MD17G1085000.v1.1

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0003521
Physical Location & Seq
Reverse (-)
6996830 .. 7000027
3198 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1085000.v1.1.491

Sequence Viewer

Length: 807 bp
ATGGCTGTGGAGGCTGAGAAATCTGGGCCTGACGGGAAGCTTTGGAGTCTATTCAAAATGCCGTTTTGGCAGTCCGGGAACGCTTCTTCGTCGTCGTCCTCGACCTTGTCTTCAATGCATCACAATGTTCACCACCAGCTGGGTCAGATTCACGAGTCTGCGGCGGAGCGGATGGCTCTTCATTCCGCCAGTACGGTGTCGTACGTGACCAAGTCTTTGCTTCCGGCTAGGAGAAGGCTGCGTCTTGATCCTCCGAATAAGCTCTTTTTTCCATATGAACCCGGTAAGCAGGTCAGGAGTGCAATTGGGATAAAAAACACAAGCAAGTCTCTTGTAGCTTTCAAGTTCCAAACAACTGCACCAAAGAGTTGTTACATGCGTCCTCCTGGAGGTATACTTGCTCCTGGTGAAAGTCTTATTGCAACTGTTTTCAAGTTTGTGGAACCTCCGGAGAACGATGAGAAACCAGCTGTAGACCAGAAGAGCAGGGTGAAGTTCAAGATCATGAGCTTAAAAGTGAAAGGAGATATGGACTATGTACCAGAACTGTTTGAGGAGCAGAGGGACGAAGTTGTGGTTGAGCAGGTTTTGCGTGTTGTTTTCCTTGACCCTGAGCACCCTACCCCTGCAATGGAAAAACTTAAGCGGCAATTGGCTGAAGCTGAAGCTGCCCTTGAAACACGAAAGAAGCCTCCAGAAGAAGCAGGCCCCCGGATTGTAGGGGAAGGACTTGTCATAGATGAATGGAAAGAGCGCAGGGAAAGATACCTAGCGCAGCAGCAGGTTGAAGGGGCTGATTCAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

269

Amino Acids

30.04

Weight (kDa)

8.49

Isoelectric Point (pI)

51.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 80 - 188 9.6e-22 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 280, 574, 772
AccB7I CCANNNNNTGG 1 cut(s) 139
AccBSI CCGCTC 1 cut(s) 169
AccI GTMKAC 2 cut(s) 394, 474
AccIII TCCGGA 1 cut(s) 448
AciI CCGC 5 cut(s) 161, 164, 169, 186, 646
AclWI GGATC 1 cut(s) 242
AcuI CTGAAG 2 cut(s) 678, 684
AfaI GTAC 3 cut(s) 193, 203, 540
AfiI CCNNNNNNNGG 3 cut(s) 139, 389, 631
AflII CTTAAG 1 cut(s) 641
AgsI TTSAA 7 cut(s) 55, 114, 343, 433, 499, 677, 788
AjnI CCWGG 2 cut(s) 385, 403
AluBI AGCT 8 cut(s) 40, 139, 262, 338, 470, 510, 662, 668
AluI AGCT 8 cut(s) 40, 139, 262, 338, 470, 510, 662, 668
Alw21I GWGCWC 1 cut(s) 618
Alw26I GTCTC 1 cut(s) 333
AlwI GGATC 1 cut(s) 242
Aor13HI TCCGGA 1 cut(s) 448
AoxI GGCC 2 cut(s) 26, 706
ApeKI GCWGC 4 cut(s) 238, 668, 775, 778
ArsI GACNNNNNNTTYG 2 cut(s) 199, 231
AspLEI GCGC 2 cut(s) 756, 775
AspS9I GGNCC 2 cut(s) 26, 707
AsuC2I CCSGG 3 cut(s) 76, 282, 712
AsuHPI GGTGA 3 cut(s) 122, 419, 502
BauI CACGAG 1 cut(s) 152
BbsI GAAGAC 1 cut(s) 102
Bbv12I GWGCWC 1 cut(s) 618
BbvI GCAGC 4 cut(s) 225, 655, 787, 790
BccI CCATC 1 cut(s) 166
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 2 cut(s) 387, 405
BcnI CCSGG 3 cut(s) 76, 282, 712
BcoDI GTCTC 1 cut(s) 333
BfaI CTAG 2 cut(s) 228, 770
BfmI CTRYAG 1 cut(s) 471
BfrI CTTAAG 1 cut(s) 641
BfuAI ACCTGC 3 cut(s) 280, 574, 772
BglI GCCNNNNNGGC 1 cut(s) 67
BisI GCNGC 6 cut(s) 162, 239, 647, 669, 776, 779
BlsI GCNGC 6 cut(s) 163, 240, 648, 670, 777, 780
Bme1390I CCNGG 5 cut(s) 76, 282, 387, 405, 712
BmgT120I GGNCC 2 cut(s) 26, 707
BmiI GGNNCC 2 cut(s) 444, 709
BmrFI CCNGG 5 cut(s) 76, 282, 387, 405, 712
BmsI GCATC 1 cut(s) 127
BpiI GAAGAC 1 cut(s) 102
BpmI CTGGAG 2 cut(s) 408, 678
Bpu10I CCTNAGC 1 cut(s) 612
BpuMI CCSGG 3 cut(s) 76, 282, 712
BsaAI YACGTR 1 cut(s) 205
BsaJI CCNNGG 1 cut(s) 710
BsaWI WCCGGW 1 cut(s) 448
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 389, 631
Bse1I ACTGG 1 cut(s) 189
Bse3DI GCAATG 1 cut(s) 636
BseAI TCCGGA 1 cut(s) 448
BseBI CCWGG 2 cut(s) 387, 405
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 1 cut(s) 177
BseLI CCNNNNNNNGG 3 cut(s) 139, 389, 631
BseMI GCAATG 1 cut(s) 636
BseMII CTCAG 2 cut(s) 6, 603
BseNI ACTGG 1 cut(s) 189
BseRI GAGGAG 1 cut(s) 569
BseXI GCAGC 4 cut(s) 225, 655, 787, 790
BseYI CCCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 342
BshFI GGCC 2 cut(s) 28, 708
BsiHKAI GWGCWC 1 cut(s) 618
BsiSI CCGG 5 cut(s) 75, 224, 282, 449, 712
BsiWI CGTACG 1 cut(s) 201
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 3 cut(s) 139, 389, 631
BsmAI GTCTC 1 cut(s) 333
BsmFI GGGAC 1 cut(s) 578
BsnI GGCC 2 cut(s) 28, 708
Bsp1286I GDGCHC 1 cut(s) 618
Bsp13I TCCGGA 1 cut(s) 448
Bsp143I GATC 2 cut(s) 247, 501
BspACI CCGC 5 cut(s) 161, 164, 169, 186, 646
BspANI GGCC 2 cut(s) 28, 708
BspCNI CTCAG 2 cut(s) 7, 604
BspEI TCCGGA 1 cut(s) 448
BspHI TCATGA 1 cut(s) 504
BspLI GGNNCC 2 cut(s) 444, 709
BspMI ACCTGC 3 cut(s) 280, 574, 772
BspPI GGATC 1 cut(s) 242
BspQI GCTCTTC 2 cut(s) 183, 476
BspTI CTTAAG 1 cut(s) 641
BsrBI CCGCTC 1 cut(s) 169
BsrDI GCAATG 1 cut(s) 636
BsrI ACTGG 1 cut(s) 189
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 2 cut(s) 247, 501
BssNAI GTATAC 1 cut(s) 395
BssSI CACGAG 1 cut(s) 152
Bst1107I GTATAC 1 cut(s) 395
Bst2BI CACGAG 1 cut(s) 152
Bst2UI CCWGG 2 cut(s) 387, 405
Bst4CI ACNGT 3 cut(s) 196, 427, 549
Bst6I CTCTTC 2 cut(s) 183, 476
BstAFI CTTAAG 1 cut(s) 641
BstAPI GCANNNNNTGC 1 cut(s) 589
BstBAI YACGTR 1 cut(s) 205
BstC8I GCNNGC 1 cut(s) 706
BstDEI CTNAG 2 cut(s) 15, 612
BstENI CCTNNNNNAGG 1 cut(s) 387
BstF5I GGATG 1 cut(s) 177
BstHHI GCGC 2 cut(s) 756, 775
BstKTI GATC 2 cut(s) 250, 504
BstMAI GTCTC 1 cut(s) 333
BstMBI GATC 2 cut(s) 247, 501
BstMWI GCNNNNNNNGC 4 cut(s) 11, 67, 589, 668
BstNI CCWGG 2 cut(s) 387, 405
BstNSI RCATGY 1 cut(s) 379
BstSCI CCNGG 5 cut(s) 74, 280, 385, 403, 710
BstSFI CTRYAG 1 cut(s) 471
BstV1I GCAGC 4 cut(s) 225, 655, 787, 790
BstV2I GAAGAC 1 cut(s) 102
BstZ17I GTATAC 1 cut(s) 395
BsuRI GGCC 2 cut(s) 28, 708
BtsCI GGATG 1 cut(s) 177
BtsIMutI CAGTG 1 cut(s) 807
BveI ACCTGC 3 cut(s) 280, 574, 772
Cac8I GCNNGC 1 cut(s) 706
CciI TCATGA 1 cut(s) 504
CfoI GCGC 2 cut(s) 756, 775
Cfr13I GGNCC 2 cut(s) 26, 707
CseI GACGC 2 cut(s) 230, 368
Csp6I GTAC 3 cut(s) 192, 202, 539
CviAII CATG 2 cut(s) 376, 505
CviQI GTAC 3 cut(s) 192, 202, 539
DdeI CTNAG 2 cut(s) 15, 612
DpnI GATC 2 cut(s) 249, 503
DpnII GATC 2 cut(s) 247, 501
Eam1104I CTCTTC 2 cut(s) 183, 476
EarI CTCTTC 2 cut(s) 183, 476
EciI GGCGGA 2 cut(s) 175, 179
Eco57I CTGAAG 2 cut(s) 678, 684
EcoNI CCTNNNNNAGG 1 cut(s) 387
EcoO109I RGGNCCY 1 cut(s) 707
EcoRII CCWGG 2 cut(s) 385, 403
EcoT22I ATGCAT 1 cut(s) 120
FaeI CATG 2 cut(s) 379, 508
FaiI YATR 8 cut(s) 274, 276, 377, 395, 506, 530, 537, 737
FalI AAGNNNNNCTT 2 cut(s) 657, 689
FaqI GGGAC 1 cut(s) 578
FatI CATG 2 cut(s) 375, 504
FauNDI CATATG 1 cut(s) 274
FblI GTMKAC 2 cut(s) 394, 474
Fnu4HI GCNGC 6 cut(s) 162, 239, 647, 669, 776, 779
FokI GGATG 1 cut(s) 184
Fsp4HI GCNGC 6 cut(s) 162, 239, 647, 669, 776, 779
FspBI CTAG 2 cut(s) 228, 770
GlaI GCGC 2 cut(s) 755, 774
GluI GCNGC 6 cut(s) 162, 239, 647, 669, 776, 779
GsaI CCCAGC 1 cut(s) 143
GsuI CTGGAG 2 cut(s) 408, 678
HaeIII GGCC 2 cut(s) 28, 708
HapII CCGG 5 cut(s) 75, 224, 282, 449, 712
HgaI GACGC 2 cut(s) 230, 368
HhaI GCGC 2 cut(s) 756, 775
Hin1II CATG 2 cut(s) 379, 508
Hin6I GCGC 2 cut(s) 754, 773
HinP1I GCGC 2 cut(s) 754, 773
HindIII AAGCTT 1 cut(s) 38
HinfI GANTC 4 cut(s) 46, 148, 155, 797
HpaII CCGG 5 cut(s) 75, 224, 282, 449, 712
HphI GGTGA 3 cut(s) 122, 419, 502
Hpy166II GTNNAC 3 cut(s) 130, 395, 475
Hpy188I TCNGA 2 cut(s) 147, 255
Hpy188III TCNNGA 7 cut(s) 152, 245, 295, 449, 499, 505, 695
Hpy8I GTNNAC 3 cut(s) 130, 395, 475
Hpy99I CGWCG 2 cut(s) 94, 97
HpyAV CCTTC 3 cut(s) 228, 719, 782
HpyCH4III ACNGT 3 cut(s) 196, 427, 549
HpyCH4IV ACGT 1 cut(s) 204
HpyCH4V TGCA 5 cut(s) 118, 302, 359, 422, 629
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 67, 589, 668
HpyF3I CTNAG 2 cut(s) 15, 612
HpySE526I ACGT 1 cut(s) 204
Hsp92II CATG 2 cut(s) 379, 508
HspAI GCGC 2 cut(s) 754, 773
Kpn2I TCCGGA 1 cut(s) 448
Kzo9I GATC 2 cut(s) 247, 501
LguI GCTCTTC 2 cut(s) 183, 476
LmnI GCTCC 3 cut(s) 166, 406, 556
Lsp1109I GCAGC 4 cut(s) 225, 655, 787, 790
LweI GCATC 1 cut(s) 127
MaeI CTAG 2 cut(s) 228, 770
MaeII ACGT 1 cut(s) 204
MaeIII GTNAC 2 cut(s) 205, 371
MalI GATC 2 cut(s) 249, 503
MbiI CCGCTC 1 cut(s) 169
MboI GATC 2 cut(s) 247, 501
MboII GAAGA 5 cut(s) 78, 102, 170, 493, 710
MfeI CAATTG 2 cut(s) 303, 650
MhlI GDGCHC 1 cut(s) 618
MluCI AATT 2 cut(s) 303, 650
MlyI GAGTC 2 cut(s) 55, 164
MnlI CCTC 9 cut(s) 4, 109, 261, 383, 393, 456, 547, 555, 702
Mph1103I ATGCAT 1 cut(s) 120
MroI TCCGGA 1 cut(s) 448
MseI TTAA 2 cut(s) 512, 642
MslI CAYNNNNRTG 1 cut(s) 123
MspA1I CMGCKG 2 cut(s) 139, 470
MspCI CTTAAG 1 cut(s) 641
MspI CCGG 5 cut(s) 75, 224, 282, 449, 712
MspR9I CCNGG 5 cut(s) 76, 282, 387, 405, 712
MunI CAATTG 2 cut(s) 303, 650
MvaI CCWGG 2 cut(s) 387, 405
MwoI GCNNNNNNNGC 4 cut(s) 11, 67, 589, 668
NciI CCSGG 3 cut(s) 76, 282, 712
NdeI CATATG 1 cut(s) 274
NdeII GATC 2 cut(s) 247, 501
NlaIII CATG 2 cut(s) 379, 508
NlaIV GGNNCC 2 cut(s) 444, 709
NmuCI GTSAC 1 cut(s) 205
NsiI ATGCAT 1 cut(s) 120
NspI RCATGY 1 cut(s) 379
PagI TCATGA 1 cut(s) 504
PciSI GCTCTTC 2 cut(s) 183, 476
PcsI WCGNNNNNNNCGW 1 cut(s) 98
PfeI GAWTC 2 cut(s) 148, 797
Pfl23II CGTACG 1 cut(s) 201
PflFI GACNNNGTC 2 cut(s) 106, 211
PflMI CCANNNNNTGG 1 cut(s) 139
PfoI TCCNGGA 2 cut(s) 74, 385
PkrI GCNGC 6 cut(s) 163, 240, 648, 670, 777, 780
PleI GAGTC 2 cut(s) 54, 163
PpsI GAGTC 2 cut(s) 54, 163
Ppu21I YACGTR 1 cut(s) 205
Psp6I CCWGG 2 cut(s) 385, 403
PspFI CCCAGC 1 cut(s) 139
PspGI CCWGG 2 cut(s) 385, 403
PspLI CGTACG 1 cut(s) 201
PspN4I GGNNCC 2 cut(s) 444, 709
PspPI GGNCC 2 cut(s) 26, 707
PsyI GACNNNGTC 2 cut(s) 106, 211
PvuII CAGCTG 2 cut(s) 139, 470
RsaI GTAC 3 cut(s) 193, 203, 540
RsaNI GTAC 3 cut(s) 192, 202, 539
RseI CAYNNNNRTG 1 cut(s) 123
SapI GCTCTTC 2 cut(s) 183, 476
SaqAI TTAA 2 cut(s) 512, 642
SatI GCNGC 6 cut(s) 162, 239, 647, 669, 776, 779
Sau3AI GATC 2 cut(s) 247, 501
Sau96I GGNCC 2 cut(s) 26, 707
SchI GAGTC 2 cut(s) 55, 164
ScrFI CCNGG 5 cut(s) 76, 282, 387, 405, 712
SduI GDGCHC 1 cut(s) 618
SfaNI GCATC 1 cut(s) 127
SfcI CTRYAG 1 cut(s) 471
SmiMI CAYNNNNRTG 1 cut(s) 123
SmlI CTYRAG 1 cut(s) 641
SmoI CTYRAG 1 cut(s) 641
Sse9I AATT 2 cut(s) 303, 650
SsiI CCGC 5 cut(s) 161, 164, 169, 186, 646
SspMI CTAG 2 cut(s) 228, 770
StyD4I CCNGG 5 cut(s) 74, 280, 385, 403, 710
TaaI ACNGT 3 cut(s) 196, 427, 549
TaiI ACGT 1 cut(s) 207
TaqI TCGA 1 cut(s) 101
TasI AATT 2 cut(s) 303, 650
TauI GCSGC 2 cut(s) 164, 649
TfiI GAWTC 2 cut(s) 148, 797
Tru1I TTAA 2 cut(s) 512, 642
Tru9I TTAA 2 cut(s) 512, 642
TscAI CASTG 1 cut(s) 807
TseFI GTSAC 1 cut(s) 205
TseI GCWGC 4 cut(s) 238, 668, 775, 778
Tsp45I GTSAC 1 cut(s) 205
TspDTI ATGAA 3 cut(s) 170, 291, 756
TspRI CASTG 1 cut(s) 807
Tth111I GACNNNGTC 2 cut(s) 106, 211
Van91I CCANNNNNTGG 1 cut(s) 139
Vha464I CTTAAG 1 cut(s) 641
XagI CCTNNNNNAGG 1 cut(s) 387
XceI RCATGY 1 cut(s) 379
XmiI GTMKAC 2 cut(s) 394, 474
XspI CTAG 2 cut(s) 228, 770
Zsp2I ATGCAT 1 cut(s) 120
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.