MD11G1235400.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0003521
Physical Location & Seq
Forward (+)
34262829 .. 34265462
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1235400.v1.1.491

Sequence Viewer

Length: 777 bp
ATGGCCATCGTCGACAGCTACCACCACCAGCGGCACAAGTCGGCCTCCGACACGAAGCTCTTCACCCTCTGCCCCTTCTGGCAGTCGGGAGGCCAAACGTCGTCTTCTTCTTCCTCTTCCACGCAGAAACTCCACGGCTACGGATTCAAATCCAACCCCAAAACGGTGTCCTCCGTCGCGAGATCGCTTCTCCCTCCTCGCCGCCGACTCCGGCTCGACCCCGCCAACAAACTCTACTTCCCTTATGAACCTGGGAAACAGGTGAAAAGTGCGATCCGATTGAAGAACAGTAGCAGGTCACATGTAGCTTTTAAGTTTCAAACTACTGCCCCGAAAAGCTGTTTTATGCGTCCTCCCGGAGGTATACTTGCTCCGGGAGAAAGCCTAATTGCGACCGTGTTTAGGTTTGTGGAGCAACCCGAGAAAAATGAGAAACAATTGGATCAGAAGACCAAGGTTAAGTTCAAGATTATGAGCCTCAAGGTGAACGCAGGAATCGAATATGCGCCTGAGCTGTTTGATGAACAAAAGGATCAAGTGACTGTTGAGCGAATTTTGCGGGTTGTATTTTTGAATGCAGAGCACCCTAGTCCTGCTCTGGAAAAAGTGAACAGACAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAGCCTCCTGCAGACTCAGACCCTCCTCGGGATGTTGGGGAAGGCCTTGTAATAGACGAATGGAAGGAGCGAAGGGAGAAATACTTGGCCCGGCAACAGGTTGAAGCAGTGGATTCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

259

Amino Acids

29.1

Weight (kDa)

9.65

Isoelectric Point (pI)

52.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 70 - 179 1.4e-23 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 285
AccI GTMKAC 2 cut(s) 12, 364
AccII CGCG 1 cut(s) 179
AciI CCGC 4 cut(s) 31, 202, 222, 559
AclWI GGATC 3 cut(s) 268, 450, 540
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 552
AcuI CTGAAG 1 cut(s) 645
AfiI CCNNNNNNNGG 5 cut(s) 163, 359, 402, 682, 751
AflIII ACRYGT 1 cut(s) 301
AgsI TTSAA 7 cut(s) 148, 283, 320, 466, 574, 644, 758
AjnI CCWGG 1 cut(s) 250
AjuI GAANNNNNNNTTGG 4 cut(s) 446, 478, 602, 634
AluBI AGCT 6 cut(s) 18, 58, 308, 339, 514, 629
AluI AGCT 6 cut(s) 18, 58, 308, 339, 514, 629
Alw21I GWGCWC 1 cut(s) 585
AlwI GGATC 3 cut(s) 268, 450, 540
Ama87I CYCGRG 2 cut(s) 419, 681
AoxI GGCC 5 cut(s) 3, 42, 91, 697, 741
ApeKI GCWGC 1 cut(s) 635
ApoI RAATTY 1 cut(s) 552
Asp700I GAANNNNTTC 1 cut(s) 59
AspLEI GCGC 1 cut(s) 508
AspS9I GGNCC 1 cut(s) 742
AsuC2I CCSGG 3 cut(s) 357, 375, 745
AsuHPI GGTGA 3 cut(s) 55, 274, 496
AvaI CYCGRG 2 cut(s) 419, 681
BalI TGGCCA 1 cut(s) 5
BbsI GAAGAC 2 cut(s) 96, 455
Bbv12I GWGCWC 1 cut(s) 585
BbvCI CCTCAGC 1 cut(s) 630
BbvI GCAGC 1 cut(s) 622
BccI CCATC 1 cut(s) 14
BceAI ACGGC 1 cut(s) 151
BcgI CGANNNNNNTGC 1 cut(s) 750
BciT130I CCWGG 1 cut(s) 252
BcnI CCSGG 3 cut(s) 357, 375, 745
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 1 cut(s) 663
BfuAI ACCTGC 1 cut(s) 285
BisI GCNGC 3 cut(s) 32, 202, 636
BlsI GCNGC 3 cut(s) 33, 203, 637
Bme1390I CCNGG 4 cut(s) 252, 357, 375, 745
BmeT110I CYCGRG 2 cut(s) 419, 681
BmgT120I GGNCC 1 cut(s) 742
BmrFI CCNGG 4 cut(s) 252, 357, 375, 745
BpiI GAAGAC 2 cut(s) 96, 455
Bpu10I CCTNAGC 2 cut(s) 510, 630
BpuEI CTTGAG 1 cut(s) 464
BpuMI CCSGG 3 cut(s) 357, 375, 745
BsaBI GATNNNNATC 1 cut(s) 148
BsaJI CCNNGG 4 cut(s) 133, 251, 453, 680
Bsc4I CCNNNNNNNGG 5 cut(s) 163, 359, 402, 682, 751
Bse8I GATNNNNATC 1 cut(s) 148
BseBI CCWGG 1 cut(s) 252
BseDI CCNNGG 4 cut(s) 133, 251, 453, 680
BseGI GGATG 1 cut(s) 691
BseJI GATNNNNATC 1 cut(s) 148
BseLI CCNNNNNNNGG 5 cut(s) 163, 359, 402, 682, 751
BseMII CTCAG 3 cut(s) 501, 621, 684
BseRI GAGGAG 2 cut(s) 186, 669
BseXI GCAGC 1 cut(s) 622
BsgI GTGCAG 1 cut(s) 621
Bsh1236I CGCG 1 cut(s) 179
Bsh1285I CGRYCG 1 cut(s) 396
BshFI GGCC 5 cut(s) 5, 44, 93, 699, 743
BsiEI CGRYCG 1 cut(s) 396
BsiHKAI GWGCWC 1 cut(s) 585
BsiHKCI CYCGRG 2 cut(s) 419, 681
BsiSI CCGG 4 cut(s) 211, 357, 374, 745
BslI CCNNNNNNNGG 5 cut(s) 163, 359, 402, 682, 751
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 5 cut(s) 5, 44, 93, 699, 743
BsoBI CYCGRG 2 cut(s) 419, 681
Bsp1286I GDGCHC 1 cut(s) 585
Bsp143I GATC 4 cut(s) 182, 273, 442, 532
Bsp68I TCGCGA 1 cut(s) 179
BspACI CCGC 4 cut(s) 31, 202, 222, 559
BspANI GGCC 5 cut(s) 5, 44, 93, 699, 743
BspCNI CTCAG 3 cut(s) 502, 622, 683
BspFNI CGCG 1 cut(s) 179
BspMAI CTGCAG 1 cut(s) 667
BspMI ACCTGC 1 cut(s) 285
BspPI GGATC 3 cut(s) 268, 450, 540
BspQI GCTCTTC 1 cut(s) 65
BssECI CCNNGG 4 cut(s) 133, 251, 453, 680
BssMI GATC 4 cut(s) 182, 273, 442, 532
BssNAI GTATAC 1 cut(s) 365
BssT1I CCWWGG 1 cut(s) 453
Bst1107I GTATAC 1 cut(s) 365
Bst2UI CCWGG 1 cut(s) 252
Bst4CI ACNGT 5 cut(s) 166, 290, 397, 544, 618
Bst6I CTCTTC 2 cut(s) 65, 121
BstDEI CTNAG 3 cut(s) 510, 630, 670
BstDSI CCRYGG 1 cut(s) 133
BstENI CCTNNNNNAGG 1 cut(s) 357
BstF5I GGATG 1 cut(s) 691
BstFNI CGCG 1 cut(s) 179
BstHHI GCGC 1 cut(s) 508
BstKTI GATC 4 cut(s) 185, 276, 445, 535
BstMBI GATC 4 cut(s) 182, 273, 442, 532
BstMCI CGRYCG 1 cut(s) 396
BstMWI GCNNNNNNNGC 3 cut(s) 556, 635, 655
BstNI CCWGG 1 cut(s) 252
BstNSI RCATGY 1 cut(s) 305
BstSCI CCNGG 4 cut(s) 250, 355, 373, 743
BstSFI CTRYAG 1 cut(s) 663
BstUI CGCG 1 cut(s) 179
BstV1I GCAGC 1 cut(s) 622
BstV2I GAAGAC 2 cut(s) 96, 455
BstZ17I GTATAC 1 cut(s) 365
BsuRI GGCC 5 cut(s) 5, 44, 93, 699, 743
BtgI CCRYGG 1 cut(s) 133
BtsCI GGATG 1 cut(s) 691
BtsI GCAGTG 1 cut(s) 768
BtsIMutI CAGTG 1 cut(s) 768
BtuMI TCGCGA 1 cut(s) 179
BveI ACCTGC 1 cut(s) 285
CfoI GCGC 1 cut(s) 508
Cfr13I GGNCC 1 cut(s) 742
CseI GACGC 1 cut(s) 338
CviAII CATG 1 cut(s) 302
DdeI CTNAG 3 cut(s) 510, 630, 670
DpnI GATC 4 cut(s) 184, 275, 444, 534
DpnII GATC 4 cut(s) 182, 273, 442, 532
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 65, 121
EarI CTCTTC 2 cut(s) 65, 121
Eco130I CCWWGG 1 cut(s) 453
Eco147I AGGCCT 1 cut(s) 699
Eco57I CTGAAG 1 cut(s) 645
Eco88I CYCGRG 2 cut(s) 419, 681
EcoNI CCTNNNNNAGG 1 cut(s) 357
EcoRII CCWGG 1 cut(s) 250
EcoT14I CCWWGG 1 cut(s) 453
ErhI CCWWGG 1 cut(s) 453
FaeI CATG 1 cut(s) 305
FaiI YATR 6 cut(s) 246, 303, 347, 365, 473, 504
FatI CATG 1 cut(s) 301
FauI CCCGC 2 cut(s) 229, 552
FblI GTMKAC 2 cut(s) 12, 364
Fnu4HI GCNGC 3 cut(s) 32, 202, 636
FokI GGATG 1 cut(s) 698
Fsp4HI GCNGC 3 cut(s) 32, 202, 636
FspBI CTAG 1 cut(s) 588
GlaI GCGC 1 cut(s) 507
GluI GCNGC 3 cut(s) 32, 202, 636
HaeIII GGCC 5 cut(s) 5, 44, 93, 699, 743
HapII CCGG 4 cut(s) 211, 357, 374, 745
HgaI GACGC 1 cut(s) 338
HhaI GCGC 1 cut(s) 508
Hin1II CATG 1 cut(s) 305
Hin6I GCGC 1 cut(s) 506
HinP1I GCGC 1 cut(s) 506
HincII GTYRAC 1 cut(s) 13
HindII GTYRAC 1 cut(s) 13
HinfI GANTC 5 cut(s) 144, 207, 495, 668, 767
HpaII CCGG 4 cut(s) 211, 357, 374, 745
HphI GGTGA 3 cut(s) 55, 274, 496
Hpy166II GTNNAC 4 cut(s) 13, 365, 487, 610
Hpy188I TCNGA 4 cut(s) 49, 278, 447, 673
Hpy188III TCNNGA 5 cut(s) 87, 178, 466, 599, 683
Hpy8I GTNNAC 4 cut(s) 13, 365, 487, 610
Hpy99I CGWCG 3 cut(s) 14, 103, 179
HpyAV CCTTC 4 cut(s) 85, 689, 712, 720
HpyCH4III ACNGT 5 cut(s) 166, 290, 397, 544, 618
HpyCH4IV ACGT 1 cut(s) 98
HpyCH4V TGCA 3 cut(s) 578, 638, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 556, 635, 655
HpyF3I CTNAG 3 cut(s) 510, 630, 670
HpySE526I ACGT 1 cut(s) 98
Hsp92II CATG 1 cut(s) 305
HspAI GCGC 1 cut(s) 506
Kzo9I GATC 4 cut(s) 182, 273, 442, 532
LguI GCTCTTC 1 cut(s) 65
LmnI GCTCC 3 cut(s) 376, 412, 721
Lsp1109I GCAGC 1 cut(s) 622
MaeI CTAG 1 cut(s) 588
MaeII ACGT 1 cut(s) 98
MaeIII GTNAC 2 cut(s) 297, 538
MalI GATC 4 cut(s) 184, 275, 444, 534
MboI GATC 4 cut(s) 182, 273, 442, 532
MboII GAAGA 7 cut(s) 52, 96, 99, 102, 108, 295, 460
MfeI CAATTG 1 cut(s) 437
MhlI GDGCHC 1 cut(s) 585
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 3 cut(s) 387, 437, 552
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 201, 662
MmeI TCCRAC 2 cut(s) 72, 177
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 59
MscI TGGCCA 1 cut(s) 5
MseI TTAA 2 cut(s) 312, 459
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 31
MspI CCGG 4 cut(s) 211, 357, 374, 745
MspR9I CCNGG 4 cut(s) 252, 357, 375, 745
MunI CAATTG 1 cut(s) 437
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 1 cut(s) 252
MvnI CGCG 1 cut(s) 179
MwoI GCNNNNNNNGC 3 cut(s) 556, 635, 655
NciI CCSGG 3 cut(s) 357, 375, 745
NdeII GATC 4 cut(s) 182, 273, 442, 532
NlaIII CATG 1 cut(s) 305
NmuCI GTSAC 2 cut(s) 297, 538
NruI TCGCGA 1 cut(s) 179
NspI RCATGY 1 cut(s) 305
PceI AGGCCT 1 cut(s) 699
PciI ACATGT 1 cut(s) 301
PciSI GCTCTTC 1 cut(s) 65
PcsI WCGNNNNNNNCGW 1 cut(s) 495
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 59
PfeI GAWTC 3 cut(s) 144, 495, 767
PfoI TCCNGGA 2 cut(s) 355, 373
PkrI GCNGC 3 cut(s) 33, 203, 637
PleI GAGTC 2 cut(s) 201, 662
PpsI GAGTC 2 cut(s) 201, 662
PscI ACATGT 1 cut(s) 301
Psp6I CCWGG 1 cut(s) 250
PspGI CCWGG 1 cut(s) 250
PspPI GGNCC 1 cut(s) 742
PstI CTGCAG 1 cut(s) 667
RruI TCGCGA 1 cut(s) 179
SalI GTCGAC 1 cut(s) 11
SapI GCTCTTC 1 cut(s) 65
SaqAI TTAA 2 cut(s) 312, 459
SatI GCNGC 3 cut(s) 32, 202, 636
Sau3AI GATC 4 cut(s) 182, 273, 442, 532
Sau96I GGNCC 1 cut(s) 742
SchI GAGTC 2 cut(s) 201, 662
ScrFI CCNGG 4 cut(s) 252, 357, 375, 745
SduI GDGCHC 1 cut(s) 585
SfcI CTRYAG 1 cut(s) 663
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
Sse9I AATT 3 cut(s) 387, 437, 552
SseBI AGGCCT 1 cut(s) 699
SsiI CCGC 4 cut(s) 31, 202, 222, 559
SspMI CTAG 1 cut(s) 588
StuI AGGCCT 1 cut(s) 699
StyD4I CCNGG 4 cut(s) 250, 355, 373, 743
StyI CCWWGG 1 cut(s) 453
TaaI ACNGT 5 cut(s) 166, 290, 397, 544, 618
TaiI ACGT 1 cut(s) 101
TaqI TCGA 3 cut(s) 12, 216, 498
TasI AATT 3 cut(s) 387, 437, 552
TauI GCSGC 2 cut(s) 34, 204
TfiI GAWTC 3 cut(s) 144, 495, 767
Tru1I TTAA 2 cut(s) 312, 459
Tru9I TTAA 2 cut(s) 312, 459
TscAI CASTG 1 cut(s) 768
TseFI GTSAC 2 cut(s) 297, 538
TseI GCWGC 1 cut(s) 635
Tsp45I GTSAC 2 cut(s) 297, 538
TspDTI ATGAA 2 cut(s) 261, 537
TspGWI ACGGA 2 cut(s) 156, 163
TspRI CASTG 1 cut(s) 768
XagI CCTNNNNNAGG 1 cut(s) 357
XapI RAATTY 1 cut(s) 552
XceI RCATGY 1 cut(s) 305
XmiI GTMKAC 2 cut(s) 12, 364
XmnI GAANNNNTTC 1 cut(s) 59
XspI CTAG 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.