AT5G54110

MSP (Major sperm protein) domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0003521
Physical Location & Seq
Forward (+)
21958059 .. 21960634
2576 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54110.1

Sequence Viewer

Length: 801 bp
ATGCCAATAGGTGACCGTCAGAATCCGAGTGTGGAGAAGAAGAAGAACCTCTTCAGGCTATGTCCGTTTTGGCAGAGGAGAAGCACCACTTCTTCATCTTCAACACAGAATCCAAACCAAAACTACCGTAGCCGCCATGGAAATCGGAATACAGATATTTCCGCCGTCTCTAAGCCTCCGTTGACTATGTCTTCCGTCGCCAGATCACTTCTTCCAGCTCGCCGTCGTCTCCGACTAGATCCTTCTAGTTATCTGTATTTCCCTTATGAACCTGGAAAACAAGTTCGCAGCGCAATAAAGCTTAAGAACACTAGCAAATCTCATACTGCATTTAAGTTCCAAACAACGGCACCTAAGAGTTGTTATATGCGTCCTCCCGGAGGTGTTTTGGCTCCAGGAGAGAGTGTTTTTGCTACTGTGTTCAAGTTTGTGGAACATCCAGAGAACAATGAAAAGCAGCCGTTGAATCAGAAGAGCAAGGTTAAGTTTAAGATTATGAGCCTGAAAGTTAAACCAGGAGTAGAGTATGTGCCTGAGTTGTTTGATGAGCAAAAGGATCAAGTAGCAGTGGAGCAGGTTCTTCGGGTCATTTTTATTGATGCGGACCGCCCAAGTGCTGCATTGGAGAAACTGAAACGTCAATTGGATGAAGCTGAAGCTGCAGTTGAAGCAAGAAAGAAGCCTCCCCCAGAGACAGGGCCTCGAGTTGTCGGGGAGGGTCTTGTCATAGACGAATGGAAGGAGAGAAGAGAGAAGTACCTTGCTCGGCAACAAGTCGAATCCGTAGATTCTTTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

266

Amino Acids

30.25

Weight (kDa)

9.89

Isoelectric Point (pI)

51.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 77 - 188 4.5e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 349
AciI CCGC 4 cut(s) 133, 162, 602, 607
AclWI GGATC 2 cut(s) 233, 564
AcuI CTGAAG 2 cut(s) 37, 675
AfaI GTAC 1 cut(s) 758
AfiI CCNNNNNNNGG 3 cut(s) 346, 380, 695
AflII CTTAAG 1 cut(s) 302
AgsI TTSAA 4 cut(s) 102, 424, 466, 668
AjnI CCWGG 3 cut(s) 271, 394, 514
AjuI GAANNNNNNNTTGG 2 cut(s) 626, 658
AloI GAACNNNNNNTCC 2 cut(s) 267, 299
AluBI AGCT 4 cut(s) 218, 301, 653, 659
AluI AGCT 4 cut(s) 218, 301, 653, 659
Alw26I GTCTC 3 cut(s) 172, 233, 686
AlwI GGATC 2 cut(s) 233, 564
Ama87I CYCGRG 1 cut(s) 702
AoxI GGCC 1 cut(s) 698
ApeKI GCWGC 4 cut(s) 288, 457, 617, 659
Asp700I GAANNNNTTC 1 cut(s) 50
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 2 cut(s) 604, 698
AsuC2I CCSGG 1 cut(s) 378
AsuHPI GGTGA 1 cut(s) 23
AvaI CYCGRG 1 cut(s) 702
AvaII GGWCC 1 cut(s) 604
BanI GGYRCC 1 cut(s) 349
BbsI GAAGAC 1 cut(s) 183
BbvI GCAGC 4 cut(s) 300, 469, 604, 646
BceAI ACGGC 4 cut(s) 149, 207, 363, 445
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 3 cut(s) 273, 396, 516
BcnI CCSGG 1 cut(s) 378
BcoDI GTCTC 3 cut(s) 172, 233, 686
BfaI CTAG 3 cut(s) 236, 246, 312
BfmI CTRYAG 1 cut(s) 660
BfrI CTTAAG 1 cut(s) 302
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 5 cut(s) 133, 289, 458, 618, 660
BlsI GCNGC 5 cut(s) 134, 290, 459, 619, 661
Bme1390I CCNGG 4 cut(s) 273, 378, 396, 516
Bme18I GGWCC 1 cut(s) 604
BmeT110I CYCGRG 1 cut(s) 702
BmgT120I GGNCC 2 cut(s) 604, 698
BmiI GGNNCC 2 cut(s) 351, 393
BmrFI CCNGG 4 cut(s) 273, 378, 396, 516
BmsI GCATC 1 cut(s) 589
BpiI GAAGAC 1 cut(s) 183
BpmI CTGGAG 1 cut(s) 378
BpuMI CCSGG 1 cut(s) 378
BsaJI CCNNGG 1 cut(s) 136
BsaXI ACNNNNNCTCC 2 cut(s) 390, 420
Bsc4I CCNNNNNNNGG 3 cut(s) 346, 380, 695
BseBI CCWGG 3 cut(s) 273, 396, 516
BseDI CCNNGG 1 cut(s) 136
BseGI GGATG 2 cut(s) 436, 652
BseLI CCNNNNNNNGG 3 cut(s) 346, 380, 695
BseMII CTCAG 1 cut(s) 525
BseRI GAGGAG 1 cut(s) 91
BseXI GCAGC 4 cut(s) 300, 469, 604, 646
BshFI GGCC 1 cut(s) 700
BshNI GGYRCC 1 cut(s) 349
BsiHKCI CYCGRG 1 cut(s) 702
BsiSI CCGG 1 cut(s) 378
BslI CCNNNNNNNGG 3 cut(s) 346, 380, 695
BsmAI GTCTC 3 cut(s) 172, 233, 686
BsmBI CGTCTC 2 cut(s) 172, 233
BsnI GGCC 1 cut(s) 700
BsoBI CYCGRG 1 cut(s) 702
Bsp143I GATC 3 cut(s) 203, 238, 556
Bsp19I CCATGG 1 cut(s) 136
BspACI CCGC 4 cut(s) 133, 162, 602, 607
BspANI GGCC 1 cut(s) 700
BspCNI CTCAG 1 cut(s) 526
BspLI GGNNCC 2 cut(s) 351, 393
BspMAI CTGCAG 1 cut(s) 664
BspMI ACCTGC 1 cut(s) 565
BspPI GGATC 2 cut(s) 233, 564
BspQI GCTCTTC 1 cut(s) 467
BspT107I GGYRCC 1 cut(s) 349
BspTI CTTAAG 1 cut(s) 302
BssECI CCNNGG 1 cut(s) 136
BssMI GATC 3 cut(s) 203, 238, 556
BssT1I CCWWGG 1 cut(s) 136
Bst2UI CCWGG 3 cut(s) 273, 396, 516
Bst4CI ACNGT 3 cut(s) 17, 128, 418
Bst6I CTCTTC 3 cut(s) 56, 467, 742
BstAFI CTTAAG 1 cut(s) 302
BstC8I GCNNGC 1 cut(s) 220
BstDEI CTNAG 3 cut(s) 171, 354, 534
BstDSI CCRYGG 1 cut(s) 136
BstEII GGTNACC 1 cut(s) 11
BstENI CCTNNNNNAGG 1 cut(s) 378
BstF5I GGATG 2 cut(s) 436, 652
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 3 cut(s) 206, 241, 559
BstMAI GTCTC 3 cut(s) 172, 233, 686
BstMBI GATC 3 cut(s) 203, 238, 556
BstMWI GCNNNNNNNGC 2 cut(s) 659, 668
BstNI CCWGG 3 cut(s) 273, 396, 516
BstPI GGTNACC 1 cut(s) 11
BstSCI CCNGG 4 cut(s) 271, 376, 394, 514
BstSFI CTRYAG 1 cut(s) 660
BstV1I GCAGC 4 cut(s) 300, 469, 604, 646
BstV2I GAAGAC 1 cut(s) 183
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BsuRI GGCC 1 cut(s) 700
BtgI CCRYGG 1 cut(s) 136
BtsCI GGATG 2 cut(s) 436, 652
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 1 cut(s) 573
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 1 cut(s) 220
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 2 cut(s) 604, 698
CpoI CGGWCCG 1 cut(s) 604
CseI GACGC 1 cut(s) 359
Csp6I GTAC 1 cut(s) 757
CspI CGGWCCG 1 cut(s) 604
CviAII CATG 1 cut(s) 137
CviQI GTAC 1 cut(s) 757
DdeI CTNAG 3 cut(s) 171, 354, 534
DpnI GATC 3 cut(s) 205, 240, 558
DpnII GATC 3 cut(s) 203, 238, 556
Eam1104I CTCTTC 3 cut(s) 56, 467, 742
EarI CTCTTC 3 cut(s) 56, 467, 742
EciI GGCGGA 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 604
Eco57I CTGAAG 2 cut(s) 37, 675
Eco88I CYCGRG 1 cut(s) 702
Eco91I GGTNACC 1 cut(s) 11
EcoNI CCTNNNNNAGG 1 cut(s) 378
EcoO109I RGGNCCY 1 cut(s) 698
EcoO65I GGTNACC 1 cut(s) 11
EcoRII CCWGG 3 cut(s) 271, 394, 514
EcoT14I CCWWGG 1 cut(s) 136
ErhI CCWWGG 1 cut(s) 136
Esp3I CGTCTC 2 cut(s) 172, 233
FaeI CATG 1 cut(s) 140
FalI AAGNNNNNCTT 4 cut(s) 35, 67, 73, 105
FatI CATG 1 cut(s) 136
Fnu4HI GCNGC 5 cut(s) 133, 289, 458, 618, 660
FokI GGATG 2 cut(s) 423, 659
Fsp4HI GCNGC 5 cut(s) 133, 289, 458, 618, 660
FspBI CTAG 3 cut(s) 236, 246, 312
GlaI GCGC 1 cut(s) 292
GluI GCNGC 5 cut(s) 133, 289, 458, 618, 660
GsuI CTGGAG 1 cut(s) 378
HaeIII GGCC 1 cut(s) 700
HapII CCGG 1 cut(s) 378
HgaI GACGC 1 cut(s) 359
HhaI GCGC 1 cut(s) 293
Hin1II CATG 1 cut(s) 140
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HincII GTYRAC 1 cut(s) 183
HindII GTYRAC 1 cut(s) 183
HindIII AAGCTT 1 cut(s) 299
HinfI GANTC 5 cut(s) 22, 109, 466, 779, 788
HpaII CCGG 1 cut(s) 378
HphI GGTGA 1 cut(s) 23
Hpy166II GTNNAC 1 cut(s) 183
Hpy188I TCNGA 5 cut(s) 21, 27, 147, 233, 471
Hpy188III TCNNGA 1 cut(s) 440
Hpy8I GTNNAC 1 cut(s) 183
Hpy99I CGWCG 2 cut(s) 200, 228
HpyAV CCTTC 2 cut(s) 252, 733
HpyCH4III ACNGT 3 cut(s) 17, 128, 418
HpyCH4IV ACGT 1 cut(s) 637
HpyCH4V TGCA 3 cut(s) 329, 620, 662
HpyF10VI GCNNNNNNNGC 2 cut(s) 659, 668
HpyF3I CTNAG 3 cut(s) 171, 354, 534
HpySE526I ACGT 1 cut(s) 637
Hsp92II CATG 1 cut(s) 140
HspAI GCGC 1 cut(s) 291
Kzo9I GATC 3 cut(s) 203, 238, 556
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 2 cut(s) 397, 571
Lsp1109I GCAGC 4 cut(s) 300, 469, 604, 646
LweI GCATC 1 cut(s) 589
MaeI CTAG 3 cut(s) 236, 246, 312
MaeII ACGT 1 cut(s) 637
MaeIII GTNAC 1 cut(s) 11
MalI GATC 3 cut(s) 205, 240, 558
MboI GATC 3 cut(s) 203, 238, 556
MfeI CAATTG 1 cut(s) 641
MflI RGATCY 1 cut(s) 238
MluCI AATT 1 cut(s) 641
MmeI TCCRAC 1 cut(s) 256
MnlI CCTC 8 cut(s) 59, 69, 186, 374, 384, 693, 709, 711
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 5 cut(s) 303, 333, 483, 489, 510
MspCI CTTAAG 1 cut(s) 302
MspI CCGG 1 cut(s) 378
MspR9I CCNGG 4 cut(s) 273, 378, 396, 516
MunI CAATTG 1 cut(s) 641
MvaI CCWGG 3 cut(s) 273, 396, 516
MwoI GCNNNNNNNGC 2 cut(s) 659, 668
NciI CCSGG 1 cut(s) 378
NcoI CCATGG 1 cut(s) 136
NdeII GATC 3 cut(s) 203, 238, 556
NlaIII CATG 1 cut(s) 140
NlaIV GGNNCC 2 cut(s) 351, 393
NmeAIII GCCGAG 1 cut(s) 745
NmuCI GTSAC 1 cut(s) 11
PaeR7I CTCGAG 1 cut(s) 702
PciSI GCTCTTC 1 cut(s) 467
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 5 cut(s) 22, 109, 466, 779, 788
PflFI GACNNNGTC 1 cut(s) 187
PfoI TCCNGGA 2 cut(s) 376, 394
PkrI GCNGC 5 cut(s) 134, 290, 459, 619, 661
Psp6I CCWGG 3 cut(s) 271, 394, 514
PspEI GGTNACC 1 cut(s) 11
PspGI CCWGG 3 cut(s) 271, 394, 514
PspN4I GGNNCC 2 cut(s) 351, 393
PspPI GGNCC 2 cut(s) 604, 698
PspXI VCTCGAGB 1 cut(s) 702
PstI CTGCAG 1 cut(s) 664
PsuI RGATCY 1 cut(s) 238
PsyI GACNNNGTC 1 cut(s) 187
RsaI GTAC 1 cut(s) 758
RsaNI GTAC 1 cut(s) 757
Rsr2I CGGWCCG 1 cut(s) 604
RsrII CGGWCCG 1 cut(s) 604
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 5 cut(s) 303, 333, 483, 489, 510
SatI GCNGC 5 cut(s) 133, 289, 458, 618, 660
Sau3AI GATC 3 cut(s) 203, 238, 556
Sau96I GGNCC 2 cut(s) 604, 698
ScrFI CCNGG 4 cut(s) 273, 378, 396, 516
SfaNI GCATC 1 cut(s) 589
SfcI CTRYAG 1 cut(s) 660
Sfr274I CTCGAG 1 cut(s) 702
SinI GGWCC 1 cut(s) 604
SlaI CTCGAG 1 cut(s) 702
SmlI CTYRAG 2 cut(s) 302, 702
SmoI CTYRAG 2 cut(s) 302, 702
Sse9I AATT 1 cut(s) 641
SsiI CCGC 4 cut(s) 133, 162, 602, 607
SspMI CTAG 3 cut(s) 236, 246, 312
StyD4I CCNGG 4 cut(s) 271, 376, 394, 514
StyI CCWWGG 1 cut(s) 136
TaaI ACNGT 3 cut(s) 17, 128, 418
TaiI ACGT 1 cut(s) 640
TaqI TCGA 2 cut(s) 703, 777
TasI AATT 1 cut(s) 641
TauI GCSGC 1 cut(s) 135
TfiI GAWTC 5 cut(s) 22, 109, 466, 779, 788
Tru1I TTAA 5 cut(s) 303, 333, 483, 489, 510
Tru9I TTAA 5 cut(s) 303, 333, 483, 489, 510
TscAI CASTG 1 cut(s) 573
TseFI GTSAC 1 cut(s) 11
TseI GCWGC 4 cut(s) 288, 457, 617, 659
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 4 cut(s) 84, 282, 465, 663
TspGWI ACGGA 4 cut(s) 54, 168, 184, 772
TspRI CASTG 1 cut(s) 573
Tth111I GACNNNGTC 1 cut(s) 187
Vha464I CTTAAG 1 cut(s) 302
VpaK11BI GGWCC 1 cut(s) 604
XagI CCTNNNNNAGG 1 cut(s) 378
XhoI CTCGAG 1 cut(s) 702
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 3 cut(s) 236, 246, 312
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.