RchiOBHm_Chr5g0036131

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0003521
Physical Location & Seq
Reverse (-)
30245853 .. 30248162
2310 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31495

Sequence Viewer

Length: 810 bp
ATGGACCACCACGACTACAGCCACTCCCACCACCACCACCACAGCCAGAGCAGCAGCCACCACCACCACCGGCACAAGTCCCACTCCGACGGCAACCTCTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGTGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTGCTCCCGCCTCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTCTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCGATTCGGTTGAAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCTGACAGCAATGAGAAAAATGTGGATCAGAAGGCCAAGGTTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGATCAAGTAGCAGTTGAGCGAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTCTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAACCTCCCGCAGATTCAGGTCCTCGTGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTCGAAGCAGTAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

269

Amino Acids

30.27

Weight (kDa)

9.5

Isoelectric Point (pI)

51.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 84 - 194 1.1e-24 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 243
AccI GTMKAC 1 cut(s) 180
AccII CGCG 2 cut(s) 221, 314
AciI CCGC 3 cut(s) 236, 601, 705
AclWI GGATC 4 cut(s) 157, 193, 492, 582
AcsI RAATTY 1 cut(s) 594
AcuI CTGAAG 3 cut(s) 542, 671, 687
AcyI GRCGYC 2 cut(s) 177, 244
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 5 cut(s) 325, 355, 362, 508, 686
AjnI CCWGG 3 cut(s) 292, 415, 777
AjuI GAANNNNNNNTTGG 4 cut(s) 488, 520, 644, 676
AluBI AGCT 5 cut(s) 350, 381, 533, 556, 671
AluI AGCT 5 cut(s) 350, 381, 533, 556, 671
Alw21I GWGCWC 1 cut(s) 627
Alw26I GTCTC 1 cut(s) 345
AlwI GGATC 4 cut(s) 157, 193, 492, 582
AoxI GGCC 2 cut(s) 492, 736
ApeKI GCWGC 4 cut(s) 51, 54, 229, 677
ApoI RAATTY 1 cut(s) 594
AspLEI GCGC 2 cut(s) 246, 314
AspS9I GGNCC 3 cut(s) 4, 129, 716
AsuC2I CCSGG 1 cut(s) 399
AsuHPI GGTGA 1 cut(s) 538
AvaII GGWCC 3 cut(s) 4, 129, 716
BanI GGYRCC 1 cut(s) 243
BauI CACGAG 1 cut(s) 720
BbsI GAAGAC 1 cut(s) 138
Bbv12I GWGCWC 1 cut(s) 627
BbvCI CCTCAGC 1 cut(s) 672
BbvI GCAGC 4 cut(s) 63, 66, 216, 664
BceAI ACGGC 1 cut(s) 106
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 3 cut(s) 294, 417, 779
BcnI CCSGG 1 cut(s) 399
BcoDI GTCTC 1 cut(s) 345
BfaI CTAG 1 cut(s) 641
BfmI CTRYAG 2 cut(s) 16, 167
BfoI RGCGCY 1 cut(s) 247
BisI GCNGC 4 cut(s) 52, 55, 230, 678
BlsI GCNGC 4 cut(s) 53, 56, 231, 679
Bme1390I CCNGG 4 cut(s) 294, 399, 417, 779
Bme18I GGWCC 3 cut(s) 4, 129, 716
BmgT120I GGNCC 3 cut(s) 4, 129, 716
BmiI GGNNCC 4 cut(s) 130, 131, 245, 251
BmrFI CCNGG 4 cut(s) 294, 399, 417, 779
BmsI GCATC 1 cut(s) 607
BpiI GAAGAC 1 cut(s) 138
BpmI CTGGAG 1 cut(s) 399
Bpu10I CCTNAGC 2 cut(s) 552, 672
BpuMI CCSGG 1 cut(s) 399
BsaHI GRCGYC 2 cut(s) 177, 244
BsaJI CCNNGG 3 cut(s) 239, 293, 495
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse118I RCCGGY 2 cut(s) 69, 246
Bse3DI GCAATG 1 cut(s) 475
BseBI CCWGG 3 cut(s) 294, 417, 779
BseDI CCNNGG 3 cut(s) 239, 293, 495
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 475
BseMII CTCAG 3 cut(s) 173, 543, 663
BseXI GCAGC 4 cut(s) 63, 66, 216, 664
BsgI GTGCAG 2 cut(s) 354, 663
Bsh1236I CGCG 2 cut(s) 221, 314
BshFI GGCC 2 cut(s) 494, 738
BshNI GGYRCC 1 cut(s) 243
BsiHKAI GWGCWC 1 cut(s) 627
BsiSI CCGG 4 cut(s) 70, 247, 253, 398
BslFI GGGAC 2 cut(s) 64, 115
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 345
BsmFI GGGAC 2 cut(s) 64, 115
BsnI GGCC 2 cut(s) 494, 738
Bsp1286I GDGCHC 1 cut(s) 627
Bsp143I GATC 5 cut(s) 162, 185, 224, 484, 574
Bsp68I TCGCGA 1 cut(s) 221
BspACI CCGC 3 cut(s) 236, 601, 705
BspANI GGCC 2 cut(s) 494, 738
BspCNI CTCAG 3 cut(s) 172, 544, 664
BspFNI CGCG 2 cut(s) 221, 314
BspLI GGNNCC 4 cut(s) 130, 131, 245, 251
BspPI GGATC 4 cut(s) 157, 193, 492, 582
BspQI GCTCTTC 1 cut(s) 302
BspT107I GGYRCC 1 cut(s) 243
BsrDI GCAATG 1 cut(s) 475
BsrFI RCCGGY 2 cut(s) 69, 246
BssAI RCCGGY 2 cut(s) 69, 246
BssECI CCNNGG 3 cut(s) 239, 293, 495
BssMI GATC 5 cut(s) 162, 185, 224, 484, 574
BssNI GRCGYC 2 cut(s) 177, 244
BssSI CACGAG 1 cut(s) 720
BssT1I CCWWGG 1 cut(s) 495
Bst2BI CACGAG 1 cut(s) 720
Bst2UI CCWGG 3 cut(s) 294, 417, 779
Bst4CI ACNGT 3 cut(s) 208, 332, 439
Bst6I CTCTTC 4 cut(s) 104, 157, 302, 647
BstACI GRCGYC 2 cut(s) 177, 244
BstC8I GCNNGC 2 cut(s) 248, 268
BstDEI CTNAG 3 cut(s) 159, 552, 672
BstFNI CGCG 2 cut(s) 221, 314
BstH2I RGCGCY 1 cut(s) 247
BstHHI GCGC 2 cut(s) 246, 314
BstKTI GATC 5 cut(s) 165, 188, 227, 487, 577
BstMAI GTCTC 1 cut(s) 345
BstMBI GATC 5 cut(s) 162, 185, 224, 484, 574
BstMWI GCNNNNNNNGC 4 cut(s) 51, 235, 598, 677
BstNI CCWGG 3 cut(s) 294, 417, 779
BstNSI RCATGY 1 cut(s) 391
BstSCI CCNGG 4 cut(s) 292, 397, 415, 777
BstSFI CTRYAG 2 cut(s) 16, 167
BstUI CGCG 2 cut(s) 221, 314
BstV1I GCAGC 4 cut(s) 63, 66, 216, 664
BstV2I GAAGAC 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BsuRI GGCC 2 cut(s) 494, 738
BtuMI TCGCGA 1 cut(s) 221
Cac8I GCNNGC 2 cut(s) 248, 268
CfoI GCGC 2 cut(s) 246, 314
Cfr10I RCCGGY 2 cut(s) 69, 246
Cfr13I GGNCC 3 cut(s) 4, 129, 716
CseI GACGC 2 cut(s) 166, 380
CviAII CATG 2 cut(s) 344, 388
DdeI CTNAG 3 cut(s) 159, 552, 672
DinI GGCGCC 1 cut(s) 245
DpnI GATC 5 cut(s) 164, 187, 226, 486, 576
DpnII GATC 5 cut(s) 162, 185, 224, 484, 574
Eam1104I CTCTTC 4 cut(s) 104, 157, 302, 647
EarI CTCTTC 4 cut(s) 104, 157, 302, 647
Eco130I CCWWGG 1 cut(s) 495
Eco147I AGGCCT 1 cut(s) 738
Eco47I GGWCC 3 cut(s) 4, 129, 716
Eco57I CTGAAG 3 cut(s) 542, 671, 687
EcoO109I RGGNCCY 2 cut(s) 129, 716
EcoRII CCWGG 3 cut(s) 292, 415, 777
EcoT14I CCWWGG 1 cut(s) 495
EgeI GGCGCC 1 cut(s) 245
EheI GGCGCC 1 cut(s) 245
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 2 cut(s) 347, 391
FaiI YATR 6 cut(s) 288, 345, 389, 428, 515, 546
FaqI GGGAC 2 cut(s) 64, 115
FatI CATG 2 cut(s) 343, 387
FauI CCCGC 3 cut(s) 243, 594, 712
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 4 cut(s) 52, 55, 230, 678
Fsp4HI GCNGC 4 cut(s) 52, 55, 230, 678
FspBI CTAG 1 cut(s) 641
GlaI GCGC 2 cut(s) 245, 313
GluI GCNGC 4 cut(s) 52, 55, 230, 678
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 247
HaeIII GGCC 2 cut(s) 494, 738
HapII CCGG 4 cut(s) 70, 247, 253, 398
HgaI GACGC 2 cut(s) 166, 380
HhaI GCGC 2 cut(s) 246, 314
Hin1I GRCGYC 2 cut(s) 177, 244
Hin1II CATG 2 cut(s) 347, 391
Hin6I GCGC 2 cut(s) 244, 312
HinP1I GCGC 2 cut(s) 244, 312
HincII GTYRAC 1 cut(s) 181
HindII GTYRAC 1 cut(s) 181
HinfI GANTC 4 cut(s) 157, 316, 517, 710
HpaII CCGG 4 cut(s) 70, 247, 253, 398
HphI GGTGA 1 cut(s) 538
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 4 cut(s) 88, 162, 489, 522
Hpy188III TCNNGA 4 cut(s) 220, 551, 641, 722
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 6 cut(s) 92, 142, 145, 182, 185, 221
HpyAV CCTTC 5 cut(s) 484, 517, 728, 751, 759
HpyCH4III ACNGT 3 cut(s) 208, 332, 439
HpyCH4IV ACGT 1 cut(s) 137
HpyCH4V TGCA 3 cut(s) 371, 620, 680
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 235, 598, 677
HpyF3I CTNAG 3 cut(s) 159, 552, 672
HpySE526I ACGT 1 cut(s) 137
Hsp92I GRCGYC 2 cut(s) 177, 244
Hsp92II CATG 2 cut(s) 347, 391
HspAI GCGC 2 cut(s) 244, 312
KasI GGCGCC 1 cut(s) 243
KflI GGGWCCC 1 cut(s) 129
KroI GCCGGC 1 cut(s) 246
KroNI GCCGGC 1 cut(s) 248
Kzo9I GATC 5 cut(s) 162, 185, 224, 484, 574
LguI GCTCTTC 1 cut(s) 302
LmnI GCTCC 5 cut(s) 237, 255, 418, 553, 760
Lsp1109I GCAGC 4 cut(s) 63, 66, 216, 664
LweI GCATC 1 cut(s) 607
MaeI CTAG 1 cut(s) 641
MaeII ACGT 1 cut(s) 137
MaeIII GTNAC 1 cut(s) 383
MalI GATC 5 cut(s) 164, 187, 226, 486, 576
MboI GATC 5 cut(s) 162, 185, 224, 484, 574
MboII GAAGA 6 cut(s) 91, 138, 144, 319, 337, 664
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 627
MluCI AATT 3 cut(s) 429, 510, 594
Mly113I GGCGCC 1 cut(s) 244
MlyI GAGTC 2 cut(s) 151, 526
MmeI TCCRAC 1 cut(s) 111
MnlI CCTC 8 cut(s) 107, 160, 249, 405, 648, 667, 711, 729
MroNI GCCGGC 1 cut(s) 246
MseI TTAA 1 cut(s) 501
MspI CCGG 4 cut(s) 70, 247, 253, 398
MspR9I CCNGG 4 cut(s) 294, 399, 417, 779
MvaI CCWGG 3 cut(s) 294, 417, 779
MvnI CGCG 2 cut(s) 221, 314
MwoI GCNNNNNNNGC 4 cut(s) 51, 235, 598, 677
NaeI GCCGGC 1 cut(s) 248
NarI GGCGCC 1 cut(s) 244
NciI CCSGG 1 cut(s) 399
NdeII GATC 5 cut(s) 162, 185, 224, 484, 574
NgoMIV GCCGGC 1 cut(s) 246
NlaIII CATG 2 cut(s) 347, 391
NlaIV GGNNCC 4 cut(s) 130, 131, 245, 251
NmeAIII GCCGAG 2 cut(s) 220, 763
NruI TCGCGA 1 cut(s) 221
NspI RCATGY 1 cut(s) 391
PceI AGGCCT 1 cut(s) 738
PciSI GCTCTTC 1 cut(s) 302
PcsI WCGNNNNNNNCGW 1 cut(s) 218
PdiI GCCGGC 1 cut(s) 248
PfeI GAWTC 2 cut(s) 316, 710
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 4 cut(s) 53, 56, 231, 679
PleI GAGTC 2 cut(s) 151, 525
PluTI GGCGCC 1 cut(s) 247
PpsI GAGTC 2 cut(s) 151, 525
PpuMI RGGWCCY 2 cut(s) 129, 716
Psp5II RGGWCCY 2 cut(s) 129, 716
Psp6I CCWGG 3 cut(s) 292, 415, 777
PspGI CCWGG 3 cut(s) 292, 415, 777
PspN4I GGNNCC 4 cut(s) 130, 131, 245, 251
PspPI GGNCC 3 cut(s) 4, 129, 716
PspPPI RGGWCCY 2 cut(s) 129, 716
PsuI RGATCY 1 cut(s) 162
RruI TCGCGA 1 cut(s) 221
SalI GTCGAC 1 cut(s) 179
SapI GCTCTTC 1 cut(s) 302
SaqAI TTAA 1 cut(s) 501
SatI GCNGC 4 cut(s) 52, 55, 230, 678
Sau3AI GATC 5 cut(s) 162, 185, 224, 484, 574
Sau96I GGNCC 3 cut(s) 4, 129, 716
SchI GAGTC 2 cut(s) 151, 526
ScrFI CCNGG 4 cut(s) 294, 399, 417, 779
SduI GDGCHC 1 cut(s) 627
SfaNI GCATC 1 cut(s) 607
SfcI CTRYAG 2 cut(s) 16, 167
SfoI GGCGCC 1 cut(s) 245
SgrDI CGTCGACG 1 cut(s) 179
SinI GGWCC 3 cut(s) 4, 129, 716
Sse9I AATT 3 cut(s) 429, 510, 594
SseBI AGGCCT 1 cut(s) 738
SsiI CCGC 3 cut(s) 236, 601, 705
SspDI GGCGCC 1 cut(s) 243
SspMI CTAG 1 cut(s) 641
StuI AGGCCT 1 cut(s) 738
StyD4I CCNGG 4 cut(s) 292, 397, 415, 777
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 3 cut(s) 208, 332, 439
TaiI ACGT 1 cut(s) 140
TaqI TCGA 6 cut(s) 155, 180, 188, 194, 258, 795
TasI AATT 3 cut(s) 429, 510, 594
TfiI GAWTC 2 cut(s) 316, 710
Tru1I TTAA 1 cut(s) 501
Tru9I TTAA 1 cut(s) 501
TseI GCWGC 4 cut(s) 51, 54, 229, 677
TspDTI ATGAA 2 cut(s) 303, 765
TspGWI ACGGA 4 cut(s) 102, 198, 205, 554
VpaK11BI GGWCC 3 cut(s) 4, 129, 716
XapI RAATTY 1 cut(s) 594
XbaI TCTAGA 1 cut(s) 640
XceI RCATGY 1 cut(s) 391
XmiI GTMKAC 1 cut(s) 180
XspI CTAG 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.