTCGAA 1 cut(s) 391
SmlI CTYRAG 2 cut(s) 89, 414
SmoI CTYRAG 2 cut(s) 89, 414
SsiI CCGC 2 cut(s) 363, 709
SspI AATATT 1 cut(s) 719
SspMI CTAG 1 cut(s) 132
StyD4I CCNGG 2 cut(s) 119, 483
StyI CCWWGG 2 cut(s) 445, 570
TaaI ACNGT 1 cut(s) 376
TaiI ACGT 2 cut(s) 474, 558
TaqI TCGA 3 cut(s) 96, 297, 391
TatI WGTACW 1 cut(s) 732
TfiI GAWTC 2 cut(s) 432, 455
TscAI CASTG 3 cut(s) 381, 404, 775
TseI GCWGC 1 cut(s) 212
TspGWI ACGGA 1 cut(s) 448
TspRI CASTG 3 cut(s) 381, 404, 775
Van91I CCANNNNNTGG 1 cut(s) 451
XceI RCATGY 1 cut(s) 411
XmiI GTMKAC 1 cut(s) 534
XspI CTAG 1 cut(s) 132
ZrmI AGTACT 1 cut(s) 734
Zsp2I ATGCAT 1 cut(s) 105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.