FvH4_3g21120

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0003521
Physical Location & Seq
Reverse (-)
14143889 .. 14146340
2452 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21120.t1

Sequence Viewer

Length: 939 bp
ATGTCCCTCACACCCTGGCCACTTCACAAGTTGCAAGTTGCAACCACCACCAAATTCTGGACAGAGTTTGTTACAGAGCTCCGATTTCAATGGCCTAAAGCCGCCACGTGTCATATTCCGGGGAAGGAACTAACCATGGACCACCACCACCACCACCACCACGAATACAGCCACTCCCACCACCACCACCGCCAGCACAAGTCGCACTCCGACGGCAACCTCTTCTCTCTCTGCCCGTTCTGGCAGTCAGGTTCCCAAACGTCGTCGTCTTCATCTTCGACTCAGATCCTACAGAGCCGCGTGGACGGATCGAAATCCAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTCCTCCCGCCTCGGCGTCGGCTCCGGCTCGACCCCGCCAACAAGCTCTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCAATTCGGCTGAAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCTCCGAAAAGCTGTTACATGCGGCCTCCGGGTGGTATTCTTGCGCCGGGAGAGAGCATAATTGCGACTGTGTTTAGGTTTGTGGAACAACCTGATAGCAATGAGAAAAATGTGGATCAGAAGACCAAGGTTAAGTTCAAGATCATGAGTTTAAAGGTCAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGAGCTAGTTGCAGTTGAGCGAATTTTGCGAGTTGTATTTTTAGATGCAGAGCACCCTAGTCCTGCTTTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAACCTCCCGTAGACACAGGTCCTCGTGATGTTGGAGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATATTTGGCTCGGCAACAGGTTGAAGCAGTAGGTTCAGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

313

Amino Acids

35.56

Weight (kDa)

9.58

Isoelectric Point (pI)

50.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 125 - 232 6e-24 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 57
AccI GTMKAC 1 cut(s) 831
AccII CGCG 2 cut(s) 300, 344
AciI CCGC 6 cut(s) 102, 190, 298, 359, 387, 514
AclWI GGATC 3 cut(s) 280, 316, 615
AcoI YGGCCR 1 cut(s) 17
AcsI RAATTY 2 cut(s) 53, 717
AcuI CTGAAG 3 cut(s) 467, 794, 810
AcvI CACGTG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 367
AfiI CCNNNNNNNGG 2 cut(s) 57, 524
AflIII ACRYGT 1 cut(s) 107
AgsI TTSAA 6 cut(s) 89, 478, 485, 631, 809, 920
AjnI CCWGG 2 cut(s) 14, 415
AjuI GAANNNNNNNTTGG 4 cut(s) 611, 643, 767, 799
AluBI AGCT 8 cut(s) 79, 397, 473, 504, 656, 679, 700, 794
AluI AGCT 8 cut(s) 79, 397, 473, 504, 656, 679, 700, 794
Alw21I GWGCWC 2 cut(s) 81, 750
Alw26I GTCTC 1 cut(s) 468
AlwI GGATC 3 cut(s) 280, 316, 615
AoxI GGCC 4 cut(s) 17, 92, 515, 859
ApeKI GCWGC 1 cut(s) 800
ApoI RAATTY 2 cut(s) 53, 717
AspLEI GCGC 2 cut(s) 437, 538
AspS9I GGNCC 2 cut(s) 139, 839
AsuC2I CCSGG 3 cut(s) 120, 522, 540
AvaII GGWCC 2 cut(s) 139, 839
BalI TGGCCA 1 cut(s) 19
BanII GRGCYC 1 cut(s) 81
BauI CACGAG 1 cut(s) 843
BbrPI CACGTG 1 cut(s) 108
BbsI GAAGAC 2 cut(s) 261, 620
Bbv12I GWGCWC 2 cut(s) 81, 750
BbvCI CCTCAGC 1 cut(s) 795
BbvI GCAGC 1 cut(s) 787
BceAI ACGGC 1 cut(s) 229
BciT130I CCWGG 2 cut(s) 16, 417
BcnI CCSGG 3 cut(s) 120, 522, 540
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 2 cut(s) 701, 753
BfmI CTRYAG 1 cut(s) 290
BisI GCNGC 4 cut(s) 102, 298, 515, 801
BlsI GCNGC 4 cut(s) 103, 299, 516, 802
Bme1390I CCNGG 5 cut(s) 16, 120, 417, 522, 540
Bme18I GGWCC 2 cut(s) 139, 839
BmgT120I GGNCC 2 cut(s) 139, 839
BmiI GGNNCC 2 cut(s) 253, 374
BmrFI CCNGG 5 cut(s) 16, 120, 417, 522, 540
BmsI GCATC 1 cut(s) 730
BoxI GACNNNNGTC 1 cut(s) 837
BpiI GAAGAC 2 cut(s) 261, 620
Bpu10I CCTNAGC 2 cut(s) 675, 795
BpuMI CCSGG 3 cut(s) 120, 522, 540
BsaAI YACGTR 1 cut(s) 108
BsaBI GATNNNNATC 1 cut(s) 313
BsaHI GRCGYC 1 cut(s) 367
BsaJI CCNNGG 6 cut(s) 14, 119, 135, 362, 416, 618
BsaXI ACNNNNNCTCC 4 cut(s) 158, 188, 478, 508
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 524
Bse3DI GCAATG 1 cut(s) 598
Bse8I GATNNNNATC 1 cut(s) 313
BseBI CCWGG 2 cut(s) 16, 417
BseDI CCNNGG 6 cut(s) 14, 119, 135, 362, 416, 618
BseJI GATNNNNATC 1 cut(s) 313
BseLI CCNNNNNNNGG 2 cut(s) 57, 524
BseMI GCAATG 1 cut(s) 598
BseMII CTCAG 3 cut(s) 296, 666, 786
BseRI GAGGAG 1 cut(s) 344
BseXI GCAGC 1 cut(s) 787
BsgI GTGCAG 1 cut(s) 786
Bsh1236I CGCG 2 cut(s) 300, 344
BshFI GGCC 4 cut(s) 19, 94, 517, 861
BsiHKAI GWGCWC 2 cut(s) 81, 750
BsiSI CCGG 4 cut(s) 119, 376, 521, 539
BslI CCNNNNNNNGG 2 cut(s) 57, 524
BsmAI GTCTC 1 cut(s) 468
BsnI GGCC 4 cut(s) 19, 94, 517, 861
Bsp1286I GDGCHC 2 cut(s) 81, 750
Bsp143I GATC 5 cut(s) 285, 308, 347, 607, 633
Bsp19I CCATGG 1 cut(s) 135
Bsp68I TCGCGA 1 cut(s) 344
BspACI CCGC 6 cut(s) 102, 190, 298, 359, 387, 514
BspANI GGCC 4 cut(s) 19, 94, 517, 861
BspCNI CTCAG 3 cut(s) 295, 667, 787
BspFNI CGCG 2 cut(s) 300, 344
BspHI TCATGA 1 cut(s) 636
BspLI GGNNCC 2 cut(s) 253, 374
BspPI GGATC 3 cut(s) 280, 316, 615
BspQI GCTCTTC 1 cut(s) 425
BsrDI GCAATG 1 cut(s) 598
BssECI CCNNGG 6 cut(s) 14, 119, 135, 362, 416, 618
BssMI GATC 5 cut(s) 285, 308, 347, 607, 633
BssNI GRCGYC 1 cut(s) 367
BssSI CACGAG 1 cut(s) 843
BssT1I CCWWGG 2 cut(s) 135, 618
Bst2BI CACGAG 1 cut(s) 843
Bst2UI CCWGG 2 cut(s) 16, 417
Bst4CI ACNGT 3 cut(s) 331, 455, 562
Bst6I CTCTTC 3 cut(s) 227, 425, 770
BstACI GRCGYC 1 cut(s) 367
BstBAI YACGTR 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 194
BstDEI CTNAG 3 cut(s) 282, 675, 795
BstDSI CCRYGG 1 cut(s) 135
BstFNI CGCG 2 cut(s) 300, 344
BstHHI GCGC 2 cut(s) 437, 538
BstKTI GATC 5 cut(s) 288, 311, 350, 610, 636
BstMAI GTCTC 1 cut(s) 468
BstMBI GATC 5 cut(s) 285, 308, 347, 607, 633
BstMWI GCNNNNNNNGC 5 cut(s) 202, 358, 697, 721, 800
BstNI CCWGG 2 cut(s) 16, 417
BstNSI RCATGY 1 cut(s) 514
BstPAI GACNNNNGTC 1 cut(s) 837
BstSCI CCNGG 5 cut(s) 14, 118, 415, 520, 538
BstSFI CTRYAG 1 cut(s) 290
BstUI CGCG 2 cut(s) 300, 344
BstV1I GCAGC 1 cut(s) 787
BstV2I GAAGAC 2 cut(s) 261, 620
BstX2I RGATCY 1 cut(s) 285
BstYI RGATCY 1 cut(s) 285
BsuRI GGCC 4 cut(s) 19, 94, 517, 861
BtgI CCRYGG 1 cut(s) 135
BtuMI TCGCGA 1 cut(s) 344
Cac8I GCNNGC 1 cut(s) 194
CciI TCATGA 1 cut(s) 636
CfoI GCGC 2 cut(s) 437, 538
Cfr13I GGNCC 2 cut(s) 139, 839
CseI GACGC 1 cut(s) 356
CviAII CATG 4 cut(s) 136, 467, 511, 637
DdeI CTNAG 3 cut(s) 282, 675, 795
DpnI GATC 5 cut(s) 287, 310, 349, 609, 635
DpnII GATC 5 cut(s) 285, 308, 347, 607, 633
DraI TTTAAA 1 cut(s) 645
EaeI YGGCCR 1 cut(s) 17
Eam1104I CTCTTC 3 cut(s) 227, 425, 770
EarI CTCTTC 3 cut(s) 227, 425, 770
Ecl136II GAGCTC 1 cut(s) 79
Eco130I CCWWGG 2 cut(s) 135, 618
Eco147I AGGCCT 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 81
Eco47I GGWCC 2 cut(s) 139, 839
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 3 cut(s) 467, 794, 810
Eco72I CACGTG 1 cut(s) 108
EcoICRI GAGCTC 1 cut(s) 79
EcoO109I RGGNCCY 1 cut(s) 839
EcoRII CCWGG 2 cut(s) 14, 415
EcoT14I CCWWGG 2 cut(s) 135, 618
EcoT38I GRGCYC 1 cut(s) 81
ErhI CCWWGG 2 cut(s) 135, 618
FaeI CATG 4 cut(s) 139, 470, 514, 640
FaiI YATR 8 cut(s) 114, 137, 411, 468, 512, 551, 638, 669
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FatI CATG 4 cut(s) 135, 466, 510, 636
FauI CCCGC 2 cut(s) 366, 394
FblI GTMKAC 1 cut(s) 831
Fnu4HI GCNGC 4 cut(s) 102, 298, 515, 801
FriOI GRGCYC 1 cut(s) 81
Fsp4HI GCNGC 4 cut(s) 102, 298, 515, 801
FspBI CTAG 2 cut(s) 701, 753
GlaI GCGC 2 cut(s) 436, 537
GluI GCNGC 4 cut(s) 102, 298, 515, 801
HaeIII GGCC 4 cut(s) 19, 94, 517, 861
HapII CCGG 4 cut(s) 119, 376, 521, 539
HgaI GACGC 1 cut(s) 356
HhaI GCGC 2 cut(s) 437, 538
Hin1I GRCGYC 1 cut(s) 367
Hin1II CATG 4 cut(s) 139, 470, 514, 640
Hin6I GCGC 2 cut(s) 435, 536
HinP1I GCGC 2 cut(s) 435, 536
HinfI GANTC 1 cut(s) 280
HpaII CCGG 4 cut(s) 119, 376, 521, 539
Hpy166II GTNNAC 2 cut(s) 304, 832
Hpy188I TCNGA 5 cut(s) 83, 211, 285, 498, 612
Hpy188III TCNNGA 6 cut(s) 58, 343, 631, 637, 674, 845
Hpy8I GTNNAC 2 cut(s) 304, 832
Hpy99I CGWCG 5 cut(s) 215, 265, 268, 344, 372
HpyAV CCTTC 4 cut(s) 118, 851, 874, 882
HpyCH4III ACNGT 3 cut(s) 331, 455, 562
HpyCH4IV ACGT 2 cut(s) 107, 260
HpyCH4V TGCA 5 cut(s) 34, 41, 707, 743, 803
HpyF10VI GCNNNNNNNGC 5 cut(s) 202, 358, 697, 721, 800
HpyF3I CTNAG 3 cut(s) 282, 675, 795
HpySE526I ACGT 2 cut(s) 107, 260
Hsp92I GRCGYC 1 cut(s) 367
Hsp92II CATG 4 cut(s) 139, 470, 514, 640
HspAI GCGC 2 cut(s) 435, 536
Kzo9I GATC 5 cut(s) 285, 308, 347, 607, 633
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 7 cut(s) 84, 357, 378, 499, 676, 697, 883
Lsp1109I GCAGC 1 cut(s) 787
LweI GCATC 1 cut(s) 730
MaeI CTAG 2 cut(s) 701, 753
MaeII ACGT 2 cut(s) 107, 260
MaeIII GTNAC 2 cut(s) 70, 506
MalI GATC 5 cut(s) 287, 310, 349, 609, 635
MboI GATC 5 cut(s) 285, 308, 347, 607, 633
MboII GAAGA 7 cut(s) 214, 261, 267, 442, 460, 625, 787
MflI RGATCY 1 cut(s) 285
MhlI GDGCHC 2 cut(s) 81, 750
MlsI TGGCCA 1 cut(s) 19
MluCI AATT 4 cut(s) 53, 438, 552, 717
MluNI TGGCCA 1 cut(s) 19
MlyI GAGTC 1 cut(s) 274
MmeI TCCRAC 2 cut(s) 234, 832
MnlI CCTC 9 cut(s) 17, 230, 365, 372, 528, 771, 790, 834, 852
Mox20I TGGCCA 1 cut(s) 19
MscI TGGCCA 1 cut(s) 19
MseI TTAA 3 cut(s) 624, 644, 937
Msp20I TGGCCA 1 cut(s) 19
MspI CCGG 4 cut(s) 119, 376, 521, 539
MspR9I CCNGG 5 cut(s) 16, 120, 417, 522, 540
MvaI CCWGG 2 cut(s) 16, 417
MvnI CGCG 2 cut(s) 300, 344
MwoI GCNNNNNNNGC 5 cut(s) 202, 358, 697, 721, 800
NciI CCSGG 3 cut(s) 120, 522, 540
NcoI CCATGG 1 cut(s) 135
NdeII GATC 5 cut(s) 285, 308, 347, 607, 633
NlaIII CATG 4 cut(s) 139, 470, 514, 640
NlaIV GGNNCC 2 cut(s) 253, 374
NmeAIII GCCGAG 2 cut(s) 343, 886
NruI TCGCGA 1 cut(s) 344
NspI RCATGY 1 cut(s) 514
PagI TCATGA 1 cut(s) 636
PceI AGGCCT 1 cut(s) 861
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 341
PflMI CCANNNNNTGG 1 cut(s) 57
PkrI GCNGC 4 cut(s) 103, 299, 516, 802
PleI GAGTC 1 cut(s) 274
PmaCI CACGTG 1 cut(s) 108
PmlI CACGTG 1 cut(s) 108
PpsI GAGTC 1 cut(s) 274
Ppu21I YACGTR 1 cut(s) 108
PpuMI RGGWCCY 1 cut(s) 839
PshAI GACNNNNGTC 1 cut(s) 837
Psp124BI GAGCTC 1 cut(s) 81
Psp5II RGGWCCY 1 cut(s) 839
Psp6I CCWGG 2 cut(s) 14, 415
PspCI CACGTG 1 cut(s) 108
PspGI CCWGG 2 cut(s) 14, 415
PspN4I GGNNCC 2 cut(s) 253, 374
PspPI GGNCC 2 cut(s) 139, 839
PspPPI RGGWCCY 1 cut(s) 839
PsuI RGATCY 1 cut(s) 285
RruI TCGCGA 1 cut(s) 344
SacI GAGCTC 1 cut(s) 81
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 3 cut(s) 624, 644, 937
SatI GCNGC 4 cut(s) 102, 298, 515, 801
Sau3AI GATC 5 cut(s) 285, 308, 347, 607, 633
Sau96I GGNCC 2 cut(s) 139, 839
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 5 cut(s) 16, 120, 417, 522, 540
SduI GDGCHC 2 cut(s) 81, 750
SfaNI GCATC 1 cut(s) 730
SfcI CTRYAG 1 cut(s) 290
SinI GGWCC 2 cut(s) 139, 839
Sse9I AATT 4 cut(s) 53, 438, 552, 717
SseBI AGGCCT 1 cut(s) 861
SsiI CCGC 6 cut(s) 102, 190, 298, 359, 387, 514
SspI AATATT 1 cut(s) 899
SspMI CTAG 2 cut(s) 701, 753
SstI GAGCTC 1 cut(s) 81
StuI AGGCCT 1 cut(s) 861
StyD4I CCNGG 5 cut(s) 14, 118, 415, 520, 538
StyI CCWWGG 2 cut(s) 135, 618
TaaI ACNGT 3 cut(s) 331, 455, 562
TaiI ACGT 2 cut(s) 110, 263
TaqI TCGA 3 cut(s) 278, 311, 381
TasI AATT 4 cut(s) 53, 438, 552, 717
TauI GCSGC 3 cut(s) 104, 300, 517
Tru1I TTAA 3 cut(s) 624, 644, 937
Tru9I TTAA 3 cut(s) 624, 644, 937
TseI GCWGC 1 cut(s) 800
TspDTI ATGAA 3 cut(s) 261, 426, 888
TspGWI ACGGA 3 cut(s) 321, 328, 677
Van91I CCANNNNNTGG 1 cut(s) 57
VpaK11BI GGWCC 2 cut(s) 139, 839
XapI RAATTY 2 cut(s) 53, 717
XceI RCATGY 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 831
XspI CTAG 2 cut(s) 701, 753
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.