Rw5G022680

Vesicle-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0003521
Physical Location & Seq
Reverse (-)
31574116 .. 31575828
1713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G022680.1

Sequence Viewer

Length: 822 bp
ATGGACCACCACCAAGACTACAGCCACTCCCACCACCACCACCACCACAGCCAGAGCAGCAGCCACCACCACCATCACCACCGGCACAAGTCCCACTCCGACGGCAACCTCTTCTCTCTCTGTCCGTTCTGGCAGTCAGGGTCCCAAACGTCGTCGTCTTCCTCTTCGACTCAGATCCTACAGAGCGGCGTCGACGGATCGAAGTCGAAGCCCAAAACGGTGTCGTCCGTCGCGAGATCGCTGCTCCCGCCCCGGCGCCGGCTCCGGCTCGACCCTGCCAGCAAACTGTACTTCCCCTATGAACCTGGGAAACAAGTGAAGAGCGCGATTCGGTTGCAGAACAGTAGCAAGTCTCATGTAGCTTTCAAGTTTCAAACTACTGCACCGAAAAGCTGTTACATGCGTCCTCCGGGTGGTATTCTTGCTCCAGGAGAGAGCATAATTGCTACTGTGTTTAGGTTTGTGGAACAACCCGACAGCAATGAGAAAAATGTGGATCAGAAGGCCAAGGTTAAGTTCAAAATTATGAGTCTGAAGGTGAAAGCTGAAACGGACTATGCTCCTGAGCTGTTTGATGAGCAAAAGGATCAAGTAGCAGTTGAGCGAATTTTGCGGGTTGTATTTTTAGATGCAGAGCACCCTACTCCTGCTCTAGAAAAACTGAAGAGGCAGTTGGCTGAAGCTGAGGCTGCACTTGAAGTGCGTAAGAAACCTCCCGCAGATTCAGGTCCTCGTGATGTTGGGGAAGGCCTTGTAATAGATGAATGGAAGGAGCGAAGGGAGAAATACCTGGCTCGGCAACAGGTTGAAGCAGTAGGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006970 GO:0008150 GO:0009628 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Motile_Sperm

Protein Analysis

273

Amino Acids

30.81

Weight (kDa)

9.44

Isoelectric Point (pI)

53.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Motile_Sperm PF00635 88 - 198 2e-24 MSP (Major sperm protein) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 255
AccBSI CCGCTC 1 cut(s) 186
AccI GTMKAC 1 cut(s) 192
AccII CGCG 2 cut(s) 233, 326
AciI CCGC 4 cut(s) 186, 248, 613, 717
AclWI GGATC 4 cut(s) 169, 205, 504, 594
AcsI RAATTY 1 cut(s) 606
AcuI CTGAAG 3 cut(s) 554, 683, 699
AcyI GRCGYC 2 cut(s) 189, 256
AfaI GTAC 1 cut(s) 290
AfiI CCNNNNNNNGG 2 cut(s) 258, 413
AgsI TTSAA 5 cut(s) 367, 374, 520, 698, 809
AjnI CCWGG 3 cut(s) 304, 427, 789
AjuI GAANNNNNNNTTGG 4 cut(s) 500, 532, 656, 688
AluBI AGCT 5 cut(s) 362, 393, 545, 568, 683
AluI AGCT 5 cut(s) 362, 393, 545, 568, 683
Alw21I GWGCWC 1 cut(s) 639
Alw26I GTCTC 1 cut(s) 357
AlwI GGATC 4 cut(s) 169, 205, 504, 594
AoxI GGCC 2 cut(s) 504, 748
ApeKI GCWGC 4 cut(s) 57, 60, 241, 689
ApoI RAATTY 1 cut(s) 606
AspLEI GCGC 2 cut(s) 258, 326
AspS9I GGNCC 3 cut(s) 4, 141, 728
AsuC2I CCSGG 2 cut(s) 253, 411
AsuHPI GGTGA 2 cut(s) 68, 550
AvaII GGWCC 3 cut(s) 4, 141, 728
BanI GGYRCC 1 cut(s) 255
BauI CACGAG 1 cut(s) 732
BbsI GAAGAC 1 cut(s) 150
Bbv12I GWGCWC 1 cut(s) 639
BbvCI CCTCAGC 1 cut(s) 684
BbvI GCAGC 4 cut(s) 69, 72, 228, 676
BccI CCATC 1 cut(s) 81
BceAI ACGGC 1 cut(s) 118
BcgI CGANNNNNNTGC 4 cut(s) 223, 257, 316, 350
BciT130I CCWGG 3 cut(s) 306, 429, 791
BcnI CCSGG 2 cut(s) 253, 411
BcoDI GTCTC 1 cut(s) 357
BfaI CTAG 1 cut(s) 653
BfmI CTRYAG 2 cut(s) 19, 179
BfoI RGCGCY 1 cut(s) 259
BisI GCNGC 5 cut(s) 58, 61, 187, 242, 690
BlsI GCNGC 5 cut(s) 59, 62, 188, 243, 691
Bme1390I CCNGG 5 cut(s) 253, 306, 411, 429, 791
Bme18I GGWCC 3 cut(s) 4, 141, 728
BmgT120I GGNCC 3 cut(s) 4, 141, 728
BmiI GGNNCC 4 cut(s) 142, 143, 257, 263
BmrFI CCNGG 5 cut(s) 253, 306, 411, 429, 791
BmsI GCATC 1 cut(s) 619
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 411
Bpu10I CCTNAGC 2 cut(s) 564, 684
BpuMI CCSGG 2 cut(s) 253, 411
BsaHI GRCGYC 2 cut(s) 189, 256
BsaJI CCNNGG 3 cut(s) 251, 305, 507
BsaXI ACNNNNNCTCC 2 cut(s) 11, 41
Bsc4I CCNNNNNNNGG 2 cut(s) 258, 413
Bse118I RCCGGY 2 cut(s) 81, 258
Bse3DI GCAATG 1 cut(s) 487
BseBI CCWGG 3 cut(s) 306, 429, 791
BseDI CCNNGG 3 cut(s) 251, 305, 507
BseLI CCNNNNNNNGG 2 cut(s) 258, 413
BseMI GCAATG 1 cut(s) 487
BseMII CTCAG 3 cut(s) 185, 555, 675
BseXI GCAGC 4 cut(s) 69, 72, 228, 676
BsgI GTGCAG 2 cut(s) 366, 675
Bsh1236I CGCG 2 cut(s) 233, 326
BshFI GGCC 2 cut(s) 506, 750
BshNI GGYRCC 1 cut(s) 255
BsiHKAI GWGCWC 1 cut(s) 639
BsiSI CCGG 5 cut(s) 82, 253, 259, 265, 410
BslFI GGGAC 2 cut(s) 76, 127
BslI CCNNNNNNNGG 2 cut(s) 258, 413
BsmAI GTCTC 1 cut(s) 357
BsmFI GGGAC 2 cut(s) 76, 127
BsnI GGCC 2 cut(s) 506, 750
Bsp1286I GDGCHC 1 cut(s) 639
Bsp143I GATC 5 cut(s) 174, 197, 236, 496, 586
Bsp68I TCGCGA 1 cut(s) 233
BspACI CCGC 4 cut(s) 186, 248, 613, 717
BspANI GGCC 2 cut(s) 506, 750
BspCNI CTCAG 3 cut(s) 184, 556, 676
BspFNI CGCG 2 cut(s) 233, 326
BspLI GGNNCC 4 cut(s) 142, 143, 257, 263
BspPI GGATC 4 cut(s) 169, 205, 504, 594
BspQI GCTCTTC 1 cut(s) 314
BspT107I GGYRCC 1 cut(s) 255
BsrBI CCGCTC 1 cut(s) 186
BsrDI GCAATG 1 cut(s) 487
BsrFI RCCGGY 2 cut(s) 81, 258
BssAI RCCGGY 2 cut(s) 81, 258
BssECI CCNNGG 3 cut(s) 251, 305, 507
BssMI GATC 5 cut(s) 174, 197, 236, 496, 586
BssNI GRCGYC 2 cut(s) 189, 256
BssSI CACGAG 1 cut(s) 732
BssT1I CCWWGG 1 cut(s) 507
Bst2BI CACGAG 1 cut(s) 732
Bst2UI CCWGG 3 cut(s) 306, 429, 791
Bst4CI ACNGT 4 cut(s) 220, 288, 344, 451
Bst6I CTCTTC 4 cut(s) 116, 169, 314, 659
BstACI GRCGYC 2 cut(s) 189, 256
BstC8I GCNNGC 2 cut(s) 260, 280
BstDEI CTNAG 3 cut(s) 171, 564, 684
BstFNI CGCG 2 cut(s) 233, 326
BstH2I RGCGCY 1 cut(s) 259
BstHHI GCGC 2 cut(s) 258, 326
BstKTI GATC 5 cut(s) 177, 200, 239, 499, 589
BstMAI GTCTC 1 cut(s) 357
BstMBI GATC 5 cut(s) 174, 197, 236, 496, 586
BstMWI GCNNNNNNNGC 4 cut(s) 57, 247, 610, 689
BstNI CCWGG 3 cut(s) 306, 429, 791
BstNSI RCATGY 1 cut(s) 403
BstSCI CCNGG 5 cut(s) 251, 304, 409, 427, 789
BstSFI CTRYAG 2 cut(s) 19, 179
BstUI CGCG 2 cut(s) 233, 326
BstV1I GCAGC 4 cut(s) 69, 72, 228, 676
BstV2I GAAGAC 1 cut(s) 150
BstX2I RGATCY 1 cut(s) 174
BstYI RGATCY 1 cut(s) 174
BsuRI GGCC 2 cut(s) 506, 750
BtuMI TCGCGA 1 cut(s) 233
Cac8I GCNNGC 2 cut(s) 260, 280
CfoI GCGC 2 cut(s) 258, 326
Cfr10I RCCGGY 2 cut(s) 81, 258
Cfr13I GGNCC 3 cut(s) 4, 141, 728
CseI GACGC 2 cut(s) 178, 392
Csp6I GTAC 1 cut(s) 289
CviAII CATG 2 cut(s) 356, 400
CviQI GTAC 1 cut(s) 289
DdeI CTNAG 3 cut(s) 171, 564, 684
DinI GGCGCC 1 cut(s) 257
DpnI GATC 5 cut(s) 176, 199, 238, 498, 588
DpnII GATC 5 cut(s) 174, 197, 236, 496, 586
Eam1104I CTCTTC 4 cut(s) 116, 169, 314, 659
EarI CTCTTC 4 cut(s) 116, 169, 314, 659
Eco130I CCWWGG 1 cut(s) 507
Eco147I AGGCCT 1 cut(s) 750
Eco47I GGWCC 3 cut(s) 4, 141, 728
Eco57I CTGAAG 3 cut(s) 554, 683, 699
EcoO109I RGGNCCY 2 cut(s) 141, 728
EcoRII CCWGG 3 cut(s) 304, 427, 789
EcoT14I CCWWGG 1 cut(s) 507
EgeI GGCGCC 1 cut(s) 257
EheI GGCGCC 1 cut(s) 257
ErhI CCWWGG 1 cut(s) 507
FaeI CATG 2 cut(s) 359, 403
FaiI YATR 6 cut(s) 300, 357, 401, 440, 527, 558
FaqI GGGAC 2 cut(s) 76, 127
FatI CATG 2 cut(s) 355, 399
FauI CCCGC 3 cut(s) 255, 606, 724
FblI GTMKAC 1 cut(s) 192
Fnu4HI GCNGC 5 cut(s) 58, 61, 187, 242, 690
Fsp4HI GCNGC 5 cut(s) 58, 61, 187, 242, 690
FspBI CTAG 1 cut(s) 653
GlaI GCGC 2 cut(s) 257, 325
GluI GCNGC 5 cut(s) 58, 61, 187, 242, 690
GsuI CTGGAG 1 cut(s) 411
HaeII RGCGCY 1 cut(s) 259
HaeIII GGCC 2 cut(s) 506, 750
HapII CCGG 5 cut(s) 82, 253, 259, 265, 410
HgaI GACGC 2 cut(s) 178, 392
HhaI GCGC 2 cut(s) 258, 326
Hin1I GRCGYC 2 cut(s) 189, 256
Hin1II CATG 2 cut(s) 359, 403
Hin6I GCGC 2 cut(s) 256, 324
HinP1I GCGC 2 cut(s) 256, 324
HincII GTYRAC 1 cut(s) 193
HindII GTYRAC 1 cut(s) 193
HinfI GANTC 4 cut(s) 169, 328, 529, 722
HpaII CCGG 5 cut(s) 82, 253, 259, 265, 410
HphI GGTGA 2 cut(s) 68, 550
Hpy166II GTNNAC 1 cut(s) 193
Hpy188I TCNGA 4 cut(s) 100, 174, 501, 534
Hpy188III TCNNGA 4 cut(s) 232, 563, 653, 734
Hpy8I GTNNAC 1 cut(s) 193
Hpy99I CGWCG 6 cut(s) 104, 154, 157, 194, 197, 233
HpyAV CCTTC 5 cut(s) 496, 529, 740, 763, 771
HpyCH4III ACNGT 4 cut(s) 220, 288, 344, 451
HpyCH4IV ACGT 1 cut(s) 149
HpyCH4V TGCA 4 cut(s) 337, 383, 632, 692
HpyF10VI GCNNNNNNNGC 4 cut(s) 57, 247, 610, 689
HpyF3I CTNAG 3 cut(s) 171, 564, 684
HpySE526I ACGT 1 cut(s) 149
Hsp92I GRCGYC 2 cut(s) 189, 256
Hsp92II CATG 2 cut(s) 359, 403
HspAI GCGC 2 cut(s) 256, 324
KasI GGCGCC 1 cut(s) 255
KflI GGGWCCC 1 cut(s) 141
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Kzo9I GATC 5 cut(s) 174, 197, 236, 496, 586
LguI GCTCTTC 1 cut(s) 314
LmnI GCTCC 5 cut(s) 249, 267, 430, 565, 772
Lsp1109I GCAGC 4 cut(s) 69, 72, 228, 676
LweI GCATC 1 cut(s) 619
MaeI CTAG 1 cut(s) 653
MaeII ACGT 1 cut(s) 149
MaeIII GTNAC 1 cut(s) 395
MalI GATC 5 cut(s) 176, 199, 238, 498, 588
MbiI CCGCTC 1 cut(s) 186
MboI GATC 5 cut(s) 174, 197, 236, 496, 586
MboII GAAGA 5 cut(s) 103, 150, 156, 331, 676
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 1 cut(s) 639
MluCI AATT 3 cut(s) 441, 522, 606
Mly113I GGCGCC 1 cut(s) 256
MlyI GAGTC 2 cut(s) 163, 538
MmeI TCCRAC 1 cut(s) 123
MnlI CCTC 7 cut(s) 119, 172, 417, 660, 679, 723, 741
MroNI GCCGGC 1 cut(s) 258
MseI TTAA 2 cut(s) 513, 820
MspI CCGG 5 cut(s) 82, 253, 259, 265, 410
MspR9I CCNGG 5 cut(s) 253, 306, 411, 429, 791
MvaI CCWGG 3 cut(s) 306, 429, 791
MvnI CGCG 2 cut(s) 233, 326
MwoI GCNNNNNNNGC 4 cut(s) 57, 247, 610, 689
NaeI GCCGGC 1 cut(s) 260
NarI GGCGCC 1 cut(s) 256
NciI CCSGG 2 cut(s) 253, 411
NdeII GATC 5 cut(s) 174, 197, 236, 496, 586
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 2 cut(s) 359, 403
NlaIV GGNNCC 4 cut(s) 142, 143, 257, 263
NmeAIII GCCGAG 1 cut(s) 775
NruI TCGCGA 1 cut(s) 233
NspI RCATGY 1 cut(s) 403
PceI AGGCCT 1 cut(s) 750
PciSI GCTCTTC 1 cut(s) 314
PcsI WCGNNNNNNNCGW 1 cut(s) 230
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 328, 722
PfoI TCCNGGA 1 cut(s) 427
PkrI GCNGC 5 cut(s) 59, 62, 188, 243, 691
PleI GAGTC 2 cut(s) 163, 537
PluTI GGCGCC 1 cut(s) 259
PpsI GAGTC 2 cut(s) 163, 537
PpuMI RGGWCCY 2 cut(s) 141, 728
Psp5II RGGWCCY 2 cut(s) 141, 728
Psp6I CCWGG 3 cut(s) 304, 427, 789
PspGI CCWGG 3 cut(s) 304, 427, 789
PspN4I GGNNCC 4 cut(s) 142, 143, 257, 263
PspPI GGNCC 3 cut(s) 4, 141, 728
PspPPI RGGWCCY 2 cut(s) 141, 728
PsuI RGATCY 1 cut(s) 174
RruI TCGCGA 1 cut(s) 233
RsaI GTAC 1 cut(s) 290
RsaNI GTAC 1 cut(s) 289
SalI GTCGAC 1 cut(s) 191
SapI GCTCTTC 1 cut(s) 314
SaqAI TTAA 2 cut(s) 513, 820
SatI GCNGC 5 cut(s) 58, 61, 187, 242, 690
Sau3AI GATC 5 cut(s) 174, 197, 236, 496, 586
Sau96I GGNCC 3 cut(s) 4, 141, 728
SchI GAGTC 2 cut(s) 163, 538
ScrFI CCNGG 5 cut(s) 253, 306, 411, 429, 791
SduI GDGCHC 1 cut(s) 639
SfaNI GCATC 1 cut(s) 619
SfcI CTRYAG 2 cut(s) 19, 179
SfoI GGCGCC 1 cut(s) 257
SgrDI CGTCGACG 1 cut(s) 191
SinI GGWCC 3 cut(s) 4, 141, 728
Sse9I AATT 3 cut(s) 441, 522, 606
SseBI AGGCCT 1 cut(s) 750
SsiI CCGC 4 cut(s) 186, 248, 613, 717
SspDI GGCGCC 1 cut(s) 255
SspMI CTAG 1 cut(s) 653
StuI AGGCCT 1 cut(s) 750
StyD4I CCNGG 5 cut(s) 251, 304, 409, 427, 789
StyI CCWWGG 1 cut(s) 507
TaaI ACNGT 4 cut(s) 220, 288, 344, 451
TaiI ACGT 1 cut(s) 152
TaqI TCGA 5 cut(s) 167, 192, 200, 206, 270
TasI AATT 3 cut(s) 441, 522, 606
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 189
TfiI GAWTC 2 cut(s) 328, 722
Tru1I TTAA 2 cut(s) 513, 820
Tru9I TTAA 2 cut(s) 513, 820
TseI GCWGC 4 cut(s) 57, 60, 241, 689
TspDTI ATGAA 2 cut(s) 315, 777
TspGWI ACGGA 4 cut(s) 114, 210, 217, 566
VpaK11BI GGWCC 3 cut(s) 4, 141, 728
XapI RAATTY 1 cut(s) 606
XbaI TCTAGA 1 cut(s) 652
XceI RCATGY 1 cut(s) 403
XmiI GTMKAC 1 cut(s) 192
XspI CTAG 1 cut(s) 653
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.