Rh2DG579400

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0003521
Physical Location & Seq
Forward (+)
80962670 .. 80966261
3592 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG579400.1

Sequence Viewer

Length: 705 bp
ATGGCTGTGGAGGCTGAGAAATTTGGGTCCGACGGGAAGCTTTGGAGTCTGTGCAAAAGGCCATTTTGGCAGTCAAGAAATGCTGCTCCTCCTTCTTACTCTTCATCTTCTTCTTCGTCTTCGAATTTGTCTTCTAGGCATAATGCTCAGCAGCTTAGTCGGATTCACGAGGCTGTGGAGCGTCCGACGCTTTATTCTGCCAGTACGGTGTCGTATGTGGCCAAGTCTTTGCTTCCCGCTAGGAGAAGGCTCCGTCTTGATCCTCCTAATAAGCTCTTCTTCCCATATGAACCTGGAAAGCAGGTTAAGAGTGCTGTTGGGATTAAAAACACTTCCAAGTCTCATGTAGCTTTCAAGTTCCAAACAACTGCACCAAAGAGTTGTTACATGCGTCCTCCAGGGGGAATACTTGCTCCTGGTGAAAGTCTTGTTGCAACTGTATTCAAGTTCGTGGAACCTCCGGAGAACAATGAAAAACCAGTAGACCAGAAAAGCAGGGTTAAGTTCAAGATCATGAGCTTAAAAGTGAAAGGAGAAATGGACTATGTTCCAGAATTGTTTGAGGAGCAGAAGGAACAAGTCGTTGTTGAGCAGGTTTTACGTGTTGTTTTCCTTGACCCGGAACGCCCTACCCTTGTAAGTTGGATGGAGATATCTCCAATTTGTCATTTCGCTGCACTTTGTTTATTTTATGCCTTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

234

Amino Acids

26.3

Weight (kDa)

9.47

Isoelectric Point (pI)

64.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 84 - 193 3.9e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 292, 583
AccI GTMKAC 1 cut(s) 483
AccIII TCCGGA 1 cut(s) 460
AciI CCGC 1 cut(s) 237
AclWI GGATC 1 cut(s) 254
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 2 cut(s) 20, 124
AfaI GTAC 1 cut(s) 205
AfiI CCNNNNNNNGG 2 cut(s) 401, 619
AflIII ACRYGT 1 cut(s) 601
AgsI TTSAA 3 cut(s) 355, 445, 508
AjnI CCWGG 3 cut(s) 292, 397, 415
AluBI AGCT 5 cut(s) 40, 154, 274, 350, 519
AluI AGCT 5 cut(s) 40, 154, 274, 350, 519
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 1 cut(s) 254
Aor13HI TCCGGA 1 cut(s) 460
AoxI GGCC 2 cut(s) 59, 219
ApeKI GCWGC 3 cut(s) 83, 151, 674
ApoI RAATTY 2 cut(s) 20, 124
AspS9I GGNCC 1 cut(s) 27
AsuC2I CCSGG 1 cut(s) 620
AsuHPI GGTGA 1 cut(s) 431
AsuII TTCGAA 1 cut(s) 122
AvaII GGWCC 1 cut(s) 27
BalI TGGCCA 1 cut(s) 221
BauI CACGAG 1 cut(s) 167
BbsI GAAGAC 2 cut(s) 111, 123
BbvI GCAGC 3 cut(s) 70, 163, 661
BccI CCATC 1 cut(s) 640
BcgI CGANNNNNNTGC 2 cut(s) 140, 174
BciT130I CCWGG 3 cut(s) 294, 399, 417
BcnI CCSGG 1 cut(s) 620
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 2 cut(s) 135, 240
BfuAI ACCTGC 2 cut(s) 292, 583
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 3 cut(s) 84, 152, 675
BlpI GCTNAGC 1 cut(s) 147
BlsI GCNGC 3 cut(s) 85, 153, 676
Bme1390I CCNGG 4 cut(s) 294, 399, 417, 620
Bme18I GGWCC 1 cut(s) 27
BmgT120I GGNCC 1 cut(s) 27
BmiI GGNNCC 3 cut(s) 28, 251, 456
BmrFI CCNGG 4 cut(s) 294, 399, 417, 620
BpiI GAAGAC 2 cut(s) 111, 123
BpmI CTGGAG 1 cut(s) 381
Bpu1102I GCTNAGC 1 cut(s) 147
Bpu14I TTCGAA 1 cut(s) 122
BpuMI CCSGG 1 cut(s) 620
BsaAI YACGTR 1 cut(s) 602
BsaJI CCNNGG 1 cut(s) 398
BsaWI WCCGGW 1 cut(s) 460
Bsc4I CCNNNNNNNGG 2 cut(s) 401, 619
Bse1I ACTGG 2 cut(s) 201, 479
BseAI TCCGGA 1 cut(s) 460
BseBI CCWGG 3 cut(s) 294, 399, 417
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 1 cut(s) 651
BseLI CCNNNNNNNGG 2 cut(s) 401, 619
BseMII CTCAG 2 cut(s) 6, 161
BseNI ACTGG 2 cut(s) 201, 479
BseRI GAGGAG 2 cut(s) 78, 578
BseXI GCAGC 3 cut(s) 70, 163, 661
BsgI GTGCAG 2 cut(s) 354, 660
BshFI GGCC 2 cut(s) 61, 221
BsiSI CCGG 2 cut(s) 461, 620
BslI CCNNNNNNNGG 2 cut(s) 401, 619
BsmAI GTCTC 1 cut(s) 345
BsnI GGCC 2 cut(s) 61, 221
Bsp119I TTCGAA 1 cut(s) 122
Bsp13I TCCGGA 1 cut(s) 460
Bsp143I GATC 2 cut(s) 259, 510
Bsp1720I GCTNAGC 1 cut(s) 147
BspACI CCGC 1 cut(s) 237
BspANI GGCC 2 cut(s) 61, 221
BspCNI CTCAG 2 cut(s) 7, 160
BspEI TCCGGA 1 cut(s) 460
BspHI TCATGA 1 cut(s) 513
BspLI GGNNCC 3 cut(s) 28, 251, 456
BspMI ACCTGC 2 cut(s) 292, 583
BspPI GGATC 1 cut(s) 254
BspQI GCTCTTC 1 cut(s) 281
BspT104I TTCGAA 1 cut(s) 122
BsrI ACTGG 2 cut(s) 201, 479
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 2 cut(s) 259, 510
BssSI CACGAG 1 cut(s) 167
Bst2BI CACGAG 1 cut(s) 167
Bst2UI CCWGG 3 cut(s) 294, 399, 417
Bst4CI ACNGT 2 cut(s) 208, 439
Bst6I CTCTTC 2 cut(s) 106, 281
BstBAI YACGTR 1 cut(s) 602
BstBI TTCGAA 1 cut(s) 122
BstDEI CTNAG 3 cut(s) 15, 147, 155
BstF5I GGATG 1 cut(s) 651
BstKTI GATC 2 cut(s) 262, 513
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 2 cut(s) 259, 510
BstMWI GCNNNNNNNGC 3 cut(s) 11, 67, 187
BstNI CCWGG 3 cut(s) 294, 399, 417
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 4 cut(s) 292, 397, 415, 618
BstV1I GCAGC 3 cut(s) 70, 163, 661
BstV2I GAAGAC 2 cut(s) 111, 123
BsuRI GGCC 2 cut(s) 61, 221
BtsCI GGATG 1 cut(s) 651
BveI ACCTGC 2 cut(s) 292, 583
CciI TCATGA 1 cut(s) 513
Cfr13I GGNCC 1 cut(s) 27
CseI GACGC 3 cut(s) 170, 196, 380
Csp6I GTAC 1 cut(s) 204
CviAII CATG 3 cut(s) 344, 388, 514
CviQI GTAC 1 cut(s) 204
DdeI CTNAG 3 cut(s) 15, 147, 155
DpnI GATC 2 cut(s) 261, 512
DpnII GATC 2 cut(s) 259, 510
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 106, 281
EarI CTCTTC 2 cut(s) 106, 281
Eco32I GATATC 1 cut(s) 654
Eco47I GGWCC 1 cut(s) 27
EcoRII CCWGG 3 cut(s) 292, 397, 415
EcoRV GATATC 1 cut(s) 654
FaeI CATG 3 cut(s) 347, 391, 517
FaiI YATR 9 cut(s) 141, 216, 286, 288, 345, 389, 515, 546, 693
FalI AAGNNNNNCTT 2 cut(s) 263, 295
FatI CATG 3 cut(s) 343, 387, 513
FauI CCCGC 1 cut(s) 244
FauNDI CATATG 1 cut(s) 286
FblI GTMKAC 1 cut(s) 483
Fnu4HI GCNGC 3 cut(s) 84, 152, 675
FokI GGATG 1 cut(s) 658
Fsp4HI GCNGC 3 cut(s) 84, 152, 675
FspBI CTAG 2 cut(s) 135, 240
GluI GCNGC 3 cut(s) 84, 152, 675
GsuI CTGGAG 1 cut(s) 381
HaeIII GGCC 2 cut(s) 61, 221
HapII CCGG 2 cut(s) 461, 620
HgaI GACGC 3 cut(s) 170, 196, 380
Hin1II CATG 3 cut(s) 347, 391, 517
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 2 cut(s) 46, 163
HpaII CCGG 2 cut(s) 461, 620
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 484
Hpy188I TCNGA 3 cut(s) 31, 162, 186
Hpy188III TCNNGA 7 cut(s) 75, 167, 257, 461, 508, 514, 551
Hpy8I GTNNAC 1 cut(s) 484
Hpy99I CGWCG 2 cut(s) 35, 190
HpyAV CCTTC 3 cut(s) 102, 240, 565
HpyCH4III ACNGT 2 cut(s) 208, 439
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 4 cut(s) 54, 371, 434, 677
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 67, 187
HpyF3I CTNAG 3 cut(s) 15, 147, 155
HpySE526I ACGT 1 cut(s) 601
Hsp92II CATG 3 cut(s) 347, 391, 517
Kpn2I TCCGGA 1 cut(s) 460
Kzo9I GATC 2 cut(s) 259, 510
LguI GCTCTTC 1 cut(s) 281
LmnI GCTCC 5 cut(s) 91, 178, 255, 418, 565
Lsp1109I GCAGC 3 cut(s) 70, 163, 661
MaeI CTAG 2 cut(s) 135, 240
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 1 cut(s) 383
MalI GATC 2 cut(s) 261, 512
MboI GATC 2 cut(s) 259, 510
MboII GAAGA 8 cut(s) 93, 99, 102, 105, 111, 123, 268, 271
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 4 cut(s) 20, 124, 554, 660
MluNI TGGCCA 1 cut(s) 221
MlyI GAGTC 1 cut(s) 55
MmeI TCCRAC 4 cut(s) 54, 140, 209, 623
MnlI CCTC 7 cut(s) 4, 99, 163, 273, 405, 468, 556
Mox20I TGGCCA 1 cut(s) 221
MroI TCCGGA 1 cut(s) 460
MscI TGGCCA 1 cut(s) 221
MseI TTAA 4 cut(s) 306, 324, 501, 521
Msp20I TGGCCA 1 cut(s) 221
MspI CCGG 2 cut(s) 461, 620
MspR9I CCNGG 4 cut(s) 294, 399, 417, 620
MvaI CCWGG 3 cut(s) 294, 399, 417
MwoI GCNNNNNNNGC 3 cut(s) 11, 67, 187
NciI CCSGG 1 cut(s) 620
NdeI CATATG 1 cut(s) 286
NdeII GATC 2 cut(s) 259, 510
NlaIII CATG 3 cut(s) 347, 391, 517
NlaIV GGNNCC 3 cut(s) 28, 251, 456
NspI RCATGY 1 cut(s) 391
NspV TTCGAA 1 cut(s) 122
PagI TCATGA 1 cut(s) 513
PciSI GCTCTTC 1 cut(s) 281
PfeI GAWTC 1 cut(s) 163
PkrI GCNGC 3 cut(s) 85, 153, 676
PleI GAGTC 1 cut(s) 54
PpsI GAGTC 1 cut(s) 54
Ppu21I YACGTR 1 cut(s) 602
Psp6I CCWGG 3 cut(s) 292, 397, 415
PspGI CCWGG 3 cut(s) 292, 397, 415
PspN4I GGNNCC 3 cut(s) 28, 251, 456
PspPI GGNCC 1 cut(s) 27
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
SapI GCTCTTC 1 cut(s) 281
SaqAI TTAA 4 cut(s) 306, 324, 501, 521
SatI GCNGC 3 cut(s) 84, 152, 675
Sau3AI GATC 2 cut(s) 259, 510
Sau96I GGNCC 1 cut(s) 27
SchI GAGTC 1 cut(s) 55
ScrFI CCNGG 4 cut(s) 294, 399, 417, 620
SfuI TTCGAA 1 cut(s) 122
SinI GGWCC 1 cut(s) 27
Sse9I AATT 4 cut(s) 20, 124, 554, 660
SsiI CCGC 1 cut(s) 237
SspMI CTAG 2 cut(s) 135, 240
StyD4I CCNGG 4 cut(s) 292, 397, 415, 618
TaaI ACNGT 2 cut(s) 208, 439
TaiI ACGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 122
TasI AATT 4 cut(s) 20, 124, 554, 660
TfiI GAWTC 1 cut(s) 163
Tru1I TTAA 4 cut(s) 306, 324, 501, 521
Tru9I TTAA 4 cut(s) 306, 324, 501, 521
TseI GCWGC 3 cut(s) 83, 151, 674
TspDTI ATGAA 3 cut(s) 93, 303, 486
TspGWI ACGGA 1 cut(s) 242
VpaK11BI GGWCC 1 cut(s) 27
XapI RAATTY 2 cut(s) 20, 124
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 483
XspI CTAG 2 cut(s) 135, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.